AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30690.t1 MTR_6g048300 95.184 353 8 3 1 348 1 349 0.0 665
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30690.t1 AT5G24020 80.368 326 39 2 24 348 25 326 3.21e-176 492

Find 130 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGAGTGTGATGGGATT+AGG 0.203845 6.3:-43424133 MS.gene30690:CDS
GCCTCCTACTTTGGCTGGTT+TGG 0.227893 6.3:-43423940 MS.gene30690:CDS
CGAAGATATGATGTCGGTTT+TGG 0.228805 6.3:-43424063 MS.gene30690:CDS
CGCCGGATATAACTGCATTA+AGG 0.237827 6.3:-43424178 MS.gene30690:CDS
GGGTTTGGGTTTGGGGAATG+TGG 0.249661 6.3:+43424778 None:intergenic
CCAATAGGTAATTTAGATCT+AGG 0.260628 6.3:+43424425 None:intergenic
CCTAGATCTAAATTACCTAT+TGG 0.278293 6.3:-43424425 MS.gene30690:CDS
TGGGGTTTCGCCGGAGAGTT+GGG 0.291059 6.3:+43424712 None:intergenic
TTGAGATTGGTGGTGGTGCC+GGG 0.299756 6.3:+43424839 None:intergenic
TCTAAATTACCTATTGGATT+TGG 0.301556 6.3:-43424419 MS.gene30690:CDS
AACTCCAGTTCCACTGGTTC+TGG 0.323824 6.3:-43424296 MS.gene30690:CDS
GTTGGAGTGTGATGGGATTA+GGG 0.327318 6.3:-43424132 MS.gene30690:CDS
GGGTTTGGGGAATGTGGGAG+AGG 0.332273 6.3:+43424784 None:intergenic
TTTGAGATTGGTGGTGGTGC+CGG 0.343813 6.3:+43424838 None:intergenic
CGGAGGATAGTGAGGTTATA+AGG 0.346345 6.3:-43423998 MS.gene30690:CDS
CGCCGGAGAGTTGGGGTTTG+CGG 0.359616 6.3:+43424720 None:intergenic
GGATAGTATGCAAGCTGTTA+TGG 0.362457 6.3:-43423883 MS.gene30690:CDS
GTGGGGTTTCGCCGGAGAGT+TGG 0.366229 6.3:+43424711 None:intergenic
ACTGGAACCAATGGTGGTTC+TGG 0.373685 6.3:-43424323 MS.gene30690:CDS
GCTAGGACGGATATGATTAA+AGG 0.384292 6.3:-43424086 MS.gene30690:CDS
TTTGAATAAGCCTCCTACTT+TGG 0.386067 6.3:-43423949 MS.gene30690:CDS
TGCCCAGCGGGGATCGATGC+AGG 0.386578 6.3:-43424248 MS.gene30690:CDS
AATAAGCCTCCTACTTTGGC+TGG 0.388357 6.3:-43423945 MS.gene30690:CDS
GGTTTGGGTTTGGGGAATGT+GGG 0.391660 6.3:+43424779 None:intergenic
ATATTAAGATGATAGTGAAT+AGG 0.397168 6.3:-43424109 MS.gene30690:CDS
CAACAAAATCCCTTCTTCAA+TGG 0.399116 6.3:-43424745 MS.gene30690:CDS
GGAGATAAAACCGGTGGAAA+TGG 0.404981 6.3:-43424353 MS.gene30690:CDS
ATCAAAACCGCTTCATTAGC+AGG 0.415640 6.3:+43424206 None:intergenic
CGACGACGGAGAAGCCTAAA+CGG 0.416599 6.3:+43424612 None:intergenic
GCCGTTGCTTGGTGTTATAC+CGG 0.418035 6.3:-43424018 MS.gene30690:CDS
TATTAAGATGATAGTGAATA+GGG 0.421269 6.3:-43424108 MS.gene30690:CDS
AGGGAAGTGGGATTTGAGAT+TGG 0.424623 6.3:+43424826 None:intergenic
AAATTACCTATTGGATTTGG+TGG 0.424983 6.3:-43424416 MS.gene30690:CDS
TTCCACTGGTTCTGGCTGTC+CGG 0.425939 6.3:-43424288 MS.gene30690:CDS
GGATGGAGATGGGTGATTGA+AGG 0.430261 6.3:+43424806 None:intergenic
ACAGCCAGAACCAGTGGAAC+TGG 0.431566 6.3:+43424292 None:intergenic
CAAGACCTAGTAAAAGGTCT+AGG 0.433285 6.3:+43424561 None:intergenic
GCCGGATATAACTGCATTAA+GGG 0.435899 6.3:-43424177 MS.gene30690:CDS
TTGGATGTGCAAGAGATGTT+GGG 0.436831 6.3:-43424044 MS.gene30690:CDS
TCCCCGCTGGGCAATCGATC+AGG 0.438912 6.3:+43424258 None:intergenic
GGTTCCAACTCCAGTTCCAC+TGG 0.439282 6.3:-43424302 MS.gene30690:CDS
GGTTTGGGGAATGTGGGAGA+GGG 0.445213 6.3:+43424785 None:intergenic
GTGGGATTTGAGATTGGTGG+TGG 0.456536 6.3:+43424832 None:intergenic
AAAACCGGTGGAAATGGAAA+TGG 0.458507 6.3:-43424347 MS.gene30690:CDS
GGTGGAAATGGAAATGGAAC+TGG 0.459176 6.3:-43424341 MS.gene30690:CDS
TCCCTTAATGCAGTTATATC+CGG 0.461447 6.3:+43424176 None:intergenic
GATGGAGATGGGTGATTGAA+GGG 0.461891 6.3:+43424807 None:intergenic
TGCTTGGAGACTTGTGGAGC+AGG 0.462222 6.3:-43423904 MS.gene30690:CDS
AACCCTGCATCGATCCCCGC+TGG 0.463979 6.3:+43424245 None:intergenic
ATCCTGATCGATTGCCCAGC+GGG 0.465074 6.3:-43424260 MS.gene30690:CDS
GTAGTAGTCACATCCGGAAA+AGG 0.466734 6.3:-43424683 MS.gene30690:CDS
ATGTGGGAGAGGGATGGAGA+TGG 0.467686 6.3:+43424795 None:intergenic
TGTGGGAGAGGGATGGAGAT+GGG 0.471520 6.3:+43424796 None:intergenic
CGGGATTGTTGGAGTGTGAT+GGG 0.471806 6.3:-43424139 MS.gene30690:CDS
CGCTTCATTAGCAGGCGTAA+TGG 0.474476 6.3:+43424214 None:intergenic
TTGTCGACTCGATCAAGCTT+TGG 0.474531 6.3:-43424495 MS.gene30690:CDS
TGGTGCCGGGGTGGTTGTAG+AGG 0.476793 6.3:+43424852 None:intergenic
ATGGGTGATTGAAGGGAAGT+GGG 0.477183 6.3:+43424814 None:intergenic
TTTGGATGTGCAAGAGATGT+TGG 0.492861 6.3:-43424045 MS.gene30690:CDS
AGATAGAGTGACGGGATTGT+TGG 0.502060 6.3:-43424150 MS.gene30690:CDS
GTTTGCGGTTCCATTGAAGA+AGG 0.502690 6.3:+43424735 None:intergenic
TGGAAAAGCTTTGACTTGGT+TGG 0.503051 6.3:-43424396 MS.gene30690:CDS
AGAACCAGTGGAACTGGAGT+TGG 0.505336 6.3:+43424298 None:intergenic
GACGACGGAGAAGCCTAAAC+GGG 0.508282 6.3:+43424613 None:intergenic
TACAACGCGTGGGGTTTCGC+CGG 0.515932 6.3:+43424703 None:intergenic
TGGTTGAAGCTTTAAAAGCC+CGG 0.516057 6.3:-43424376 MS.gene30690:CDS
ACGGGATTGTTGGAGTGTGA+TGG 0.518610 6.3:-43424140 MS.gene30690:CDS
AACCGCAAACCCCAACTCTC+CGG 0.519734 6.3:-43424722 MS.gene30690:CDS
ATGTTGGGTTTGCCGTTGCT+TGG 0.520733 6.3:-43424029 MS.gene30690:CDS
GGCCTTTCTTTAGCCCGTTT+AGG 0.522513 6.3:-43424626 MS.gene30690:CDS
GATGGGTGATTGAAGGGAAG+TGG 0.523627 6.3:+43424813 None:intergenic
TGGGGAATGTGGGAGAGGGA+TGG 0.524913 6.3:+43424789 None:intergenic
GGTTGAAGCTTTAAAAGCCC+GGG 0.525956 6.3:-43424375 MS.gene30690:CDS
AGCCTAAACGGGCTAAAGAA+AGG 0.528847 6.3:+43424624 None:intergenic
AGATGATAGTGAATAGGGCT+AGG 0.530504 6.3:-43424103 MS.gene30690:CDS
AGTTCCATTTCCATTTCCAC+CGG 0.532503 6.3:+43424343 None:intergenic
TATGCAAGCTGTTATGGTTG+AGG 0.533160 6.3:-43423877 MS.gene30690:CDS
CGCGTTGTAGTAGTCACATC+CGG 0.533985 6.3:-43424689 MS.gene30690:CDS
AAAGCCCGGGGAGATAAAAC+CGG 0.540961 6.3:-43424362 MS.gene30690:CDS
GCCCAGCGGGGATCGATGCA+GGG 0.541734 6.3:-43424247 MS.gene30690:CDS
GCCAAACCAGCCAAAGTAGG+AGG 0.542195 6.3:+43423939 None:intergenic
ACATCCGGAAAAGGCGGAGT+TGG 0.544305 6.3:-43424674 MS.gene30690:CDS
GCCGATGTTAGCGGTTGTAG+TGG 0.546601 6.3:+43424646 None:intergenic
TAATCCGACGTCGGCGTCGA+TGG 0.548038 6.3:+43424589 None:intergenic
AAAGCCAAACCAGCCAAAGT+AGG 0.548994 6.3:+43423936 None:intergenic
GTTGGAACCAGAACCACCAT+TGG 0.552319 6.3:+43424316 None:intergenic
TGAGATTGGTGGTGGTGCCG+GGG 0.553356 6.3:+43424840 None:intergenic
ACAAGCTGCTTGGAGACTTG+TGG 0.556461 6.3:-43423910 MS.gene30690:CDS
GGTGCCGGGGTGGTTGTAGA+GGG 0.557844 6.3:+43424853 None:intergenic
AGGGATGCAGATAGAGTGAC+GGG 0.560584 6.3:-43424158 MS.gene30690:CDS
TAAAGAAAGGCCGATGTTAG+CGG 0.565526 6.3:+43424637 None:intergenic
GATAGTGAATAGGGCTAGGA+CGG 0.567848 6.3:-43424099 MS.gene30690:CDS
CAATCGATCAGGATAAAGTC+CGG 0.577067 6.3:+43424269 None:intergenic
GTCCGGACAGCCAGAACCAG+TGG 0.577561 6.3:+43424286 None:intergenic
CTTATAACCTCACTATCCTC+CGG 0.578009 6.3:+43423999 None:intergenic
GATTGGTGGTGGTGCCGGGG+TGG 0.578938 6.3:+43424843 None:intergenic
GGAGGCTTATTCAAAACAAG+TGG 0.583747 6.3:+43423957 None:intergenic
GTTGAGGAAGAGCCTAAAAG+AGG 0.586290 6.3:-43423861 MS.gene30690:CDS
GTCGCCATCGACGCCGACGT+CGG 0.588739 6.3:-43424593 MS.gene30690:CDS
TTTGCGGTTCCATTGAAGAA+GGG 0.588939 6.3:+43424736 None:intergenic
GGGGTTTCGCCGGAGAGTTG+GGG 0.590473 6.3:+43424713 None:intergenic
GTCGGCGTCGATGGCGACGA+CGG 0.595379 6.3:+43424598 None:intergenic
TTGGTGGAAAAGCTTTGACT+TGG 0.597183 6.3:-43424400 MS.gene30690:CDS
ACCACTACAACCGCTAACAT+CGG 0.600288 6.3:-43424647 MS.gene30690:CDS
ACCCTGCATCGATCCCCGCT+GGG 0.604891 6.3:+43424246 None:intergenic
GATGTGACTACTACAACGCG+TGG 0.607370 6.3:+43424692 None:intergenic
CATTACGCCTGCTAATGAAG+CGG 0.609060 6.3:-43424213 MS.gene30690:CDS
AAGGGATGCAGATAGAGTGA+CGG 0.617712 6.3:-43424159 MS.gene30690:CDS
GGAAATGGAACTGGAACCAA+TGG 0.618971 6.3:-43424332 MS.gene30690:CDS
GTTGCTTGGTGTTATACCGG+AGG 0.625941 6.3:-43424015 MS.gene30690:CDS
CTTTGGTTAGAGATAAACGT+TGG 0.628186 6.3:-43424478 MS.gene30690:CDS
ATGTGACTACTACAACGCGT+GGG 0.629497 6.3:+43424693 None:intergenic
TAAAGGCGAAGATATGATGT+CGG 0.631412 6.3:-43424069 MS.gene30690:CDS
TTATAAGGAGTACGAATCGA+GGG 0.635032 6.3:-43423983 MS.gene30690:CDS
GCCCGGGGAGATAAAACCGG+TGG 0.644531 6.3:-43424359 MS.gene30690:CDS
GAAGTGGGATTTGAGATTGG+TGG 0.644997 6.3:+43424829 None:intergenic
TGTTATACCGGAGGATAGTG+AGG 0.645901 6.3:-43424006 MS.gene30690:CDS
GTTATAAGGAGTACGAATCG+AGG 0.649607 6.3:-43423984 MS.gene30690:CDS
AATGGAACTGGAACCAATGG+TGG 0.654352 6.3:-43424329 MS.gene30690:CDS
GTAGTCACATCCGGAAAAGG+CGG 0.654517 6.3:-43424680 MS.gene30690:CDS
TTGAGGAAGAGCCTAAAAGA+GGG 0.658061 6.3:-43423860 MS.gene30690:CDS
TCCGGTATAACACCAAGCAA+CGG 0.658522 6.3:+43424017 None:intergenic
TGTGACTACTACAACGCGTG+GGG 0.658750 6.3:+43424694 None:intergenic
TCCTGATCGATTGCCCAGCG+GGG 0.660033 6.3:-43424259 MS.gene30690:CDS
GTTGAAGCTTTAAAAGCCCG+GGG 0.666393 6.3:-43424374 MS.gene30690:CDS
ACCGTCGTTGAGATTCTCAA+CGG 0.685318 6.3:-43424521 MS.gene30690:CDS
TATCCTGATCGATTGCCCAG+CGG 0.703174 6.3:-43424261 MS.gene30690:CDS
GCCGTTGAGAATCTCAACGA+CGG 0.724103 6.3:+43424520 None:intergenic
TAGGTTACGTAATCCGACGT+CGG 0.748882 6.3:+43424580 None:intergenic
CTATCCCTCTACAACCACCC+CGG 0.752007 6.3:-43424857 MS.gene30690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTAAGATGATAGTGAAT+AGG - chr6.3:43424587-43424606 MS.gene30690:CDS 20.0%
!!! TATTAAGATGATAGTGAATA+GGG - chr6.3:43424588-43424607 MS.gene30690:CDS 20.0%
! TCTAAATTACCTATTGGATT+TGG - chr6.3:43424277-43424296 MS.gene30690:CDS 25.0%
AAATTACCTATTGGATTTGG+TGG - chr6.3:43424280-43424299 MS.gene30690:CDS 30.0%
CCAATAGGTAATTTAGATCT+AGG + chr6.3:43424274-43424293 None:intergenic 30.0%
CCTAGATCTAAATTACCTAT+TGG - chr6.3:43424271-43424290 MS.gene30690:CDS 30.0%
!! GATTTTCAAGACCTAGTAAA+AGG + chr6.3:43424144-43424163 None:intergenic 30.0%
!!! AAGAAGGGATTTTGTTGTTA+GGG + chr6.3:43423948-43423967 None:intergenic 30.0%
!!! GATTTTGTTGTTAGGGTTTT+GGG + chr6.3:43423941-43423960 None:intergenic 30.0%
CAACAAAATCCCTTCTTCAA+TGG - chr6.3:43423951-43423970 MS.gene30690:CDS 35.0%
CTTTGGTTAGAGATAAACGT+TGG - chr6.3:43424218-43424237 MS.gene30690:CDS 35.0%
TAAAGGCGAAGATATGATGT+CGG - chr6.3:43424627-43424646 MS.gene30690:CDS 35.0%
TCCCTTAATGCAGTTATATC+CGG + chr6.3:43424523-43424542 None:intergenic 35.0%
TTTGAATAAGCCTCCTACTT+TGG - chr6.3:43424747-43424766 MS.gene30690:CDS 35.0%
! AACGAGAAAAACCCTCTTTT+AGG + chr6.3:43424850-43424869 None:intergenic 35.0%
! TTATAAGGAGTACGAATCGA+GGG - chr6.3:43424713-43424732 MS.gene30690:CDS 35.0%
!! AGAGGGTTTTTCTCGTTTTT+TGG - chr6.3:43424853-43424872 MS.gene30690:CDS 35.0%
!!! GAAGAAGGGATTTTGTTGTT+AGG + chr6.3:43423949-43423968 None:intergenic 35.0%
!!! GGATTTTGTTGTTAGGGTTT+TGG + chr6.3:43423942-43423961 None:intergenic 35.0%
AAAACCGGTGGAAATGGAAA+TGG - chr6.3:43424349-43424368 MS.gene30690:CDS 40.0%
AGTTCCATTTCCATTTCCAC+CGG + chr6.3:43424356-43424375 None:intergenic 40.0%
ATCAAAACCGCTTCATTAGC+AGG + chr6.3:43424493-43424512 None:intergenic 40.0%
CAAGACCTAGTAAAAGGTCT+AGG + chr6.3:43424138-43424157 None:intergenic 40.0%
CAATCGATCAGGATAAAGTC+CGG + chr6.3:43424430-43424449 None:intergenic 40.0%
CTTATAACCTCACTATCCTC+CGG + chr6.3:43424700-43424719 None:intergenic 40.0%
GCCGGATATAACTGCATTAA+GGG - chr6.3:43424519-43424538 MS.gene30690:CDS 40.0%
GCTAGGACGGATATGATTAA+AGG - chr6.3:43424610-43424629 MS.gene30690:CDS 40.0%
GGAGGCTTATTCAAAACAAG+TGG + chr6.3:43424742-43424761 None:intergenic 40.0%
GGATAGTATGCAAGCTGTTA+TGG - chr6.3:43424813-43424832 MS.gene30690:CDS 40.0%
TAAAGAAAGGCCGATGTTAG+CGG + chr6.3:43424062-43424081 None:intergenic 40.0%
TATGCAAGCTGTTATGGTTG+AGG - chr6.3:43424819-43424838 MS.gene30690:CDS 40.0%
TTGAGGAAGAGCCTAAAAGA+GGG - chr6.3:43424836-43424855 MS.gene30690:CDS 40.0%
TTGGATGTGCAAGAGATGTT+GGG - chr6.3:43424652-43424671 MS.gene30690:CDS 40.0%
TTTGCGGTTCCATTGAAGAA+GGG + chr6.3:43423963-43423982 None:intergenic 40.0%
TTTGGATGTGCAAGAGATGT+TGG - chr6.3:43424651-43424670 MS.gene30690:CDS 40.0%
! AGATGATAGTGAATAGGGCT+AGG - chr6.3:43424593-43424612 MS.gene30690:CDS 40.0%
! CGTAACCTAGACCTTTTACT+AGG - chr6.3:43424130-43424149 MS.gene30690:CDS 40.0%
! GCTTTTCCACCAAATCCAAT+AGG + chr6.3:43424289-43424308 None:intergenic 40.0%
! GTTATAAGGAGTACGAATCG+AGG - chr6.3:43424712-43424731 MS.gene30690:CDS 40.0%
! TTGGTGGAAAAGCTTTGACT+TGG - chr6.3:43424296-43424315 MS.gene30690:CDS 40.0%
!! TGGAAAAGCTTTGACTTGGT+TGG - chr6.3:43424300-43424319 MS.gene30690:CDS 40.0%
!! TGGTTGAAGCTTTAAAAGCC+CGG - chr6.3:43424320-43424339 MS.gene30690:CDS 40.0%
!!! CGAAGATATGATGTCGGTTT+TGG - chr6.3:43424633-43424652 MS.gene30690:CDS 40.0%
!!! GGGTTTTTCTCGTTTTTTGG+TGG - chr6.3:43424856-43424875 MS.gene30690:CDS 40.0%
!!! GTTGTTAGGGTTTTGGGTTT+GGG + chr6.3:43423935-43423954 None:intergenic 40.0%
!!! TGTTGTTAGGGTTTTGGGTT+TGG + chr6.3:43423936-43423955 None:intergenic 40.0%
AAAGCCAAACCAGCCAAAGT+AGG + chr6.3:43424763-43424782 None:intergenic 45.0%
AAGGGATGCAGATAGAGTGA+CGG - chr6.3:43424537-43424556 MS.gene30690:CDS 45.0%
AATAAGCCTCCTACTTTGGC+TGG - chr6.3:43424751-43424770 MS.gene30690:CDS 45.0%
AATGGAACTGGAACCAATGG+TGG - chr6.3:43424367-43424386 MS.gene30690:CDS 45.0%
ACCACTACAACCGCTAACAT+CGG - chr6.3:43424049-43424068 MS.gene30690:CDS 45.0%
ACCGTCGTTGAGATTCTCAA+CGG - chr6.3:43424175-43424194 MS.gene30690:CDS 45.0%
ATGGGTGATTGAAGGGAAGT+GGG + chr6.3:43423885-43423904 None:intergenic 45.0%
ATGTGACTACTACAACGCGT+GGG + chr6.3:43424006-43424025 None:intergenic 45.0%
CATTACGCCTGCTAATGAAG+CGG - chr6.3:43424483-43424502 MS.gene30690:CDS 45.0%
CGCCGGATATAACTGCATTA+AGG - chr6.3:43424518-43424537 MS.gene30690:CDS 45.0%
CGGAGGATAGTGAGGTTATA+AGG - chr6.3:43424698-43424717 MS.gene30690:CDS 45.0%
GATAGTGAATAGGGCTAGGA+CGG - chr6.3:43424597-43424616 MS.gene30690:CDS 45.0%
GATGGAGATGGGTGATTGAA+GGG + chr6.3:43423892-43423911 None:intergenic 45.0%
GGAAATGGAACTGGAACCAA+TGG - chr6.3:43424364-43424383 MS.gene30690:CDS 45.0%
GGAGATAAAACCGGTGGAAA+TGG - chr6.3:43424343-43424362 MS.gene30690:CDS 45.0%
GGTGGAAATGGAAATGGAAC+TGG - chr6.3:43424355-43424374 MS.gene30690:CDS 45.0%
GTAGTAGTCACATCCGGAAA+AGG - chr6.3:43424013-43424032 MS.gene30690:CDS 45.0%
GTTGAGGAAGAGCCTAAAAG+AGG - chr6.3:43424835-43424854 MS.gene30690:CDS 45.0%
GTTTGCGGTTCCATTGAAGA+AGG + chr6.3:43423964-43423983 None:intergenic 45.0%
TAGGTTACGTAATCCGACGT+CGG + chr6.3:43424119-43424138 None:intergenic 45.0%
TCCGGTATAACACCAAGCAA+CGG + chr6.3:43424682-43424701 None:intergenic 45.0%
TGTTATACCGGAGGATAGTG+AGG - chr6.3:43424690-43424709 MS.gene30690:CDS 45.0%
! AGATAGAGTGACGGGATTGT+TGG - chr6.3:43424546-43424565 MS.gene30690:CDS 45.0%
! AGGGAAGTGGGATTTGAGAT+TGG + chr6.3:43423873-43423892 None:intergenic 45.0%
! GAAGTGGGATTTGAGATTGG+TGG + chr6.3:43423870-43423889 None:intergenic 45.0%
! GTTGGAGTGTGATGGGATTA+GGG - chr6.3:43424564-43424583 MS.gene30690:CDS 45.0%
! TGGCTTTTGAACAAGCTGCT+TGG - chr6.3:43424776-43424795 MS.gene30690:CDS 45.0%
! TGTTGGAGTGTGATGGGATT+AGG - chr6.3:43424563-43424582 MS.gene30690:CDS 45.0%
! TTTACCAACTCCGCCTTTTC+CGG + chr6.3:43424029-43424048 None:intergenic 45.0%
!! AGCCTAAACGGGCTAAAGAA+AGG + chr6.3:43424075-43424094 None:intergenic 45.0%
!! AGCGGTTTTGATAACAACGC+CGG - chr6.3:43424501-43424520 MS.gene30690:CDS 45.0%
!! GGTTGAAGCTTTAAAAGCCC+GGG - chr6.3:43424321-43424340 MS.gene30690:CDS 45.0%
!! GTTGAAGCTTTAAAAGCCCG+GGG - chr6.3:43424322-43424341 MS.gene30690:CDS 45.0%
!! TTGTCGACTCGATCAAGCTT+TGG - chr6.3:43424201-43424220 MS.gene30690:CDS 45.0%
!!! AGGGTTTTGGGTTTGGGTTT+GGG + chr6.3:43423929-43423948 None:intergenic 45.0%
!!! TAGGGTTTTGGGTTTGGGTT+TGG + chr6.3:43423930-43423949 None:intergenic 45.0%
AAAGCCCGGGGAGATAAAAC+CGG - chr6.3:43424334-43424353 MS.gene30690:CDS 50.0%
ACAAGCTGCTTGGAGACTTG+TGG - chr6.3:43424786-43424805 MS.gene30690:CDS 50.0%
ACGGGATTGTTGGAGTGTGA+TGG - chr6.3:43424556-43424575 MS.gene30690:CDS 50.0%
ACTGGAACCAATGGTGGTTC+TGG - chr6.3:43424373-43424392 MS.gene30690:CDS 50.0%
AGAACCAGTGGAACTGGAGT+TGG + chr6.3:43424401-43424420 None:intergenic 50.0%
AGGGATGCAGATAGAGTGAC+GGG - chr6.3:43424538-43424557 MS.gene30690:CDS 50.0%
ATGTTGGGTTTGCCGTTGCT+TGG - chr6.3:43424667-43424686 MS.gene30690:CDS 50.0%
CGCGTTGTAGTAGTCACATC+CGG - chr6.3:43424007-43424026 MS.gene30690:CDS 50.0%
CGCTTCATTAGCAGGCGTAA+TGG + chr6.3:43424485-43424504 None:intergenic 50.0%
CGGGATTGTTGGAGTGTGAT+GGG - chr6.3:43424557-43424576 MS.gene30690:CDS 50.0%
GATGGGTGATTGAAGGGAAG+TGG + chr6.3:43423886-43423905 None:intergenic 50.0%
GATGTGACTACTACAACGCG+TGG + chr6.3:43424007-43424026 None:intergenic 50.0%
GCCGTTGAGAATCTCAACGA+CGG + chr6.3:43424179-43424198 None:intergenic 50.0%
GGATGGAGATGGGTGATTGA+AGG + chr6.3:43423893-43423912 None:intergenic 50.0%
GGCCTTTCTTTAGCCCGTTT+AGG - chr6.3:43424070-43424089 MS.gene30690:CDS 50.0%
GTAGTCACATCCGGAAAAGG+CGG - chr6.3:43424016-43424035 MS.gene30690:CDS 50.0%
GTTGGAACCAGAACCACCAT+TGG + chr6.3:43424383-43424402 None:intergenic 50.0%
TATCCTGATCGATTGCCCAG+CGG - chr6.3:43424435-43424454 MS.gene30690:CDS 50.0%
TGTGACTACTACAACGCGTG+GGG + chr6.3:43424005-43424024 None:intergenic 50.0%
! GGTTTGGGTTTGGGGAATGT+GGG + chr6.3:43423920-43423939 None:intergenic 50.0%
! GTGGGATTTGAGATTGGTGG+TGG + chr6.3:43423867-43423886 None:intergenic 50.0%
!! AACTCCAGTTCCACTGGTTC+TGG - chr6.3:43424400-43424419 MS.gene30690:CDS 50.0%
!! GCCGTTGCTTGGTGTTATAC+CGG - chr6.3:43424678-43424697 MS.gene30690:CDS 50.0%
!! GTTGCTTGGTGTTATACCGG+AGG - chr6.3:43424681-43424700 MS.gene30690:CDS 50.0%
!! TTTGAGATTGGTGGTGGTGC+CGG + chr6.3:43423861-43423880 None:intergenic 50.0%
!!! GGGTTTTGGGTTTGGGTTTG+GGG + chr6.3:43423928-43423947 None:intergenic 50.0%
!!! TTCCACCGGTTTTATCTCCC+CGG + chr6.3:43424342-43424361 None:intergenic 50.0%
AACCGCAAACCCCAACTCTC+CGG - chr6.3:43423974-43423993 MS.gene30690:CDS 55.0%
ACAGCCAGAACCAGTGGAAC+TGG + chr6.3:43424407-43424426 None:intergenic 55.0%
ACATCCGGAAAAGGCGGAGT+TGG - chr6.3:43424022-43424041 MS.gene30690:CDS 55.0%
ATCCTGATCGATTGCCCAGC+GGG - chr6.3:43424436-43424455 MS.gene30690:CDS 55.0%
ATGTGGGAGAGGGATGGAGA+TGG + chr6.3:43423904-43423923 None:intergenic 55.0%
CGACGACGGAGAAGCCTAAA+CGG + chr6.3:43424087-43424106 None:intergenic 55.0%
CTATCCCTCTACAACCACCC+CGG - chr6.3:43423839-43423858 MS.gene30690:CDS 55.0%
GACGACGGAGAAGCCTAAAC+GGG + chr6.3:43424086-43424105 None:intergenic 55.0%
GCCAAACCAGCCAAAGTAGG+AGG + chr6.3:43424760-43424779 None:intergenic 55.0%
GCCGATGTTAGCGGTTGTAG+TGG + chr6.3:43424053-43424072 None:intergenic 55.0%
GGTTCCAACTCCAGTTCCAC+TGG - chr6.3:43424394-43424413 MS.gene30690:CDS 55.0%
GGTTTGGGGAATGTGGGAGA+GGG + chr6.3:43423914-43423933 None:intergenic 55.0%
TGCTTGGAGACTTGTGGAGC+AGG - chr6.3:43424792-43424811 MS.gene30690:CDS 55.0%
TGTGGGAGAGGGATGGAGAT+GGG + chr6.3:43423903-43423922 None:intergenic 55.0%
! GCCTCCTACTTTGGCTGGTT+TGG - chr6.3:43424756-43424775 MS.gene30690:CDS 55.0%
! GGGTTTGGGTTTGGGGAATG+TGG + chr6.3:43423921-43423940 None:intergenic 55.0%
!! TTCCACTGGTTCTGGCTGTC+CGG - chr6.3:43424408-43424427 MS.gene30690:CDS 55.0%
!! TTGAGATTGGTGGTGGTGCC+GGG + chr6.3:43423860-43423879 None:intergenic 55.0%
!!! TCCACCGGTTTTATCTCCCC+GGG + chr6.3:43424341-43424360 None:intergenic 55.0%
GGGTTTGGGGAATGTGGGAG+AGG + chr6.3:43423915-43423934 None:intergenic 60.0%
TACAACGCGTGGGGTTTCGC+CGG + chr6.3:43423996-43424015 None:intergenic 60.0%
TCCTGATCGATTGCCCAGCG+GGG - chr6.3:43424437-43424456 MS.gene30690:CDS 60.0%
TGGGGAATGTGGGAGAGGGA+TGG + chr6.3:43423910-43423929 None:intergenic 60.0%
TGGGGTTTCGCCGGAGAGTT+GGG + chr6.3:43423987-43424006 None:intergenic 60.0%
! TAATCCGACGTCGGCGTCGA+TGG + chr6.3:43424110-43424129 None:intergenic 60.0%
!! TGAGATTGGTGGTGGTGCCG+GGG + chr6.3:43423859-43423878 None:intergenic 60.0%
CGCCGGAGAGTTGGGGTTTG+CGG + chr6.3:43423979-43423998 None:intergenic 65.0%
GCCCGGGGAGATAAAACCGG+TGG - chr6.3:43424337-43424356 MS.gene30690:CDS 65.0%
GGGGTTTCGCCGGAGAGTTG+GGG + chr6.3:43423986-43424005 None:intergenic 65.0%
GTCCGGACAGCCAGAACCAG+TGG + chr6.3:43424413-43424432 None:intergenic 65.0%
GTGGGGTTTCGCCGGAGAGT+TGG + chr6.3:43423988-43424007 None:intergenic 65.0%
TCCCCGCTGGGCAATCGATC+AGG + chr6.3:43424441-43424460 None:intergenic 65.0%
! AACCCTGCATCGATCCCCGC+TGG + chr6.3:43424454-43424473 None:intergenic 65.0%
! ACCCTGCATCGATCCCCGCT+GGG + chr6.3:43424453-43424472 None:intergenic 65.0%
! GGTGCCGGGGTGGTTGTAGA+GGG + chr6.3:43423846-43423865 None:intergenic 65.0%
! TGGTGCCGGGGTGGTTGTAG+AGG + chr6.3:43423847-43423866 None:intergenic 65.0%
GCCCAGCGGGGATCGATGCA+GGG - chr6.3:43424449-43424468 MS.gene30690:CDS 70.0%
GTCGCCATCGACGCCGACGT+CGG - chr6.3:43424103-43424122 MS.gene30690:CDS 70.0%
TGCCCAGCGGGGATCGATGC+AGG - chr6.3:43424448-43424467 MS.gene30690:CDS 70.0%
!! GATTGGTGGTGGTGCCGGGG+TGG + chr6.3:43423856-43423875 None:intergenic 70.0%
!! GTCGGCGTCGATGGCGACGA+CGG + chr6.3:43424101-43424120 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 43423836 43424882 43423836 ID=MS.gene30690
chr6.3 mRNA 43423836 43424882 43423836 ID=MS.gene30690.t1;Parent=MS.gene30690
chr6.3 exon 43423836 43424882 43423836 ID=MS.gene30690.t1.exon1;Parent=MS.gene30690.t1
chr6.3 CDS 43423836 43424882 43423836 ID=cds.MS.gene30690.t1;Parent=MS.gene30690.t1
Gene Sequence

>MS.gene30690

ATGCTATCCCTCTACAACCACCCCGGCACCACCACCAATCTCAAATCCCACTTCCCTTCAATCACCCATCTCCATCCCTCTCCCACATTCCCCAAACCCAAACCCAAAACCCTAACAACAAAATCCCTTCTTCAATGGAACCGCAAACCCCAACTCTCCGGCGAAACCCCACGCGTTGTAGTAGTCACATCCGGAAAAGGCGGAGTTGGTAAAACCACTACAACCGCTAACATCGGCCTTTCTTTAGCCCGTTTAGGCTTCTCCGTCGTCGCCATCGACGCCGACGTCGGATTACGTAACCTAGACCTTTTACTAGGTCTTGAAAATCGAGTCAACTACACCGTCGTTGAGATTCTCAACGGCGATTGTCGACTCGATCAAGCTTTGGTTAGAGATAAACGTTGGTCTAATTTTGAATTGCTTTGTATTTCTAAACCTAGATCTAAATTACCTATTGGATTTGGTGGAAAAGCTTTGACTTGGTTGGTTGAAGCTTTAAAAGCCCGGGGAGATAAAACCGGTGGAAATGGAAATGGAACTGGAACCAATGGTGGTTCTGGTTCCAACTCCAGTTCCACTGGTTCTGGCTGTCCGGACTTTATCCTGATCGATTGCCCAGCGGGGATCGATGCAGGGTTTATAACAGCCATTACGCCTGCTAATGAAGCGGTTTTGATAACAACGCCGGATATAACTGCATTAAGGGATGCAGATAGAGTGACGGGATTGTTGGAGTGTGATGGGATTAGGGATATTAAGATGATAGTGAATAGGGCTAGGACGGATATGATTAAAGGCGAAGATATGATGTCGGTTTTGGATGTGCAAGAGATGTTGGGTTTGCCGTTGCTTGGTGTTATACCGGAGGATAGTGAGGTTATAAGGAGTACGAATCGAGGGTATCCACTTGTTTTGAATAAGCCTCCTACTTTGGCTGGTTTGGCTTTTGAACAAGCTGCTTGGAGACTTGTGGAGCAGGATAGTATGCAAGCTGTTATGGTTGAGGAAGAGCCTAAAAGAGGGTTTTTCTCGTTTTTTGGTGGTTAA
Protein sequence

>MS.gene30690.t1

MLSLYNHPGTTTNLKSHFPSITHLHPSPTFPKPKPKTLTTKSLLQWNRKPQLSGETPRVVVVTSGKGGVGKTTTTANIGLSLARLGFSVVAIDADVGLRNLDLLLGLENRVNYTVVEILNGDCRLDQALVRDKRWSNFELLCISKPRSKLPIGFGGKALTWLVEALKARGDKTGGNGNGTGTNGGSGSNSSSTGSGCPDFILIDCPAGIDAGFITAITPANEAVLITTPDITALRDADRVTGLLECDGIRDIKMIVNRARTDMIKGEDMMSVLDVQEMLGLPLLGVIPEDSEVIRSTNRGYPLVLNKPPTLAGLAFEQAAWRLVEQDSMQAVMVEEEPKRGFFSFFGG