Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28772.t1 | RHN62754.1 | 96.4 | 224 | 8 | 0 | 1 | 224 | 1 | 224 | 4.50E-98 | 367.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28772.t1 | Q9M2C0 | 60.5 | 200 | 70 | 2 | 26 | 224 | 7 | 198 | 1.7e-61 | 237.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28772.t1 | A0A396IER6 | 96.4 | 224 | 8 | 0 | 1 | 224 | 1 | 224 | 3.3e-98 | 367.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051323 | MS.gene28772 | 0.807372 | 5.22E-50 | -1.69E-46 |
| MS.gene051325 | MS.gene28772 | 0.886236 | 4.08E-72 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28772 | MS.gene006180 | PPI |
| MS.gene67394 | MS.gene28772 | PPI |
| MS.gene28772 | MS.gene59206 | PPI |
| MS.gene28772 | MS.gene60673 | PPI |
| MS.gene28772 | MS.gene000219 | PPI |
| MS.gene28772 | MS.gene67394 | PPI |
| MS.gene28772 | MS.gene066222 | PPI |
| MS.gene000219 | MS.gene28772 | PPI |
| MS.gene48173 | MS.gene28772 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28772.t1 | MTR_4g094332 | 96.429 | 224 | 8 | 0 | 1 | 224 | 1 | 224 | 1.25e-138 | 387 |
| MS.gene28772.t1 | MTR_4g094328 | 94.643 | 224 | 12 | 0 | 1 | 224 | 1 | 224 | 1.93e-134 | 377 |
| MS.gene28772.t1 | MTR_4g094330 | 94.643 | 224 | 12 | 0 | 1 | 224 | 1 | 224 | 1.93e-134 | 377 |
| MS.gene28772.t1 | MTR_1g099010 | 66.169 | 201 | 64 | 1 | 24 | 224 | 21 | 217 | 4.43e-91 | 267 |
| MS.gene28772.t1 | MTR_6g034975 | 66.029 | 209 | 68 | 2 | 16 | 224 | 5 | 210 | 1.94e-87 | 257 |
| MS.gene28772.t1 | MTR_4g094325 | 61.321 | 212 | 78 | 2 | 13 | 224 | 32 | 239 | 2.81e-84 | 250 |
| MS.gene28772.t1 | MTR_0119s0010 | 61.053 | 190 | 65 | 1 | 30 | 219 | 27 | 207 | 7.68e-73 | 220 |
| MS.gene28772.t1 | MTR_0119s0020 | 61.053 | 190 | 65 | 1 | 30 | 219 | 27 | 207 | 7.68e-73 | 220 |
| MS.gene28772.t1 | MTR_2g008110 | 61.053 | 190 | 65 | 1 | 30 | 219 | 27 | 207 | 8.95e-73 | 220 |
| MS.gene28772.t1 | MTR_4g094335 | 57.561 | 205 | 78 | 2 | 18 | 219 | 27 | 225 | 2.06e-70 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28772.t1 | AT3G43660 | 62.000 | 200 | 67 | 2 | 26 | 224 | 7 | 198 | 5.08e-91 | 266 |
| MS.gene28772.t1 | AT1G21140 | 61.809 | 199 | 67 | 2 | 26 | 224 | 11 | 200 | 3.10e-81 | 241 |
| MS.gene28772.t1 | AT3G43630 | 61.307 | 199 | 68 | 2 | 24 | 221 | 4 | 194 | 2.61e-80 | 239 |
| MS.gene28772.t1 | AT1G76800 | 61.500 | 200 | 68 | 1 | 25 | 224 | 6 | 196 | 2.82e-79 | 236 |
| MS.gene28772.t1 | AT3G25190 | 62.559 | 211 | 73 | 2 | 18 | 224 | 9 | 217 | 7.69e-74 | 223 |
Find 52 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGCTATGGCCATTACTTT+TGG | 0.296297 | 4.3:-19941089 | MS.gene28772:CDS |
| ACCAAATTGATTGGCACTAC+TGG | 0.296615 | 4.3:-19941062 | MS.gene28772:CDS |
| TCTTGTGTTAGAGTTCTTAT+TGG | 0.297338 | 4.3:-19941119 | MS.gene28772:CDS |
| TGTGGTTAGCTTTGCTTTGT+TGG | 0.303507 | 4.3:-19941189 | MS.gene28772:CDS |
| TTCAACTTTGAGAGTACTAT+TGG | 0.313072 | 4.3:+19941634 | None:intergenic |
| TCCAGTAGTGCCAATCAATT+TGG | 0.330163 | 4.3:+19941061 | None:intergenic |
| TTTGGTTTCACCAAATTGAT+TGG | 0.341339 | 4.3:-19941071 | MS.gene28772:CDS |
| AGCTTTGCTTTGTTGGTGTT+CGG | 0.375879 | 4.3:-19941182 | MS.gene28772:CDS |
| CTGCATTTATAAAGAATCAT+AGG | 0.383563 | 4.3:-19941235 | MS.gene28772:CDS |
| ATCTCAACCTCAAAATTGTT+TGG | 0.384795 | 4.3:+19941668 | None:intergenic |
| ATCAAAGTAATGCTCCTTGC+TGG | 0.393708 | 4.3:-19941485 | MS.gene28772:CDS |
| AATGCTGCTTGAAATGGATT+TGG | 0.394757 | 4.3:+19941308 | None:intergenic |
| TCCTTCATGGCCTCACAACT+TGG | 0.407619 | 4.3:-19941713 | None:intergenic |
| GAAGCTAATGCTGCTTGAAA+TGG | 0.414506 | 4.3:+19941302 | None:intergenic |
| ATGCTCCTTGCTGGTTTCGC+TGG | 0.425505 | 4.3:-19941476 | MS.gene28772:CDS |
| TGGCTTAGAGCAGCAATGTT+AGG | 0.431906 | 4.3:-19941572 | MS.gene28772:CDS |
| TATTGGAGGTTGGATGGCTA+TGG | 0.439649 | 4.3:-19941102 | MS.gene28772:CDS |
| TGTGTTAGAGTTCTTATTGG+AGG | 0.446628 | 4.3:-19941116 | MS.gene28772:CDS |
| TTAGAGTTCTTATTGGAGGT+TGG | 0.455085 | 4.3:-19941112 | MS.gene28772:CDS |
| AAAGAATCATAGGATTAGAA+TGG | 0.457336 | 4.3:-19941225 | MS.gene28772:CDS |
| AACAGTGATTAGTCCATCAT+TGG | 0.469248 | 4.3:+19941547 | None:intergenic |
| GGTTTCGCTGGTTTAGTAGC+CGG | 0.470478 | 4.3:-19941464 | MS.gene28772:CDS |
| CAGCATTGATTACTTTCAAA+GGG | 0.487104 | 4.3:-19941600 | MS.gene28772:CDS |
| ACAGCATTGATTACTTTCAA+AGG | 0.499048 | 4.3:-19941601 | MS.gene28772:CDS |
| AAGAATCATAGGATTAGAAT+GGG | 0.503124 | 4.3:-19941224 | MS.gene28772:CDS |
| GGAGGAGTGGGTGCATTGGT+TGG | 0.503269 | 4.3:-19941161 | MS.gene28772:CDS |
| AGAGTACTATTGGCATGGAT+AGG | 0.503952 | 4.3:+19941644 | None:intergenic |
| AGTTCTTATTGGAGGTTGGA+TGG | 0.512108 | 4.3:-19941108 | MS.gene28772:CDS |
| GTTCGGAGGAGTGGGTGCAT+TGG | 0.514286 | 4.3:-19941165 | MS.gene28772:CDS |
| GCCTCATTGATGATCGGCGT+TGG | 0.515998 | 4.3:-19941524 | MS.gene28772:CDS |
| AATGGGAGTTGTTGCTGCTG+TGG | 0.525549 | 4.3:-19941207 | MS.gene28772:CDS |
| GAGTACTATTGGCATGGATA+GGG | 0.527712 | 4.3:+19941645 | None:intergenic |
| AACCTCAAAATTGTTTGGTG+AGG | 0.527938 | 4.3:+19941673 | None:intergenic |
| CTTTGAGAGTACTATTGGCA+TGG | 0.540482 | 4.3:+19941639 | None:intergenic |
| TTGTTGGTGTTCGGAGGAGT+GGG | 0.540620 | 4.3:-19941173 | MS.gene28772:CDS |
| TTTGGTGAAACCAAAAGTAA+TGG | 0.542621 | 4.3:+19941079 | None:intergenic |
| AGCAGCATTAGCTTCCGCAC+TGG | 0.542923 | 4.3:-19941294 | MS.gene28772:CDS |
| ATAAATGCAGCTGCTAGTAA+TGG | 0.554872 | 4.3:+19941248 | None:intergenic |
| TTTGTTGGTGTTCGGAGGAG+TGG | 0.555153 | 4.3:-19941174 | MS.gene28772:CDS |
| GGTGCTTGTAGCATGGCAAT+TGG | 0.557712 | 4.3:-19941443 | MS.gene28772:CDS |
| AATTGCCATGCTACAAGCAC+CGG | 0.567135 | 4.3:+19941445 | None:intergenic |
| AGTAGCCGGTGCTTGTAGCA+TGG | 0.572629 | 4.3:-19941450 | MS.gene28772:CDS |
| TTTGCTTTGTTGGTGTTCGG+AGG | 0.573531 | 4.3:-19941179 | MS.gene28772:CDS |
| GCAATGTTAGGAGCCAATGA+TGG | 0.586770 | 4.3:-19941560 | MS.gene28772:CDS |
| ACTGTTGCCTCATTGATGAT+CGG | 0.595345 | 4.3:-19941530 | MS.gene28772:CDS |
| CTAACACAAGACCTCATCAA+AGG | 0.604425 | 4.3:+19941131 | None:intergenic |
| TGATGATGAAAGTAGTGAAA+GGG | 0.611622 | 4.3:-19941339 | MS.gene28772:CDS |
| ATTACTTTCAAAGGGCACAA+TGG | 0.615521 | 4.3:-19941592 | MS.gene28772:CDS |
| ATGATGATGAAAGTAGTGAA+AGG | 0.636055 | 4.3:-19941340 | MS.gene28772:CDS |
| CTAAACCAGCGAAACCAGCA+AGG | 0.672858 | 4.3:+19941471 | None:intergenic |
| TCCAACGCCGATCATCAATG+AGG | 0.683388 | 4.3:+19941523 | None:intergenic |
| TCCAATAGAAAATGCCAGTG+CGG | 0.690209 | 4.3:+19941280 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAATCATAGGATTAGAA+TGG | - | chr4.3:19941537-19941556 | MS.gene28772:CDS | 25.0% |
| ! | AAGAATCATAGGATTAGAAT+GGG | - | chr4.3:19941538-19941557 | MS.gene28772:CDS | 25.0% |
| ! | CTGCATTTATAAAGAATCAT+AGG | - | chr4.3:19941527-19941546 | MS.gene28772:CDS | 25.0% |
| ATCTCAACCTCAAAATTGTT+TGG | + | chr4.3:19941097-19941116 | None:intergenic | 30.0% | |
| TCTTGTGTTAGAGTTCTTAT+TGG | - | chr4.3:19941643-19941662 | MS.gene28772:CDS | 30.0% | |
| TTTGGTGAAACCAAAAGTAA+TGG | + | chr4.3:19941686-19941705 | None:intergenic | 30.0% | |
| ! | ATGATGATGAAAGTAGTGAA+AGG | - | chr4.3:19941422-19941441 | MS.gene28772:CDS | 30.0% |
| ! | TGATGATGAAAGTAGTGAAA+GGG | - | chr4.3:19941423-19941442 | MS.gene28772:CDS | 30.0% |
| ! | TTTGGTTTCACCAAATTGAT+TGG | - | chr4.3:19941691-19941710 | MS.gene28772:CDS | 30.0% |
| !! | ACAGCATTGATTACTTTCAA+AGG | - | chr4.3:19941161-19941180 | MS.gene28772:CDS | 30.0% |
| !! | CAGCATTGATTACTTTCAAA+GGG | - | chr4.3:19941162-19941181 | MS.gene28772:CDS | 30.0% |
| !! | TTCAACTTTGAGAGTACTAT+TGG | + | chr4.3:19941131-19941150 | None:intergenic | 30.0% |
| ATAAATGCAGCTGCTAGTAA+TGG | + | chr4.3:19941517-19941536 | None:intergenic | 35.0% | |
| CAACTTGGAGCAAAAACTAA+TGG | - | chr4.3:19941064-19941083 | MS.gene28772:CDS | 35.0% | |
| TGTGTTAGAGTTCTTATTGG+AGG | - | chr4.3:19941646-19941665 | MS.gene28772:CDS | 35.0% | |
| TTAGAGTTCTTATTGGAGGT+TGG | - | chr4.3:19941650-19941669 | MS.gene28772:CDS | 35.0% | |
| TTGGAAAAACTCCTTTGATG+AGG | - | chr4.3:19941620-19941639 | MS.gene28772:CDS | 35.0% | |
| ! | AACAGTGATTAGTCCATCAT+TGG | + | chr4.3:19941218-19941237 | None:intergenic | 35.0% |
| ! | AACCTCAAAATTGTTTGGTG+AGG | + | chr4.3:19941092-19941111 | None:intergenic | 35.0% |
| ! | AACCTCACCAAACAATTTTG+AGG | - | chr4.3:19941087-19941106 | MS.gene28772:CDS | 35.0% |
| ! | AATGCTGCTTGAAATGGATT+TGG | + | chr4.3:19941457-19941476 | None:intergenic | 35.0% |
| ! | ATTACTTTCAAAGGGCACAA+TGG | - | chr4.3:19941170-19941189 | MS.gene28772:CDS | 35.0% |
| ACCAAATTGATTGGCACTAC+TGG | - | chr4.3:19941700-19941719 | MS.gene28772:CDS | 40.0% | |
| AGTTCTTATTGGAGGTTGGA+TGG | - | chr4.3:19941654-19941673 | MS.gene28772:CDS | 40.0% | |
| ATCAAAGTAATGCTCCTTGC+TGG | - | chr4.3:19941277-19941296 | MS.gene28772:CDS | 40.0% | |
| CTAACACAAGACCTCATCAA+AGG | + | chr4.3:19941634-19941653 | None:intergenic | 40.0% | |
| GAGTACTATTGGCATGGATA+GGG | + | chr4.3:19941120-19941139 | None:intergenic | 40.0% | |
| TCCAATAGAAAATGCCAGTG+CGG | + | chr4.3:19941485-19941504 | None:intergenic | 40.0% | |
| TCCAGTAGTGCCAATCAATT+TGG | + | chr4.3:19941704-19941723 | None:intergenic | 40.0% | |
| ! | ACTGTTGCCTCATTGATGAT+CGG | - | chr4.3:19941232-19941251 | MS.gene28772:CDS | 40.0% |
| ! | AGAGTACTATTGGCATGGAT+AGG | + | chr4.3:19941121-19941140 | None:intergenic | 40.0% |
| ! | AGCTTTGCTTTGTTGGTGTT+CGG | - | chr4.3:19941580-19941599 | MS.gene28772:CDS | 40.0% |
| ! | AGTTTTTGCTCCAAGTTGTG+AGG | + | chr4.3:19941062-19941081 | None:intergenic | 40.0% |
| ! | ATGGCTATGGCCATTACTTT+TGG | - | chr4.3:19941673-19941692 | MS.gene28772:CDS | 40.0% |
| ! | GAAGCTAATGCTGCTTGAAA+TGG | + | chr4.3:19941463-19941482 | None:intergenic | 40.0% |
| !! | CTTTGAGAGTACTATTGGCA+TGG | + | chr4.3:19941126-19941145 | None:intergenic | 40.0% |
| !! | TGTGGTTAGCTTTGCTTTGT+TGG | - | chr4.3:19941573-19941592 | MS.gene28772:CDS | 40.0% |
| AATTGCCATGCTACAAGCAC+CGG | + | chr4.3:19941320-19941339 | None:intergenic | 45.0% | |
| GCAATGTTAGGAGCCAATGA+TGG | - | chr4.3:19941202-19941221 | MS.gene28772:CDS | 45.0% | |
| TATTGGAGGTTGGATGGCTA+TGG | - | chr4.3:19941660-19941679 | MS.gene28772:CDS | 45.0% | |
| TGGCTTAGAGCAGCAATGTT+AGG | - | chr4.3:19941190-19941209 | MS.gene28772:CDS | 45.0% | |
| ! | TTTGCTTTGTTGGTGTTCGG+AGG | - | chr4.3:19941583-19941602 | MS.gene28772:CDS | 45.0% |
| !! | TCCGCACTGGCATTTTCTAT+TGG | - | chr4.3:19941481-19941500 | MS.gene28772:CDS | 45.0% |
| AATGGGAGTTGTTGCTGCTG+TGG | - | chr4.3:19941555-19941574 | MS.gene28772:CDS | 50.0% | |
| CTAAACCAGCGAAACCAGCA+AGG | + | chr4.3:19941294-19941313 | None:intergenic | 50.0% | |
| GGTGCTTGTAGCATGGCAAT+TGG | - | chr4.3:19941319-19941338 | MS.gene28772:CDS | 50.0% | |
| TCCAACGCCGATCATCAATG+AGG | + | chr4.3:19941242-19941261 | None:intergenic | 50.0% | |
| ! | TTGTTGGTGTTCGGAGGAGT+GGG | - | chr4.3:19941589-19941608 | MS.gene28772:CDS | 50.0% |
| ! | TTTGTTGGTGTTCGGAGGAG+TGG | - | chr4.3:19941588-19941607 | MS.gene28772:CDS | 50.0% |
| !! | GGTTTCGCTGGTTTAGTAGC+CGG | - | chr4.3:19941298-19941317 | MS.gene28772:CDS | 50.0% |
| AGTAGCCGGTGCTTGTAGCA+TGG | - | chr4.3:19941312-19941331 | MS.gene28772:CDS | 55.0% | |
| ! | AGCAGCATTAGCTTCCGCAC+TGG | - | chr4.3:19941468-19941487 | MS.gene28772:CDS | 55.0% |
| ! | GCCTCATTGATGATCGGCGT+TGG | - | chr4.3:19941238-19941257 | MS.gene28772:CDS | 55.0% |
| !! | ATGCTCCTTGCTGGTTTCGC+TGG | - | chr4.3:19941286-19941305 | MS.gene28772:CDS | 55.0% |
| GTTCGGAGGAGTGGGTGCAT+TGG | - | chr4.3:19941597-19941616 | MS.gene28772:CDS | 60.0% | |
| ! | GGAGGAGTGGGTGCATTGGT+TGG | - | chr4.3:19941601-19941620 | MS.gene28772:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 19941055 | 19941729 | 19941055 | ID=MS.gene28772 |
| chr4.3 | mRNA | 19941055 | 19941729 | 19941055 | ID=MS.gene28772.t1;Parent=MS.gene28772 |
| chr4.3 | exon | 19941055 | 19941729 | 19941055 | ID=MS.gene28772.t1.exon1;Parent=MS.gene28772.t1 |
| chr4.3 | CDS | 19941055 | 19941729 | 19941055 | ID=cds.MS.gene28772.t1;Parent=MS.gene28772.t1 |
| Gene Sequence |
| Protein sequence |
