Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28654.t1 | XP_003625923.1 | 95.4 | 303 | 11 | 2 | 1 | 300 | 1 | 303 | 2.30E-161 | 578.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28654.t1 | Q8LEA8 | 47.1 | 333 | 141 | 5 | 1 | 300 | 5 | 335 | 3.6e-83 | 309.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28654.t1 | G7KRX0 | 95.4 | 303 | 11 | 2 | 1 | 300 | 1 | 303 | 1.6e-161 | 578.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052641 | MS.gene28654 | 0.842901 | 1.95E-58 | -1.69E-46 |
| MS.gene055505 | MS.gene28654 | 0.858333 | 8.85E-63 | -1.69E-46 |
| MS.gene057199 | MS.gene28654 | 0.825062 | 5.75E-54 | -1.69E-46 |
| MS.gene057668 | MS.gene28654 | 0.858787 | 6.48E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28654.t1 | MTR_7g108820 | 98.026 | 304 | 3 | 2 | 1 | 301 | 1 | 304 | 0.0 | 615 |
| MS.gene28654.t1 | MTR_8g058520 | 98.026 | 304 | 3 | 2 | 1 | 301 | 1 | 304 | 0.0 | 615 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28654.t1 | AT4G02440 | 46.707 | 334 | 143 | 5 | 1 | 301 | 5 | 336 | 1.42e-105 | 311 |
Find 54 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGATAATGGTCATGTTTC+TGG | 0.130245 | 7.2:-4993765 | MS.gene28654:CDS |
| CAAATGAATGTTCTTCTTAT+TGG | 0.137353 | 7.2:-4994283 | MS.gene28654:CDS |
| AGGGCTTATGTTTGTGATAA+TGG | 0.254503 | 7.2:-4993777 | MS.gene28654:CDS |
| CACATTTAGCTACAGGAAAA+TGG | 0.257698 | 7.2:-4994100 | MS.gene28654:CDS |
| ACCTTTCTCTTCTTAGCTTC+TGG | 0.278200 | 7.2:+4994140 | None:intergenic |
| TGTTGGTGTTGTTGTTCATC+TGG | 0.306138 | 7.2:+4994356 | None:intergenic |
| GCTAAGAAGAGAAAGGTTAA+TGG | 0.338615 | 7.2:-4994134 | MS.gene28654:CDS |
| GTTTATGAAGAGAAAGATCT+TGG | 0.359162 | 7.2:+4994438 | None:intergenic |
| TTTGTTCTTGTAATCATACT+TGG | 0.383121 | 7.2:-4993866 | MS.gene28654:CDS |
| TAATCGGAAGAAGATTGATT+TGG | 0.384060 | 7.2:-4993898 | MS.gene28654:CDS |
| TGAAGCTCATCATCTTCAAC+AGG | 0.384826 | 7.2:-4994216 | MS.gene28654:CDS |
| ATGATGGTGAACCTGTTGTT+AGG | 0.390801 | 7.2:-4993797 | MS.gene28654:CDS |
| ATGGTCATGTTTCTGGTGCT+TGG | 0.396836 | 7.2:-4993758 | MS.gene28654:CDS |
| TTGAAGAAGACCCTCGTGAT+TGG | 0.397252 | 7.2:-4994568 | MS.gene28654:CDS |
| TTGGCCAGGTTGTGTTCATT+TGG | 0.410463 | 7.2:-4994006 | MS.gene28654:CDS |
| CTAAGAAGAGAAAGGTTAAT+GGG | 0.420309 | 7.2:-4994133 | MS.gene28654:CDS |
| TGTTCTTCCAACAGAAATCA+TGG | 0.422948 | 7.2:+4994504 | None:intergenic |
| CTCTCATCCATGATTTCTGT+TGG | 0.424893 | 7.2:-4994511 | MS.gene28654:CDS |
| ATTGTTTATACGTTTGTGAT+TGG | 0.426128 | 7.2:-4994025 | MS.gene28654:CDS |
| ATTTCAAGAAGACACGTGTT+TGG | 0.435090 | 7.2:-4993941 | MS.gene28654:CDS |
| TTGAGTTCACATTTAGCTAC+AGG | 0.438504 | 7.2:-4994107 | MS.gene28654:CDS |
| GAAGAAAGAAGAAGTTCAGA+AGG | 0.446221 | 7.2:+4994464 | None:intergenic |
| GTTGTTGATGATGATGTAAT+AGG | 0.451297 | 7.2:+4994332 | None:intergenic |
| TTGTTCTTGTAATCATACTT+GGG | 0.459848 | 7.2:-4993865 | MS.gene28654:CDS |
| AAACTACCCTTCTGTTGTCC+TGG | 0.461163 | 7.2:-4994419 | MS.gene28654:CDS |
| CTGTGAATGATATTAGTAAT+CGG | 0.469652 | 7.2:-4993914 | MS.gene28654:CDS |
| TGAAGAAGACCCTCGTGATT+GGG | 0.483756 | 7.2:-4994567 | MS.gene28654:CDS |
| GAAGAACATTCATTTGTTTG+AGG | 0.484296 | 7.2:+4994290 | None:intergenic |
| TTTCTCTTCTTAGCTTCTGG+TGG | 0.489673 | 7.2:+4994143 | None:intergenic |
| GAACATTCATTTGTTTGAGG+TGG | 0.490824 | 7.2:+4994293 | None:intergenic |
| GTTGGTGTTGTTGTTCATCT+GGG | 0.491428 | 7.2:+4994357 | None:intergenic |
| CAAACATAAGCCCTAACAAC+AGG | 0.496612 | 7.2:+4993786 | None:intergenic |
| ATGATGATGTAATAGGTTGT+TGG | 0.497159 | 7.2:+4994339 | None:intergenic |
| AACGAGGAAGAGATTAGAAC+TGG | 0.503126 | 7.2:-4994179 | MS.gene28654:CDS |
| AAGAATGATCAAACGTACAT+AGG | 0.511746 | 7.2:+4993726 | None:intergenic |
| TGTCCTGGTCCTCTTCATGC+TGG | 0.523403 | 7.2:-4994404 | MS.gene28654:CDS |
| TTTATGAAGAGAAAGATCTT+GGG | 0.527438 | 7.2:+4994439 | None:intergenic |
| ACCAGAAGCTAAGAAGAGAA+AGG | 0.540167 | 7.2:-4994141 | MS.gene28654:CDS |
| GGATTTGGTTATCATGATGA+TGG | 0.549376 | 7.2:-4993813 | MS.gene28654:CDS |
| AGTTCTAATCTCTTCCTCGT+TGG | 0.559801 | 7.2:+4994181 | None:intergenic |
| AAGAGGACCAGGACAACAGA+AGG | 0.566286 | 7.2:+4994412 | None:intergenic |
| TGATGGTGAACCTGTTGTTA+GGG | 0.575949 | 7.2:-4993796 | MS.gene28654:CDS |
| GAAAGACGAGCCCAATCACG+AGG | 0.597495 | 7.2:+4994557 | None:intergenic |
| ATACCAGCATGAAGAGGACC+AGG | 0.606219 | 7.2:+4994401 | None:intergenic |
| AAGAGAATACCAGCATGAAG+AGG | 0.613643 | 7.2:+4994395 | None:intergenic |
| TCTTCCAAATGAACACAACC+TGG | 0.613786 | 7.2:+4994002 | None:intergenic |
| TGGACTCTTAGTAGAGAACA+AGG | 0.622079 | 7.2:-4994080 | MS.gene28654:CDS |
| TTATACGTTTGTGATTGGCC+AGG | 0.624893 | 7.2:-4994020 | MS.gene28654:CDS |
| TGAGGTGGAAGAGATGATGA+AGG | 0.629257 | 7.2:+4994308 | None:intergenic |
| TTGTTCATCTGGGAAATCAG+CGG | 0.640290 | 7.2:+4994367 | None:intergenic |
| AGAGGACCAGGACAACAGAA+GGG | 0.650940 | 7.2:+4994413 | None:intergenic |
| GGGACAAGAACAGACCAACG+AGG | 0.713255 | 7.2:-4994195 | MS.gene28654:CDS |
| GAAGCTCATCATCTTCAACA+GGG | 0.732297 | 7.2:-4994215 | MS.gene28654:CDS |
| AAAGACGAGCCCAATCACGA+GGG | 0.734594 | 7.2:+4994558 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAATTCTGAAGATTTTA+TGG | - | chr7.2:4993756-4993775 | MS.gene28654:CDS | 15.0% |
| ! | ATTGTTTATACGTTTGTGAT+TGG | - | chr7.2:4994326-4994345 | MS.gene28654:CDS | 25.0% |
| ! | CAAATGAATGTTCTTCTTAT+TGG | - | chr7.2:4994068-4994087 | MS.gene28654:CDS | 25.0% |
| ! | CTGTGAATGATATTAGTAAT+CGG | - | chr7.2:4994437-4994456 | MS.gene28654:CDS | 25.0% |
| ! | TTGTTCTTGTAATCATACTT+GGG | - | chr7.2:4994486-4994505 | MS.gene28654:CDS | 25.0% |
| ! | TTTATGAAGAGAAAGATCTT+GGG | + | chr7.2:4993915-4993934 | None:intergenic | 25.0% |
| ! | TTTGTTCTTGTAATCATACT+TGG | - | chr7.2:4994485-4994504 | MS.gene28654:CDS | 25.0% |
| !!! | AATTCTGAAGATTTTATGGA+AGG | - | chr7.2:4993760-4993779 | MS.gene28654:CDS | 25.0% |
| CTAAGAAGAGAAAGGTTAAT+GGG | - | chr7.2:4994218-4994237 | MS.gene28654:CDS | 30.0% | |
| GAAGAACATTCATTTGTTTG+AGG | + | chr7.2:4994064-4994083 | None:intergenic | 30.0% | |
| GTTTATGAAGAGAAAGATCT+TGG | + | chr7.2:4993916-4993935 | None:intergenic | 30.0% | |
| TAATCGGAAGAAGATTGATT+TGG | - | chr7.2:4994453-4994472 | MS.gene28654:CDS | 30.0% | |
| ! | ATGATGATGTAATAGGTTGT+TGG | + | chr7.2:4994015-4994034 | None:intergenic | 30.0% |
| !! | GTTGTTGATGATGATGTAAT+AGG | + | chr7.2:4994022-4994041 | None:intergenic | 30.0% |
| !!! | AGAAAGTATAGACTTTTTCG+AGG | - | chr7.2:4994376-4994395 | MS.gene28654:CDS | 30.0% |
| AGGGCTTATGTTTGTGATAA+TGG | - | chr7.2:4994574-4994593 | MS.gene28654:CDS | 35.0% | |
| ATTTCAAGAAGACACGTGTT+TGG | - | chr7.2:4994410-4994429 | MS.gene28654:CDS | 35.0% | |
| CACATTTAGCTACAGGAAAA+TGG | - | chr7.2:4994251-4994270 | MS.gene28654:CDS | 35.0% | |
| GAACATTCATTTGTTTGAGG+TGG | + | chr7.2:4994061-4994080 | None:intergenic | 35.0% | |
| GAAGAAAGAAGAAGTTCAGA+AGG | + | chr7.2:4993890-4993909 | None:intergenic | 35.0% | |
| GCTAAGAAGAGAAAGGTTAA+TGG | - | chr7.2:4994217-4994236 | MS.gene28654:CDS | 35.0% | |
| TGTTCTTCCAACAGAAATCA+TGG | + | chr7.2:4993850-4993869 | None:intergenic | 35.0% | |
| TTGAGTTCACATTTAGCTAC+AGG | - | chr7.2:4994244-4994263 | MS.gene28654:CDS | 35.0% | |
| ! | GATGAGAGAATTGAGTTTTG+TGG | + | chr7.2:4993828-4993847 | None:intergenic | 35.0% |
| ! | GGATTTGGTTATCATGATGA+TGG | - | chr7.2:4994538-4994557 | MS.gene28654:CDS | 35.0% |
| !! | TGTGATAATGGTCATGTTTC+TGG | - | chr7.2:4994586-4994605 | MS.gene28654:CDS | 35.0% |
| !! | TTTTGTTTGAGGAGAGGATT+TGG | - | chr7.2:4994523-4994542 | MS.gene28654:CDS | 35.0% |
| !!! | TTCACTCTGCTTTTTGTTTG+AGG | - | chr7.2:4994512-4994531 | MS.gene28654:CDS | 35.0% |
| AACGAGGAAGAGATTAGAAC+TGG | - | chr7.2:4994172-4994191 | MS.gene28654:CDS | 40.0% | |
| AAGAGAATACCAGCATGAAG+AGG | + | chr7.2:4993959-4993978 | None:intergenic | 40.0% | |
| ACCAGAAGCTAAGAAGAGAA+AGG | - | chr7.2:4994210-4994229 | MS.gene28654:CDS | 40.0% | |
| ACCTTTCTCTTCTTAGCTTC+TGG | + | chr7.2:4994214-4994233 | None:intergenic | 40.0% | |
| CAAACATAAGCCCTAACAAC+AGG | + | chr7.2:4994568-4994587 | None:intergenic | 40.0% | |
| CTCTCATCCATGATTTCTGT+TGG | - | chr7.2:4993840-4993859 | MS.gene28654:CDS | 40.0% | |
| GAAGCTCATCATCTTCAACA+GGG | - | chr7.2:4994136-4994155 | MS.gene28654:CDS | 40.0% | |
| TCTTCCAAATGAACACAACC+TGG | + | chr7.2:4994352-4994371 | None:intergenic | 40.0% | |
| TGAAGCTCATCATCTTCAAC+AGG | - | chr7.2:4994135-4994154 | MS.gene28654:CDS | 40.0% | |
| TGGACTCTTAGTAGAGAACA+AGG | - | chr7.2:4994271-4994290 | MS.gene28654:CDS | 40.0% | |
| TTGTTCATCTGGGAAATCAG+CGG | + | chr7.2:4993987-4994006 | None:intergenic | 40.0% | |
| TTTCTCTTCTTAGCTTCTGG+TGG | + | chr7.2:4994211-4994230 | None:intergenic | 40.0% | |
| ! | AGTTCTAATCTCTTCCTCGT+TGG | + | chr7.2:4994173-4994192 | None:intergenic | 40.0% |
| ! | ATGATGGTGAACCTGTTGTT+AGG | - | chr7.2:4994554-4994573 | MS.gene28654:CDS | 40.0% |
| ! | TGATGGTGAACCTGTTGTTA+GGG | - | chr7.2:4994555-4994574 | MS.gene28654:CDS | 40.0% |
| ! | TTATACGTTTGTGATTGGCC+AGG | - | chr7.2:4994331-4994350 | MS.gene28654:CDS | 40.0% |
| !! | GTTGGTGTTGTTGTTCATCT+GGG | + | chr7.2:4993997-4994016 | None:intergenic | 40.0% |
| !! | TGTTGGTGTTGTTGTTCATC+TGG | + | chr7.2:4993998-4994017 | None:intergenic | 40.0% |
| !!! | TCTGCTTTTTGTTTGAGGAG+AGG | - | chr7.2:4994517-4994536 | MS.gene28654:CDS | 40.0% |
| AAACTACCCTTCTGTTGTCC+TGG | - | chr7.2:4993932-4993951 | MS.gene28654:CDS | 45.0% | |
| TGAAGAAGACCCTCGTGATT+GGG | - | chr7.2:4993784-4993803 | MS.gene28654:CDS | 45.0% | |
| TGAGGTGGAAGAGATGATGA+AGG | + | chr7.2:4994046-4994065 | None:intergenic | 45.0% | |
| TTGAAGAAGACCCTCGTGAT+TGG | - | chr7.2:4993783-4993802 | MS.gene28654:CDS | 45.0% | |
| TTGGCCAGGTTGTGTTCATT+TGG | - | chr7.2:4994345-4994364 | MS.gene28654:CDS | 45.0% | |
| !! | ATGGTCATGTTTCTGGTGCT+TGG | - | chr7.2:4994593-4994612 | MS.gene28654:CDS | 45.0% |
| AAAGACGAGCCCAATCACGA+GGG | + | chr7.2:4993796-4993815 | None:intergenic | 50.0% | |
| AAGAGGACCAGGACAACAGA+AGG | + | chr7.2:4993942-4993961 | None:intergenic | 50.0% | |
| AGAGGACCAGGACAACAGAA+GGG | + | chr7.2:4993941-4993960 | None:intergenic | 50.0% | |
| ATACCAGCATGAAGAGGACC+AGG | + | chr7.2:4993953-4993972 | None:intergenic | 50.0% | |
| GAAAGACGAGCCCAATCACG+AGG | + | chr7.2:4993797-4993816 | None:intergenic | 55.0% | |
| GGGACAAGAACAGACCAACG+AGG | - | chr7.2:4994156-4994175 | MS.gene28654:CDS | 55.0% | |
| TGTCCTGGTCCTCTTCATGC+TGG | - | chr7.2:4993947-4993966 | MS.gene28654:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 4993734 | 4994639 | 4993734 | ID=MS.gene28654 |
| chr7.2 | mRNA | 4993734 | 4994639 | 4993734 | ID=MS.gene28654.t1;Parent=MS.gene28654 |
| chr7.2 | exon | 4993734 | 4994639 | 4993734 | ID=MS.gene28654.t1.exon1;Parent=MS.gene28654.t1 |
| chr7.2 | CDS | 4993734 | 4994639 | 4993734 | ID=cds.MS.gene28654.t1;Parent=MS.gene28654.t1 |
| Gene Sequence |
| Protein sequence |
