Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28413.t1 | XP_003630233.1 | 98.1 | 430 | 8 | 0 | 1 | 430 | 1 | 430 | 5.90E-243 | 850.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28413.t1 | P94479 | 43.8 | 372 | 207 | 2 | 55 | 426 | 27 | 396 | 6.9e-91 | 337.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28413.t1 | G7LD70 | 98.1 | 430 | 8 | 0 | 1 | 430 | 1 | 430 | 4.3e-243 | 850.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene28413 | 0.826055 | 3.35E-54 | -1.69E-46 |
| MS.gene058019 | MS.gene28413 | 0.802443 | 5.60E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene28413 | 0.818906 | 1.53E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28413.t1 | MTR_8g093250 | 98.140 | 430 | 8 | 0 | 1 | 430 | 1 | 430 | 0.0 | 871 |
| MS.gene28413.t1 | MTR_5g053650 | 50.943 | 318 | 134 | 4 | 626 | 929 | 1 | 310 | 3.12e-99 | 317 |
| MS.gene28413.t1 | MTR_3g048390 | 30.585 | 376 | 231 | 7 | 487 | 838 | 20 | 389 | 1.60e-46 | 174 |
| MS.gene28413.t1 | MTR_6g048830 | 38.636 | 220 | 125 | 6 | 633 | 846 | 45 | 260 | 1.33e-34 | 134 |
| MS.gene28413.t1 | MTR_0073s0040 | 33.772 | 228 | 145 | 3 | 624 | 847 | 54 | 279 | 2.89e-33 | 132 |
| MS.gene28413.t1 | MTR_0031s0120 | 31.679 | 262 | 150 | 8 | 617 | 867 | 141 | 384 | 2.18e-31 | 127 |
| MS.gene28413.t1 | MTR_7g083690 | 29.619 | 341 | 201 | 11 | 630 | 933 | 177 | 515 | 8.91e-31 | 131 |
| MS.gene28413.t1 | MTR_1148s0010 | 32.710 | 214 | 137 | 2 | 630 | 837 | 141 | 353 | 1.30e-30 | 129 |
| MS.gene28413.t1 | MTR_4g005250 | 41.379 | 145 | 78 | 4 | 649 | 792 | 222 | 360 | 2.15e-29 | 121 |
| MS.gene28413.t1 | MTR_7g057470 | 55.660 | 106 | 46 | 1 | 779 | 884 | 1 | 105 | 3.67e-28 | 122 |
| MS.gene28413.t1 | MTR_7g057490 | 55.660 | 106 | 46 | 1 | 779 | 884 | 1 | 105 | 3.67e-28 | 122 |
| MS.gene28413.t1 | MTR_8g045280 | 36.066 | 183 | 115 | 2 | 623 | 804 | 365 | 546 | 5.37e-28 | 122 |
| MS.gene28413.t1 | MTR_2g060030 | 40.476 | 168 | 91 | 3 | 635 | 800 | 336 | 496 | 1.49e-27 | 120 |
| MS.gene28413.t1 | MTR_6g054900 | 36.111 | 180 | 108 | 3 | 619 | 798 | 25 | 197 | 9.72e-27 | 111 |
| MS.gene28413.t1 | MTR_5g073130 | 32.642 | 193 | 126 | 3 | 649 | 838 | 173 | 364 | 3.04e-26 | 112 |
| MS.gene28413.t1 | MTR_4g027930 | 32.967 | 182 | 106 | 2 | 624 | 805 | 1 | 166 | 1.85e-25 | 106 |
| MS.gene28413.t1 | MTR_6g053340 | 46.226 | 106 | 56 | 1 | 687 | 792 | 152 | 256 | 1.35e-24 | 105 |
| MS.gene28413.t1 | MTR_5g048260 | 41.045 | 134 | 76 | 3 | 628 | 760 | 104 | 235 | 1.38e-23 | 100 |
| MS.gene28413.t1 | MTR_4g061490 | 43.200 | 125 | 70 | 1 | 660 | 784 | 306 | 429 | 8.16e-23 | 104 |
| MS.gene28413.t1 | MTR_0062s0050 | 41.104 | 163 | 82 | 3 | 757 | 905 | 1 | 163 | 1.67e-22 | 103 |
| MS.gene28413.t1 | MTR_0062s0080 | 41.104 | 163 | 82 | 3 | 757 | 905 | 1 | 163 | 1.81e-22 | 103 |
| MS.gene28413.t1 | MTR_7g108760 | 41.739 | 115 | 66 | 1 | 687 | 801 | 51 | 164 | 9.96e-22 | 100 |
| MS.gene28413.t1 | MTR_8g058470 | 43.396 | 106 | 59 | 1 | 687 | 792 | 51 | 155 | 1.00e-21 | 100 |
| MS.gene28413.t1 | MTR_0242s0010 | 27.111 | 225 | 117 | 3 | 617 | 835 | 307 | 490 | 9.40e-21 | 97.8 |
| MS.gene28413.t1 | MTR_6g024400 | 26.603 | 312 | 176 | 8 | 698 | 960 | 312 | 619 | 1.05e-20 | 98.2 |
| MS.gene28413.t1 | MTR_8g103960 | 40.336 | 119 | 70 | 1 | 705 | 823 | 3 | 120 | 1.14e-20 | 89.7 |
| MS.gene28413.t1 | MTR_7g033825 | 24.907 | 269 | 146 | 6 | 623 | 891 | 32 | 244 | 6.48e-17 | 86.3 |
| MS.gene28413.t1 | MTR_2g103400 | 29.630 | 162 | 106 | 3 | 664 | 824 | 139 | 293 | 1.40e-15 | 81.3 |
| MS.gene28413.t1 | MTR_3g049960 | 32.886 | 149 | 70 | 1 | 625 | 773 | 192 | 310 | 1.94e-15 | 81.3 |
| MS.gene28413.t1 | MTR_5g065250 | 30.000 | 140 | 97 | 1 | 652 | 791 | 203 | 341 | 3.52e-15 | 80.5 |
| MS.gene28413.t1 | MTR_5g054740 | 27.612 | 268 | 148 | 10 | 705 | 946 | 488 | 735 | 1.27e-13 | 75.9 |
| MS.gene28413.t1 | MTR_1g056270 | 67.391 | 46 | 15 | 0 | 757 | 802 | 1 | 46 | 1.93e-12 | 68.2 |
| MS.gene28413.t1 | MTR_6g039210 | 30.102 | 196 | 109 | 4 | 514 | 703 | 240 | 413 | 3.92e-12 | 69.7 |
| MS.gene28413.t1 | MTR_3g022785 | 25.121 | 207 | 105 | 2 | 606 | 812 | 292 | 448 | 4.33e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 269 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGTTCCCTTCAAGATTT+TGG | 0.069730 | 4.4:+8881910 | MS.gene28413:CDS |
| CGCCTTTCTGCGCCCGGGCT+TGG | 0.206997 | 4.4:+8882959 | MS.gene28413:CDS |
| TGCTGAAGTTCTGGCATCTA+AGG | 0.222981 | 4.4:+8883167 | MS.gene28413:CDS |
| CTTCCTGATTGCTGAAGTTC+TGG | 0.223414 | 4.4:+8883158 | MS.gene28413:CDS |
| CGTCATTCTGTTGAGGTTAA+AGG | 0.229958 | 4.4:+8886160 | MS.gene28413:CDS |
| ATCCACAAATCTATCATATT+TGG | 0.236424 | 4.4:-8885120 | None:intergenic |
| ACATTCTCGGTAATAGATTT+AGG | 0.236564 | 4.4:-8886268 | None:intergenic |
| AAAAGGGACTCTGGTGGTTT+GGG | 0.240265 | 4.4:+8881892 | MS.gene28413:CDS |
| GACACATTAGAGGAAGTAAT+TGG | 0.241899 | 4.4:+8881868 | MS.gene28413:CDS |
| TTGGGCTAACAAATGTATTA+TGG | 0.246237 | 4.4:+8885541 | MS.gene28413:CDS |
| GAAAAGGGACTCTGGTGGTT+TGG | 0.264666 | 4.4:+8881891 | MS.gene28413:CDS |
| AGACTCAACACAGTATTTCT+TGG | 0.267277 | 4.4:+8890659 | MS.gene28413:CDS |
| AACTCGGCGAAGGACTTTAT+TGG | 0.270899 | 4.4:+8885707 | MS.gene28413:CDS |
| TGCAATGTGTCTCTTTCTTA+TGG | 0.272210 | 4.4:+8884344 | MS.gene28413:CDS |
| GGTGCATGGGTGAGGTGCTA+TGG | 0.295822 | 4.4:+8885788 | MS.gene28413:CDS |
| GTTTGTCAGCCGAAGCTTTA+CGG | 0.299636 | 4.4:+8884562 | MS.gene28413:CDS |
| CGCTTCATGAGGATGTTGTT+TGG | 0.310309 | 4.4:+8885522 | MS.gene28413:CDS |
| TTTGGTGGTTGCACTGGTTA+TGG | 0.310624 | 4.4:+8881069 | MS.gene28413:CDS |
| TGATATCTTCTTCTTAGAAT+TGG | 0.312540 | 4.4:+8885832 | MS.gene28413:CDS |
| TGTGAGGAGGGATTGGCTTC+TGG | 0.314783 | 4.4:+8885458 | MS.gene28413:CDS |
| TTCTTTCACCTCAAATGGTT+GGG | 0.316414 | 4.4:+8883038 | MS.gene28413:CDS |
| TTCATCAAATGCAAGATGAT+TGG | 0.326224 | 4.4:+8886134 | MS.gene28413:CDS |
| GTGCATGGGTGAGGTGCTAT+GGG | 0.331540 | 4.4:+8885789 | MS.gene28413:CDS |
| TTTATAGAAGACTTGGAGTT+TGG | 0.343431 | 4.4:+8886007 | MS.gene28413:CDS |
| GGATTTCAAGCTAATTCCTA+TGG | 0.353452 | 4.4:+8885628 | MS.gene28413:CDS |
| TTGCCACAGGGCCCTTTGCT+AGG | 0.355881 | 4.4:+8884524 | MS.gene28413:intron |
| CCATGTGGCGGATATGTTGC+TGG | 0.367862 | 4.4:+8882905 | MS.gene28413:CDS |
| GCCGATGGAACCTTTATTGA+TGG | 0.368807 | 4.4:+8883711 | MS.gene28413:CDS |
| GGAGGAGTGTTCTAAGTCTA+AGG | 0.373996 | 4.4:+8886225 | MS.gene28413:CDS |
| TTCGTGGCACCATCTGTAAT+TGG | 0.374550 | 4.4:+8886322 | MS.gene28413:CDS |
| GCTTCATGAGGATGTTGTTT+GGG | 0.378997 | 4.4:+8885523 | MS.gene28413:CDS |
| ATTATTTCTTTCACCTCAAA+TGG | 0.379919 | 4.4:+8883033 | MS.gene28413:CDS |
| GAGGGATTGGCTTCTGGTTG+TGG | 0.380017 | 4.4:+8885464 | MS.gene28413:CDS |
| CCGAGGATGGTGGACTTGAC+TGG | 0.385164 | 4.4:+8882035 | MS.gene28413:CDS |
| GCTGCGCGCCTTTCTGCGCC+CGG | 0.386088 | 4.4:+8882953 | MS.gene28413:CDS |
| CCAATTGCGGGTACCACTCC+TGG | 0.392343 | 4.4:+8883535 | MS.gene28413:CDS |
| TTGACAGAAATGCTTTGATC+AGG | 0.392543 | 4.4:+8886374 | MS.gene28413:CDS |
| TCTTCTATGATTGCCGTCCT+TGG | 0.397557 | 4.4:+8885735 | MS.gene28413:CDS |
| ACTTCCTCTAATGTGTCATA+TGG | 0.402174 | 4.4:-8881862 | None:intergenic |
| TGAAAAGCAGGTCACCAAAA+AGG | 0.403786 | 4.4:+8886296 | MS.gene28413:CDS |
| ACTAGTACTTCCATCAATAA+AGG | 0.405469 | 4.4:-8883721 | None:intergenic |
| AAATGCAGGGTGGCACTCAT+TGG | 0.408594 | 4.4:+8883875 | MS.gene28413:intron |
| ATTGCTTCCCCAACCATTTG+AGG | 0.410807 | 4.4:-8883046 | None:intergenic |
| TCATCAAATGCAAGATGATT+GGG | 0.417110 | 4.4:+8886135 | MS.gene28413:CDS |
| AGTTGGATAGAGAGAGGAAG+AGG | 0.423629 | 4.4:+8885321 | MS.gene28413:CDS |
| TCGGCAGGGTGCAGATTTGA+TGG | 0.424522 | 4.4:+8882844 | MS.gene28413:intron |
| ACCACAATCCACACCAAGCC+CGG | 0.425545 | 4.4:-8882972 | None:intergenic |
| TAGAGGAAGTAATTGGGAAA+AGG | 0.426036 | 4.4:+8881875 | MS.gene28413:CDS |
| CTTCACGACATCCTCCTTCC+TGG | 0.433891 | 4.4:-8885390 | None:intergenic |
| CAGCACTTTGGTGGTTGCAC+TGG | 0.435214 | 4.4:+8881063 | MS.gene28413:intron |
| TGGCACTCATTGGACTCAAT+GGG | 0.438629 | 4.4:+8883885 | MS.gene28413:CDS |
| AGAGGTCATGTGGTTATTCA+TGG | 0.439010 | 4.4:+8882107 | MS.gene28413:CDS |
| TAGGTAAGTGGCTCACTCTT+AGG | 0.439547 | 4.4:-8884590 | None:intergenic |
| AGTGGTACCCGCAATTGGTT+TGG | 0.441177 | 4.4:-8883530 | None:intergenic |
| ATCAGCTATTGTTCGTTCAC+AGG | 0.442751 | 4.4:+8881155 | MS.gene28413:CDS |
| GTGGCACTCATTGGACTCAA+TGG | 0.444697 | 4.4:+8883884 | MS.gene28413:CDS |
| AACCTGAACTGTATCACATC+GGG | 0.445399 | 4.4:-8883220 | None:intergenic |
| ATGCCAGAACTTCAGCAATC+AGG | 0.447778 | 4.4:-8883161 | None:intergenic |
| ACTTGTCAACAGTTCTTCTC+AGG | 0.450400 | 4.4:+8881638 | MS.gene28413:intron |
| GGTGAAATACCGAGAAGTTC+CGG | 0.451794 | 4.4:+8881933 | MS.gene28413:CDS |
| TCTTTGTTGTGAGGAGGGAT+TGG | 0.452068 | 4.4:+8885451 | MS.gene28413:CDS |
| CTGCGCGCCTTTCTGCGCCC+GGG | 0.452242 | 4.4:+8882954 | MS.gene28413:CDS |
| CCAGGAGTGGTACCCGCAAT+TGG | 0.455539 | 4.4:-8883535 | None:intergenic |
| AGAGGAAGTAATTGGGAAAA+GGG | 0.458354 | 4.4:+8881876 | MS.gene28413:CDS |
| TAGGTTCCACCGTAAAGCTT+CGG | 0.459754 | 4.4:-8884571 | None:intergenic |
| GCCTGGCAAAATACAGCAAA+TGG | 0.460230 | 4.4:-8883774 | None:intergenic |
| CTGTATGGATTTATTTAACT+CGG | 0.463566 | 4.4:+8885691 | MS.gene28413:CDS |
| TTTCTTTCACCTCAAATGGT+TGG | 0.466714 | 4.4:+8883037 | MS.gene28413:CDS |
| TCATACACCAAACCAATTGC+GGG | 0.468084 | 4.4:+8883523 | MS.gene28413:CDS |
| GGCTTATGAGAGGGGTGCAT+GGG | 0.471101 | 4.4:+8885775 | MS.gene28413:CDS |
| TTCTGCGCCCGGGCTTGGTG+TGG | 0.471332 | 4.4:+8882964 | MS.gene28413:CDS |
| ATGAGAGGGGTGCATGGGTG+AGG | 0.473422 | 4.4:+8885780 | MS.gene28413:CDS |
| TTATTTGCCCTCTTGAGGCC+AGG | 0.474037 | 4.4:+8881683 | MS.gene28413:CDS |
| GTTCAAGTGGTAAAGGAGGC+CGG | 0.474687 | 4.4:+8885419 | MS.gene28413:CDS |
| GTTGGATAGAGAGAGGAAGA+GGG | 0.476113 | 4.4:+8885322 | MS.gene28413:CDS |
| CAGACCTCTGATGCATATCC+AGG | 0.477506 | 4.4:-8883553 | None:intergenic |
| TCTGGAACGAAGGGATTGAT+AGG | 0.478948 | 4.4:-8880131 | None:intergenic |
| AGTAGGTGTGGGTAAGAAAG+TGG | 0.482211 | 4.4:+8885364 | MS.gene28413:CDS |
| TACTGTGAATAAAGATCGTA+TGG | 0.483714 | 4.4:+8885892 | MS.gene28413:CDS |
| TGCCTGTAGGCCGTACAACT+TGG | 0.486168 | 4.4:+8883442 | MS.gene28413:intron |
| AGGATGTGGATGGTGTTAAG+AGG | 0.487279 | 4.4:+8885168 | MS.gene28413:CDS |
| GTTGGAGGTTCAAGTGGTAA+AGG | 0.487860 | 4.4:+8885412 | MS.gene28413:CDS |
| CACCAAGCCCGGGCGCAGAA+AGG | 0.488183 | 4.4:-8882961 | None:intergenic |
| AGTTTGAGTGTTAATGACAT+AGG | 0.488359 | 4.4:+8882136 | MS.gene28413:CDS |
| TGGATAGAGAGAGGAAGAGG+GGG | 0.488375 | 4.4:+8885324 | MS.gene28413:CDS |
| TGGCTTATGAGAGGGGTGCA+TGG | 0.489779 | 4.4:+8885774 | MS.gene28413:CDS |
| CCGAATCTTTGTTGTGAGGA+GGG | 0.490261 | 4.4:+8885446 | MS.gene28413:CDS |
| CCCTCCTCACAACAAAGATT+CGG | 0.490490 | 4.4:-8885446 | None:intergenic |
| TAGCGAATGGTAGATGTTGT+TGG | 0.491205 | 4.4:-8880089 | None:intergenic |
| TGCTCCATATGACACATTAG+AGG | 0.492829 | 4.4:+8881858 | MS.gene28413:CDS |
| GTGCTTTATTTGCCCTCTTG+AGG | 0.493644 | 4.4:+8881678 | MS.gene28413:CDS |
| TGCATGGGTGAGGTGCTATG+GGG | 0.493671 | 4.4:+8885790 | MS.gene28413:CDS |
| CAGGGTGCAGATTTGATGGC+AGG | 0.495304 | 4.4:+8882848 | MS.gene28413:intron |
| AACAGAGTCTCTTTGTCAGC+AGG | 0.496025 | 4.4:+8886484 | MS.gene28413:CDS |
| ATATCATTCCAAGCATGTAT+TGG | 0.497248 | 4.4:-8885815 | None:intergenic |
| GATGCAAAATCCTGAGTGTT+CGG | 0.498240 | 4.4:+8882695 | MS.gene28413:intron |
| AAGTCTTCTATAAAACGGAT+TGG | 0.500419 | 4.4:-8885998 | None:intergenic |
| TTGGATAGAGAGAGGAAGAG+GGG | 0.500469 | 4.4:+8885323 | MS.gene28413:CDS |
| TGGGTAAGAAAGTGGTGGCC+AGG | 0.500500 | 4.4:+8885372 | MS.gene28413:CDS |
| TCATGGTGGATAACTGCTAT+GGG | 0.500774 | 4.4:+8882718 | MS.gene28413:CDS |
| GAAGGAGGATGTCGTGAAGT+TGG | 0.501276 | 4.4:+8885394 | MS.gene28413:CDS |
| ATTTCACCCCAAAATCTTGA+AGG | 0.501972 | 4.4:-8881918 | None:intergenic |
| AATTAATCTACCTCGGATTG+AGG | 0.502809 | 4.4:-8885855 | None:intergenic |
| GGTTATGGTCATGATGAAGC+TGG | 0.503942 | 4.4:+8881084 | MS.gene28413:CDS |
| AGTCTTCTATAAAACGGATT+GGG | 0.506877 | 4.4:-8885997 | None:intergenic |
| TTTCATCAGCTTGCCGAGGA+TGG | 0.507265 | 4.4:+8882022 | MS.gene28413:intron |
| GATGAACAAGAGCATCAACC+AGG | 0.508506 | 4.4:-8886117 | None:intergenic |
| AATGGTTGGGGAAGCAATCA+AGG | 0.508889 | 4.4:+8883051 | MS.gene28413:CDS |
| TGACAAACGAAGGGTCTGCT+TGG | 0.509294 | 4.4:-8884547 | None:intergenic |
| TGTTCAGAGAAGCTCTCCTG+TGG | 0.510171 | 4.4:+8883498 | MS.gene28413:CDS |
| ACTCCAAGTCTTCTATAAAA+CGG | 0.511192 | 4.4:-8886003 | None:intergenic |
| AGCATGTAGTGTTGAAGGTA+AGG | 0.512216 | 4.4:+8885148 | MS.gene28413:CDS |
| CCTTAGAATGTTCGCAAATG+TGG | 0.512701 | 4.4:+8885097 | MS.gene28413:intron |
| TAATGCTGTCAGCACTTTGG+TGG | 0.513811 | 4.4:+8881054 | MS.gene28413:intron |
| GAGGCAGCATGTAGTGTTGA+AGG | 0.514153 | 4.4:+8885143 | MS.gene28413:CDS |
| CATCAGCTTGCCGAGGATGG+TGG | 0.516636 | 4.4:+8882025 | MS.gene28413:intron |
| GTCATGGTGGATAACTGCTA+TGG | 0.517473 | 4.4:+8882717 | MS.gene28413:CDS |
| TAAGAAAGTGGTGGCCAGGA+AGG | 0.521411 | 4.4:+8885376 | MS.gene28413:CDS |
| TAGATTTCAGAACTTCTCCT+AGG | 0.521668 | 4.4:-8883915 | None:intergenic |
| GTTTGAGTGTTAATGACATA+GGG | 0.522137 | 4.4:+8882137 | MS.gene28413:CDS |
| GTTCAGAGAAGCTCTCCTGT+GGG | 0.522769 | 4.4:+8883499 | MS.gene28413:CDS |
| GATTTCAAGCTAATTCCTAT+GGG | 0.523147 | 4.4:+8885629 | MS.gene28413:CDS |
| AGCGAGTACCTCTGGAACGA+AGG | 0.525027 | 4.4:-8880141 | None:intergenic |
| TTCAAGTGGTAAAGGAGGCC+GGG | 0.525224 | 4.4:+8885420 | MS.gene28413:CDS |
| GTTAACTTGTGCTGAAAGAA+TGG | 0.526680 | 4.4:+8884366 | MS.gene28413:CDS |
| ACACATTAGAGGAAGTAATT+GGG | 0.527457 | 4.4:+8881869 | MS.gene28413:CDS |
| CCAGTCAAGTCCACCATCCT+CGG | 0.529194 | 4.4:-8882035 | None:intergenic |
| GGGTCTGCTTGGCCTAGCAA+AGG | 0.529757 | 4.4:-8884536 | None:intergenic |
| TCTCAGGTTATCTTTGCCGA+TGG | 0.530646 | 4.4:+8883696 | MS.gene28413:intron |
| TCTTTCACCTCAAATGGTTG+GGG | 0.531494 | 4.4:+8883039 | MS.gene28413:CDS |
| AAAGGCTCTCATGATATCAC+CGG | 0.531537 | 4.4:-8883002 | None:intergenic |
| CCCGGGCTTGGTGTGGATTG+TGG | 0.531980 | 4.4:+8882971 | MS.gene28413:CDS |
| CCGGAAGTTGGAGTTAGAAA+AGG | 0.533511 | 4.4:+8885274 | MS.gene28413:CDS |
| TAAGTCTCAATGTTCAAGTC+CGG | 0.533715 | 4.4:+8886072 | MS.gene28413:CDS |
| ACTCAATGGGGACTAGTCCT+AGG | 0.534013 | 4.4:+8883898 | MS.gene28413:CDS |
| GTGGATTGTGGTTCAACTCC+CGG | 0.534401 | 4.4:+8882983 | MS.gene28413:CDS |
| TTAGAAAATATGCAAGAGAA+AGG | 0.534636 | 4.4:+8890624 | MS.gene28413:intron |
| CAACAATTTCTGCAAAAGCC+TGG | 0.536674 | 4.4:-8881125 | None:intergenic |
| GTAACGGCGGGAGAGGAAGT+AGG | 0.538604 | 4.4:+8885347 | MS.gene28413:CDS |
| AGCCACTTACCTACTCCGTC+AGG | 0.542343 | 4.4:+8884600 | MS.gene28413:CDS |
| ACAACAAAGATTCGGCGTCC+CGG | 0.542448 | 4.4:-8885438 | None:intergenic |
| TAGTGTTGAAGGTAAGGATG+TGG | 0.542461 | 4.4:+8885154 | MS.gene28413:CDS |
| CAAAATGTGCACTCATACAG+AGG | 0.542723 | 4.4:+8882089 | MS.gene28413:CDS |
| AGTAGCATACCGGAACTTCT+CGG | 0.544340 | 4.4:-8881942 | None:intergenic |
| GAGATTCGAACGGGATGCGT+AGG | 0.544625 | 4.4:-8880062 | None:intergenic |
| AGAATGTTGTTGTGAAAAGC+AGG | 0.546875 | 4.4:+8886284 | MS.gene28413:CDS |
| GCTCTCCCCTCCAACTCCCT+CGG | 0.547931 | 4.4:-8885233 | None:intergenic |
| GTCATACACCAAACCAATTG+CGG | 0.548656 | 4.4:+8883522 | MS.gene28413:CDS |
| CCTCTTGAGGCCAGGAGATG+AGG | 0.549373 | 4.4:+8881691 | MS.gene28413:CDS |
| CCACAATCCACACCAAGCCC+GGG | 0.549428 | 4.4:-8882971 | None:intergenic |
| GCTGAAGTTCTGGCATCTAA+GGG | 0.550000 | 4.4:+8883168 | MS.gene28413:CDS |
| TGTTAATGCTGTCAGCACTT+TGG | 0.550318 | 4.4:+8881051 | MS.gene28413:intron |
| GGTCTGCTTGGCCTAGCAAA+GGG | 0.550955 | 4.4:-8884535 | None:intergenic |
| TGCAGCTATCCGTAGTGAGT+TGG | 0.551744 | 4.4:+8885304 | MS.gene28413:CDS |
| GCCGAATCTTTGTTGTGAGG+AGG | 0.552866 | 4.4:+8885445 | MS.gene28413:CDS |
| AAAAGGGTTACCTCATCTCC+TGG | 0.553149 | 4.4:-8881701 | None:intergenic |
| GTGTGTCGAAGGTGTCTGTA+TGG | 0.554061 | 4.4:+8885676 | MS.gene28413:CDS |
| ATGGGGTGCCAATACATGCT+TGG | 0.558269 | 4.4:+8885807 | MS.gene28413:CDS |
| GTTATGGTCATGATGAAGCT+GGG | 0.559133 | 4.4:+8881085 | MS.gene28413:CDS |
| ACTAGTGAGCTTTCATGTGA+TGG | 0.559350 | 4.4:+8883738 | MS.gene28413:CDS |
| CAGTTGAGAGTAATGATAGC+CGG | 0.559686 | 4.4:+8885255 | MS.gene28413:CDS |
| TTCTGAATTCAATGCATCAA+CGG | 0.560614 | 4.4:-8880246 | None:intergenic |
| GAGTAATGATAGCCGGAAGT+TGG | 0.561213 | 4.4:+8885262 | MS.gene28413:CDS |
| CCTCATCTCCTGGCCTCAAG+AGG | 0.563110 | 4.4:-8881691 | None:intergenic |
| TTGGGTTCGTCATTCTGTTG+AGG | 0.563407 | 4.4:+8886153 | MS.gene28413:CDS |
| GTAATTGGGAAAAGGGACTC+TGG | 0.564622 | 4.4:+8881883 | MS.gene28413:CDS |
| GGCAGTTTGATAAAGAATCC+TGG | 0.564656 | 4.4:+8882869 | MS.gene28413:CDS |
| CACCTGACGGAGTAGGTAAG+TGG | 0.564794 | 4.4:-8884602 | None:intergenic |
| GGCAAATAAAGCACATGTGA+TGG | 0.565843 | 4.4:-8881669 | None:intergenic |
| TGGTAATGTTAGAACAGAGA+TGG | 0.566607 | 4.4:+8885484 | MS.gene28413:CDS |
| TCCATCAATAAAGGTTCCAT+CGG | 0.567010 | 4.4:-8883712 | None:intergenic |
| GGCCAAATATGATAGATTTG+TGG | 0.570537 | 4.4:+8885118 | MS.gene28413:CDS |
| CTCTAAAAGAAAATTAGTGA+AGG | 0.570986 | 4.4:+8886417 | MS.gene28413:CDS |
| GGATAGAGAGAGGAAGAGGG+GGG | 0.571428 | 4.4:+8885325 | MS.gene28413:CDS |
| TTTGCAAGAAGAGGAACCCC+TGG | 0.572437 | 4.4:+8886099 | MS.gene28413:CDS |
| ATATGATAGATTTGTGGATG+AGG | 0.573112 | 4.4:+8885124 | MS.gene28413:CDS |
| TGATGCAAAACCAACATGCC+TGG | 0.574370 | 4.4:-8883791 | None:intergenic |
| ACAGCAAATGGCTCTCTAAG+AGG | 0.576106 | 4.4:-8883762 | None:intergenic |
| ATATTATCGCATACCACCAT+GGG | 0.576389 | 4.4:-8882765 | None:intergenic |
| GTTGGAATGGAGATTCGAAC+GGG | 0.578637 | 4.4:-8880071 | None:intergenic |
| CATATTATCGCATACCACCA+TGG | 0.578930 | 4.4:-8882766 | None:intergenic |
| AAGCTTCGGCTGACAAACGA+AGG | 0.578982 | 4.4:-8884557 | None:intergenic |
| TTTCACCCCAAAATCTTGAA+GGG | 0.581207 | 4.4:-8881917 | None:intergenic |
| GGATTTATTTAACTCGGCGA+AGG | 0.583217 | 4.4:+8885697 | MS.gene28413:CDS |
| GAAAGTGGTGGCCAGGAAGG+AGG | 0.583828 | 4.4:+8885379 | MS.gene28413:CDS |
| TAACTGCTATGGGGAACTTG+TGG | 0.585753 | 4.4:+8882728 | MS.gene28413:CDS |
| TGACAGAAATGCTTTGATCA+GGG | 0.585973 | 4.4:+8886375 | MS.gene28413:CDS |
| AATGGTAGATGTTGTTGGAA+TGG | 0.587350 | 4.4:-8880084 | None:intergenic |
| GTTGGAGTTAGAAAAGGTGA+AGG | 0.591035 | 4.4:+8885280 | MS.gene28413:CDS |
| GGGACGTGAAGCTCTTGACC+AGG | 0.591339 | 4.4:+8881107 | MS.gene28413:CDS |
| TCCTCTTCTTGCAAACACGC+CGG | 0.591614 | 4.4:-8886091 | None:intergenic |
| CTCACGGCTTCCAAGTTGTA+CGG | 0.592315 | 4.4:-8883452 | None:intergenic |
| CATGGAGCAATCGTTCCACC+AGG | 0.592431 | 4.4:-8882887 | None:intergenic |
| GATGGTGTTAAGAGGAGTGA+GGG | 0.595328 | 4.4:+8885176 | MS.gene28413:CDS |
| GTCACATTCAATTAACCGCA+AGG | 0.595751 | 4.4:+8886204 | MS.gene28413:CDS |
| GCACCCCTCTCATAAGCCAC+AGG | 0.597411 | 4.4:-8885770 | None:intergenic |
| CACTCCTGGATATGCATCAG+AGG | 0.597649 | 4.4:+8883549 | MS.gene28413:CDS |
| CCAGCAACATATCCGCCACA+TGG | 0.599034 | 4.4:-8882905 | None:intergenic |
| TCTAAAAGAAAATTAGTGAA+GGG | 0.600055 | 4.4:+8886418 | MS.gene28413:CDS |
| GTGAAAAGCTTTAAGAACAC+GGG | 0.600306 | 4.4:-8880306 | None:intergenic |
| TGTTGGAATGGAGATTCGAA+CGG | 0.600412 | 4.4:-8880072 | None:intergenic |
| TATCAATCCCTTCGTTCCAG+AGG | 0.600711 | 4.4:+8880133 | MS.gene28413:CDS |
| TTCAAGCTAATTCCTATGGG+TGG | 0.602066 | 4.4:+8885632 | MS.gene28413:CDS |
| ACAGGTGCATCTTTCGTCCA+AGG | 0.603688 | 4.4:-8885752 | None:intergenic |
| CCAGCTTCACCTGACGGAGT+AGG | 0.604246 | 4.4:-8884609 | None:intergenic |
| AAATCCTGAGTGTTCGGTCA+TGG | 0.605022 | 4.4:+8882701 | MS.gene28413:CDS |
| ATGCACCTGTGGCTTATGAG+AGG | 0.605239 | 4.4:+8885765 | MS.gene28413:CDS |
| TACAGACACCTTCGACACAC+GGG | 0.605533 | 4.4:-8885673 | None:intergenic |
| TTATTGCACCCGTGTGTCGA+AGG | 0.605983 | 4.4:+8885665 | MS.gene28413:CDS |
| GGAGGATGTCGTGAAGTTGG+AGG | 0.605998 | 4.4:+8885397 | MS.gene28413:CDS |
| GGCGGGAGAGGAAGTAGGTG+TGG | 0.606761 | 4.4:+8885352 | MS.gene28413:CDS |
| CATGTGGCGGATATGTTGCT+GGG | 0.607387 | 4.4:+8882906 | MS.gene28413:CDS |
| CGTGAAGTTGGAGGTTCAAG+TGG | 0.607488 | 4.4:+8885406 | MS.gene28413:CDS |
| TTAGAATTGGCCTCAATCCG+AGG | 0.607988 | 4.4:+8885845 | MS.gene28413:CDS |
| GAATTCAGAATTCAGAGCAG+TGG | 0.610064 | 4.4:+8880259 | MS.gene28413:CDS |
| TCTCTCTATCCAACTCACTA+CGG | 0.610711 | 4.4:-8885313 | None:intergenic |
| ACAGTATTTACCGCTTCATG+AGG | 0.610828 | 4.4:+8885511 | MS.gene28413:CDS |
| GAAGCGAGAGGCATAGCGAA+TGG | 0.611375 | 4.4:-8880102 | None:intergenic |
| TCCGGCGTGTTTGCAAGAAG+AGG | 0.611913 | 4.4:+8886090 | MS.gene28413:CDS |
| ATACAGACACCTTCGACACA+CGG | 0.612577 | 4.4:-8885674 | None:intergenic |
| GTAGTGAGTTGGATAGAGAG+AGG | 0.614233 | 4.4:+8885315 | MS.gene28413:CDS |
| TCCTGAGTGTTCGGTCATGG+TGG | 0.614793 | 4.4:+8882704 | MS.gene28413:CDS |
| TGCTATGGGGAACTTGTGGA+AGG | 0.615123 | 4.4:+8882732 | MS.gene28413:CDS |
| AGGATAAGCAGAGAAGGCGC+AGG | 0.615839 | 4.4:-8880042 | None:intergenic |
| CTCATCTCCTGGCCTCAAGA+GGG | 0.618882 | 4.4:-8881690 | None:intergenic |
| GACGCCGAATCTTTGTTGTG+AGG | 0.620436 | 4.4:+8885442 | MS.gene28413:CDS |
| ATGCGTAGGATAAGCAGAGA+AGG | 0.621133 | 4.4:-8880048 | None:intergenic |
| GTGCATCTTTCGTCCAAGGA+CGG | 0.624419 | 4.4:-8885748 | None:intergenic |
| TTATGGTCATGATGAAGCTG+GGG | 0.624757 | 4.4:+8881086 | MS.gene28413:CDS |
| TTCAGAGAAGCTCTCCTGTG+GGG | 0.632335 | 4.4:+8883500 | MS.gene28413:CDS |
| TGTGAAAAGCTTTAAGAACA+CGG | 0.632469 | 4.4:-8880307 | None:intergenic |
| CAAACAACATCCTCATGAAG+CGG | 0.632543 | 4.4:-8885521 | None:intergenic |
| TTGGACGAAAGATGCACCTG+TGG | 0.633254 | 4.4:+8885754 | MS.gene28413:CDS |
| GGTTTGGTGTATGACCCCAC+AGG | 0.633665 | 4.4:-8883514 | None:intergenic |
| AAACCTGAACTGTATCACAT+CGG | 0.634425 | 4.4:-8883221 | None:intergenic |
| CTCTGATGCATATCCAGGAG+TGG | 0.634582 | 4.4:-8883548 | None:intergenic |
| GGATGGTGTTAAGAGGAGTG+AGG | 0.635793 | 4.4:+8885175 | MS.gene28413:CDS |
| GCGGGAGAGGAAGTAGGTGT+GGG | 0.639111 | 4.4:+8885353 | MS.gene28413:CDS |
| GGAAGGCATTGAACCTCCCA+TGG | 0.640149 | 4.4:+8882749 | MS.gene28413:CDS |
| AAGGCTCTCATGATATCACC+GGG | 0.640580 | 4.4:-8883001 | None:intergenic |
| GCGAGTACCTCTGGAACGAA+GGG | 0.641161 | 4.4:-8880140 | None:intergenic |
| AAGACATTATCACCACCCAT+AGG | 0.647434 | 4.4:-8885644 | None:intergenic |
| ATGAACAAGAGCATCAACCA+GGG | 0.649477 | 4.4:-8886116 | None:intergenic |
| TGCACCTGTGGCTTATGAGA+GGG | 0.650778 | 4.4:+8885766 | MS.gene28413:CDS |
| CAGAACTTCAGCAATCAGGA+AGG | 0.652209 | 4.4:-8883157 | None:intergenic |
| TCCACCATGACCGAACACTC+AGG | 0.653275 | 4.4:-8882705 | None:intergenic |
| GGAGGTTCAAGTGGTAAAGG+AGG | 0.657296 | 4.4:+8885415 | MS.gene28413:CDS |
| GCACTCATACAGAGGTCATG+TGG | 0.658940 | 4.4:+8882097 | MS.gene28413:CDS |
| GCACCTGTGGCTTATGAGAG+GGG | 0.660521 | 4.4:+8885767 | MS.gene28413:CDS |
| ACTTAGAACACTCCTCCTTG+CGG | 0.661748 | 4.4:-8886219 | None:intergenic |
| TTATCGCATACCACCATGGG+AGG | 0.664402 | 4.4:-8882762 | None:intergenic |
| GGAACGATTGCTCCATGTGG+CGG | 0.666888 | 4.4:+8882893 | MS.gene28413:CDS |
| GGCACTCATTGGACTCAATG+GGG | 0.670357 | 4.4:+8883886 | MS.gene28413:CDS |
| AGCTTCGGCTGACAAACGAA+GGG | 0.671384 | 4.4:-8884556 | None:intergenic |
| AGGTGTGGGTAAGAAAGTGG+TGG | 0.677668 | 4.4:+8885367 | MS.gene28413:CDS |
| ATTGGGAAAAGGGACTCTGG+TGG | 0.681378 | 4.4:+8881886 | MS.gene28413:CDS |
| AAAAGCTTTAAGAACACGGG+TGG | 0.682092 | 4.4:-8880303 | None:intergenic |
| TGTCAGCCGAAGCTTTACGG+TGG | 0.682406 | 4.4:+8884565 | MS.gene28413:CDS |
| GATAGGAACAGAGAAGCGAG+AGG | 0.690097 | 4.4:-8880114 | None:intergenic |
| GGTGGAACGATTGCTCCATG+TGG | 0.694007 | 4.4:+8882890 | MS.gene28413:CDS |
| GTTGAAGGTAAGGATGTGGA+TGG | 0.700502 | 4.4:+8885158 | MS.gene28413:CDS |
| CCTGAACTGTATCACATCGG+GGG | 0.701546 | 4.4:-8883218 | None:intergenic |
| ACCTGAACTGTATCACATCG+GGG | 0.703191 | 4.4:-8883219 | None:intergenic |
| TATGGTCATGATGAAGCTGG+GGG | 0.710054 | 4.4:+8881087 | MS.gene28413:CDS |
| AGGCATTGAACCTCCCATGG+TGG | 0.710958 | 4.4:+8882752 | MS.gene28413:CDS |
| CATGGTGGATAACTGCTATG+GGG | 0.720511 | 4.4:+8882719 | MS.gene28413:CDS |
| ACATTCAATTAACCGCAAGG+AGG | 0.741187 | 4.4:+8886207 | MS.gene28413:CDS |
| TGAACAAGAGCATCAACCAG+GGG | 0.746177 | 4.4:-8886115 | None:intergenic |
| AGTTTGATAAAGAATCCTGG+TGG | 0.766897 | 4.4:+8882872 | MS.gene28413:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 8880029 | 8890713 | 8880029 | ID=MS.gene28413 |
| chr4.4 | mRNA | 8880029 | 8890713 | 8880029 | ID=MS.gene28413.t1;Parent=MS.gene28413 |
| chr4.4 | exon | 8880029 | 8880154 | 8880029 | ID=MS.gene28413.t1.exon1;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8880029 | 8880154 | 8880029 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8880236 | 8880349 | 8880236 | ID=MS.gene28413.t1.exon2;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8880236 | 8880349 | 8880236 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8881066 | 8881176 | 8881066 | ID=MS.gene28413.t1.exon3;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8881066 | 8881176 | 8881066 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8881650 | 8881712 | 8881650 | ID=MS.gene28413.t1.exon4;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8881650 | 8881712 | 8881650 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8881841 | 8881954 | 8881841 | ID=MS.gene28413.t1.exon5;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8881841 | 8881954 | 8881841 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8882031 | 8882180 | 8882031 | ID=MS.gene28413.t1.exon6;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8882031 | 8882180 | 8882031 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8882696 | 8882773 | 8882696 | ID=MS.gene28413.t1.exon7;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8882696 | 8882773 | 8882696 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8882851 | 8883072 | 8882851 | ID=MS.gene28413.t1.exon8;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8882851 | 8883072 | 8882851 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8883153 | 8883239 | 8883153 | ID=MS.gene28413.t1.exon9;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8883153 | 8883239 | 8883153 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8883451 | 8883570 | 8883451 | ID=MS.gene28413.t1.exon10;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8883451 | 8883570 | 8883451 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8883702 | 8883798 | 8883702 | ID=MS.gene28413.t1.exon11;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8883702 | 8883798 | 8883702 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8883883 | 8883924 | 8883883 | ID=MS.gene28413.t1.exon12;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8883883 | 8883924 | 8883883 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8884341 | 8884387 | 8884341 | ID=MS.gene28413.t1.exon13;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8884341 | 8884387 | 8884341 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8884533 | 8884621 | 8884533 | ID=MS.gene28413.t1.exon14;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8884533 | 8884621 | 8884533 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8885103 | 8886505 | 8885103 | ID=MS.gene28413.t1.exon15;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8885103 | 8886505 | 8885103 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| chr4.4 | exon | 8890628 | 8890713 | 8890628 | ID=MS.gene28413.t1.exon16;Parent=MS.gene28413.t1 |
| chr4.4 | CDS | 8890628 | 8890713 | 8890628 | ID=cds.MS.gene28413.t1;Parent=MS.gene28413.t1 |
| Gene Sequence |
| Protein sequence |
