Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28921.t1 | AES89816.1 | 77.3 | 344 | 77 | 1 | 1 | 343 | 163 | 506 | 6.20E-147 | 530.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28921.t1 | Q40392 | 37.2 | 339 | 199 | 7 | 6 | 336 | 166 | 498 | 2.9e-52 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28921.t1 | A0A392LXJ0 | 82.0 | 344 | 61 | 1 | 1 | 343 | 124 | 467 | 6.2e-157 | 563.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052016 | MS.gene28921 | 0.8012 | 1.01E-48 | -1.69E-46 |
| MS.gene052037 | MS.gene28921 | 0.808984 | 2.37E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene28921 | 0.801775 | 7.68E-49 | -1.69E-46 |
| MS.gene052493 | MS.gene28921 | 0.807026 | 6.18E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene28921 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene28921 | 0.807605 | 4.66E-50 | -1.69E-46 |
| MS.gene055231 | MS.gene28921 | 0.807009 | 6.23E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene28921 | 0.806658 | 7.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAAGATTGCAAATATAT+TGG | 0.199246 | 4.2:-44484646 | MS.gene28921:CDS |
| ATTCTTAATATATTTCAAAT+AGG | 0.217263 | 4.2:+44483816 | None:intergenic |
| ATCCTCCGAAAATGCTTATA+AGG | 0.232878 | 4.2:+44483734 | None:intergenic |
| ATTAGAAAAGCATTTGCTTT+TGG | 0.249244 | 4.2:-44484565 | MS.gene28921:CDS |
| TTTATCCCAACCAAATCTTT+TGG | 0.281846 | 4.2:+44484603 | None:intergenic |
| CATAAGATTGCAAATATATT+GGG | 0.293613 | 4.2:-44484645 | MS.gene28921:CDS |
| GTTGATGATGTTCGGGTTGT+AGG | 0.296065 | 4.2:-44484537 | MS.gene28921:CDS |
| TTCTTAATATATTTCAAATA+GGG | 0.301482 | 4.2:+44483817 | None:intergenic |
| TTCAAGTCTTCCAAAAGATT+TGG | 0.303903 | 4.2:-44484613 | MS.gene28921:CDS |
| TTGTAGGACTTTGCGGGATG+GGG | 0.318026 | 4.2:-44484521 | MS.gene28921:CDS |
| TGGACTCAGTTGATGATGTT+CGG | 0.318560 | 4.2:-44484545 | MS.gene28921:CDS |
| GTTGTAGGACTTTGCGGGAT+GGG | 0.344513 | 4.2:-44484522 | MS.gene28921:CDS |
| AACATTGTCAAGAATTATAA+AGG | 0.359820 | 4.2:+44484275 | None:intergenic |
| TGTAGGACTTTGCGGGATGG+GGG | 0.366826 | 4.2:-44484520 | MS.gene28921:CDS |
| TATAAGTGATTATGAGAAGT+TGG | 0.372802 | 4.2:-44484058 | MS.gene28921:CDS |
| TTCCAGAGACGAGCATATCT+TGG | 0.373958 | 4.2:-44484178 | MS.gene28921:CDS |
| AGCATACTCAGTTATGCTAA+TGG | 0.374347 | 4.2:-44484030 | MS.gene28921:CDS |
| ATGATTTAAGCAAAATGTAT+AGG | 0.376185 | 4.2:-44484422 | MS.gene28921:CDS |
| CATAATTTGTTAAGAGAGTT+GGG | 0.388141 | 4.2:-44483700 | MS.gene28921:CDS |
| TTCGGGTTGTAGGACTTTGC+GGG | 0.394386 | 4.2:-44484527 | MS.gene28921:CDS |
| CCACTAGCAATTAAAGTATT+GGG | 0.399390 | 4.2:-44484003 | MS.gene28921:CDS |
| ACATCCATGATATCTTTGTT+TGG | 0.399882 | 4.2:+44483916 | None:intergenic |
| GTCTTCCAAAAGATTTGGTT+GGG | 0.402134 | 4.2:-44484608 | MS.gene28921:CDS |
| GCATAATTTGTTAAGAGAGT+TGG | 0.403442 | 4.2:-44483701 | MS.gene28921:CDS |
| TGTGGATTTCACGCTGATAT+TGG | 0.403921 | 4.2:-44483781 | MS.gene28921:CDS |
| GAGCATATCTTGGAAGAGTA+TGG | 0.406942 | 4.2:-44484168 | MS.gene28921:CDS |
| GGACTCAGTTGATGATGTTC+GGG | 0.407672 | 4.2:-44484544 | MS.gene28921:CDS |
| GTTCGGGTTGTAGGACTTTG+CGG | 0.424502 | 4.2:-44484528 | MS.gene28921:CDS |
| ACCACTAGCAATTAAAGTAT+TGG | 0.426193 | 4.2:-44484004 | MS.gene28921:CDS |
| TCCTCCGAAAATGCTTATAA+GGG | 0.442418 | 4.2:+44483735 | None:intergenic |
| TTCCAAGATATGCTCGTCTC+TGG | 0.452730 | 4.2:+44484176 | None:intergenic |
| GTAGGACTTTGCGGGATGGG+GGG | 0.468589 | 4.2:-44484519 | MS.gene28921:CDS |
| AACTCCAAACAAAGATATCA+TGG | 0.470807 | 4.2:-44483920 | MS.gene28921:CDS |
| GGTTGTAGGACTTTGCGGGA+TGG | 0.477624 | 4.2:-44484523 | MS.gene28921:CDS |
| AAGAATATTCTAAATTGTTG+TGG | 0.485635 | 4.2:-44483799 | MS.gene28921:CDS |
| CATCTAACTTGATACGAAGT+AGG | 0.490909 | 4.2:-44484314 | MS.gene28921:CDS |
| AATTAGTTGTGAGTCGTGAA+TGG | 0.492227 | 4.2:-44484230 | MS.gene28921:CDS |
| AGTCTTCCAAAAGATTTGGT+TGG | 0.501116 | 4.2:-44484609 | MS.gene28921:CDS |
| AGCAAAATGTATAGGCATGA+TGG | 0.503930 | 4.2:-44484414 | MS.gene28921:CDS |
| CGCTGAATGGAAAAGTGCAT+TGG | 0.520229 | 4.2:-44483956 | MS.gene28921:CDS |
| CAAATTCTACTTCAAACTCT+AGG | 0.527789 | 4.2:-44484372 | MS.gene28921:CDS |
| TGGGCTAGAAGAAATGGAAA+AGG | 0.537019 | 4.2:-44483875 | MS.gene28921:CDS |
| AGTCGTGAATGGTTAGGTGC+GGG | 0.542032 | 4.2:-44484219 | MS.gene28921:CDS |
| TTGGTCGAGACATCGCTGAA+TGG | 0.549605 | 4.2:-44483969 | MS.gene28921:CDS |
| AAGTAGGCTACAACATGTAA+AGG | 0.552340 | 4.2:-44484298 | MS.gene28921:CDS |
| CCCAATACTTTAATTGCTAG+TGG | 0.553005 | 4.2:+44484003 | None:intergenic |
| GAGTTAGTCTTATTCAAGAG+TGG | 0.571852 | 4.2:+44484123 | None:intergenic |
| ATACTTTAATTGCTAGTGGT+AGG | 0.581922 | 4.2:+44484007 | None:intergenic |
| GTTGTGAGTCGTGAATGGTT+AGG | 0.593107 | 4.2:-44484225 | MS.gene28921:CDS |
| GCGAAGAGCAACTTCAGACA+TGG | 0.620417 | 4.2:-44484350 | MS.gene28921:CDS |
| TCATCAATAATGCTACACAC+TGG | 0.634283 | 4.2:+44484441 | None:intergenic |
| GTCGTGAATGGTTAGGTGCG+GGG | 0.638200 | 4.2:-44484218 | MS.gene28921:CDS |
| GAGTCGTGAATGGTTAGGTG+CGG | 0.650686 | 4.2:-44484220 | MS.gene28921:CDS |
| TATCTTGGAAGAGTATGGAG+TGG | 0.665247 | 4.2:-44484163 | MS.gene28921:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAATATATTTCAAAT+AGG | + | chr4.2:44484530-44484549 | None:intergenic | 10.0% |
| !! | TTCTTAATATATTTCAAATA+GGG | + | chr4.2:44484529-44484548 | None:intergenic | 10.0% |
| !! | AACATTGTCAAGAATTATAA+AGG | + | chr4.2:44484071-44484090 | None:intergenic | 20.0% |
| !! | AAGAATATTCTAAATTGTTG+TGG | - | chr4.2:44484544-44484563 | MS.gene28921:CDS | 20.0% |
| !! | ATGATTTAAGCAAAATGTAT+AGG | - | chr4.2:44483921-44483940 | MS.gene28921:CDS | 20.0% |
| !! | CATAAGATTGCAAATATATT+GGG | - | chr4.2:44483698-44483717 | MS.gene28921:CDS | 20.0% |
| !! | TCATAAGATTGCAAATATAT+TGG | - | chr4.2:44483697-44483716 | MS.gene28921:CDS | 20.0% |
| ! | TATAAGTGATTATGAGAAGT+TGG | - | chr4.2:44484285-44484304 | MS.gene28921:CDS | 25.0% |
| !! | AAATCCCTTATAAGCATTTT+CGG | - | chr4.2:44484604-44484623 | MS.gene28921:CDS | 25.0% |
| !! | TGCTTTTCTAATTCTTCTAT+AGG | + | chr4.2:44483770-44483789 | None:intergenic | 25.0% |
| !! | TTTTCAATCTCTACATATTG+TGG | + | chr4.2:44483671-44483690 | None:intergenic | 25.0% |
| !!! | ATTAGAAAAGCATTTGCTTT+TGG | - | chr4.2:44483778-44483797 | MS.gene28921:CDS | 25.0% |
| !!! | CATAATTTGTTAAGAGAGTT+GGG | - | chr4.2:44484643-44484662 | MS.gene28921:CDS | 25.0% |
| AACTCCAAACAAAGATATCA+TGG | - | chr4.2:44484423-44484442 | MS.gene28921:CDS | 30.0% | |
| ACATCCATGATATCTTTGTT+TGG | + | chr4.2:44484430-44484449 | None:intergenic | 30.0% | |
| ACCACTAGCAATTAAAGTAT+TGG | - | chr4.2:44484339-44484358 | MS.gene28921:CDS | 30.0% | |
| ATACTTTAATTGCTAGTGGT+AGG | + | chr4.2:44484339-44484358 | None:intergenic | 30.0% | |
| CAAATTCTACTTCAAACTCT+AGG | - | chr4.2:44483971-44483990 | MS.gene28921:CDS | 30.0% | |
| TTCAAGTCTTCCAAAAGATT+TGG | - | chr4.2:44483730-44483749 | MS.gene28921:CDS | 30.0% | |
| ! | CCACTAGCAATTAAAGTATT+GGG | - | chr4.2:44484340-44484359 | MS.gene28921:CDS | 30.0% |
| ! | TTTATCCCAACCAAATCTTT+TGG | + | chr4.2:44483743-44483762 | None:intergenic | 30.0% |
| !! | GCATAATTTGTTAAGAGAGT+TGG | - | chr4.2:44484642-44484661 | MS.gene28921:CDS | 30.0% |
| !! | TGTTGCGATTAAGTTTTGAT+GGG | - | chr4.2:44484449-44484468 | MS.gene28921:CDS | 30.0% |
| AAGTAGGCTACAACATGTAA+AGG | - | chr4.2:44484045-44484064 | MS.gene28921:CDS | 35.0% | |
| AATTAGTTGTGAGTCGTGAA+TGG | - | chr4.2:44484113-44484132 | MS.gene28921:CDS | 35.0% | |
| AGCAAAATGTATAGGCATGA+TGG | - | chr4.2:44483929-44483948 | MS.gene28921:CDS | 35.0% | |
| AGCATACTCAGTTATGCTAA+TGG | - | chr4.2:44484313-44484332 | MS.gene28921:CDS | 35.0% | |
| ATCCTCCGAAAATGCTTATA+AGG | + | chr4.2:44484612-44484631 | None:intergenic | 35.0% | |
| CATCTAACTTGATACGAAGT+AGG | - | chr4.2:44484029-44484048 | MS.gene28921:CDS | 35.0% | |
| CCCAATACTTTAATTGCTAG+TGG | + | chr4.2:44484343-44484362 | None:intergenic | 35.0% | |
| TCATCAATAATGCTACACAC+TGG | + | chr4.2:44483905-44483924 | None:intergenic | 35.0% | |
| TCCTCCGAAAATGCTTATAA+GGG | + | chr4.2:44484611-44484630 | None:intergenic | 35.0% | |
| ! | AGTCTTCCAAAAGATTTGGT+TGG | - | chr4.2:44483734-44483753 | MS.gene28921:CDS | 35.0% |
| ! | GTCTTCCAAAAGATTTGGTT+GGG | - | chr4.2:44483735-44483754 | MS.gene28921:CDS | 35.0% |
| !! | GAGTTAGTCTTATTCAAGAG+TGG | + | chr4.2:44484223-44484242 | None:intergenic | 35.0% |
| !! | GTATTGGGCTCATTTTTGTT+TGG | - | chr4.2:44484355-44484374 | MS.gene28921:CDS | 35.0% |
| !! | GTGTTGCGATTAAGTTTTGA+TGG | - | chr4.2:44484448-44484467 | MS.gene28921:CDS | 35.0% |
| !!! | TTTTGATGGGCTAGAAGAAA+TGG | - | chr4.2:44484462-44484481 | MS.gene28921:CDS | 35.0% |
| GAGCATATCTTGGAAGAGTA+TGG | - | chr4.2:44484175-44484194 | MS.gene28921:CDS | 40.0% | |
| TGTGGATTTCACGCTGATAT+TGG | - | chr4.2:44484562-44484581 | MS.gene28921:CDS | 40.0% | |
| ! | TATCTTGGAAGAGTATGGAG+TGG | - | chr4.2:44484180-44484199 | MS.gene28921:CDS | 40.0% |
| ! | TCCCTTATAAGCATTTTCGG+AGG | - | chr4.2:44484607-44484626 | MS.gene28921:CDS | 40.0% |
| ! | TGGGCTAGAAGAAATGGAAA+AGG | - | chr4.2:44484468-44484487 | MS.gene28921:CDS | 40.0% |
| !! | ATTTGCTTTTGTGCACTGAC+TGG | + | chr4.2:44483956-44483975 | None:intergenic | 40.0% |
| !! | TGGACTCAGTTGATGATGTT+CGG | - | chr4.2:44483798-44483817 | MS.gene28921:CDS | 40.0% |
| GTTGTGAGTCGTGAATGGTT+AGG | - | chr4.2:44484118-44484137 | MS.gene28921:CDS | 45.0% | |
| TTCCAAGATATGCTCGTCTC+TGG | + | chr4.2:44484170-44484189 | None:intergenic | 45.0% | |
| TTCCAGAGACGAGCATATCT+TGG | - | chr4.2:44484165-44484184 | MS.gene28921:CDS | 45.0% | |
| ! | CGCTGAATGGAAAAGTGCAT+TGG | - | chr4.2:44484387-44484406 | MS.gene28921:CDS | 45.0% |
| !! | GGACTCAGTTGATGATGTTC+GGG | - | chr4.2:44483799-44483818 | MS.gene28921:CDS | 45.0% |
| !! | GTTGATGATGTTCGGGTTGT+AGG | - | chr4.2:44483806-44483825 | MS.gene28921:CDS | 45.0% |
| AGTCGTGAATGGTTAGGTGC+GGG | - | chr4.2:44484124-44484143 | MS.gene28921:CDS | 50.0% | |
| GAGTCGTGAATGGTTAGGTG+CGG | - | chr4.2:44484123-44484142 | MS.gene28921:CDS | 50.0% | |
| GCGAAGAGCAACTTCAGACA+TGG | - | chr4.2:44483993-44484012 | MS.gene28921:CDS | 50.0% | |
| GTTGTAGGACTTTGCGGGAT+GGG | - | chr4.2:44483821-44483840 | MS.gene28921:CDS | 50.0% | |
| TTGGTCGAGACATCGCTGAA+TGG | - | chr4.2:44484374-44484393 | MS.gene28921:CDS | 50.0% | |
| TTGTAGGACTTTGCGGGATG+GGG | - | chr4.2:44483822-44483841 | MS.gene28921:CDS | 50.0% | |
| !! | GTTCGGGTTGTAGGACTTTG+CGG | - | chr4.2:44483815-44483834 | MS.gene28921:CDS | 50.0% |
| !! | TTCGGGTTGTAGGACTTTGC+GGG | - | chr4.2:44483816-44483835 | MS.gene28921:CDS | 50.0% |
| GGTTGTAGGACTTTGCGGGA+TGG | - | chr4.2:44483820-44483839 | MS.gene28921:CDS | 55.0% | |
| GTCGTGAATGGTTAGGTGCG+GGG | - | chr4.2:44484125-44484144 | MS.gene28921:CDS | 55.0% | |
| TGTAGGACTTTGCGGGATGG+GGG | - | chr4.2:44483823-44483842 | MS.gene28921:CDS | 55.0% | |
| TTTGCGGGATGGGGGGAATA+GGG | - | chr4.2:44483831-44483850 | MS.gene28921:CDS | 55.0% | |
| CTTTGCGGGATGGGGGGAAT+AGG | - | chr4.2:44483830-44483849 | MS.gene28921:CDS | 60.0% | |
| GTAGGACTTTGCGGGATGGG+GGG | - | chr4.2:44483824-44483843 | MS.gene28921:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 44483667 | 44484698 | 44483667 | ID=MS.gene28921 |
| chr4.2 | mRNA | 44483667 | 44484698 | 44483667 | ID=MS.gene28921.t1;Parent=MS.gene28921 |
| chr4.2 | exon | 44483667 | 44484698 | 44483667 | ID=MS.gene28921.t1.exon1;Parent=MS.gene28921.t1 |
| chr4.2 | CDS | 44483667 | 44484698 | 44483667 | ID=cds.MS.gene28921.t1;Parent=MS.gene28921.t1 |
| Gene Sequence |
| Protein sequence |
