Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25712.t1 | AES71738.2 | 85.9 | 92 | 13 | 0 | 1 | 92 | 11 | 102 | 1.40E-37 | 165.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25712.t1 | F4JDJ0 | 38.7 | 75 | 45 | 1 | 1 | 75 | 17 | 90 | 3.8e-07 | 55.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25712.t1 | G7J841 | 85.9 | 92 | 13 | 0 | 1 | 92 | 11 | 102 | 1.0e-37 | 165.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052902 | MS.gene25712 | 0.834506 | 2.88E-56 | -1.69E-46 |
| MS.gene053356 | MS.gene25712 | 0.837541 | 4.89E-57 | -1.69E-46 |
| MS.gene058426 | MS.gene25712 | 0.808519 | 2.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25712.t1 | MTR_3g082210 | 85.870 | 92 | 13 | 0 | 1 | 92 | 11 | 102 | 2.03e-46 | 144 |
| MS.gene25712.t1 | MTR_3g082250 | 69.072 | 97 | 21 | 3 | 1 | 92 | 22 | 114 | 2.45e-36 | 119 |
| MS.gene25712.t1 | MTR_1g041690 | 59.794 | 97 | 32 | 3 | 1 | 92 | 11 | 105 | 6.75e-29 | 100 |
| MS.gene25712.t1 | MTR_5g007070 | 90.566 | 53 | 5 | 0 | 8 | 60 | 44 | 96 | 6.34e-28 | 97.4 |
| MS.gene25712.t1 | MTR_3g082260 | 57.732 | 97 | 34 | 3 | 1 | 92 | 11 | 105 | 3.74e-27 | 95.9 |
| MS.gene25712.t1 | MTR_0635s0010 | 49.091 | 55 | 28 | 0 | 1 | 55 | 19 | 73 | 5.50e-12 | 56.2 |
| MS.gene25712.t1 | MTR_2g027600 | 49.091 | 55 | 28 | 0 | 1 | 55 | 21 | 75 | 6.23e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAAGAAGAACCAATTA+TGG | + | 24213:24579-24598 | MS.gene25712:CDS | 25.0% |
| ! | AAGGATCAAATAGAAGTAAT+CGG | + | 24213:24460-24479 | MS.gene25712:CDS | 25.0% |
| ! | AATCATGTCCAAAAATATTC+AGG | + | 24213:24406-24425 | MS.gene25712:intron | 25.0% |
| ! | TAGTTTGGATAATTAAGCTA+TGG | - | 24213:24387-24406 | MS.gene25712:intergenic | 25.0% |
| !!! | ATTTTTGGACATGATTAGTT+TGG | - | 24213:24402-24421 | MS.gene25712:intergenic | 25.0% |
| ! | TTTCCACTCCTGAATATTTT+TGG | - | 24213:24417-24436 | MS.gene25712:intergenic | 30.0% |
| AAGAAGAACCAATTATGGCA+TGG | + | 24213:24584-24603 | MS.gene25712:CDS | 35.0% | |
| GAAATTCTGTCATGCAGAAT+TGG | + | 24213:24525-24544 | MS.gene25712:CDS | 35.0% | |
| TGGTGAAGTGAAAAAGAAAG+AGG | + | 24213:24555-24574 | MS.gene25712:CDS | 35.0% | |
| TGTCCAAAAATATTCAGGAG+TGG | + | 24213:24411-24430 | MS.gene25712:intron | 35.0% | |
| !! | TAGCAGTTTTGTGAGTCTAA+CGG | - | 24213:24500-24519 | MS.gene25712:intergenic | 35.0% |
| AAGATTGCAGTTGGAGTTTC+AGG | + | 24213:24302-24321 | MS.gene25712:CDS | 40.0% | |
| AGCAATACAAGGAGATAGCA+AGG | + | 24213:24441-24460 | MS.gene25712:CDS | 40.0% | |
| TGAGGATAACCAGTTACACA+AGG | - | 24213:24610-24629 | MS.gene25712:intergenic | 40.0% | |
| ACACAAGGCCATGCCATAAT+TGG | - | 24213:24595-24614 | MS.gene25712:intergenic | 45.0% | |
| CATGCAGAATTGGTGAGTGT+TGG | + | 24213:24535-24554 | MS.gene25712:CDS | 45.0% | |
| TGTCCCTGTGTGTGAGATAA+GGG | + | 24213:24636-24655 | MS.gene25712:CDS | 45.0% | |
| TTATCTCACACACAGGGACA+GGG | - | 24213:24636-24655 | MS.gene25712:intergenic | 45.0% | |
| ! | GTGGAAAGTGCAGCAATACA+AGG | + | 24213:24430-24449 | MS.gene25712:CDS | 45.0% |
| !! | TTTGAATTAAAAATAATTAA+TGG | - | 24213:24352-24371 | MS.gene25712:intergenic | 5.0% |
| CGTCCCTTATCTCACACACA+GGG | - | 24213:24642-24661 | MS.gene25712:intergenic | 50.0% | |
| CTGTCCCTGTGTGTGAGATA+AGG | + | 24213:24635-24654 | MS.gene25712:CDS | 50.0% | |
| CTTATCTCACACACAGGGAC+AGG | - | 24213:24637-24656 | MS.gene25712:intergenic | 50.0% | |
| TCGTCCCTTATCTCACACAC+AGG | - | 24213:24643-24662 | MS.gene25712:intergenic | 50.0% | |
| !! | ATGGCATGGCCTTGTGTAAC+TGG | + | 24213:24598-24617 | MS.gene25712:CDS | 50.0% |
| CACACAGGGACAGGGTAATG+AGG | - | 24213:24628-24647 | MS.gene25712:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24213 | gene | 24299 | 24680 | 24299 | ID=MS.gene25712 |
| 24213 | mRNA | 24299 | 24680 | 24299 | ID=MS.gene25712.t1;Parent=MS.gene25712 |
| 24213 | exon | 24299 | 24323 | 24299 | ID=MS.gene25712.t1.exon1;Parent=MS.gene25712.t1 |
| 24213 | CDS | 24299 | 24323 | 24299 | ID=cds.MS.gene25712.t1;Parent=MS.gene25712.t1 |
| 24213 | exon | 24428 | 24680 | 24428 | ID=MS.gene25712.t1.exon2;Parent=MS.gene25712.t1 |
| 24213 | CDS | 24428 | 24680 | 24428 | ID=cds.MS.gene25712.t1;Parent=MS.gene25712.t1 |
| Gene Sequence |
| Protein sequence |
