Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27184.t1 | XP_013462892.1 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 3.50E-90 | 340.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27184.t1 | Q94AU9 | 63.6 | 176 | 55 | 3 | 1 | 172 | 1 | 171 | 7.7e-54 | 211.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27184.t1 | A0A072V552 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 2.5e-90 | 340.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051171 | MS.gene27184 | 0.857363 | 1.72E-62 | -1.69E-46 |
| MS.gene051242 | MS.gene27184 | 0.856415 | 3.27E-62 | -1.69E-46 |
| MS.gene051244 | MS.gene27184 | 0.868267 | 7.41E-66 | -1.69E-46 |
| MS.gene053330 | MS.gene27184 | 0.84671 | 1.83E-59 | -1.69E-46 |
| MS.gene053971 | MS.gene27184 | 0.837434 | 5.21E-57 | -1.69E-46 |
| MS.gene055370 | MS.gene27184 | 0.848983 | 4.33E-60 | -1.69E-46 |
| MS.gene05625 | MS.gene27184 | 0.812362 | 4.41E-51 | -1.69E-46 |
| MS.gene056305 | MS.gene27184 | 0.838857 | 2.24E-57 | -1.69E-46 |
| MS.gene056633 | MS.gene27184 | 0.925186 | 2.58E-90 | -1.69E-46 |
| MS.gene056664 | MS.gene27184 | 0.853454 | 2.37E-61 | -1.69E-46 |
| MS.gene057196 | MS.gene27184 | 0.821456 | 4.00E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene27184 | 0.916691 | 1.31E-85 | -1.69E-46 |
| MS.gene059512 | MS.gene27184 | 0.848214 | 7.07E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27184.t1 | MTR_2g024300 | 96.532 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 1.39e-126 | 353 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27184.t1 | AT4G04330 | 70.270 | 148 | 41 | 2 | 25 | 172 | 27 | 171 | 2.79e-72 | 215 |
Find 34 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGCCAGCTGGCCAAAA+AGG | 0.282297 | 2.1:+64910467 | None:intergenic |
| TGTCCCATTCAAAATCCTTT+TGG | 0.366494 | 2.1:+64909700 | None:intergenic |
| TGAAGCATATGTTGAGCTAA+TGG | 0.380963 | 2.1:-64910071 | MS.gene27184:CDS |
| TTGTTGGTGCATGGTGTAGC+AGG | 0.403399 | 2.1:+64910497 | None:intergenic |
| ACTCTAACACGAGGCTTATG+AGG | 0.410584 | 2.1:-64909532 | MS.gene27184:CDS |
| ATTAGCTCAACATATGCTTC+AGG | 0.434378 | 2.1:+64910073 | None:intergenic |
| GAGCTCCTTCTCTTGCCAGC+TGG | 0.451237 | 2.1:+64910458 | None:intergenic |
| TTGAATAGCCCTACGCATAA+TGG | 0.455624 | 2.1:-64909829 | MS.gene27184:intron |
| CTTCTCAGTTTCAGAACGAC+TGG | 0.465661 | 2.1:+64909493 | None:intergenic |
| TGTTGAGCTAATGGAATTTG+TGG | 0.500110 | 2.1:-64910062 | MS.gene27184:CDS |
| ACTTGTCACCATCATCTAAC+GGG | 0.516413 | 2.1:+64910032 | None:intergenic |
| TCATCAAGACATAAGAACTT+AGG | 0.525555 | 2.1:-64909986 | MS.gene27184:intron |
| GGTGTAGCAGGGTAAACATA+AGG | 0.525917 | 2.1:+64910509 | None:intergenic |
| CATTTACCTCCATTATGCGT+AGG | 0.527451 | 2.1:+64909820 | None:intergenic |
| TTTGGCCAGCTGGCAAGAGA+AGG | 0.542150 | 2.1:-64910463 | MS.gene27184:CDS |
| AATCGTTGCTGCTCAACTTG+AGG | 0.547786 | 2.1:-64910217 | MS.gene27184:intron |
| ATTTACCTCCATTATGCGTA+GGG | 0.555661 | 2.1:+64909821 | None:intergenic |
| GAGATCAGCATACTGCCAAA+AGG | 0.559923 | 2.1:-64909715 | MS.gene27184:CDS |
| AGAAGCAACACCGGAAGCAA+AGG | 0.569743 | 2.1:-64910283 | MS.gene27184:CDS |
| AACTTGTCACCATCATCTAA+CGG | 0.580454 | 2.1:+64910031 | None:intergenic |
| CAGTGTAGGTGAAGAAATTG+TGG | 0.581309 | 2.1:+64910247 | None:intergenic |
| TAGAGCTGAAGATGATTCCA+TGG | 0.584821 | 2.1:+64910547 | None:intergenic |
| TGCAACAAAATGTTTGTACC+TGG | 0.588133 | 2.1:-64910413 | MS.gene27184:intron |
| CTCTAACACGAGGCTTATGA+GGG | 0.595924 | 2.1:-64909531 | MS.gene27184:CDS |
| TGTTGGTGCATGGTGTAGCA+GGG | 0.612247 | 2.1:+64910498 | None:intergenic |
| AGGATTCGGAGAAGCAACAC+CGG | 0.623746 | 2.1:-64910292 | MS.gene27184:CDS |
| GCTTATGAGGGATTACGTCT+CGG | 0.635882 | 2.1:-64909519 | MS.gene27184:CDS |
| GATAAACACCCGTTAGATGA+TGG | 0.636151 | 2.1:-64910040 | MS.gene27184:CDS |
| TGATGATTCTCTGAACATAG+TGG | 0.639406 | 2.1:+64910003 | None:intergenic |
| TGTAGGTGAAGAAATTGTGG+AGG | 0.663333 | 2.1:+64910250 | None:intergenic |
| AATAGCCCTACGCATAATGG+AGG | 0.664082 | 2.1:-64909826 | MS.gene27184:intron |
| TTTGTTGCATTGCAACCGTG+TGG | 0.685225 | 2.1:+64910427 | None:intergenic |
| GGAGCTCTCAATTATCCACA+CGG | 0.710162 | 2.1:-64910442 | MS.gene27184:CDS |
| TGGTCACTGACTCTAACACG+AGG | 0.758847 | 2.1:-64909541 | MS.gene27184:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGGTGTAATATTATATAT+AGG | - | chr2.1:64910296-64910315 | MS.gene27184:intron | 15.0% |
| !! | AAAACAAACTAAAACAACAA+AGG | + | chr2.1:64909863-64909882 | None:intergenic | 20.0% |
| !! | AATAGACACTTAACTAATAA+AGG | + | chr2.1:64910101-64910120 | None:intergenic | 20.0% |
| ! | AAAACAACAAAGGCAAATAA+AGG | + | chr2.1:64909853-64909872 | None:intergenic | 25.0% |
| ! | AACAGTTACATGCATTATTA+AGG | + | chr2.1:64910452-64910471 | None:intergenic | 25.0% |
| ! | ACACTTAACTAATAAAGGAT+TGG | + | chr2.1:64910096-64910115 | None:intergenic | 25.0% |
| ! | CACTTAACTAATAAAGGATT+GGG | + | chr2.1:64910095-64910114 | None:intergenic | 25.0% |
| ! | GGAAGTTGAATAAATTTAGT+AGG | + | chr2.1:64910431-64910450 | None:intergenic | 25.0% |
| ! | TAACCAACAACTATTCTATA+TGG | - | chr2.1:64910124-64910143 | MS.gene27184:intron | 25.0% |
| !! | AAAACTTGCTTAATAAGTGA+TGG | + | chr2.1:64910264-64910283 | None:intergenic | 25.0% |
| !! | TTACAACTTCAATTTTGTAC+TGG | - | chr2.1:64909650-64909669 | MS.gene27184:intron | 25.0% |
| !!! | AGTTTTGATTGAGTATACTA+TGG | - | chr2.1:64910278-64910297 | MS.gene27184:CDS | 25.0% |
| AAGACATAAGAACTTAGGTA+AGG | - | chr2.1:64910054-64910073 | MS.gene27184:CDS | 30.0% | |
| AATAGTGTGCTATGTTATCT+TGG | - | chr2.1:64909716-64909735 | MS.gene27184:CDS | 30.0% | |
| TCACCATATAGAATAGTTGT+TGG | + | chr2.1:64910130-64910149 | None:intergenic | 30.0% | |
| TCATCAAGACATAAGAACTT+AGG | - | chr2.1:64910049-64910068 | MS.gene27184:CDS | 30.0% | |
| !! | AACTTCAATTTTGTACTGGT+TGG | - | chr2.1:64909654-64909673 | MS.gene27184:intron | 30.0% |
| AAATCCTGTAAACTGATGTG+TGG | - | chr2.1:64910166-64910185 | MS.gene27184:intron | 35.0% | |
| AACTTGTCACCATCATCTAA+CGG | + | chr2.1:64910007-64910026 | None:intergenic | 35.0% | |
| ACAAAATGTTTGTACCTGGT+CGG | - | chr2.1:64909626-64909645 | MS.gene27184:intron | 35.0% | |
| ACAACAAAGGCAAATAAAGG+AGG | + | chr2.1:64909850-64909869 | None:intergenic | 35.0% | |
| ATTAGCTCAACATATGCTTC+AGG | + | chr2.1:64909965-64909984 | None:intergenic | 35.0% | |
| ATTTACCTCCATTATGCGTA+GGG | + | chr2.1:64910217-64910236 | None:intergenic | 35.0% | |
| CAACAAAGGCAAATAAAGGA+GGG | + | chr2.1:64909849-64909868 | None:intergenic | 35.0% | |
| TGAAGCATATGTTGAGCTAA+TGG | - | chr2.1:64909964-64909983 | MS.gene27184:intron | 35.0% | |
| TGATGATTCTCTGAACATAG+TGG | + | chr2.1:64910035-64910054 | None:intergenic | 35.0% | |
| TGCAACAAAATGTTTGTACC+TGG | - | chr2.1:64909622-64909641 | MS.gene27184:intron | 35.0% | |
| TGTTGAGCTAATGGAATTTG+TGG | - | chr2.1:64909973-64909992 | MS.gene27184:intron | 35.0% | |
| ! | ACTGCCAAAAGGATTTTGAA+TGG | - | chr2.1:64910331-64910350 | MS.gene27184:intron | 35.0% |
| ! | CCAAAAAGGATAGCTTTTGT+TGG | + | chr2.1:64909557-64909576 | None:intergenic | 35.0% |
| ! | CCAACAAAAGCTATCCTTTT+TGG | - | chr2.1:64909554-64909573 | MS.gene27184:intron | 35.0% |
| ! | CTGCCAAAAGGATTTTGAAT+GGG | - | chr2.1:64910332-64910351 | MS.gene27184:intron | 35.0% |
| ! | GAAGAAATTGTGGAGGTTTT+TGG | + | chr2.1:64909781-64909800 | None:intergenic | 35.0% |
| ! | TACGCATAATGGAGGTAAAT+GGG | - | chr2.1:64910217-64910236 | MS.gene27184:intron | 35.0% |
| ! | TGTCCCATTCAAAATCCTTT+TGG | + | chr2.1:64910338-64910357 | None:intergenic | 35.0% |
| !!! | AACGATTTTAACTGCAGTGT+AGG | + | chr2.1:64909805-64909824 | None:intergenic | 35.0% |
| !!! | CTTGAAGCGTTTAGCTTTTA+AGG | - | chr2.1:64910359-64910378 | MS.gene27184:intron | 35.0% |
| ACTTGTCACCATCATCTAAC+GGG | + | chr2.1:64910006-64910025 | None:intergenic | 40.0% | |
| ATTGAAGTTGTAAACCGACC+AGG | + | chr2.1:64909643-64909662 | None:intergenic | 40.0% | |
| CAGGATTATAACTCTGCTCA+TGG | + | chr2.1:64909946-64909965 | None:intergenic | 40.0% | |
| CAGTGTAGGTGAAGAAATTG+TGG | + | chr2.1:64909791-64909810 | None:intergenic | 40.0% | |
| CATTTACCTCCATTATGCGT+AGG | + | chr2.1:64910218-64910237 | None:intergenic | 40.0% | |
| GATAAACACCCGTTAGATGA+TGG | - | chr2.1:64909995-64910014 | MS.gene27184:CDS | 40.0% | |
| GTTATCTTGGATGCAGGATT+CGG | - | chr2.1:64909729-64909748 | MS.gene27184:intron | 40.0% | |
| TGCTATGTTATCTTGGATGC+AGG | - | chr2.1:64909723-64909742 | MS.gene27184:intron | 40.0% | |
| TGTAGGTGAAGAAATTGTGG+AGG | + | chr2.1:64909788-64909807 | None:intergenic | 40.0% | |
| TTGAATAGCCCTACGCATAA+TGG | - | chr2.1:64910206-64910225 | MS.gene27184:intron | 40.0% | |
| ! | CTACGCATAATGGAGGTAAA+TGG | - | chr2.1:64910216-64910235 | MS.gene27184:intron | 40.0% |
| AATAGCCCTACGCATAATGG+AGG | - | chr2.1:64910209-64910228 | MS.gene27184:intron | 45.0% | |
| AATCGTTGCTGCTCAACTTG+AGG | - | chr2.1:64909818-64909837 | MS.gene27184:intron | 45.0% | |
| ACTCTAACACGAGGCTTATG+AGG | - | chr2.1:64910503-64910522 | MS.gene27184:CDS | 45.0% | |
| AGAGCCACACATCAGTTTAC+AGG | + | chr2.1:64910173-64910192 | None:intergenic | 45.0% | |
| AGTCAGTGACCATCTACAAG+AGG | + | chr2.1:64910486-64910505 | None:intergenic | 45.0% | |
| CTCTAACACGAGGCTTATGA+GGG | - | chr2.1:64910504-64910523 | MS.gene27184:CDS | 45.0% | |
| CTTCTCAGTTTCAGAACGAC+TGG | + | chr2.1:64910545-64910564 | None:intergenic | 45.0% | |
| GAGATCAGCATACTGCCAAA+AGG | - | chr2.1:64910320-64910339 | MS.gene27184:intron | 45.0% | |
| GAGGTAAATGGGTGACTAGT+TGG | - | chr2.1:64910228-64910247 | MS.gene27184:CDS | 45.0% | |
| GCTTATGAGGGATTACGTCT+CGG | - | chr2.1:64910516-64910535 | MS.gene27184:CDS | 45.0% | |
| GGAGCTCTCAATTATCCACA+CGG | - | chr2.1:64909593-64909612 | MS.gene27184:intron | 45.0% | |
| GGTGTAGCAGGGTAAACATA+AGG | + | chr2.1:64909529-64909548 | None:intergenic | 45.0% | |
| TAGCGTTGTCCTCTTGTAGA+TGG | - | chr2.1:64910474-64910493 | MS.gene27184:CDS | 45.0% | |
| TTTGTTGCATTGCAACCGTG+TGG | + | chr2.1:64909611-64909630 | None:intergenic | 45.0% | |
| ! | GGATAGCTTTTGTTGGTGCA+TGG | + | chr2.1:64909550-64909569 | None:intergenic | 45.0% |
| !! | TTTTTGGCAGCCTTTGCTTC+CGG | + | chr2.1:64909765-64909784 | None:intergenic | 45.0% |
| AGAAGCAACACCGGAAGCAA+AGG | - | chr2.1:64909752-64909771 | MS.gene27184:intron | 50.0% | |
| AGGATTCGGAGAAGCAACAC+CGG | - | chr2.1:64909743-64909762 | MS.gene27184:intron | 50.0% | |
| TGTTGGTGCATGGTGTAGCA+GGG | + | chr2.1:64909540-64909559 | None:intergenic | 50.0% | |
| TTGTTGGTGCATGGTGTAGC+AGG | + | chr2.1:64909541-64909560 | None:intergenic | 50.0% | |
| !! | AGCTATCCTTTTTGGCCAGC+TGG | - | chr2.1:64909562-64909581 | MS.gene27184:intron | 50.0% |
| !! | TGGTCACTGACTCTAACACG+AGG | - | chr2.1:64910494-64910513 | MS.gene27184:CDS | 50.0% |
| CTCTTGCCAGCTGGCCAAAA+AGG | + | chr2.1:64909571-64909590 | None:intergenic | 55.0% | |
| TTTGGCCAGCTGGCAAGAGA+AGG | - | chr2.1:64909572-64909591 | MS.gene27184:intron | 55.0% | |
| !! | ACTGATGTGTGGCTCTGACG+TGG | - | chr2.1:64910177-64910196 | MS.gene27184:intron | 55.0% |
| GAGCTCCTTCTCTTGCCAGC+TGG | + | chr2.1:64909580-64909599 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 64909490 | 64910567 | 64909490 | ID=MS.gene27184 |
| chr2.1 | mRNA | 64909490 | 64910567 | 64909490 | ID=MS.gene27184.t1;Parent=MS.gene27184 |
| chr2.1 | exon | 64910414 | 64910567 | 64910414 | ID=MS.gene27184.t1.exon1;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64910414 | 64910567 | 64910414 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| chr2.1 | exon | 64910218 | 64910312 | 64910218 | ID=MS.gene27184.t1.exon2;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64910218 | 64910312 | 64910218 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| chr2.1 | exon | 64909987 | 64910104 | 64909987 | ID=MS.gene27184.t1.exon3;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64909987 | 64910104 | 64909987 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| chr2.1 | exon | 64909827 | 64909843 | 64909827 | ID=MS.gene27184.t1.exon4;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64909827 | 64909843 | 64909827 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| chr2.1 | exon | 64909677 | 64909739 | 64909677 | ID=MS.gene27184.t1.exon5;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64909677 | 64909739 | 64909677 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| chr2.1 | exon | 64909490 | 64909564 | 64909490 | ID=MS.gene27184.t1.exon6;Parent=MS.gene27184.t1 |
| chr2.1 | CDS | 64909490 | 64909564 | 64909490 | ID=cds.MS.gene27184.t1;Parent=MS.gene27184.t1 |
| Gene Sequence |
| Protein sequence |
