AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061178


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061178.t1 MTR_4g121590 95.455 176 8 0 1 176 201 376 7.03e-126 358
MS.gene061178.t1 MTR_4g121570 77.714 175 39 0 1 175 201 375 9.81e-103 300
MS.gene061178.t1 MTR_4g073090 78.409 176 38 0 1 176 201 376 3.12e-102 299
MS.gene061178.t1 MTR_3g013890 40.805 174 87 3 7 172 225 390 4.31e-42 145
MS.gene061178.t1 MTR_3g102730 40.805 174 87 3 7 172 225 390 4.70e-42 145
MS.gene061178.t1 MTR_3g102760 40.116 172 101 2 2 172 220 390 9.38e-41 141
MS.gene061178.t1 MTR_1g018610 40.351 171 101 1 2 172 254 423 1.10e-40 142
MS.gene061178.t1 MTR_1g018580 39.766 171 102 1 2 172 195 364 5.54e-40 139
MS.gene061178.t1 MTR_3g102770 39.759 166 99 1 7 172 225 389 1.09e-39 139
MS.gene061178.t1 MTR_1g018590 39.181 171 103 1 2 172 225 394 1.59e-39 138
MS.gene061178.t1 MTR_3g463660 35.429 175 107 3 3 172 202 375 6.65e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061178.t1 AT4G24220 35.673 171 109 1 2 172 219 388 3.05e-36 129
MS.gene061178.t1 AT4G24220 35.673 171 109 1 2 172 218 387 3.82e-36 129
MS.gene061178.t1 AT5G58750 32.571 175 114 3 3 173 204 378 1.53e-23 95.9
MS.gene061178.t1 AT5G58750 32.571 175 114 3 3 173 212 386 1.64e-23 95.9

Find 0 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTAATAGTGTGAAATTCT+GGG - 32464:11270-11289 MS.gene061178:CDS 25.0%
CGTTTAATAGTGTGAAATTC+TGG - 32464:11269-11288 MS.gene061178:CDS 30.0%
TGAAAAGGAAGAGTTTGATT+TGG - 32464:11063-11082 MS.gene061178:CDS 30.0%
TGAAACTCAAATCGAAAAAC+TGG + 32464:11193-11212 MS.gene061178:intergenic 30.0%
! ACAAGCAATTTTCTCCAATT+TGG + 32464:11155-11174 MS.gene061178:intergenic 30.0%
CAAGCGTTTAATTGCACAAA+TGG - 32464:10968-10987 MS.gene061178:CDS 35.0%
CATTTGTGCAATTAAACGCT+TGG + 32464:10970-10989 MS.gene061178:intergenic 35.0%
CTATTTCCTGGATAACGAAA+CGG + 32464:10857-10876 MS.gene061178:intergenic 35.0%
TGGACTTCATAAGACCAAAT+TGG - 32464:11138-11157 MS.gene061178:CDS 35.0%
!! TTTTCACATGGAAGAGTATG+TGG - 32464:10996-11015 MS.gene061178:CDS 35.0%
!!! AATTGCTTGTTTTGAAGCCT+TGG - 32464:11165-11184 MS.gene061178:CDS 35.0%
!!! GACTTTTGCTGTTAAGTTTG+TGG - 32464:11033-11052 MS.gene061178:CDS 35.0%
AAATCGAAAAACTGGCACCA+AGG + 32464:11185-11204 MS.gene061177:intergenic 40.0%
CGTTTCGTTATCCAGGAAAT+AGG - 32464:10855-10874 MS.gene061178:CDS 40.0%
GTGCAGTTTATGCGTGATAA+GGG - 32464:11085-11104 MS.gene061178:CDS 40.0%
GTTTGTGGAGTTGGATGAAA+AGG - 32464:11048-11067 MS.gene061178:CDS 40.0%
TCCAAAGTGTACCTATTTCC+TGG + 32464:10869-10888 MS.gene061178:intergenic 40.0%
TCCAGGAAATAGGTACACTT+TGG - 32464:10865-10884 MS.gene061178:CDS 40.0%
TGCTGTTAAGTTTGTGGAGT+TGG - 32464:11039-11058 MS.gene061178:CDS 40.0%
! GCAATTAAACGCTTGGTTCT+TGG + 32464:10963-10982 MS.gene061178:intergenic 40.0%
! TGAGATTTGGGATCAGATTG+TGG - 32464:11108-11127 MS.gene061178:CDS 40.0%
!! CAAATGGTGACGTTTTCACA+TGG - 32464:10984-11003 MS.gene061178:CDS 40.0%
AACGAAACGGTAATCCTACC+TGG + 32464:10844-10863 MS.gene061177:intergenic 45.0%
AATTCTGGGTGATGAAGCTG+AGG - 32464:11284-11303 MS.gene061178:CDS 45.0%
ATTCTGGGTGATGAAGCTGA+GGG - 32464:11285-11304 MS.gene061178:CDS 45.0%
GATCAGATTGTGGAGGAGTA+TGG - 32464:11118-11137 MS.gene061178:CDS 45.0%
GCGTGATAAGGGTGAGATTT+GGG - 32464:11096-11115 MS.gene061178:CDS 45.0%
GGATTACCGTTTCGTTATCC+AGG - 32464:10848-10867 MS.gene061178:CDS 45.0%
GGTGCAGTTTATGCGTGATA+AGG - 32464:11084-11103 MS.gene061178:CDS 45.0%
TAATCCTACCTGGCGACATA+TGG + 32464:10834-10853 MS.gene061178:intergenic 45.0%
TGCGTGATAAGGGTGAGATT+TGG - 32464:11095-11114 MS.gene061178:CDS 45.0%
! CACATGGAAGAGTATGTGGA+AGG - 32464:11000-11019 MS.gene061178:CDS 45.0%
! GATTTGGGATCAGATTGTGG+AGG - 32464:11111-11130 MS.gene061178:CDS 45.0%
! GGAGCATTTCTGCGATATGA+CGG - 32464:10886-10905 MS.gene061178:CDS 45.0%
! GTGGGCTGCAGTTACAAAGA+AGG - 32464:10937-10956 MS.gene061178:CDS 50.0%
GCTCGCAAAGCAGCATGTGT+GGG - 32464:10919-10938 MS.gene061178:CDS 55.0%
TGCTCGCAAAGCAGCATGTG+TGG - 32464:10918-10937 MS.gene061178:CDS 55.0%
ATGCGCTGCCATATGTCGCC+AGG - 32464:10823-10842 MS.gene061178:CDS 60.0%
GCTGCCATATGTCGCCAGGT+AGG - 32464:10827-10846 MS.gene061178:CDS 60.0%


Chromosome Type Strat End Strand Name
32464 gene 10809 11339 10809 ID=MS.gene061178
32464 mRNA 10809 11339 10809 ID=MS.gene061178.t1;Parent=MS.gene061178
32464 exon 10809 11339 10809 ID=MS.gene061178.t1.exon1;Parent=MS.gene061178.t1
32464 CDS 10809 11339 10809 ID=cds.MS.gene061178.t1;Parent=MS.gene061178.t1
Gene Sequence

>MS.gene061178

ATGGTACTAGCGACATGCGCTGCCATATGTCGCCAGGTAGGATTACCGTTTCGTTATCCAGGAAATAGGTACACTTTGGAGCATTTCTGCGATATGACGGATGCTCGTGTGCTCGCAAAGCAGCATGTGTGGGCTGCAGTTACAAAGAAGGCCAAGAACCAAGCGTTTAATTGCACAAATGGTGACGTTTTCACATGGAAGAGTATGTGGAAGGTGCTTTGTAAGACTTTTGCTGTTAAGTTTGTGGAGTTGGATGAAAAGGAAGAGTTTGATTTGGTGCAGTTTATGCGTGATAAGGGTGAGATTTGGGATCAGATTGTGGAGGAGTATGGACTTCATAAGACCAAATTGGAGAAAATTGCTTGTTTTGAAGCCTTGGTGCCAGTTTTTCGATTTGAGTTTCAACTTGTATCTAGTATGAATAAGAGTAAAAATTATGAGTTTTTTGAGTATGCTGAGACGTTTAATAGTGTGAAATTCTGGGTGATGAAGCTGAGGGAGATGAATCTCATACCCATCTACGAACATTGA

Protein sequence

>MS.gene061178.t1

MVLATCAAICRQVGLPFRYPGNRYTLEHFCDMTDARVLAKQHVWAAVTKKAKNQAFNCTNGDVFTWKSMWKVLCKTFAVKFVELDEKEEFDLVQFMRDKGEIWDQIVEEYGLHKTKLEKIACFEALVPVFRFEFQLVSSMNKSKNYEFFEYAETFNSVKFWVMKLREMNLIPIYEH