Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28442.t1 | RHN43062.1 | 68.9 | 299 | 49 | 6 | 1 | 295 | 1 | 259 | 2.50E-30 | 142.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28442.t1 | A0A396GPN1 | 68.9 | 299 | 49 | 6 | 1 | 295 | 1 | 259 | 1.8e-30 | 142.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene28442 | 0.826778 | 2.25E-54 | -1.69E-46 |
| MS.gene049711 | MS.gene28442 | 0.803565 | 3.28E-49 | -1.69E-46 |
| MS.gene050238 | MS.gene28442 | 0.815735 | 7.94E-52 | -1.69E-46 |
| MS.gene051279 | MS.gene28442 | 0.811068 | 8.42E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene28442 | 0.825584 | 4.33E-54 | -1.69E-46 |
| MS.gene054178 | MS.gene28442 | 0.820781 | 5.72E-53 | -1.69E-46 |
| MS.gene058649 | MS.gene28442 | 0.803683 | 3.10E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene28442 | 0.806878 | 6.64E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28442.t1 | MTR_8g092670 | 71.951 | 164 | 16 | 6 | 132 | 295 | 17 | 150 | 4.90e-55 | 176 |
| MS.gene28442.t1 | MTR_8g092675 | 92.308 | 78 | 2 | 1 | 1 | 74 | 1 | 78 | 2.89e-43 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAGGTGCAATATCATTT+TGG | 0.207097 | 4.4:+8621218 | None:intergenic |
| ACAAGAATCTACAGTTATTA+AGG | 0.256810 | 4.4:-8621395 | MS.gene28442:CDS |
| TCTTTAACCTGTTCAGGTTC+AGG | 0.278032 | 4.4:+8628976 | None:intergenic |
| GTGTCTTCTTTAACCTGTTC+AGG | 0.281388 | 4.4:+8628970 | None:intergenic |
| GTAGATTCTTGTTCTGTAGA+TGG | 0.312343 | 4.4:+8621406 | None:intergenic |
| GTCTTCACCACAGTTACTTC+AGG | 0.341416 | 4.4:+8621202 | None:intergenic |
| GGAGATTCATGTTTCAAAGT+TGG | 0.408662 | 4.4:+8621472 | None:intergenic |
| TTCACAACAGTTTCTTCAGC+TGG | 0.423038 | 4.4:+8621247 | None:intergenic |
| TTCTGTCTTCACAATCTCTT+CGG | 0.442450 | 4.4:+8621321 | None:intergenic |
| TTCTCTTCTATGCTAGATGT+TGG | 0.446295 | 4.4:+8620995 | None:intergenic |
| ATTAGAAACAGAGAAACCTT+TGG | 0.449166 | 4.4:-8621515 | MS.gene28442:CDS |
| AAACCAAAGGTTACGAAGGA+TGG | 0.459100 | 4.4:-8629018 | MS.gene28442:CDS |
| GAAGGCACCTGAACCTGAAC+AGG | 0.460385 | 4.4:-8628983 | MS.gene28442:CDS |
| ACTGTCAGCATCAGAAGCCT+TGG | 0.483191 | 4.4:+8620940 | None:intergenic |
| ATCACTTTCTCCGCAGGTGG+AGG | 0.497231 | 4.4:+8621067 | None:intergenic |
| ATCTGTGGAGAACAAAATAG+TGG | 0.502395 | 4.4:-8621020 | MS.gene28442:CDS |
| AAGCTTGTTGTTCAACAAAG+AGG | 0.514250 | 4.4:-8628836 | MS.gene28442:intron |
| AGTTATTAAGGTTGATGTTG+AGG | 0.515319 | 4.4:-8621383 | MS.gene28442:CDS |
| AACCAAAGGTTACGAAGGAT+GGG | 0.516776 | 4.4:-8629017 | MS.gene28442:CDS |
| TCAATCACTTTCTCCGCAGG+TGG | 0.530836 | 4.4:+8621064 | None:intergenic |
| ACTTCAATCACTTTCTCCGC+AGG | 0.536439 | 4.4:+8621061 | None:intergenic |
| TTTAACAAATGCAGAATCTG+TGG | 0.554816 | 4.4:-8621035 | MS.gene28442:CDS |
| AGAAGGTGAGAAAGCTTCAG+AGG | 0.560763 | 4.4:-8621170 | MS.gene28442:CDS |
| AGAAACAGAGAAACCTTTGG+AGG | 0.576894 | 4.4:-8621512 | MS.gene28442:CDS |
| GACTATTCTCATTGAAAATG+TGG | 0.591672 | 4.4:-8621095 | MS.gene28442:CDS |
| ACCAAAGGTTACGAAGGATG+GGG | 0.594184 | 4.4:-8629016 | MS.gene28442:CDS |
| GAATGAAGATACAATTACAA+AGG | 0.619093 | 4.4:-8621590 | MS.gene28442:CDS |
| CACTGTTGAAACCAAAGTGG+AGG | 0.620060 | 4.4:-8628950 | MS.gene28442:CDS |
| TATTCTCATTGAAAATGTGG+TGG | 0.622958 | 4.4:-8621092 | MS.gene28442:CDS |
| TGCGGAGAAAGTGATTGAAG+TGG | 0.637663 | 4.4:-8621059 | MS.gene28442:CDS |
| AACTAAACCAAAGGTTACGA+AGG | 0.648382 | 4.4:-8629022 | MS.gene28442:CDS |
| GGGTTGTGCAACTAAACCAA+AGG | 0.654419 | 4.4:-8629031 | MS.gene28442:CDS |
| TGGGGTAGAGCATATATCCA+AGG | 0.654752 | 4.4:-8620957 | MS.gene28442:CDS |
| TGTGGTGGTTCCTCCACCTG+CGG | 0.658290 | 4.4:-8621077 | MS.gene28442:CDS |
| AGACACTGTTGAAACCAAAG+TGG | 0.670371 | 4.4:-8628953 | MS.gene28442:CDS |
| CAAGAGCAATTGTTGCAGAG+TGG | 0.680159 | 4.4:-8628919 | MS.gene28442:CDS |
| GTGAAGACAGAAACACAAGA+AGG | 0.686055 | 4.4:-8621187 | MS.gene28442:CDS |
| CCAAAGGTTACGAAGGATGG+GGG | 0.695094 | 4.4:-8629015 | MS.gene28442:CDS |
| TATTGCACCTGAAGTAACTG+TGG | 0.731781 | 4.4:-8621209 | MS.gene28442:CDS |
| CTGCGATTGAAAGAAATGGG+GGG | 0.733842 | 4.4:-8629051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 8620919 | 8629058 | 8620919 | ID=MS.gene28442 |
| chr4.4 | mRNA | 8620919 | 8629058 | 8620919 | ID=MS.gene28442.t1;Parent=MS.gene28442 |
| chr4.4 | exon | 8628837 | 8629058 | 8628837 | ID=MS.gene28442.t1.exon1;Parent=MS.gene28442.t1 |
| chr4.4 | CDS | 8628837 | 8629058 | 8628837 | ID=cds.MS.gene28442.t1;Parent=MS.gene28442.t1 |
| chr4.4 | exon | 8620919 | 8621611 | 8620919 | ID=MS.gene28442.t1.exon2;Parent=MS.gene28442.t1 |
| chr4.4 | CDS | 8620919 | 8621611 | 8620919 | ID=cds.MS.gene28442.t1;Parent=MS.gene28442.t1 |
| Gene Sequence |
| Protein sequence |
