AlfalfaGEDB | MS.gene27035

M. sativa cultivar XinJiangDaYe / MS.gene27035

KEGG: -
GO: -
PFAM: PF06217.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27035.t1	XP_003589387.1	98.5	340	4	1	1	339	1	340	1.30E-160	575.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27035.t1	Q8L999	66.2	358	86	15	1	339	1	342	6.7e-86	318.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27035.t1	G7I6G1	98.5	340	4	1	1	339	1	340	9.1e-161	575.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene27035.t1	TF	BBR-BPC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene000525	MS.gene27035	PPI
MS.gene27035	MS.gene79458	PPI
MS.gene27035	MS.gene030481	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27035.t1	MTR_1g023660	98.529	340	4	1	1	339	1	340	0.0	699
MS.gene27035.t1	MTR_8g099780	49.713	348	127	10	2	339	3	312	8.30e-104	307

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27035.t1	AT5G42520	65.084	358	90	15	1	339	1	342	1.12e-149	425
MS.gene27035.t1	AT5G42520	63.966	358	90	15	1	339	1	338	1.89e-145	414
MS.gene27035.t1	AT5G42520	62.543	291	84	11	65	339	1	282	2.13e-116	338
MS.gene27035.t1	AT2G21240	48.830	342	115	7	5	339	8	296	6.31e-100	297
MS.gene27035.t1	AT2G21240	48.830	342	115	7	5	339	8	296	6.31e-100	297
MS.gene27035.t1	AT4G38910	45.266	338	120	7	5	339	8	283	8.17e-93	278
MS.gene27035.t1	AT4G38910	45.253	316	111	6	24	339	5	258	1.31e-86	261
MS.gene27035.t1	AT2G35550	55.932	118	52	0	222	339	109	226	1.43e-45	155
MS.gene27035.t1	AT2G35550	55.932	118	52	0	222	339	109	226	1.43e-45	155
MS.gene27035.t1	AT2G35550	55.932	118	52	0	222	339	109	226	1.43e-45	155

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Find 89 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCATGCTCGAGTAGG+TGG	0.599819	1.1:-1186345	MS.gene27035:CDS
AAAGACCATTGGGCCAAACA+TGG	0.604624	1.1:-1186237	MS.gene27035:CDS
AAAGGCTGATTGGAAACCTC+AGG	0.538629	1.1:-1186532	MS.gene27035:CDS
AACAAAAGGCATGCTCGAGT+AGG	0.658830	1.1:-1186348	MS.gene27035:CDS
AACCACCTAGGCGTGCCAAA+CGG	0.540428	1.1:-1186731	MS.gene27035:CDS
AAGTGGATATACCGATAAAG+TGG	0.604637	1.1:+1186383	None:intergenic
AATATGGGTGATTCTTCTTA+CGG	0.455899	1.1:-1186816	MS.gene27035:CDS
AATCACCCATATTAGGCAAA+TGG	0.448313	1.1:+1186826	None:intergenic
AATGGTCTTTAAGGTCAACC+GGG	0.672212	1.1:+1186250	None:intergenic
AATGGTTTACCTGTTGCGGA+AGG	0.606017	1.1:+1186844	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATATGGGTGATTCTTCTTA+CGG	-	chr1.1:1186727-1186746	MS.gene27035:CDS	30.0%
AGATCAATATAAATCTGCTC+AGG	-	chr1.1:1186241-1186260	MS.gene27035:CDS	30.0%
AGTAGTGAGGAAATGATAAA+TGG	-	chr1.1:1186955-1186974	MS.gene27035:CDS	30.0%
ATCTAGAAAAGTCAAGAAAG+AGG	-	chr1.1:1186879-1186898	MS.gene27035:CDS	30.0%
CTTTCAATAAACTACTTAGC+CGG	-	chr1.1:1187241-1187260	MS.gene27035:intron	30.0%
GAAGCAAATTATGTCAATCA+TGG	-	chr1.1:1186432-1186451	MS.gene27035:CDS	30.0%
GATCAATATAAATCTGCTCA+GGG	-	chr1.1:1186242-1186261	MS.gene27035:CDS	30.0%
GTAAACCATTTGCCTAATAT+GGG	-	chr1.1:1186712-1186731	MS.gene27035:CDS	30.0%
TAAGAAGAATCACCCATATT+AGG	+	chr1.1:1186727-1186746	None:intergenic	30.0%
TCTAGAAAAGTCAAGAAAGA+GGG	-	chr1.1:1186880-1186899	MS.gene27035:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	1186209	1187356	1186209	ID=MS.gene27035
chr1.1	mRNA	1186209	1187356	1186209	ID=MS.gene27035.t1;Parent=MS.gene27035
chr1.1	exon	1187297	1187356	1187297	ID=MS.gene27035.t1.exon1;Parent=MS.gene27035.t1
chr1.1	CDS	1187297	1187356	1187297	ID=cds.MS.gene27035.t1;Parent=MS.gene27035.t1
chr1.1	exon	1186209	1187168	1186209	ID=MS.gene27035.t1.exon2;Parent=MS.gene27035.t1
chr1.1	CDS	1186209	1187168	1186209	ID=cds.MS.gene27035.t1;Parent=MS.gene27035.t1

Gene Sequence

>MS.gene27035

ATGGACGATCGTGAAAATGGAAGGCACAAAGCAGATCAATATAAATCTGCTCAGGGACAGGTATATGAAACACGCCTCTGCTCAGAAATAAATTTAAGTTTGCATGAACATAACAGTTGTTAAAAGTGCTGCATATACCTAAAAGGGTGCTTTCTCTACTAATATTCTTCTAAAAATTGCTTTTGCAGTGGTTAATGCAACAGCATCACCAGCATCCTTCCATGAAGCAAATTATGTCAATCATGGCTGAACGAGATGCAGCCATTCAAGAAAGAAATCTGGCCCTCTCTGAGAAAAAGGCAGCACTTGCAGAACGTGACATGGCATTCCTGCAGCGAGATACTGCAATTGCGGAACGGAATAATGCACTTATGGAAAGAGATAATGCGATTGCAACCCTTCAGTTTCGAGAAAATGCTTTGGCCAATGGTGGTATGTCATCATGCCCTCCAGGATGTCAAATCTCACGGGGAGTTAAGCATATACATCACCTTCCGCAACAGGTAAACCATTTGCCTAATATGGGTGATTCTTCTTACGGCACACGTGAATTGCACACAACTGATGCCCTCCCAGCAGCCCCCATTTCTTCAGAGGTTGGGAAACCACCTAGGCGTGCCAAACGGCCAAAGGAAAGTAAGTCTGATTCACCTAACAAGAAGACTCCAAAATCTAGAAAAGTCAAGAAAGAGGGTGATGATTTAAACAAGACAATGTTTGCCAACAACAAAGAGCTTGAATGGAAGAGTAGTGAGGAAATGATAAATGGAGATGATGATCTTAACAAGCAGTTAGCAGCCTCAAAGGCTGATTGGAAACCTCAGGACTTGGCATTGAACCAAGTTGCATACGATGATTCAACCATGCCAGCACCAGCATGCTCTTGTACTGGTGTTCTGAGACAGTGCTACAAATGGGGTAATGGAGGTTGGCAATCTGCTTGCTGTACAACCACTTTATCGGTATATCCACTTCCAGCTGTGCCCAACAAAAGGCATGCTCGAGTAGGTGGTAGAAAAATGAGTGGAAGTGCTTTCAATAAACTACTTAGCCGGCTTGCAGCTGAAGGGCATGATCTCTCACACCCGGTTGACCTTAAAGACCATTGGGCCAAACATGGTACAAATCGATACATCACAATAAAGTAG

Protein sequence

>MS.gene27035.t1

MDDRENGRHKADQYKSAQGQWLMQQHHQHPSMKQIMSIMAERDAAIQERNLALSEKKAALAERDMAFLQRDTAIAERNNALMERDNAIATLQFRENALANGGMSSCPPGCQISRGVKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPISSEVGKPPRRAKRPKESKSDSPNKKTPKSRKVKKEGDDLNKTMFANNKELEWKSSEEMINGDDDLNKQLAASKADWKPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKRHARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK