AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061176


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061176.t1 MTR_4g121590 95.455 176 8 0 1 176 201 376 7.03e-126 358
MS.gene061176.t1 MTR_4g121570 77.714 175 39 0 1 175 201 375 9.81e-103 300
MS.gene061176.t1 MTR_4g073090 78.409 176 38 0 1 176 201 376 3.12e-102 299
MS.gene061176.t1 MTR_3g013890 40.805 174 87 3 7 172 225 390 4.31e-42 145
MS.gene061176.t1 MTR_3g102730 40.805 174 87 3 7 172 225 390 4.70e-42 145
MS.gene061176.t1 MTR_3g102760 40.116 172 101 2 2 172 220 390 9.38e-41 141
MS.gene061176.t1 MTR_1g018610 40.351 171 101 1 2 172 254 423 1.10e-40 142
MS.gene061176.t1 MTR_1g018580 39.766 171 102 1 2 172 195 364 5.54e-40 139
MS.gene061176.t1 MTR_3g102770 39.759 166 99 1 7 172 225 389 1.09e-39 139
MS.gene061176.t1 MTR_1g018590 39.181 171 103 1 2 172 225 394 1.59e-39 138
MS.gene061176.t1 MTR_3g463660 35.429 175 107 3 3 172 202 375 6.65e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061176.t1 AT4G24220 35.673 171 109 1 2 172 219 388 3.05e-36 129
MS.gene061176.t1 AT4G24220 35.673 171 109 1 2 172 218 387 3.82e-36 129
MS.gene061176.t1 AT5G58750 32.571 175 114 3 3 173 204 378 1.53e-23 95.9
MS.gene061176.t1 AT5G58750 32.571 175 114 3 3 173 212 386 1.64e-23 95.9

Find 0 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTAATAGTGTGAAATTCT+GGG - 32462:16544-16563 MS.gene061176:CDS 25.0%
CGTTTAATAGTGTGAAATTC+TGG - 32462:16543-16562 MS.gene061176:CDS 30.0%
TGAAAAGGAAGAGTTTGATT+TGG - 32462:16337-16356 MS.gene061176:CDS 30.0%
TGAAACTCAAATCGAAAAAC+TGG + 32462:16467-16486 None:intergenic 30.0%
! ACAAGCAATTTTCTCCAATT+TGG + 32462:16429-16448 None:intergenic 30.0%
CAAGCGTTTAATTGCACAAA+TGG - 32462:16242-16261 MS.gene061176:CDS 35.0%
CATTTGTGCAATTAAACGCT+TGG + 32462:16244-16263 None:intergenic 35.0%
CTATTTCCTGGATAACGAAA+CGG + 32462:16131-16150 None:intergenic 35.0%
TGGACTTCATAAGACCAAAT+TGG - 32462:16412-16431 MS.gene061176:CDS 35.0%
!! TTTTCACATGGAAGAGTATG+TGG - 32462:16270-16289 MS.gene061176:CDS 35.0%
!!! AATTGCTTGTTTTGAAGCCT+TGG - 32462:16439-16458 MS.gene061176:CDS 35.0%
!!! GACTTTTGCTGTTAAGTTTG+TGG - 32462:16307-16326 MS.gene061176:CDS 35.0%
AAATCGAAAAACTGGCACCA+AGG + 32462:16459-16478 None:intergenic 40.0%
CGTTTCGTTATCCAGGAAAT+AGG - 32462:16129-16148 MS.gene061176:CDS 40.0%
GTGCAGTTTATGCGTGATAA+GGG - 32462:16359-16378 MS.gene061176:CDS 40.0%
GTTTGTGGAGTTGGATGAAA+AGG - 32462:16322-16341 MS.gene061176:CDS 40.0%
TCCAAAGTGTACCTATTTCC+TGG + 32462:16143-16162 None:intergenic 40.0%
TCCAGGAAATAGGTACACTT+TGG - 32462:16139-16158 MS.gene061176:CDS 40.0%
TGCTGTTAAGTTTGTGGAGT+TGG - 32462:16313-16332 MS.gene061176:CDS 40.0%
! GCAATTAAACGCTTGGTTCT+TGG + 32462:16237-16256 None:intergenic 40.0%
! TGAGATTTGGGATCAGATTG+TGG - 32462:16382-16401 MS.gene061176:CDS 40.0%
!! CAAATGGTGACGTTTTCACA+TGG - 32462:16258-16277 MS.gene061176:CDS 40.0%
AACGAAACGGTAATCCTACC+TGG + 32462:16118-16137 None:intergenic 45.0%
AATTCTGGGTGATGAAGCTG+AGG - 32462:16558-16577 MS.gene061176:CDS 45.0%
ATTCTGGGTGATGAAGCTGA+GGG - 32462:16559-16578 MS.gene061176:CDS 45.0%
GATCAGATTGTGGAGGAGTA+TGG - 32462:16392-16411 MS.gene061176:CDS 45.0%
GCGTGATAAGGGTGAGATTT+GGG - 32462:16370-16389 MS.gene061176:CDS 45.0%
GGATTACCGTTTCGTTATCC+AGG - 32462:16122-16141 MS.gene061176:CDS 45.0%
GGTGCAGTTTATGCGTGATA+AGG - 32462:16358-16377 MS.gene061176:CDS 45.0%
TAATCCTACCTGGCGACATA+TGG + 32462:16108-16127 None:intergenic 45.0%
TGCGTGATAAGGGTGAGATT+TGG - 32462:16369-16388 MS.gene061176:CDS 45.0%
! CACATGGAAGAGTATGTGGA+AGG - 32462:16274-16293 MS.gene061176:CDS 45.0%
! GATTTGGGATCAGATTGTGG+AGG - 32462:16385-16404 MS.gene061176:CDS 45.0%
! GGAGCATTTCTGCGATATGA+CGG - 32462:16160-16179 MS.gene061176:CDS 45.0%
! GTGGGCTGCAGTTACAAAGA+AGG - 32462:16211-16230 MS.gene061176:CDS 50.0%
GCTCGCAAAGCAGCATGTGT+GGG - 32462:16193-16212 MS.gene061176:CDS 55.0%
TGCTCGCAAAGCAGCATGTG+TGG - 32462:16192-16211 MS.gene061176:CDS 55.0%
ATGCGCTGCCATATGTCGCC+AGG - 32462:16097-16116 MS.gene061176:CDS 60.0%
GCTGCCATATGTCGCCAGGT+AGG - 32462:16101-16120 MS.gene061176:CDS 60.0%


Chromosome Type Strat End Strand Name
32462 gene 16083 16613 16083 ID=MS.gene061176
32462 mRNA 16083 16613 16083 ID=MS.gene061176.t1;Parent=MS.gene061176
32462 exon 16083 16613 16083 ID=MS.gene061176.t1.exon1;Parent=MS.gene061176.t1
32462 CDS 16083 16613 16083 ID=cds.MS.gene061176.t1;Parent=MS.gene061176.t1
Gene Sequence

>MS.gene061176

ATGGTACTAGCGACATGCGCTGCCATATGTCGCCAGGTAGGATTACCGTTTCGTTATCCAGGAAATAGGTACACTTTGGAGCATTTCTGCGATATGACGGATGCTCGTGTGCTCGCAAAGCAGCATGTGTGGGCTGCAGTTACAAAGAAGGCCAAGAACCAAGCGTTTAATTGCACAAATGGTGACGTTTTCACATGGAAGAGTATGTGGAAGGTGCTTTGTAAGACTTTTGCTGTTAAGTTTGTGGAGTTGGATGAAAAGGAAGAGTTTGATTTGGTGCAGTTTATGCGTGATAAGGGTGAGATTTGGGATCAGATTGTGGAGGAGTATGGACTTCATAAGACCAAATTGGAGAAAATTGCTTGTTTTGAAGCCTTGGTGCCAGTTTTTCGATTTGAGTTTCAACTTGTATCTAGTATGAATAAGAGTAAAAATTATGAGTTTTTTGAGTATGCTGAGACGTTTAATAGTGTGAAATTCTGGGTGATGAAGCTGAGGGAGATGAATCTCATACCCATCTACGAACATTGA

Protein sequence

>MS.gene061176.t1

MVLATCAAICRQVGLPFRYPGNRYTLEHFCDMTDARVLAKQHVWAAVTKKAKNQAFNCTNGDVFTWKSMWKVLCKTFAVKFVELDEKEEFDLVQFMRDKGEIWDQIVEEYGLHKTKLEKIACFEALVPVFRFEFQLVSSMNKSKNYEFFEYAETFNSVKFWVMKLREMNLIPIYEH