AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29799.t1 MTR_3g074930 92.370 616 46 1 4 619 8 622 0.0 1210
MS.gene29799.t1 MTR_3g074930 91.332 623 46 2 4 619 8 629 0.0 1204
MS.gene29799.t1 MTR_4g103520 77.600 625 130 3 1 619 6 626 0.0 1040
MS.gene29799.t1 MTR_4g103520 81.193 553 98 2 69 619 1 549 0.0 964
MS.gene29799.t1 MTR_8g063080 68.943 615 184 2 5 619 4 611 0.0 909
MS.gene29799.t1 MTR_5g045600 70.230 608 176 2 14 619 14 618 0.0 908
MS.gene29799.t1 MTR_3g074970 84.713 471 27 2 148 618 100 525 0.0 822
MS.gene29799.t1 MTR_3g074970 95.833 96 4 0 517 612 3 98 1.89e-54 194
MS.gene29799.t1 MTR_3g074960 72.154 492 72 5 4 474 8 455 0.0 706
MS.gene29799.t1 MTR_4g037670 35.640 578 312 18 61 600 79 634 7.76e-107 336
MS.gene29799.t1 MTR_1g052505 35.000 560 309 16 78 600 74 615 4.31e-103 325
MS.gene29799.t1 MTR_4g082930 28.454 608 352 23 42 598 5 580 3.72e-58 207
MS.gene29799.t1 MTR_4g082940 27.632 608 346 23 40 598 4 566 8.88e-52 189
MS.gene29799.t1 MTR_6g005650 82.051 78 14 0 189 266 10 87 2.24e-37 141
MS.gene29799.t1 MTR_1g060360 27.229 415 228 15 189 593 57 407 1.16e-29 122
MS.gene29799.t1 MTR_7g104330 25.908 413 228 15 189 589 65 411 4.89e-29 120
MS.gene29799.t1 MTR_4g115670 26.190 462 277 16 160 602 35 451 9.80e-28 117
MS.gene29799.t1 MTR_7g104360 25.791 411 232 13 189 589 58 405 1.70e-27 115
MS.gene29799.t1 MTR_3g051240 26.218 431 246 14 189 613 54 418 2.83e-26 112
MS.gene29799.t1 MTR_6g013050 27.053 414 186 15 246 589 146 513 1.64e-25 111
MS.gene29799.t1 MTR_6g013050 27.204 397 173 15 246 572 146 496 7.47e-23 103
MS.gene29799.t1 MTR_5g092360 24.390 451 263 18 173 613 42 424 1.46e-22 101
MS.gene29799.t1 MTR_7g056403 25.352 426 202 13 246 600 146 526 2.55e-22 101
MS.gene29799.t1 MTR_3g083810 24.186 430 202 14 246 600 147 527 7.71e-22 100
MS.gene29799.t1 MTR_3g083820 23.776 429 205 14 246 600 147 527 1.68e-20 95.9
MS.gene29799.t1 MTR_0188s0020 24.796 367 223 13 246 595 119 449 4.05e-20 94.4
MS.gene29799.t1 MTR_1g112630 24.122 427 242 19 190 590 70 440 3.53e-18 88.2
MS.gene29799.t1 MTR_4g037105 24.793 363 218 15 246 590 113 438 5.87e-18 87.4
MS.gene29799.t1 MTR_7g405800 93.333 30 2 0 232 261 1 30 6.49e-14 72.0
MS.gene29799.t1 MTR_2g020270 68.085 47 13 1 119 163 33 79 1.01e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29799.t1 AT5G50400 73.159 611 158 3 9 619 6 610 0.0 947
MS.gene29799.t1 AT4G24890 72.287 599 163 2 21 619 19 614 0.0 924
MS.gene29799.t1 AT1G13750 66.667 615 202 1 5 619 1 612 0.0 876
MS.gene29799.t1 AT5G50400 78.824 510 107 1 110 619 30 538 0.0 863
MS.gene29799.t1 AT1G13900 29.568 602 323 26 50 598 24 577 1.37e-58 208
MS.gene29799.t1 AT2G03450 28.642 611 333 25 43 598 15 577 2.16e-57 205
MS.gene29799.t1 AT3G52780 26.218 431 244 15 172 589 36 405 1.85e-28 119
MS.gene29799.t1 AT3G52810 26.450 431 243 17 169 589 42 408 1.39e-27 116
MS.gene29799.t1 AT3G52820 26.728 434 248 18 162 589 35 404 4.12e-24 105
MS.gene29799.t1 AT4G13700 26.970 330 171 11 246 547 140 427 6.59e-24 105
MS.gene29799.t1 AT3G20500 30.466 279 163 10 320 589 147 403 6.67e-24 105
MS.gene29799.t1 AT2G27190 27.671 365 205 15 246 590 118 443 9.42e-23 102
MS.gene29799.t1 AT3G52780 27.507 349 198 12 172 513 36 336 1.52e-22 100
MS.gene29799.t1 AT5G34850 25.203 369 234 12 246 602 112 450 2.02e-22 101
MS.gene29799.t1 AT4G36350 24.044 366 218 13 246 591 114 439 1.35e-19 92.4
MS.gene29799.t1 AT3G07130 24.466 421 209 15 246 600 140 517 2.48e-19 92.0
MS.gene29799.t1 AT4G13700 26.804 291 153 10 246 508 140 398 5.69e-19 90.1
MS.gene29799.t1 AT1G56360 22.782 417 264 16 191 591 65 439 1.01e-18 89.7
MS.gene29799.t1 AT2G18130 25.714 280 179 10 338 602 168 433 4.45e-15 78.6
MS.gene29799.t1 AT2G32770 22.170 424 201 16 246 589 149 523 6.92e-15 78.2
MS.gene29799.t1 AT3G07130 22.676 441 225 16 246 600 140 550 1.07e-14 77.8
MS.gene29799.t1 AT2G32770 23.343 347 172 14 246 547 68 365 8.28e-13 71.2

Find 167 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTTCATTTACCTTTAT+TGG 0.162930 3.4:-73363819 None:intergenic
TTTAATGCACACCTCATATT+TGG 0.186772 3.4:-73364983 None:intergenic
TAGTAGTTATACCTGATAAA+TGG 0.220072 3.4:-73366776 None:intergenic
GAGTTTCATTTACCTTTATT+GGG 0.220701 3.4:-73363818 None:intergenic
CAGTCTTCATTAGTTGGTTC+TGG 0.230728 3.4:-73362373 None:intergenic
TACCTTACTGCCAAGCAAAA+AGG 0.234836 3.4:-73360318 None:intergenic
TAATATAACTCACCAGAATT+TGG 0.238636 3.4:-73365783 None:intergenic
TGAATCTGCTTTCCACCCTT+TGG 0.258361 3.4:-73364748 None:intergenic
TGCAGGTATGCCACAGATTA+TGG 0.265751 3.4:+73366472 MS.gene29799:intron
GAATCTGCTTTCCACCCTTT+GGG 0.297542 3.4:-73364747 None:intergenic
GCGAACCTTGCTATCATATA+TGG 0.299475 3.4:-73365553 None:intergenic
GGGAAAGTTTGCAGGGGCTT+TGG 0.317516 3.4:+73366692 MS.gene29799:CDS
AGAGACAAAGACTATGGTTT+TGG 0.320936 3.4:+73367040 MS.gene29799:CDS
CATGGGAAGGGAAAGTTTGC+AGG 0.323081 3.4:+73366684 MS.gene29799:CDS
TTCTATGACACTCCAGATTC+AGG 0.324023 3.4:+73365704 MS.gene29799:CDS
CGCGGATACACAGGTGCTTT+AGG 0.325055 3.4:-73364474 None:intergenic
CTGCACGTTGATTGATCAAT+TGG 0.331585 3.4:-73363995 None:intergenic
CCGCATTCTCCACCTGAATC+TGG 0.338489 3.4:-73365716 None:intergenic
TTGGGTGATATGAATGGATT+TGG 0.345669 3.4:+73360218 MS.gene29799:CDS
AAGAGTATGCCCCATTTATC+AGG 0.358857 3.4:+73366765 MS.gene29799:CDS
TTGCAACGTGTTATCATATT+CGG 0.359394 3.4:+73365183 MS.gene29799:CDS
CCCAGGTACGCCTATAGGTT+GGG 0.360573 3.4:+73365081 MS.gene29799:intron
CACCAACTTGAGGATCTGTT+TGG 0.360863 3.4:+73360199 MS.gene29799:CDS
AGTAGTTATACCTGATAAAT+GGG 0.363474 3.4:-73366775 None:intergenic
AGTCTTCATTAGTTGGTTCT+GGG 0.366107 3.4:-73362372 None:intergenic
CGTCCAATGCCACCGGGGTT+GGG 0.366293 3.4:-73363768 None:intergenic
ACCGTTGGCTGGCGCGATCC+TGG 0.370934 3.4:+73364926 MS.gene29799:CDS
GGCATACTCTTTCGTAGTTA+TGG 0.374674 3.4:-73366753 None:intergenic
CTGCAAACTTTCCCTTCCCA+TGG 0.377219 3.4:-73366683 None:intergenic
CATTATTCTGGCACTGTGAA+TGG 0.377604 3.4:+73366938 MS.gene29799:CDS
TCACCACAGCACCTCCTGTT+TGG 0.380045 3.4:+73367008 MS.gene29799:CDS
AGCTGTACCATTTGTTGAAT+GGG 0.384608 3.4:+73364723 MS.gene29799:CDS
GTATGTTACTTGAAGTTTGC+AGG 0.390132 3.4:-73362412 None:intergenic
CAGGTTTCCTTGAAGAACTT+TGG 0.392296 3.4:+73364960 MS.gene29799:CDS
CCGTCCAATGCCACCGGGGT+TGG 0.396303 3.4:-73363769 None:intergenic
AGGTAAGTTAAACATGAGTT+TGG 0.397660 3.4:+73360157 MS.gene29799:CDS
CTGAGAACAAAGCCAAATTC+TGG 0.397715 3.4:+73365771 MS.gene29799:CDS
AGAATGGGTGACAGTAAAAC+TGG 0.399637 3.4:+73362345 MS.gene29799:CDS
CATGTTGTTGTTGGTGGAGG+GGG 0.399824 3.4:+73366968 MS.gene29799:CDS
TGTCTGATGGCTCTTATGTA+TGG 0.401100 3.4:+73365112 MS.gene29799:CDS
CATATGCTCAGCCCCAATAA+AGG 0.402084 3.4:+73363806 MS.gene29799:CDS
TCATAGAAGGATCCTGAGTT+TGG 0.403373 3.4:-73365689 None:intergenic
CAATAAATGCCCCAACCTAT+AGG 0.409594 3.4:-73365091 None:intergenic
TCTTCCCCAGGTACGCCTAT+AGG 0.412213 3.4:+73365076 MS.gene29799:intron
CCAACCCAGTCTTCATTAGT+TGG 0.419485 3.4:-73362379 None:intergenic
ATCTCTATAGTCCCTGGAAA+TGG 0.436173 3.4:-73367143 None:intergenic
TTCAAGGCATCTCCTTATCC+TGG 0.438321 3.4:+73365150 MS.gene29799:CDS
AAGAACTTTGGCCAAATATG+AGG 0.438770 3.4:+73364972 MS.gene29799:CDS
AATTTGGCTTTGTTCTCAGC+AGG 0.439009 3.4:-73365767 None:intergenic
CTTGGCATACCAATCATTAG+AGG 0.440011 3.4:-73366641 None:intergenic
CCACCCAACCCCGGTGGCAT+TGG 0.440027 3.4:+73363765 MS.gene29799:CDS
AACCTGCGCGAACCGTTGGC+TGG 0.449374 3.4:+73364915 MS.gene29799:CDS
TGAATCTGGAGTGTCATAGA+AGG 0.450964 3.4:-73365702 None:intergenic
CTCTTCAAATGAACCCTCCT+TGG 0.453099 3.4:-73366659 None:intergenic
AAGCAGATTCAATCTGCTGC+TGG 0.454182 3.4:+73364760 MS.gene29799:CDS
TTTGGGCCCCATTCAACAAA+TGG 0.461006 3.4:-73364730 None:intergenic
ACCAACTTGAGGATCTGTTT+GGG 0.461280 3.4:+73360200 MS.gene29799:CDS
TATTTGGCCAAAGTTCTTCA+AGG 0.461537 3.4:-73364967 None:intergenic
GGAACAATTCATGTTGTTGT+TGG 0.462326 3.4:+73366959 MS.gene29799:CDS
GTGGAAATCACGAACGCTCT+TGG 0.464456 3.4:+73365666 MS.gene29799:intron
CTCAGCAGGGTAATAGTACA+TGG 0.467918 3.4:-73365753 None:intergenic
ATCTGTTTGGGTGATATGAA+TGG 0.471658 3.4:+73360212 MS.gene29799:CDS
ATTCATGTTGTTGTTGGTGG+AGG 0.474320 3.4:+73366965 MS.gene29799:CDS
TTGGGGCATTTATTGTCTGA+TGG 0.475270 3.4:+73365099 MS.gene29799:CDS
CCCCAGGTACGCCTATAGGT+TGG 0.480019 3.4:+73365080 MS.gene29799:intron
TCTCTGAAAATACTCCAAAC+AGG 0.481381 3.4:-73367022 None:intergenic
CAGGGACTATAGAGATGTCT+TGG 0.482107 3.4:+73367150 MS.gene29799:CDS
GTATTTAGGATTGGAGTGGT+TGG 0.482977 3.4:-73363943 None:intergenic
GTGTATCCGCGCCTTGCTCA+TGG 0.485506 3.4:+73364486 MS.gene29799:CDS
AAGCTGTACCATTTGTTGAA+TGG 0.486572 3.4:+73364722 MS.gene29799:CDS
TTCATGTTGTTGTTGGTGGA+GGG 0.486891 3.4:+73366966 MS.gene29799:CDS
TTTGAAACTGCAACAAGCTT+AGG 0.488913 3.4:-73364432 None:intergenic
TCGGTTACTCCTCTAATGAT+TGG 0.490311 3.4:+73366632 MS.gene29799:CDS
TGTCTAAATCCTTGATGAGC+TGG 0.492936 3.4:-73365426 None:intergenic
ATCTACCCGTCCAATGCCAC+CGG 0.493173 3.4:-73363775 None:intergenic
GCTCTTATGTATGGAGTAAG+AGG 0.494084 3.4:+73365121 MS.gene29799:CDS
ACAATTCATGTTGTTGTTGG+TGG 0.495571 3.4:+73366962 MS.gene29799:CDS
CAGAACCAACTAATGAAGAC+TGG 0.498767 3.4:+73362374 MS.gene29799:CDS
CGCCAGCCAACGGTTCGCGC+AGG 0.499949 3.4:-73364917 None:intergenic
TGCAAACTTTCCCTTCCCAT+GGG 0.500947 3.4:-73366682 None:intergenic
ATTGGGGCTGAGCATATGAA+TGG 0.502132 3.4:-73363801 None:intergenic
AGAATCATGGATCGCAAGAA+TGG 0.504874 3.4:-73360276 None:intergenic
GGAGATCTGCCATATGCAAA+TGG 0.505321 3.4:+73365472 MS.gene29799:CDS
TTTGTTGAATGGGGCCCAAA+GGG 0.507205 3.4:+73364733 MS.gene29799:CDS
AGTTTCATTTACCTTTATTG+GGG 0.515764 3.4:-73363817 None:intergenic
CCAACCCCGGTGGCATTGGA+CGG 0.516793 3.4:+73363769 MS.gene29799:CDS
GAGTAAGAGGTATTCATTCA+AGG 0.520098 3.4:+73365134 MS.gene29799:CDS
GGTCAACCTGCGCGAACCGT+TGG 0.521129 3.4:+73364911 MS.gene29799:intron
CAACCCCGGTGGCATTGGAC+GGG 0.532592 3.4:+73363770 MS.gene29799:CDS
TGAGCAAGGCGCGGATACAC+AGG 0.538957 3.4:-73364483 None:intergenic
GAACGCTCTTGGCCAAACTC+AGG 0.539098 3.4:+73365677 MS.gene29799:CDS
CAGTAACGGAGGCAGAATCA+TGG 0.541508 3.4:-73360289 None:intergenic
TAATAGTACATGGTTTCTGC+AGG 0.541874 3.4:-73365743 None:intergenic
CAAGAAAAGTAGTGATGGAA+AGG 0.542299 3.4:+73367105 MS.gene29799:CDS
GTCCTTTAGGCTGAGCGTGA+TGG 0.547365 3.4:+73365355 MS.gene29799:intron
GTCTTCATTAGTTGGTTCTG+GGG 0.549003 3.4:-73362371 None:intergenic
TCCAAGACTTTCCATGAGCA+AGG 0.549311 3.4:-73364497 None:intergenic
TCATGTTGTTGTTGGTGGAG+GGG 0.552640 3.4:+73366967 MS.gene29799:CDS
TATGACAGATGACAGTTACC+TGG 0.552868 3.4:+73364677 MS.gene29799:intron
TGTTATCATATTCGGTGACA+TGG 0.552892 3.4:+73365191 MS.gene29799:CDS
CCAATGCCACCGGGGTTGGG+TGG 0.553233 3.4:-73363765 None:intergenic
GGGTTCATTTGAAGAGCCCA+TGG 0.554005 3.4:+73366666 MS.gene29799:CDS
CTCTAATGATTGGTATGCCA+AGG 0.554950 3.4:+73366642 MS.gene29799:CDS
TCTACCCGTCCAATGCCACC+GGG 0.555257 3.4:-73363774 None:intergenic
CCAGGTACGCCTATAGGTTG+GGG 0.555647 3.4:+73365082 MS.gene29799:intron
ATTTGTTGAATGGGGCCCAA+AGG 0.559998 3.4:+73364732 MS.gene29799:CDS
TAGCAGACACTGAACATGAC+TGG 0.560714 3.4:+73366512 MS.gene29799:CDS
GCAGGGCGAGGATACAGAAT+GGG 0.561327 3.4:+73362330 MS.gene29799:intron
TATGTCATATCCACTTGTCC+AGG 0.562397 3.4:-73364695 None:intergenic
GCCTCTTCATCACCAACTTG+AGG 0.562952 3.4:+73360189 MS.gene29799:CDS
TCCTCAAGTTGGTGATGAAG+AGG 0.564410 3.4:-73360190 None:intergenic
ATTTGAAGAGCCCATGGGAA+GGG 0.565074 3.4:+73366672 MS.gene29799:CDS
GAGATGTATCCATTTGCATA+TGG 0.566529 3.4:-73365481 None:intergenic
CAAGACATCTCTATAGTCCC+TGG 0.567071 3.4:-73367149 None:intergenic
GAATATGCTAATTATCAACC+TGG 0.571971 3.4:+73365385 MS.gene29799:CDS
ACTGAACATGACTGGAGAGA+AGG 0.580541 3.4:+73366520 MS.gene29799:CDS
CACCACTGACCAGCTCATCA+AGG 0.584625 3.4:+73365417 MS.gene29799:CDS
ACCAGGATCGCGCCAGCCAA+CGG 0.585321 3.4:-73364927 None:intergenic
GAGTACAAGAAAAGTAGTGA+TGG 0.585752 3.4:+73367100 MS.gene29799:CDS
TGCAGGGCGAGGATACAGAA+TGG 0.587032 3.4:+73362329 MS.gene29799:intron
CATATTCGGTGACATGGGAA+AGG 0.587852 3.4:+73365197 MS.gene29799:CDS
ATGACAGTTACCTGGACAAG+TGG 0.589057 3.4:+73364685 MS.gene29799:CDS
ACCCAAACAGATCCTCAAGT+TGG 0.593043 3.4:-73360201 None:intergenic
CAGTTCCATATATGATAGCA+AGG 0.595584 3.4:+73365548 MS.gene29799:CDS
ATGGGAAGGGAAAGTTTGCA+GGG 0.596275 3.4:+73366685 MS.gene29799:CDS
CCAACTAATGAAGACTGGGT+TGG 0.597652 3.4:+73362379 MS.gene29799:CDS
TCCACCTGAAAATAGTGCAA+AGG 0.604263 3.4:-73364024 None:intergenic
GTTGAATGGGGCCCAAAGGG+TGG 0.605971 3.4:+73364736 MS.gene29799:CDS
TTTGCAGCACATCGCCCACT+CGG 0.606514 3.4:+73366613 MS.gene29799:CDS
TGAATGGCAATCTTTGAAAG+TGG 0.607314 3.4:-73360248 None:intergenic
CATTTGAAGAGCCCATGGGA+AGG 0.608262 3.4:+73366671 MS.gene29799:CDS
CATATGGCAGATCTCCTATG+TGG 0.608314 3.4:-73365465 None:intergenic
GCCTTGCTCATGGAAAGTCT+TGG 0.610045 3.4:+73364496 MS.gene29799:CDS
AAACCTGTGTGTATAAAACC+AGG 0.612380 3.4:-73364944 None:intergenic
GTTATCATATTCGGTGACAT+GGG 0.619175 3.4:+73365192 MS.gene29799:CDS
ACATGACTGGAGAGAAGGAT+CGG 0.620114 3.4:+73366525 MS.gene29799:CDS
CCAGATTCAGGTGGAGAATG+CGG 0.625344 3.4:+73365716 MS.gene29799:CDS
AATGATTGGTATGCCAAGGA+GGG 0.625443 3.4:+73366646 MS.gene29799:CDS
ATTTGGCTTTGTTCTCAGCA+GGG 0.626371 3.4:-73365766 None:intergenic
CTCACAACCATCGTGCACAC+AGG 0.626678 3.4:-73367173 None:intergenic
ACTCCAAACAGGAGGTGCTG+TGG 0.627083 3.4:-73367011 None:intergenic
TAATGATTGGTATGCCAAGG+AGG 0.627561 3.4:+73366645 MS.gene29799:CDS
AGAACCAACTAATGAAGACT+GGG 0.631293 3.4:+73362375 MS.gene29799:CDS
ATCCTTGATGAGCTGGTCAG+TGG 0.633150 3.4:-73365419 None:intergenic
GGTTCATTTGAAGAGCCCAT+GGG 0.633987 3.4:+73366667 MS.gene29799:CDS
GACTTTCCATGAGCAAGGCG+CGG 0.635264 3.4:-73364492 None:intergenic
TCAGTGGTGTTAAGTGAACC+AGG 0.637131 3.4:-73365403 None:intergenic
TTTAATCGTAACAGCATGTG+TGG 0.639129 3.4:+73364793 MS.gene29799:CDS
TCAACATGTCCACCCAACCC+CGG 0.639236 3.4:+73363756 MS.gene29799:CDS
TATGACACTCCAGATTCAGG+TGG 0.641435 3.4:+73365707 MS.gene29799:CDS
CATAATTCAGAGTGCCAAGG+TGG 0.647479 3.4:-73367200 None:intergenic
GCTGTACCATTTGTTGAATG+GGG 0.649227 3.4:+73364724 MS.gene29799:CDS
ACGAAACATGCCATAATCTG+TGG 0.650821 3.4:-73366482 None:intergenic
TAGATAGAAAACAACAACCA+TGG 0.654457 3.4:+73366584 MS.gene29799:CDS
AAATGGATACATCTCACAGT+GGG 0.656289 3.4:+73365489 MS.gene29799:CDS
CTACATAATTCAGAGTGCCA+AGG 0.658085 3.4:-73367203 None:intergenic
GTCTTGGCCTGTGTGCACGA+TGG 0.663436 3.4:+73367166 MS.gene29799:CDS
GTAGTTATACCTGATAAATG+GGG 0.675933 3.4:-73366774 None:intergenic
CATTAGAGGAGTAACCGAGT+GGG 0.692825 3.4:-73366627 None:intergenic
CTGAAAATACTCCAAACAGG+AGG 0.700173 3.4:-73367019 None:intergenic
TGGGAAGGGAAAGTTTGCAG+GGG 0.702638 3.4:+73366686 MS.gene29799:CDS
TCATTAGAGGAGTAACCGAG+TGG 0.714971 3.4:-73366628 None:intergenic
TGGTTGTGAGAAAACCACCT+TGG 0.719747 3.4:+73367186 MS.gene29799:CDS
ACATGTCCACCCAACCCCGG+TGG 0.724016 3.4:+73363759 MS.gene29799:CDS
CTACCCGTCCAATGCCACCG+GGG 0.725369 3.4:-73363773 None:intergenic
CAAATGGATACATCTCACAG+TGG 0.732996 3.4:+73365488 MS.gene29799:CDS
TGTGCTGCAAAGATTAACCA+TGG 0.735965 3.4:-73366601 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.4 gene 73360152 73367216 73360152 ID=MS.gene29799
chr3.4 mRNA 73360152 73367216 73360152 ID=MS.gene29799.t1;Parent=MS.gene29799
chr3.4 exon 73360152 73360337 73360152 ID=MS.gene29799.t1.exon1;Parent=MS.gene29799.t1
chr3.4 CDS 73360152 73360337 73360152 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73362334 73362425 73362334 ID=MS.gene29799.t1.exon2;Parent=MS.gene29799.t1
chr3.4 CDS 73362334 73362425 73362334 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73363752 73363827 73363752 ID=MS.gene29799.t1.exon3;Parent=MS.gene29799.t1
chr3.4 CDS 73363752 73363827 73363752 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73363933 73364055 73363933 ID=MS.gene29799.t1.exon4;Parent=MS.gene29799.t1
chr3.4 CDS 73363933 73364055 73363933 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73364432 73364524 73364432 ID=MS.gene29799.t1.exon5;Parent=MS.gene29799.t1
chr3.4 CDS 73364432 73364524 73364432 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73364685 73364814 73364685 ID=MS.gene29799.t1.exon6;Parent=MS.gene29799.t1
chr3.4 CDS 73364685 73364814 73364685 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73364912 73364993 73364912 ID=MS.gene29799.t1.exon7;Parent=MS.gene29799.t1
chr3.4 CDS 73364912 73364993 73364912 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73365086 73365218 73365086 ID=MS.gene29799.t1.exon8;Parent=MS.gene29799.t1
chr3.4 CDS 73365086 73365218 73365086 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73365364 73365569 73365364 ID=MS.gene29799.t1.exon9;Parent=MS.gene29799.t1
chr3.4 CDS 73365364 73365569 73365364 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73365667 73365792 73365667 ID=MS.gene29799.t1.exon10;Parent=MS.gene29799.t1
chr3.4 CDS 73365667 73365792 73365667 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73366477 73366786 73366477 ID=MS.gene29799.t1.exon11;Parent=MS.gene29799.t1
chr3.4 CDS 73366477 73366786 73366477 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
chr3.4 exon 73366911 73367216 73366911 ID=MS.gene29799.t1.exon12;Parent=MS.gene29799.t1
chr3.4 CDS 73366911 73367216 73366911 ID=cds.MS.gene29799.t1;Parent=MS.gene29799.t1
Gene Sequence

>MS.gene29799

ATGGAAGGTAAGTTAAACATGAGTTTGGTAATATTGTGCCTCTTCATCACCAACTTGAGGATCTGTTTGGGTGATATGAATGGATTTGGAGAGCAGCCACTTTCAAAGATTGCCATTCACAAAACCATTCTTGCGATCCATGATTCTGCCTCCGTTACTGCTTCCCCCTTTTTGCTTGGCAGTAAGGTAGTATCTTCTACCCCGTTTCATTAATTTAACCTATTTATTTTGTTCTCACTAGCATGACATTAACATGCATGTTTTTCCACTTTTATTAGCAATGAATGATAATATGTTACAAAACAATTAAGTGAAATGTTAAATTGGAAGAAAAAATGTTACACCAAAATAGTTAAGTAATTCATTAATAGAAATTTTGTCTTGTAAATATAAGCTTTTACTTTTGTCTTAGTAATAATCACACCATTTTTTGATACAAACTTACTAATTTTAACACTTTAACCAATATCTCAAGGGCATTCATTAGCATTCTCCTTCCTTTATATATTGATATAGATTTATCAACAAATGTTTTGATTAAAAAAAATAACTATATTATTTTTTGAGAGAACTAATGAAATTTAGTTTTTTGAGGGGAGAATTAATGAAAGTTAAATAGTACTTGTTTCCCTCAACAAAACAAAACAGAAAATAGTACTTGATCAAGACATTTTATTTCTGCACAACTGCACCCCTTGATTTTACTTTCTACACCTGTTTGAGGCGATTTGAGCTTTGGTATTTTTCTCCAGCCAGAGAAAAACGATACAATTACTGTAATTTTAGCTAGTACTAGCATGAAAATAGTTTTTTTTTTTTAGAGAAGCATGAAAATAGTTTAAAAGATGCAAAATTGTTTGCATGTAGCTCATAAATCTTTTATATGTTCGGTTTTCGTTGATTAGTGTCACATTTATTAAAATAACTATAATTTAAAATAAATCTTGTTTTTAATATTTTATAGGCTTAAATGCGCTTTTGGTCCCTTAACTATTTATTTGATATCGCTTTGGTCCCTTAACTAAAAAAAAGATTGTTTTGGTATTTTATCTTTATCTCCGTTACCTGTTTTGGTCCCTTCCGTTAAATAAATTCAAAAAAACGTTAGGGTTTGTACTATTCATCTTCTTCCTTCTCCTTCCATCCTGTTCTTCATCATCAACAACACACCAACCCATATTTTGGGACCAAAACGATACCAAATAAATATTAAGGGATCAAAAGAACATTTAAGTTTTTTTTTTTTTATCATCTTCTTCATCTTCATCAAAACTCATATTCATCAATTAAATCCAGAAAAAAAAAAAAAAAACACAGAATGCTCATATTTGGAAGAGTTATGCATAATGCTCAAATCAAAACTCATATTTGGCTTAAACTCATCAAAATAAAATAAAAAACACATAATGCTCAAATCAAAACTCAAATTTGGCTTAAACTCATATCAAAATCAAAACTTCAAAAGAGTTATGCATAATGCTCAAATTTGAAAAAACCTCCTTTAGAAAAACCCATCCTTCAAAAATTCGAAACAGGAGAAGAGTAAGGTTCTGCAGAATGAATTTGAAACAGGAAAAGAGTAAGATTATGCAGAATGAATTCGAAATGGGTGAAAATTAAGGTTCAAAAATTTGAGTTTTGCGAGAAGAGATTGAGGTTGTAAACAGGAGAAGATTTGAGTTTGAGATTCAACAAAGGATTACTTAAAGAAGGAGGTTTAAGTTTGAGATTCAACACAGGACACATAAGAGGAAAAGATTGAAGGATATGCTATTTTTTAGTTTTTTTTTGGATTATTTTTTGTGTTTTTTTTCTGGATTTAATTGATGAATATGAGTTTTGATGAAGATGAAGAAGAAAAACTTAAATGTTCTTTTGGTCCCTTAATTATTTATTTGGTATCGTTTTGGTCCCAAAATCTGGGTTTAGTTGATGAATGATGAAGGTCTGGGTTTAGTTGATGAATGATGAAGGTTTTGAGAAGGAAGAAGATGAATAGTACAAACCCTAACGTTTTTTTGAATTTATTTAACGGAAAGGACCAAAACAGGTAACAGAGATAAAGATAAAATACTAAAACAATCTTTTTTTTAGTTAAGGGACCAAAGCGATACCAAATAAATAGTTAAGGGACCAAAAGCGCATTTAAGCCTATTTTATAGTTGTACTCATACTATTGCAGGGCGAGGATACAGAATGGGTGACAGTAAAACTGGAGTCCCCAGAACCAACTAATGAAGACTGGGTTGGAGTTTTTACTCCTGCAAACTTCAAGTAACATACAACAATTCTCTCTCTCTCTCTCTCACTTTTAATATTGTTCTTTAAACACATTTTTCAAAAACATCATATCATACATATACTAAGACACTTGAGTGAATTTTGTATGAATCATATTGGTACATATTCACAATGTTAACACAGTCATGAAAAAGTACATTGACAATAGGAGTTTTTTGATTCCTCTTATGTTTTCTCTCTCTGTTTTATAATTGGAATAAAAAAAAAGAACTTGTAGTAAGTTTGCTCTCTAAAATTCAGCTCCATAACATTGCATGGTAGCCATGTGGAAAGATATGCCTTGGACTAACACAGAAGGAGTTAGTCACATGATCACACAACCTTATCTACACTTAGTCACATGAAACACCTTGGATGTAAGCAACATTCTGAATATGTCTAAAACAAAACCAACGTGAGTTAACTTACGGTGTTTGGAACGTTACTTAAGTTACGTAGTTTTTATTTCAATGTGATTTAAGTCATGTTGGGTTTAATATCTGCGCGACTTAAGTCACGTGGAGAGTATTTTAGGAAGTTTGATTGGGAGCAAGCAAATCTTGATGGGAGAAGAGCAATTTTCTTTATTTTACTTAATGACCCCTTAATTTTATGTTTGGGGTCCAAATTTTGAACTTATTTATTGGGACTGACGGTCGTCCTCCTCATCCTGCCTCAGATACGGCCCTTGTCATACCTATGTCTAGTAAACCTTATGTCATGTTCCTTAAATCCAATTTATCCAATGAGAGTTTCTTTTTTCACTCGCACGATTCTTAAGAACCGTTCGCATCTAAAAAAGGGATGGGAGGGGAGAGTCTTGAAGGAAAAAAAAATGAAGAATGAAGTGGATATAACAAAATATTTTGAAAGGGGAGGGTTTAGGAGTTTATTTTTCTTCATGGTACAAAATTCTTCCAACTTAAAACTTGTTTTTGTCGACAAATTCTAATTTAGAATTTAAAAACTTGTATTGAAGGATGATTTTGGAGGATTTTAGAAGATTAGGATAAATTTTCCAAATTTAATTTTTTTTTTTAAGGAAGGATAAATTTTCCAAATTTAATATATATTGTTATAATATTTTCAATATTAAAAATATATTAATCATAAGTATTTATTTATCATTTTCTACAAAACATTTCAAAAAAATAATCAATTCTCTAGTCTTTTCGCTACTCCCTCCTTCCCGAAGTTCTCCCATCCTTTTTCCTCCAAACTCTCCAACATCGCCTAAAGAGTCATGGCCATAAACTATGCATATATCTCACTATTATGGCTGAAAAACGAACTAAAGGAAATATATTATTGAAATCACAGTTCGTCAACATGTCCACCCAACCCCGGTGGCATTGGACGGGTAGATACTCCATTCATATGCTCAGCCCCAATAAAGGTAAATGAAACTCCATCTCATATAAATTGTGTATGAAATGAATGAACAACATAACAAGGATGAACCTCTCATGTGTGTTGATTATAATTTGAATCTTTGTAATAGTACAAGTATGCCAACCACTCCAATCCTAAATACAAAAAGACTGGGAAAACTACTTTGAAGTTCCAATTGATCAATCAACGTGCAGATTTCTCCTTTGCACTATTTTCAGGTGGATTATCCAATGTAAGTCATATCTACTGATCTTTTTTTTTTTTTTTTACTATCTTTAAATTTTTTTGTCTTTGCTTAGATGTAGATTTCACATTTAAAAAATCAGTGTCATATACTAGTGCTATTAGAGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTGAAAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAACAGGGTAAGGCTGTGTACAATACACCAAATGGTGGGACCCCTTCCCAGACCCTGCATATGCGGGAGCTCTAGTGCACCGGGTTGCCCCTTTATATACTAGTGCTATTAGTATGCAATGATCCTTATTCAATTGTATGCTAACAAGTGTTCATTTGATTGAGCATTGCAGCCTAAGCTTGTTGCAGTTTCAAACTTCATTGCATTTGCAAATCCTAAAGCACCTGTGTATCCGCGCCTTGCTCATGGAAAGTCTTGGAATGAAGTAAGTTTATCTAGTCAATTTAATTGCATACTAGTTGCCTAACATTTCCTAATTATACATTGGTTCTTATCATCTAAAACTTTTGTAATTGAAATTGCTGCATGAATCGTCTAACTTAAACGTTTAGAATAATGATAAGAAATTTGTTCTTCTATGACAGATGACAGTTACCTGGACAAGTGGATATGACATAAGTGAAGCTGTACCATTTGTTGAATGGGGCCCAAAGGGTGGAAAGCAGATTCAATCTGCTGCTGGAACATTGACATTTAATCGTAACAGCATGTGTGGTATGGATTAATACATCGTAACTCAATTTCATACCTTATTATCTTTTTCTAAAAATGATTCTCAACATATAATGAGGGACATTTTGTTAATCTGTAGGTCAACCTGCGCGAACCGTTGGCTGGCGCGATCCTGGTTTTATACACACAGGTTTCCTTGAAGAACTTTGGCCAAATATGAGGTGTGCATTAAACTATATTTATGTTTATGAGACTATAATTGATAAGTAGAGTTCTGAAAGCTTAGCTACCTTCCCTCCCTATTCTTCCCCAGGTACGCCTATAGGTTGGGGCATTTATTGTCTGATGGCTCTTATGTATGGAGTAAGAGGTATTCATTCAAGGCATCTCCTTATCCTGGACAAAACTCATTGCAACGTGTTATCATATTCGGTGACATGGGAAAGGTAGCGTCGATTATTTAATGTTTATAACATTGGAAAGATTGATCACCTAACTAGATAATTTTCCAACTTTTTATCTAAAAACTCTAATTTGAAACTTGATTTGTACAATGGGGAGAACACTTATTTTGGTTCACATGTCCTTTAGGCTGAGCGTGATGGTTCAAATGAATATGCTAATTATCAACCTGGTTCACTTAACACCACTGACCAGCTCATCAAGGATTTAGACAATATTGACATTGTTTTCCACATAGGAGATCTGCCATATGCAAATGGATACATCTCACAGTGGGATCAATTCACAGCTCAAGTACAACAAATTTCATCAACAGTTCCATATATGATAGCAAGGTTCGCTTGCTTGCAATCATTTTAGGCCATAGAAATTTGAATTGAGTTTTACATATTCTTTTAGACTGAAAGGATATCACATTTATTCATACTGCAGTGGAAATCACGAACGCTCTTGGCCAAACTCAGGATCCTTCTATGACACTCCAGATTCAGGTGGAGAATGCGGAGTTCCTGCAGAAACCATGTACTATTACCCTGCTGAGAACAAAGCCAAATTCTGGTGAGTTATATTAATCTAAATGTAAACCATGACATGTTTAGCATTAATAAAACAGATTTTCTTTTCTCAACTGAATTTTTCACTACTTGGTCCAAGATAGTACGTATGATCCTGACCAATGGTTGCGTAATTATTTCTATAATTTCATTGTTGGAACAAAGTATGGTTTTGTGTTGATTTTTGAAAGAATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGATTGAGATTAAGAGATTAAGAGTTTGAGAATAATGAGAGGAGTAATATTGCTTTCTCAAATTCAGTTACAATATGCATACAACATAGAAGCTATTTATAGGCATGGTTTTGTTACAATAGATTGCTTAACACGCAAGCAATGCATATAACAAACCCTAACTGTTTTATTTGCAAGCTAAACAAACTTAAAACTAACTTCTAACAAACTTAACAAACTGAATTAACACATTCCAGCATTCATGATATCTGCAAAAGATACATTTTCATCCTTATAGTTCATGGAATCATGGTGCAGTTTCTAGTTTTTGCAAGTGAAAAATAAACTAGGAGGTACTATTTTTCCATTCCTAATTAATGACGAAAATTGGAAATATGCAGGTATGCCACAGATTATGGCATGTTTCGTTTCTGTATAGCAGACACTGAACATGACTGGAGAGAAGGATCGGAACAGTACAAATTCATCGAGCATTGCCTTGCAACAGTAGATAGAAAACAACAACCATGGTTAATCTTTGCAGCACATCGCCCACTCGGTTACTCCTCTAATGATTGGTATGCCAAGGAGGGTTCATTTGAAGAGCCCATGGGAAGGGAAAGTTTGCAGGGGCTTTGGCAAAAGTATAAAGTAGACATTGCATTTTATGGTCACGTCCATAACTACGAAAGAGTATGCCCCATTTATCAGGTATAACTACTATTGTCACATTTCAGTTAAAGCGAGATGTGCTGTCTATATTAAGTACTTGATTGTTACAGTTTTTACCGACAGCCTACATCCATTTCTAATTATCCAAATGTGTATAATGCAGAACCAATGCGTGAATAAAGAAAAATCTCATTATTCTGGCACTGTGAATGGAACAATTCATGTTGTTGTTGGTGGAGGGGGAAGTCACTTGTCAGACTTCACCACAGCACCTCCTGTTTGGAGTATTTTCAGAGACAAAGACTATGGTTTTGGCAAACTGACAGCATTTAATCACTCATATCTCTTGTTTGAGTACAAGAAAAGTAGTGATGGAAAGGTCTATGATTCTTTTACCATTTCCAGGGACTATAGAGATGTCTTGGCCTGTGTGCACGATGGTTGTGAGAAAACCACCTTGGCACTCTGA
Protein sequence

>MS.gene29799.t1

MEGKLNMSLVILCLFITNLRICLGDMNGFGEQPLSKIAIHKTILAIHDSASVTASPFLLGSKGEDTEWVTVKLESPEPTNEDWVGVFTPANFNSSTCPPNPGGIGRVDTPFICSAPIKYKYANHSNPKYKKTGKTTLKFQLINQRADFSFALFSGGLSNPKLVAVSNFIAFANPKAPVYPRLAHGKSWNEMTVTWTSGYDISEAVPFVEWGPKGGKQIQSAAGTLTFNRNSMCGQPARTVGWRDPGFIHTGFLEELWPNMRYAYRLGHLLSDGSYVWSKRYSFKASPYPGQNSLQRVIIFGDMGKAERDGSNEYANYQPGSLNTTDQLIKDLDNIDIVFHIGDLPYANGYISQWDQFTAQVQQISSTVPYMIASGNHERSWPNSGSFYDTPDSGGECGVPAETMYYYPAENKAKFWYATDYGMFRFCIADTEHDWREGSEQYKFIEHCLATVDRKQQPWLIFAAHRPLGYSSNDWYAKEGSFEEPMGRESLQGLWQKYKVDIAFYGHVHNYERVCPIYQNQCVNKEKSHYSGTVNGTIHVVVGGGGSHLSDFTTAPPVWSIFRDKDYGFGKLTAFNHSYLLFEYKKSSDGKVYDSFTISRDYRDVLACVHDGCEKTTLAL