Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene30544 | 0.819778 | 9.71E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene30544 | 0.803966 | 2.71E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene30544 | 0.82367 | 1.22E-53 | -1.69E-46 |
| MS.gene057705 | MS.gene30544 | 0.814512 | 1.48E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30544.t1 | MTR_1g115220 | 52.147 | 163 | 46 | 2 | 64 | 211 | 65 | 210 | 6.25e-30 | 110 |
| MS.gene30544.t1 | MTR_8g018100 | 53.571 | 84 | 25 | 4 | 20 | 100 | 133 | 205 | 9.27e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCTTCGCGCAGCTAAAT+TGG | 0.265099 | 4.2:-40398356 | MS.gene30544:CDS |
| TCCATGGCCTTCGTATATTC+TGG | 0.356360 | 4.2:+40397794 | None:intergenic |
| AGTATGCGACGACCGTATAA+TGG | 0.366794 | 4.2:+40398463 | None:intergenic |
| TTAGGCGAAGTATCATTCTT+TGG | 0.371753 | 4.2:-40397837 | MS.gene30544:intron |
| TCTTCCACCTCTCCTGGTGC+AGG | 0.394878 | 4.2:+40397279 | None:intergenic |
| CCGAAGGATCATCCATTATA+CGG | 0.416487 | 4.2:-40398475 | MS.gene30544:CDS |
| GTTATATAAGTACGAAGATA+TGG | 0.417339 | 4.2:-40398410 | MS.gene30544:CDS |
| TAAAATTGTTTAGGAGAAGA+AGG | 0.423795 | 4.2:-40397490 | MS.gene30544:intron |
| GGGATATGATAAGGAAGATA+AGG | 0.437427 | 4.2:-40397328 | MS.gene30544:CDS |
| CTTCCACCTCTCCTGGTGCA+GGG | 0.450689 | 4.2:+40397280 | None:intergenic |
| GTTAACTCTTCCACCTCTCC+TGG | 0.451248 | 4.2:+40397273 | None:intergenic |
| CCTTGCCGCCACCATGGACT+TGG | 0.453782 | 4.2:-40398503 | None:intergenic |
| AAAAGAACCAACTGCGAAAA+GGG | 0.458718 | 4.2:-40398160 | MS.gene30544:CDS |
| GGATCAGGAGGGATATGATA+AGG | 0.465606 | 4.2:-40397337 | MS.gene30544:CDS |
| CAAAAGAACCAACTGCGAAA+AGG | 0.465631 | 4.2:-40398161 | MS.gene30544:CDS |
| GGAAGGCCAATCTGATGATA+AGG | 0.467371 | 4.2:-40397307 | MS.gene30544:CDS |
| CCGTATAATGGATGATCCTT+CGG | 0.481434 | 4.2:+40398475 | None:intergenic |
| AGCCCCTGCACCAGGAGAGG+TGG | 0.481468 | 4.2:-40397283 | MS.gene30544:CDS |
| GGGCTTCCTTATCATCAGAT+TGG | 0.481597 | 4.2:+40397301 | None:intergenic |
| TTCCACCTCTCCTGGTGCAG+GGG | 0.483421 | 4.2:+40397281 | None:intergenic |
| GGTGGACGCTAAATTTGATC+TGG | 0.493294 | 4.2:-40398389 | MS.gene30544:CDS |
| AGTTAACAGATGAGGAGTAG+TGG | 0.515594 | 4.2:-40397257 | None:intergenic |
| GATAAGGAAGCCCCTGCACC+AGG | 0.518868 | 4.2:-40397291 | MS.gene30544:CDS |
| CTGCGAAAAGGGCTAAAGTT+GGG | 0.522171 | 4.2:-40398149 | MS.gene30544:CDS |
| TATGTTGGTTAGGAAGCTAC+TGG | 0.528713 | 4.2:-40397989 | MS.gene30544:intron |
| ACTGCGAAAAGGGCTAAAGT+TGG | 0.541934 | 4.2:-40398150 | MS.gene30544:CDS |
| GAAGCGGAGGTCTTTGACTG+AGG | 0.562628 | 4.2:-40397963 | MS.gene30544:CDS |
| GGAAGCCCCTGCACCAGGAG+AGG | 0.576902 | 4.2:-40397286 | MS.gene30544:CDS |
| GGTGGAAGAGTTAACAGATG+AGG | 0.581307 | 4.2:-40397265 | MS.gene30544:CDS |
| AAAAGGGCTAAAGTTGGGAA+AGG | 0.581404 | 4.2:-40398144 | MS.gene30544:CDS |
| GTTAGGAAGCTGCTGTGAAG+TGG | 0.586151 | 4.2:-40397623 | MS.gene30544:intron |
| TATTTATGTTTAGGATCAGG+AGG | 0.589442 | 4.2:-40397349 | MS.gene30544:intron |
| AGCAGCAGCATACAATTCCA+TGG | 0.590575 | 4.2:+40397778 | None:intergenic |
| CTTCGGAAACTCCAAGTCCA+TGG | 0.591118 | 4.2:+40398492 | None:intergenic |
| CATGGACTTGGAGTTTCCGA+AGG | 0.595740 | 4.2:-40398491 | MS.gene30544:CDS |
| GCCAGAATATACGAAGGCCA+TGG | 0.602963 | 4.2:-40397795 | MS.gene30544:CDS |
| AAGCTACTGGTGAGAAGCGG+AGG | 0.606898 | 4.2:-40397976 | MS.gene30544:CDS |
| GGCAGCATTATACAAATGCA+TGG | 0.610143 | 4.2:+40397467 | None:intergenic |
| ATTTATGTTTAGGATCAGGA+GGG | 0.610871 | 4.2:-40397348 | MS.gene30544:intron |
| GGAAGCTGCTGTGAAGTGGA+TGG | 0.615800 | 4.2:-40397619 | MS.gene30544:CDS |
| AAAGGGCTAAAGTTGGGAAA+GGG | 0.618574 | 4.2:-40398143 | MS.gene30544:CDS |
| TATGATAAGGAAGATAAGGA+AGG | 0.621270 | 4.2:-40397324 | MS.gene30544:CDS |
| GAAGTGGATGGCTTTGATTG+AGG | 0.630707 | 4.2:-40397607 | MS.gene30544:CDS |
| AGGAAGCTACTGGTGAGAAG+CGG | 0.661605 | 4.2:-40397979 | MS.gene30544:CDS |
| CTGAAGTGAAGAAACCTGAG+AGG | 0.670696 | 4.2:-40398291 | MS.gene30544:intron |
| ATATAAGTACGAAGATATGG+TGG | 0.694754 | 4.2:-40398407 | MS.gene30544:CDS |
| ACGCAGGCCAGAATATACGA+AGG | 0.729822 | 4.2:-40397801 | MS.gene30544:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAGGTTTATTTATTTTTTT+TGG | - | chr4.2:40397542-40397561 | MS.gene30544:intron | 10.0% |
| !! | ATGTATTGAAAAAATTATGT+TGG | - | chr4.2:40397746-40397765 | MS.gene30544:intron | 15.0% |
| !!! | TATTTTTTTTGGAATTTTGT+AGG | - | chr4.2:40397553-40397572 | MS.gene30544:intron | 15.0% |
| !! | AATATTCACAGACTAAAATT+GGG | + | chr4.2:40397497-40397516 | None:intergenic | 20.0% |
| !! | ATGCATTGAAAAAATTATGT+TGG | - | chr4.2:40398105-40398124 | MS.gene30544:intron | 20.0% |
| !! | TGATATGGTTAAAATTGTTT+AGG | - | chr4.2:40397895-40397914 | MS.gene30544:intron | 20.0% |
| !! | TTGTTTGATTAACTTATCTA+AGG | - | chr4.2:40397702-40397721 | MS.gene30544:intron | 20.0% |
| !!! | AAATGAAGTATGTTTTCTTT+TGG | - | chr4.2:40397656-40397675 | MS.gene30544:intron | 20.0% |
| !!! | AATGAAGTATGTTTTCTTTT+GGG | - | chr4.2:40397657-40397676 | MS.gene30544:intron | 20.0% |
| !!! | ATGTTTTGTTCTGTTTTTAT+TGG | - | chr4.2:40397827-40397846 | MS.gene30544:CDS | 20.0% |
| !!! | TTGAAAAAATTATGTTGGTT+AGG | - | chr4.2:40397751-40397770 | MS.gene30544:CDS | 20.0% |
| ! | ATATTCACAGACTAAAATTG+GGG | + | chr4.2:40397496-40397515 | None:intergenic | 25.0% |
| ! | CAATATTCACAGACTAAAAT+TGG | + | chr4.2:40397498-40397517 | None:intergenic | 25.0% |
| ! | GTAAGTTTGATTACAATGTT+TGG | - | chr4.2:40398168-40398187 | MS.gene30544:CDS | 25.0% |
| ! | GTTATATAAGTACGAAGATA+TGG | - | chr4.2:40397340-40397359 | MS.gene30544:intron | 25.0% |
| ! | TAAAATTGTTTAGGAGAAGA+AGG | - | chr4.2:40398260-40398279 | MS.gene30544:intron | 25.0% |
| !! | AAATGAAGTATGTTTTCTTC+TGG | - | chr4.2:40398314-40398333 | MS.gene30544:CDS | 25.0% |
| !! | AATGAAGTATGTTTTCTTCT+GGG | - | chr4.2:40398315-40398334 | MS.gene30544:CDS | 25.0% |
| !! | CTATATTTATGTTTAGGATC+AGG | - | chr4.2:40398398-40398417 | MS.gene30544:CDS | 25.0% |
| !! | GCTAAAGTATGTTTTCTTTT+GGG | - | chr4.2:40398016-40398035 | MS.gene30544:intron | 25.0% |
| !! | TGCTAAAGTATGTTTTCTTT+TGG | - | chr4.2:40398015-40398034 | MS.gene30544:intron | 25.0% |
| !! | TTTTAGTCTGTGAATATTGT+TGG | - | chr4.2:40397499-40397518 | MS.gene30544:intron | 25.0% |
| !!! | TCTGGTTTTTGTTAGTATTT+AGG | - | chr4.2:40397524-40397543 | MS.gene30544:intron | 25.0% |
| !!! | TGTTCTGTTTTTATTGGATT+TGG | - | chr4.2:40397833-40397852 | MS.gene30544:CDS | 25.0% |
| ATATAAGTACGAAGATATGG+TGG | - | chr4.2:40397343-40397362 | MS.gene30544:intron | 30.0% | |
| TATGATAAGGAAGATAAGGA+AGG | - | chr4.2:40398426-40398445 | MS.gene30544:CDS | 30.0% | |
| TATTCACAGACTAAAATTGG+GGG | + | chr4.2:40397495-40397514 | None:intergenic | 30.0% | |
| TTTATGTGATGCTGATCTAA+TGG | - | chr4.2:40397870-40397889 | MS.gene30544:intron | 30.0% | |
| ! | ATTTATGTTTAGGATCAGGA+GGG | - | chr4.2:40398402-40398421 | MS.gene30544:CDS | 30.0% |
| ! | TATTTATGTTTAGGATCAGG+AGG | - | chr4.2:40398401-40398420 | MS.gene30544:CDS | 30.0% |
| !! | CTGGTGCTATATTTATGTTT+AGG | - | chr4.2:40398392-40398411 | MS.gene30544:CDS | 30.0% |
| !!! | ATGTTTGGTTCTGTTTTGAT+TGG | - | chr4.2:40398183-40398202 | MS.gene30544:intron | 30.0% |
| !!! | TCGCATACTAAAGCTTTTTT+CGG | - | chr4.2:40397301-40397320 | MS.gene30544:CDS | 30.0% |
| AAAAGAACCAACTGCGAAAA+GGG | - | chr4.2:40397590-40397609 | MS.gene30544:intron | 35.0% | |
| GCTGATCTAATGGTATGATA+TGG | - | chr4.2:40397880-40397899 | MS.gene30544:intron | 35.0% | |
| GGGATATGATAAGGAAGATA+AGG | - | chr4.2:40398422-40398441 | MS.gene30544:CDS | 35.0% | |
| ! | ACATACTTCATTTTCAGCAG+TGG | + | chr4.2:40398307-40398326 | None:intergenic | 35.0% |
| ! | TTAGGCGAAGTATCATTCTT+TGG | - | chr4.2:40397913-40397932 | MS.gene30544:intron | 35.0% |
| !! | GCAGTTGGTTCTTTTGAATT+TGG | + | chr4.2:40397585-40397604 | None:intergenic | 35.0% |
| !!! | GGTTCTGTTTTGATTGGATT+TGG | - | chr4.2:40398189-40398208 | MS.gene30544:intron | 35.0% |
| AAAAGGGCTAAAGTTGGGAA+AGG | - | chr4.2:40397606-40397625 | MS.gene30544:CDS | 40.0% | |
| AAAGGGCTAAAGTTGGGAAA+GGG | - | chr4.2:40397607-40397626 | MS.gene30544:CDS | 40.0% | |
| CAAAAGAACCAACTGCGAAA+AGG | - | chr4.2:40397589-40397608 | MS.gene30544:intron | 40.0% | |
| CCGAAGGATCATCCATTATA+CGG | - | chr4.2:40397275-40397294 | MS.gene30544:CDS | 40.0% | |
| CTGTGAATATTGTTGGACTC+TGG | - | chr4.2:40397506-40397525 | MS.gene30544:intron | 40.0% | |
| GGCAGCATTATACAAATGCA+TGG | + | chr4.2:40398286-40398305 | None:intergenic | 40.0% | |
| GGGCTTCTATGAATTGTGTT+TGG | - | chr4.2:40397677-40397696 | MS.gene30544:intron | 40.0% | |
| ! | TATGTTGGTTAGGAAGCTAC+TGG | - | chr4.2:40397761-40397780 | MS.gene30544:CDS | 40.0% |
| ! | TGGTTAGAGTTTTTGAACGC+AGG | - | chr4.2:40397933-40397952 | MS.gene30544:intron | 40.0% |
| !! | CCGTATAATGGATGATCCTT+CGG | + | chr4.2:40397278-40397297 | None:intergenic | 40.0% |
| !!! | ACTAAAGCTTTTTTCGGACG+AGG | - | chr4.2:40397307-40397326 | MS.gene30544:CDS | 40.0% |
| !!! | TTTGCTCTCTTTTCTGAAGC+TGG | - | chr4.2:40398373-40398392 | MS.gene30544:CDS | 40.0% |
| ACTGCGAAAAGGGCTAAAGT+TGG | - | chr4.2:40397600-40397619 | MS.gene30544:intron | 45.0% | |
| AGCAGCAGCATACAATTCCA+TGG | + | chr4.2:40397975-40397994 | None:intergenic | 45.0% | |
| AGTATGCGACGACCGTATAA+TGG | + | chr4.2:40397290-40397309 | None:intergenic | 45.0% | |
| CTGAAGTGAAGAAACCTGAG+AGG | - | chr4.2:40397459-40397478 | MS.gene30544:CDS | 45.0% | |
| CTGCGAAAAGGGCTAAAGTT+GGG | - | chr4.2:40397601-40397620 | MS.gene30544:intron | 45.0% | |
| GGAAGGCCAATCTGATGATA+AGG | - | chr4.2:40398443-40398462 | MS.gene30544:CDS | 45.0% | |
| GGATCAGGAGGGATATGATA+AGG | - | chr4.2:40398413-40398432 | MS.gene30544:CDS | 45.0% | |
| GGGCTTCCTTATCATCAGAT+TGG | + | chr4.2:40398452-40398471 | None:intergenic | 45.0% | |
| GGGGAAAAATTTCACCTCTC+AGG | + | chr4.2:40397476-40397495 | None:intergenic | 45.0% | |
| GGTGGAAGAGTTAACAGATG+AGG | - | chr4.2:40398485-40398504 | MS.gene30544:CDS | 45.0% | |
| GGTGGACGCTAAATTTGATC+TGG | - | chr4.2:40397361-40397380 | MS.gene30544:intron | 45.0% | |
| TCCATGGCCTTCGTATATTC+TGG | + | chr4.2:40397959-40397978 | None:intergenic | 45.0% | |
| ! | ACTTTAGCCCTTTTCGCAGT+TGG | + | chr4.2:40397600-40397619 | None:intergenic | 45.0% |
| !! | GAAGTGGATGGCTTTGATTG+AGG | - | chr4.2:40398143-40398162 | MS.gene30544:CDS | 45.0% |
| ACGCAGGCCAGAATATACGA+AGG | - | chr4.2:40397949-40397968 | MS.gene30544:intron | 50.0% | |
| AGAGCTTCGCGCAGCTAAAT+TGG | - | chr4.2:40397394-40397413 | MS.gene30544:intron | 50.0% | |
| AGGAAGCTACTGGTGAGAAG+CGG | - | chr4.2:40397771-40397790 | MS.gene30544:CDS | 50.0% | |
| GCCAGAATATACGAAGGCCA+TGG | - | chr4.2:40397955-40397974 | MS.gene30544:intron | 50.0% | |
| GTTAACTCTTCCACCTCTCC+TGG | + | chr4.2:40398480-40398499 | None:intergenic | 50.0% | |
| GTTAGGAAGCTGCTGTGAAG+TGG | - | chr4.2:40398127-40398146 | MS.gene30544:CDS | 50.0% | |
| !! | TTTTTCGGACGAGGCACTCT+TGG | - | chr4.2:40397316-40397335 | MS.gene30544:CDS | 50.0% |
| AAGCTACTGGTGAGAAGCGG+AGG | - | chr4.2:40397774-40397793 | MS.gene30544:CDS | 55.0% | |
| GAAGCGGAGGTCTTTGACTG+AGG | - | chr4.2:40397787-40397806 | MS.gene30544:CDS | 55.0% | |
| GGAAGCTGCTGTGAAGTGGA+TGG | - | chr4.2:40398131-40398150 | MS.gene30544:CDS | 55.0% | |
| CTTCCACCTCTCCTGGTGCA+GGG | + | chr4.2:40398473-40398492 | None:intergenic | 60.0% | |
| GATAAGGAAGCCCCTGCACC+AGG | - | chr4.2:40398459-40398478 | MS.gene30544:CDS | 60.0% | |
| TCTTCCACCTCTCCTGGTGC+AGG | + | chr4.2:40398474-40398493 | None:intergenic | 60.0% | |
| TTCCACCTCTCCTGGTGCAG+GGG | + | chr4.2:40398472-40398491 | None:intergenic | 60.0% | |
| AGCCCCTGCACCAGGAGAGG+TGG | - | chr4.2:40398467-40398486 | MS.gene30544:CDS | 70.0% | |
| GGAAGCCCCTGCACCAGGAG+AGG | - | chr4.2:40398464-40398483 | MS.gene30544:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 40397260 | 40398512 | 40397260 | ID=MS.gene30544 |
| chr4.2 | mRNA | 40397260 | 40398512 | 40397260 | ID=MS.gene30544.t1;Parent=MS.gene30544 |
| chr4.2 | exon | 40398292 | 40398512 | 40398292 | ID=MS.gene30544.t1.exon1;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40398292 | 40398512 | 40398292 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40398110 | 40398197 | 40398110 | ID=MS.gene30544.t1.exon2;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40398110 | 40398197 | 40398110 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40397961 | 40397999 | 40397961 | ID=MS.gene30544.t1.exon3;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40397961 | 40397999 | 40397961 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40397751 | 40397855 | 40397751 | ID=MS.gene30544.t1.exon4;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40397751 | 40397855 | 40397751 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40397605 | 40397640 | 40397605 | ID=MS.gene30544.t1.exon5;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40397605 | 40397640 | 40397605 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40397452 | 40397499 | 40397452 | ID=MS.gene30544.t1.exon6;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40397452 | 40397499 | 40397452 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| chr4.2 | exon | 40397260 | 40397358 | 40397260 | ID=MS.gene30544.t1.exon7;Parent=MS.gene30544.t1 |
| chr4.2 | CDS | 40397260 | 40397358 | 40397260 | ID=cds.MS.gene30544.t1;Parent=MS.gene30544.t1 |
| Gene Sequence |
| Protein sequence |
