Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29628.t1 | XP_003616621.1 | 97.2 | 144 | 4 | 0 | 1 | 144 | 1 | 144 | 2.20E-77 | 298.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29628.t1 | Q9ST43 | 69.5 | 141 | 41 | 1 | 4 | 142 | 3 | 143 | 3.4e-55 | 215.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29628.t1 | G7KGZ7 | 97.2 | 144 | 4 | 0 | 1 | 144 | 1 | 144 | 1.6e-77 | 298.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29628 | MS.gene03201 | PPI |
| MS.gene29628 | MS.gene02572 | PPI |
| MS.gene29628 | MS.gene013786 | PPI |
| MS.gene29628 | MS.gene46264 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29628.t1 | MTR_5g082470 | 97.222 | 144 | 4 | 0 | 1 | 144 | 1 | 144 | 1.00e-103 | 292 |
| MS.gene29628.t1 | MTR_3g463520 | 70.213 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 5.93e-73 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29628.t1 | AT5G05710 | 79.433 | 141 | 28 | 1 | 3 | 142 | 2 | 142 | 5.44e-80 | 233 |
| MS.gene29628.t1 | AT2G29700 | 69.504 | 141 | 41 | 1 | 4 | 142 | 3 | 143 | 5.56e-71 | 210 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTACTTGTCTTACTGTTAA+AGG | 0.201576 | 5.1:-64556624 | MS.gene29628:CDS |
| GTTAACCAGCCGGTTCGTTC+TGG | 0.208272 | 5.1:+64556771 | None:intergenic |
| ACTACTCCGGCGTCGAATTC+TGG | 0.239180 | 5.1:-64556800 | MS.gene29628:CDS |
| TGAAACAAGGGAAACTCTTC+TGG | 0.272043 | 5.1:-64556701 | MS.gene29628:CDS |
| TTAACCAGCCGGTTCGTTCT+GGG | 0.377723 | 5.1:+64556772 | None:intergenic |
| GAAATTGTTGATTATGATAA+CGG | 0.378590 | 5.1:-64556447 | MS.gene29628:CDS |
| TTCTAGATCTGTTACTGATT+CGG | 0.390424 | 5.1:-64556469 | MS.gene29628:CDS |
| ACAGTAAGACAAGTAGCAAC+AGG | 0.406909 | 5.1:+64556630 | None:intergenic |
| GACCGGGTTTGTGGTTGAAC+CGG | 0.413116 | 5.1:+64556824 | None:intergenic |
| GAGGATTGGATTAACTCGAT+TGG | 0.460815 | 5.1:-64556510 | MS.gene29628:CDS |
| AGTCTGTGGCGCTACGCAAC+CGG | 0.467927 | 5.1:-64556843 | MS.gene29628:CDS |
| TGATTCTGATAAGGAGAAAG+AGG | 0.505978 | 5.1:-64556529 | MS.gene29628:CDS |
| CTGATAAGGAGAAAGAGGAT+TGG | 0.515649 | 5.1:-64556524 | MS.gene29628:CDS |
| TGGTCGAACCCAGAACGAAC+CGG | 0.518095 | 5.1:-64556780 | MS.gene29628:CDS |
| GGAGTAGTCGACCGGGTTTG+TGG | 0.532363 | 5.1:+64556815 | None:intergenic |
| ACAAACCCGGTCGACTACTC+CGG | 0.537859 | 5.1:-64556813 | MS.gene29628:CDS |
| CCGGAATGGCAGCGAGTCTG+TGG | 0.538111 | 5.1:-64556857 | None:intergenic |
| TCGACGCCGGAGTAGTCGAC+CGG | 0.566459 | 5.1:+64556807 | None:intergenic |
| AACCGGTTCAACCACAAACC+CGG | 0.568891 | 5.1:-64556826 | MS.gene29628:CDS |
| GTGGAAAGTTCAAACGAGTA+GGG | 0.571418 | 5.1:+64556579 | None:intergenic |
| AGTGGAAAGTTCAAACGAGT+AGG | 0.587441 | 5.1:+64556578 | None:intergenic |
| ATACATCGTATCAGCGCGAG+TGG | 0.592591 | 5.1:+64556560 | None:intergenic |
| CGAACCCAGAACGAACCGGC+TGG | 0.620445 | 5.1:-64556776 | MS.gene29628:CDS |
| CGACGCCGGAGTAGTCGACC+GGG | 0.622012 | 5.1:+64556808 | None:intergenic |
| CAGTAAGACAAGTAGCAACA+GGG | 0.658308 | 5.1:+64556631 | None:intergenic |
| GTTCGACCAGAATTCGACGC+CGG | 0.725090 | 5.1:+64556794 | None:intergenic |
| ACACGCGCTTCTATACCGCG+TGG | 0.787665 | 5.1:-64556660 | MS.gene29628:CDS |
| GCAACAGGGATAACACCACG+CGG | 0.840792 | 5.1:+64556645 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTGTTGATTATGATAA+CGG | - | chr5.1:64556845-64556864 | MS.gene29628:CDS | 20.0% |
| ! | AAAGCGAATACATAAAAACA+TGG | - | chr5.1:64556549-64556568 | MS.gene29628:CDS | 25.0% |
| !!! | TTTTATTGCTGATTCTGATA+AGG | - | chr5.1:64556754-64556773 | MS.gene29628:CDS | 25.0% |
| ! | TTCTAGATCTGTTACTGATT+CGG | - | chr5.1:64556823-64556842 | MS.gene29628:CDS | 30.0% |
| GCTACTTGTCTTACTGTTAA+AGG | - | chr5.1:64556668-64556687 | MS.gene29628:CDS | 35.0% | |
| TGATTCTGATAAGGAGAAAG+AGG | - | chr5.1:64556763-64556782 | MS.gene29628:CDS | 35.0% | |
| !!! | GTTGGTTCGTTTTGAAACAA+GGG | - | chr5.1:64556579-64556598 | MS.gene29628:CDS | 35.0% |
| ACAGTAAGACAAGTAGCAAC+AGG | + | chr5.1:64556665-64556684 | None:intergenic | 40.0% | |
| AGTGGAAAGTTCAAACGAGT+AGG | + | chr5.1:64556717-64556736 | None:intergenic | 40.0% | |
| CAGTAAGACAAGTAGCAACA+GGG | + | chr5.1:64556664-64556683 | None:intergenic | 40.0% | |
| CTGATAAGGAGAAAGAGGAT+TGG | - | chr5.1:64556768-64556787 | MS.gene29628:CDS | 40.0% | |
| GAGGATTGGATTAACTCGAT+TGG | - | chr5.1:64556782-64556801 | MS.gene29628:CDS | 40.0% | |
| GTGGAAAGTTCAAACGAGTA+GGG | + | chr5.1:64556716-64556735 | None:intergenic | 40.0% | |
| TAAAAACATGGCGAAGACGT+TGG | - | chr5.1:64556561-64556580 | MS.gene29628:CDS | 40.0% | |
| TGAAACAAGGGAAACTCTTC+TGG | - | chr5.1:64556591-64556610 | MS.gene29628:CDS | 40.0% | |
| !!! | CGTTGGTTCGTTTTGAAACA+AGG | - | chr5.1:64556578-64556597 | MS.gene29628:CDS | 40.0% |
| !!! | GCTTTGTTTTGTTAACCAGC+CGG | + | chr5.1:64556534-64556553 | None:intergenic | 40.0% |
| ATACATCGTATCAGCGCGAG+TGG | + | chr5.1:64556735-64556754 | None:intergenic | 50.0% | |
| ! | AACCGGTTCAACCACAAACC+CGG | - | chr5.1:64556466-64556485 | MS.gene29628:CDS | 50.0% |
| !! | TTAACCAGCCGGTTCGTTCT+GGG | + | chr5.1:64556523-64556542 | None:intergenic | 50.0% |
| ACAAACCCGGTCGACTACTC+CGG | - | chr5.1:64556479-64556498 | MS.gene29628:CDS | 55.0% | |
| GACCGGGTTTGTGGTTGAAC+CGG | + | chr5.1:64556471-64556490 | None:intergenic | 55.0% | |
| GCAACAGGGATAACACCACG+CGG | + | chr5.1:64556650-64556669 | None:intergenic | 55.0% | |
| GTTCGACCAGAATTCGACGC+CGG | + | chr5.1:64556501-64556520 | None:intergenic | 55.0% | |
| TGGTCGAACCCAGAACGAAC+CGG | - | chr5.1:64556512-64556531 | MS.gene29628:CDS | 55.0% | |
| ! | ACTACTCCGGCGTCGAATTC+TGG | - | chr5.1:64556492-64556511 | MS.gene29628:CDS | 55.0% |
| !! | GTTAACCAGCCGGTTCGTTC+TGG | + | chr5.1:64556524-64556543 | None:intergenic | 55.0% |
| ACACGCGCTTCTATACCGCG+TGG | - | chr5.1:64556632-64556651 | MS.gene29628:CDS | 60.0% | |
| AGTCTGTGGCGCTACGCAAC+CGG | - | chr5.1:64556449-64556468 | MS.gene29628:CDS | 60.0% | |
| GGAGTAGTCGACCGGGTTTG+TGG | + | chr5.1:64556480-64556499 | None:intergenic | 60.0% | |
| CGAACCCAGAACGAACCGGC+TGG | - | chr5.1:64556516-64556535 | MS.gene29628:CDS | 65.0% | |
| TCGACGCCGGAGTAGTCGAC+CGG | + | chr5.1:64556488-64556507 | None:intergenic | 65.0% | |
| CGACGCCGGAGTAGTCGACC+GGG | + | chr5.1:64556487-64556506 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 64556440 | 64556874 | 64556440 | ID=MS.gene29628 |
| chr5.1 | mRNA | 64556440 | 64556874 | 64556440 | ID=MS.gene29628.t1;Parent=MS.gene29628 |
| chr5.1 | exon | 64556440 | 64556874 | 64556440 | ID=MS.gene29628.t1.exon1;Parent=MS.gene29628.t1 |
| chr5.1 | CDS | 64556440 | 64556874 | 64556440 | ID=cds.MS.gene29628.t1;Parent=MS.gene29628.t1 |
| Gene Sequence |
| Protein sequence |
