Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30486.t1 | KEH17045.1 | 92.4 | 118 | 8 | 1 | 1 | 118 | 57 | 173 | 6.10E-54 | 219.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30486.t1 | Q9FLI7 | 75.6 | 82 | 18 | 1 | 37 | 118 | 28 | 107 | 8.2e-31 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30486.t1 | A0A072TU10 | 92.4 | 118 | 8 | 1 | 1 | 118 | 57 | 173 | 4.4e-54 | 219.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene30486 | 0.828902 | 6.94E-55 | -1.69E-46 |
| MS.gene050128 | MS.gene30486 | 0.80293 | 4.44E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene30486 | 0.801947 | 7.08E-49 | -1.69E-46 |
| MS.gene054542 | MS.gene30486 | 0.806691 | 7.28E-50 | -1.69E-46 |
| MS.gene060084 | MS.gene30486 | 0.82367 | 1.22E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30486.t1 | MTR_0050s0180 | 92.373 | 118 | 8 | 1 | 1 | 118 | 57 | 173 | 5.76e-75 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30486.t1 | AT5G51720 | 75.610 | 82 | 18 | 1 | 37 | 118 | 28 | 107 | 5.04e-41 | 132 |
Find 32 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCATTCTTCCTTATATC+TGG | 0.199944 | 6.4:+41714219 | None:intergenic |
| AAAGGAACCAGTGTTGAAAT+TGG | 0.358391 | 6.4:+41714311 | None:intergenic |
| AACACTGGTTCCTTTGGTAT+TGG | 0.361195 | 6.4:-41714303 | MS.gene30486:CDS |
| TCTCACCTAAACGTGGCTTT+TGG | 0.382823 | 6.4:+41714355 | None:intergenic |
| AATTTCAACACTGGTTCCTT+TGG | 0.387674 | 6.4:-41714309 | MS.gene30486:CDS |
| GTGGTGGTGAAAGCAGAAAC+AGG | 0.396412 | 6.4:-41714261 | MS.gene30486:CDS |
| TGGCTTTGTTGTGCTTTACA+TGG | 0.398604 | 6.4:+41712519 | None:intergenic |
| ACTGAACCATACCTGCAATA+AGG | 0.398811 | 6.4:+41714147 | None:intergenic |
| AGGCTGTTACTCCTTATTGC+AGG | 0.410961 | 6.4:-41714158 | MS.gene30486:intron |
| TTGGTATTGGTAATAAAGTA+AGG | 0.429418 | 6.4:-41714290 | MS.gene30486:CDS |
| GTAGTATAAATCCAGATATA+AGG | 0.442758 | 6.4:-41714230 | MS.gene30486:CDS |
| TATAAGGAAGAATGAAGAAA+AGG | 0.447347 | 6.4:-41714214 | MS.gene30486:CDS |
| ATGTGCCAAAAGCCACGTTT+AGG | 0.452497 | 6.4:-41714360 | MS.gene30486:CDS |
| TGATACAGATGCTGGAGATC+AGG | 0.452783 | 6.4:-41712566 | MS.gene30486:intron |
| GGCACATAACTACACCAACT+TGG | 0.479160 | 6.4:+41714376 | None:intergenic |
| AAGCCACTGGAGATAATGTT+GGG | 0.514803 | 6.4:-41712502 | MS.gene30486:CDS |
| AAAGCCACTGGAGATAATGT+TGG | 0.533290 | 6.4:-41712503 | MS.gene30486:CDS |
| TACTTTATTACCAATACCAA+AGG | 0.542212 | 6.4:+41714293 | None:intergenic |
| TGTGCTCAGTGAAGCGTCCA+AGG | 0.551440 | 6.4:-41714178 | MS.gene30486:CDS |
| GGAACCTTTCCTCTGTGTGA+TGG | 0.562888 | 6.4:-41712545 | MS.gene30486:CDS |
| TAAAGTAAGGAGTGTGGTGG+TGG | 0.565710 | 6.4:-41714277 | MS.gene30486:CDS |
| GTAGGTACCAATTTCAACAC+TGG | 0.571557 | 6.4:-41714318 | MS.gene30486:CDS |
| GTAAAGCACAACAAAGCCAC+TGG | 0.579483 | 6.4:-41712515 | MS.gene30486:CDS |
| GCAATAAGGAGTAACAGCCT+TGG | 0.590907 | 6.4:+41714161 | None:intergenic |
| TGGTGGTGAAAGCAGAAACA+GGG | 0.606827 | 6.4:-41714260 | MS.gene30486:CDS |
| GCTTCCATCACACAGAGGAA+AGG | 0.616730 | 6.4:+41712541 | None:intergenic |
| AGGCCCAACATTATCTCCAG+TGG | 0.629442 | 6.4:+41712499 | None:intergenic |
| TGGTAATAAAGTAAGGAGTG+TGG | 0.632456 | 6.4:-41714283 | MS.gene30486:CDS |
| GGTGGTGAAAGCAGAAACAG+GGG | 0.695599 | 6.4:-41714259 | MS.gene30486:CDS |
| TAATAAAGTAAGGAGTGTGG+TGG | 0.737584 | 6.4:-41714280 | MS.gene30486:CDS |
| GTGGTGAAAGCAGAAACAGG+GGG | 0.738994 | 6.4:-41714258 | MS.gene30486:CDS |
| ACATGGCTTCCATCACACAG+AGG | 0.761842 | 6.4:+41712536 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAATAATTTAGTTTGTT+TGG | + | chr6.4:41713289-41713308 | None:intergenic | 10.0% |
| !!! | TAAAAAATAGATTTTTTTAG+AGG | - | chr6.4:41713185-41713204 | MS.gene30486:intron | 10.0% |
| !!! | TTATGTTTTTCATTTATTTT+TGG | - | chr6.4:41712753-41712772 | MS.gene30486:intron | 10.0% |
| !! | CAATAATCAAATTTCAATAA+TGG | + | chr6.4:41712875-41712894 | None:intergenic | 15.0% |
| !! | CATATGATAAAGATATAATA+AGG | + | chr6.4:41714060-41714079 | None:intergenic | 15.0% |
| !! | CATTATTGAAATTTGATTAT+TGG | - | chr6.4:41712873-41712892 | MS.gene30486:intron | 15.0% |
| !!! | TTTAAATTATCACTTTTAAG+AGG | - | chr6.4:41713138-41713157 | MS.gene30486:intron | 15.0% |
| !! | AAAAATTTGGTACTTATTAG+TGG | - | chr6.4:41713959-41713978 | MS.gene30486:intron | 20.0% |
| !! | AAAACAAATCTTAATCTTTC+TGG | - | chr6.4:41714273-41714292 | MS.gene30486:CDS | 20.0% |
| !! | ATAGATTCCACATAAAAATT+TGG | - | chr6.4:41713946-41713965 | MS.gene30486:intron | 20.0% |
| !! | ATTAACATTTGCTTCATATT+AGG | - | chr6.4:41713438-41713457 | MS.gene30486:intron | 20.0% |
| !! | GTTCTTGAGTAATTATAATA+AGG | + | chr6.4:41714139-41714158 | None:intergenic | 20.0% |
| !!! | AGAAAGATTAAGATTTGTTT+TGG | + | chr6.4:41714274-41714293 | None:intergenic | 20.0% |
| !!! | ATAAGTACCAAATTTTTATG+TGG | + | chr6.4:41713956-41713975 | None:intergenic | 20.0% |
| !!! | TGTCAAATTTTCACATTAAA+AGG | + | chr6.4:41713030-41713049 | None:intergenic | 20.0% |
| !!! | TTGATTATTGGTTAATTATG+TGG | - | chr6.4:41712885-41712904 | MS.gene30486:intron | 20.0% |
| !!! | TTTTTAACCAATAGCAAAAA+TGG | + | chr6.4:41712838-41712857 | None:intergenic | 20.0% |
| ! | AAGCTATACTAGATAGTTTA+TGG | - | chr6.4:41712927-41712946 | MS.gene30486:intron | 25.0% |
| ! | ACATAGACATGATTTATTTG+TGG | + | chr6.4:41713770-41713789 | None:intergenic | 25.0% |
| ! | ATTCAGTTGAAAACAACTTA+TGG | - | chr6.4:41712951-41712970 | MS.gene30486:intron | 25.0% |
| ! | CAAATCTTATCCCATTAAAT+AGG | + | chr6.4:41713913-41713932 | None:intergenic | 25.0% |
| ! | CTATTTAATGGGATAAGATT+TGG | - | chr6.4:41713911-41713930 | MS.gene30486:intron | 25.0% |
| ! | GTAGTATAAATCCAGATATA+AGG | - | chr6.4:41712644-41712663 | MS.gene30486:intron | 25.0% |
| ! | TACTTTATTACCAATACCAA+AGG | + | chr6.4:41712584-41712603 | None:intergenic | 25.0% |
| ! | TATAAGGAAGAATGAAGAAA+AGG | - | chr6.4:41712660-41712679 | MS.gene30486:intron | 25.0% |
| ! | TATCACAAATTACCAAAGTT+TGG | - | chr6.4:41713606-41713625 | MS.gene30486:intron | 25.0% |
| !! | ATCATAATCTAGAGTAATGT+CGG | - | chr6.4:41713688-41713707 | MS.gene30486:intron | 25.0% |
| !!! | AATCTTAATCTTTCTGGTTA+TGG | - | chr6.4:41714279-41714298 | MS.gene30486:CDS | 25.0% |
| !!! | ACACAATTTTAACCAAACTT+TGG | + | chr6.4:41713621-41713640 | None:intergenic | 25.0% |
| !!! | ATCTTAATCTTTCTGGTTAT+GGG | - | chr6.4:41714280-41714299 | MS.gene30486:CDS | 25.0% |
| !!! | TTGGTATTGGTAATAAAGTA+AGG | - | chr6.4:41712584-41712603 | MS.gene30486:intron | 25.0% |
| ACTTCAAAACATGAAGAACA+AGG | + | chr6.4:41714162-41714181 | None:intergenic | 30.0% | |
| CTTATCCCATTAAATAGGAT+TGG | + | chr6.4:41713908-41713927 | None:intergenic | 30.0% | |
| CTTCAAAACATGAAGAACAA+GGG | + | chr6.4:41714161-41714180 | None:intergenic | 30.0% | |
| GAGAGAAAAAATGGAATTGT+AGG | - | chr6.4:41712538-41712557 | MS.gene30486:CDS | 30.0% | |
| GCTTCTATAATATATGTCAG+TGG | - | chr6.4:41714015-41714034 | MS.gene30486:intron | 30.0% | |
| TCTTCATTCTTCCTTATATC+TGG | + | chr6.4:41712658-41712677 | None:intergenic | 30.0% | |
| TGATTGATTACATCTGTTTC+TGG | - | chr6.4:41713530-41713549 | MS.gene30486:intron | 30.0% | |
| TTTGGTTAAGTGTATCATTG+TGG | - | chr6.4:41712771-41712790 | MS.gene30486:intron | 30.0% | |
| ! | AACTTTAATGTTGATTGTCG+AGG | + | chr6.4:41713852-41713871 | None:intergenic | 30.0% |
| ! | TCATAATCTAGAGTAATGTC+GGG | - | chr6.4:41713689-41713708 | MS.gene30486:intron | 30.0% |
| !! | GCATTTACCATTTTTGCTAT+TGG | - | chr6.4:41712828-41712847 | MS.gene30486:intron | 30.0% |
| AAAAGGAGAGAGTTGTGTTT+AGG | - | chr6.4:41713826-41713845 | MS.gene30486:intron | 35.0% | |
| ACGAGCCAATCCTATTTAAT+GGG | - | chr6.4:41713900-41713919 | MS.gene30486:intron | 35.0% | |
| CGTTTAGGTGAGAGAAAAAA+TGG | - | chr6.4:41712529-41712548 | MS.gene30486:CDS | 35.0% | |
| GTTTATCTACTGACATAGAG+AGG | + | chr6.4:41712985-41713004 | None:intergenic | 35.0% | |
| TACGAGCCAATCCTATTTAA+TGG | - | chr6.4:41713899-41713918 | MS.gene30486:intron | 35.0% | |
| TCATATTAGGTGAACGATTC+TGG | - | chr6.4:41713451-41713470 | MS.gene30486:intron | 35.0% | |
| TGGTAATAAAGTAAGGAGTG+TGG | - | chr6.4:41712591-41712610 | MS.gene30486:intron | 35.0% | |
| ! | AAAGGAACCAGTGTTGAAAT+TGG | + | chr6.4:41712566-41712585 | None:intergenic | 35.0% |
| ! | ACAACTCTCTCCTTTTACTT+CGG | + | chr6.4:41713822-41713841 | None:intergenic | 35.0% |
| ! | CATAATCTAGAGTAATGTCG+GGG | - | chr6.4:41713690-41713709 | MS.gene30486:intron | 35.0% |
| ! | TAATAAAGTAAGGAGTGTGG+TGG | - | chr6.4:41712594-41712613 | MS.gene30486:intron | 35.0% |
| !! | AAATAGGATTGGCTCGTATT+TGG | + | chr6.4:41713897-41713916 | None:intergenic | 35.0% |
| !! | AATTTCAACACTGGTTCCTT+TGG | - | chr6.4:41712565-41712584 | MS.gene30486:intron | 35.0% |
| !! | TTAGCCTTGTTATACATTGC+CGG | - | chr6.4:41713789-41713808 | MS.gene30486:intron | 35.0% |
| !!! | TTTTTTCTCTCACCTAAACG+TGG | + | chr6.4:41712529-41712548 | None:intergenic | 35.0% |
| AAAGCCACTGGAGATAATGT+TGG | - | chr6.4:41714371-41714390 | MS.gene30486:CDS | 40.0% | |
| AAGCCACTGGAGATAATGTT+GGG | - | chr6.4:41714372-41714391 | MS.gene30486:CDS | 40.0% | |
| ACTGAACCATACCTGCAATA+AGG | + | chr6.4:41712730-41712749 | None:intergenic | 40.0% | |
| GTAGGTACCAATTTCAACAC+TGG | - | chr6.4:41712556-41712575 | MS.gene30486:CDS | 40.0% | |
| GTGGAACATATCAACATCGT+AGG | + | chr6.4:41713751-41713770 | None:intergenic | 40.0% | |
| GTTACTCCTTATTGCAGGTA+TGG | - | chr6.4:41712721-41712740 | MS.gene30486:intron | 40.0% | |
| ! | CTCTCCTTTTACTTCGGTTT+GGG | + | chr6.4:41713816-41713835 | None:intergenic | 40.0% |
| ! | TCTCTCCTTTTACTTCGGTT+TGG | + | chr6.4:41713817-41713836 | None:intergenic | 40.0% |
| !! | AACACTGGTTCCTTTGGTAT+TGG | - | chr6.4:41712571-41712590 | MS.gene30486:intron | 40.0% |
| !! | ATTACATCTGTTTCTGGACG+TGG | - | chr6.4:41713536-41713555 | MS.gene30486:intron | 40.0% |
| !! | GCATCTGTTTTAAGCCAAGT+TGG | - | chr6.4:41712484-41712503 | MS.gene30486:CDS | 40.0% |
| !! | TGGCTTTGTTGTGCTTTACA+TGG | + | chr6.4:41714358-41714377 | None:intergenic | 40.0% |
| AGGCTGTTACTCCTTATTGC+AGG | - | chr6.4:41712716-41712735 | MS.gene30486:intron | 45.0% | |
| ATGTGCCAAAAGCCACGTTT+AGG | - | chr6.4:41712514-41712533 | MS.gene30486:CDS | 45.0% | |
| GCAATAAGGAGTAACAGCCT+TGG | + | chr6.4:41712716-41712735 | None:intergenic | 45.0% | |
| GGCACATAACTACACCAACT+TGG | + | chr6.4:41712501-41712520 | None:intergenic | 45.0% | |
| GTAAAGCACAACAAAGCCAC+TGG | - | chr6.4:41714359-41714378 | MS.gene30486:CDS | 45.0% | |
| TGATACAGATGCTGGAGATC+AGG | - | chr6.4:41714308-41714327 | MS.gene30486:CDS | 45.0% | |
| ! | TAAAGTAAGGAGTGTGGTGG+TGG | - | chr6.4:41712597-41712616 | MS.gene30486:intron | 45.0% |
| ! | TCTCACCTAAACGTGGCTTT+TGG | + | chr6.4:41712522-41712541 | None:intergenic | 45.0% |
| ! | TGGTGGTGAAAGCAGAAACA+GGG | - | chr6.4:41712614-41712633 | MS.gene30486:intron | 45.0% |
| !!! | CTTTTACTTCGGTTTGGGAC+CGG | + | chr6.4:41713811-41713830 | None:intergenic | 45.0% |
| ACATGGCTTCCATCACACAG+AGG | + | chr6.4:41714341-41714360 | None:intergenic | 50.0% | |
| AGGCCCAACATTATCTCCAG+TGG | + | chr6.4:41714378-41714397 | None:intergenic | 50.0% | |
| CGGTCCCAAACCGAAGTAAA+AGG | - | chr6.4:41713809-41713828 | MS.gene30486:intron | 50.0% | |
| GCTTCCATCACACAGAGGAA+AGG | + | chr6.4:41714336-41714355 | None:intergenic | 50.0% | |
| GGAACCTTTCCTCTGTGTGA+TGG | - | chr6.4:41714329-41714348 | MS.gene30486:CDS | 50.0% | |
| GGGACCGGCAATGTATAACA+AGG | + | chr6.4:41713796-41713815 | None:intergenic | 50.0% | |
| GGGTGCTTTGATACAGATGC+TGG | - | chr6.4:41714300-41714319 | MS.gene30486:CDS | 50.0% | |
| GGTGGTGAAAGCAGAAACAG+GGG | - | chr6.4:41712615-41712634 | MS.gene30486:intron | 50.0% | |
| GTGGTGAAAGCAGAAACAGG+GGG | - | chr6.4:41712616-41712635 | MS.gene30486:intron | 50.0% | |
| ! | GTGGTGGTGAAAGCAGAAAC+AGG | - | chr6.4:41712613-41712632 | MS.gene30486:intron | 50.0% |
| ! | TGTGCTCAGTGAAGCGTCCA+AGG | - | chr6.4:41712696-41712715 | MS.gene30486:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 41712481 | 41714415 | 41712481 | ID=MS.gene30486 |
| chr6.4 | mRNA | 41712481 | 41714415 | 41712481 | ID=MS.gene30486.t1;Parent=MS.gene30486 |
| chr6.4 | exon | 41714159 | 41714415 | 41714159 | ID=MS.gene30486.t1.exon1;Parent=MS.gene30486.t1 |
| chr6.4 | CDS | 41714159 | 41714415 | 41714159 | ID=cds.MS.gene30486.t1;Parent=MS.gene30486.t1 |
| chr6.4 | exon | 41712481 | 41712580 | 41712481 | ID=MS.gene30486.t1.exon2;Parent=MS.gene30486.t1 |
| chr6.4 | CDS | 41712481 | 41712580 | 41712481 | ID=cds.MS.gene30486.t1;Parent=MS.gene30486.t1 |
| Gene Sequence |
| Protein sequence |
