Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28722.t1 | XP_013457293.1 | 95.2 | 543 | 26 | 0 | 1 | 543 | 1 | 543 | 1.90E-304 | 1054.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28722.t1 | Q9SA85 | 54.6 | 533 | 223 | 7 | 8 | 534 | 7 | 526 | 1.9e-167 | 590.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28722.t1 | A0A072UNE4 | 95.2 | 543 | 26 | 0 | 1 | 543 | 1 | 543 | 1.4e-304 | 1054.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054576 | MS.gene28722 | 0.824784 | 6.69E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene28722 | 0.822389 | 2.43E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene28722 | 0.827027 | 1.96E-54 | -1.69E-46 |
| MS.gene060840 | MS.gene28722 | 0.802007 | 6.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28722.t1 | MTR_4g094498 | 95.212 | 543 | 26 | 0 | 1 | 543 | 1 | 543 | 0.0 | 1014 |
| MS.gene28722.t1 | MTR_2g031560 | 59.642 | 503 | 200 | 2 | 36 | 535 | 22 | 524 | 0.0 | 643 |
| MS.gene28722.t1 | MTR_2g031590 | 59.604 | 505 | 197 | 2 | 37 | 534 | 28 | 532 | 0.0 | 627 |
| MS.gene28722.t1 | MTR_4g094515 | 57.769 | 502 | 205 | 3 | 38 | 533 | 39 | 539 | 0.0 | 593 |
| MS.gene28722.t1 | MTR_4g094515 | 55.159 | 533 | 236 | 1 | 9 | 538 | 622 | 1154 | 0.0 | 593 |
| MS.gene28722.t1 | MTR_2g031490 | 56.530 | 536 | 220 | 6 | 7 | 534 | 8 | 538 | 0.0 | 592 |
| MS.gene28722.t1 | MTR_2g031510 | 57.822 | 505 | 202 | 5 | 38 | 534 | 39 | 540 | 0.0 | 584 |
| MS.gene28722.t1 | MTR_2g031390 | 52.894 | 501 | 230 | 3 | 39 | 534 | 30 | 529 | 0.0 | 562 |
| MS.gene28722.t1 | MTR_4g094495 | 54.008 | 524 | 232 | 3 | 23 | 537 | 20 | 543 | 0.0 | 556 |
| MS.gene28722.t1 | MTR_4g094480 | 54.004 | 537 | 238 | 5 | 8 | 537 | 4 | 538 | 0.0 | 553 |
| MS.gene28722.t1 | MTR_4g094478 | 53.458 | 535 | 238 | 5 | 8 | 534 | 4 | 535 | 0.0 | 548 |
| MS.gene28722.t1 | MTR_4g094492 | 52.788 | 538 | 244 | 6 | 8 | 537 | 4 | 539 | 0.0 | 547 |
| MS.gene28722.t1 | MTR_4g094488 | 53.922 | 510 | 226 | 3 | 37 | 537 | 31 | 540 | 0.0 | 546 |
| MS.gene28722.t1 | MTR_4g094518 | 53.770 | 504 | 227 | 3 | 36 | 534 | 28 | 530 | 0.0 | 544 |
| MS.gene28722.t1 | MTR_4g088355 | 55.754 | 504 | 218 | 4 | 39 | 537 | 33 | 536 | 0.0 | 543 |
| MS.gene28722.t1 | MTR_4g091150 | 55.357 | 504 | 215 | 5 | 39 | 534 | 39 | 540 | 0.0 | 541 |
| MS.gene28722.t1 | MTR_3g009820 | 50.568 | 528 | 248 | 5 | 12 | 536 | 7 | 524 | 0.0 | 537 |
| MS.gene28722.t1 | MTR_3g009880 | 50.662 | 529 | 249 | 5 | 11 | 536 | 3 | 522 | 0.0 | 536 |
| MS.gene28722.t1 | MTR_4g091200 | 53.578 | 517 | 223 | 5 | 21 | 533 | 22 | 525 | 0.0 | 532 |
| MS.gene28722.t1 | MTR_3g009760 | 50.000 | 530 | 248 | 6 | 12 | 536 | 7 | 524 | 0.0 | 531 |
| MS.gene28722.t1 | MTR_2g031420 | 53.785 | 502 | 225 | 4 | 37 | 535 | 31 | 528 | 0.0 | 531 |
| MS.gene28722.t1 | MTR_4g094520 | 50.564 | 532 | 249 | 5 | 7 | 534 | 11 | 532 | 0.0 | 530 |
| MS.gene28722.t1 | MTR_2g031460 | 52.953 | 508 | 232 | 4 | 37 | 541 | 31 | 534 | 0.0 | 526 |
| MS.gene28722.t1 | MTR_4g091190 | 50.662 | 529 | 258 | 3 | 14 | 540 | 7 | 534 | 0.0 | 523 |
| MS.gene28722.t1 | MTR_6g017205 | 52.713 | 516 | 227 | 5 | 22 | 533 | 23 | 525 | 0.0 | 523 |
| MS.gene28722.t1 | MTR_4g057990 | 50.476 | 525 | 248 | 6 | 11 | 533 | 7 | 521 | 2.40e-175 | 505 |
| MS.gene28722.t1 | MTR_4g094418 | 57.569 | 436 | 175 | 4 | 110 | 535 | 1 | 436 | 7.28e-175 | 507 |
| MS.gene28722.t1 | MTR_2g023590 | 52.790 | 466 | 218 | 2 | 73 | 536 | 3 | 468 | 4.97e-166 | 479 |
| MS.gene28722.t1 | MTR_4g091170 | 49.672 | 457 | 210 | 3 | 80 | 533 | 102 | 541 | 2.09e-157 | 461 |
| MS.gene28722.t1 | MTR_1g076830 | 42.020 | 495 | 274 | 5 | 50 | 531 | 38 | 532 | 1.88e-127 | 384 |
| MS.gene28722.t1 | MTR_2g031600 | 66.667 | 216 | 72 | 0 | 37 | 252 | 29 | 244 | 1.08e-95 | 297 |
| MS.gene28722.t1 | MTR_2g031600 | 59.236 | 157 | 64 | 0 | 378 | 534 | 244 | 400 | 1.30e-63 | 213 |
| MS.gene28722.t1 | MTR_3g009850 | 51.082 | 231 | 112 | 1 | 308 | 537 | 1 | 231 | 6.09e-76 | 240 |
| MS.gene28722.t1 | MTR_2g084370 | 43.243 | 111 | 41 | 2 | 134 | 244 | 1 | 89 | 3.22e-27 | 105 |
| MS.gene28722.t1 | MTR_2g084405 | 46.154 | 91 | 49 | 0 | 134 | 224 | 1 | 91 | 1.75e-19 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28722.t1 | AT4G20840 | 55.865 | 503 | 218 | 3 | 38 | 537 | 37 | 538 | 0.0 | 566 |
| MS.gene28722.t1 | AT1G30700 | 54.597 | 533 | 223 | 7 | 8 | 534 | 7 | 526 | 0.0 | 559 |
| MS.gene28722.t1 | AT1G34575 | 54.095 | 525 | 224 | 7 | 12 | 532 | 11 | 522 | 0.0 | 559 |
| MS.gene28722.t1 | AT1G01980 | 54.706 | 510 | 222 | 5 | 38 | 539 | 31 | 539 | 0.0 | 556 |
| MS.gene28722.t1 | AT1G11770 | 53.307 | 499 | 229 | 3 | 39 | 534 | 32 | 529 | 0.0 | 550 |
| MS.gene28722.t1 | AT4G20830 | 55.952 | 504 | 218 | 3 | 37 | 537 | 37 | 539 | 0.0 | 548 |
| MS.gene28722.t1 | AT4G20830 | 55.952 | 504 | 218 | 3 | 37 | 537 | 37 | 539 | 0.0 | 548 |
| MS.gene28722.t1 | AT1G30710 | 54.871 | 503 | 214 | 6 | 41 | 536 | 34 | 530 | 0.0 | 548 |
| MS.gene28722.t1 | AT5G44400 | 49.159 | 535 | 256 | 7 | 7 | 534 | 11 | 536 | 0.0 | 524 |
| MS.gene28722.t1 | AT1G30760 | 51.195 | 502 | 233 | 5 | 39 | 533 | 35 | 531 | 8.15e-180 | 517 |
| MS.gene28722.t1 | AT1G30760 | 51.195 | 502 | 233 | 5 | 39 | 533 | 53 | 549 | 8.60e-180 | 518 |
| MS.gene28722.t1 | AT2G34790 | 49.434 | 530 | 249 | 7 | 11 | 533 | 12 | 529 | 2.10e-179 | 516 |
| MS.gene28722.t1 | AT1G26390 | 50.501 | 499 | 240 | 4 | 39 | 532 | 29 | 525 | 5.39e-179 | 515 |
| MS.gene28722.t1 | AT1G30720 | 50.685 | 511 | 246 | 4 | 26 | 532 | 16 | 524 | 8.81e-178 | 511 |
| MS.gene28722.t1 | AT1G26380 | 51.190 | 504 | 239 | 5 | 39 | 538 | 29 | 529 | 1.42e-177 | 511 |
| MS.gene28722.t1 | AT1G26420 | 50.095 | 525 | 248 | 8 | 13 | 532 | 8 | 523 | 1.75e-177 | 511 |
| MS.gene28722.t1 | AT1G30740 | 50.095 | 529 | 258 | 4 | 11 | 534 | 1 | 528 | 8.45e-177 | 509 |
| MS.gene28722.t1 | AT1G30740 | 50.095 | 529 | 258 | 4 | 11 | 534 | 4 | 531 | 9.25e-177 | 509 |
| MS.gene28722.t1 | AT1G30730 | 49.053 | 528 | 255 | 6 | 11 | 532 | 4 | 523 | 1.25e-176 | 509 |
| MS.gene28722.t1 | AT1G26400 | 51.116 | 493 | 235 | 5 | 42 | 531 | 32 | 521 | 2.93e-176 | 508 |
| MS.gene28722.t1 | AT1G26410 | 50.000 | 500 | 239 | 6 | 39 | 532 | 53 | 547 | 9.56e-175 | 505 |
| MS.gene28722.t1 | AT4G20800 | 50.499 | 501 | 243 | 3 | 39 | 536 | 29 | 527 | 9.39e-174 | 501 |
| MS.gene28722.t1 | AT5G44390 | 50.000 | 508 | 244 | 7 | 36 | 534 | 34 | 540 | 1.26e-173 | 501 |
| MS.gene28722.t1 | AT4G20820 | 48.302 | 530 | 258 | 7 | 6 | 532 | 8 | 524 | 5.37e-170 | 492 |
| MS.gene28722.t1 | AT5G44380 | 48.964 | 531 | 255 | 7 | 11 | 534 | 18 | 539 | 1.68e-168 | 488 |
| MS.gene28722.t1 | AT5G44380 | 48.964 | 531 | 255 | 7 | 11 | 534 | 58 | 579 | 1.99e-168 | 490 |
| MS.gene28722.t1 | AT2G34810 | 49.213 | 508 | 245 | 6 | 39 | 535 | 35 | 540 | 8.62e-165 | 479 |
| MS.gene28722.t1 | AT4G20860 | 49.241 | 461 | 229 | 3 | 75 | 534 | 73 | 529 | 5.33e-162 | 471 |
| MS.gene28722.t1 | AT5G44360 | 47.417 | 542 | 265 | 11 | 1 | 534 | 1 | 530 | 5.17e-161 | 469 |
| MS.gene28722.t1 | AT5G44360 | 47.417 | 542 | 265 | 11 | 1 | 534 | 17 | 546 | 5.49e-161 | 469 |
| MS.gene28722.t1 | AT5G44440 | 47.358 | 530 | 262 | 8 | 12 | 532 | 6 | 527 | 7.01e-161 | 469 |
| MS.gene28722.t1 | AT5G44440 | 47.358 | 530 | 262 | 8 | 12 | 532 | 76 | 597 | 9.82e-160 | 468 |
| MS.gene28722.t1 | AT5G44410 | 47.103 | 535 | 259 | 10 | 8 | 532 | 7 | 527 | 2.58e-150 | 442 |
Find 112 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCTGATGTAGGAGTTTA+AGG | 0.141806 | 4.3:+19428431 | None:intergenic |
| GGTAACATGATGAGAAAATT+TGG | 0.203065 | 4.3:-19428048 | MS.gene28722:CDS |
| AAATTCAGTACTCAGTAAAT+TGG | 0.225006 | 4.3:-19427330 | MS.gene28722:CDS |
| GTTATCTGTTGATAATATTT+TGG | 0.226666 | 4.3:-19428025 | MS.gene28722:CDS |
| TGATGTTGCGAATCAATATT+TGG | 0.237497 | 4.3:-19427293 | MS.gene28722:CDS |
| CAGAAGCCGATATCGAAAAT+TGG | 0.245756 | 4.3:-19427484 | MS.gene28722:CDS |
| AAGTTCAATGCTTTGTTTCT+TGG | 0.250620 | 4.3:-19427703 | MS.gene28722:CDS |
| GTTATGAAGAAGCTAGTATT+TGG | 0.256401 | 4.3:-19427156 | MS.gene28722:CDS |
| CACTTCTGATTCTGATTTCA+AGG | 0.264026 | 4.3:+19428307 | None:intergenic |
| ACATTGGAGAAGGGTATTAT+TGG | 0.289591 | 4.3:+19428530 | None:intergenic |
| TCACTCACATTCAATCCTTA+TGG | 0.296274 | 4.3:-19427418 | MS.gene28722:CDS |
| CAATTGGTGAAGTTTATTAT+AGG | 0.302894 | 4.3:-19428158 | MS.gene28722:CDS |
| GTGAGAATGAAAACGGGTTT+TGG | 0.306146 | 4.3:+19428384 | None:intergenic |
| GCTGAGATTAGTAGTGTTCA+TGG | 0.313039 | 4.3:-19428132 | MS.gene28722:CDS |
| TTCTTCCCCTTTCTCTTAGC+AGG | 0.322817 | 4.3:+19427739 | None:intergenic |
| TTATGAAGAAGCTAGTATTT+GGG | 0.337096 | 4.3:-19427155 | MS.gene28722:CDS |
| TTGATGGAAGGATTCTTGAT+AGG | 0.341832 | 4.3:-19427984 | MS.gene28722:CDS |
| AAATTGGGTTGGATGGTATA+TGG | 0.351960 | 4.3:-19427468 | MS.gene28722:CDS |
| TAGTGGATGAGTTGAATCTA+AGG | 0.353487 | 4.3:+19428409 | None:intergenic |
| GGATTGATTCAGTGTTGTTT+TGG | 0.358314 | 4.3:-19427597 | MS.gene28722:CDS |
| GGTGGTGGTGGAGCTAGTTT+TGG | 0.359729 | 4.3:-19427925 | MS.gene28722:CDS |
| TAGTTCACATACGAACTTCT+TGG | 0.361444 | 4.3:+19427220 | None:intergenic |
| GGTGAATATGAAGGTGTGTT+TGG | 0.363695 | 4.3:+19428456 | None:intergenic |
| ACTTCTGAGATTGGATTAGA+AGG | 0.364151 | 4.3:+19428489 | None:intergenic |
| ATCAATATTTGGATCGGATT+CGG | 0.364710 | 4.3:-19427282 | MS.gene28722:CDS |
| AAAGGGGAAGAAAACAATTA+AGG | 0.365322 | 4.3:-19427728 | MS.gene28722:CDS |
| GTAACATGATGAGAAAATTT+GGG | 0.371973 | 4.3:-19428047 | MS.gene28722:CDS |
| TCACCAATCAAACCCAATTC+AGG | 0.380665 | 4.3:+19427640 | None:intergenic |
| CTTGGTGAATTTGACACATA+AGG | 0.383022 | 4.3:+19427238 | None:intergenic |
| ATTTCAAAGGAAACTTTGAT+AGG | 0.386790 | 4.3:-19427123 | MS.gene28722:CDS |
| GCTAAGATTGTTGATGTTGA+TGG | 0.387710 | 4.3:-19428000 | MS.gene28722:CDS |
| TTTCCTGAATTGGGTTTGAT+TGG | 0.401805 | 4.3:-19427643 | MS.gene28722:CDS |
| AGATGGGGTGAGAATGAAAA+CGG | 0.420577 | 4.3:+19428377 | None:intergenic |
| GAATCTTTGTGGGTTGAATC+AGG | 0.425783 | 4.3:-19428186 | MS.gene28722:CDS |
| TGAAGGTGTGTTTGGGAAGT+AGG | 0.431103 | 4.3:+19428464 | None:intergenic |
| GTTTGTCCTACTGTTGGTGT+TGG | 0.433697 | 4.3:-19428096 | MS.gene28722:CDS |
| AAGTAGGTAACTTCTGAGAT+TGG | 0.442446 | 4.3:+19428480 | None:intergenic |
| TGGGCTATTAGAGGTGGTGG+TGG | 0.463521 | 4.3:-19427937 | MS.gene28722:CDS |
| GAATCTAAGGTTTCTGATGT+AGG | 0.467537 | 4.3:+19428422 | None:intergenic |
| TGTCCTACTGTTGGTGTTGG+TGG | 0.469479 | 4.3:-19428093 | MS.gene28722:CDS |
| TGTTTCTTGGAAGATTAAGT+TGG | 0.470889 | 4.3:-19427896 | MS.gene28722:CDS |
| TGGTATTAATATCATTATGT+TGG | 0.473730 | 4.3:+19428550 | None:intergenic |
| GTGAATATGAAGGTGTGTTT+GGG | 0.475111 | 4.3:+19428457 | None:intergenic |
| GTTAGTCCTGCTAAGAGAAA+GGG | 0.476006 | 4.3:-19427745 | MS.gene28722:CDS |
| GATGTTGATATAGGTGTCAA+TGG | 0.476097 | 4.3:-19427193 | MS.gene28722:CDS |
| TATAAAAGATGAATCTTTGT+GGG | 0.478869 | 4.3:-19428196 | MS.gene28722:CDS |
| AGAAGCCGATATCGAAAATT+GGG | 0.479345 | 4.3:-19427483 | MS.gene28722:CDS |
| CTGATGTAGGAGTTTAAGGT+GGG | 0.479655 | 4.3:+19428435 | None:intergenic |
| CCCATCAAGATGCATACAAT+TGG | 0.482333 | 4.3:-19428642 | None:intergenic |
| AGAGAGAAAGACATTGGAGA+AGG | 0.488844 | 4.3:+19428520 | None:intergenic |
| TCTGATGTAGGAGTTTAAGG+TGG | 0.489776 | 4.3:+19428434 | None:intergenic |
| TGTTAGTCCTGCTAAGAGAA+AGG | 0.490473 | 4.3:-19427746 | MS.gene28722:CDS |
| GATTCTTGATAGGGAATCAA+TGG | 0.496588 | 4.3:-19427974 | MS.gene28722:CDS |
| GAACATGTCAAGAACAATGA+AGG | 0.496807 | 4.3:+19428242 | None:intergenic |
| TGATGGAAGGATTCTTGATA+GGG | 0.500795 | 4.3:-19427983 | MS.gene28722:CDS |
| ATATAAAAGATGAATCTTTG+TGG | 0.502433 | 4.3:-19428197 | MS.gene28722:CDS |
| AGCAATGTATTGAAATGAGT+TGG | 0.506091 | 4.3:-19427618 | MS.gene28722:CDS |
| ATGTGGAATACTATGAAGAA+TGG | 0.513188 | 4.3:+19428606 | None:intergenic |
| ACAGTAGGACAAACCCCAGC+TGG | 0.514116 | 4.3:+19428105 | None:intergenic |
| GCTGGGGTTTGTCCTACTGT+TGG | 0.516701 | 4.3:-19428102 | MS.gene28722:CDS |
| TGCATCTGAGATTAGTGTAA+TGG | 0.517443 | 4.3:-19427032 | MS.gene28722:CDS |
| GAGAGAAAGACATTGGAGAA+GGG | 0.518172 | 4.3:+19428521 | None:intergenic |
| CATGTTCTTGCTTAGATCAG+TGG | 0.519701 | 4.3:-19428226 | MS.gene28722:CDS |
| AACATGTTACCAGCTCTATG+TGG | 0.525316 | 4.3:+19427355 | None:intergenic |
| TGCAGCATATGATTGATGCT+TGG | 0.525800 | 4.3:+19428340 | None:intergenic |
| GATGGGGTGAGAATGAAAAC+GGG | 0.526268 | 4.3:+19428378 | None:intergenic |
| TTTAAGGTGGGTGAATATGA+AGG | 0.526473 | 4.3:+19428447 | None:intergenic |
| TTGCGAATCAATATTTGGAT+CGG | 0.526644 | 4.3:-19427288 | MS.gene28722:CDS |
| ATTCTTGATAGGGAATCAAT+GGG | 0.530524 | 4.3:-19427973 | MS.gene28722:CDS |
| CTCACATTCAATCCTTATGG+TGG | 0.534887 | 4.3:-19427415 | MS.gene28722:CDS |
| TGGAAGAAGATGATTGAACT+AGG | 0.545464 | 4.3:-19427448 | MS.gene28722:CDS |
| GATATAGGTGTCAATGGAGA+GGG | 0.546721 | 4.3:-19427187 | MS.gene28722:CDS |
| GTACTCAGTAAATTGGAAAG+AGG | 0.547618 | 4.3:-19427323 | MS.gene28722:CDS |
| AACTATAGAGATGTTGATAT+AGG | 0.549791 | 4.3:-19427202 | MS.gene28722:CDS |
| GCCGATATCGAAAATTGGGT+TGG | 0.554890 | 4.3:-19427479 | MS.gene28722:CDS |
| GTTGAATCAGGTGCTACAAT+TGG | 0.555094 | 4.3:-19428174 | MS.gene28722:CDS |
| ATATCGAAAATTGGGTTGGA+TGG | 0.562553 | 4.3:-19427475 | MS.gene28722:CDS |
| CAAAAGAGAAGTTCTTGAAG+AGG | 0.567801 | 4.3:-19427522 | MS.gene28722:CDS |
| CTGAATAGAGAGAAAGACAT+TGG | 0.568278 | 4.3:+19428514 | None:intergenic |
| GGATGTGAGAGATATGAGAT+GGG | 0.576189 | 4.3:+19428361 | None:intergenic |
| AATGAGTGAGATTTCTGAGG+TGG | 0.577760 | 4.3:-19427389 | MS.gene28722:CDS |
| CTAATCTCAGATGCAAGTGA+TGG | 0.584295 | 4.3:+19427040 | None:intergenic |
| TTACCAGCTCTATGTGGGAA+TGG | 0.586583 | 4.3:+19427361 | None:intergenic |
| AAACTTTGATAGGCTTGTAG+AGG | 0.593703 | 4.3:-19427113 | MS.gene28722:CDS |
| GTTGCTGATAAGATACATGA+TGG | 0.594648 | 4.3:-19427787 | MS.gene28722:CDS |
| ATATCAGTCGAAGTTCCTAC+CGG | 0.604560 | 4.3:+19427565 | None:intergenic |
| AGATTGTTGATGTTGATGGA+AGG | 0.611403 | 4.3:-19427996 | MS.gene28722:CDS |
| ACACCATTCCCACATAGAGC+TGG | 0.611903 | 4.3:-19427364 | MS.gene28722:CDS |
| ATATAGGTGTCAATGGAGAG+GGG | 0.613762 | 4.3:-19427186 | MS.gene28722:CDS |
| AAGACACTGGTACTAGAATG+TGG | 0.614563 | 4.3:+19428589 | None:intergenic |
| AACATGTCAAGAACAATGAA+GGG | 0.614696 | 4.3:+19428243 | None:intergenic |
| TCACATTCAATCCTTATGGT+GGG | 0.617770 | 4.3:-19427414 | MS.gene28722:CDS |
| TATGAAGAAGCTAGTATTTG+GGG | 0.618664 | 4.3:-19427154 | MS.gene28722:CDS |
| GTGGTGATGTTGTTCATCAG+TGG | 0.621789 | 4.3:-19427816 | MS.gene28722:CDS |
| GAAATCAGAATCAGAAGTGG+TGG | 0.623397 | 4.3:-19428303 | MS.gene28722:CDS |
| ACATGTTACCAGCTCTATGT+GGG | 0.626075 | 4.3:+19427356 | None:intergenic |
| CTTGAAATCAGAATCAGAAG+TGG | 0.627667 | 4.3:-19428306 | MS.gene28722:CDS |
| TACTATGAAGAATGGAACAA+GGG | 0.629238 | 4.3:+19428614 | None:intergenic |
| TTAGTCCTGCTAAGAGAAAG+GGG | 0.631092 | 4.3:-19427744 | MS.gene28722:CDS |
| TATAGGTGTCAATGGAGAGG+GGG | 0.633068 | 4.3:-19427185 | MS.gene28722:CDS |
| ATACTATGAAGAATGGAACA+AGG | 0.635254 | 4.3:+19428613 | None:intergenic |
| TGGATGTGAGAGATATGAGA+TGG | 0.636135 | 4.3:+19428360 | None:intergenic |
| TGATATAGGTGTCAATGGAG+AGG | 0.640685 | 4.3:-19427188 | MS.gene28722:CDS |
| TGTCCACCAACACCAACAGT+AGG | 0.646587 | 4.3:+19428090 | None:intergenic |
| AGTGGTGGTCATGACTATGA+TGG | 0.659640 | 4.3:-19428288 | MS.gene28722:CDS |
| AAAACATTGGAACAAGGTGG+TGG | 0.663778 | 4.3:-19427835 | MS.gene28722:CDS |
| TCTTGATAGGGAATCAATGG+GGG | 0.665119 | 4.3:-19427971 | MS.gene28722:CDS |
| TTCTTGATAGGGAATCAATG+GGG | 0.670213 | 4.3:-19427972 | MS.gene28722:CDS |
| ACAGAATCTGAAGAAGACAC+TGG | 0.698244 | 4.3:+19428576 | None:intergenic |
| GAAAATGAGTGAGATTTCTG+AGG | 0.702809 | 4.3:-19427392 | MS.gene28722:CDS |
| TTGCTGATAAGATACATGAT+GGG | 0.706046 | 4.3:-19427786 | MS.gene28722:CDS |
| GATGTGAGAGATATGAGATG+GGG | 0.755774 | 4.3:+19428362 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAAAGATGAATCTTTG+TGG | - | chr4.3:19427460-19427479 | MS.gene28722:CDS | 20.0% |
| !! | TATAAAAGATGAATCTTTGT+GGG | - | chr4.3:19427461-19427480 | MS.gene28722:CDS | 20.0% |
| !! | TCATATTTGAAAAAATTGCT+TGG | + | chr4.3:19428587-19428606 | None:intergenic | 20.0% |
| !! | TGGTATTAATATCATTATGT+TGG | + | chr4.3:19427110-19427129 | None:intergenic | 20.0% |
| !!! | GTTATCTGTTGATAATATTT+TGG | - | chr4.3:19427632-19427651 | MS.gene28722:CDS | 20.0% |
| !!! | TTTTAGAGTTGAAAAAACAT+TGG | - | chr4.3:19427809-19427828 | MS.gene28722:CDS | 20.0% |
| ! | AAATTCAGTACTCAGTAAAT+TGG | - | chr4.3:19428327-19428346 | MS.gene28722:CDS | 25.0% |
| ! | AACTATAGAGATGTTGATAT+AGG | - | chr4.3:19428455-19428474 | MS.gene28722:CDS | 25.0% |
| ! | ATTTCAAAGGAAACTTTGAT+AGG | - | chr4.3:19428534-19428553 | MS.gene28722:CDS | 25.0% |
| ! | CAATTGGTGAAGTTTATTAT+AGG | - | chr4.3:19427499-19427518 | MS.gene28722:CDS | 25.0% |
| ! | GTAACATGATGAGAAAATTT+GGG | - | chr4.3:19427610-19427629 | MS.gene28722:CDS | 25.0% |
| ! | TTATGAAGAAGCTAGTATTT+GGG | - | chr4.3:19428502-19428521 | MS.gene28722:CDS | 25.0% |
| AAAGGGGAAGAAAACAATTA+AGG | - | chr4.3:19427929-19427948 | MS.gene28722:CDS | 30.0% | |
| AACATGTCAAGAACAATGAA+GGG | + | chr4.3:19427417-19427436 | None:intergenic | 30.0% | |
| AGCAATGTATTGAAATGAGT+TGG | - | chr4.3:19428039-19428058 | MS.gene28722:CDS | 30.0% | |
| ATACTATGAAGAATGGAACA+AGG | + | chr4.3:19427047-19427066 | None:intergenic | 30.0% | |
| ATCAATATTTGGATCGGATT+CGG | - | chr4.3:19428375-19428394 | MS.gene28722:CDS | 30.0% | |
| ATGTGGAATACTATGAAGAA+TGG | + | chr4.3:19427054-19427073 | None:intergenic | 30.0% | |
| ATTCTTGATAGGGAATCAAT+GGG | - | chr4.3:19427684-19427703 | MS.gene28722:CDS | 30.0% | |
| CTAAAAACAGTTACAATCTC+AGG | + | chr4.3:19427795-19427814 | None:intergenic | 30.0% | |
| GGTAACATGATGAGAAAATT+TGG | - | chr4.3:19427609-19427628 | MS.gene28722:CDS | 30.0% | |
| GTTATGAAGAAGCTAGTATT+TGG | - | chr4.3:19428501-19428520 | MS.gene28722:CDS | 30.0% | |
| GTTGAAAAAACATTGGAACA+AGG | - | chr4.3:19427816-19427835 | MS.gene28722:CDS | 30.0% | |
| TACTATGAAGAATGGAACAA+GGG | + | chr4.3:19427046-19427065 | None:intergenic | 30.0% | |
| TATGAAGAAGCTAGTATTTG+GGG | - | chr4.3:19428503-19428522 | MS.gene28722:CDS | 30.0% | |
| TGATGTTGCGAATCAATATT+TGG | - | chr4.3:19428364-19428383 | MS.gene28722:CDS | 30.0% | |
| TGTTTCTTGGAAGATTAAGT+TGG | - | chr4.3:19427761-19427780 | MS.gene28722:CDS | 30.0% | |
| TTGCGAATCAATATTTGGAT+CGG | - | chr4.3:19428369-19428388 | MS.gene28722:CDS | 30.0% | |
| TTGCTGATAAGATACATGAT+GGG | - | chr4.3:19427871-19427890 | MS.gene28722:CDS | 30.0% | |
| ! | AATGAGAGTTTTCCTGAATT+GGG | - | chr4.3:19428005-19428024 | MS.gene28722:CDS | 30.0% |
| !! | AAGTTCAATGCTTTGTTTCT+TGG | - | chr4.3:19427954-19427973 | MS.gene28722:CDS | 30.0% |
| !! | TGGGGTAAAAAGTATTTCAA+AGG | - | chr4.3:19428521-19428540 | MS.gene28722:CDS | 30.0% |
| !!! | GTTGTTTTGGTATAACTATC+CGG | - | chr4.3:19428073-19428092 | MS.gene28722:CDS | 30.0% |
| !!! | GTTTTGGTGTTATTGTTTCT+TGG | - | chr4.3:19427748-19427767 | MS.gene28722:CDS | 30.0% |
| AAACTTTGATAGGCTTGTAG+AGG | - | chr4.3:19428544-19428563 | MS.gene28722:CDS | 35.0% | |
| AGAAGCCGATATCGAAAATT+GGG | - | chr4.3:19428174-19428193 | MS.gene28722:CDS | 35.0% | |
| CAAAAGAGAAGTTCTTGAAG+AGG | - | chr4.3:19428135-19428154 | MS.gene28722:CDS | 35.0% | |
| CACTTCTGATTCTGATTTCA+AGG | + | chr4.3:19427353-19427372 | None:intergenic | 35.0% | |
| CTGAATAGAGAGAAAGACAT+TGG | + | chr4.3:19427146-19427165 | None:intergenic | 35.0% | |
| CTTGAAATCAGAATCAGAAG+TGG | - | chr4.3:19427351-19427370 | MS.gene28722:CDS | 35.0% | |
| CTTGGTGAATTTGACACATA+AGG | + | chr4.3:19428422-19428441 | None:intergenic | 35.0% | |
| GAAAAAACATTGGAACAAGG+TGG | - | chr4.3:19427819-19427838 | MS.gene28722:CDS | 35.0% | |
| GAAAATGAGTGAGATTTCTG+AGG | - | chr4.3:19428265-19428284 | MS.gene28722:CDS | 35.0% | |
| GAACATGTCAAGAACAATGA+AGG | + | chr4.3:19427418-19427437 | None:intergenic | 35.0% | |
| GATGTTGATATAGGTGTCAA+TGG | - | chr4.3:19428464-19428483 | MS.gene28722:CDS | 35.0% | |
| GATTCTTGATAGGGAATCAA+TGG | - | chr4.3:19427683-19427702 | MS.gene28722:CDS | 35.0% | |
| GCTAAGATTGTTGATGTTGA+TGG | - | chr4.3:19427657-19427676 | MS.gene28722:CDS | 35.0% | |
| GTACTCAGTAAATTGGAAAG+AGG | - | chr4.3:19428334-19428353 | MS.gene28722:CDS | 35.0% | |
| GTTGCTGATAAGATACATGA+TGG | - | chr4.3:19427870-19427889 | MS.gene28722:CDS | 35.0% | |
| GTTTCTGATGTAGGAGTTTA+AGG | + | chr4.3:19427229-19427248 | None:intergenic | 35.0% | |
| TAGTTCACATACGAACTTCT+TGG | + | chr4.3:19428440-19428459 | None:intergenic | 35.0% | |
| TCACATTCAATCCTTATGGT+GGG | - | chr4.3:19428243-19428262 | MS.gene28722:CDS | 35.0% | |
| TCACTCACATTCAATCCTTA+TGG | - | chr4.3:19428239-19428258 | MS.gene28722:CDS | 35.0% | |
| TGATGGAAGGATTCTTGATA+GGG | - | chr4.3:19427674-19427693 | MS.gene28722:CDS | 35.0% | |
| TGGAAGAAGATGATTGAACT+AGG | - | chr4.3:19428209-19428228 | MS.gene28722:CDS | 35.0% | |
| TTCTTGATAGGGAATCAATG+GGG | - | chr4.3:19427685-19427704 | MS.gene28722:CDS | 35.0% | |
| TTGATGGAAGGATTCTTGAT+AGG | - | chr4.3:19427673-19427692 | MS.gene28722:CDS | 35.0% | |
| TTTCCTGAATTGGGTTTGAT+TGG | - | chr4.3:19428014-19428033 | MS.gene28722:CDS | 35.0% | |
| ! | AGATTGTTGATGTTGATGGA+AGG | - | chr4.3:19427661-19427680 | MS.gene28722:CDS | 35.0% |
| ! | CGACTGATATTTTGCTTGAA+AGG | - | chr4.3:19428105-19428124 | MS.gene28722:CDS | 35.0% |
| ! | GAATCTAAGGTTTCTGATGT+AGG | + | chr4.3:19427238-19427257 | None:intergenic | 35.0% |
| ! | GAATGAGAGTTTTCCTGAAT+TGG | - | chr4.3:19428004-19428023 | MS.gene28722:CDS | 35.0% |
| ! | GTGAATATGAAGGTGTGTTT+GGG | + | chr4.3:19427203-19427222 | None:intergenic | 35.0% |
| ! | TAGTGGATGAGTTGAATCTA+AGG | + | chr4.3:19427251-19427270 | None:intergenic | 35.0% |
| ! | TCCAACCCAATTTTCGATAT+CGG | + | chr4.3:19428182-19428201 | None:intergenic | 35.0% |
| ! | TGCATCTGAGATTAGTGTAA+TGG | - | chr4.3:19428625-19428644 | MS.gene28722:CDS | 35.0% |
| ! | TTTAAGGTGGGTGAATATGA+AGG | + | chr4.3:19427213-19427232 | None:intergenic | 35.0% |
| ! | TTTTGGTATAACTATCCGGT+AGG | - | chr4.3:19428077-19428096 | MS.gene28722:CDS | 35.0% |
| !! | AAATTGGGTTGGATGGTATA+TGG | - | chr4.3:19428189-19428208 | MS.gene28722:CDS | 35.0% |
| !! | AAGTAGGTAACTTCTGAGAT+TGG | + | chr4.3:19427180-19427199 | None:intergenic | 35.0% |
| !! | ACATTGGAGAAGGGTATTAT+TGG | + | chr4.3:19427130-19427149 | None:intergenic | 35.0% |
| !! | ACTTCTGAGATTGGATTAGA+AGG | + | chr4.3:19427171-19427190 | None:intergenic | 35.0% |
| !! | ATATCGAAAATTGGGTTGGA+TGG | - | chr4.3:19428182-19428201 | MS.gene28722:CDS | 35.0% |
| !!! | AATGGGGGAAGATGTTTTTT+GGG | - | chr4.3:19427701-19427720 | MS.gene28722:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | - | chr4.3:19427711-19427730 | MS.gene28722:CDS | 35.0% |
| !!! | GGATTGATTCAGTGTTGTTT+TGG | - | chr4.3:19428060-19428079 | MS.gene28722:CDS | 35.0% |
| AAAACATTGGAACAAGGTGG+TGG | - | chr4.3:19427822-19427841 | MS.gene28722:CDS | 40.0% | |
| AACATGTTACCAGCTCTATG+TGG | + | chr4.3:19428305-19428324 | None:intergenic | 40.0% | |
| AAGACACTGGTACTAGAATG+TGG | + | chr4.3:19427071-19427090 | None:intergenic | 40.0% | |
| ACAGAATCTGAAGAAGACAC+TGG | + | chr4.3:19427084-19427103 | None:intergenic | 40.0% | |
| ACATGTTACCAGCTCTATGT+GGG | + | chr4.3:19428304-19428323 | None:intergenic | 40.0% | |
| AGAGAGAAAGACATTGGAGA+AGG | + | chr4.3:19427140-19427159 | None:intergenic | 40.0% | |
| AGATGGGGTGAGAATGAAAA+CGG | + | chr4.3:19427283-19427302 | None:intergenic | 40.0% | |
| ATATAGGTGTCAATGGAGAG+GGG | - | chr4.3:19428471-19428490 | MS.gene28722:CDS | 40.0% | |
| ATATCAGTCGAAGTTCCTAC+CGG | + | chr4.3:19428095-19428114 | None:intergenic | 40.0% | |
| CAGAAGCCGATATCGAAAAT+TGG | - | chr4.3:19428173-19428192 | MS.gene28722:CDS | 40.0% | |
| CATGTTCTTGCTTAGATCAG+TGG | - | chr4.3:19427431-19427450 | MS.gene28722:CDS | 40.0% | |
| CTAATCTCAGATGCAAGTGA+TGG | + | chr4.3:19428620-19428639 | None:intergenic | 40.0% | |
| CTCACATTCAATCCTTATGG+TGG | - | chr4.3:19428242-19428261 | MS.gene28722:CDS | 40.0% | |
| GAAATCAGAATCAGAAGTGG+TGG | - | chr4.3:19427354-19427373 | MS.gene28722:CDS | 40.0% | |
| GAGAGAAAGACATTGGAGAA+GGG | + | chr4.3:19427139-19427158 | None:intergenic | 40.0% | |
| GATATAGGTGTCAATGGAGA+GGG | - | chr4.3:19428470-19428489 | MS.gene28722:CDS | 40.0% | |
| GATGTGAGAGATATGAGATG+GGG | + | chr4.3:19427298-19427317 | None:intergenic | 40.0% | |
| GCTGAGATTAGTAGTGTTCA+TGG | - | chr4.3:19427525-19427544 | MS.gene28722:CDS | 40.0% | |
| GGATGTGAGAGATATGAGAT+GGG | + | chr4.3:19427299-19427318 | None:intergenic | 40.0% | |
| GTTAGTCCTGCTAAGAGAAA+GGG | - | chr4.3:19427912-19427931 | MS.gene28722:CDS | 40.0% | |
| TCACCAATCAAACCCAATTC+AGG | + | chr4.3:19428020-19428039 | None:intergenic | 40.0% | |
| TCTGATGTAGGAGTTTAAGG+TGG | + | chr4.3:19427226-19427245 | None:intergenic | 40.0% | |
| TCTTGATAGGGAATCAATGG+GGG | - | chr4.3:19427686-19427705 | MS.gene28722:CDS | 40.0% | |
| TGATATAGGTGTCAATGGAG+AGG | - | chr4.3:19428469-19428488 | MS.gene28722:CDS | 40.0% | |
| TGCAGCATATGATTGATGCT+TGG | + | chr4.3:19427320-19427339 | None:intergenic | 40.0% | |
| TGGATGTGAGAGATATGAGA+TGG | + | chr4.3:19427300-19427319 | None:intergenic | 40.0% | |
| TGTTAGTCCTGCTAAGAGAA+AGG | - | chr4.3:19427911-19427930 | MS.gene28722:CDS | 40.0% | |
| TTAGTCCTGCTAAGAGAAAG+GGG | - | chr4.3:19427913-19427932 | MS.gene28722:CDS | 40.0% | |
| ! | AATGAGTGAGATTTCTGAGG+TGG | - | chr4.3:19428268-19428287 | MS.gene28722:CDS | 40.0% |
| ! | CTGATGTAGGAGTTTAAGGT+GGG | + | chr4.3:19427225-19427244 | None:intergenic | 40.0% |
| ! | GAATCTTTGTGGGTTGAATC+AGG | - | chr4.3:19427471-19427490 | MS.gene28722:CDS | 40.0% |
| ! | GGTGAATATGAAGGTGTGTT+TGG | + | chr4.3:19427204-19427223 | None:intergenic | 40.0% |
| ! | GTTGAATCAGGTGCTACAAT+TGG | - | chr4.3:19427483-19427502 | MS.gene28722:CDS | 40.0% |
| ! | TCACTCATTTTCCCACCATA+AGG | + | chr4.3:19428257-19428276 | None:intergenic | 40.0% |
| !! | GTGAGAATGAAAACGGGTTT+TGG | + | chr4.3:19427276-19427295 | None:intergenic | 40.0% |
| !! | TTGGTGGACATTTTAGTGGT+GGG | - | chr4.3:19427580-19427599 | MS.gene28722:CDS | 40.0% |
| !!! | CAATGGGGGAAGATGTTTTT+TGG | - | chr4.3:19427700-19427719 | MS.gene28722:CDS | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+TGG | - | chr4.3:19427714-19427733 | MS.gene28722:CDS | 40.0% |
| AGTGGTGGTCATGACTATGA+TGG | - | chr4.3:19427369-19427388 | MS.gene28722:CDS | 45.0% | |
| GATGGGGTGAGAATGAAAAC+GGG | + | chr4.3:19427282-19427301 | None:intergenic | 45.0% | |
| GCCGATATCGAAAATTGGGT+TGG | - | chr4.3:19428178-19428197 | MS.gene28722:CDS | 45.0% | |
| GTTTGTCCTACTGTTGGTGT+TGG | - | chr4.3:19427561-19427580 | MS.gene28722:CDS | 45.0% | |
| TATAGGTGTCAATGGAGAGG+GGG | - | chr4.3:19428472-19428491 | MS.gene28722:CDS | 45.0% | |
| TTACCAGCTCTATGTGGGAA+TGG | + | chr4.3:19428299-19428318 | None:intergenic | 45.0% | |
| TTCTTCCCCTTTCTCTTAGC+AGG | + | chr4.3:19427921-19427940 | None:intergenic | 45.0% | |
| ! | AGTGTTCATGGTTTTCCAGC+TGG | - | chr4.3:19427537-19427556 | MS.gene28722:CDS | 45.0% |
| ! | GTGGTGATGTTGTTCATCAG+TGG | - | chr4.3:19427841-19427860 | MS.gene28722:CDS | 45.0% |
| ! | GTGTTCATGGTTTTCCAGCT+GGG | - | chr4.3:19427538-19427557 | MS.gene28722:CDS | 45.0% |
| ! | TGAAGGTGTGTTTGGGAAGT+AGG | + | chr4.3:19427196-19427215 | None:intergenic | 45.0% |
| ! | TGTTCATGGTTTTCCAGCTG+GGG | - | chr4.3:19427539-19427558 | MS.gene28722:CDS | 45.0% |
| ! | TTTTGGGCTATTAGAGGTGG+TGG | - | chr4.3:19427717-19427736 | MS.gene28722:CDS | 45.0% |
| !! | GTTGGTGGACATTTTAGTGG+TGG | - | chr4.3:19427579-19427598 | MS.gene28722:CDS | 45.0% |
| !! | TGGTGGACATTTTAGTGGTG+GGG | - | chr4.3:19427581-19427600 | MS.gene28722:CDS | 45.0% |
| !!! | GAAAACGGGTTTTGGAGTAG+TGG | + | chr4.3:19427268-19427287 | None:intergenic | 45.0% |
| !!! | GGTGTTGGTGGACATTTTAG+TGG | - | chr4.3:19427576-19427595 | MS.gene28722:CDS | 45.0% |
| ACACCATTCCCACATAGAGC+TGG | - | chr4.3:19428293-19428312 | MS.gene28722:CDS | 50.0% | |
| TGTCCACCAACACCAACAGT+AGG | + | chr4.3:19427570-19427589 | None:intergenic | 50.0% | |
| !! | CATTTTAGTGGTGGGGGGTA+TGG | - | chr4.3:19427588-19427607 | MS.gene28722:CDS | 50.0% |
| !! | GGTGGACATTTTAGTGGTGG+GGG | - | chr4.3:19427582-19427601 | MS.gene28722:CDS | 50.0% |
| !! | GTGGACATTTTAGTGGTGGG+GGG | - | chr4.3:19427583-19427602 | MS.gene28722:CDS | 50.0% |
| !! | TGTCCTACTGTTGGTGTTGG+TGG | - | chr4.3:19427564-19427583 | MS.gene28722:CDS | 50.0% |
| ACAGTAGGACAAACCCCAGC+TGG | + | chr4.3:19427555-19427574 | None:intergenic | 55.0% | |
| GCTGGGGTTTGTCCTACTGT+TGG | - | chr4.3:19427555-19427574 | MS.gene28722:CDS | 55.0% | |
| GTCAATGGAGAGGGGGATGT+TGG | - | chr4.3:19428479-19428498 | MS.gene28722:CDS | 55.0% | |
| ! | TGGGCTATTAGAGGTGGTGG+TGG | - | chr4.3:19427720-19427739 | MS.gene28722:CDS | 55.0% |
| !!! | GGTGGTGGTGGAGCTAGTTT+TGG | - | chr4.3:19427732-19427751 | MS.gene28722:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 19427024 | 19428655 | 19427024 | ID=MS.gene28722 |
| chr4.3 | mRNA | 19427024 | 19428655 | 19427024 | ID=MS.gene28722.t1;Parent=MS.gene28722 |
| chr4.3 | exon | 19427024 | 19428655 | 19427024 | ID=MS.gene28722.t1.exon1;Parent=MS.gene28722.t1 |
| chr4.3 | CDS | 19427024 | 19428655 | 19427024 | ID=cds.MS.gene28722.t1;Parent=MS.gene28722.t1 |
| Gene Sequence |
| Protein sequence |
