Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28613.t1 | PNY03890.1 | 62.9 | 167 | 11 | 3 | 1 | 116 | 1 | 167 | 2.70E-46 | 194.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28613.t1 | A0A2K3NLI1 | 62.9 | 167 | 11 | 3 | 1 | 116 | 1 | 167 | 2.0e-46 | 194.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene28613 | 0.819216 | 1.30E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene28613 | 0.815511 | 8.90E-52 | -1.69E-46 |
| MS.gene050116 | MS.gene28613 | 0.808893 | 2.48E-50 | -1.69E-46 |
| MS.gene050359 | MS.gene28613 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene28613 | 0.852724 | 3.83E-61 | -1.69E-46 |
| MS.gene052776 | MS.gene28613 | 0.818041 | 2.41E-52 | -1.69E-46 |
| MS.gene053143 | MS.gene28613 | 0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene058638 | MS.gene28613 | 0.819369 | 1.20E-52 | -1.69E-46 |
| MS.gene058639 | MS.gene28613 | 0.811522 | 6.71E-51 | -1.69E-46 |
| MS.gene059007 | MS.gene28613 | 0.824131 | 9.53E-54 | -1.69E-46 |
| MS.gene061006 | MS.gene28613 | 0.80061 | 1.33E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28613.t1 | MTR_7g111080 | 64.458 | 166 | 8 | 3 | 1 | 115 | 1 | 166 | 2.13e-59 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28613.t1 | AT5G24710 | 54.491 | 167 | 23 | 4 | 1 | 114 | 1 | 167 | 6.02e-47 | 161 |
Find 26 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAGTTTCTGTTTGGAAC+TGG | 0.345512 | 7.3:-4607909 | MS.gene28613:CDS |
| ATTCAGATCGAGTTTCTGTT+TGG | 0.396019 | 7.3:-4607915 | MS.gene28613:CDS |
| TTGTTATCAAGCTCTTGCTT+TGG | 0.427944 | 7.3:+4606369 | None:intergenic |
| ATGGATGCGTCGGATGCAAT+TGG | 0.437066 | 7.3:+4607956 | None:intergenic |
| TCTCCTGCCCCATCAACAAA+AGG | 0.473766 | 7.3:-4606468 | MS.gene28613:CDS |
| CCTCCACGAATGGCTTCTGT+CGG | 0.473800 | 7.3:+4606689 | None:intergenic |
| TTGGAACTGGGAACACCGTC+AGG | 0.484214 | 7.3:-4607896 | MS.gene28613:intron |
| TCGAGTTTCTGTTTGGAACT+GGG | 0.490366 | 7.3:-4607908 | MS.gene28613:CDS |
| TAATCGTTCTGCAGCGGCAG+AGG | 0.499251 | 7.3:-4606523 | MS.gene28613:intron |
| TAATTAAAACTTACCAGTGT+AGG | 0.500832 | 7.3:+4606343 | None:intergenic |
| AATTGCATCCGACGCATCCA+TGG | 0.521168 | 7.3:-4607954 | MS.gene28613:CDS |
| ATCCTACCTTCCTCCACGAA+TGG | 0.526369 | 7.3:+4606679 | None:intergenic |
| TCATCAGCAGTAACCATCCA+TGG | 0.551384 | 7.3:+4607937 | None:intergenic |
| CTCCTGCCCCATCAACAAAA+GGG | 0.552691 | 7.3:-4606467 | MS.gene28613:CDS |
| CAGAAGCCATTCGTGGAGGA+AGG | 0.564143 | 7.3:-4606685 | MS.gene28613:intron |
| AATTAAAACTTACCAGTGTA+GGG | 0.565846 | 7.3:+4606344 | None:intergenic |
| GCATCCGACGCATCCATGGA+TGG | 0.578685 | 7.3:-4607950 | MS.gene28613:CDS |
| CACGGCCACGCATCGTCACC+AGG | 0.582340 | 7.3:+4606397 | None:intergenic |
| AAACCGACAGAAGCCATTCG+TGG | 0.586570 | 7.3:-4606692 | MS.gene28613:CDS |
| GCTCTTGCTTTGGTACATCA+CGG | 0.624623 | 7.3:+4606379 | None:intergenic |
| GTAACCATCCATGGATGCGT+CGG | 0.624825 | 7.3:+4607946 | None:intergenic |
| TTGGACCTGGTGACGATGCG+TGG | 0.625252 | 7.3:-4606402 | MS.gene28613:CDS |
| GCTGAAGTATGTACAGCTGT+TGG | 0.649234 | 7.3:+4606498 | None:intergenic |
| TTGATAACAAGTCCCTACAC+TGG | 0.657074 | 7.3:-4606356 | MS.gene28613:CDS |
| CTGCCCCATCAACAAAAGGG+AGG | 0.684707 | 7.3:-4606464 | MS.gene28613:CDS |
| CCGACAGAAGCCATTCGTGG+AGG | 0.698024 | 7.3:-4606689 | MS.gene28613:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCAAAATAAAATAAAATAAA+TGG | + | chr7.3:4607633-4607652 | None:intergenic | 10.0% |
| !!! | CCATTTATTTTATTTTATTT+TGG | - | chr7.3:4607630-4607649 | MS.gene28613:intron | 10.0% |
| !!! | AAAATTGATTTTGACATATT+TGG | - | chr7.3:4607031-4607050 | MS.gene28613:intron | 15.0% |
| !!! | TTTAAACAAAGCTATATTTT+TGG | - | chr7.3:4607938-4607957 | MS.gene28613:CDS | 15.0% |
| !! | AACTTATGAAAACAACTTAT+AGG | - | chr7.3:4606776-4606795 | MS.gene28613:intron | 20.0% |
| !!! | AATTTGTAGCTTTTAATTCT+AGG | - | chr7.3:4607108-4607127 | MS.gene28613:intron | 20.0% |
| !!! | ACTTTTTTAAGCATATTTTG+CGG | - | chr7.3:4607564-4607583 | MS.gene28613:intron | 20.0% |
| !!! | ATACGATAATTTGTAATCAT+TGG | - | chr7.3:4607438-4607457 | MS.gene28613:intron | 20.0% |
| !!! | GTTTTTCTTTATGCATAAAA+CGG | + | chr7.3:4607541-4607560 | None:intergenic | 20.0% |
| !!! | TACGATAATTTGTAATCATT+GGG | - | chr7.3:4607439-4607458 | MS.gene28613:intron | 20.0% |
| !!! | TGATTTGAGATTTTATCATT+GGG | + | chr7.3:4607391-4607410 | None:intergenic | 20.0% |
| ! | ATTTGTAATTGTTACACACT+TGG | - | chr7.3:4606687-4606706 | MS.gene28613:CDS | 25.0% |
| ! | CAATGATAAAATCTCAAATC+AGG | - | chr7.3:4607390-4607409 | MS.gene28613:intron | 25.0% |
| ! | GGATATAAAAACACAAAACA+AGG | + | chr7.3:4607708-4607727 | None:intergenic | 25.0% |
| !! | TTAAGTTGTTTATCCAAACA+TGG | - | chr7.3:4606902-4606921 | MS.gene28613:intron | 25.0% |
| !!! | CTGATTTGAGATTTTATCAT+TGG | + | chr7.3:4607392-4607411 | None:intergenic | 25.0% |
| !!! | GATTGAAGCTTTTTTTTTTC+AGG | - | chr7.3:4606539-4606558 | MS.gene28613:intron | 25.0% |
| !!! | TACTAGTTTTCGTGTATATT+TGG | - | chr7.3:4606995-4607014 | MS.gene28613:intron | 25.0% |
| CAGTATTTGTGACATTGTTT+CGG | - | chr7.3:4606751-4606770 | MS.gene28613:intron | 30.0% | |
| GGTTATGCTCTTACATTATT+AGG | - | chr7.3:4607481-4607500 | MS.gene28613:intron | 30.0% | |
| TAAGAATGCCAAAAATGAAC+CGG | + | chr7.3:4607287-4607306 | None:intergenic | 30.0% | |
| ! | AAAATCAATTTTTCCCCTAG+TGG | + | chr7.3:4607023-4607042 | None:intergenic | 30.0% |
| ! | AAGGTAAAGTGATTTGTGTT+TGG | + | chr7.3:4607186-4607205 | None:intergenic | 30.0% |
| ! | ATATCCTTTTCTGATTTCCA+AGG | - | chr7.3:4607722-4607741 | MS.gene28613:intron | 30.0% |
| !! | AATCAGGTTAACTTGCTTTA+TGG | - | chr7.3:4607406-4607425 | MS.gene28613:intron | 30.0% |
| !! | ACTTGCTTCATTTTTCGTTT+TGG | - | chr7.3:4606489-4606508 | MS.gene28613:CDS | 30.0% |
| !! | ATCAGGTTAACTTGCTTTAT+GGG | - | chr7.3:4607407-4607426 | MS.gene28613:intron | 30.0% |
| !!! | TTACCGAGTCATTGTTTTTT+GGG | - | chr7.3:4607323-4607342 | MS.gene28613:intron | 30.0% |
| AATGAAGCAAGTTTACCTGA+CGG | + | chr7.3:4606481-4606500 | None:intergenic | 35.0% | |
| ATCCCCAAAAAACAATGACT+CGG | + | chr7.3:4607329-4607348 | None:intergenic | 35.0% | |
| ATTCAGATCGAGTTTCTGTT+TGG | - | chr7.3:4606444-4606463 | MS.gene28613:CDS | 35.0% | |
| CGATAAAAGCAAATGTGCAA+GGG | + | chr7.3:4606928-4606947 | None:intergenic | 35.0% | |
| GGGATTTGACTAGTAATCAA+TGG | - | chr7.3:4607344-4607363 | MS.gene28613:intron | 35.0% | |
| TAGTATCGCAGTAATGTTCA+TGG | + | chr7.3:4606980-4606999 | None:intergenic | 35.0% | |
| TGGTTACACATTTCTGATAC+TGG | + | chr7.3:4607613-4607632 | None:intergenic | 35.0% | |
| TTGTTATCAAGCTCTTGCTT+TGG | + | chr7.3:4607993-4608012 | None:intergenic | 35.0% | |
| ! | ATGTAAGAGCATAACCAAAC+AGG | + | chr7.3:4607477-4607496 | None:intergenic | 35.0% |
| ! | ATTTATGAGTTGAAAGCTGG+TGG | - | chr7.3:4606564-4606583 | MS.gene28613:intron | 35.0% |
| ! | GATCTTAACGATTTTATCGC+TGG | + | chr7.3:4606384-4606403 | None:intergenic | 35.0% |
| ! | GTGATTTATGAGTTGAAAGC+TGG | - | chr7.3:4606561-4606580 | MS.gene28613:intron | 35.0% |
| ! | GTGTATATTTGGTTCCACTA+GGG | - | chr7.3:4607006-4607025 | MS.gene28613:intron | 35.0% |
| ! | TCTGACTAAAAGTGAGTTGA+AGG | + | chr7.3:4607205-4607224 | None:intergenic | 35.0% |
| ! | TGTATATTTGGTTCCACTAG+GGG | - | chr7.3:4607007-4607026 | MS.gene28613:intron | 35.0% |
| ! | TTAACGATTTTATCGCTGGA+AGG | + | chr7.3:4606380-4606399 | None:intergenic | 35.0% |
| !! | ATGATGATGATGTACGCTTT+TGG | - | chr7.3:4607802-4607821 | MS.gene28613:intron | 35.0% |
| !! | CCTTGCACATTTGCTTTTAT+CGG | - | chr7.3:4606926-4606945 | MS.gene28613:intron | 35.0% |
| !! | TTGCTTTTATCGGATTCGTT+TGG | - | chr7.3:4606936-4606955 | MS.gene28613:intron | 35.0% |
| !!! | CAAAGCTATATTTTTGGACC+TGG | - | chr7.3:4607944-4607963 | MS.gene28613:CDS | 35.0% |
| !!! | GTTACCGAGTCATTGTTTTT+TGG | - | chr7.3:4607322-4607341 | MS.gene28613:intron | 35.0% |
| !!! | TACCGAGTCATTGTTTTTTG+GGG | - | chr7.3:4607324-4607343 | MS.gene28613:intron | 35.0% |
| !!! | TTTATTTTGGTAGAGTCCAG+AGG | - | chr7.3:4607643-4607662 | MS.gene28613:intron | 35.0% |
| AACATATCCTCCACAGTAAC+CGG | - | chr7.3:4607265-4607284 | MS.gene28613:intron | 40.0% | |
| CAAAAATGAACCGGTTACTG+TGG | + | chr7.3:4607278-4607297 | None:intergenic | 40.0% | |
| CCGATAAAAGCAAATGTGCA+AGG | + | chr7.3:4606929-4606948 | None:intergenic | 40.0% | |
| CGAACCTTGGAAATCAGAAA+AGG | + | chr7.3:4607729-4607748 | None:intergenic | 40.0% | |
| GCTTCAAGCAAAATCAACTC+TGG | + | chr7.3:4607085-4607104 | None:intergenic | 40.0% | |
| GGCATAGTATTGTTCCTGTT+TGG | - | chr7.3:4607460-4607479 | MS.gene28613:intron | 40.0% | |
| GTTACTGTGGAGGATATGTT+TGG | + | chr7.3:4607265-4607284 | None:intergenic | 40.0% | |
| TTACACACTTGGCGATTGTT+TGG | - | chr7.3:4606698-4606717 | MS.gene28613:CDS | 40.0% | |
| ! | AAATGCCTCCCTTTTGTTGA+TGG | + | chr7.3:4607903-4607922 | None:intergenic | 40.0% |
| ! | AATGCCTCCCTTTTGTTGAT+GGG | + | chr7.3:4607902-4607921 | None:intergenic | 40.0% |
| ! | ATCGAGTTTCTGTTTGGAAC+TGG | - | chr7.3:4606450-4606469 | MS.gene28613:CDS | 40.0% |
| ! | ATGTACGCTTTTGGCAACTT+TGG | - | chr7.3:4607811-4607830 | MS.gene28613:intron | 40.0% |
| ! | CGTGTATATTTGGTTCCACT+AGG | - | chr7.3:4607005-4607024 | MS.gene28613:intron | 40.0% |
| ! | TCGAGTTTCTGTTTGGAACT+GGG | - | chr7.3:4606451-4606470 | MS.gene28613:CDS | 40.0% |
| ! | TTGATAACAAGTCCCTACAC+TGG | - | chr7.3:4608003-4608022 | MS.gene28613:CDS | 40.0% |
| !! | AAAATTGGAGAAGCTTGCTG+AGG | - | chr7.3:4606614-4606633 | MS.gene28613:intron | 40.0% |
| !! | AAATTGGAGAAGCTTGCTGA+GGG | - | chr7.3:4606615-4606634 | MS.gene28613:intron | 40.0% |
| !! | CACAGTAACCGGTTCATTTT+TGG | - | chr7.3:4607276-4607295 | MS.gene28613:intron | 40.0% |
| !!! | ATGTTTGGTTTTGCAGTGAC+AGG | + | chr7.3:4607250-4607269 | None:intergenic | 40.0% |
| AAATGAACCGGTTACTGTGG+AGG | + | chr7.3:4607275-4607294 | None:intergenic | 45.0% | |
| AAATGTGCAAGGGCCATGTT+TGG | + | chr7.3:4606918-4606937 | None:intergenic | 45.0% | |
| CATGATAAGCAAGCGAACCT+TGG | + | chr7.3:4607742-4607761 | None:intergenic | 45.0% | |
| GCTCTTGCTTTGGTACATCA+CGG | + | chr7.3:4607983-4608002 | None:intergenic | 45.0% | |
| GCTGAAGTATGTACAGCTGT+TGG | + | chr7.3:4607864-4607883 | None:intergenic | 45.0% | |
| GTCACCTGCTTAACACTTCA+AGG | + | chr7.3:4607777-4607796 | None:intergenic | 45.0% | |
| TCATCAGCAGTAACCATCCA+TGG | + | chr7.3:4606425-4606444 | None:intergenic | 45.0% | |
| TTGGCATAATCGTTCTGCAG+CGG | - | chr7.3:4607830-4607849 | MS.gene28613:intron | 45.0% | |
| ! | ATGCCTCCCTTTTGTTGATG+GGG | + | chr7.3:4607901-4607920 | None:intergenic | 45.0% |
| ! | GTTGATGAACGACGGTTAGT+CGG | - | chr7.3:4606588-4606607 | MS.gene28613:intron | 45.0% |
| AAACCGACAGAAGCCATTCG+TGG | - | chr7.3:4607667-4607686 | MS.gene28613:intron | 50.0% | |
| AATTGCATCCGACGCATCCA+TGG | - | chr7.3:4606405-4606424 | MS.gene28613:CDS | 50.0% | |
| ATCCTACCTTCCTCCACGAA+TGG | + | chr7.3:4607683-4607702 | None:intergenic | 50.0% | |
| ATGGATGCGTCGGATGCAAT+TGG | + | chr7.3:4606406-4606425 | None:intergenic | 50.0% | |
| CTCCTGCCCCATCAACAAAA+GGG | - | chr7.3:4607892-4607911 | MS.gene28613:intron | 50.0% | |
| GTAACCATCCATGGATGCGT+CGG | + | chr7.3:4606416-4606435 | None:intergenic | 50.0% | |
| TCTCCTGCCCCATCAACAAA+AGG | - | chr7.3:4607891-4607910 | MS.gene28613:intron | 50.0% | |
| ! | ACGGTTAGTCGGCGCAAAAT+TGG | - | chr7.3:4606599-4606618 | MS.gene28613:intron | 50.0% |
| ! | CTCGCCTTGAAGTGTTAAGC+AGG | - | chr7.3:4607770-4607789 | MS.gene28613:intron | 50.0% |
| !! | AAGCTTGCTGAGGGAGAAAG+AGG | - | chr7.3:4606624-4606643 | MS.gene28613:intron | 50.0% |
| !! | CTGGTGGAGTTGATGAACGA+CGG | - | chr7.3:4606580-4606599 | MS.gene28613:intron | 50.0% |
| AGCCATTCGTGGAGGAAGGT+AGG | - | chr7.3:4607678-4607697 | MS.gene28613:intron | 55.0% | |
| CAGAAGCCATTCGTGGAGGA+AGG | - | chr7.3:4607674-4607693 | MS.gene28613:intron | 55.0% | |
| CCTCCACGAATGGCTTCTGT+CGG | + | chr7.3:4607673-4607692 | None:intergenic | 55.0% | |
| CTGCCCCATCAACAAAAGGG+AGG | - | chr7.3:4607895-4607914 | MS.gene28613:intron | 55.0% | |
| TAATCGTTCTGCAGCGGCAG+AGG | - | chr7.3:4607836-4607855 | MS.gene28613:intron | 55.0% | |
| TTGGAACTGGGAACACCGTC+AGG | - | chr7.3:4606463-4606482 | MS.gene28613:CDS | 55.0% | |
| !! | CTCCCTTTTGTTGATGGGGC+AGG | + | chr7.3:4607897-4607916 | None:intergenic | 55.0% |
| !!! | GGCTTCTGTCGGTTTTCCTC+TGG | + | chr7.3:4607662-4607681 | None:intergenic | 55.0% |
| CCGACAGAAGCCATTCGTGG+AGG | - | chr7.3:4607670-4607689 | MS.gene28613:intron | 60.0% | |
| GCATCCGACGCATCCATGGA+TGG | - | chr7.3:4606409-4606428 | MS.gene28613:CDS | 60.0% | |
| ! | TTGGACCTGGTGACGATGCG+TGG | - | chr7.3:4607957-4607976 | MS.gene28613:CDS | 60.0% |
| CACGGCCACGCATCGTCACC+AGG | + | chr7.3:4607965-4607984 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 4606353 | 4608028 | 4606353 | ID=MS.gene28613 |
| chr7.3 | mRNA | 4606353 | 4608028 | 4606353 | ID=MS.gene28613.t1;Parent=MS.gene28613 |
| chr7.3 | exon | 4607897 | 4608028 | 4607897 | ID=MS.gene28613.t1.exon1;Parent=MS.gene28613.t1 |
| chr7.3 | CDS | 4607897 | 4608028 | 4607897 | ID=cds.MS.gene28613.t1;Parent=MS.gene28613.t1 |
| chr7.3 | exon | 4606686 | 4606725 | 4606686 | ID=MS.gene28613.t1.exon2;Parent=MS.gene28613.t1 |
| chr7.3 | CDS | 4606686 | 4606725 | 4606686 | ID=cds.MS.gene28613.t1;Parent=MS.gene28613.t1 |
| chr7.3 | exon | 4606353 | 4606531 | 4606353 | ID=MS.gene28613.t1.exon3;Parent=MS.gene28613.t1 |
| chr7.3 | CDS | 4606353 | 4606531 | 4606353 | ID=cds.MS.gene28613.t1;Parent=MS.gene28613.t1 |
| Gene Sequence |
| Protein sequence |
