AlfalfaGEDB | MS.gene25714

M. sativa cultivar XinJiangDaYe / MS.gene25714

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25714.t1	AES71738.2	85.4	103	15	0	1	103	1	103	4.70E-42	180.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25714.t1	F4JDJ0	40.0	85	50	1	1	85	7	90	9.0e-10	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25714.t1	G7J841	85.4	103	15	0	1	103	1	103	3.4e-42	180.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052902	MS.gene25714	0.834252	3.34E-56	-1.69E-46
MS.gene053356	MS.gene25714	0.83652	8.92E-57	-1.69E-46
MS.gene058426	MS.gene25714	0.807348	5.28E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25714.t1	MTR_3g082210	85.437	103	15	0	1	103	1	103	2.88e-53	162
MS.gene25714.t1	MTR_3g082250	69.444	108	24	3	1	103	12	115	1.02e-42	135
MS.gene25714.t1	MTR_1g041690	60.185	108	36	3	1	103	1	106	5.70e-35	115
MS.gene25714.t1	MTR_3g082260	58.333	108	38	3	1	103	1	106	3.61e-33	111
MS.gene25714.t1	MTR_5g007070	70.732	82	12	1	1	70	15	96	1.30e-31	107
MS.gene25714.t1	MTR_5g084660	43.200	125	47	5	1	102	10	133	6.57e-18	73.6
MS.gene25714.t1	MTR_0635s0010	50.769	65	32	0	1	65	9	73	1.05e-16	68.6
MS.gene25714.t1	MTR_2g027600	50.769	65	32	0	1	65	11	75	1.10e-16	68.9
MS.gene25714.t1	MTR_6g082190	47.170	53	28	0	1	53	1	53	5.10e-12	59.7
MS.gene25714.t1	MTR_6g082330	43.750	64	36	0	1	64	11	74	4.61e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25714.t1	AT3G20180	40.000	85	50	1	1	85	7	90	3.53e-14	63.5
MS.gene25714.t1	AT3G20180	40.000	85	50	1	1	85	9	92	3.90e-14	63.5
MS.gene25714.t1	AT3G20180	40.000	85	50	1	1	85	11	94	4.49e-14	63.5
MS.gene25714.t1	AT3G20180	44.737	76	40	1	1	74	30	105	5.32e-14	63.5
MS.gene25714.t1	AT5G48290	48.649	74	35	1	1	71	1	74	7.15e-14	63.9
MS.gene25714.t1	AT5G48290	48.649	74	35	1	1	71	11	84	7.90e-14	63.9
MS.gene25714.t1	AT3G07600	44.928	69	38	0	1	69	11	79	1.80e-13	62.8

Find 0 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGAAAGAAGAACCAATTA+TGG	+	24220:11904-11923	MS.gene25714:CDS	25.0%
!	AAGGATCAAATAGAAGTAAT+CGG	+	24220:11785-11804	MS.gene25714:CDS	25.0%
!	AATCATGTCCAAAAATATTC+AGG	+	24220:11731-11750	MS.gene25714:intron	25.0%
!	TAGTTTGGATAATTAAGCTA+TGG	-	24220:11712-11731	None:intergenic	25.0%
!!!	ATTTTTGGACATGATTAGTT+TGG	-	24220:11727-11746	None:intergenic	25.0%
!	TTTCCACTCCTGAATATTTT+TGG	-	24220:11742-11761	None:intergenic	30.0%
	AAGAAGAACCAATTATGGCA+TGG	+	24220:11909-11928	MS.gene25714:CDS	35.0%
	GAAATTCTGTCATGCAGAAT+TGG	+	24220:11850-11869	MS.gene25714:CDS	35.0%
	TGGTGAAGTGAAAAAGAAAG+AGG	+	24220:11880-11899	MS.gene25714:CDS	35.0%
	TGTCCAAAAATATTCAGGAG+TGG	+	24220:11736-11755	MS.gene25714:intron	35.0%
!!	TAGCAGTTTTGTGAGTCTAA+CGG	-	24220:11825-11844	None:intergenic	35.0%
	AAAGCCATGAAGATTGCAGT+TGG	+	24220:11618-11637	MS.gene25714:CDS	40.0%
	AACTCCAACTGCAATCTTCA+TGG	-	24220:11625-11644	None:intergenic	40.0%
	AACTGCAATCTTCATGGCTT+TGG	-	24220:11619-11638	None:intergenic	40.0%
	AAGATTGCAGTTGGAGTTTC+AGG	+	24220:11627-11646	MS.gene25714:CDS	40.0%
	AGCAATACAAGGAGATAGCA+AGG	+	24220:11766-11785	MS.gene25714:CDS	40.0%
	TGAGGATAACCAGTTACACA+AGG	-	24220:11935-11954	None:intergenic	40.0%
!!!	TGGCTTTGGACCTTGATTTT+TGG	-	24220:11605-11624	None:intergenic	40.0%
	ACACAAGGCCATGCCATAAT+TGG	-	24220:11920-11939	None:intergenic	45.0%
	CATGCAGAATTGGTGAGTGT+TGG	+	24220:11860-11879	MS.gene25714:CDS	45.0%
	TGTCCCTGTGTGTGAGATAA+GGG	+	24220:11961-11980	MS.gene25714:CDS	45.0%
	TTATCTCACACACAGGGACA+GGG	-	24220:11961-11980	None:intergenic	45.0%
!	GTGGAAAGTGCAGCAATACA+AGG	+	24220:11755-11774	MS.gene25714:CDS	45.0%
!!	TTTGAATTAAAAATAATTAA+TGG	-	24220:11677-11696	None:intergenic	5.0%
	CGTCCCTTATCTCACACACA+GGG	-	24220:11967-11986	None:intergenic	50.0%
	CTGTCCCTGTGTGTGAGATA+AGG	+	24220:11960-11979	MS.gene25714:CDS	50.0%
	CTTATCTCACACACAGGGAC+AGG	-	24220:11962-11981	None:intergenic	50.0%
	TCGTCCCTTATCTCACACAC+AGG	-	24220:11968-11987	None:intergenic	50.0%
!!	ATGGCATGGCCTTGTGTAAC+TGG	+	24220:11923-11942	MS.gene25714:CDS	50.0%
	CACACAGGGACAGGGTAATG+AGG	-	24220:11953-11972	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
24220	gene	11594	12009	11594	ID=MS.gene25714
24220	mRNA	11594	12009	11594	ID=MS.gene25714.t1;Parent=MS.gene25714
24220	exon	11594	11648	11594	ID=MS.gene25714.t1.exon1;Parent=MS.gene25714.t1
24220	CDS	11594	11648	11594	ID=cds.MS.gene25714.t1;Parent=MS.gene25714.t1
24220	exon	11753	12009	11753	ID=MS.gene25714.t1.exon2;Parent=MS.gene25714.t1
24220	CDS	11753	12009	11753	ID=cds.MS.gene25714.t1;Parent=MS.gene25714.t1

Gene Sequence

>MS.gene25714

ATGCACAGCCAAAAATCAAGGTCCAAAGCCATGAAGATTGCAGTTGGAGTTTCAGGTAATAAAATTCACTGATTTTGTCACCATTAATTATTTTTAATTCAAAATTTGTTCATAACCATAGCTTAATTATCCAAACTAATCATGTCCAAAAATATTCAGGAGTGGAAAGTGCAGCAATACAAGGAGATAGCAAGGATCAAATAGAAGTAATCGGAGAACAAATAGACTCCGTTAGACTCACAAAACTGCTAAGAAAGAAATTCTGTCATGCAGAATTGGTGAGTGTTGGTGAAGTGAAAAAGAAAGAGGAAAAGAAAGAAGAACCAATTATGGCATGGCCTTGTGTAACTGGTTATCCTCATTACCCTGTCCCTGTGTGTGAGATAAGGGACGACTCTTCTTGTTCCATCATGTGA

Protein sequence

>MS.gene25714.t1

MHSQKSRSKAMKIAVGVSGVESAAIQGDSKDQIEVIGEQIDSVRLTKLLRKKFCHAELVSVGEVKKKEEKKEEPIMAWPCVTGYPHYPVPVCEIRDDSSCSIM