Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25836.t1 | GAU44153.1 | 64.3 | 185 | 49 | 5 | 14 | 181 | 176 | 360 | 6.50E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25836.t1 | A0A2Z6NPY8 | 64.3 | 185 | 49 | 5 | 14 | 181 | 176 | 360 | 4.7e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene25836 | 0.803834 | 2.89E-49 | -1.69E-46 |
| MS.gene050139 | MS.gene25836 | 0.803747 | 3.01E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene25836 | 0.801062 | 1.07E-48 | -1.69E-46 |
| MS.gene059124 | MS.gene25836 | 0.800784 | 1.23E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25836.t1 | MTR_3g079783 | 78.378 | 111 | 17 | 1 | 78 | 181 | 184 | 294 | 2.09e-53 | 172 |
| MS.gene25836.t1 | MTR_3g079820 | 67.460 | 126 | 32 | 4 | 64 | 181 | 2 | 126 | 2.09e-46 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25836.t1 | AT5G20935 | 64.557 | 79 | 25 | 2 | 103 | 179 | 38 | 115 | 9.36e-26 | 95.9 |
Find 35 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAAATCAACTGGAATATT+TGG | 0.198763 | 3.2:+70123820 | None:intergenic |
| ACGTTTGTACAAGCCAAAAT+TGG | 0.210526 | 3.2:+70123678 | None:intergenic |
| CTGTTGTGGAACATTGATTT+TGG | 0.228912 | 3.2:+70123846 | None:intergenic |
| TGGAGCTTCAATGATAATAA+TGG | 0.263065 | 3.2:+70123738 | None:intergenic |
| TAATTACTGAGCCAGATCTA+AGG | 0.312435 | 3.2:+70123341 | None:intergenic |
| AAACGTTCAAAGTTGGATAA+AGG | 0.314200 | 3.2:-70123766 | MS.gene25836:CDS |
| TCGGTTTATTGCCCCAATTT+TGG | 0.319384 | 3.2:-70123691 | MS.gene25836:CDS |
| ATTGGAACTTATATTATTGA+TGG | 0.349289 | 3.2:-70123378 | MS.gene25836:CDS |
| GGTCAAAACTGCAGCTAATT+CGG | 0.362866 | 3.2:-70123710 | MS.gene25836:CDS |
| CGTTTGTACAAGCCAAAATT+GGG | 0.376043 | 3.2:+70123679 | None:intergenic |
| TTGGTGCCGACCTTCAAATA+TGG | 0.377926 | 3.2:+70123865 | None:intergenic |
| GGAGCTTCAATGATAATAAT+GGG | 0.378832 | 3.2:+70123739 | None:intergenic |
| AACGTTCAAAGTTGGATAAA+GGG | 0.431347 | 3.2:-70123765 | MS.gene25836:CDS |
| ATATTTCAAGCCCTTAGATC+TGG | 0.456940 | 3.2:-70123352 | MS.gene25836:CDS |
| CGTGACCTCAGCAAATCAAC+TGG | 0.460489 | 3.2:+70123811 | None:intergenic |
| TACCACCAAGCCATATTTGA+AGG | 0.463739 | 3.2:-70123875 | MS.gene25836:CDS |
| TGGGCAGTGCGGCGCAGCAT+TGG | 0.466372 | 3.2:-70123396 | MS.gene25836:CDS |
| TATCATTGAAGCTCCAAAGA+TGG | 0.502814 | 3.2:-70123731 | MS.gene25836:CDS |
| CTTGGTGGTAGAGCAAGAAA+TGG | 0.514075 | 3.2:+70123888 | None:intergenic |
| GGAATATTTGGTATCTGTTG+TGG | 0.533879 | 3.2:+70123832 | None:intergenic |
| TCAATCCTATCATATGAATG+AGG | 0.535136 | 3.2:+70124066 | None:intergenic |
| AATTACTGAGCCAGATCTAA+GGG | 0.536515 | 3.2:+70123342 | None:intergenic |
| CAATCCTATCATATGAATGA+GGG | 0.550534 | 3.2:+70124067 | None:intergenic |
| GACCTTCAAATATGGCTTGG+TGG | 0.555798 | 3.2:+70123873 | None:intergenic |
| GATAAAGTTATAAACAGTGT+AGG | 0.575222 | 3.2:-70123931 | MS.gene25836:CDS |
| GCCGACCTTCAAATATGGCT+TGG | 0.578715 | 3.2:+70123870 | None:intergenic |
| ATATTCCAGTTGATTTGCTG+AGG | 0.581675 | 3.2:-70123816 | MS.gene25836:CDS |
| TGAAGAGAAATCGTCACTGA+AGG | 0.602801 | 3.2:-70123965 | MS.gene25836:CDS |
| ACCAAGCCATATTTGAAGGT+CGG | 0.616443 | 3.2:-70123871 | MS.gene25836:CDS |
| TTGTACAAACGTGGTGATGT+TGG | 0.626789 | 3.2:-70123667 | MS.gene25836:CDS |
| ATCCTATCATATGAATGAGG+GGG | 0.635069 | 3.2:+70124069 | None:intergenic |
| AATCCTATCATATGAATGAG+GGG | 0.646986 | 3.2:+70124068 | None:intergenic |
| ACAAACGTGGTGATGTTGGA+AGG | 0.684402 | 3.2:-70123663 | MS.gene25836:intron |
| AAGAAGATGTGTGGGCAGTG+CGG | 0.684625 | 3.2:-70123407 | MS.gene25836:CDS |
| GTTTGTACAAGCCAAAATTG+GGG | 0.698986 | 3.2:+70123680 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTCTAAAGATTAATTAAT+TGG | + | chr3.2:70123894-70123913 | None:intergenic | 15.0% |
| !! | ATTGGAACTTATATTATTGA+TGG | - | chr3.2:70124050-70124069 | MS.gene25836:CDS | 20.0% |
| !! | GCATAATCAAATGAATAAAA+TGG | - | chr3.2:70123945-70123964 | MS.gene25836:CDS | 20.0% |
| ! | GATAAAGTTATAAACAGTGT+AGG | - | chr3.2:70123497-70123516 | MS.gene25836:intron | 25.0% |
| ! | TGATGAAAAATTAAGAGATG+AGG | + | chr3.2:70123798-70123817 | None:intergenic | 25.0% |
| AAAAAAGCAAGAAGATGTGT+GGG | - | chr3.2:70124013-70124032 | MS.gene25836:CDS | 30.0% | |
| AAACGTTCAAAGTTGGATAA+AGG | - | chr3.2:70123662-70123681 | MS.gene25836:intron | 30.0% | |
| AACGTTCAAAGTTGGATAAA+GGG | - | chr3.2:70123663-70123682 | MS.gene25836:intron | 30.0% | |
| AATCCTATCATATGAATGAG+GGG | + | chr3.2:70123363-70123382 | None:intergenic | 30.0% | |
| AGCAAATCAACTGGAATATT+TGG | + | chr3.2:70123611-70123630 | None:intergenic | 30.0% | |
| CAATCCTATCATATGAATGA+GGG | + | chr3.2:70123364-70123383 | None:intergenic | 30.0% | |
| GGAGCTTCAATGATAATAAT+GGG | + | chr3.2:70123692-70123711 | None:intergenic | 30.0% | |
| TCAATCCTATCATATGAATG+AGG | + | chr3.2:70123365-70123384 | None:intergenic | 30.0% | |
| TGGAGCTTCAATGATAATAA+TGG | + | chr3.2:70123693-70123712 | None:intergenic | 30.0% | |
| ACGTTTGTACAAGCCAAAAT+TGG | + | chr3.2:70123753-70123772 | None:intergenic | 35.0% | |
| ATATTTCAAGCCCTTAGATC+TGG | - | chr3.2:70124076-70124095 | MS.gene25836:CDS | 35.0% | |
| ATCCTATCATATGAATGAGG+GGG | + | chr3.2:70123362-70123381 | None:intergenic | 35.0% | |
| CAAAAAAGCAAGAAGATGTG+TGG | - | chr3.2:70124012-70124031 | MS.gene25836:CDS | 35.0% | |
| CGTTTGTACAAGCCAAAATT+GGG | + | chr3.2:70123752-70123771 | None:intergenic | 35.0% | |
| GCAAGAAAAACGTTCAAAGT+TGG | - | chr3.2:70123655-70123674 | MS.gene25836:intron | 35.0% | |
| GGAATATTTGGTATCTGTTG+TGG | + | chr3.2:70123599-70123618 | None:intergenic | 35.0% | |
| GTTTGTACAAGCCAAAATTG+GGG | + | chr3.2:70123751-70123770 | None:intergenic | 35.0% | |
| TATCATTGAAGCTCCAAAGA+TGG | - | chr3.2:70123697-70123716 | MS.gene25836:CDS | 35.0% | |
| ! | CAAATTTTACACACACTAGC+AGG | - | chr3.2:70123832-70123851 | MS.gene25836:CDS | 35.0% |
| ! | CGTTTTTCTTGCAACTCTTT+TGG | + | chr3.2:70123647-70123666 | None:intergenic | 35.0% |
| !! | ATATTCCAGTTGATTTGCTG+AGG | - | chr3.2:70123612-70123631 | MS.gene25836:intron | 35.0% |
| !!! | AATTTTGGCTTGTACAAACG+TGG | - | chr3.2:70123752-70123771 | MS.gene25836:CDS | 35.0% |
| !!! | CTGTTGTGGAACATTGATTT+TGG | + | chr3.2:70123585-70123604 | None:intergenic | 35.0% |
| ACCAAGCCATATTTGAAGGT+CGG | - | chr3.2:70123557-70123576 | MS.gene25836:intron | 40.0% | |
| ATATGAATGAGGGGGAAAGA+AGG | + | chr3.2:70123354-70123373 | None:intergenic | 40.0% | |
| GCAGGAGCAACTAATTGATT+TGG | - | chr3.2:70123850-70123869 | MS.gene25836:CDS | 40.0% | |
| GGTCAAAACTGCAGCTAATT+CGG | - | chr3.2:70123718-70123737 | MS.gene25836:CDS | 40.0% | |
| TACCACCAAGCCATATTTGA+AGG | - | chr3.2:70123553-70123572 | MS.gene25836:intron | 40.0% | |
| TGAAGAGAAATCGTCACTGA+AGG | - | chr3.2:70123463-70123482 | MS.gene25836:intron | 40.0% | |
| TTCCCCCTCATTCATATGAT+AGG | - | chr3.2:70123357-70123376 | MS.gene25836:CDS | 40.0% | |
| ! | TCGGTTTATTGCCCCAATTT+TGG | - | chr3.2:70123737-70123756 | MS.gene25836:CDS | 40.0% |
| ! | TTGTACAAACGTGGTGATGT+TGG | - | chr3.2:70123761-70123780 | MS.gene25836:CDS | 40.0% |
| ! | ACAAACGTGGTGATGTTGGA+AGG | - | chr3.2:70123765-70123784 | MS.gene25836:CDS | 45.0% |
| ! | CTTGGTGGTAGAGCAAGAAA+TGG | + | chr3.2:70123543-70123562 | None:intergenic | 45.0% |
| ! | GACCTTCAAATATGGCTTGG+TGG | + | chr3.2:70123558-70123577 | None:intergenic | 45.0% |
| !! | GCTGCAGTTTTGACCATCTT+TGG | + | chr3.2:70123713-70123732 | None:intergenic | 45.0% |
| !! | TTGGTGCCGACCTTCAAATA+TGG | + | chr3.2:70123566-70123585 | None:intergenic | 45.0% |
| AAGAAGATGTGTGGGCAGTG+CGG | - | chr3.2:70124021-70124040 | MS.gene25836:CDS | 50.0% | |
| CGTGACCTCAGCAAATCAAC+TGG | + | chr3.2:70123620-70123639 | None:intergenic | 50.0% | |
| GCCGACCTTCAAATATGGCT+TGG | + | chr3.2:70123561-70123580 | None:intergenic | 50.0% | |
| TGGGCAGTGCGGCGCAGCAT+TGG | - | chr3.2:70124032-70124051 | MS.gene25836:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 70123344 | 70124106 | 70123344 | ID=MS.gene25836 |
| chr3.2 | mRNA | 70123344 | 70124106 | 70123344 | ID=MS.gene25836.t1;Parent=MS.gene25836 |
| chr3.2 | exon | 70123664 | 70124106 | 70123664 | ID=MS.gene25836.t1.exon1;Parent=MS.gene25836.t1 |
| chr3.2 | CDS | 70123664 | 70124106 | 70123664 | ID=cds.MS.gene25836.t1;Parent=MS.gene25836.t1 |
| chr3.2 | exon | 70123344 | 70123446 | 70123344 | ID=MS.gene25836.t1.exon2;Parent=MS.gene25836.t1 |
| chr3.2 | CDS | 70123344 | 70123446 | 70123344 | ID=cds.MS.gene25836.t1;Parent=MS.gene25836.t1 |
| Gene Sequence |
| Protein sequence |
