AlfalfaGEDB | MS.gene59206

M. sativa cultivar XinJiangDaYe / MS.gene59206

KEGG: K22736
GO: GO:0003674 GO:0005215 GO:0005384 GO:0006873 GO:0006875 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015318 GO:0019725 GO:0022857 GO:0022890 GO:0030003 GO:0030026 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771
PFAM: PF01988.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59206.t1	XP_003592125.1	94.1	222	7	2	1	221	1	217	6.20E-100	373.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59206.t1	Q9M2C0	67.0	206	56	2	19	221	2	198	8.3e-69	261.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59206.t1	G7ID02	94.1	222	7	2	1	221	1	217	4.5e-100	373.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene59206	0.828768	7.48E-55	-1.69E-46
MS.gene049669	MS.gene59206	0.813034	3.14E-51	-1.69E-46
MS.gene050168	MS.gene59206	0.803288	3.74E-49	-1.69E-46
MS.gene050226	MS.gene59206	0.804548	2.05E-49	-1.69E-46
MS.gene050442	MS.gene59206	0.833254	5.93E-56	-1.69E-46
MS.gene050680	MS.gene59206	0.857709	1.36E-62	-1.69E-46
MS.gene050750	MS.gene59206	0.814391	1.58E-51	-1.69E-46
MS.gene050953	MS.gene59206	0.818838	1.59E-52	-1.69E-46
MS.gene051368	MS.gene59206	0.84166	4.15E-58	-1.69E-46
MS.gene051535	MS.gene59206	0.824774	6.73E-54	-1.69E-46
MS.gene051983	MS.gene59206	0.821566	3.77E-53	-1.69E-46
MS.gene052176	MS.gene59206	0.805203	1.50E-49	-1.69E-46
MS.gene052333	MS.gene59206	0.819678	1.02E-52	-1.69E-46
MS.gene052426	MS.gene59206	0.848264	6.85E-60	-1.69E-46
MS.gene053166	MS.gene59206	0.833657	4.70E-56	-1.69E-46
MS.gene053216	MS.gene59206	0.806655	7.40E-50	-1.69E-46
MS.gene053494	MS.gene59206	0.838311	3.10E-57	-1.69E-46
MS.gene054405	MS.gene59206	0.816032	6.81E-52	-1.69E-46
MS.gene054559	MS.gene59206	0.822536	2.25E-53	-1.69E-46
MS.gene054930	MS.gene59206	0.821734	3.45E-53	-1.69E-46
MS.gene05510	MS.gene59206	0.867129	1.72E-65	-1.69E-46
MS.gene055230	MS.gene59206	0.802941	4.42E-49	-1.69E-46
MS.gene055367	MS.gene59206	0.804385	2.22E-49	-1.69E-46
MS.gene055623	MS.gene59206	0.809083	2.26E-50	-1.69E-46
MS.gene055701	MS.gene59206	0.88802	8.54E-73	-1.69E-46
MS.gene056480	MS.gene59206	0.819079	1.40E-52	-1.69E-46
MS.gene056506	MS.gene59206	0.81518	1.05E-51	-1.69E-46
MS.gene056979	MS.gene59206	0.81087	9.30E-51	-1.69E-46
MS.gene057156	MS.gene59206	-0.818475	1.92E-52	-1.69E-46
MS.gene057394	MS.gene59206	0.800548	1.37E-48	-1.69E-46
MS.gene05761	MS.gene59206	0.821828	3.28E-53	-1.69E-46
MS.gene058146	MS.gene59206	0.829349	5.41E-55	-1.69E-46
MS.gene058435	MS.gene59206	0.835677	1.46E-56	-1.69E-46
MS.gene058595	MS.gene59206	0.813052	3.11E-51	-1.69E-46
MS.gene059128	MS.gene59206	0.817069	3.99E-52	-1.69E-46
MS.gene059161	MS.gene59206	0.820906	5.36E-53	-1.69E-46
MS.gene059609	MS.gene59206	0.805862	1.09E-49	-1.69E-46
MS.gene060011	MS.gene59206	0.814486	1.50E-51	-1.69E-46
MS.gene060322	MS.gene59206	0.810455	1.14E-50	-1.69E-46
MS.gene060683	MS.gene59206	0.830366	3.06E-55	-1.69E-46
MS.gene060685	MS.gene59206	0.827362	1.63E-54	-1.69E-46
MS.gene060998	MS.gene59206	0.823302	1.49E-53	-1.69E-46
MS.gene061114	MS.gene59206	-0.802093	6.61E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene59206	MS.gene35050	PPI
MS.gene006180	MS.gene59206	PPI
MS.gene35053	MS.gene59206	PPI
MS.gene028404	MS.gene59206	PPI
MS.gene28772	MS.gene59206	PPI
MS.gene066222	MS.gene59206	PPI
MS.gene028406	MS.gene59206	PPI
MS.gene59206	MS.gene28770	PPI
MS.gene35051	MS.gene59206	PPI
MS.gene60673	MS.gene59206	PPI
MS.gene48173	MS.gene59206	PPI
MS.gene28770	MS.gene59206	PPI
MS.gene59206	MS.gene028405	PPI
MS.gene59206	MS.gene70400	PPI
MS.gene051323	MS.gene59206	PPI
MS.gene000219	MS.gene59206	PPI
MS.gene70401	MS.gene59206	PPI
MS.gene028403	MS.gene59206	PPI
MS.gene59206	MS.gene28771	PPI
MS.gene67394	MS.gene59206	PPI
MS.gene051325	MS.gene59206	PPI
MS.gene35052	MS.gene59206	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59206.t1	MTR_1g099010	96.847	222	1	2	1	221	1	217	9.29e-153	422
MS.gene59206.t1	MTR_6g034975	79.630	216	37	3	6	221	2	210	3.66e-107	307
MS.gene59206.t1	MTR_4g094328	64.019	214	76	1	8	221	12	224	9.84e-86	253
MS.gene59206.t1	MTR_4g094330	64.019	214	76	1	8	221	12	224	9.84e-86	253
MS.gene59206.t1	MTR_4g094332	66.832	202	66	1	20	221	24	224	1.66e-85	253
MS.gene59206.t1	MTR_4g094325	61.611	211	73	2	15	221	33	239	8.25e-84	249
MS.gene59206.t1	MTR_0119s0010	68.783	189	49	1	28	216	29	207	8.20e-80	238
MS.gene59206.t1	MTR_0119s0020	68.783	189	49	1	28	216	29	207	8.20e-80	238
MS.gene59206.t1	MTR_2g008110	68.783	189	49	1	28	216	29	207	9.45e-80	238
MS.gene59206.t1	MTR_4g094335	57.870	216	84	3	9	221	20	231	6.07e-71	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59206.t1	AT3G43660	68.447	206	53	2	19	221	2	198	1.29e-100	290
MS.gene59206.t1	AT3G43630	73.039	204	41	4	18	218	2	194	5.25e-94	273
MS.gene59206.t1	AT1G21140	70.673	208	48	4	18	221	2	200	5.61e-94	273
MS.gene59206.t1	AT1G76800	71.707	205	48	1	17	221	2	196	1.38e-92	270
MS.gene59206.t1	AT3G25190	59.434	212	81	2	14	221	7	217	1.29e-67	207

Find 40 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTAAGGGTAGGTGTTGTTT+TGG	0.263367	1.3:-68102265	MS.gene59206:CDS
ATCTTTCATTAAAGATTATA+AGG	0.280155	1.3:-68102286	MS.gene59206:CDS
ATTGCTATGGCCATAACTTT+TGG	0.287767	1.3:-68102141	MS.gene59206:CDS
GGTTGGTTGGGTGCAGTGTT+GGG	0.338405	1.3:-68102213	MS.gene59206:CDS
TTTGGACTAACCAAGTTGAT+TGG	0.369529	1.3:-68102123	MS.gene59206:CDS
TGGTTGGTTGGGTGCAGTGT+TGG	0.376731	1.3:-68102214	MS.gene59206:CDS
TTTGGTTGTGTTTGGTTGGT+TGG	0.383193	1.3:-68102226	MS.gene59206:CDS
GCTGCTGCTTGTAAAGGATT+TGG	0.384499	1.3:+68102360	None:intergenic
CTTGGTTAGTCCAAAAGTTA+TGG	0.395661	1.3:+68102131	None:intergenic
TTGGTTGTGTTTGGTTGGTT+GGG	0.399852	1.3:-68102225	MS.gene59206:CDS
CAGTGGTAAGGTCTTGTTTG+AGG	0.403097	1.3:-68102182	MS.gene59206:CDS
AGTGGTAAGGTCTTGTTTGA+GGG	0.418892	1.3:-68102181	MS.gene59206:CDS
TTCAACAGCATCACTAATGA+TGG	0.422094	1.3:-68102586	MS.gene59206:CDS
GTTGGGTGCAGTGTTGGGTA+AGG	0.428457	1.3:-68102208	MS.gene59206:CDS
ATAAAAGCTATGATCTTGTC+AGG	0.430620	1.3:-68102540	MS.gene59206:CDS
TCTTGTTTGAGGGTCTTACT+TGG	0.431071	1.3:-68102171	MS.gene59206:CDS
TGTTTGAGGGTCTTACTTGG+AGG	0.451549	1.3:-68102168	MS.gene59206:CDS
ACCAAGTTGATTGGGTCAAG+TGG	0.451627	1.3:-68102114	MS.gene59206:CDS
TTGGACTAACCAAGTTGATT+GGG	0.452121	1.3:-68102122	MS.gene59206:CDS
TTGCTTTGGTTGTGTTTGGT+TGG	0.457210	1.3:-68102230	MS.gene59206:CDS
ACTTGGAGGTTGGATTGCTA+TGG	0.458097	1.3:-68102154	MS.gene59206:CDS
TGAGGGTCTTACTTGGAGGT+TGG	0.468585	1.3:-68102164	MS.gene59206:CDS
CCCACTTGACCCAATCAACT+TGG	0.477807	1.3:+68102113	None:intergenic
TCATTAAAGATTATAAGGTA+AGG	0.478111	1.3:-68102281	MS.gene59206:CDS
AAAAGAGACAATATTGAAAG+AGG	0.486922	1.3:-68102426	MS.gene59206:CDS
CATTAAAGATTATAAGGTAA+GGG	0.537509	1.3:-68102280	MS.gene59206:CDS
GGTGCATGTAGCATGGCAAT+AGG	0.539596	1.3:-68102498	MS.gene59206:CDS
GAAGCTGCTGCTGCTTGTAA+AGG	0.540279	1.3:+68102354	None:intergenic
ATGAAAGATGCTGCAAGAAG+TGG	0.543716	1.3:+68102300	None:intergenic
TGGGTAAGGCACCAGTGGTA+AGG	0.548709	1.3:-68102194	MS.gene59206:CDS
AAAGATTATAAGGTAAGGGT+AGG	0.576224	1.3:-68102276	MS.gene59206:CDS
CCAAGTTGATTGGGTCAAGT+GGG	0.582206	1.3:-68102113	MS.gene59206:CDS
ATCACAATGGCTACGTGCCG+CGG	0.590039	1.3:-68102634	MS.gene59206:CDS
GCATCACTAATGATGGGTGT+AGG	0.595742	1.3:-68102579	MS.gene59206:CDS
AAGAGGTAATAATATTGAAG+AGG	0.603524	1.3:-68102409	MS.gene59206:CDS
CTCAAACAAGACCTTACCAC+TGG	0.603707	1.3:+68102183	None:intergenic
TCAACAGCATCACTAATGAT+GGG	0.621364	1.3:-68102585	MS.gene59206:CDS
ATCGTTAGCTCCTAAAACCG+CGG	0.633149	1.3:+68102617	None:intergenic
AGTGTTGGGTAAGGCACCAG+TGG	0.645814	1.3:-68102199	MS.gene59206:CDS
TGTAGCTGGTGCATGTAGCA+TGG	0.650697	1.3:-68102505	MS.gene59206:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTTTCATTAAAGATTATA+AGG	-	chr1.3:68102571-68102590	MS.gene59206:CDS	15.0%
!!	CATTAAAGATTATAAGGTAA+GGG	-	chr1.3:68102577-68102596	MS.gene59206:CDS	20.0%
!!	TCATTAAAGATTATAAGGTA+AGG	-	chr1.3:68102576-68102595	MS.gene59206:CDS	20.0%
!!!	TACAAATTTTGTTTGGTTAA+GGG	+	chr1.3:68102129-68102148	None:intergenic	20.0%
!!!	TTTGATGTACAAATTTTGTT+TGG	+	chr1.3:68102136-68102155	None:intergenic	20.0%
!	AAAAGAGACAATATTGAAAG+AGG	-	chr1.3:68102431-68102450	MS.gene59206:CDS	25.0%
!	AAGAGGTAATAATATTGAAG+AGG	-	chr1.3:68102448-68102467	MS.gene59206:CDS	25.0%
!	ATTACTCAAAAAGATCACAA+TGG	-	chr1.3:68102210-68102229	MS.gene59206:CDS	25.0%
!!!	GTACAAATTTTGTTTGGTTA+AGG	+	chr1.3:68102130-68102149	None:intergenic	25.0%
	AAAGATTATAAGGTAAGGGT+AGG	-	chr1.3:68102581-68102600	MS.gene59206:CDS	30.0%
	ATAAAAGCTATGATCTTGTC+AGG	-	chr1.3:68102317-68102336	MS.gene59206:CDS	30.0%
!!	TCAGCTTTAGCATTTTCAAT+TGG	-	chr1.3:68102524-68102543	MS.gene59206:CDS	30.0%
	TCAACAGCATCACTAATGAT+GGG	-	chr1.3:68102272-68102291	MS.gene59206:CDS	35.0%
	TTCAACAGCATCACTAATGA+TGG	-	chr1.3:68102271-68102290	MS.gene59206:CDS	35.0%
!	AGCATTTTCAATTGGTGCAA+TGG	-	chr1.3:68102532-68102551	MS.gene59206:CDS	35.0%
!	AGTTTTGCTTTGGTTGTGTT+TGG	-	chr1.3:68102623-68102642	MS.gene59206:CDS	35.0%
!	ATTGCTATGGCCATAACTTT+TGG	-	chr1.3:68102716-68102735	MS.gene59206:CDS	35.0%
!	CTTGGTTAGTCCAAAAGTTA+TGG	+	chr1.3:68102729-68102748	None:intergenic	35.0%
!	GTGTTGCATTTTGTTGATCA+TGG	+	chr1.3:68102166-68102185	None:intergenic	35.0%
!!	TTGGACTAACCAAGTTGATT+GGG	-	chr1.3:68102735-68102754	MS.gene59206:CDS	35.0%
!!	TTTGGACTAACCAAGTTGAT+TGG	-	chr1.3:68102734-68102753	MS.gene59206:CDS	35.0%
	ATGAAAGATGCTGCAAGAAG+TGG	+	chr1.3:68102560-68102579	None:intergenic	40.0%
	TTGCTTTGGTTGTGTTTGGT+TGG	-	chr1.3:68102627-68102646	MS.gene59206:CDS	40.0%
	TTTGGTTGTGTTTGGTTGGT+TGG	-	chr1.3:68102631-68102650	MS.gene59206:CDS	40.0%
!	ATGATCTTGTCAGGTTTTGC+TGG	-	chr1.3:68102326-68102345	MS.gene59206:CDS	40.0%
!	TCTTGTTTGAGGGTCTTACT+TGG	-	chr1.3:68102686-68102705	MS.gene59206:CDS	40.0%
!	TTGGTTGTGTTTGGTTGGTT+GGG	-	chr1.3:68102632-68102651	MS.gene59206:CDS	40.0%
!!	AGTGGTAAGGTCTTGTTTGA+GGG	-	chr1.3:68102676-68102695	MS.gene59206:CDS	40.0%
!!!	GTAAGGGTAGGTGTTGTTTT+GGG	-	chr1.3:68102593-68102612	MS.gene59206:CDS	40.0%
	ACTTGGAGGTTGGATTGCTA+TGG	-	chr1.3:68102703-68102722	MS.gene59206:CDS	45.0%
	ATCGTTAGCTCCTAAAACCG+CGG	+	chr1.3:68102243-68102262	None:intergenic	45.0%
	CTCAAACAAGACCTTACCAC+TGG	+	chr1.3:68102677-68102696	None:intergenic	45.0%
	TGTTTGAGGGTCTTACTTGG+AGG	-	chr1.3:68102689-68102708	MS.gene59206:CDS	45.0%
!	GCTGCTGCTTGTAAAGGATT+TGG	+	chr1.3:68102500-68102519	None:intergenic	45.0%
!!	ACCAAGTTGATTGGGTCAAG+TGG	-	chr1.3:68102743-68102762	MS.gene59206:CDS	45.0%
!!	CAGTGGTAAGGTCTTGTTTG+AGG	-	chr1.3:68102675-68102694	MS.gene59206:CDS	45.0%
!!	CCAAGTTGATTGGGTCAAGT+GGG	-	chr1.3:68102744-68102763	MS.gene59206:CDS	45.0%
!!	GCATCACTAATGATGGGTGT+AGG	-	chr1.3:68102278-68102297	MS.gene59206:CDS	45.0%
!!!	GGGAGCAGTTAGTTTTGCTT+TGG	-	chr1.3:68102613-68102632	MS.gene59206:CDS	45.0%
!!!	GGTAAGGGTAGGTGTTGTTT+TGG	-	chr1.3:68102592-68102611	MS.gene59206:CDS	45.0%
	CCCACTTGACCCAATCAACT+TGG	+	chr1.3:68102747-68102766	None:intergenic	50.0%
	GGTGCATGTAGCATGGCAAT+AGG	-	chr1.3:68102359-68102378	MS.gene59206:CDS	50.0%
	TGAGGGTCTTACTTGGAGGT+TGG	-	chr1.3:68102693-68102712	MS.gene59206:CDS	50.0%
	TGTAGCTGGTGCATGTAGCA+TGG	-	chr1.3:68102352-68102371	MS.gene59206:CDS	50.0%
!	GAAGCTGCTGCTGCTTGTAA+AGG	+	chr1.3:68102506-68102525	None:intergenic	50.0%
!	GGTTTTGCTGGACTTGTAGC+TGG	-	chr1.3:68102338-68102357	MS.gene59206:CDS	50.0%
!!!	GCGGTTTTAGGAGCTAACGA+TGG	-	chr1.3:68102242-68102261	MS.gene59206:CDS	50.0%
	ATCACAATGGCTACGTGCCG+CGG	-	chr1.3:68102223-68102242	MS.gene59206:CDS	55.0%
!	AGTGTTGGGTAAGGCACCAG+TGG	-	chr1.3:68102658-68102677	MS.gene59206:CDS	55.0%
!	GGTTGGTTGGGTGCAGTGTT+GGG	-	chr1.3:68102644-68102663	MS.gene59206:CDS	55.0%
!	GTTGGGTGCAGTGTTGGGTA+AGG	-	chr1.3:68102649-68102668	MS.gene59206:CDS	55.0%
!	TGGGTAAGGCACCAGTGGTA+AGG	-	chr1.3:68102663-68102682	MS.gene59206:CDS	55.0%
!	TGGTTGGTTGGGTGCAGTGT+TGG	-	chr1.3:68102643-68102662	MS.gene59206:CDS	55.0%
!	TGGCTACGTGCCGCGGTTTT+AGG	-	chr1.3:68102230-68102249	MS.gene59206:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	68102107	68102772	68102107	ID=MS.gene59206
chr1.3	mRNA	68102107	68102772	68102107	ID=MS.gene59206.t1;Parent=MS.gene59206
chr1.3	exon	68102107	68102772	68102107	ID=MS.gene59206.t1.exon1;Parent=MS.gene59206.t1
chr1.3	CDS	68102107	68102772	68102107	ID=cds.MS.gene59206.t1;Parent=MS.gene59206.t1

Gene Sequence

>MS.gene59206

ATGGCATCTAATAATTCATCCCTTAACCAAACAAAATTTGTACATCAAAACAATGACCATGATCAACAAAATGCAACACTAGAGATCGAAAGTGATGAATTTGATTACTCAAAAAGATCACAATGGCTACGTGCCGCGGTTTTAGGAGCTAACGATGGATTAGTTTCAACAGCATCACTAATGATGGGTGTAGGAGCAGTGAAACAAGACATAAAAGCTATGATCTTGTCAGGTTTTGCTGGACTTGTAGCTGGTGCATGTAGCATGGCAATAGGTGAGTTTGTATCTGTTTATTCACAACTTGACATTGAAATTGCTCAATTAAAAAGAGACAATATTGAAAGAGGTAATAATATTGAAGAGGAAAATGTTATTGAGAAAGAAAGTTTGCCAAATCCTTTACAAGCAGCAGCAGCTTCAGCTTTAGCATTTTCAATTGGTGCAATGGTGCCACTTCTTGCAGCATCTTTCATTAAAGATTATAAGGTAAGGGTAGGTGTTGTTTTGGGAGCAGTTAGTTTTGCTTTGGTTGTGTTTGGTTGGTTGGGTGCAGTGTTGGGTAAGGCACCAGTGGTAAGGTCTTGTTTGAGGGTCTTACTTGGAGGTTGGATTGCTATGGCCATAACTTTTGGACTAACCAAGTTGATTGGGTCAAGTGGGCTATAG

Protein sequence

>MS.gene59206.t1

MASNNSSLNQTKFVHQNNDHDQQNATLEIESDEFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILSGFAGLVAGACSMAIGEFVSVYSQLDIEIAQLKRDNIERGNNIEEENVIEKESLPNPLQAAAASALAFSIGAMVPLLAASFIKDYKVRVGVVLGAVSFALVVFGWLGAVLGKAPVVRSCLRVLLGGWIAMAITFGLTKLIGSSGL