AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31129

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31129.t1 MTR_2g097467 90.244 656 35 2 1 627 1 656 0.0 1191
MS.gene31129.t1 MTR_2g097473 87.652 656 52 2 1 627 1 656 0.0 1166
MS.gene31129.t1 MTR_2g097463 79.452 657 105 2 1 627 1 657 0.0 1056
MS.gene31129.t1 MTR_4g064200 54.873 667 246 13 1 627 1 652 0.0 685
MS.gene31129.t1 MTR_7g062120 42.628 685 285 12 43 627 66 742 4.04e-178 523
MS.gene31129.t1 MTR_4g064160 53.778 450 189 6 190 627 244 686 6.57e-167 493
MS.gene31129.t1 MTR_4g064120 44.795 634 282 16 37 627 20 628 3.04e-165 486
MS.gene31129.t1 MTR_4g064150 42.708 672 289 18 37 627 79 735 1.76e-163 486
MS.gene31129.t1 MTR_4g064180 44.637 634 283 16 37 627 20 628 3.53e-162 479
MS.gene31129.t1 MTR_2g097410 51.461 445 210 3 189 627 299 743 7.96e-162 481
MS.gene31129.t1 MTR_2g097310 38.040 602 335 12 50 627 53 640 1.37e-138 418
MS.gene31129.t1 MTR_2g097390 37.953 635 348 13 22 627 26 643 1.84e-138 418
MS.gene31129.t1 MTR_2g097350 45.622 434 221 7 200 627 218 642 6.73e-132 401
MS.gene31129.t1 MTR_2g097380 48.361 366 159 5 263 627 227 563 2.78e-121 371
MS.gene31129.t1 MTR_5g097480 32.283 381 252 5 248 624 165 543 1.06e-58 206
MS.gene31129.t1 MTR_2g082090 32.225 391 259 5 238 624 190 578 3.11e-57 203
MS.gene31129.t1 MTR_2g026250 29.178 377 262 4 252 624 220 595 1.69e-56 201
MS.gene31129.t1 MTR_4g133660 29.843 382 261 6 249 625 164 543 1.42e-54 195
MS.gene31129.t1 MTR_7g027190 30.102 392 242 5 253 624 293 672 3.92e-53 193
MS.gene31129.t1 MTR_1g029420 29.076 368 257 3 260 624 225 591 3.75e-52 189
MS.gene31129.t1 MTR_4g104020 29.870 385 242 7 257 624 146 519 8.26e-52 187
MS.gene31129.t1 MTR_3g056110 28.928 401 278 4 230 624 142 541 9.37e-52 187
MS.gene31129.t1 MTR_5g094450 30.952 378 249 8 256 624 157 531 4.02e-51 185
MS.gene31129.t1 MTR_3g089055 28.272 382 268 4 248 624 189 569 8.25e-50 182
MS.gene31129.t1 MTR_3g089055 28.272 382 268 4 248 624 184 564 8.31e-50 182
MS.gene31129.t1 MTR_8g442410 28.906 384 252 5 254 624 149 524 9.08e-49 179
MS.gene31129.t1 MTR_3g065980 29.897 388 241 8 254 624 166 539 6.08e-46 171
MS.gene31129.t1 MTR_7g074650 29.700 367 241 7 263 626 441 793 8.53e-44 168
MS.gene31129.t1 MTR_3g022830 27.338 417 243 7 261 624 26 435 1.42e-41 156
MS.gene31129.t1 MTR_2g034260 29.716 387 247 8 255 624 205 583 6.91e-40 154
MS.gene31129.t1 MTR_4g076140 25.814 430 251 5 257 624 45 468 1.06e-38 149
MS.gene31129.t1 MTR_2g034250 28.462 390 249 8 255 624 205 584 1.17e-37 148
MS.gene31129.t1 MTR_4g102790 25.130 386 264 9 256 626 288 663 2.03e-37 148
MS.gene31129.t1 MTR_4g076020 28.382 377 226 11 263 627 87 431 1.19e-36 142
MS.gene31129.t1 MTR_2g089100 25.686 401 258 8 260 627 52 445 6.08e-35 138
MS.gene31129.t1 MTR_4g077760 25.995 377 258 7 257 624 190 554 7.51e-34 136
MS.gene31129.t1 MTR_4g097080 24.934 377 268 7 260 626 133 504 8.67e-34 135
MS.gene31129.t1 MTR_7g109580 26.683 416 263 10 224 624 165 553 6.94e-33 133
MS.gene31129.t1 MTR_1g069725 26.509 381 249 12 254 624 103 462 1.76e-32 131
MS.gene31129.t1 MTR_2g034280 26.893 383 261 8 255 624 198 574 4.89e-32 131
MS.gene31129.t1 MTR_1g096030 26.225 408 256 12 248 624 65 458 7.15e-32 129
MS.gene31129.t1 MTR_5g015490 25.260 384 264 8 260 626 114 491 1.75e-31 128
MS.gene31129.t1 MTR_5g058860 27.424 361 228 12 287 625 149 497 5.62e-29 121
MS.gene31129.t1 MTR_3g072710 28.418 373 222 14 279 624 145 499 1.83e-28 120
MS.gene31129.t1 MTR_7g057230 27.046 281 195 5 346 624 384 656 5.55e-26 113
MS.gene31129.t1 MTR_6g047750 26.437 348 227 10 292 624 328 661 2.77e-25 111
MS.gene31129.t1 MTR_1g086970 24.011 354 242 8 287 624 135 477 3.00e-24 107
MS.gene31129.t1 MTR_5g009080 31.100 209 134 2 260 464 56 258 3.62e-24 106
MS.gene31129.t1 MTR_5g009080 29.530 149 105 0 476 624 329 477 5.61e-17 84.3
MS.gene31129.t1 MTR_5g015950 21.823 417 274 9 260 627 35 448 1.29e-23 104
MS.gene31129.t1 MTR_3g053270 21.823 417 274 9 260 627 35 448 1.29e-23 104
MS.gene31129.t1 MTR_8g093070 23.747 379 249 16 265 627 144 498 1.13e-22 102
MS.gene31129.t1 MTR_2g099110 25.500 400 244 11 260 626 74 452 2.74e-22 100
MS.gene31129.t1 MTR_4g095500 23.206 418 265 10 260 627 59 470 3.53e-22 100
MS.gene31129.t1 MTR_4g026485 22.194 392 273 10 246 624 251 623 3.80e-17 85.5
MS.gene31129.t1 MTR_0092s0100 23.181 371 262 9 261 624 373 727 1.49e-16 84.0
MS.gene31129.t1 MTR_0092s0100 23.181 371 262 9 261 624 376 730 1.53e-16 84.0
MS.gene31129.t1 MTR_8g020840 25.250 400 241 15 260 624 171 547 2.82e-16 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31129.t1 AT2G29060 44.079 608 303 14 49 626 56 656 1.37e-167 493
MS.gene31129.t1 AT5G59450 44.822 589 262 12 53 627 61 600 1.05e-165 487
MS.gene31129.t1 AT2G29060 42.326 645 298 16 49 626 56 693 1.14e-162 482
MS.gene31129.t1 AT2G37650 42.065 649 294 13 49 626 65 702 1.26e-160 477
MS.gene31129.t1 AT2G37650 42.065 649 294 13 49 626 77 714 2.30e-160 477
MS.gene31129.t1 AT1G07530 49.675 461 220 4 179 627 307 767 2.63e-156 468
MS.gene31129.t1 AT3G46600 51.562 448 194 6 188 627 117 549 3.09e-153 453
MS.gene31129.t1 AT3G46600 51.562 448 194 6 188 627 149 581 3.92e-153 454
MS.gene31129.t1 AT3G46600 55.946 370 161 1 260 627 4 373 1.89e-150 439
MS.gene31129.t1 AT2G29065 42.434 608 309 13 50 627 34 630 3.96e-147 440
MS.gene31129.t1 AT1G07520 46.429 448 222 6 190 627 92 531 6.54e-136 408
MS.gene31129.t1 AT1G07520 46.429 448 222 6 190 627 92 531 6.54e-136 408
MS.gene31129.t1 AT1G07520 46.429 448 222 6 190 627 256 695 2.89e-134 409
MS.gene31129.t1 AT1G07520 46.429 448 222 6 190 627 257 696 3.14e-134 409
MS.gene31129.t1 AT1G50600 33.002 403 262 6 227 624 126 525 5.44e-60 209
MS.gene31129.t1 AT1G50600 33.002 403 262 6 227 624 197 596 2.56e-59 209
MS.gene31129.t1 AT1G50600 33.002 403 262 6 227 624 201 600 4.52e-59 208
MS.gene31129.t1 AT5G48150 31.414 382 257 3 247 624 109 489 1.63e-58 204
MS.gene31129.t1 AT5G48150 31.414 382 257 3 247 624 109 489 1.63e-58 204
MS.gene31129.t1 AT5G48150 31.414 382 257 3 247 624 109 489 1.63e-58 204
MS.gene31129.t1 AT5G48150 31.414 382 257 3 247 624 109 489 1.63e-58 204
MS.gene31129.t1 AT2G04890 30.376 372 248 4 256 624 49 412 4.05e-52 185
MS.gene31129.t1 AT4G17230 30.667 375 256 3 255 626 153 526 3.43e-49 180
MS.gene31129.t1 AT4G17230 30.667 375 256 3 255 626 153 526 3.43e-49 180
MS.gene31129.t1 AT1G21450 27.989 368 262 2 260 624 225 592 2.02e-47 176
MS.gene31129.t1 AT1G14920 29.929 421 261 11 226 624 120 528 2.10e-47 175
MS.gene31129.t1 AT3G03450 30.100 402 235 10 248 624 164 544 7.25e-47 174
MS.gene31129.t1 AT1G66350 30.612 392 237 11 247 624 135 505 1.37e-45 169
MS.gene31129.t1 AT3G54220 30.563 373 233 9 263 626 296 651 4.93e-45 170
MS.gene31129.t1 AT5G17490 30.829 386 228 11 254 624 154 515 7.67e-44 165
MS.gene31129.t1 AT2G01570 28.866 388 253 10 248 624 205 580 1.78e-43 165
MS.gene31129.t1 AT5G66770 28.954 373 254 8 257 624 217 583 1.25e-42 162
MS.gene31129.t1 AT5G41920 27.344 384 251 7 251 624 34 399 2.07e-39 150
MS.gene31129.t1 AT3G50650 26.133 375 260 7 261 624 173 541 5.56e-37 145
MS.gene31129.t1 AT4G37650 25.558 403 248 11 259 626 145 530 2.94e-35 140
MS.gene31129.t1 AT1G63100 25.575 391 261 7 256 625 273 654 1.34e-32 134
MS.gene31129.t1 AT1G63100 25.575 391 261 7 256 625 248 629 1.36e-32 133
MS.gene31129.t1 AT5G52510 27.105 380 248 10 263 624 271 639 1.49e-32 133
MS.gene31129.t1 AT3G49950 26.562 384 261 9 260 626 30 409 1.21e-30 125
MS.gene31129.t1 AT4G08250 28.846 364 232 13 276 624 125 476 1.26e-29 123
MS.gene31129.t1 AT1G50420 28.761 226 146 4 257 473 54 273 2.44e-23 104
MS.gene31129.t1 AT1G50420 29.530 149 105 0 476 624 330 478 1.81e-17 86.3
MS.gene31129.t1 AT1G55580 22.739 387 233 8 289 624 73 444 1.37e-22 101
MS.gene31129.t1 AT3G13840 23.773 387 248 14 260 624 148 509 3.60e-19 91.7
MS.gene31129.t1 AT4G00150 22.196 428 292 11 204 624 160 553 3.87e-14 75.9
MS.gene31129.t1 AT5G67411 29.703 202 125 9 373 561 1 198 1.55e-11 64.3

Find 133 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTCAGTGCTACCATTT+GGG 0.231237 2.4:+6328346 None:intergenic
TATGTATCTTAGGATGTTAT+TGG 0.255000 2.4:+6328491 None:intergenic
AGCGTTCATGTTATTGATTT+TGG 0.271020 2.4:-6327475 MS.gene31129:CDS
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2.4:-6327391 MS.gene31129:CDS
CACTATTTCGAAAATCGTTT+TGG 0.292516 2.4:+6327510 None:intergenic
TCATCATCTTCACCTTCTTC+TGG 0.302876 2.4:-6328555 MS.gene31129:CDS
AGGCAACACTCTTCGCCTTT+TGG 0.305061 2.4:-6327706 MS.gene31129:CDS
ACTCCATGGAAGAAGACTTT+TGG 0.314241 2.4:+6327097 None:intergenic
GTTACTTGGTTTACCGAGTT+TGG 0.314473 2.4:+6328600 None:intergenic
TTCCTTCTTCACCATTGCTT+TGG 0.317965 2.4:+6326793 None:intergenic
TCTTGGTCAGTGCTACCATT+TGG 0.323016 2.4:+6328345 None:intergenic
GCAGGAATGTTTAAGACTTC+AGG 0.330542 2.4:-6328415 MS.gene31129:CDS
TTTGTATGCTTTATCTGCTT+GGG 0.337273 2.4:-6326705 MS.gene31129:CDS
ATAACTCATATGAATCTAAC+TGG 0.344405 2.4:-6328266 MS.gene31129:CDS
AAGTCCTTCTATGATGTTCT+TGG 0.346747 2.4:-6328384 MS.gene31129:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.355828 2.4:-6327667 MS.gene31129:CDS
CAAAATTATAAGATGCTTGT+TGG 0.356875 2.4:+6327887 None:intergenic
GGATGTCACTTATGTATCTT+AGG 0.358360 2.4:+6328481 None:intergenic
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2.4:+6327388 None:intergenic
AATCAGGTTGGATTCTGATT+TGG 0.361148 2.4:-6326825 MS.gene31129:CDS
TAAAGGAGTTTGTAGTTGTA+TGG 0.363980 2.4:+6328212 None:intergenic
ACTCAAACCCATTTGTTACT+TGG 0.366037 2.4:+6328586 None:intergenic
GCAGAAAGGGTGGAGGAAAC+TGG 0.374425 2.4:-6327337 MS.gene31129:CDS
CATCGACTACAAAATCATTA+TGG 0.375138 2.4:+6326768 None:intergenic
AAATCTTGCAGAAAGGTGTT+TGG 0.378133 2.4:+6328630 None:intergenic
CCTCTTGGTTTGGCAACTGC+TGG 0.385884 2.4:-6327604 MS.gene31129:CDS
ATCGCTGTTTGATATGTTTG+AGG 0.388834 2.4:-6327008 MS.gene31129:CDS
AGCTTCAACACAGCTTCTCT+TGG 0.390505 2.4:+6327136 None:intergenic
ATCATATCAGCCTCTTGGTT+TGG 0.393034 2.4:-6327614 MS.gene31129:CDS
CCCCGTGTCTCGATGACAAC+TGG 0.393627 2.4:+6328153 None:intergenic
GTTTCCTCCACCCTTTCTGC+AGG 0.394328 2.4:+6327340 None:intergenic
AAGCTGTGTTGAAGCTGATC+AGG 0.396558 2.4:-6327129 MS.gene31129:CDS
TCTTCTGGCACAAGCTCAAA+TGG 0.409923 2.4:-6328540 MS.gene31129:CDS
ACCATTGTCACAACGGAACT+TGG 0.411323 2.4:-6328125 MS.gene31129:CDS
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2.4:-6326841 MS.gene31129:CDS
TCGGTAAACCAAGTAACAAA+TGG 0.413464 2.4:-6328594 MS.gene31129:CDS
CACGATCGAAGTTTCCATCT+TGG 0.422939 2.4:+6328328 None:intergenic
TTCTGACGAGGCACGGCCAC+TGG 0.426845 2.4:+6327437 None:intergenic
CGTACAAGCAGTGGCAGGTC+AGG 0.432935 2.4:-6326871 MS.gene31129:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.436357 2.4:-6326953 MS.gene31129:CDS
TTCAATGCCACAGTTTCATC+AGG 0.440053 2.4:+6327163 None:intergenic
AAGCTCAAATGGAGAATCCT+TGG 0.456686 2.4:-6328529 MS.gene31129:CDS
TGCCACTGCTTGTACGTCTC+AGG 0.458186 2.4:+6326878 None:intergenic
CATTTGGGGATGGATGGTTA+TGG 0.459747 2.4:+6328361 None:intergenic
CATGGATGAAGAAGATGACT+TGG 0.465816 2.4:-6328457 MS.gene31129:CDS
TTGAGAGAGGGTTGTTTGGA+AGG 0.472118 2.4:-6326949 MS.gene31129:CDS
GAAACTTCTTCCACCGTCAC+TGG 0.475504 2.4:+6328084 None:intergenic
ATGTTGCGGCTGTTGATTTG+TGG 0.475907 2.4:-6327804 MS.gene31129:CDS
TGCTACCATTTGGGGATGGA+TGG 0.476955 2.4:+6328355 None:intergenic
GTGCTTCCTTAAACCGAGTC+AGG 0.477530 2.4:+6327044 None:intergenic
TTTGAGCTTGTGCCAGAAGA+AGG 0.477829 2.4:+6328543 None:intergenic
AGCACCTCATCTAAAATCTC+TGG 0.485983 2.4:+6327943 None:intergenic
AGCTTGTGAGGGAGCAGAAA+GGG 0.488617 2.4:-6326909 MS.gene31129:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.490039 2.4:+6327352 None:intergenic
CGGTAAACCAAGTAACAAAT+GGG 0.492040 2.4:-6328593 MS.gene31129:CDS
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2.4:-6326754 MS.gene31129:CDS
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2.4:+6327400 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2.4:+6327391 None:intergenic
AACCAAAGCAATGGTGAAGA+AGG 0.500696 2.4:-6326795 MS.gene31129:CDS
ATGGCCAAGAACATCATAGA+AGG 0.501527 2.4:+6328380 None:intergenic
AATCCAAAAGTCTTCTTCCA+TGG 0.502095 2.4:-6327100 MS.gene31129:CDS
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2.4:-6327650 MS.gene31129:CDS
GGAATTGAGCTTCCTCAGCC+AGG 0.503697 2.4:-6327370 MS.gene31129:CDS
CAGGTCAGGAACAAGAGAGC+TGG 0.506156 2.4:-6326857 MS.gene31129:CDS
CTTGAGATCCTTGAGACGGA+TGG 0.508334 2.4:+6327240 None:intergenic
TTCTTCCATGGAGTTGCCAA+TGG 0.510258 2.4:-6327088 MS.gene31129:CDS
ATGAGCTACTCAAACAGATT+AGG 0.511922 2.4:-6327726 MS.gene31129:CDS
AGGATTTAGACCTGCAGAAA+GGG 0.512549 2.4:-6327350 MS.gene31129:CDS
GGAATATCTTGTCATTTCCA+AGG 0.512718 2.4:+6328512 None:intergenic
TCAAGGATCTCAAGATAGAC+AGG 0.512857 2.4:-6327231 MS.gene31129:CDS
AAACCAAGAGGCTGATATGA+TGG 0.515320 2.4:+6327616 None:intergenic
CAACTTCATTGCACAGAGAT+GGG 0.518376 2.4:-6327266 MS.gene31129:CDS
ACGCCATCATATCAGCCTCT+TGG 0.518385 2.4:-6327619 MS.gene31129:CDS
ACAACTTCATTGCACAGAGA+TGG 0.523539 2.4:-6327267 MS.gene31129:CDS
CGTTGGAGTCCATTGCCAAA+AGG 0.523700 2.4:+6327691 None:intergenic
TCCAGTTGTCATCGAGACAC+GGG 0.534599 2.4:-6328154 MS.gene31129:CDS
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2.4:-6327756 MS.gene31129:CDS
TCCAAGTTCCGTTGTGACAA+TGG 0.537164 2.4:+6328124 None:intergenic
TGAGAGAGGGTTGTTTGGAA+GGG 0.539295 2.4:-6326948 MS.gene31129:CDS
AACTTCCATTGGCAACTCCA+TGG 0.540581 2.4:+6327083 None:intergenic
CATAACCATCCATCCCCAAA+TGG 0.544430 2.4:-6328360 MS.gene31129:CDS
GTCACAACGGAACTTGGAGT+TGG 0.544454 2.4:-6328119 MS.gene31129:CDS
TATAAATGTTATAGCTTGTG+AGG 0.544729 2.4:-6326921 MS.gene31129:CDS
CAGGATTTAGACCTGCAGAA+AGG 0.549603 2.4:-6327351 MS.gene31129:CDS
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2.4:+6327277 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2.4:-6326753 MS.gene31129:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2.4:+6327072 None:intergenic
AACACTAAAATCTTGCAGAA+AGG 0.557457 2.4:+6328623 None:intergenic
TTGTGCACATTGATTTAGCA+TGG 0.558170 2.4:+6327780 None:intergenic
GCATTTGTCATTCTCTGCAA+AGG 0.559014 2.4:+6327541 None:intergenic
AAACATTCCTGCAACATGCA+TGG 0.559801 2.4:+6328426 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2.4:+6327167 None:intergenic
ATCCAGTTGTCATCGAGACA+CGG 0.568814 2.4:-6328155 MS.gene31129:CDS
AATGGTAGCACTGACCAAGA+TGG 0.569750 2.4:-6328342 MS.gene31129:CDS
CAAAGTAATGAGCTAAACGT+TGG 0.573182 2.4:+6327674 None:intergenic
TTGGTCAGTGCTACCATTTG+GGG 0.574197 2.4:+6328347 None:intergenic
TGTAATGAATAACAGGGAAG+AGG 0.575635 2.4:-6328006 MS.gene31129:intron
AGGTCAGGAACAAGAGAGCT+GGG 0.576170 2.4:-6326856 MS.gene31129:CDS
ATACATAAGTGACATCCTCA+TGG 0.582153 2.4:-6328475 MS.gene31129:CDS
ATGGGAAACCATCCGTCTCA+AGG 0.585121 2.4:-6327248 MS.gene31129:CDS
AGGGAGCAGAAAGGGTTGAA+AGG 0.587349 2.4:-6326901 MS.gene31129:CDS
GAGGTGCTGCTTTATCAGAC+AGG 0.590907 2.4:-6327928 MS.gene31129:CDS
TGAGACGTACAAGCAGTGGC+AGG 0.594436 2.4:-6326876 MS.gene31129:CDS
CAGAGGTCGATGCTTGAGAG+AGG 0.600053 2.4:-6326962 MS.gene31129:CDS
TGATTCAGGAGGAAAGGCAA+AGG 0.600358 2.4:-6327866 MS.gene31129:CDS
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2.4:-6327347 MS.gene31129:CDS
TCAGTGCTACCATTTGGGGA+TGG 0.608274 2.4:+6328351 None:intergenic
ACCTCATCTAAAATCTCTGG+TGG 0.612420 2.4:+6327946 None:intergenic
ATAAATGTTATAGCTTGTGA+GGG 0.613447 2.4:-6326920 MS.gene31129:CDS
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2.4:-6326837 MS.gene31129:CDS
GCAGGTCTAAATCCTGGCTG+AGG 0.615245 2.4:+6327358 None:intergenic
GGCCTGAGACGTACAAGCAG+TGG 0.615272 2.4:-6326880 MS.gene31129:CDS
AAGAGTCGAATTCGACGACA+TGG 0.616744 2.4:+6328238 None:intergenic
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2.4:-6327344 MS.gene31129:CDS
TAGCTTGTGAGGGAGCAGAA+AGG 0.625836 2.4:-6326910 MS.gene31129:CDS
CTATCTTGAGATCCTTGAGA+CGG 0.633125 2.4:+6327236 None:intergenic
CCAGCAGTTGCCAAACCAAG+AGG 0.642152 2.4:+6327604 None:intergenic
GACCTCTGCGTGTCTTCACG+AGG 0.643181 2.4:+6326977 None:intergenic
ATAACAGGGAAGAGGTAGCA+AGG 0.645504 2.4:-6327998 MS.gene31129:intron
AGAAAACTTTCATTAAGACC+TGG 0.645902 2.4:-6327418 MS.gene31129:CDS
GAATGACATTCAGCCAGTGA+CGG 0.650343 2.4:-6328097 MS.gene31129:intron
AGAGGTCGATGCTTGAGAGA+GGG 0.653291 2.4:-6326961 MS.gene31129:CDS
GGTGAATGAAACCAAAGCAA+TGG 0.658724 2.4:-6326804 MS.gene31129:CDS
GAACCTTCCTGATGAAACTG+TGG 0.660816 2.4:-6327170 MS.gene31129:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2.4:-6327773 MS.gene31129:CDS
AAAGGGTGGAGGAAACTGGA+CGG 0.662270 2.4:-6327333 MS.gene31129:CDS
GGTGCTAACCATTGTCACAA+CGG 0.663777 2.4:-6328132 MS.gene31129:CDS
AAACTTTCATTAAGACCTGG+TGG 0.664266 2.4:-6327415 MS.gene31129:CDS
CCAGTTGTCATCGAGACACG+GGG 0.664986 2.4:-6328153 MS.gene31129:CDS
AGATTCATATGAGTTATCAG+TGG 0.666994 2.4:+6328272 None:intergenic
TAACTCATATGAATCTAACT+GGG 0.675488 2.4:-6328265 MS.gene31129:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2.4:+6328460 None:intergenic
TGCCTCGTGAAGACACGCAG+AGG 0.691967 2.4:-6326979 MS.gene31129:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTAGTTTTTTTCTATTAA+AGG + chr2.4:6327141-6327160 None:intergenic 15.0%
!!! ATTGATTTTGGAATTTTCTA+TGG - chr2.4:6327870-6327889 MS.gene31129:CDS 20.0%
! AGAAAAAAGAAGTCATAAGA+TGG - chr2.4:6327287-6327306 MS.gene31129:CDS 25.0%
! ATAACTCATATGAATCTAAC+TGG - chr2.4:6327067-6327086 MS.gene31129:CDS 25.0%
! CAAAATTATAAGATGCTTGT+TGG + chr2.4:6327449-6327468 None:intergenic 25.0%
! GATACTTGTAATGAATAACA+GGG - chr2.4:6327321-6327340 MS.gene31129:CDS 25.0%
! TAACTCATATGAATCTAACT+GGG - chr2.4:6327068-6327087 MS.gene31129:CDS 25.0%
! TATGTATCTTAGGATGTTAT+TGG + chr2.4:6326845-6326864 None:intergenic 25.0%
! TGATACTTGTAATGAATAAC+AGG - chr2.4:6327320-6327339 MS.gene31129:CDS 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG - chr2.4:6328413-6328432 MS.gene31129:CDS 25.0%
!! TATAAATGTTATAGCTTGTG+AGG - chr2.4:6328412-6328431 MS.gene31129:CDS 25.0%
!!! GCATCTTATAATTTTGATTC+AGG - chr2.4:6327453-6327472 MS.gene31129:CDS 25.0%
!!! TCTTATAATTTTGATTCAGG+AGG - chr2.4:6327456-6327475 MS.gene31129:CDS 25.0%
AACACTAAAATCTTGCAGAA+AGG + chr2.4:6326713-6326732 None:intergenic 30.0%
AGAAAACTTTCATTAAGACC+TGG - chr2.4:6327915-6327934 MS.gene31129:CDS 30.0%
AGATTCATATGAGTTATCAG+TGG + chr2.4:6327064-6327083 None:intergenic 30.0%
ATCTACTACGAAAAATGTTG+CGG - chr2.4:6327515-6327534 MS.gene31129:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG + chr2.4:6328059-6328078 None:intergenic 30.0%
TAAAGGAGTTTGTAGTTGTA+TGG + chr2.4:6327124-6327143 None:intergenic 30.0%
TTAGCTCATTACTTTGCTAA+TGG - chr2.4:6327666-6327685 MS.gene31129:CDS 30.0%
TTTGTATGCTTTATCTGCTT+GGG - chr2.4:6328628-6328647 MS.gene31129:CDS 30.0%
! CACTATTTCGAAAATCGTTT+TGG + chr2.4:6327826-6327845 None:intergenic 30.0%
! CATCGACTACAAAATCATTA+TGG + chr2.4:6328568-6328587 None:intergenic 30.0%
! TAATGATTTTGTAGTCGATG+TGG - chr2.4:6328568-6328587 MS.gene31129:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG - chr2.4:6327683-6327702 MS.gene31129:CDS 30.0%
! TTAATGAAAGTTTTCTGACG+AGG + chr2.4:6327911-6327930 None:intergenic 30.0%
! TTTTGTATGCTTTATCTGCT+TGG - chr2.4:6328627-6328646 MS.gene31129:CDS 30.0%
!! AGATTTTAGTGTTCCAAACT+CGG - chr2.4:6326720-6326739 MS.gene31129:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG - chr2.4:6327858-6327877 MS.gene31129:CDS 30.0%
!!! CTTTTTTCTTGTACTGTTGA+AGG + chr2.4:6327276-6327295 None:intergenic 30.0%
!!! TAATTTTGATTCAGGAGGAA+AGG - chr2.4:6327461-6327480 MS.gene31129:CDS 30.0%
AAACTTTCATTAAGACCTGG+TGG - chr2.4:6327918-6327937 MS.gene31129:CDS 35.0%
AAGTCCTTCTATGATGTTCT+TGG - chr2.4:6326949-6326968 MS.gene31129:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG - chr2.4:6328233-6328252 MS.gene31129:CDS 35.0%
ATACATAAGTGACATCCTCA+TGG - chr2.4:6326858-6326877 MS.gene31129:CDS 35.0%
ATCGCTGTTTGATATGTTTG+AGG - chr2.4:6328325-6328344 MS.gene31129:CDS 35.0%
ATGAGCTACTCAAACAGATT+AGG - chr2.4:6327607-6327626 MS.gene31129:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG + chr2.4:6327948-6327967 None:intergenic 35.0%
CAAAGTAATGAGCTAAACGT+TGG + chr2.4:6327662-6327681 None:intergenic 35.0%
CGGTAAACCAAGTAACAAAT+GGG - chr2.4:6326740-6326759 MS.gene31129:CDS 35.0%
TCGGTAAACCAAGTAACAAA+TGG - chr2.4:6326739-6326758 MS.gene31129:CDS 35.0%
TGTAATGAATAACAGGGAAG+AGG - chr2.4:6327327-6327346 MS.gene31129:CDS 35.0%
TTAAACCGAGTCAGGAAAAA+CGG + chr2.4:6328284-6328303 None:intergenic 35.0%
! ACTCAAACCCATTTGTTACT+TGG + chr2.4:6326750-6326769 None:intergenic 35.0%
! GAATTTTCTATGGTTTCCAG+TGG - chr2.4:6327880-6327899 MS.gene31129:CDS 35.0%
! GGAATATCTTGTCATTTCCA+AGG + chr2.4:6326824-6326843 None:intergenic 35.0%
! GGATGTCACTTATGTATCTT+AGG + chr2.4:6326855-6326874 None:intergenic 35.0%
!! AAATCTTGCAGAAAGGTGTT+TGG + chr2.4:6326706-6326725 None:intergenic 35.0%
!! AATCAGGTTGGATTCTGATT+TGG - chr2.4:6328508-6328527 MS.gene31129:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG + chr2.4:6327556-6327575 None:intergenic 35.0%
!!! GATGGTAAGTGGGTTTTAAA+GGG - chr2.4:6328590-6328609 MS.gene31129:CDS 35.0%
AAACATTCCTGCAACATGCA+TGG + chr2.4:6326910-6326929 None:intergenic 40.0%
AAACCAAGAGGCTGATATGA+TGG + chr2.4:6327720-6327739 None:intergenic 40.0%
AAATCAATGTGCACAAGCTG+TGG - chr2.4:6327560-6327579 MS.gene31129:CDS 40.0%
AACCAAAGCAATGGTGAAGA+AGG - chr2.4:6328538-6328557 MS.gene31129:CDS 40.0%
AAGCTCAAATGGAGAATCCT+TGG - chr2.4:6326804-6326823 MS.gene31129:CDS 40.0%
ACAACTTCATTGCACAGAGA+TGG - chr2.4:6328066-6328085 MS.gene31129:intron 40.0%
AGGATTTAGACCTGCAGAAA+GGG - chr2.4:6327983-6328002 MS.gene31129:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG + chr2.4:6326876-6326895 None:intergenic 40.0%
ATCATATCAGCCTCTTGGTT+TGG - chr2.4:6327719-6327738 MS.gene31129:CDS 40.0%
ATGGCCAAGAACATCATAGA+AGG + chr2.4:6326956-6326975 None:intergenic 40.0%
CAACTTCATTGCACAGAGAT+GGG - chr2.4:6328067-6328086 MS.gene31129:intron 40.0%
CATGGATGAAGAAGATGACT+TGG - chr2.4:6326876-6326895 MS.gene31129:CDS 40.0%
CTATCTTGAGATCCTTGAGA+CGG + chr2.4:6328100-6328119 None:intergenic 40.0%
GCATTTGTCATTCTCTGCAA+AGG + chr2.4:6327795-6327814 None:intergenic 40.0%
GGTGAATGAAACCAAAGCAA+TGG - chr2.4:6328529-6328548 MS.gene31129:CDS 40.0%
GTTACTTGGTTTACCGAGTT+TGG + chr2.4:6326736-6326755 None:intergenic 40.0%
TCAAGGATCTCAAGATAGAC+AGG - chr2.4:6328102-6328121 MS.gene31129:CDS 40.0%
TCATCATCTTCACCTTCTTC+TGG - chr2.4:6326778-6326797 MS.gene31129:CDS 40.0%
TTCAATGCCACAGTTTCATC+AGG + chr2.4:6328173-6328192 None:intergenic 40.0%
TTCCTTCTTCACCATTGCTT+TGG + chr2.4:6328543-6328562 None:intergenic 40.0%
! ACCTCATCTAAAATCTCTGG+TGG + chr2.4:6327390-6327409 None:intergenic 40.0%
! ACTCCATGGAAGAAGACTTT+TGG + chr2.4:6328239-6328258 None:intergenic 40.0%
! AGCACCTCATCTAAAATCTC+TGG + chr2.4:6327393-6327412 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG - chr2.4:6328572-6328591 MS.gene31129:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG - chr2.4:6326918-6326937 MS.gene31129:CDS 40.0%
!! GGATGGTAAGTGGGTTTTAA+AGG - chr2.4:6328589-6328608 MS.gene31129:CDS 40.0%
!!! GCTGCTAGTTTTGAGAGAAA+TGG - chr2.4:6327027-6327046 MS.gene31129:CDS 40.0%
!!! GTAAGTGGGTTTTAAAGGGA+TGG - chr2.4:6328594-6328613 MS.gene31129:CDS 40.0%
!!! GTTTTTCCTGACTCGGTTTA+AGG - chr2.4:6328283-6328302 MS.gene31129:CDS 40.0%
!!! TGGGTTTTAAAGGGATGGAA+AGG - chr2.4:6328599-6328618 MS.gene31129:CDS 40.0%
AACTTCCATTGGCAACTCCA+TGG + chr2.4:6328253-6328272 None:intergenic 45.0%
AAGAGTCGAATTCGACGACA+TGG + chr2.4:6327098-6327117 None:intergenic 45.0%
AAGCTGTGTTGAAGCTGATC+AGG - chr2.4:6328204-6328223 MS.gene31129:CDS 45.0%
ACCATTGTCACAACGGAACT+TGG - chr2.4:6327208-6327227 MS.gene31129:CDS 45.0%
AGCTTCAACACAGCTTCTCT+TGG + chr2.4:6328200-6328219 None:intergenic 45.0%
AGTCGATGTGGATGGTAAGT+GGG - chr2.4:6328580-6328599 MS.gene31129:CDS 45.0%
ATAACAGGGAAGAGGTAGCA+AGG - chr2.4:6327335-6327354 MS.gene31129:CDS 45.0%
ATCCAGTTGTCATCGAGACA+CGG - chr2.4:6327178-6327197 MS.gene31129:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG + chr2.4:6328169-6328188 None:intergenic 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG - chr2.4:6328380-6328399 MS.gene31129:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG - chr2.4:6327986-6328005 MS.gene31129:CDS 45.0%
CACGATCGAAGTTTCCATCT+TGG + chr2.4:6327008-6327027 None:intergenic 45.0%
CAGGATTTAGACCTGCAGAA+AGG - chr2.4:6327982-6328001 MS.gene31129:CDS 45.0%
CATAACCATCCATCCCCAAA+TGG - chr2.4:6326973-6326992 MS.gene31129:CDS 45.0%
CATTTGGGGATGGATGGTTA+TGG + chr2.4:6326975-6326994 None:intergenic 45.0%
CCTCCAAAGCTTCGAATTAC+AGG - chr2.4:6327942-6327961 MS.gene31129:CDS 45.0%
CTTGGTCAGTGCTACCATTT+GGG + chr2.4:6326990-6327009 None:intergenic 45.0%
CTTTCTGCAGGTCTAAATCC+TGG + chr2.4:6327984-6328003 None:intergenic 45.0%
GAACCTTCCTGATGAAACTG+TGG - chr2.4:6328163-6328182 MS.gene31129:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG - chr2.4:6327236-6327255 MS.gene31129:CDS 45.0%
GCAAAAACCATGCATGTTGC+AGG - chr2.4:6326900-6326919 MS.gene31129:CDS 45.0%
GGTGCTAACCATTGTCACAA+CGG - chr2.4:6327201-6327220 MS.gene31129:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG - chr2.4:6328579-6328598 MS.gene31129:CDS 45.0%
TCCAAGTTCCGTTGTGACAA+TGG + chr2.4:6327212-6327231 None:intergenic 45.0%
TCTTCTGGCACAAGCTCAAA+TGG - chr2.4:6326793-6326812 MS.gene31129:CDS 45.0%
TCTTGGTCAGTGCTACCATT+TGG + chr2.4:6326991-6327010 None:intergenic 45.0%
TGAGAGAGGGTTGTTTGGAA+GGG - chr2.4:6328385-6328404 MS.gene31129:CDS 45.0%
TGATTCAGGAGGAAAGGCAA+AGG - chr2.4:6327467-6327486 MS.gene31129:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG - chr2.4:6328496-6328515 MS.gene31129:CDS 45.0%
TTCTTCCATGGAGTTGCCAA+TGG - chr2.4:6328245-6328264 MS.gene31129:CDS 45.0%
TTGAGAGAGGGTTGTTTGGA+AGG - chr2.4:6328384-6328403 MS.gene31129:CDS 45.0%
TTGGTCAGTGCTACCATTTG+GGG + chr2.4:6326989-6327008 None:intergenic 45.0%
TTTGAGCTTGTGCCAGAAGA+AGG + chr2.4:6326793-6326812 None:intergenic 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG + chr2.4:6327945-6327964 None:intergenic 45.0%
! GAAAGTTTTCTGACGAGGCA+CGG + chr2.4:6327906-6327925 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG - chr2.4:6327386-6327405 MS.gene31129:CDS 45.0%
!! AATGGTAGCACTGACCAAGA+TGG - chr2.4:6326991-6327010 MS.gene31129:CDS 45.0%
!! ATGTTGCGGCTGTTGATTTG+TGG - chr2.4:6327529-6327548 MS.gene31129:CDS 45.0%
AAAGGGTGGAGGAAACTGGA+CGG - chr2.4:6328000-6328019 MS.gene31129:intron 50.0%
ACGCCATCATATCAGCCTCT+TGG - chr2.4:6327714-6327733 MS.gene31129:CDS 50.0%
AGAGGTCGATGCTTGAGAGA+GGG - chr2.4:6328372-6328391 MS.gene31129:CDS 50.0%
AGCTTGTGAGGGAGCAGAAA+GGG - chr2.4:6328424-6328443 MS.gene31129:CDS 50.0%
AGGGAGCAGAAAGGGTTGAA+AGG - chr2.4:6328432-6328451 MS.gene31129:CDS 50.0%
AGGTCAGGAACAAGAGAGCT+GGG - chr2.4:6328477-6328496 MS.gene31129:CDS 50.0%
ATGGGAAACCATCCGTCTCA+AGG - chr2.4:6328085-6328104 MS.gene31129:intron 50.0%
CGGTGCGCTATAACTTCCAT+TGG + chr2.4:6328264-6328283 None:intergenic 50.0%
CGTTGGAGTCCATTGCCAAA+AGG + chr2.4:6327645-6327664 None:intergenic 50.0%
CTTGAGATCCTTGAGACGGA+TGG + chr2.4:6328096-6328115 None:intergenic 50.0%
GAAACTTCTTCCACCGTCAC+TGG + chr2.4:6327252-6327271 None:intergenic 50.0%
GAGCTGGGTTCAAGCAAATC+AGG - chr2.4:6328492-6328511 MS.gene31129:CDS 50.0%
GTCACAACGGAACTTGGAGT+TGG - chr2.4:6327214-6327233 MS.gene31129:CDS 50.0%
GTGCTTCCTTAAACCGAGTC+AGG + chr2.4:6328292-6328311 None:intergenic 50.0%
TCAGTGCTACCATTTGGGGA+TGG + chr2.4:6326985-6327004 None:intergenic 50.0%
TCCAGTTGTCATCGAGACAC+GGG - chr2.4:6327179-6327198 MS.gene31129:CDS 50.0%
TGCTACCATTTGGGGATGGA+TGG + chr2.4:6326981-6327000 None:intergenic 50.0%
! AGGCAACACTCTTCGCCTTT+TGG - chr2.4:6327627-6327646 MS.gene31129:CDS 50.0%
! CACTCTTCGCCTTTTGGCAA+TGG - chr2.4:6327633-6327652 MS.gene31129:CDS 50.0%
! TAGCTTGTGAGGGAGCAGAA+AGG - chr2.4:6328423-6328442 MS.gene31129:CDS 50.0%
!! GAGGTGCTGCTTTATCAGAC+AGG - chr2.4:6327405-6327424 MS.gene31129:CDS 50.0%
CAGAGGTCGATGCTTGAGAG+AGG - chr2.4:6328371-6328390 MS.gene31129:CDS 55.0%
CAGGTCAGGAACAAGAGAGC+TGG - chr2.4:6328476-6328495 MS.gene31129:CDS 55.0%
CCAGCAGTTGCCAAACCAAG+AGG + chr2.4:6327732-6327751 None:intergenic 55.0%
CCAGTTGTCATCGAGACACG+GGG - chr2.4:6327180-6327199 MS.gene31129:CDS 55.0%
CTGTGGCGAGCTACGATCAA+AGG - chr2.4:6327577-6327596 MS.gene31129:CDS 55.0%
GCAGAAAGGGTGGAGGAAAC+TGG - chr2.4:6327996-6328015 MS.gene31129:intron 55.0%
GCAGGTCTAAATCCTGGCTG+AGG + chr2.4:6327978-6327997 None:intergenic 55.0%
GGAATTGAGCTTCCTCAGCC+AGG - chr2.4:6327963-6327982 MS.gene31129:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG + chr2.4:6327996-6328015 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG - chr2.4:6327989-6328008 MS.gene31129:CDS 55.0%
TGACATTCAGCCAGTGACGG+TGG - chr2.4:6327239-6327258 MS.gene31129:CDS 55.0%
TGAGACGTACAAGCAGTGGC+AGG - chr2.4:6328457-6328476 MS.gene31129:CDS 55.0%
TGCCACTGCTTGTACGTCTC+AGG + chr2.4:6328458-6328477 None:intergenic 55.0%
!! CCTCTTGGTTTGGCAACTGC+TGG - chr2.4:6327729-6327748 MS.gene31129:CDS 55.0%
!! GCGCACCGTTTTTCCTGACT+CGG - chr2.4:6328276-6328295 MS.gene31129:CDS 55.0%
CCCCGTGTCTCGATGACAAC+TGG + chr2.4:6327183-6327202 None:intergenic 60.0%
CGTACAAGCAGTGGCAGGTC+AGG - chr2.4:6328462-6328481 MS.gene31129:CDS 60.0%
GACCTCTGCGTGTCTTCACG+AGG + chr2.4:6328359-6328378 None:intergenic 60.0%
GGCCTGAGACGTACAAGCAG+TGG - chr2.4:6328453-6328472 MS.gene31129:CDS 60.0%
TGCCTCGTGAAGACACGCAG+AGG - chr2.4:6328354-6328373 MS.gene31129:CDS 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG + chr2.4:6327936-6327955 None:intergenic 60.0%
TTCTGACGAGGCACGGCCAC+TGG + chr2.4:6327899-6327918 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6326694 6328661 6326694 ID=MS.gene31129
chr2.4 mRNA 6326694 6328661 6326694 ID=MS.gene31129.t1;Parent=MS.gene31129
chr2.4 exon 6328098 6328661 6328098 ID=MS.gene31129.t1.exon1;Parent=MS.gene31129.t1
chr2.4 CDS 6328098 6328661 6328098 ID=cds.MS.gene31129.t1;Parent=MS.gene31129.t1
chr2.4 exon 6326694 6328013 6326694 ID=MS.gene31129.t1.exon2;Parent=MS.gene31129.t1
chr2.4 CDS 6326694 6328013 6326694 ID=cds.MS.gene31129.t1;Parent=MS.gene31129.t1
Gene Sequence

>MS.gene31129

ATGCTTTCTCCAAACACCTTTCTGCAAGATTTTAGTGTTCCAAACTCGGTAAACCAAGTAACAAATGGGTTTGAGTTAGAAGATTCATCATCTTCACCTTCTTCTGGCACAAGCTCAAATGGAGAATCCTTGGAAATGACAAGATATTCCAATAACATCCTAAGATACATAAGTGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAAACCATGCATGTTGCAGGAATGTTTAAGACTTCAGGCTGCTGAAAAGTCCTTCTATGATGTTCTTGGCCATAACCATCCATCCCCAAATGGTAGCACTGACCAAGATGGAAACTTCGATCGTGCTGCTAGTTTTGAGAGAAATGGCAGCAGCTGCACCACTGATAACTCATATGAATCTAACTGGGTCAACCATGTCGTCGAATTCGACTCTTCTTCCATACAACTACAAACTCCTTTAATAGAAAAAAACTACTATGACTTGACAGAAGCTGATCCAGTTGTCATCGAGACACGGGGTGCTAACCATTGTCACAACGGAACTTGGAGTTGGAATGACATTCAGCCAGTGACGGTGGAAGAAGTTTCACCTTCAACAGTACAAGAAAAAAGAAGTCATAAGATGGATAATGATAATGATACTTGTAATGAATAACAGGGAAGAGGTAGCAAGGTTTCAGCTATTTTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTGCTGCTTTATCAGACAGGAAAGACTCTCACTCAACACCAACAAGCATCTTATAATTTTGATTCAGGAGGAAAGGCAAAGGAAACACATTCGCGTTTGAAGAAAGCATCTACTACGAAAAATGTTGCGGCTGTTGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTCGCCTTTTGGCAATGGACTCCAACGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACGCCATCATATCAGCCTCTTGGTTTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACTGCGTCTCCTTTGCAGAGAATGACAAATGCTTTGCTAACCAAAACGATTTTCGAAATAGTGAAGAACGAGAGCAGCGTTCATGTTATTGATTTTGGAATTTTCTATGGTTTCCAGTGGCCGTGCCTCGTCAGAAAACTTTCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCAGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACTGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAGATGGGAAACCATCCGTCTCAAGGATCTCAAGATAGACAGGAATGAAATAACTCTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGCTGTGTTGAAGCTGATCAGGAAGATTAATCCAAAAGTCTTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCTGACTCGGTTTAAGGAAGCACTCTATCACTTCTCATCGCTGTTTGATATGTTTGAGGCTAACGTGCCTCGTGAAGACACGCAGAGGTCGATGCTTGAGAGAGGGTTGTTTGGAAGGGATGCTATAAATGTTATAGCTTGTGAGGGAGCAGAAAGGGTTGAAAGGCCTGAGACGTACAAGCAGTGGCAGGTCAGGAACAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTGGTGAATGAAACCAAAGCAATGGTGAAGAAGGAATACCATAATGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTAAAGGGATGGAAAGGACGCATTTTGTATGCTTTATCTGCTTGGGTTCCTGCTTAA
Protein sequence

>MS.gene31129.t1

MLSPNTFLQDFSVPNSVNQVTNGFELEDSSSSPSSGTSSNGESLEMTRYSNNILRYISDILMDEEDDLEQKPCMLQECLRLQAAEKSFYDVLGHNHPSPNGSTDQDGNFDRAASFERNGSSCTTDNSYESNWVNHVVEFDSSSIQLQTPLIEKNYYDLTEADPVVIETRGANHCHNGTWSWNDIQPVTGRGSKVSAIFSDELEPPEILDEVLLYQTGKTLTQHQQASYNFDSGGKAKETHSRLKKASTTKNVAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSSPFGNGLQRLAHYFANGLEIRLAAETPSYQPLGLATAGDMLKAYKLFVTASPLQRMTNALLTKTIFEIVKNESSVHVIDFGIFYGFQWPCLVRKLSLRPGGPPKLRITGIELPQPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQRWETIRLKDLKIDRNEITLVSCLYRLKNLPDETVALNCPREAVLKLIRKINPKVFFHGVANGSYSAPFFLTRFKEALYHFSSLFDMFEANVPREDTQRSMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKAMVKKEYHNDFVVDVDGKWVLKGWKGRILYALSAWVPA