Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066222.t1 | XP_003592125.1 | 96.4 | 222 | 2 | 2 | 1 | 221 | 1 | 217 | 1.60E-103 | 385.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066222.t1 | Q9LPU9 | 68.4 | 209 | 56 | 2 | 14 | 221 | 1 | 200 | 1.5e-70 | 267.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066222.t1 | G7ID02 | 96.4 | 222 | 2 | 2 | 1 | 221 | 1 | 217 | 1.1e-103 | 385.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051535 | MS.gene066222 | 0.806727 | 7.15E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene066222 | 0.817432 | 3.31E-52 | -1.69E-46 |
| MS.gene053494 | MS.gene066222 | 0.801834 | 7.47E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene066222 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene066222 | 0.837836 | 4.11E-57 | -1.69E-46 |
| MS.gene056979 | MS.gene066222 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene058435 | MS.gene066222 | 0.82601 | 3.43E-54 | -1.69E-46 |
| MS.gene059161 | MS.gene066222 | 0.801362 | 9.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60673 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene028405 | PPI |
| MS.gene48173 | MS.gene066222 | PPI |
| MS.gene70401 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene59206 | PPI |
| MS.gene066222 | MS.gene70400 | PPI |
| MS.gene67394 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene60673 | PPI |
| MS.gene051325 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene28771 | PPI |
| MS.gene051323 | MS.gene066222 | PPI |
| MS.gene000219 | MS.gene066222 | PPI |
| MS.gene028403 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene35050 | PPI |
| MS.gene35053 | MS.gene066222 | PPI |
| MS.gene35052 | MS.gene066222 | PPI |
| MS.gene028404 | MS.gene066222 | PPI |
| MS.gene28772 | MS.gene066222 | PPI |
| MS.gene028406 | MS.gene066222 | PPI |
| MS.gene006180 | MS.gene066222 | PPI |
| MS.gene35051 | MS.gene066222 | PPI |
| MS.gene066222 | MS.gene28770 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066222.t1 | MTR_1g099010 | 96.396 | 222 | 2 | 2 | 1 | 221 | 1 | 217 | 1.61e-152 | 422 |
| MS.gene066222.t1 | MTR_6g034975 | 79.167 | 216 | 38 | 3 | 6 | 221 | 2 | 210 | 3.43e-107 | 307 |
| MS.gene066222.t1 | MTR_4g094328 | 64.019 | 214 | 76 | 1 | 8 | 221 | 12 | 224 | 1.04e-85 | 253 |
| MS.gene066222.t1 | MTR_4g094330 | 64.019 | 214 | 76 | 1 | 8 | 221 | 12 | 224 | 1.04e-85 | 253 |
| MS.gene066222.t1 | MTR_4g094332 | 66.832 | 202 | 66 | 1 | 20 | 221 | 24 | 224 | 1.98e-85 | 253 |
| MS.gene066222.t1 | MTR_4g094325 | 62.085 | 211 | 72 | 2 | 15 | 221 | 33 | 239 | 4.15e-84 | 249 |
| MS.gene066222.t1 | MTR_0119s0010 | 68.783 | 189 | 49 | 2 | 28 | 216 | 29 | 207 | 5.55e-79 | 236 |
| MS.gene066222.t1 | MTR_0119s0020 | 68.783 | 189 | 49 | 2 | 28 | 216 | 29 | 207 | 5.55e-79 | 236 |
| MS.gene066222.t1 | MTR_2g008110 | 68.783 | 189 | 49 | 2 | 28 | 216 | 29 | 207 | 6.19e-79 | 236 |
| MS.gene066222.t1 | MTR_4g094335 | 58.333 | 216 | 83 | 3 | 9 | 221 | 20 | 231 | 6.77e-71 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066222.t1 | AT3G43660 | 68.447 | 206 | 53 | 3 | 19 | 221 | 2 | 198 | 2.38e-100 | 290 |
| MS.gene066222.t1 | AT3G43630 | 72.549 | 204 | 42 | 4 | 18 | 218 | 2 | 194 | 7.78e-94 | 273 |
| MS.gene066222.t1 | AT1G21140 | 70.192 | 208 | 49 | 4 | 18 | 221 | 2 | 200 | 1.14e-93 | 273 |
| MS.gene066222.t1 | AT1G76800 | 71.707 | 205 | 48 | 1 | 17 | 221 | 2 | 196 | 1.16e-92 | 270 |
| MS.gene066222.t1 | AT3G25190 | 59.434 | 212 | 81 | 2 | 14 | 221 | 7 | 217 | 2.02e-68 | 209 |
Find 43 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAAGGGTAGGTGTTGTTT+TGG | 0.269938 | 1.1:-71191877 | MS.gene066222:CDS |
| ATTGCTATGGCCATAACTTT+TGG | 0.287767 | 1.1:-71191753 | MS.gene066222:CDS |
| GGTTGGTTGGGTGCAGTGTT+GGG | 0.338405 | 1.1:-71191825 | MS.gene066222:CDS |
| TTTGGACTAACCAAGTTGAT+TGG | 0.369529 | 1.1:-71191735 | MS.gene066222:CDS |
| TGGTTGGTTGGGTGCAGTGT+TGG | 0.376731 | 1.1:-71191826 | MS.gene066222:CDS |
| TTTGGTTGTGTTTGGTTGGT+TGG | 0.383193 | 1.1:-71191838 | MS.gene066222:CDS |
| GCTGCTGCTTGTAAAGGATT+TGG | 0.384499 | 1.1:+71191972 | None:intergenic |
| ATTCATCACTTTCGATCTCT+AGG | 0.393578 | 1.1:+71192285 | None:intergenic |
| CTTGGTTAGTCCAAAAGTTA+TGG | 0.395661 | 1.1:+71191743 | None:intergenic |
| CATTAAAGATTATAAGATAA+GGG | 0.398036 | 1.1:-71191892 | MS.gene066222:CDS |
| TTGGTTGTGTTTGGTTGGTT+GGG | 0.399852 | 1.1:-71191837 | MS.gene066222:CDS |
| CAGTGGTAAGGTCTTGTTTG+AGG | 0.403097 | 1.1:-71191794 | MS.gene066222:CDS |
| AGTGGTAAGGTCTTGTTTGA+GGG | 0.418892 | 1.1:-71191793 | MS.gene066222:CDS |
| TTCAACAGCATCACTAATGA+TGG | 0.422094 | 1.1:-71192198 | MS.gene066222:CDS |
| GTTGGGTGCAGTGTTGGGTA+AGG | 0.428457 | 1.1:-71191820 | MS.gene066222:CDS |
| TCTTGTTTGAGGGTCTTGCT+TGG | 0.436159 | 1.1:-71191783 | MS.gene066222:CDS |
| ACCAAGTTGATTGGGTCAAG+TGG | 0.451627 | 1.1:-71191726 | MS.gene066222:CDS |
| TTGGACTAACCAAGTTGATT+GGG | 0.452121 | 1.1:-71191734 | MS.gene066222:CDS |
| TGTTTGAGGGTCTTGCTTGG+AGG | 0.456354 | 1.1:-71191780 | MS.gene066222:CDS |
| TTGCTTTGGTTGTGTTTGGT+TGG | 0.457210 | 1.1:-71191842 | MS.gene066222:CDS |
| TCATTAAAGATTATAAGATA+AGG | 0.464621 | 1.1:-71191893 | MS.gene066222:CDS |
| GCTTGGAGGTTGGATTGCTA+TGG | 0.470569 | 1.1:-71191766 | MS.gene066222:CDS |
| CCCACTTGACCCAATCAACT+TGG | 0.477807 | 1.1:+71191725 | None:intergenic |
| AAAAGAGACAATATTGAAAG+AGG | 0.486922 | 1.1:-71192038 | MS.gene066222:CDS |
| ATAAAAGCCATGATCTTGTC+GGG | 0.488331 | 1.1:-71192152 | MS.gene066222:CDS |
| TGAGGGTCTTGCTTGGAGGT+TGG | 0.503434 | 1.1:-71191776 | MS.gene066222:CDS |
| CATAAAAGCCATGATCTTGT+CGG | 0.507773 | 1.1:-71192153 | MS.gene066222:CDS |
| TTCATCACTTTCGATCTCTA+GGG | 0.515825 | 1.1:+71192286 | None:intergenic |
| AGCAAAACCCGACAAGATCA+TGG | 0.526585 | 1.1:+71192145 | None:intergenic |
| GGTGCATGTAGCATGGCAAT+AGG | 0.539596 | 1.1:-71192110 | MS.gene066222:CDS |
| GAAGCTGCTGCTGCTTGTAA+AGG | 0.540279 | 1.1:+71191966 | None:intergenic |
| ATGAAAGATGCTGCAAGAAG+TGG | 0.543716 | 1.1:+71191912 | None:intergenic |
| TGGGTAAGGCACCAGTGGTA+AGG | 0.548709 | 1.1:-71191806 | MS.gene066222:CDS |
| AAGAGGTAATAATATTGAAG+AGG | 0.573020 | 1.1:-71192021 | MS.gene066222:CDS |
| AAAGATTATAAGATAAGGGT+AGG | 0.576224 | 1.1:-71191888 | MS.gene066222:CDS |
| CCAAGTTGATTGGGTCAAGT+GGG | 0.582206 | 1.1:-71191725 | MS.gene066222:CDS |
| ATCACAATGGCTACGTGCCG+CGG | 0.590039 | 1.1:-71192246 | MS.gene066222:CDS |
| GCATCACTAATGATGGGTGT+AGG | 0.595742 | 1.1:-71192191 | MS.gene066222:CDS |
| CTCAAACAAGACCTTACCAC+TGG | 0.603707 | 1.1:+71191795 | None:intergenic |
| ATCATTAGCTCCTAAAACCG+CGG | 0.609497 | 1.1:+71192229 | None:intergenic |
| TCAACAGCATCACTAATGAT+GGG | 0.621364 | 1.1:-71192197 | MS.gene066222:CDS |
| AGTGTTGGGTAAGGCACCAG+TGG | 0.645814 | 1.1:-71191811 | MS.gene066222:CDS |
| TGTAGCTGGTGCATGTAGCA+TGG | 0.650697 | 1.1:-71192117 | MS.gene066222:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTAAAGATTATAAGATAA+GGG | - | chr1.1:71192189-71192208 | MS.gene066222:CDS | 15.0% |
| !! | TCATTAAAGATTATAAGATA+AGG | - | chr1.1:71192188-71192207 | MS.gene066222:CDS | 15.0% |
| !!! | TACAAATTTTGTTTGGTTAA+GGG | + | chr1.1:71191741-71191760 | None:intergenic | 20.0% |
| !!! | TTTGATGTACAAATTTTGTT+TGG | + | chr1.1:71191748-71191767 | None:intergenic | 20.0% |
| ! | AAAAGAGACAATATTGAAAG+AGG | - | chr1.1:71192043-71192062 | MS.gene066222:CDS | 25.0% |
| ! | AAAGATTATAAGATAAGGGT+AGG | - | chr1.1:71192193-71192212 | MS.gene066222:CDS | 25.0% |
| ! | AAGAGGTAATAATATTGAAG+AGG | - | chr1.1:71192060-71192079 | MS.gene066222:CDS | 25.0% |
| ! | ATTACTCAAAAAGATCACAA+TGG | - | chr1.1:71191822-71191841 | MS.gene066222:CDS | 25.0% |
| !!! | GTACAAATTTTGTTTGGTTA+AGG | + | chr1.1:71191742-71191761 | None:intergenic | 25.0% |
| !! | TCAGCTTTAGCATTTTCAAT+TGG | - | chr1.1:71192136-71192155 | MS.gene066222:CDS | 30.0% |
| ATAAAAGCCATGATCTTGTC+GGG | - | chr1.1:71191929-71191948 | MS.gene066222:CDS | 35.0% | |
| ATTCATCACTTTCGATCTCT+AGG | + | chr1.1:71191799-71191818 | None:intergenic | 35.0% | |
| CATAAAAGCCATGATCTTGT+CGG | - | chr1.1:71191928-71191947 | MS.gene066222:CDS | 35.0% | |
| TCAACAGCATCACTAATGAT+GGG | - | chr1.1:71191884-71191903 | MS.gene066222:CDS | 35.0% | |
| TTCAACAGCATCACTAATGA+TGG | - | chr1.1:71191883-71191902 | MS.gene066222:CDS | 35.0% | |
| TTCATCACTTTCGATCTCTA+GGG | + | chr1.1:71191798-71191817 | None:intergenic | 35.0% | |
| ! | AGCATTTTCAATTGGTGCAA+TGG | - | chr1.1:71192144-71192163 | MS.gene066222:CDS | 35.0% |
| ! | AGTTTTGCTTTGGTTGTGTT+TGG | - | chr1.1:71192235-71192254 | MS.gene066222:CDS | 35.0% |
| ! | ATTGCTATGGCCATAACTTT+TGG | - | chr1.1:71192328-71192347 | MS.gene066222:CDS | 35.0% |
| ! | CTTGGTTAGTCCAAAAGTTA+TGG | + | chr1.1:71192341-71192360 | None:intergenic | 35.0% |
| !! | TTGGACTAACCAAGTTGATT+GGG | - | chr1.1:71192347-71192366 | MS.gene066222:CDS | 35.0% |
| !! | TTTGGACTAACCAAGTTGAT+TGG | - | chr1.1:71192346-71192365 | MS.gene066222:CDS | 35.0% |
| !!! | ATAAGGGTAGGTGTTGTTTT+GGG | - | chr1.1:71192205-71192224 | MS.gene066222:CDS | 35.0% |
| ATCATTAGCTCCTAAAACCG+CGG | + | chr1.1:71191855-71191874 | None:intergenic | 40.0% | |
| ATGAAAGATGCTGCAAGAAG+TGG | + | chr1.1:71192172-71192191 | None:intergenic | 40.0% | |
| TTGCTTTGGTTGTGTTTGGT+TGG | - | chr1.1:71192239-71192258 | MS.gene066222:CDS | 40.0% | |
| TTTGGTTGTGTTTGGTTGGT+TGG | - | chr1.1:71192243-71192262 | MS.gene066222:CDS | 40.0% | |
| ! | GGGTTGCATTTTGTTGATCA+TGG | + | chr1.1:71191778-71191797 | None:intergenic | 40.0% |
| ! | TTGGTTGTGTTTGGTTGGTT+GGG | - | chr1.1:71192244-71192263 | MS.gene066222:CDS | 40.0% |
| !! | AGTGGTAAGGTCTTGTTTGA+GGG | - | chr1.1:71192288-71192307 | MS.gene066222:CDS | 40.0% |
| !!! | GATAAGGGTAGGTGTTGTTT+TGG | - | chr1.1:71192204-71192223 | MS.gene066222:CDS | 40.0% |
| AGCAAAACCCGACAAGATCA+TGG | + | chr1.1:71191939-71191958 | None:intergenic | 45.0% | |
| CTCAAACAAGACCTTACCAC+TGG | + | chr1.1:71192289-71192308 | None:intergenic | 45.0% | |
| ! | ATGATCTTGTCGGGTTTTGC+TGG | - | chr1.1:71191938-71191957 | MS.gene066222:CDS | 45.0% |
| ! | GCTGCTGCTTGTAAAGGATT+TGG | + | chr1.1:71192112-71192131 | None:intergenic | 45.0% |
| ! | TCTTGTTTGAGGGTCTTGCT+TGG | - | chr1.1:71192298-71192317 | MS.gene066222:CDS | 45.0% |
| !! | ACCAAGTTGATTGGGTCAAG+TGG | - | chr1.1:71192355-71192374 | MS.gene066222:CDS | 45.0% |
| !! | CAGTGGTAAGGTCTTGTTTG+AGG | - | chr1.1:71192287-71192306 | MS.gene066222:CDS | 45.0% |
| !! | CCAAGTTGATTGGGTCAAGT+GGG | - | chr1.1:71192356-71192375 | MS.gene066222:CDS | 45.0% |
| !! | GCATCACTAATGATGGGTGT+AGG | - | chr1.1:71191890-71191909 | MS.gene066222:CDS | 45.0% |
| !!! | GCGGTTTTAGGAGCTAATGA+TGG | - | chr1.1:71191854-71191873 | MS.gene066222:CDS | 45.0% |
| !!! | GGGAGCAGTTAGTTTTGCTT+TGG | - | chr1.1:71192225-71192244 | MS.gene066222:CDS | 45.0% |
| CCCACTTGACCCAATCAACT+TGG | + | chr1.1:71192359-71192378 | None:intergenic | 50.0% | |
| GCTTGGAGGTTGGATTGCTA+TGG | - | chr1.1:71192315-71192334 | MS.gene066222:CDS | 50.0% | |
| GGTGCATGTAGCATGGCAAT+AGG | - | chr1.1:71191971-71191990 | MS.gene066222:CDS | 50.0% | |
| TGTAGCTGGTGCATGTAGCA+TGG | - | chr1.1:71191964-71191983 | MS.gene066222:CDS | 50.0% | |
| TGTTTGAGGGTCTTGCTTGG+AGG | - | chr1.1:71192301-71192320 | MS.gene066222:CDS | 50.0% | |
| ! | GAAGCTGCTGCTGCTTGTAA+AGG | + | chr1.1:71192118-71192137 | None:intergenic | 50.0% |
| ! | GGTTTTGCTGGACTTGTAGC+TGG | - | chr1.1:71191950-71191969 | MS.gene066222:CDS | 50.0% |
| ATCACAATGGCTACGTGCCG+CGG | - | chr1.1:71191835-71191854 | MS.gene066222:CDS | 55.0% | |
| TGAGGGTCTTGCTTGGAGGT+TGG | - | chr1.1:71192305-71192324 | MS.gene066222:CDS | 55.0% | |
| ! | AGTGTTGGGTAAGGCACCAG+TGG | - | chr1.1:71192270-71192289 | MS.gene066222:CDS | 55.0% |
| ! | GGTTGGTTGGGTGCAGTGTT+GGG | - | chr1.1:71192256-71192275 | MS.gene066222:CDS | 55.0% |
| ! | GTTGGGTGCAGTGTTGGGTA+AGG | - | chr1.1:71192261-71192280 | MS.gene066222:CDS | 55.0% |
| ! | TGGGTAAGGCACCAGTGGTA+AGG | - | chr1.1:71192275-71192294 | MS.gene066222:CDS | 55.0% |
| ! | TGGTTGGTTGGGTGCAGTGT+TGG | - | chr1.1:71192255-71192274 | MS.gene066222:CDS | 55.0% |
| ! | TGGCTACGTGCCGCGGTTTT+AGG | - | chr1.1:71191842-71191861 | MS.gene066222:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 71191719 | 71192384 | 71191719 | ID=MS.gene066222 |
| chr1.1 | mRNA | 71191719 | 71192384 | 71191719 | ID=MS.gene066222.t1;Parent=MS.gene066222 |
| chr1.1 | exon | 71191719 | 71192384 | 71191719 | ID=MS.gene066222.t1.exon1;Parent=MS.gene066222.t1 |
| chr1.1 | CDS | 71191719 | 71192384 | 71191719 | ID=cds.MS.gene066222.t1;Parent=MS.gene066222.t1 |
| Gene Sequence |
| Protein sequence |
