AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28966

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28966.t1 MTR_4g075250 95.904 415 13 2 1 415 1 411 0.0 809
MS.gene28966.t1 MTR_5g075880 65.414 133 45 1 28 159 38 170 6.15e-51 170
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28966.t1 AT3G12400 58.907 421 139 5 1 411 1 397 1.04e-162 463
MS.gene28966.t1 AT3G12400 58.852 418 138 5 1 408 1 394 1.87e-161 460
MS.gene28966.t1 AT3G12400 58.852 418 138 5 1 408 1 394 3.38e-161 461
MS.gene28966.t1 AT5G13860 57.246 414 127 6 1 411 1 367 1.03e-155 444
MS.gene28966.t1 AT2G38830 36.025 161 96 4 12 168 12 169 5.10e-25 105
MS.gene28966.t1 AT2G38830 28.947 228 125 7 190 386 96 317 2.79e-16 79.7

Find 155 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATCAAAGTTCTAATTT+TGG 0.132572 4.4:+34830644 MS.gene28966:CDS
CTGAGGAATTGTTGGGTTTC+GGG 0.148763 4.4:-34830113 None:intergenic
CTGTTCCTTATTTGCATAAT+TGG 0.167917 4.4:+34830468 MS.gene28966:CDS
TTAGAACTTTGATTGGTATT+TGG 0.178300 4.4:-34830638 None:intergenic
GATTTACCCTAGTTCTAATT+TGG 0.178763 4.4:+34830490 MS.gene28966:CDS
AATTGTTGGGTTTCGGGGTT+TGG 0.182716 4.4:-34830107 None:intergenic
CATCCTCATGTTAATCCTTC+TGG 0.204058 4.4:+34830437 MS.gene28966:CDS
CCCAATTGTTATCTGGCTTA+TGG 0.211522 4.4:+34830346 MS.gene28966:CDS
ATTGTTGGGTTTCGGGGTTT+GGG 0.228262 4.4:-34830106 None:intergenic
GTGAGTGTAGGTGGCGGTTT+TGG 0.235521 4.4:-34830246 None:intergenic
GCTGAGGAATTGTTGGGTTT+CGG 0.263335 4.4:-34830114 None:intergenic
TGCTTTAGATAAGGGTGTTC+AGG 0.272232 4.4:+34831162 MS.gene28966:CDS
GGAGAATCAAGGGAAGAAAA+TGG 0.279130 4.4:+34831036 MS.gene28966:CDS
GCAGGCTCAGGTTGCGAATA+TGG 0.279429 4.4:+34831288 MS.gene28966:CDS
TAGATAAGGGTGTTCAGGTT+GGG 0.287353 4.4:+34831167 MS.gene28966:CDS
CGAGATGGCGCAGGGGAATA+AGG 0.290860 4.4:-34830749 None:intergenic
TAAATCCAATTATGCAAATA+AGG 0.295767 4.4:-34830473 None:intergenic
AGGGAAGAAAATGGGGAGTT+TGG 0.299911 4.4:+34831045 MS.gene28966:CDS
CTTTGATTGGTATTTGGATT+AGG 0.305114 4.4:-34830632 None:intergenic
TGATTGTACTTCTGCTGATT+TGG 0.313474 4.4:+34831120 MS.gene28966:CDS
TTGTTGGGTTTCGGGGTTTG+GGG 0.329175 4.4:-34830105 None:intergenic
ACAACATCCCAATTGTTATC+TGG 0.333242 4.4:+34830339 MS.gene28966:CDS
TTAGGGTTAGGGTTAGGATT+AGG 0.335904 4.4:-34830590 None:intergenic
GAGAATCAAGGGAAGAAAAT+GGG 0.338918 4.4:+34831037 MS.gene28966:CDS
TTTGATGAATACGGATGTTC+TGG 0.345033 4.4:+34831000 MS.gene28966:CDS
TTAGGATTAGGATTGGGATT+AGG 0.352631 4.4:-34830578 None:intergenic
TCCAAAATTAGAACTTTGAT+TGG 0.354530 4.4:-34830645 None:intergenic
GGTGAAATGGAGGGGTTGTT+TGG 0.355099 4.4:+34830878 MS.gene28966:CDS
TTTGGAACTAATCCTCCATC+TGG 0.359655 4.4:+34830662 MS.gene28966:CDS
AGTTAGAGCTGCTCAGTTGC+AGG 0.360251 4.4:+34831270 MS.gene28966:CDS
ACGCTTTATGCTTTAGATAA+GGG 0.364897 4.4:+34831154 MS.gene28966:CDS
TCCATAAGCCAGATAACAAT+TGG 0.369758 4.4:-34830347 None:intergenic
GCAAGATGAGATGGAGGGTT+TGG 0.370726 4.4:+34830958 MS.gene28966:CDS
GAAGACTAGAGAAGGTGAAA+TGG 0.378989 4.4:+34830865 MS.gene28966:CDS
AGCTGCTCAGTTGCAGGCTC+AGG 0.393395 4.4:+34831276 MS.gene28966:CDS
AGATAAGGGTGTTCAGGTTG+GGG 0.397945 4.4:+34831168 MS.gene28966:CDS
TTTGATGAGGATTAGGGTTA+GGG 0.399829 4.4:-34830601 None:intergenic
CATTTGGTGTCTTCGGAGTA+AGG 0.400855 4.4:-34830170 None:intergenic
TACGCTTTATGCTTTAGATA+AGG 0.408278 4.4:+34831153 MS.gene28966:CDS
ATGTTCTGGAGGGTTGGTTG+AGG 0.410858 4.4:+34831014 MS.gene28966:CDS
GAGAGCGGAGGGGCCGCGTT+GGG 0.413398 4.4:-34830147 None:intergenic
GGATTGAGCTGAGGAATTGT+TGG 0.417142 4.4:-34830121 None:intergenic
TCATGTTAATCCTTCTGGAT+TGG 0.419158 4.4:+34830442 MS.gene28966:CDS
GCTGCTAGGAATCATCATTA+TGG 0.423124 4.4:+34831310 MS.gene28966:CDS
TGAGGATTAGGGTTAGGGTT+AGG 0.423347 4.4:-34830596 None:intergenic
GGTATTGAAGCAGAGGGAGG+AGG 0.425612 4.4:+34830910 MS.gene28966:CDS
GAGGGGTTGTTTGGTTTGCA+AGG 0.429772 4.4:+34830887 MS.gene28966:CDS
AGTACAATCAAGCATCTGTT+TGG 0.430905 4.4:-34831107 None:intergenic
TACCGTTTGATCAGTATTTG+AGG 0.441585 4.4:+34831194 MS.gene28966:CDS
GGAAGGTGGTGAGGAGAGAG+AGG 0.450712 4.4:-34830211 None:intergenic
GATTGAGCTGAGGAATTGTT+GGG 0.455019 4.4:-34830120 None:intergenic
AGGGGTTGTTTGGTTTGCAA+GGG 0.461101 4.4:+34830888 MS.gene28966:CDS
CTCCTCAAATACTGATCAAA+CGG 0.461994 4.4:-34831196 None:intergenic
CGGAGGGGCCGCGTTGGGAG+AGG 0.462809 4.4:-34830142 None:intergenic
GCCATCTCGGCCTACGCATA+CGG 0.464143 4.4:+34830763 MS.gene28966:CDS
GGAGAGAGAGGAGATGTTGA+CGG 0.466235 4.4:-34830199 None:intergenic
TGTTCTGGAGGGTTGGTTGA+GGG 0.466933 4.4:+34831015 MS.gene28966:CDS
GATAAGCCATTTGGTGTCTT+CGG 0.468483 4.4:-34830177 None:intergenic
GTTAGGGTTAGGATTAGGAT+TGG 0.469730 4.4:-34830585 None:intergenic
TTAGGGTTAGGATTAGGATT+GGG 0.470431 4.4:-34830584 None:intergenic
CTTATTCCCCTGCGCCATCT+CGG 0.471542 4.4:+34830750 MS.gene28966:CDS
GGAGATGCAAGATGAGATGG+AGG 0.478519 4.4:+34830952 MS.gene28966:CDS
TTTAATAATCATATCCCTAG+TGG 0.481041 4.4:-34830408 None:intergenic
GACTAGAGAAGGTGAAATGG+AGG 0.483262 4.4:+34830868 MS.gene28966:CDS
GGATTGTGCCATCGGCTTGA+AGG 0.490820 4.4:-34830292 None:intergenic
CGAGAGAAGGGAAGGTGGTG+AGG 0.490894 4.4:-34830220 None:intergenic
TTAGATAAGGGTGTTCAGGT+TGG 0.491802 4.4:+34831166 MS.gene28966:CDS
GGGGTTGTTTGGTTTGCAAG+GGG 0.495332 4.4:+34830889 MS.gene28966:CDS
GGTTGGTTGAGGGAGAATCA+AGG 0.499129 4.4:+34831025 MS.gene28966:CDS
TGCAAGGGGTATTGAAGCAG+AGG 0.500833 4.4:+34830903 MS.gene28966:CDS
GGAGAGCGGAGGGGCCGCGT+TGG 0.502152 4.4:-34830148 None:intergenic
CCATCTGGGTATATTCATCC+TGG 0.504818 4.4:+34830677 MS.gene28966:CDS
TCGGAGTAAGGGAGAGCGGA+GGG 0.508533 4.4:-34830158 None:intergenic
ATGTTGACGGATAAGCCATT+TGG 0.510171 4.4:-34830186 None:intergenic
GGTCATGGGGATTGTGCCAT+CGG 0.512307 4.4:-34830300 None:intergenic
AGATCAACCAAATTAGAACT+AGG 0.514058 4.4:-34830497 None:intergenic
GTTGGTTGAGGGAGAATCAA+GGG 0.518369 4.4:+34831026 MS.gene28966:CDS
CTTACTCCGAAGACACCAAA+TGG 0.520060 4.4:+34830171 MS.gene28966:CDS
CTATTACTATTAATCCTACC+AGG 0.522290 4.4:-34830695 None:intergenic
TGCGTAGGCCGAGATGGCGC+AGG 0.522751 4.4:-34830758 None:intergenic
GAGTGTGAGGGCGTTGTCGA+GGG 0.528924 4.4:+34831216 MS.gene28966:CDS
TGTTGGGTTTCGGGGTTTGG+GGG 0.530033 4.4:-34830104 None:intergenic
CCATAAGCCAGATAACAATT+GGG 0.531355 4.4:-34830346 None:intergenic
GTGACGGATTGGTAGGTCAT+GGG 0.535180 4.4:-34830314 None:intergenic
GAAGGAGATGCAAGATGAGA+TGG 0.537662 4.4:+34830949 MS.gene28966:CDS
AGAATCAAGGGAAGAAAATG+GGG 0.538001 4.4:+34831038 MS.gene28966:CDS
GGGTTCGAGAGAAGGGAAGG+TGG 0.544802 4.4:-34830225 None:intergenic
ACTAGAGAAGGTGAAATGGA+GGG 0.549010 4.4:+34830869 MS.gene28966:CDS
TTTGGGTTCGAGAGAAGGGA+AGG 0.549016 4.4:-34830228 None:intergenic
TCCGTATGCGTAGGCCGAGA+TGG 0.550136 4.4:-34830764 None:intergenic
GTCAATCTCCTTCAAGCCGA+TGG 0.554176 4.4:+34830284 MS.gene28966:CDS
CTAGAGAAGGTGAAATGGAG+GGG 0.556264 4.4:+34830870 MS.gene28966:CDS
TTTGGAGCAGCAGTTGCAGA+TGG 0.557427 4.4:+34830976 MS.gene28966:CDS
GAGGAATGCTATTAACAAGC+TGG 0.557578 4.4:+34830808 MS.gene28966:CDS
GCAGTTCTTCCACCGTGCTA+CGG 0.559208 4.4:+34831240 MS.gene28966:CDS
ACGGCCGTCGTTGTGAGTGT+AGG 0.560561 4.4:-34830258 None:intergenic
GGGGAGTTTGGAGAATGTTG+AGG 0.560828 4.4:+34831057 MS.gene28966:CDS
ATCAGTATTTGAGGAGTGTG+AGG 0.563552 4.4:+34831203 MS.gene28966:CDS
TTAATAATCATATCCCTAGT+GGG 0.565302 4.4:-34830407 None:intergenic
GATCAACCAAATTAGAACTA+GGG 0.566055 4.4:-34830496 None:intergenic
GGAGTGTGAGGGCGTTGTCG+AGG 0.566472 4.4:+34831215 MS.gene28966:CDS
GGATGTTGTATGTGACGGAT+TGG 0.574702 4.4:-34830325 None:intergenic
GTGTTTATGTTAATCCCACT+AGG 0.576064 4.4:+34830393 MS.gene28966:CDS
GCCGTCGTTGTGAGTGTAGG+TGG 0.582716 4.4:-34830255 None:intergenic
TGAGGAATTGTTGGGTTTCG+GGG 0.583538 4.4:-34830112 None:intergenic
GAGTTTGGAGAATGTTGAGG+AGG 0.585981 4.4:+34831060 MS.gene28966:CDS
ATTTGGTGTCTTCGGAGTAA+GGG 0.586991 4.4:-34830169 None:intergenic
TTGGAACTAATCCTCCATCT+GGG 0.587137 4.4:+34830663 MS.gene28966:CDS
AGGTTGCGAATATGGCTGCT+AGG 0.587435 4.4:+34831296 MS.gene28966:CDS
GCGTAGGCCGAGATGGCGCA+GGG 0.587625 4.4:-34830757 None:intergenic
GATGAATACGGATGTTCTGG+AGG 0.588358 4.4:+34831003 MS.gene28966:CDS
ATCGGCTTGAAGGAGATTGA+CGG 0.588553 4.4:-34830282 None:intergenic
GCAGGGGAATAAGGCGAAGG+AGG 0.592529 4.4:-34830740 None:intergenic
ATAACGATGTGACGGCGTTG+AGG 0.593588 4.4:+34830843 MS.gene28966:CDS
TATTAACAAGCTGGTGGAGA+TGG 0.597232 4.4:+34830817 MS.gene28966:CDS
GTTGTATGTGACGGATTGGT+AGG 0.598464 4.4:-34830321 None:intergenic
TGTTTATGTTAATCCCACTA+GGG 0.610790 4.4:+34830394 MS.gene28966:CDS
TGTGACGGATTGGTAGGTCA+TGG 0.615822 4.4:-34830315 None:intergenic
TTCGGAGTAAGGGAGAGCGG+AGG 0.618360 4.4:-34830159 None:intergenic
GGAACAGAAACCAATCCAGA+AGG 0.619765 4.4:-34830452 None:intergenic
AATCATCATTATGGTAGCTG+AGG 0.620092 4.4:+34831319 MS.gene28966:CDS
ATACGGATGTTCTGGAGGGT+TGG 0.622239 4.4:+34831008 MS.gene28966:CDS
TAAACACGAGGAGGGTGACG+AGG 0.622293 4.4:-34830377 None:intergenic
GTCGTTGTGAGTGTAGGTGG+CGG 0.624469 4.4:-34830252 None:intergenic
TAACTTTAGCAGCCGTAGCA+CGG 0.627369 4.4:-34831252 None:intergenic
AGGGGTATTGAAGCAGAGGG+AGG 0.628332 4.4:+34830907 MS.gene28966:CDS
GTCTTCGGAGTAAGGGAGAG+CGG 0.628756 4.4:-34830162 None:intergenic
CTTGAAGGAGATTGACGGCA+CGG 0.628941 4.4:-34830277 None:intergenic
GCGTTGAGGAAGACTAGAGA+AGG 0.630568 4.4:+34830857 MS.gene28966:CDS
CTGGGTATATTCATCCTGGT+AGG 0.631659 4.4:+34830681 MS.gene28966:CDS
GAATGCTATTAACAAGCTGG+TGG 0.633228 4.4:+34830811 MS.gene28966:CDS
GGCGCAGGGGAATAAGGCGA+AGG 0.637625 4.4:-34830743 None:intergenic
TGACGGATTGGTAGGTCATG+GGG 0.639394 4.4:-34830313 None:intergenic
GCCACCTACACTCACAACGA+CGG 0.639409 4.4:+34830254 MS.gene28966:CDS
CCAGGATGAATATACCCAGA+TGG 0.642839 4.4:-34830677 None:intergenic
TCAGTATTTGAGGAGTGTGA+GGG 0.644098 4.4:+34831204 MS.gene28966:CDS
AATTGGGATGTTGTATGTGA+CGG 0.646465 4.4:-34830330 None:intergenic
GTAGGATCCTCCGTATGCGT+AGG 0.651752 4.4:-34830773 None:intergenic
GAGATGCAAGATGAGATGGA+GGG 0.654104 4.4:+34830953 MS.gene28966:CDS
ATGAATACGGATGTTCTGGA+GGG 0.655370 4.4:+34831004 MS.gene28966:CDS
GGATTAACATAAACACGAGG+AGG 0.659983 4.4:-34830386 None:intergenic
ATCTCGGCCTACGCATACGG+AGG 0.661157 4.4:+34830766 MS.gene28966:CDS
GCAAGGGGTATTGAAGCAGA+GGG 0.664131 4.4:+34830904 MS.gene28966:CDS
AAACACGAGGAGGGTGACGA+GGG 0.665903 4.4:-34830376 None:intergenic
CGGAGTAAGGGAGAGCGGAG+GGG 0.668045 4.4:-34830157 None:intergenic
AATCCAGAAGGATTAACATG+AGG 0.672810 4.4:-34830440 None:intergenic
GGATGAATATACCCAGATGG+AGG 0.673938 4.4:-34830674 None:intergenic
GTTGGGAGAGGATTGAGCTG+AGG 0.683317 4.4:-34830130 None:intergenic
CGTAGGCCGAGATGGCGCAG+GGG 0.702882 4.4:-34830756 None:intergenic
AGCTCAATCCTCTCCCAACG+CGG 0.705583 4.4:+34830134 MS.gene28966:CDS
GATTAACATAAACACGAGGA+GGG 0.706994 4.4:-34830385 None:intergenic
GATGGTGCATAACGATGTGA+CGG 0.707755 4.4:+34830835 MS.gene28966:CDS
CTTTAGCAGCCGTAGCACGG+TGG 0.725711 4.4:-34831249 None:intergenic
GCATACGGAGGATCCTACTG+AGG 0.760419 4.4:+34830778 MS.gene28966:CDS
GTGGGATTAACATAAACACG+AGG 0.780290 4.4:-34830389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATCCAATTATGCAAATA+AGG - chr4.4:34830476-34830495 None:intergenic 20.0%
! TCCAAAATTAGAACTTTGAT+TGG - chr4.4:34830648-34830667 None:intergenic 25.0%
! TTAATAATCATATCCCTAGT+GGG - chr4.4:34830410-34830429 None:intergenic 25.0%
! TTAGAACTTTGATTGGTATT+TGG - chr4.4:34830641-34830660 None:intergenic 25.0%
! TTTAATAATCATATCCCTAG+TGG - chr4.4:34830411-34830430 None:intergenic 25.0%
!!! ACCAATCAAAGTTCTAATTT+TGG + chr4.4:34830644-34830663 MS.gene28966:CDS 25.0%
!!! TATGATTTTGATGAGGATTA+GGG - chr4.4:34830610-34830629 None:intergenic 25.0%
!!! TTAGGATTATGATTTTGATG+AGG - chr4.4:34830617-34830636 None:intergenic 25.0%
!!! TTATGATTTTGATGAGGATT+AGG - chr4.4:34830611-34830630 None:intergenic 25.0%
AGATCAACCAAATTAGAACT+AGG - chr4.4:34830500-34830519 None:intergenic 30.0%
CTATTACTATTAATCCTACC+AGG - chr4.4:34830698-34830717 None:intergenic 30.0%
CTTTGATTGGTATTTGGATT+AGG - chr4.4:34830635-34830654 None:intergenic 30.0%
GATCAACCAAATTAGAACTA+GGG - chr4.4:34830499-34830518 None:intergenic 30.0%
TGTTTATGTTAATCCCACTA+GGG + chr4.4:34830394-34830413 MS.gene28966:CDS 30.0%
! ACGCTTTATGCTTTAGATAA+GGG + chr4.4:34831154-34831173 MS.gene28966:CDS 30.0%
! CTGTTCCTTATTTGCATAAT+TGG + chr4.4:34830468-34830487 MS.gene28966:CDS 30.0%
! TACGCTTTATGCTTTAGATA+AGG + chr4.4:34831153-34831172 MS.gene28966:CDS 30.0%
!! CTTGCTTTGAGTTTGATTTT+TGG + chr4.4:34830524-34830543 MS.gene28966:CDS 30.0%
!! GATTTACCCTAGTTCTAATT+TGG + chr4.4:34830490-34830509 MS.gene28966:CDS 30.0%
AATCCAGAAGGATTAACATG+AGG - chr4.4:34830443-34830462 None:intergenic 35.0%
AATTGGGATGTTGTATGTGA+CGG - chr4.4:34830333-34830352 None:intergenic 35.0%
ACAACATCCCAATTGTTATC+TGG + chr4.4:34830339-34830358 MS.gene28966:CDS 35.0%
AGAATCAAGGGAAGAAAATG+GGG + chr4.4:34831038-34831057 MS.gene28966:CDS 35.0%
AGTACAATCAAGCATCTGTT+TGG - chr4.4:34831110-34831129 None:intergenic 35.0%
CCATAAGCCAGATAACAATT+GGG - chr4.4:34830349-34830368 None:intergenic 35.0%
CTCCTCAAATACTGATCAAA+CGG - chr4.4:34831199-34831218 None:intergenic 35.0%
GAGAATCAAGGGAAGAAAAT+GGG + chr4.4:34831037-34831056 MS.gene28966:CDS 35.0%
GATTAACATAAACACGAGGA+GGG - chr4.4:34830388-34830407 None:intergenic 35.0%
GTGTTTATGTTAATCCCACT+AGG + chr4.4:34830393-34830412 MS.gene28966:CDS 35.0%
TACCGTTTGATCAGTATTTG+AGG + chr4.4:34831194-34831213 MS.gene28966:CDS 35.0%
TCATGTTAATCCTTCTGGAT+TGG + chr4.4:34830442-34830461 MS.gene28966:CDS 35.0%
TCCATAAGCCAGATAACAAT+TGG - chr4.4:34830350-34830369 None:intergenic 35.0%
TTAGGATTAGGATTGGGATT+AGG - chr4.4:34830581-34830600 None:intergenic 35.0%
TTAGGGTTAGGATTAGGATT+GGG - chr4.4:34830587-34830606 None:intergenic 35.0%
TTTGATGAATACGGATGTTC+TGG + chr4.4:34831000-34831019 MS.gene28966:CDS 35.0%
TTTGATGAGGATTAGGGTTA+GGG - chr4.4:34830604-34830623 None:intergenic 35.0%
! TGATTGTACTTCTGCTGATT+TGG + chr4.4:34831120-34831139 MS.gene28966:CDS 35.0%
! TTTTGATGAGGATTAGGGTT+AGG - chr4.4:34830605-34830624 None:intergenic 35.0%
!!! GCAGATGGTTTTGATGAATA+CGG + chr4.4:34830991-34831010 MS.gene28966:CDS 35.0%
!!! GGTTTTGAATAATGGTGTGA+AGG + chr4.4:34830931-34830950 MS.gene28966:CDS 35.0%
ACTAGAGAAGGTGAAATGGA+GGG + chr4.4:34830869-34830888 MS.gene28966:CDS 40.0%
CATCCTCATGTTAATCCTTC+TGG + chr4.4:34830437-34830456 MS.gene28966:CDS 40.0%
CTACGTTGAGAAAAAAGAGG+TGG - chr4.4:34830557-34830576 None:intergenic 40.0%
GAAGACTAGAGAAGGTGAAA+TGG + chr4.4:34830865-34830884 MS.gene28966:CDS 40.0%
GAATGCTATTAACAAGCTGG+TGG + chr4.4:34830811-34830830 MS.gene28966:CDS 40.0%
GAGGAATGCTATTAACAAGC+TGG + chr4.4:34830808-34830827 MS.gene28966:CDS 40.0%
GATTGAGCTGAGGAATTGTT+GGG - chr4.4:34830123-34830142 None:intergenic 40.0%
GCTGCTAGGAATCATCATTA+TGG + chr4.4:34831310-34831329 MS.gene28966:CDS 40.0%
GGAGAATCAAGGGAAGAAAA+TGG + chr4.4:34831036-34831055 MS.gene28966:CDS 40.0%
GGATTAACATAAACACGAGG+AGG - chr4.4:34830389-34830408 None:intergenic 40.0%
GGTCTACGTTGAGAAAAAAG+AGG - chr4.4:34830560-34830579 None:intergenic 40.0%
GTGGGATTAACATAAACACG+AGG - chr4.4:34830392-34830411 None:intergenic 40.0%
GTTAGGGTTAGGATTAGGAT+TGG - chr4.4:34830588-34830607 None:intergenic 40.0%
TATTAACAAGCTGGTGGAGA+TGG + chr4.4:34830817-34830836 MS.gene28966:CDS 40.0%
TTAGGGTTAGGGTTAGGATT+AGG - chr4.4:34830593-34830612 None:intergenic 40.0%
TTCCTCCTAAAAACCTCAGT+AGG - chr4.4:34830794-34830813 None:intergenic 40.0%
TTGGAACTAATCCTCCATCT+GGG + chr4.4:34830663-34830682 MS.gene28966:CDS 40.0%
! AGGATCCTACTGAGGTTTTT+AGG + chr4.4:34830786-34830805 MS.gene28966:CDS 40.0%
! ATCAGTATTTGAGGAGTGTG+AGG + chr4.4:34831203-34831222 MS.gene28966:CDS 40.0%
! ATGAATACGGATGTTCTGGA+GGG + chr4.4:34831004-34831023 MS.gene28966:CDS 40.0%
! ATGTTGACGGATAAGCCATT+TGG - chr4.4:34830189-34830208 None:intergenic 40.0%
! CCCAATTGTTATCTGGCTTA+TGG + chr4.4:34830346-34830365 MS.gene28966:CDS 40.0%
! TCAGTATTTGAGGAGTGTGA+GGG + chr4.4:34831204-34831223 MS.gene28966:CDS 40.0%
! TTTGGAACTAATCCTCCATC+TGG + chr4.4:34830662-34830681 MS.gene28966:CDS 40.0%
!! ATCCTACTGAGGTTTTTAGG+AGG + chr4.4:34830789-34830808 MS.gene28966:CDS 40.0%
!! ATTTGGTGTCTTCGGAGTAA+GGG - chr4.4:34830172-34830191 None:intergenic 40.0%
!! GATAAGCCATTTGGTGTCTT+CGG - chr4.4:34830180-34830199 None:intergenic 40.0%
!! TAGATAAGGGTGTTCAGGTT+GGG + chr4.4:34831167-34831186 MS.gene28966:CDS 40.0%
!! TGCTTTAGATAAGGGTGTTC+AGG + chr4.4:34831162-34831181 MS.gene28966:CDS 40.0%
!! TTAGATAAGGGTGTTCAGGT+TGG + chr4.4:34831166-34831185 MS.gene28966:CDS 40.0%
!!! AGGGAGGAGGTTTTGAATAA+TGG + chr4.4:34830923-34830942 MS.gene28966:CDS 40.0%
AATTGTTGGGTTTCGGGGTT+TGG - chr4.4:34830110-34830129 None:intergenic 45.0%
AGGGAAGAAAATGGGGAGTT+TGG + chr4.4:34831045-34831064 MS.gene28966:CDS 45.0%
ATTGTTGGGTTTCGGGGTTT+GGG - chr4.4:34830109-34830128 None:intergenic 45.0%
CCAGGATGAATATACCCAGA+TGG - chr4.4:34830680-34830699 None:intergenic 45.0%
CCATCTGGGTATATTCATCC+TGG + chr4.4:34830677-34830696 MS.gene28966:CDS 45.0%
CTAGAGAAGGTGAAATGGAG+GGG + chr4.4:34830870-34830889 MS.gene28966:CDS 45.0%
CTGAGGAATTGTTGGGTTTC+GGG - chr4.4:34830116-34830135 None:intergenic 45.0%
CTGGGTATATTCATCCTGGT+AGG + chr4.4:34830681-34830700 MS.gene28966:CDS 45.0%
CTTACTCCGAAGACACCAAA+TGG + chr4.4:34830171-34830190 MS.gene28966:CDS 45.0%
GAAGGAGATGCAAGATGAGA+TGG + chr4.4:34830949-34830968 MS.gene28966:CDS 45.0%
GACTAGAGAAGGTGAAATGG+AGG + chr4.4:34830868-34830887 MS.gene28966:CDS 45.0%
GAGATGCAAGATGAGATGGA+GGG + chr4.4:34830953-34830972 MS.gene28966:CDS 45.0%
GAGTTTGGAGAATGTTGAGG+AGG + chr4.4:34831060-34831079 MS.gene28966:CDS 45.0%
GATGAATACGGATGTTCTGG+AGG + chr4.4:34831003-34831022 MS.gene28966:CDS 45.0%
GATGGTGCATAACGATGTGA+CGG + chr4.4:34830835-34830854 MS.gene28966:CDS 45.0%
GCTGAGGAATTGTTGGGTTT+CGG - chr4.4:34830117-34830136 None:intergenic 45.0%
GGAACAGAAACCAATCCAGA+AGG - chr4.4:34830455-34830474 None:intergenic 45.0%
GGATGAATATACCCAGATGG+AGG - chr4.4:34830677-34830696 None:intergenic 45.0%
GGATTGAGCTGAGGAATTGT+TGG - chr4.4:34830124-34830143 None:intergenic 45.0%
GTTGGTTGAGGGAGAATCAA+GGG + chr4.4:34831026-34831045 MS.gene28966:CDS 45.0%
TAACTTTAGCAGCCGTAGCA+CGG - chr4.4:34831255-34831274 None:intergenic 45.0%
TGAGGAATTGTTGGGTTTCG+GGG - chr4.4:34830115-34830134 None:intergenic 45.0%
TGAGGATTAGGGTTAGGGTT+AGG - chr4.4:34830599-34830618 None:intergenic 45.0%
!! AGATAAGGGTGTTCAGGTTG+GGG + chr4.4:34831168-34831187 MS.gene28966:CDS 45.0%
!! AGGGGTTGTTTGGTTTGCAA+GGG + chr4.4:34830888-34830907 MS.gene28966:CDS 45.0%
!! ATCGGCTTGAAGGAGATTGA+CGG - chr4.4:34830285-34830304 None:intergenic 45.0%
!! CATTTGGTGTCTTCGGAGTA+AGG - chr4.4:34830173-34830192 None:intergenic 45.0%
!! GGATGTTGTATGTGACGGAT+TGG - chr4.4:34830328-34830347 None:intergenic 45.0%
!! GTTGTATGTGACGGATTGGT+AGG - chr4.4:34830324-34830343 None:intergenic 45.0%
AGGTTGCGAATATGGCTGCT+AGG + chr4.4:34831296-34831315 MS.gene28966:CDS 50.0%
AGTTAGAGCTGCTCAGTTGC+AGG + chr4.4:34831270-34831289 MS.gene28966:CDS 50.0%
ATAACGATGTGACGGCGTTG+AGG + chr4.4:34830843-34830862 MS.gene28966:CDS 50.0%
CTTGAAGGAGATTGACGGCA+CGG - chr4.4:34830280-34830299 None:intergenic 50.0%
GCAAGATGAGATGGAGGGTT+TGG + chr4.4:34830958-34830977 MS.gene28966:CDS 50.0%
GCGTTGAGGAAGACTAGAGA+AGG + chr4.4:34830857-34830876 MS.gene28966:CDS 50.0%
GGAGAGAGAGGAGATGTTGA+CGG - chr4.4:34830202-34830221 None:intergenic 50.0%
GGAGATGCAAGATGAGATGG+AGG + chr4.4:34830952-34830971 MS.gene28966:CDS 50.0%
GGGGAGTTTGGAGAATGTTG+AGG + chr4.4:34831057-34831076 MS.gene28966:CDS 50.0%
GGTGAAATGGAGGGGTTGTT+TGG + chr4.4:34830878-34830897 MS.gene28966:CDS 50.0%
GTCAATCTCCTTCAAGCCGA+TGG + chr4.4:34830284-34830303 MS.gene28966:CDS 50.0%
TTGTTGGGTTTCGGGGTTTG+GGG - chr4.4:34830108-34830127 None:intergenic 50.0%
! GGTTGGTTGAGGGAGAATCA+AGG + chr4.4:34831025-34831044 MS.gene28966:CDS 50.0%
! TTTGGAGCAGCAGTTGCAGA+TGG + chr4.4:34830976-34830995 MS.gene28966:CDS 50.0%
! TTTGGGTTCGAGAGAAGGGA+AGG - chr4.4:34830231-34830250 None:intergenic 50.0%
!! ATACGGATGTTCTGGAGGGT+TGG + chr4.4:34831008-34831027 MS.gene28966:CDS 50.0%
!! ATGTTCTGGAGGGTTGGTTG+AGG + chr4.4:34831014-34831033 MS.gene28966:CDS 50.0%
!! GAGGGGTTGTTTGGTTTGCA+AGG + chr4.4:34830887-34830906 MS.gene28966:CDS 50.0%
!! GCAAGGGGTATTGAAGCAGA+GGG + chr4.4:34830904-34830923 MS.gene28966:CDS 50.0%
!! GGGGTTGTTTGGTTTGCAAG+GGG + chr4.4:34830889-34830908 MS.gene28966:CDS 50.0%
!! GTGACGGATTGGTAGGTCAT+GGG - chr4.4:34830317-34830336 None:intergenic 50.0%
!! TGACGGATTGGTAGGTCATG+GGG - chr4.4:34830316-34830335 None:intergenic 50.0%
!! TGAGGAGGCTTTTGAGTGTG+TGG + chr4.4:34831075-34831094 MS.gene28966:CDS 50.0%
!! TGAGTGTAGGTGGCGGTTTT+GGG - chr4.4:34830248-34830267 None:intergenic 50.0%
!! TGCAAGGGGTATTGAAGCAG+AGG + chr4.4:34830903-34830922 MS.gene28966:CDS 50.0%
!! TGTGACGGATTGGTAGGTCA+TGG - chr4.4:34830318-34830337 None:intergenic 50.0%
!! TGTTCTGGAGGGTTGGTTGA+GGG + chr4.4:34831015-34831034 MS.gene28966:CDS 50.0%
!!! CGGTTTTGGGTTCGAGAGAA+GGG - chr4.4:34830235-34830254 None:intergenic 50.0%
AAACACGAGGAGGGTGACGA+GGG - chr4.4:34830379-34830398 None:intergenic 55.0%
AGCTCAATCCTCTCCCAACG+CGG + chr4.4:34830134-34830153 MS.gene28966:CDS 55.0%
CTTATTCCCCTGCGCCATCT+CGG + chr4.4:34830750-34830769 MS.gene28966:CDS 55.0%
GCAGTTCTTCCACCGTGCTA+CGG + chr4.4:34831240-34831259 MS.gene28966:CDS 55.0%
GCATACGGAGGATCCTACTG+AGG + chr4.4:34830778-34830797 MS.gene28966:CDS 55.0%
GCCACCTACACTCACAACGA+CGG + chr4.4:34830254-34830273 MS.gene28966:CDS 55.0%
GGTCATGGGGATTGTGCCAT+CGG - chr4.4:34830303-34830322 None:intergenic 55.0%
GTAGGATCCTCCGTATGCGT+AGG - chr4.4:34830776-34830795 None:intergenic 55.0%
GTCGTTGTGAGTGTAGGTGG+CGG - chr4.4:34830255-34830274 None:intergenic 55.0%
GTCTTCGGAGTAAGGGAGAG+CGG - chr4.4:34830165-34830184 None:intergenic 55.0%
GTTGGGAGAGGATTGAGCTG+AGG - chr4.4:34830133-34830152 None:intergenic 55.0%
TAAACACGAGGAGGGTGACG+AGG - chr4.4:34830380-34830399 None:intergenic 55.0%
TGTTGGGTTTCGGGGTTTGG+GGG - chr4.4:34830107-34830126 None:intergenic 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG + chr4.4:34831288-34831307 MS.gene28966:CDS 55.0%
! GGTATTGAAGCAGAGGGAGG+AGG + chr4.4:34830910-34830929 MS.gene28966:CDS 55.0%
!! AGGGGTATTGAAGCAGAGGG+AGG + chr4.4:34830907-34830926 MS.gene28966:CDS 55.0%
!! GGATTGTGCCATCGGCTTGA+AGG - chr4.4:34830295-34830314 None:intergenic 55.0%
!! GTGAGTGTAGGTGGCGGTTT+TGG - chr4.4:34830249-34830268 None:intergenic 55.0%
!!! GCGGTTTTGGGTTCGAGAGA+AGG - chr4.4:34830236-34830255 None:intergenic 55.0%
ACGGCCGTCGTTGTGAGTGT+AGG - chr4.4:34830261-34830280 None:intergenic 60.0%
AGCTGCTCAGTTGCAGGCTC+AGG + chr4.4:34831276-34831295 MS.gene28966:CDS 60.0%
ATCTCGGCCTACGCATACGG+AGG + chr4.4:34830766-34830785 MS.gene28966:CDS 60.0%
CGAGAGAAGGGAAGGTGGTG+AGG - chr4.4:34830223-34830242 None:intergenic 60.0%
CGAGATGGCGCAGGGGAATA+AGG - chr4.4:34830752-34830771 None:intergenic 60.0%
CTTTAGCAGCCGTAGCACGG+TGG - chr4.4:34831252-34831271 None:intergenic 60.0%
GCAGGGGAATAAGGCGAAGG+AGG - chr4.4:34830743-34830762 None:intergenic 60.0%
GCCATCTCGGCCTACGCATA+CGG + chr4.4:34830763-34830782 MS.gene28966:CDS 60.0%
GCCGTCGTTGTGAGTGTAGG+TGG - chr4.4:34830258-34830277 None:intergenic 60.0%
GGAAGGTGGTGAGGAGAGAG+AGG - chr4.4:34830214-34830233 None:intergenic 60.0%
GGGTTCGAGAGAAGGGAAGG+TGG - chr4.4:34830228-34830247 None:intergenic 60.0%
TCCGTATGCGTAGGCCGAGA+TGG - chr4.4:34830767-34830786 None:intergenic 60.0%
TCGGAGTAAGGGAGAGCGGA+GGG - chr4.4:34830161-34830180 None:intergenic 60.0%
TTCGGAGTAAGGGAGAGCGG+AGG - chr4.4:34830162-34830181 None:intergenic 60.0%
!! GAGTGTGAGGGCGTTGTCGA+GGG + chr4.4:34831216-34831235 MS.gene28966:CDS 60.0%
CGGAGTAAGGGAGAGCGGAG+GGG - chr4.4:34830160-34830179 None:intergenic 65.0%
GGCGCAGGGGAATAAGGCGA+AGG - chr4.4:34830746-34830765 None:intergenic 65.0%
GTTTCGGGGTTTGGGGGTGG+TGG - chr4.4:34830101-34830120 None:intergenic 65.0%
TGGGTTTCGGGGTTTGGGGG+TGG - chr4.4:34830104-34830123 None:intergenic 65.0%
!! GGAGTGTGAGGGCGTTGTCG+AGG + chr4.4:34831215-34831234 MS.gene28966:CDS 65.0%
CGTAGGCCGAGATGGCGCAG+GGG - chr4.4:34830759-34830778 None:intergenic 70.0%
GCGTAGGCCGAGATGGCGCA+GGG - chr4.4:34830760-34830779 None:intergenic 70.0%
TGCGTAGGCCGAGATGGCGC+AGG - chr4.4:34830761-34830780 None:intergenic 70.0%
GAGAGCGGAGGGGCCGCGTT+GGG - chr4.4:34830150-34830169 None:intergenic 75.0%
! CGGAGGGGCCGCGTTGGGAG+AGG - chr4.4:34830145-34830164 None:intergenic 80.0%
! GGAGAGCGGAGGGGCCGCGT+TGG - chr4.4:34830151-34830170 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34830092 34831339 34830092 ID=MS.gene28966
chr4.4 mRNA 34830092 34831339 34830092 ID=MS.gene28966.t1;Parent=MS.gene28966
chr4.4 exon 34830092 34831339 34830092 ID=MS.gene28966.t1.exon1;Parent=MS.gene28966.t1
chr4.4 CDS 34830092 34831339 34830092 ID=cds.MS.gene28966.t1;Parent=MS.gene28966.t1
Gene Sequence

>MS.gene28966

ATGGTTCCACCACCCCCAAACCCCGAAACCCAACAATTCCTCAGCTCAATCCTCTCCCAACGCGGCCCCTCCGCTCTCCCTTACTCCGAAGACACCAAATGGCTTATCCGTCAACATCTCCTCTCTCTCCTCACCACCTTCCCTTCTCTCGAACCCAAAACCGCCACCTACACTCACAACGACGGCCGTGCCGTCAATCTCCTTCAAGCCGATGGCACAATCCCCATGACCTACCAATCCGTCACATACAACATCCCAATTGTTATCTGGCTTATGGAATCTTACCCTCGTCACCCTCCTCGTGTTTATGTTAATCCCACTAGGGATATGATTATTAAACATGCTCATCCTCATGTTAATCCTTCTGGATTGGTTTCTGTTCCTTATTTGCATAATTGGATTTACCCTAGTTCTAATTTGGTTGATCTTGTTCTTGCTTTGAGTTTGATTTTTGGTCGTGATCCACCTCTTTTTTCTCAACGTAGACCTAATCCCAATCCTAATCCTAACCCTAACCCTAATCCTCATCAAAATCATAATCCTAATCCAAATACCAATCAAAGTTCTAATTTTGGAACTAATCCTCCATCTGGGTATATTCATCCTGGTAGGATTAATAGTAATAGTAATATTAATATTAATAGTTATCCTCCTTCGCCTTATTCCCCTGCGCCATCTCGGCCTACGCATACGGAGGATCCTACTGAGGTTTTTAGGAGGAATGCTATTAACAAGCTGGTGGAGATGGTGCATAACGATGTGACGGCGTTGAGGAAGACTAGAGAAGGTGAAATGGAGGGGTTGTTTGGTTTGCAAGGGGTATTGAAGCAGAGGGAGGAGGTTTTGAATAATGGTGTGAAGGAGATGCAAGATGAGATGGAGGGTTTGGAGCAGCAGTTGCAGATGGTTTTGATGAATACGGATGTTCTGGAGGGTTGGTTGAGGGAGAATCAAGGGAAGAAAATGGGGAGTTTGGAGAATGTTGAGGAGGCTTTTGAGTGTGTGGATGTGCTTTCCAAACAGATGCTTGATTGTACTTCTGCTGATTTGGCTATTGAAGATACGCTTTATGCTTTAGATAAGGGTGTTCAGGTTGGGGCTGTACCGTTTGATCAGTATTTGAGGAGTGTGAGGGCGTTGTCGAGGGAGCAGTTCTTCCACCGTGCTACGGCTGCTAAAGTTAGAGCTGCTCAGTTGCAGGCTCAGGTTGCGAATATGGCTGCTAGGAATCATCATTATGGTAGCTGA
Protein sequence

>MS.gene28966.t1

MVPPPPNPETQQFLSSILSQRGPSALPYSEDTKWLIRQHLLSLLTTFPSLEPKTATYTHNDGRAVNLLQADGTIPMTYQSVTYNIPIVIWLMESYPRHPPRVYVNPTRDMIIKHAHPHVNPSGLVSVPYLHNWIYPSSNLVDLVLALSLIFGRDPPLFSQRRPNPNPNPNPNPNPHQNHNPNPNTNQSSNFGTNPPSGYIHPGRINSNSNININSYPPSPYSPAPSRPTHTEDPTEVFRRNAINKLVEMVHNDVTALRKTREGEMEGLFGLQGVLKQREEVLNNGVKEMQDEMEGLEQQLQMVLMNTDVLEGWLRENQGKKMGSLENVEEAFECVDVLSKQMLDCTSADLAIEDTLYALDKGVQVGAVPFDQYLRSVRALSREQFFHRATAAKVRAAQLQAQVANMAARNHHYGS