AlfalfaGEDB | MS.gene051325

M. sativa cultivar XinJiangDaYe / MS.gene051325

KEGG: K22736
GO: GO:0003674 GO:0005215 GO:0005384 GO:0006873 GO:0006875 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015318 GO:0019725 GO:0022857 GO:0022890 GO:0030003 GO:0030026 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771
PFAM: PF01988.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051325.t1	RHN62754.1	94.6	224	12	0	1	224	1	224	6.10E-95	357.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051325.t1	Q9M2C0	59.0	200	73	2	26	224	7	198	6.0e-59	228.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051325.t1	A0A396IER6	94.6	224	12	0	1	224	1	224	4.4e-95	357.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051325	MS.gene28772	0.886236	4.08E-72	-1.69E-46
MS.gene051325	MS.gene35051	0.872635	2.73E-67	-1.69E-46
MS.gene051325	MS.gene70401	0.918058	2.49E-86	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene051325	MS.gene60673	PPI
MS.gene48173	MS.gene051325	PPI
MS.gene000219	MS.gene051325	PPI
MS.gene051325	MS.gene066222	PPI
MS.gene67394	MS.gene051325	PPI
MS.gene006180	MS.gene051325	PPI
MS.gene051325	MS.gene59206	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051325.t1	MTR_4g094332	95.982	224	9	0	1	224	1	224	4.60e-138	386
MS.gene051325.t1	MTR_4g094328	94.196	224	13	0	1	224	1	224	7.53e-134	375
MS.gene051325.t1	MTR_4g094330	94.196	224	13	0	1	224	1	224	7.53e-134	375
MS.gene051325.t1	MTR_1g099010	66.667	201	63	1	24	224	21	217	1.33e-91	268
MS.gene051325.t1	MTR_6g034975	66.986	209	66	2	16	224	5	210	8.67e-89	261
MS.gene051325.t1	MTR_4g094325	61.792	212	77	2	13	224	32	239	1.07e-84	251
MS.gene051325.t1	MTR_2g008110	61.053	190	65	1	30	219	27	207	6.11e-73	221
MS.gene051325.t1	MTR_0119s0010	61.053	190	65	1	30	219	27	207	6.60e-73	220
MS.gene051325.t1	MTR_0119s0020	61.053	190	65	1	30	219	27	207	6.60e-73	220
MS.gene051325.t1	MTR_4g094335	57.073	205	79	2	18	219	27	225	5.73e-70	214

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051325.t1	AT3G43660	63.000	200	65	2	26	224	7	198	5.39e-92	268
MS.gene051325.t1	AT1G21140	62.312	199	66	2	26	224	11	200	4.37e-82	243
MS.gene051325.t1	AT3G43630	61.809	199	67	2	24	221	4	194	5.52e-81	241
MS.gene051325.t1	AT1G76800	62.000	200	67	1	25	224	6	196	4.65e-80	238
MS.gene051325.t1	AT3G25190	63.033	211	72	2	18	224	9	217	5.31e-74	223

Find 51 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGCTATGGCCATTACTTT+TGG	0.296297	4.1:-19290674	MS.gene051325:CDS
ACCAAATTGATTGGCACTAC+TGG	0.296615	4.1:-19290647	MS.gene051325:CDS
TCTTGTGTTAGAGTTCTTAT+TGG	0.297338	4.1:-19290704	MS.gene051325:CDS
TGTGGTTAGCTTTGCTTTGT+TGG	0.303507	4.1:-19290774	MS.gene051325:CDS
TTCAACTTTGAGAGTACTAT+TGG	0.313072	4.1:+19291219	None:intergenic
TCCAGTAGTGCCAATCAATT+TGG	0.330163	4.1:+19290646	None:intergenic
TTTGGTTTCACCAAATTGAT+TGG	0.341339	4.1:-19290656	MS.gene051325:CDS
AGCTTTGCTTTGTTGGTGTT+CGG	0.375879	4.1:-19290767	MS.gene051325:CDS
CTGCATTTATAAAGAATCAT+AGG	0.383563	4.1:-19290820	MS.gene051325:CDS
ATCTCAACCTCAAAATTGTT+TGG	0.384795	4.1:+19291253	None:intergenic
ATCAAAGTAATGCTCCTTGC+TGG	0.393708	4.1:-19291070	MS.gene051325:CDS
AATGCTGCTTGAAATGGATT+TGG	0.394757	4.1:+19290893	None:intergenic
TCCTTCATGGCCTCACAACT+TGG	0.407619	4.1:-19291298	None:intergenic
GAAGCTAATGCTGCTTGAAA+TGG	0.414506	4.1:+19290887	None:intergenic
ATGCTCCTTGCTGGTTTCGC+TGG	0.425505	4.1:-19291061	MS.gene051325:CDS
TGGCTTAGAGCAGCAATGTT+AGG	0.431906	4.1:-19291157	MS.gene051325:CDS
TATTGGAGGTTGGATGGCTA+TGG	0.439649	4.1:-19290687	MS.gene051325:CDS
TGTGTTAGAGTTCTTATTGG+AGG	0.446628	4.1:-19290701	MS.gene051325:CDS
TTAGAGTTCTTATTGGAGGT+TGG	0.455085	4.1:-19290697	MS.gene051325:CDS
AAAGAATCATAGGATTAGAA+TGG	0.457336	4.1:-19290810	MS.gene051325:CDS
AACAGTGATTAGTCCATCAT+TGG	0.469248	4.1:+19291132	None:intergenic
GGTTTCGCTGGTTTAGTAGC+CGG	0.470478	4.1:-19291049	MS.gene051325:CDS
CAGCATTGATTACTTTCAAA+GGG	0.487104	4.1:-19291185	MS.gene051325:CDS
TGATGATGAAAGTACTGAAA+AGG	0.488855	4.1:-19290924	MS.gene051325:CDS
ACAGCATTGATTACTTTCAA+AGG	0.499048	4.1:-19291186	MS.gene051325:CDS
AAGAATCATAGGATTAGAAT+GGG	0.503124	4.1:-19290809	MS.gene051325:CDS
GGAGGAGTGGGTGCATTGGT+TGG	0.503269	4.1:-19290746	MS.gene051325:CDS
AGAGTACTATTGGCATGGAT+AGG	0.503952	4.1:+19291229	None:intergenic
AGTTCTTATTGGAGGTTGGA+TGG	0.512108	4.1:-19290693	MS.gene051325:CDS
GTTCGGAGGAGTGGGTGCAT+TGG	0.514286	4.1:-19290750	MS.gene051325:CDS
GCCTCATTGATGATCGGCGT+TGG	0.515998	4.1:-19291109	MS.gene051325:CDS
AATGGGAGTTGTTGCTGCTG+TGG	0.525549	4.1:-19290792	MS.gene051325:CDS
GAGTACTATTGGCATGGATA+GGG	0.527712	4.1:+19291230	None:intergenic
AACCTCAAAATTGTTTGGTG+AGG	0.527938	4.1:+19291258	None:intergenic
CTTTGAGAGTACTATTGGCA+TGG	0.540482	4.1:+19291224	None:intergenic
TTGTTGGTGTTCGGAGGAGT+GGG	0.540620	4.1:-19290758	MS.gene051325:CDS
TTTGGTGAAACCAAAAGTAA+TGG	0.542621	4.1:+19290664	None:intergenic
AGCAGCATTAGCTTCCGCAC+TGG	0.542923	4.1:-19290879	MS.gene051325:CDS
ATAAATGCAGCTGCTAGTAA+TGG	0.554872	4.1:+19290833	None:intergenic
TTTGTTGGTGTTCGGAGGAG+TGG	0.555153	4.1:-19290759	MS.gene051325:CDS
GGTGCTTGTAGCATGGCAAT+TGG	0.557712	4.1:-19291028	MS.gene051325:CDS
AATTGCCATGCTACAAGCAC+CGG	0.567135	4.1:+19291030	None:intergenic
AGTAGCCGGTGCTTGTAGCA+TGG	0.572629	4.1:-19291035	MS.gene051325:CDS
TTTGCTTTGTTGGTGTTCGG+AGG	0.573531	4.1:-19290764	MS.gene051325:CDS
GCAATGTTAGGAGCCAATGA+TGG	0.586770	4.1:-19291145	MS.gene051325:CDS
ACTGTTGCCTCATTGATGAT+CGG	0.595345	4.1:-19291115	MS.gene051325:CDS
CTAACACAAGACCTCATCAA+AGG	0.604425	4.1:+19290716	None:intergenic
ATTACTTTCAAAGGGCACAA+TGG	0.615521	4.1:-19291177	MS.gene051325:CDS
CTAAACCAGCGAAACCAGCA+AGG	0.672858	4.1:+19291056	None:intergenic
TCCAACGCCGATCATCAATG+AGG	0.683388	4.1:+19291108	None:intergenic
TCCAATAGAAAATGCCAGTG+CGG	0.690209	4.1:+19290865	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGAATCATAGGATTAGAA+TGG	-	chr4.1:19291122-19291141	MS.gene051325:CDS	25.0%
!	AAGAATCATAGGATTAGAAT+GGG	-	chr4.1:19291123-19291142	MS.gene051325:CDS	25.0%
!	CTGCATTTATAAAGAATCAT+AGG	-	chr4.1:19291112-19291131	MS.gene051325:CDS	25.0%
	ATCTCAACCTCAAAATTGTT+TGG	+	chr4.1:19290682-19290701	None:intergenic	30.0%
	TCTTGTGTTAGAGTTCTTAT+TGG	-	chr4.1:19291228-19291247	MS.gene051325:CDS	30.0%
	TTTGGTGAAACCAAAAGTAA+TGG	+	chr4.1:19291271-19291290	None:intergenic	30.0%
!	TTTGGTTTCACCAAATTGAT+TGG	-	chr4.1:19291276-19291295	MS.gene051325:CDS	30.0%
!!	ACAGCATTGATTACTTTCAA+AGG	-	chr4.1:19290746-19290765	MS.gene051325:CDS	30.0%
!!	CAGCATTGATTACTTTCAAA+GGG	-	chr4.1:19290747-19290766	MS.gene051325:CDS	30.0%
!!	TGATGATGAAAGTACTGAAA+AGG	-	chr4.1:19291008-19291027	MS.gene051325:CDS	30.0%
!!	TTCAACTTTGAGAGTACTAT+TGG	+	chr4.1:19290716-19290735	None:intergenic	30.0%
	ATAAATGCAGCTGCTAGTAA+TGG	+	chr4.1:19291102-19291121	None:intergenic	35.0%
	CAACTTGGAGCAAAAACTAA+TGG	-	chr4.1:19290649-19290668	MS.gene051325:CDS	35.0%
	TGTGTTAGAGTTCTTATTGG+AGG	-	chr4.1:19291231-19291250	MS.gene051325:CDS	35.0%
	TTAGAGTTCTTATTGGAGGT+TGG	-	chr4.1:19291235-19291254	MS.gene051325:CDS	35.0%
	TTGGAAAAACTCCTTTGATG+AGG	-	chr4.1:19291205-19291224	MS.gene051325:CDS	35.0%
!	AACAGTGATTAGTCCATCAT+TGG	+	chr4.1:19290803-19290822	None:intergenic	35.0%
!	AACCTCAAAATTGTTTGGTG+AGG	+	chr4.1:19290677-19290696	None:intergenic	35.0%
!	AACCTCACCAAACAATTTTG+AGG	-	chr4.1:19290672-19290691	MS.gene051325:CDS	35.0%
!	AATGCTGCTTGAAATGGATT+TGG	+	chr4.1:19291042-19291061	None:intergenic	35.0%
!	ATTACTTTCAAAGGGCACAA+TGG	-	chr4.1:19290755-19290774	MS.gene051325:CDS	35.0%
	ACCAAATTGATTGGCACTAC+TGG	-	chr4.1:19291285-19291304	MS.gene051325:CDS	40.0%
	AGTTCTTATTGGAGGTTGGA+TGG	-	chr4.1:19291239-19291258	MS.gene051325:CDS	40.0%
	ATCAAAGTAATGCTCCTTGC+TGG	-	chr4.1:19290862-19290881	MS.gene051325:CDS	40.0%
	CTAACACAAGACCTCATCAA+AGG	+	chr4.1:19291219-19291238	None:intergenic	40.0%
	GAGTACTATTGGCATGGATA+GGG	+	chr4.1:19290705-19290724	None:intergenic	40.0%
	TCCAATAGAAAATGCCAGTG+CGG	+	chr4.1:19291070-19291089	None:intergenic	40.0%
	TCCAGTAGTGCCAATCAATT+TGG	+	chr4.1:19291289-19291308	None:intergenic	40.0%
!	ACTGTTGCCTCATTGATGAT+CGG	-	chr4.1:19290817-19290836	MS.gene051325:CDS	40.0%
!	AGAGTACTATTGGCATGGAT+AGG	+	chr4.1:19290706-19290725	None:intergenic	40.0%
!	AGCTTTGCTTTGTTGGTGTT+CGG	-	chr4.1:19291165-19291184	MS.gene051325:CDS	40.0%
!	AGTTTTTGCTCCAAGTTGTG+AGG	+	chr4.1:19290647-19290666	None:intergenic	40.0%
!	ATGGCTATGGCCATTACTTT+TGG	-	chr4.1:19291258-19291277	MS.gene051325:CDS	40.0%
!	GAAGCTAATGCTGCTTGAAA+TGG	+	chr4.1:19291048-19291067	None:intergenic	40.0%
!!	CTTTGAGAGTACTATTGGCA+TGG	+	chr4.1:19290711-19290730	None:intergenic	40.0%
!!	TGTGGTTAGCTTTGCTTTGT+TGG	-	chr4.1:19291158-19291177	MS.gene051325:CDS	40.0%
	AATTGCCATGCTACAAGCAC+CGG	+	chr4.1:19290905-19290924	None:intergenic	45.0%
	GCAATGTTAGGAGCCAATGA+TGG	-	chr4.1:19290787-19290806	MS.gene051325:CDS	45.0%
	TATTGGAGGTTGGATGGCTA+TGG	-	chr4.1:19291245-19291264	MS.gene051325:CDS	45.0%
	TGGCTTAGAGCAGCAATGTT+AGG	-	chr4.1:19290775-19290794	MS.gene051325:CDS	45.0%
!	TTTGCTTTGTTGGTGTTCGG+AGG	-	chr4.1:19291168-19291187	MS.gene051325:CDS	45.0%
!!	TCCGCACTGGCATTTTCTAT+TGG	-	chr4.1:19291066-19291085	MS.gene051325:CDS	45.0%
	AATGGGAGTTGTTGCTGCTG+TGG	-	chr4.1:19291140-19291159	MS.gene051325:CDS	50.0%
	CTAAACCAGCGAAACCAGCA+AGG	+	chr4.1:19290879-19290898	None:intergenic	50.0%
	GGTGCTTGTAGCATGGCAAT+TGG	-	chr4.1:19290904-19290923	MS.gene051325:CDS	50.0%
	TCCAACGCCGATCATCAATG+AGG	+	chr4.1:19290827-19290846	None:intergenic	50.0%
!	TTGTTGGTGTTCGGAGGAGT+GGG	-	chr4.1:19291174-19291193	MS.gene051325:CDS	50.0%
!	TTTGTTGGTGTTCGGAGGAG+TGG	-	chr4.1:19291173-19291192	MS.gene051325:CDS	50.0%
!!	GGTTTCGCTGGTTTAGTAGC+CGG	-	chr4.1:19290883-19290902	MS.gene051325:CDS	50.0%
	AGTAGCCGGTGCTTGTAGCA+TGG	-	chr4.1:19290897-19290916	MS.gene051325:CDS	55.0%
!	AGCAGCATTAGCTTCCGCAC+TGG	-	chr4.1:19291053-19291072	MS.gene051325:CDS	55.0%
!	GCCTCATTGATGATCGGCGT+TGG	-	chr4.1:19290823-19290842	MS.gene051325:CDS	55.0%
!!	ATGCTCCTTGCTGGTTTCGC+TGG	-	chr4.1:19290871-19290890	MS.gene051325:CDS	55.0%
	GTTCGGAGGAGTGGGTGCAT+TGG	-	chr4.1:19291182-19291201	MS.gene051325:CDS	60.0%
!	GGAGGAGTGGGTGCATTGGT+TGG	-	chr4.1:19291186-19291205	MS.gene051325:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	19290640	19291314	19290640	ID=MS.gene051325
chr4.1	mRNA	19290640	19291314	19290640	ID=MS.gene051325.t1;Parent=MS.gene051325
chr4.1	exon	19290640	19291314	19290640	ID=MS.gene051325.t1.exon1;Parent=MS.gene051325.t1
chr4.1	CDS	19290640	19291314	19290640	ID=cds.MS.gene051325.t1;Parent=MS.gene051325.t1

Gene Sequence

>MS.gene051325

ATGGCCTCACAACTTGGAGCAAAAACTAATGGAACCTCACCAAACAATTTTGAGGTTGAGATCCCTATCCATGCCAATAGTACTCTCAAAGTTGAAAAAGAGAGCAACAGCATTGATTACTTTCAAAGGGCACAATGGCTTAGAGCAGCAATGTTAGGAGCCAATGATGGACTAATCACTGTTGCCTCATTGATGATCGGCGTTGGAGCTATTAAAGAAGACATCAAAGTAATGCTCCTTGCTGGTTTCGCTGGTTTAGTAGCCGGTGCTTGTAGCATGGCAATTGGTGAGTTTGTTTCTGTTTATACTCAATATGATATTGAGATAGCTCAGATAAAAAGAGAAAGAGAAGCTAATAATAATAACAATGATGATGAAAGTACTGAAAAGGAGAAATTGCCAAATCCATTTCAAGCAGCATTAGCTTCCGCACTGGCATTTTCTATTGGAGCTGTTATGCCATTACTAGCAGCTGCATTTATAAAGAATCATAGGATTAGAATGGGAGTTGTTGCTGCTGTGGTTAGCTTTGCTTTGTTGGTGTTCGGAGGAGTGGGTGCATTGGTTGGAAAAACTCCTTTGATGAGGTCTTGTGTTAGAGTTCTTATTGGAGGTTGGATGGCTATGGCCATTACTTTTGGTTTCACCAAATTGATTGGCACTACTGGACTTTGA

Protein sequence

>MS.gene051325.t1

MASQLGAKTNGTSPNNFEVEIPIHANSTLKVEKESNSIDYFQRAQWLRAAMLGANDGLITVASLMIGVGAIKEDIKVMLLAGFAGLVAGACSMAIGEFVSVYTQYDIEIAQIKREREANNNNNDDESTEKEKLPNPFQAALASALAFSIGAVMPLLAAAFIKNHRIRMGVVAAVVSFALLVFGGVGALVGKTPLMRSCVRVLIGGWMAMAITFGFTKLIGTTGL