Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26875.t1 | XP_013469824.1 | 96.8 | 124 | 2 | 1 | 1 | 122 | 1 | 124 | 2.30E-48 | 201.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26875.t1 | B7FMI8 | 96.8 | 124 | 2 | 1 | 1 | 122 | 1 | 124 | 1.7e-48 | 201.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050201 | MS.gene26875 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene26875 | 0.803266 | 3.78E-49 | -1.69E-46 |
| MS.gene061176 | MS.gene26875 | 0.805571 | 1.25E-49 | -1.69E-46 |
| MS.gene061177 | MS.gene26875 | 0.805211 | 1.49E-49 | -1.69E-46 |
| MS.gene061178 | MS.gene26875 | 0.805418 | 1.35E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26875.t1 | MTR_1g103550 | 97.581 | 124 | 1 | 1 | 1 | 122 | 1 | 124 | 2.63e-85 | 244 |
| MS.gene26875.t1 | MTR_5g006430 | 74.561 | 114 | 24 | 2 | 1 | 114 | 1 | 109 | 1.48e-55 | 169 |
| MS.gene26875.t1 | MTR_8g021190 | 47.170 | 106 | 49 | 3 | 2 | 102 | 30 | 133 | 1.39e-29 | 104 |
| MS.gene26875.t1 | MTR_8g013990 | 44.037 | 109 | 50 | 4 | 2 | 102 | 62 | 167 | 2.98e-25 | 94.7 |
| MS.gene26875.t1 | MTR_2g101140 | 47.253 | 91 | 42 | 2 | 2 | 88 | 62 | 150 | 2.46e-24 | 92.4 |
| MS.gene26875.t1 | MTR_3g105340 | 40.541 | 111 | 59 | 4 | 1 | 107 | 10 | 117 | 4.12e-22 | 84.7 |
| MS.gene26875.t1 | MTR_4g125010 | 46.575 | 73 | 38 | 1 | 1 | 73 | 23 | 94 | 4.92e-22 | 85.5 |
| MS.gene26875.t1 | MTR_4g125030 | 45.333 | 75 | 40 | 1 | 1 | 75 | 21 | 94 | 4.56e-20 | 79.3 |
| MS.gene26875.t1 | MTR_3g109860 | 38.462 | 91 | 51 | 2 | 2 | 88 | 36 | 125 | 4.69e-18 | 74.7 |
| MS.gene26875.t1 | MTR_1g014930 | 43.836 | 73 | 39 | 1 | 3 | 73 | 17 | 89 | 1.94e-17 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26875.t1 | AT3G22540 | 70.270 | 111 | 28 | 3 | 1 | 110 | 1 | 107 | 2.20e-48 | 150 |
| MS.gene26875.t1 | AT4G14819 | 63.636 | 110 | 34 | 2 | 1 | 110 | 1 | 104 | 6.16e-42 | 134 |
| MS.gene26875.t1 | AT5G25840 | 42.105 | 114 | 58 | 2 | 2 | 107 | 42 | 155 | 1.45e-25 | 95.1 |
| MS.gene26875.t1 | AT1G72510 | 48.913 | 92 | 41 | 2 | 2 | 88 | 33 | 123 | 1.81e-25 | 94.4 |
| MS.gene26875.t1 | AT1G72510 | 48.913 | 92 | 41 | 2 | 2 | 88 | 33 | 123 | 1.81e-25 | 94.4 |
| MS.gene26875.t1 | AT1G79770 | 44.037 | 109 | 55 | 2 | 1 | 105 | 50 | 156 | 1.50e-24 | 92.0 |
| MS.gene26875.t1 | AT1G54095 | 36.220 | 127 | 71 | 3 | 2 | 118 | 15 | 141 | 3.94e-22 | 85.5 |
| MS.gene26875.t1 | AT5G20670 | 39.048 | 105 | 53 | 3 | 3 | 97 | 23 | 126 | 3.05e-20 | 80.9 |
| MS.gene26875.t1 | AT2G09970 | 39.130 | 92 | 51 | 1 | 2 | 88 | 33 | 124 | 4.38e-19 | 78.2 |
| MS.gene26875.t1 | AT2G25780 | 32.075 | 106 | 68 | 1 | 6 | 107 | 39 | 144 | 1.06e-15 | 68.9 |
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCCAAATTTGATGGAAAA+TGG | 0.263831 | 1.4:+80094219 | MS.gene26875:CDS |
| GATGTGAAATCCAAATTTGA+TGG | 0.416112 | 1.4:+80094212 | MS.gene26875:CDS |
| CTTGAATACTTCTTTGATGA+AGG | 0.426664 | 1.4:-80094437 | None:intergenic |
| CTTCATCAAAGAAGTATTCA+AGG | 0.467585 | 1.4:+80094438 | MS.gene26875:CDS |
| TTTGATGGAAAATGGTTATG+TGG | 0.481592 | 1.4:+80094227 | MS.gene26875:CDS |
| CCTTCTGCTACTCTAACTGC+AGG | 0.505097 | 1.4:-80094362 | None:intergenic |
| TTGATGGAAAATGGTTATGT+GGG | 0.531455 | 1.4:+80094228 | MS.gene26875:CDS |
| TCACCAACCACTTCTCAAGT+TGG | 0.545624 | 1.4:+80094461 | MS.gene26875:CDS |
| ATCAAATTTGGATTTCACAT+CGG | 0.557162 | 1.4:-80094210 | None:intergenic |
| AGAGATGAAGTAATGAGTGG+AGG | 0.566320 | 1.4:+80094269 | MS.gene26875:CDS |
| GTGAGAGATGAAGTAATGAG+TGG | 0.578986 | 1.4:+80094266 | MS.gene26875:CDS |
| AGAATCTCCAACTTGAGAAG+TGG | 0.629154 | 1.4:-80094468 | None:intergenic |
| CCTGCAGTTAGAGTAGCAGA+AGG | 0.629928 | 1.4:+80094362 | MS.gene26875:CDS |
| TCTCCAACTTGAGAAGTGGT+TGG | 0.638457 | 1.4:-80094464 | None:intergenic |
| TCAGCAAAGTGTGAATGTTG+TGG | 0.681770 | 1.4:+80094155 | MS.gene26875:CDS |
| CTTCTGCTACTCTAACTGCA+GGG | 0.694897 | 1.4:-80094361 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATCCAAATTTGATGGAAAA+TGG | + | chr1.4:80094219-80094238 | MS.gene26875:CDS | 25.0% |
| ! | ATCAAATTTGGATTTCACAT+CGG | - | chr1.4:80094213-80094232 | None:intergenic | 25.0% |
| !! | TAACCATTTTCCATCAAATT+TGG | - | chr1.4:80094225-80094244 | None:intergenic | 25.0% |
| CTTGAATACTTCTTTGATGA+AGG | - | chr1.4:80094440-80094459 | None:intergenic | 30.0% | |
| GATGTGAAATCCAAATTTGA+TGG | + | chr1.4:80094212-80094231 | MS.gene26875:CDS | 30.0% | |
| TTGATGGAAAATGGTTATGT+GGG | + | chr1.4:80094228-80094247 | MS.gene26875:CDS | 30.0% | |
| TTTGATGGAAAATGGTTATG+TGG | + | chr1.4:80094227-80094246 | MS.gene26875:CDS | 30.0% | |
| !! | CTTCATCAAAGAAGTATTCA+AGG | + | chr1.4:80094438-80094457 | MS.gene26875:CDS | 30.0% |
| AGGTAAAAAACAATGGGACA+TGG | + | chr1.4:80094289-80094308 | MS.gene26875:CDS | 35.0% | |
| GAGTGGAGGTAAAAAACAAT+GGG | + | chr1.4:80094283-80094302 | MS.gene26875:CDS | 35.0% | |
| TGAGTGGAGGTAAAAAACAA+TGG | + | chr1.4:80094282-80094301 | MS.gene26875:CDS | 35.0% | |
| AGAATCTCCAACTTGAGAAG+TGG | - | chr1.4:80094471-80094490 | None:intergenic | 40.0% | |
| AGAGATGAAGTAATGAGTGG+AGG | + | chr1.4:80094269-80094288 | MS.gene26875:CDS | 40.0% | |
| GTGAGAGATGAAGTAATGAG+TGG | + | chr1.4:80094266-80094285 | MS.gene26875:CDS | 40.0% | |
| TCAGCAAAGTGTGAATGTTG+TGG | + | chr1.4:80094155-80094174 | MS.gene26875:CDS | 40.0% | |
| CTTCTGCTACTCTAACTGCA+GGG | - | chr1.4:80094364-80094383 | None:intergenic | 45.0% | |
| TCACCAACCACTTCTCAAGT+TGG | + | chr1.4:80094461-80094480 | MS.gene26875:CDS | 45.0% | |
| TCTCCAACTTGAGAAGTGGT+TGG | - | chr1.4:80094467-80094486 | None:intergenic | 45.0% | |
| CCTGCAGTTAGAGTAGCAGA+AGG | + | chr1.4:80094362-80094381 | MS.gene26875:CDS | 50.0% | |
| CCTTCTGCTACTCTAACTGC+AGG | - | chr1.4:80094365-80094384 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 80094143 | 80094511 | 80094143 | ID=MS.gene26875 |
| chr1.4 | mRNA | 80094143 | 80094511 | 80094143 | ID=MS.gene26875.t1;Parent=MS.gene26875 |
| chr1.4 | exon | 80094143 | 80094511 | 80094143 | ID=MS.gene26875.t1.exon1;Parent=MS.gene26875.t1 |
| chr1.4 | CDS | 80094143 | 80094511 | 80094143 | ID=cds.MS.gene26875.t1;Parent=MS.gene26875.t1 |
| Gene Sequence |
| Protein sequence |
