Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30310.t1 | XP_027337328.1 | 45.2 | 241 | 93 | 6 | 1 | 213 | 5 | 234 | 4.50E-47 | 198 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30310.t1 | A0A2K3LF81 | 39.8 | 244 | 92 | 6 | 8 | 199 | 2 | 242 | 1.2e-36 | 162.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051588 | MS.gene30310 | 0.815881 | 7.37E-52 | -1.69E-46 |
| MS.gene054102 | MS.gene30310 | 0.807352 | 5.27E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene30310 | 0.81036 | 1.20E-50 | -1.69E-46 |
| MS.gene055369 | MS.gene30310 | 0.805566 | 1.25E-49 | -1.69E-46 |
| MS.gene056346 | MS.gene30310 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene056514 | MS.gene30310 | 0.826886 | 2.12E-54 | -1.69E-46 |
| MS.gene057887 | MS.gene30310 | 0.84235 | 2.73E-58 | -1.69E-46 |
| MS.gene059420 | MS.gene30310 | 0.816162 | 6.38E-52 | -1.69E-46 |
| MS.gene059517 | MS.gene30310 | 0.827478 | 1.53E-54 | -1.69E-46 |
| MS.gene061214 | MS.gene30310 | 0.806808 | 6.87E-50 | -1.69E-46 |
| MS.gene061215 | MS.gene30310 | 0.804748 | 1.86E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30310.t1 | MTR_8g019450 | 43.725 | 247 | 88 | 7 | 14 | 213 | 6 | 248 | 7.00e-52 | 168 |
| MS.gene30310.t1 | MTR_0514s0010 | 40.486 | 247 | 94 | 6 | 13 | 213 | 8 | 247 | 1.92e-49 | 161 |
| MS.gene30310.t1 | MTR_8g018980 | 41.921 | 229 | 84 | 5 | 17 | 199 | 10 | 235 | 6.43e-49 | 160 |
| MS.gene30310.t1 | MTR_0470s0020 | 40.081 | 247 | 98 | 6 | 13 | 213 | 6 | 248 | 2.29e-47 | 156 |
| MS.gene30310.t1 | MTR_8g019050 | 40.081 | 247 | 98 | 6 | 13 | 213 | 6 | 248 | 2.29e-47 | 156 |
| MS.gene30310.t1 | MTR_8g019440 | 42.697 | 178 | 63 | 3 | 56 | 199 | 1 | 173 | 2.75e-43 | 144 |
| MS.gene30310.t1 | MTR_8g019060 | 36.245 | 229 | 109 | 6 | 6 | 202 | 42 | 265 | 1.32e-39 | 141 |
| MS.gene30310.t1 | MTR_8g019060 | 40.217 | 184 | 72 | 5 | 43 | 192 | 267 | 446 | 6.76e-36 | 131 |
| MS.gene30310.t1 | MTR_8g019380 | 38.776 | 245 | 102 | 9 | 12 | 213 | 4 | 243 | 1.07e-36 | 129 |
| MS.gene30310.t1 | MTR_8g019370 | 36.471 | 170 | 71 | 4 | 74 | 210 | 1 | 166 | 4.28e-29 | 107 |
| MS.gene30310.t1 | MTR_8g019290 | 29.825 | 171 | 83 | 4 | 45 | 184 | 6 | 170 | 1.54e-21 | 87.8 |
| MS.gene30310.t1 | MTR_8g019260 | 33.103 | 145 | 59 | 3 | 45 | 159 | 6 | 142 | 1.69e-19 | 82.4 |
| MS.gene30310.t1 | MTR_8g019110 | 33.103 | 145 | 59 | 3 | 45 | 159 | 6 | 142 | 1.69e-19 | 82.4 |
| MS.gene30310.t1 | MTR_1g069110 | 27.948 | 229 | 103 | 7 | 3 | 189 | 226 | 434 | 1.44e-16 | 77.8 |
| MS.gene30310.t1 | MTR_0470s0010 | 27.564 | 156 | 85 | 1 | 45 | 172 | 8 | 163 | 8.43e-15 | 70.1 |
| MS.gene30310.t1 | MTR_8g019020 | 27.564 | 156 | 85 | 1 | 45 | 172 | 8 | 163 | 8.43e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAATAAGGTTAGTTATT+TGG | 0.107289 | 8.3:-71391896 | MS.gene30310:CDS |
| CACAATAAGGTTAGTTATTT+GGG | 0.139676 | 8.3:-71391895 | MS.gene30310:CDS |
| ATTGGATTGTTATCAATTCT+TGG | 0.282494 | 8.3:-71391927 | MS.gene30310:CDS |
| GCTTCTCCGGGACATAGTTT+TGG | 0.283427 | 8.3:+71392215 | None:intergenic |
| AGGAACCTATGATAATCATT+AGG | 0.312240 | 8.3:+71392173 | None:intergenic |
| AAAGAGTACAAAGGAGATTT+CGG | 0.335902 | 8.3:-71391826 | MS.gene30310:CDS |
| AAGTACATTGTAGCAAAATC+AGG | 0.342618 | 8.3:-71392428 | MS.gene30310:CDS |
| CCTTTCTTTGAACTTCTTGT+CGG | 0.359494 | 8.3:+71392458 | None:intergenic |
| TTGGTTTGTGCCTCACAATA+AGG | 0.374489 | 8.3:-71391908 | MS.gene30310:CDS |
| ATGTCCCGGAGAAGCTGTTT+TGG | 0.376789 | 8.3:-71392207 | MS.gene30310:CDS |
| ATAACCCAAATGGATAATTC+TGG | 0.383482 | 8.3:-71392533 | None:intergenic |
| TATACCCTAATGATTATCAT+AGG | 0.385433 | 8.3:-71392178 | MS.gene30310:intron |
| TCATCGCCGTTGTCTTTGTC+TGG | 0.386438 | 8.3:+71392239 | None:intergenic |
| TAACAAGTTTCTTGAGAAGT+TGG | 0.386760 | 8.3:-71391961 | MS.gene30310:CDS |
| CCTATGTGGATGTTAGCAAT+AGG | 0.389609 | 8.3:-71392068 | MS.gene30310:CDS |
| AATAGCCAAAACAGCTTCTC+CGG | 0.412499 | 8.3:+71392202 | None:intergenic |
| GGAACCTATGATAATCATTA+GGG | 0.412647 | 8.3:+71392174 | None:intergenic |
| AACTTCTTCTTCATGTGCTA+TGG | 0.417595 | 8.3:+71392499 | None:intergenic |
| TAGCAAAATCAGGGCACCTT+TGG | 0.421626 | 8.3:-71392418 | MS.gene30310:CDS |
| GTTAGACCAGTAAAAGAAAC+AGG | 0.427303 | 8.3:-71392281 | MS.gene30310:CDS |
| TGACTGTTGCTATTAAACTT+AGG | 0.443404 | 8.3:+71392348 | None:intergenic |
| CTCAACAATGACATTTCAAA+TGG | 0.459232 | 8.3:-71392324 | MS.gene30310:CDS |
| TTTCCTCAACAGAAGTAAGA+TGG | 0.459688 | 8.3:-71391862 | MS.gene30310:CDS |
| TGTCTTCTTCATTCATCCAA+AGG | 0.467265 | 8.3:+71392402 | None:intergenic |
| AAGGTTAGTTATTTGGGGAG+TGG | 0.468986 | 8.3:-71391889 | MS.gene30310:CDS |
| ACATGAAGAAGAAGTTGGTC+AGG | 0.478107 | 8.3:-71392492 | MS.gene30310:CDS |
| CAAGAAGTTCAAAGAAAGGT+TGG | 0.487887 | 8.3:-71392454 | MS.gene30310:CDS |
| CAAATGGCGTTTAATCCACC+AGG | 0.491341 | 8.3:-71392308 | MS.gene30310:CDS |
| CTATGTGGATGTTAGCAATA+GGG | 0.500543 | 8.3:-71392067 | MS.gene30310:CDS |
| TCATGTGCTATGGATTGTGT+TGG | 0.508903 | 8.3:+71392509 | None:intergenic |
| ATGAAGAAGACAAGAAAGAA+TGG | 0.521764 | 8.3:-71392391 | MS.gene30310:CDS |
| TTTACTGGTCTAACACCACC+TGG | 0.544912 | 8.3:+71392290 | None:intergenic |
| TAGCCATCTTACTTCTGTTG+AGG | 0.559009 | 8.3:+71391859 | None:intergenic |
| ATAGCACATGAAGAAGAAGT+TGG | 0.565206 | 8.3:-71392497 | MS.gene30310:CDS |
| TTGTCTTTGTCTGGAAGACA+TGG | 0.568346 | 8.3:+71392248 | None:intergenic |
| ACAATAAGGTTAGTTATTTG+GGG | 0.580656 | 8.3:-71391894 | MS.gene30310:CDS |
| TGCATCATAGGTTACCTATG+TGG | 0.581559 | 8.3:-71392082 | MS.gene30310:intron |
| ATAGCCAAAACAGCTTCTCC+GGG | 0.585715 | 8.3:+71392203 | None:intergenic |
| CCGACAAGAAGTTCAAAGAA+AGG | 0.595030 | 8.3:-71392458 | MS.gene30310:CDS |
| GAATGGTTAAACTACATGAG+AGG | 0.596068 | 8.3:-71392374 | MS.gene30310:CDS |
| AGTACATTGTAGCAAAATCA+GGG | 0.596650 | 8.3:-71392427 | MS.gene30310:CDS |
| AATGGTTAAACTACATGAGA+GGG | 0.596824 | 8.3:-71392373 | MS.gene30310:CDS |
| CCTATTGCTAACATCCACAT+AGG | 0.600445 | 8.3:+71392068 | None:intergenic |
| GATGACCCAAAACTATGTCC+CGG | 0.622580 | 8.3:-71392221 | MS.gene30310:CDS |
| AAATAACTAACCTTATTGTG+AGG | 0.630050 | 8.3:+71391898 | None:intergenic |
| ACTGGTCTAACACCACCTGG+TGG | 0.656114 | 8.3:+71392293 | None:intergenic |
| GACTGAACAAAAGAGTACAA+AGG | 0.662802 | 8.3:-71391835 | MS.gene30310:CDS |
| TGTCTTCCAGACAAAGACAA+CGG | 0.729308 | 8.3:-71392245 | MS.gene30310:CDS |
| ATGGCGTTTAATCCACCAGG+TGG | 0.730048 | 8.3:-71392305 | MS.gene30310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATTGTGTTTGAAATA+AGG | + | chr8.3:71392195-71392214 | None:intergenic | 20.0% |
| ! | AAATAACTAACCTTATTGTG+AGG | + | chr8.3:71392450-71392469 | None:intergenic | 25.0% |
| ! | ATTGGATTGTTATCAATTCT+TGG | - | chr8.3:71392418-71392437 | MS.gene30310:CDS | 25.0% |
| ! | TATACCCTAATGATTATCAT+AGG | - | chr8.3:71392167-71392186 | MS.gene30310:intron | 25.0% |
| !! | ACAATAAGGTTAGTTATTTG+GGG | - | chr8.3:71392451-71392470 | MS.gene30310:CDS | 25.0% |
| !! | CACAATAAGGTTAGTTATTT+GGG | - | chr8.3:71392450-71392469 | MS.gene30310:CDS | 25.0% |
| !! | TCACAATAAGGTTAGTTATT+TGG | - | chr8.3:71392449-71392468 | MS.gene30310:CDS | 25.0% |
| !!! | TTGTTAGCTTTTTCAAAAAC+AGG | + | chr8.3:71392370-71392389 | None:intergenic | 25.0% |
| AAGTACATTGTAGCAAAATC+AGG | - | chr8.3:71391917-71391936 | MS.gene30310:CDS | 30.0% | |
| AATGGTTAAACTACATGAGA+GGG | - | chr8.3:71391972-71391991 | MS.gene30310:CDS | 30.0% | |
| AGGAACCTATGATAATCATT+AGG | + | chr8.3:71392175-71392194 | None:intergenic | 30.0% | |
| AGTACATTGTAGCAAAATCA+GGG | - | chr8.3:71391918-71391937 | MS.gene30310:CDS | 30.0% | |
| ATGAAGAAGACAAGAAAGAA+TGG | - | chr8.3:71391954-71391973 | MS.gene30310:CDS | 30.0% | |
| CTCAACAATGACATTTCAAA+TGG | - | chr8.3:71392021-71392040 | MS.gene30310:CDS | 30.0% | |
| GGAACCTATGATAATCATTA+GGG | + | chr8.3:71392174-71392193 | None:intergenic | 30.0% | |
| TAACAAGTTTCTTGAGAAGT+TGG | - | chr8.3:71392384-71392403 | MS.gene30310:CDS | 30.0% | |
| ! | AAAGAGTACAAAGGAGATTT+CGG | - | chr8.3:71392519-71392538 | MS.gene30310:CDS | 30.0% |
| ! | TGACTGTTGCTATTAAACTT+AGG | + | chr8.3:71392000-71392019 | None:intergenic | 30.0% |
| !! | TCATTTCCTGTTTCTTTTAC+TGG | + | chr8.3:71392073-71392092 | None:intergenic | 30.0% |
| !!! | AAGTTGGTTTTTACTTGGAT+TGG | - | chr8.3:71392400-71392419 | MS.gene30310:CDS | 30.0% |
| !!! | TTGAGAAGTTGGTTTTTACT+TGG | - | chr8.3:71392395-71392414 | MS.gene30310:CDS | 30.0% |
| AACTTCTTCTTCATGTGCTA+TGG | + | chr8.3:71391849-71391868 | None:intergenic | 35.0% | |
| ATAGCACATGAAGAAGAAGT+TGG | - | chr8.3:71391848-71391867 | MS.gene30310:CDS | 35.0% | |
| CAAGAAGTTCAAAGAAAGGT+TGG | - | chr8.3:71391891-71391910 | MS.gene30310:CDS | 35.0% | |
| CCTTTCTTTGAACTTCTTGT+CGG | + | chr8.3:71391890-71391909 | None:intergenic | 35.0% | |
| CTATGTGGATGTTAGCAATA+GGG | - | chr8.3:71392278-71392297 | MS.gene30310:CDS | 35.0% | |
| GAATGGTTAAACTACATGAG+AGG | - | chr8.3:71391971-71391990 | MS.gene30310:CDS | 35.0% | |
| GTTAGACCAGTAAAAGAAAC+AGG | - | chr8.3:71392064-71392083 | MS.gene30310:CDS | 35.0% | |
| TGTCTTCTTCATTCATCCAA+AGG | + | chr8.3:71391946-71391965 | None:intergenic | 35.0% | |
| TTTCCTCAACAGAAGTAAGA+TGG | - | chr8.3:71392483-71392502 | MS.gene30310:CDS | 35.0% | |
| ! | GACTGAACAAAAGAGTACAA+AGG | - | chr8.3:71392510-71392529 | MS.gene30310:CDS | 35.0% |
| !!! | GCTTTTTCAAAAACAGGATC+TGG | + | chr8.3:71392364-71392383 | None:intergenic | 35.0% |
| AATAGCCAAAACAGCTTCTC+CGG | + | chr8.3:71392146-71392165 | None:intergenic | 40.0% | |
| ATTTGTCTCTTGCTTGTGAG+TGG | - | chr8.3:71392232-71392251 | MS.gene30310:CDS | 40.0% | |
| CCGACAAGAAGTTCAAAGAA+AGG | - | chr8.3:71391887-71391906 | MS.gene30310:CDS | 40.0% | |
| CCTATGTGGATGTTAGCAAT+AGG | - | chr8.3:71392277-71392296 | MS.gene30310:CDS | 40.0% | |
| CCTATTGCTAACATCCACAT+AGG | + | chr8.3:71392280-71392299 | None:intergenic | 40.0% | |
| GTGGAATTCGTTTGCATCAT+AGG | - | chr8.3:71392251-71392270 | MS.gene30310:CDS | 40.0% | |
| TAGCCATCTTACTTCTGTTG+AGG | + | chr8.3:71392489-71392508 | None:intergenic | 40.0% | |
| TGCATCATAGGTTACCTATG+TGG | - | chr8.3:71392263-71392282 | MS.gene30310:CDS | 40.0% | |
| TGTCTTCCAGACAAAGACAA+CGG | - | chr8.3:71392100-71392119 | MS.gene30310:intron | 40.0% | |
| TTGTCTTTGTCTGGAAGACA+TGG | + | chr8.3:71392100-71392119 | None:intergenic | 40.0% | |
| ! | AAGGTTAGTTATTTGGGGAG+TGG | - | chr8.3:71392456-71392475 | MS.gene30310:CDS | 40.0% |
| ! | ACATGAAGAAGAAGTTGGTC+AGG | - | chr8.3:71391853-71391872 | MS.gene30310:CDS | 40.0% |
| ! | TCATGTGCTATGGATTGTGT+TGG | + | chr8.3:71391839-71391858 | None:intergenic | 40.0% |
| !! | TTGGTTTGTGCCTCACAATA+AGG | - | chr8.3:71392437-71392456 | MS.gene30310:CDS | 40.0% |
| ATAGCCAAAACAGCTTCTCC+GGG | + | chr8.3:71392145-71392164 | None:intergenic | 45.0% | |
| CAAATGGCGTTTAATCCACC+AGG | - | chr8.3:71392037-71392056 | MS.gene30310:CDS | 45.0% | |
| GATGACCCAAAACTATGTCC+CGG | - | chr8.3:71392124-71392143 | MS.gene30310:intron | 45.0% | |
| ! | CTTCTCCGGGACATAGTTTT+GGG | + | chr8.3:71392132-71392151 | None:intergenic | 45.0% |
| ! | TAGCAAAATCAGGGCACCTT+TGG | - | chr8.3:71391927-71391946 | MS.gene30310:CDS | 45.0% |
| ! | TTTACTGGTCTAACACCACC+TGG | + | chr8.3:71392058-71392077 | None:intergenic | 45.0% |
| ATGGCGTTTAATCCACCAGG+TGG | - | chr8.3:71392040-71392059 | MS.gene30310:CDS | 50.0% | |
| TCATCGCCGTTGTCTTTGTC+TGG | + | chr8.3:71392109-71392128 | None:intergenic | 50.0% | |
| ! | ATGTCCCGGAGAAGCTGTTT+TGG | - | chr8.3:71392138-71392157 | MS.gene30310:intron | 50.0% |
| ! | GCTTCTCCGGGACATAGTTT+TGG | + | chr8.3:71392133-71392152 | None:intergenic | 50.0% |
| ! | ACTGGTCTAACACCACCTGG+TGG | + | chr8.3:71392055-71392074 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 71391821 | 71392546 | 71391821 | ID=MS.gene30310 |
| chr8.3 | mRNA | 71391821 | 71392546 | 71391821 | ID=MS.gene30310.t1;Parent=MS.gene30310 |
| chr8.3 | exon | 71392179 | 71392546 | 71392179 | ID=MS.gene30310.t1.exon1;Parent=MS.gene30310.t1 |
| chr8.3 | CDS | 71392179 | 71392546 | 71392179 | ID=cds.MS.gene30310.t1;Parent=MS.gene30310.t1 |
| chr8.3 | exon | 71391821 | 71392094 | 71391821 | ID=MS.gene30310.t1.exon2;Parent=MS.gene30310.t1 |
| chr8.3 | CDS | 71391821 | 71392094 | 71391821 | ID=cds.MS.gene30310.t1;Parent=MS.gene30310.t1 |
| Gene Sequence |
| Protein sequence |
