Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31107.t1 | XP_003597417.1 | 100 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 9.20E-41 | 175.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31107.t1 | Q9ZW33 | 65.8 | 79 | 27 | 0 | 1 | 79 | 1 | 79 | 1.4e-26 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31107.t1 | B7FH26 | 100.0 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 6.6e-41 | 175.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004041 | MS.gene31107 | PPI |
| MS.gene31107 | MS.gene071173 | PPI |
| MS.gene31107 | MS.gene41171 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31107.t1 | MTR_2g097870 | 100.000 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 1.10e-57 | 171 |
| MS.gene31107.t1 | MTR_2g097870 | 100.000 | 70 | 0 | 0 | 13 | 82 | 15 | 84 | 1.66e-48 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31107.t1 | AT2G29530 | 65.823 | 79 | 27 | 0 | 1 | 79 | 1 | 79 | 9.29e-38 | 121 |
| MS.gene31107.t1 | AT2G29530 | 65.823 | 79 | 27 | 0 | 1 | 79 | 1 | 79 | 9.29e-38 | 121 |
| MS.gene31107.t1 | AT2G29530 | 49.515 | 103 | 28 | 1 | 1 | 79 | 1 | 103 | 2.21e-31 | 105 |
Find 27 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTAATTTGAACAGATATT+TGG | 0.172661 | 2.4:+6104664 | MS.gene31107:intron |
| ACATCTTACATGCCAGTATT+TGG | 0.206917 | 2.4:-6105717 | None:intergenic |
| CAAGGAGTCCGAGCTAAATA+TGG | 0.278151 | 2.4:+6105664 | MS.gene31107:CDS |
| AATCTAATTGGTCAGCTTCT+TGG | 0.297313 | 2.4:+6106265 | MS.gene31107:CDS |
| ACCGCTGTGTTTCCAAATAC+TGG | 0.321334 | 2.4:+6105705 | MS.gene31107:CDS |
| TGTCAGGTGACTAATCTAAT+TGG | 0.348592 | 2.4:+6106253 | MS.gene31107:intron |
| ATTGGTCAGCTTCTTGGTAC+TGG | 0.406086 | 2.4:+6106271 | MS.gene31107:CDS |
| TGAAAAGGAGATGGAGTATA+GGG | 0.409805 | 2.4:+6104693 | MS.gene31107:CDS |
| CTGAAAAGGAGATGGAGTAT+AGG | 0.420828 | 2.4:+6104692 | MS.gene31107:CDS |
| GATATTTGGAATGGCTGAAA+AGG | 0.432718 | 2.4:+6104678 | MS.gene31107:intron |
| CACACTTATTGAAGCAGGAC+TGG | 0.454382 | 2.4:-6105536 | None:intergenic |
| TTCTGGTCTCGATAAAGATC+AGG | 0.485540 | 2.4:+6104441 | MS.gene31107:CDS |
| GTCAACACACTTATTGAAGC+AGG | 0.497289 | 2.4:-6105541 | None:intergenic |
| GTCAGCTTCTTGGTACTGGC+GGG | 0.500273 | 2.4:+6106275 | MS.gene31107:CDS |
| ACTTTATCTTCTCAGGTACA+AGG | 0.510118 | 2.4:+6105646 | MS.gene31107:intron |
| GGAAACACAGCGGTCAATGC+AGG | 0.526463 | 2.4:-6105696 | None:intergenic |
| ATTTGAACAGATATTTGGAA+TGG | 0.527257 | 2.4:+6104669 | MS.gene31107:intron |
| AAGGAGTCCGAGCTAAATAT+GGG | 0.527587 | 2.4:+6105665 | MS.gene31107:CDS |
| TGGAATGGCTGAAAAGGAGA+TGG | 0.540959 | 2.4:+6104684 | MS.gene31107:CDS |
| TGATCTTTATCGAGACCAGA+AGG | 0.553623 | 2.4:-6104439 | None:intergenic |
| GGTCAGCTTCTTGGTACTGG+CGG | 0.562832 | 2.4:+6106274 | MS.gene31107:CDS |
| ATCTTTATCGAGACCAGAAG+GGG | 0.578296 | 2.4:-6104437 | None:intergenic |
| GATCTTTATCGAGACCAGAA+GGG | 0.615418 | 2.4:-6104438 | None:intergenic |
| ACACTTATTGAAGCAGGACT+GGG | 0.620696 | 2.4:-6105535 | None:intergenic |
| GGTCTCGATAAAGATCAGGC+AGG | 0.638970 | 2.4:+6104445 | MS.gene31107:CDS |
| TCTTTATCGAGACCAGAAGG+GGG | 0.742894 | 2.4:-6104436 | None:intergenic |
| GCCAGTATTTGGAAACACAG+CGG | 0.771787 | 2.4:-6105706 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTTTTATTTTTATTTTC+TGG | + | chr2.4:6104577-6104596 | MS.gene31107:intron | 10.0% |
| !!! | AGTTTTTATTTTTATTTTCT+GGG | + | chr2.4:6104578-6104597 | MS.gene31107:intron | 10.0% |
| !! | AACTGATAAAAAAGTAAAAA+AGG | - | chr2.4:6104776-6104795 | None:intergenic | 15.0% |
| !! | AATTATATTTATGCAGTTTA+GGG | + | chr2.4:6105272-6105291 | MS.gene31107:intron | 15.0% |
| !! | GTTAAAAAAATATGCTAAAA+TGG | - | chr2.4:6106096-6106115 | None:intergenic | 15.0% |
| !! | TAATTATATTTATGCAGTTT+AGG | + | chr2.4:6105271-6105290 | MS.gene31107:intron | 15.0% |
| !! | TAAAAACTTGAAATCACAAA+CGG | - | chr2.4:6104566-6104585 | None:intergenic | 20.0% |
| !! | TGTTGGCATAATTATTAATT+TGG | + | chr2.4:6105479-6105498 | MS.gene31107:intron | 20.0% |
| !! | TTTCTTCAACTTAATTTCAT+AGG | - | chr2.4:6104995-6105014 | None:intergenic | 20.0% |
| !!! | GTTTGATTTTTTTTTCATTC+TGG | + | chr2.4:6104621-6104640 | MS.gene31107:intron | 20.0% |
| !!! | TGTTAATTTGAACAGATATT+TGG | + | chr2.4:6104664-6104683 | MS.gene31107:intron | 20.0% |
| !!! | TTTTTTGCAAATCCAATATT+TGG | + | chr2.4:6105792-6105811 | MS.gene31107:intron | 20.0% |
| ! | AAACAAAGTTGAGATCAAAA+TGG | - | chr2.4:6104521-6104540 | None:intergenic | 25.0% |
| ! | AACAAAGTTGAGATCAAAAT+GGG | - | chr2.4:6104520-6104539 | None:intergenic | 25.0% |
| ! | AAGAAGTTATGATCTGAATA+TGG | + | chr2.4:6105967-6105986 | MS.gene31107:intron | 25.0% |
| ! | AATAAGTTGTTTATCCAAAC+AGG | - | chr2.4:6104828-6104847 | None:intergenic | 25.0% |
| ! | AATTTCTGCAGCAAAAAAAT+TGG | - | chr2.4:6104493-6104512 | None:intergenic | 25.0% |
| ! | AGAAACAAACAACCAAATAT+TGG | - | chr2.4:6105807-6105826 | None:intergenic | 25.0% |
| ! | ATAGTTTCTCAAATGTCTAA+AGG | + | chr2.4:6105836-6105855 | MS.gene31107:intron | 25.0% |
| ! | ATTTGAACAGATATTTGGAA+TGG | + | chr2.4:6104669-6104688 | MS.gene31107:intron | 25.0% |
| ! | CTGAATGGTATAAACAAAAA+GGG | - | chr2.4:6105510-6105529 | None:intergenic | 25.0% |
| ! | GACAGATTTCATATACTTTA+GGG | + | chr2.4:6105246-6105265 | MS.gene31107:intron | 25.0% |
| ! | TAGTTTAGAGATTGAATTGA+TGG | + | chr2.4:6105316-6105335 | MS.gene31107:intron | 25.0% |
| ! | TGAATGGTATAAACAAAAAG+GGG | - | chr2.4:6105509-6105528 | None:intergenic | 25.0% |
| ! | TGACAGATTTCATATACTTT+AGG | + | chr2.4:6105245-6105264 | MS.gene31107:intron | 25.0% |
| ! | TTTGCAACTAGTTATAATGA+AGG | + | chr2.4:6105451-6105470 | MS.gene31107:intron | 25.0% |
| !! | TCTGAATATGGTTTTTACTT+TGG | + | chr2.4:6105979-6105998 | MS.gene31107:intron | 25.0% |
| !!! | GGATTTTCATATATTTTGAC+AGG | + | chr2.4:6105198-6105217 | MS.gene31107:intron | 25.0% |
| !!! | TCTAAAGGTACAATTTTAGT+TGG | + | chr2.4:6105851-6105870 | MS.gene31107:intron | 25.0% |
| !!! | TTATAATGAAGGTTTGTTGT+TGG | + | chr2.4:6105462-6105481 | MS.gene31107:intron | 25.0% |
| AAAACACCAGTTAGACAATA+AGG | - | chr2.4:6106226-6106245 | None:intergenic | 30.0% | |
| ACAAAGTTGAGATCAAAATG+GGG | - | chr2.4:6104519-6104538 | None:intergenic | 30.0% | |
| AGATGTTTCAAAAGCTATCT+TGG | + | chr2.4:6104965-6104984 | MS.gene31107:intron | 30.0% | |
| CATGATTCCAAAAGTAAAAG+AGG | - | chr2.4:6106174-6106193 | None:intergenic | 30.0% | |
| CCTGAATGGTATAAACAAAA+AGG | - | chr2.4:6105511-6105530 | None:intergenic | 30.0% | |
| TACACCATGGCTATTAAATA+CGG | + | chr2.4:6106064-6106083 | MS.gene31107:intron | 30.0% | |
| TATCACTCATAAGTCATAAC+AGG | - | chr2.4:6105613-6105632 | None:intergenic | 30.0% | |
| TGCAAAACAATTTAAGTGCT+TGG | + | chr2.4:6105940-6105959 | MS.gene31107:intron | 30.0% | |
| TGCAGCAAAAAAATTGGAAT+CGG | - | chr2.4:6104487-6104506 | None:intergenic | 30.0% | |
| TTAGGGACTAAGTTAGATAA+AGG | + | chr2.4:6105289-6105308 | MS.gene31107:intron | 30.0% | |
| TTCGAATAAACCATAGATGT+TGG | + | chr2.4:6104801-6104820 | MS.gene31107:intron | 30.0% | |
| ! | ATGTTCCTTATGTCTCTTTT+AGG | + | chr2.4:6105736-6105755 | MS.gene31107:intron | 30.0% |
| ! | TAGCAATCCTCTTTTACTTT+TGG | + | chr2.4:6106164-6106183 | MS.gene31107:intron | 30.0% |
| ! | TGAAACACAAGAACGATTTT+TGG | + | chr2.4:6106032-6106051 | MS.gene31107:intron | 30.0% |
| !! | ATAAGTTTTGTGAGGCTATT+TGG | - | chr2.4:6105057-6105076 | None:intergenic | 30.0% |
| !! | CCTTTTTGTTTATACCATTC+AGG | + | chr2.4:6105508-6105527 | MS.gene31107:intron | 30.0% |
| !! | GAAAATACTGATTTGTACGA+TGG | + | chr2.4:6106001-6106020 | MS.gene31107:intron | 30.0% |
| !! | TAAGTTTTGTGAGGCTATTT+GGG | - | chr2.4:6105056-6105075 | None:intergenic | 30.0% |
| !!! | CTCTTTTACTTTTGGAATCA+TGG | + | chr2.4:6106172-6106191 | MS.gene31107:intron | 30.0% |
| !!! | GCTTACATAAGCTACTTTTA+TGG | + | chr2.4:6104900-6104919 | MS.gene31107:intron | 30.0% |
| !!! | TAAGCTACTTTTATGGCAAA+TGG | + | chr2.4:6104907-6104926 | MS.gene31107:intron | 30.0% |
| AATCTAATTGGTCAGCTTCT+TGG | + | chr2.4:6106265-6106284 | MS.gene31107:CDS | 35.0% | |
| ACATCTTACATGCCAGTATT+TGG | - | chr2.4:6105720-6105739 | None:intergenic | 35.0% | |
| ACGAAGTTACTGAACACAAA+AGG | - | chr2.4:6106138-6106157 | None:intergenic | 35.0% | |
| ACTTTATCTTCTCAGGTACA+AGG | + | chr2.4:6105646-6105665 | MS.gene31107:intron | 35.0% | |
| AGACAATAAGGCAGTGATTA+TGG | - | chr2.4:6106214-6106233 | None:intergenic | 35.0% | |
| AGAGTTAGAGATCAAACTGA+TGG | + | chr2.4:6105177-6105196 | MS.gene31107:intron | 35.0% | |
| ATGTCCGTATTTAATAGCCA+TGG | - | chr2.4:6106071-6106090 | None:intergenic | 35.0% | |
| CCAATCCTAAAAGAGACATA+AGG | - | chr2.4:6105744-6105763 | None:intergenic | 35.0% | |
| GAACTATAGTTTAGAGACCA+GGG | - | chr2.4:6105117-6105136 | None:intergenic | 35.0% | |
| GGCTTATTTGAGCTTATCTA+CGG | - | chr2.4:6105082-6105101 | None:intergenic | 35.0% | |
| GGTTGAATTGTTCAACAAGT+AGG | + | chr2.4:6104714-6104733 | MS.gene31107:intron | 35.0% | |
| TGAAAAGGAGATGGAGTATA+GGG | + | chr2.4:6104693-6104712 | MS.gene31107:CDS | 35.0% | |
| TGTCAGGTGACTAATCTAAT+TGG | + | chr2.4:6106253-6106272 | MS.gene31107:intron | 35.0% | |
| ! | GATATTTGGAATGGCTGAAA+AGG | + | chr2.4:6104678-6104697 | MS.gene31107:intron | 35.0% |
| !! | GTTTGTCACTTTATCTTCTC+AGG | + | chr2.4:6105639-6105658 | MS.gene31107:intron | 35.0% |
| !!! | ATGTCTCTTTTAGGATTGGA+TGG | + | chr2.4:6105745-6105764 | MS.gene31107:intron | 35.0% |
| !!! | CCTTATGTCTCTTTTAGGAT+TGG | + | chr2.4:6105741-6105760 | MS.gene31107:intron | 35.0% |
| !!! | TGGTGTTTTTTGTTGTTGTC+AGG | + | chr2.4:6106237-6106256 | MS.gene31107:intron | 35.0% |
| !!! | TGTCTCTTTTAGGATTGGAT+GGG | + | chr2.4:6105746-6105765 | MS.gene31107:intron | 35.0% |
| AAGGAGTCCGAGCTAAATAT+GGG | + | chr2.4:6105665-6105684 | MS.gene31107:CDS | 40.0% | |
| ACACTTATTGAAGCAGGACT+GGG | - | chr2.4:6105538-6105557 | None:intergenic | 40.0% | |
| ATCTTTATCGAGACCAGAAG+GGG | - | chr2.4:6104440-6104459 | None:intergenic | 40.0% | |
| CGAACTATAGTTTAGAGACC+AGG | - | chr2.4:6105118-6105137 | None:intergenic | 40.0% | |
| CTGAAAAGGAGATGGAGTAT+AGG | + | chr2.4:6104692-6104711 | MS.gene31107:CDS | 40.0% | |
| CTGTCATGTTCTCTTGCTTT+CGG | + | chr2.4:6105384-6105403 | MS.gene31107:intron | 40.0% | |
| GATCTTTATCGAGACCAGAA+GGG | - | chr2.4:6104441-6104460 | None:intergenic | 40.0% | |
| GTCAACACACTTATTGAAGC+AGG | - | chr2.4:6105544-6105563 | None:intergenic | 40.0% | |
| TGATCTTTATCGAGACCAGA+AGG | - | chr2.4:6104442-6104461 | None:intergenic | 40.0% | |
| TTCTGGTCTCGATAAAGATC+AGG | + | chr2.4:6104441-6104460 | MS.gene31107:CDS | 40.0% | |
| ! | GTTTTCGCCCATATTTAGCT+CGG | - | chr2.4:6105675-6105694 | None:intergenic | 40.0% |
| ! | TCACTGCCTTATTGTCTAAC+TGG | + | chr2.4:6106217-6106236 | MS.gene31107:intron | 40.0% |
| !! | CTACGGTCATAAGTTTTGTG+AGG | - | chr2.4:6105065-6105084 | None:intergenic | 40.0% |
| !! | TTGGCTGTTGATTTACACCA+TGG | + | chr2.4:6106051-6106070 | MS.gene31107:intron | 40.0% |
| ACCGCTGTGTTTCCAAATAC+TGG | + | chr2.4:6105705-6105724 | MS.gene31107:CDS | 45.0% | |
| ATTGGTCAGCTTCTTGGTAC+TGG | + | chr2.4:6106271-6106290 | MS.gene31107:CDS | 45.0% | |
| CAAGGAGTCCGAGCTAAATA+TGG | + | chr2.4:6105664-6105683 | MS.gene31107:CDS | 45.0% | |
| CACACTTATTGAAGCAGGAC+TGG | - | chr2.4:6105539-6105558 | None:intergenic | 45.0% | |
| CCAAACAGGTCCAACATCTA+TGG | - | chr2.4:6104814-6104833 | None:intergenic | 45.0% | |
| CCATAGATGTTGGACCTGTT+TGG | + | chr2.4:6104811-6104830 | MS.gene31107:intron | 45.0% | |
| GCCAGTATTTGGAAACACAG+CGG | - | chr2.4:6105709-6105728 | None:intergenic | 45.0% | |
| TCTTTATCGAGACCAGAAGG+GGG | - | chr2.4:6104439-6104458 | None:intergenic | 45.0% | |
| TGGAATGGCTGAAAAGGAGA+TGG | + | chr2.4:6104684-6104703 | MS.gene31107:CDS | 45.0% | |
| ! | TTTAGAGACCAGGGCTTGTT+TGG | - | chr2.4:6105108-6105127 | None:intergenic | 45.0% |
| AAGCCAATCCAAACAAGCCC+TGG | + | chr2.4:6105097-6105116 | MS.gene31107:intron | 50.0% | |
| GGTCTCGATAAAGATCAGGC+AGG | + | chr2.4:6104445-6104464 | MS.gene31107:CDS | 50.0% | |
| ! | AGACCAGGGCTTGTTTGGAT+TGG | - | chr2.4:6105103-6105122 | None:intergenic | 50.0% |
| CAGGACTGGGTCATCCTGAA+TGG | - | chr2.4:6105525-6105544 | None:intergenic | 55.0% | |
| GGAAACACAGCGGTCAATGC+AGG | - | chr2.4:6105699-6105718 | None:intergenic | 55.0% | |
| ! | GGTCAGCTTCTTGGTACTGG+CGG | + | chr2.4:6106274-6106293 | MS.gene31107:CDS | 55.0% |
| !! | GTCAGCTTCTTGGTACTGGC+GGG | + | chr2.4:6106275-6106294 | MS.gene31107:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 6104428 | 6106306 | 6104428 | ID=MS.gene31107 |
| chr2.4 | mRNA | 6104428 | 6106306 | 6104428 | ID=MS.gene31107.t1;Parent=MS.gene31107 |
| chr2.4 | exon | 6104428 | 6104466 | 6104428 | ID=MS.gene31107.t1.exon1;Parent=MS.gene31107.t1 |
| chr2.4 | CDS | 6104428 | 6104466 | 6104428 | ID=cds.MS.gene31107.t1;Parent=MS.gene31107.t1 |
| chr2.4 | exon | 6104679 | 6104731 | 6104679 | ID=MS.gene31107.t1.exon2;Parent=MS.gene31107.t1 |
| chr2.4 | CDS | 6104679 | 6104731 | 6104679 | ID=cds.MS.gene31107.t1;Parent=MS.gene31107.t1 |
| chr2.4 | exon | 6105530 | 6105568 | 6105530 | ID=MS.gene31107.t1.exon3;Parent=MS.gene31107.t1 |
| chr2.4 | CDS | 6105530 | 6105568 | 6105530 | ID=cds.MS.gene31107.t1;Parent=MS.gene31107.t1 |
| chr2.4 | exon | 6105661 | 6105730 | 6105661 | ID=MS.gene31107.t1.exon4;Parent=MS.gene31107.t1 |
| chr2.4 | CDS | 6105661 | 6105730 | 6105661 | ID=cds.MS.gene31107.t1;Parent=MS.gene31107.t1 |
| chr2.4 | exon | 6106259 | 6106306 | 6106259 | ID=MS.gene31107.t1.exon5;Parent=MS.gene31107.t1 |
| chr2.4 | CDS | 6106259 | 6106306 | 6106259 | ID=cds.MS.gene31107.t1;Parent=MS.gene31107.t1 |
| Gene Sequence |
| Protein sequence |
