Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31350.t1 | XP_003597127.2 | 100 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 3.60E-92 | 347.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31350.t1 | Q9S7R3 | 86.0 | 143 | 19 | 1 | 5 | 147 | 8 | 149 | 1.6e-69 | 263.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31350.t1 | G7IU28 | 100.0 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 2.6e-92 | 347.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051648 | MS.gene31350 | 0.825071 | 5.72E-54 | -1.69E-46 |
| MS.gene052150 | MS.gene31350 | 0.802176 | 6.35E-49 | -1.69E-46 |
| MS.gene052151 | MS.gene31350 | 0.810441 | 1.15E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31350.t1 | MTR_2g092950 | 100.000 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 2.84e-126 | 351 |
| MS.gene31350.t1 | MTR_4g079930 | 86.713 | 143 | 18 | 1 | 5 | 146 | 10 | 152 | 1.14e-93 | 269 |
| MS.gene31350.t1 | MTR_2g076400 | 78.846 | 156 | 23 | 1 | 1 | 146 | 1 | 156 | 1.69e-89 | 259 |
| MS.gene31350.t1 | MTR_2g016110 | 76.433 | 157 | 32 | 2 | 11 | 162 | 20 | 176 | 5.81e-83 | 243 |
| MS.gene31350.t1 | MTR_4g094428 | 70.345 | 145 | 42 | 1 | 21 | 164 | 44 | 188 | 7.23e-73 | 219 |
| MS.gene31350.t1 | MTR_5g072510 | 71.970 | 132 | 37 | 0 | 20 | 151 | 34 | 165 | 1.04e-72 | 218 |
| MS.gene31350.t1 | MTR_8g086350 | 83.333 | 126 | 21 | 0 | 21 | 146 | 29 | 154 | 7.12e-70 | 209 |
| MS.gene31350.t1 | MTR_1g442860 | 76.800 | 125 | 29 | 0 | 23 | 147 | 65 | 189 | 7.82e-70 | 210 |
| MS.gene31350.t1 | MTR_7g115700 | 65.306 | 147 | 46 | 3 | 12 | 155 | 16 | 160 | 4.72e-66 | 199 |
| MS.gene31350.t1 | MTR_1g075990 | 76.515 | 132 | 31 | 0 | 20 | 151 | 50 | 181 | 7.77e-66 | 199 |
| MS.gene31350.t1 | MTR_7g097030 | 77.692 | 130 | 29 | 0 | 22 | 151 | 63 | 192 | 5.90e-64 | 196 |
| MS.gene31350.t1 | MTR_3g031830 | 71.127 | 142 | 41 | 0 | 10 | 151 | 29 | 170 | 7.16e-64 | 196 |
| MS.gene31350.t1 | MTR_1g069825 | 77.519 | 129 | 29 | 0 | 23 | 151 | 52 | 180 | 2.28e-63 | 194 |
| MS.gene31350.t1 | MTR_1g080210 | 73.600 | 125 | 33 | 0 | 23 | 147 | 40 | 164 | 1.89e-58 | 180 |
| MS.gene31350.t1 | MTR_1g114020 | 63.636 | 55 | 17 | 1 | 12 | 66 | 63 | 114 | 6.25e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31350.t1 | AT2G42610 | 85.915 | 142 | 19 | 1 | 5 | 146 | 8 | 148 | 1.41e-89 | 259 |
| MS.gene31350.t1 | AT2G42610 | 85.915 | 142 | 19 | 1 | 5 | 146 | 8 | 148 | 1.41e-89 | 259 |
| MS.gene31350.t1 | AT1G07090 | 71.533 | 137 | 39 | 0 | 15 | 151 | 23 | 159 | 6.81e-74 | 220 |
| MS.gene31350.t1 | AT1G78815 | 75.591 | 127 | 31 | 0 | 20 | 146 | 37 | 163 | 5.62e-73 | 218 |
| MS.gene31350.t1 | AT4G18610 | 76.984 | 126 | 28 | 1 | 22 | 146 | 36 | 161 | 2.51e-71 | 213 |
| MS.gene31350.t1 | AT5G58500 | 74.046 | 131 | 33 | 1 | 22 | 151 | 18 | 148 | 6.16e-71 | 212 |
| MS.gene31350.t1 | AT1G16910 | 68.531 | 143 | 45 | 0 | 20 | 162 | 20 | 162 | 2.24e-70 | 210 |
| MS.gene31350.t1 | AT2G31160 | 76.744 | 129 | 30 | 0 | 23 | 151 | 54 | 182 | 2.08e-64 | 197 |
| MS.gene31350.t1 | AT3G23290 | 77.953 | 127 | 28 | 0 | 21 | 147 | 46 | 172 | 1.30e-63 | 194 |
| MS.gene31350.t1 | AT5G28490 | 74.627 | 134 | 34 | 0 | 22 | 155 | 24 | 157 | 1.89e-63 | 193 |
| MS.gene31350.t1 | AT3G04510 | 71.970 | 132 | 37 | 0 | 23 | 154 | 33 | 164 | 4.34e-62 | 190 |
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAGGTATTTAGATCAATT+TGG | 0.234689 | 2.4:-8863193 | MS.gene31350:CDS |
| ACCATGTGTTGGAATTTCTT+AGG | 0.249818 | 2.4:-8863210 | MS.gene31350:CDS |
| GGAAGCCTTGATGCTCTTAT+TGG | 0.251412 | 2.4:-8863082 | MS.gene31350:CDS |
| ATGCTCTTATTGGGAGGTTA+AGG | 0.286320 | 2.4:-8863072 | MS.gene31350:CDS |
| AGAAGAGATTGGAACACTTT+TGG | 0.305989 | 2.4:-8863286 | MS.gene31350:CDS |
| GAAGCCTTGATGCTCTTATT+GGG | 0.358150 | 2.4:-8863081 | MS.gene31350:CDS |
| TGCTCTTATTGGGAGGTTAA+GGG | 0.383201 | 2.4:-8863071 | MS.gene31350:CDS |
| CGTTTATCTTAGAGAGGTTA+GGG | 0.392703 | 2.4:-8862987 | MS.gene31350:CDS |
| GTACAAGGTGCTGGTGGCTC+TGG | 0.394151 | 2.4:+8863127 | None:intergenic |
| GCGTTTATCTTAGAGAGGTT+AGG | 0.401453 | 2.4:-8862988 | MS.gene31350:CDS |
| ATGTCCACTTAGACAAGCTT+GGG | 0.408374 | 2.4:-8863104 | MS.gene31350:CDS |
| AAAGTGGAACGGGTGGTCTA+AGG | 0.420057 | 2.4:+8863248 | None:intergenic |
| AATCTCAAAAGAGAAGAGAT+TGG | 0.439992 | 2.4:-8863297 | MS.gene31350:CDS |
| ACAACCTTGTAAGTGAACTT+TGG | 0.477909 | 2.4:+8863165 | None:intergenic |
| TTGCAGTGAGAAAGTGGAAC+GGG | 0.495114 | 2.4:+8863238 | None:intergenic |
| CTTCCCCAAGCTTGTCTAAG+TGG | 0.503711 | 2.4:+8863100 | None:intergenic |
| CATGTCCACTTAGACAAGCT+TGG | 0.511810 | 2.4:-8863105 | MS.gene31350:CDS |
| AAAACCAAAGTTCACTTACA+AGG | 0.520249 | 2.4:-8863169 | MS.gene31350:CDS |
| GGGAGTGTCAAGCTAAGGCT+AGG | 0.530004 | 2.4:-8862967 | MS.gene31350:CDS |
| AAACCAAATATGTCTTCAAG+TGG | 0.542706 | 2.4:-8863379 | None:intergenic |
| AAGAAATTCCAACACATGGT+TGG | 0.545252 | 2.4:+8863213 | None:intergenic |
| GAGGTTAAGGGCTGCATATG+AGG | 0.551450 | 2.4:-8863059 | MS.gene31350:CDS |
| TGTCTAAGTGGACATGTACA+AGG | 0.553070 | 2.4:+8863112 | None:intergenic |
| TGAGGAAAACGGTGGAACTC+CGG | 0.553996 | 2.4:-8863041 | MS.gene31350:CDS |
| CTGCAATTCCAACCATGTGT+TGG | 0.557019 | 2.4:-8863221 | MS.gene31350:CDS |
| AGTGGAAAGGATTTAGCTGA+AGG | 0.563487 | 2.4:-8863361 | MS.gene31350:CDS |
| GGTTAGGGAGTGTCAAGCTA+AGG | 0.565244 | 2.4:-8862972 | MS.gene31350:CDS |
| GGAGTGTCAAGCTAAGGCTA+GGG | 0.568004 | 2.4:-8862966 | MS.gene31350:CDS |
| AAATATGTCTTCAAGTGGAA+AGG | 0.571184 | 2.4:-8863374 | None:intergenic |
| CTCGCGAAAGGATTAGTCTC+CGG | 0.573225 | 2.4:+8863022 | None:intergenic |
| AGGGCTGCATATGAGGAAAA+CGG | 0.576049 | 2.4:-8863052 | MS.gene31350:CDS |
| CGAATAGCACCGCTCGCGAA+AGG | 0.579160 | 2.4:+8863010 | None:intergenic |
| TTGGAATTGCAGTGAGAAAG+TGG | 0.579943 | 2.4:+8863232 | None:intergenic |
| GGAGGATCATCAACCTCGCA+AGG | 0.584784 | 2.4:-8863340 | MS.gene31350:CDS |
| GCCTTGATGCTCTTATTGGG+AGG | 0.590380 | 2.4:-8863078 | MS.gene31350:CDS |
| GCTAAGTGTGATTCCTTGCG+AGG | 0.590859 | 2.4:+8863327 | None:intergenic |
| ACCTCCCAATAAGAGCATCA+AGG | 0.595384 | 2.4:+8863077 | None:intergenic |
| AGTGGACATGTACAAGGTGC+TGG | 0.597857 | 2.4:+8863118 | None:intergenic |
| TATTCGCGTTTATCTTAGAG+AGG | 0.607515 | 2.4:-8862993 | MS.gene31350:CDS |
| ACCTAAGAAATTCCAACACA+TGG | 0.612951 | 2.4:+8863209 | None:intergenic |
| GGAAATTCAAGAAAAGTGCA+TGG | 0.626006 | 2.4:+8862883 | None:intergenic |
| GGAAAGGATTTAGCTGAAGG+AGG | 0.626283 | 2.4:-8863358 | MS.gene31350:CDS |
| GAGACTAATCCTTTCGCGAG+CGG | 0.631568 | 2.4:-8863019 | MS.gene31350:CDS |
| CAGTGAGAAAGTGGAACGGG+TGG | 0.652107 | 2.4:+8863241 | None:intergenic |
| GAGTGTCAAGCTAAGGCTAG+GGG | 0.673844 | 2.4:-8862965 | MS.gene31350:CDS |
| GGACATGTACAAGGTGCTGG+TGG | 0.708995 | 2.4:+8863121 | None:intergenic |
| TGTCCACTTAGACAAGCTTG+GGG | 0.716611 | 2.4:-8863103 | MS.gene31350:CDS |
| ATTGCAGTGAGAAAGTGGAA+CGG | 0.719471 | 2.4:+8863237 | None:intergenic |
| GCTGCATATGAGGAAAACGG+TGG | 0.735412 | 2.4:-8863049 | MS.gene31350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTAGGTATTTAGATCAATT+TGG | - | chr2.4:8863066-8863085 | MS.gene31350:CDS | 25.0% |
| !! | AAAAAGAAGAGCACAAATAA+GGG | - | chr2.4:8863333-8863352 | MS.gene31350:CDS | 25.0% |
| !! | TCTTCTTTTTCTTCTTGTAA+GGG | + | chr2.4:8863323-8863342 | None:intergenic | 25.0% |
| !! | TTACAAGGTTGTATGTTTTA+TGG | - | chr2.4:8863105-8863124 | MS.gene31350:CDS | 25.0% |
| AAAACCAAAGTTCACTTACA+AGG | - | chr2.4:8863090-8863109 | MS.gene31350:CDS | 30.0% | |
| AATCTCAAAAGAGAAGAGAT+TGG | - | chr2.4:8862962-8862981 | MS.gene31350:CDS | 30.0% | |
| ! | CTCTTCTTTTTCTTCTTGTA+AGG | + | chr2.4:8863324-8863343 | None:intergenic | 30.0% |
| ! | GAAAAAGAAGAGCACAAATA+AGG | - | chr2.4:8863332-8863351 | MS.gene31350:CDS | 30.0% |
| !!! | TTCTCTTTTGAGATTCATAG+CGG | + | chr2.4:8862957-8862976 | None:intergenic | 30.0% |
| AAGAAATTCCAACACATGGT+TGG | + | chr2.4:8863049-8863068 | None:intergenic | 35.0% | |
| ACAACCTTGTAAGTGAACTT+TGG | + | chr2.4:8863097-8863116 | None:intergenic | 35.0% | |
| ACCATGTGTTGGAATTTCTT+AGG | - | chr2.4:8863049-8863068 | MS.gene31350:CDS | 35.0% | |
| ACCTAAGAAATTCCAACACA+TGG | + | chr2.4:8863053-8863072 | None:intergenic | 35.0% | |
| CGTTTATCTTAGAGAGGTTA+GGG | - | chr2.4:8863272-8863291 | MS.gene31350:CDS | 35.0% | |
| TATTCGCGTTTATCTTAGAG+AGG | - | chr2.4:8863266-8863285 | MS.gene31350:CDS | 35.0% | |
| ! | AGAAGAGATTGGAACACTTT+TGG | - | chr2.4:8862973-8862992 | MS.gene31350:CDS | 35.0% |
| AGTGGAAAGGATTTAGCTGA+AGG | - | chr2.4:8862898-8862917 | MS.gene31350:CDS | 40.0% | |
| ATGCTCTTATTGGGAGGTTA+AGG | - | chr2.4:8863187-8863206 | MS.gene31350:CDS | 40.0% | |
| ATGTCCACTTAGACAAGCTT+GGG | - | chr2.4:8863155-8863174 | MS.gene31350:CDS | 40.0% | |
| ATTGCAGTGAGAAAGTGGAA+CGG | + | chr2.4:8863025-8863044 | None:intergenic | 40.0% | |
| GAAGCCTTGATGCTCTTATT+GGG | - | chr2.4:8863178-8863197 | MS.gene31350:CDS | 40.0% | |
| GCGTTTATCTTAGAGAGGTT+AGG | - | chr2.4:8863271-8863290 | MS.gene31350:CDS | 40.0% | |
| TGCTCTTATTGGGAGGTTAA+GGG | - | chr2.4:8863188-8863207 | MS.gene31350:CDS | 40.0% | |
| TGTCTAAGTGGACATGTACA+AGG | + | chr2.4:8863150-8863169 | None:intergenic | 40.0% | |
| TTGGAATTGCAGTGAGAAAG+TGG | + | chr2.4:8863030-8863049 | None:intergenic | 40.0% | |
| AGGGCTGCATATGAGGAAAA+CGG | - | chr2.4:8863207-8863226 | MS.gene31350:CDS | 45.0% | |
| CATGTCCACTTAGACAAGCT+TGG | - | chr2.4:8863154-8863173 | MS.gene31350:CDS | 45.0% | |
| CTGCAATTCCAACCATGTGT+TGG | - | chr2.4:8863038-8863057 | MS.gene31350:CDS | 45.0% | |
| GGAAAGGATTTAGCTGAAGG+AGG | - | chr2.4:8862901-8862920 | MS.gene31350:CDS | 45.0% | |
| GGAAGCCTTGATGCTCTTAT+TGG | - | chr2.4:8863177-8863196 | MS.gene31350:CDS | 45.0% | |
| TGTCCACTTAGACAAGCTTG+GGG | - | chr2.4:8863156-8863175 | MS.gene31350:CDS | 45.0% | |
| TTGCAGTGAGAAAGTGGAAC+GGG | + | chr2.4:8863024-8863043 | None:intergenic | 45.0% | |
| !! | ACCTCCCAATAAGAGCATCA+AGG | + | chr2.4:8863185-8863204 | None:intergenic | 45.0% |
| AAAGTGGAACGGGTGGTCTA+AGG | + | chr2.4:8863014-8863033 | None:intergenic | 50.0% | |
| AGTGGACATGTACAAGGTGC+TGG | + | chr2.4:8863144-8863163 | None:intergenic | 50.0% | |
| CTTCCCCAAGCTTGTCTAAG+TGG | + | chr2.4:8863162-8863181 | None:intergenic | 50.0% | |
| GAGACTAATCCTTTCGCGAG+CGG | - | chr2.4:8863240-8863259 | MS.gene31350:CDS | 50.0% | |
| GAGGTTAAGGGCTGCATATG+AGG | - | chr2.4:8863200-8863219 | MS.gene31350:CDS | 50.0% | |
| GAGTGTCAAGCTAAGGCTAG+GGG | - | chr2.4:8863294-8863313 | MS.gene31350:CDS | 50.0% | |
| GCCTTGATGCTCTTATTGGG+AGG | - | chr2.4:8863181-8863200 | MS.gene31350:CDS | 50.0% | |
| GCTAAGTGTGATTCCTTGCG+AGG | + | chr2.4:8862935-8862954 | None:intergenic | 50.0% | |
| GCTGCATATGAGGAAAACGG+TGG | - | chr2.4:8863210-8863229 | MS.gene31350:CDS | 50.0% | |
| TGAGGAAAACGGTGGAACTC+CGG | - | chr2.4:8863218-8863237 | MS.gene31350:CDS | 50.0% | |
| ! | GGAGTGTCAAGCTAAGGCTA+GGG | - | chr2.4:8863293-8863312 | MS.gene31350:CDS | 50.0% |
| !! | CTCGCGAAAGGATTAGTCTC+CGG | + | chr2.4:8863240-8863259 | None:intergenic | 50.0% |
| !! | GGTTAGGGAGTGTCAAGCTA+AGG | - | chr2.4:8863287-8863306 | MS.gene31350:CDS | 50.0% |
| CAGTGAGAAAGTGGAACGGG+TGG | + | chr2.4:8863021-8863040 | None:intergenic | 55.0% | |
| GGAGGATCATCAACCTCGCA+AGG | - | chr2.4:8862919-8862938 | MS.gene31350:CDS | 55.0% | |
| !! | GGACATGTACAAGGTGCTGG+TGG | + | chr2.4:8863141-8863160 | None:intergenic | 55.0% |
| !! | GGGAGTGTCAAGCTAAGGCT+AGG | - | chr2.4:8863292-8863311 | MS.gene31350:CDS | 55.0% |
| CGAATAGCACCGCTCGCGAA+AGG | + | chr2.4:8863252-8863271 | None:intergenic | 60.0% | |
| !! | GTACAAGGTGCTGGTGGCTC+TGG | + | chr2.4:8863135-8863154 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 8862889 | 8863392 | 8862889 | ID=MS.gene31350 |
| chr2.4 | mRNA | 8862889 | 8863392 | 8862889 | ID=MS.gene31350.t1;Parent=MS.gene31350 |
| chr2.4 | exon | 8862889 | 8863392 | 8862889 | ID=MS.gene31350.t1.exon1;Parent=MS.gene31350.t1 |
| chr2.4 | CDS | 8862889 | 8863392 | 8862889 | ID=cds.MS.gene31350.t1;Parent=MS.gene31350.t1 |
| Gene Sequence |
| Protein sequence |
