AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054542


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054542.t1 MTR_4g007550 86.555 119 13 1 1 116 1 119 1.81e-62 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTAAGATGTAGGAAACTA+TGG 0.353441 4.1:+87845163 MS.gene054542:intron
GCTCTACATCCAAGTTATCT+TGG 0.366777 4.1:+87845341 MS.gene054542:CDS
AGACCTAACACGGTTCGAAT+AGG 0.402827 4.1:-87845659 None:intergenic
ATTAAGAAAGCTAGAGGAAA+AGG 0.417119 4.1:+87845268 MS.gene054542:CDS
GACCTAACACGGTTCGAATA+GGG 0.446620 4.1:-87845658 None:intergenic
AGCCCTATTCGAACCGTGTT+AGG 0.457843 4.1:+87845656 MS.gene054542:CDS
GCAAGGGGAGAGAGTGAATA+CGG 0.507712 4.1:+87845682 MS.gene054542:CDS
GCTAGAGGAAAAGGAGCTAA+AGG 0.517100 4.1:+87845277 MS.gene054542:CDS
GAAGAATGCTTACTACTAGT+AGG 0.521612 4.1:-87845055 None:intergenic
AGGTGAAATCCAAGATAACT+TGG 0.531170 4.1:-87845350 None:intergenic
AACTATGGAAGCATAAAAGA+AGG 0.547768 4.1:+87845178 MS.gene054542:CDS
AGAAGATAAGAAACATATCA+AGG 0.562331 4.1:+87845216 MS.gene054542:CDS
CTCCCCTTGCAGACCTAACA+CGG 0.568523 4.1:-87845669 None:intergenic
ATTTCCAAATCCAATCAGAA+AGG 0.583110 4.1:+87845590 MS.gene054542:CDS
GAACCGTGTTAGGTCTGCAA+GGG 0.596457 4.1:+87845666 MS.gene054542:CDS
AAACTCGTCAAATAGCATCA+TGG 0.602558 4.1:+87845021 MS.gene054542:CDS
GACTCTATTAAGAAAGCTAG+AGG 0.614561 4.1:+87845262 MS.gene054542:CDS
ACGGTTCGAATAGGGCTACT+CGG 0.617568 4.1:-87845650 None:intergenic
CGAACCGTGTTAGGTCTGCA+AGG 0.628387 4.1:+87845665 MS.gene054542:CDS
AAAACAAAGCTTACACACAC+AGG 0.629592 4.1:-87845370 None:intergenic
AAAGAAGGAAGCAAGTTGCA+CGG 0.651204 4.1:+87845193 MS.gene054542:CDS
AACCGTGTTAGGTCTGCAAG+GGG 0.658848 4.1:+87845667 MS.gene054542:CDS
AGAGGAAAAGGAGCTAAAGG+TGG 0.683202 4.1:+87845280 MS.gene054542:CDS
AGATAAGAAACATATCAAGG+AGG 0.727302 4.1:+87845219 MS.gene054542:CDS
GTAGAGCTTCGAGAACGACG+AGG 0.744495 4.1:-87845325 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTCAAGTTATGAGAAATT+AGG - chr4.1:87845525-87845544 None:intergenic 20.0%
! AGAAGATAAGAAACATATCA+AGG + chr4.1:87845216-87845235 MS.gene054542:CDS 25.0%
! TATAGCATGAGTACAATTTA+AGG - chr4.1:87845101-87845120 None:intergenic 25.0%
! TATTAAGATGTAGGAAACTA+TGG + chr4.1:87845163-87845182 MS.gene054542:intron 25.0%
! TGCTTATTCTATTAAGATGT+AGG + chr4.1:87845154-87845173 MS.gene054542:intron 25.0%
AGATAAGAAACATATCAAGG+AGG + chr4.1:87845219-87845238 MS.gene054542:CDS 30.0%
ATTAAGAAAGCTAGAGGAAA+AGG + chr4.1:87845268-87845287 MS.gene054542:CDS 30.0%
ATTTCCAAATCCAATCAGAA+AGG + chr4.1:87845590-87845609 MS.gene054542:CDS 30.0%
TCATAGCAACTTAACATGAA+AGG - chr4.1:87845410-87845429 None:intergenic 30.0%
! AACTATGGAAGCATAAAAGA+AGG + chr4.1:87845178-87845197 MS.gene054542:CDS 30.0%
! ACATGTTTATGTTTGAAGCT+TGG + chr4.1:87845450-87845469 MS.gene054542:intron 30.0%
! CATGTTTATGTTTGAAGCTT+GGG + chr4.1:87845451-87845470 MS.gene054542:intron 30.0%
! TTTTCCTTTCTGATTGGATT+TGG - chr4.1:87845597-87845616 None:intergenic 30.0%
!!! ACTCAAGCTATATCGATTTT+AGG + chr4.1:87844974-87844993 MS.gene054542:CDS 30.0%
AAAACAAAGCTTACACACAC+AGG - chr4.1:87845373-87845392 None:intergenic 35.0%
AAACTCGTCAAATAGCATCA+TGG + chr4.1:87845021-87845040 MS.gene054542:CDS 35.0%
AGGTGAAATCCAAGATAACT+TGG - chr4.1:87845353-87845372 None:intergenic 35.0%
GAAGAATGCTTACTACTAGT+AGG - chr4.1:87845058-87845077 None:intergenic 35.0%
GACTCTATTAAGAAAGCTAG+AGG + chr4.1:87845262-87845281 MS.gene054542:CDS 35.0%
! CGATGATTTTCCTTTCTGAT+TGG - chr4.1:87845603-87845622 None:intergenic 35.0%
AAAGAAGGAAGCAAGTTGCA+CGG + chr4.1:87845193-87845212 MS.gene054542:CDS 40.0%
AAGGAAACGCTCCTTCAAAA+AGG - chr4.1:87845082-87845101 None:intergenic 40.0%
GCTCTACATCCAAGTTATCT+TGG + chr4.1:87845341-87845360 MS.gene054542:CDS 40.0%
! AGCATTCTTCGCCTTTTTGA+AGG + chr4.1:87845068-87845087 MS.gene054542:intron 40.0%
AGACCTAACACGGTTCGAAT+AGG - chr4.1:87845662-87845681 None:intergenic 45.0%
AGAGGAAAAGGAGCTAAAGG+TGG + chr4.1:87845280-87845299 MS.gene054542:CDS 45.0%
GACCTAACACGGTTCGAATA+GGG - chr4.1:87845661-87845680 None:intergenic 45.0%
GCTAGAGGAAAAGGAGCTAA+AGG + chr4.1:87845277-87845296 MS.gene054542:CDS 45.0%
AACCGTGTTAGGTCTGCAAG+GGG + chr4.1:87845667-87845686 MS.gene054542:CDS 50.0%
ACGGTTCGAATAGGGCTACT+CGG - chr4.1:87845653-87845672 None:intergenic 50.0%
AGCCCTATTCGAACCGTGTT+AGG + chr4.1:87845656-87845675 MS.gene054542:CDS 50.0%
GAACCGTGTTAGGTCTGCAA+GGG + chr4.1:87845666-87845685 MS.gene054542:CDS 50.0%
GCAAGGGGAGAGAGTGAATA+CGG + chr4.1:87845682-87845701 MS.gene054542:CDS 50.0%
CGAACCGTGTTAGGTCTGCA+AGG + chr4.1:87845665-87845684 MS.gene054542:CDS 55.0%
CTCCCCTTGCAGACCTAACA+CGG - chr4.1:87845672-87845691 None:intergenic 55.0%
GTAGAGCTTCGAGAACGACG+AGG - chr4.1:87845328-87845347 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87844932 87845727 87844932 ID=MS.gene054542
chr4.1 mRNA 87844932 87845727 87844932 ID=MS.gene054542.t1;Parent=MS.gene054542
chr4.1 exon 87844932 87845064 87844932 ID=MS.gene054542.t1.exon1;Parent=MS.gene054542.t1
chr4.1 CDS 87844932 87845064 87844932 ID=cds.MS.gene054542.t1;Parent=MS.gene054542.t1
chr4.1 exon 87845170 87845378 87845170 ID=MS.gene054542.t1.exon2;Parent=MS.gene054542.t1
chr4.1 CDS 87845170 87845378 87845170 ID=cds.MS.gene054542.t1;Parent=MS.gene054542.t1
chr4.1 exon 87845590 87845727 87845590 ID=MS.gene054542.t1.exon3;Parent=MS.gene054542.t1
chr4.1 CDS 87845590 87845727 87845590 ID=cds.MS.gene054542.t1;Parent=MS.gene054542.t1
Gene Sequence

>MS.gene054542

ATGAGAGTAAGATTTCTTACATGTTTCATCGCTGTTTTATGCACTCAAGCTATATCGATTTTAGGTTTCAGTTCAGAACATAGAGCTTTAAACTCGTCAAATAGCATCATGGAAACAATGCAACCTACTAGTAGTAAGCATTCTTCGCCTTTTTGAAGGAGCGTTTCCTTAAATTGTACTCATGCTATATTTAATTTCTGCTTTTAGATGCTAACTAAATTTTGCTTATTCTATTAAGATGTAGGAAACTATGGAAGCATAAAAGAAGGAAGCAAGTTGCACGGAGAAGATAAGAAACATATCAAGGAGGAAAAGTTTGAACGAGCTTATGACTCTATTAAGAAAGCTAGAGGAAAAGGAGCTAAAGGTGGTGCAAATGATAATCGCTCTGAACCTCGTCGTTCTCGAAGCTCTACATCCAAGTTATCTTGGATTTCACCTGTGTGTGTAAGCTTTGTTTTAATATCAGTCTTCTCCTTTCATGTTAAGTTGCTATGAATTTTCAAACACTTCGTGTCACATGTTTATGTTTGAAGCTTGGGATTCTTTAGAAGAGAAGATCATCTTTGTACATCATGAAACATTGTTGTCCTAATTTCTCATAACTTGAATTTAAATTTTTAACATCAATAAATGCTTCAAGTTAAGCTTGTCACAGATTTCCAAATCCAATCAGAAAGGAAAATCATCGATAGTATTATCTAAAGAGTTAATTCAACCGAGTAGCCCTATTCGAACCGTGTTAGGTCTGCAAGGGGAGAGAGTGAATACGGAGAGTGAAATCATCGATAAATAA

Protein sequence

>MS.gene054542.t1

MRVRFLTCFIAVLCTQAISILGFSSEHRALNSSNSIMETMQPTSNVGNYGSIKEGSKLHGEDKKHIKEEKFERAYDSIKKARGKGAKGGANDNRSEPRRSRSSTSKLSWISPVCISKSNQKGKSSIVLSKELIQPSSPIRTVLGLQGERVNTESEIIDK