AlfalfaGEDB | MS.gene052151

M. sativa cultivar XinJiangDaYe / MS.gene052151

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03790.16 PF03791.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052151.t1	ABO33479.1	99.1	111	1	0	20	130	25	135	2.60E-53	218

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052151.t1	O04135	83.2	113	15	1	21	129	130	242	1.1e-44	180.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052151.t1	X2D315	99.1	111	1	0	20	130	116	226	1.9e-53	218.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049418	MS.gene052151	0.800713	1.27E-48	-1.69E-46
MS.gene049660	MS.gene052151	0.811161	8.04E-51	-1.69E-46
MS.gene05054	MS.gene052151	0.800732	1.26E-48	-1.69E-46
MS.gene051648	MS.gene052151	0.938608	5.08E-99	-1.69E-46
MS.gene052150	MS.gene052151	0.975066	2.82E-139	-1.69E-46
MS.gene052151	MS.gene053536	0.818349	2.05E-52	-1.69E-46
MS.gene052151	MS.gene053538	0.809005	2.34E-50	-1.69E-46
MS.gene052151	MS.gene056169	0.805986	1.02E-49	-1.69E-46
MS.gene052151	MS.gene057082	0.812886	3.38E-51	-1.69E-46
MS.gene052151	MS.gene05894	0.83381	4.31E-56	-1.69E-46
MS.gene052151	MS.gene059424	0.81151	6.75E-51	-1.69E-46
MS.gene052151	MS.gene061773	0.822115	2.81E-53	-1.69E-46
MS.gene052151	MS.gene061774	0.813603	2.35E-51	-1.69E-46
MS.gene052151	MS.gene061775	0.801689	8.00E-49	-1.69E-46
MS.gene052151	MS.gene065643	0.821529	3.85E-53	-1.69E-46
MS.gene052151	MS.gene065674	0.827734	1.33E-54	-1.69E-46
MS.gene052151	MS.gene066010	0.84314	1.68E-58	-1.69E-46
MS.gene052151	MS.gene069852	0.822346	2.49E-53	-1.69E-46
MS.gene052151	MS.gene071428	0.800038	1.74E-48	-1.69E-46
MS.gene052151	MS.gene07723	0.817201	3.73E-52	-1.69E-46
MS.gene052151	MS.gene09947	0.801428	9.05E-49	-1.69E-46
MS.gene052151	MS.gene21014	0.81126	7.65E-51	-1.69E-46
MS.gene052151	MS.gene21026	0.825936	3.57E-54	-1.69E-46
MS.gene052151	MS.gene21144	0.80177	7.70E-49	-1.69E-46
MS.gene052151	MS.gene24527	0.936266	2.28E-97	-1.69E-46
MS.gene052151	MS.gene24528	0.87417	8.33E-68	-1.69E-46
MS.gene052151	MS.gene26387	0.805505	1.29E-49	-1.69E-46
MS.gene052151	MS.gene26464	0.81778	2.76E-52	-1.69E-46
MS.gene052151	MS.gene26465	0.816769	4.66E-52	-1.69E-46
MS.gene052151	MS.gene26855	0.817032	4.07E-52	-1.69E-46
MS.gene052151	MS.gene27274	0.818113	2.32E-52	-1.69E-46
MS.gene052151	MS.gene27546	0.802652	5.07E-49	-1.69E-46
MS.gene052151	MS.gene28281	0.802893	4.52E-49	-1.69E-46
MS.gene052151	MS.gene31350	0.810441	1.15E-50	-1.69E-46
MS.gene052151	MS.gene32854	0.842096	3.19E-58	-1.69E-46
MS.gene052151	MS.gene32920	0.805416	1.35E-49	-1.69E-46
MS.gene052151	MS.gene35441	0.807215	5.63E-50	-1.69E-46
MS.gene052151	MS.gene37460	0.826202	3.09E-54	-1.69E-46
MS.gene052151	MS.gene38101	0.816546	5.23E-52	-1.69E-46
MS.gene052151	MS.gene38688	0.821927	3.11E-53	-1.69E-46
MS.gene052151	MS.gene40787	0.822762	1.99E-53	-1.69E-46
MS.gene052151	MS.gene41651	0.800294	1.54E-48	-1.69E-46
MS.gene052151	MS.gene43171	0.812389	4.34E-51	-1.69E-46
MS.gene052151	MS.gene43516	0.808184	3.51E-50	-1.69E-46
MS.gene052151	MS.gene44340	0.813113	3.02E-51	-1.69E-46
MS.gene052151	MS.gene44341	0.811626	6.37E-51	-1.69E-46
MS.gene052151	MS.gene45937	0.824606	7.37E-54	-1.69E-46
MS.gene052151	MS.gene49973	0.811919	5.50E-51	-1.69E-46
MS.gene052151	MS.gene50080	0.804781	1.83E-49	-1.69E-46
MS.gene052151	MS.gene52996	0.826394	2.78E-54	-1.69E-46
MS.gene052151	MS.gene53451	0.806456	8.15E-50	-1.69E-46
MS.gene052151	MS.gene54825	0.830405	2.99E-55	-1.69E-46
MS.gene052151	MS.gene55101	0.826902	2.10E-54	-1.69E-46
MS.gene052151	MS.gene57665	0.844409	7.70E-59	-1.69E-46
MS.gene052151	MS.gene59849	0.817892	2.60E-52	-1.69E-46
MS.gene052151	MS.gene64442	0.810197	1.30E-50	-1.69E-46
MS.gene052151	MS.gene65139	0.817146	3.84E-52	-1.69E-46
MS.gene052151	MS.gene65451	0.800945	1.14E-48	-1.69E-46
MS.gene052151	MS.gene71698	0.816092	6.61E-52	-1.69E-46
MS.gene052151	MS.gene72487	0.847154	1.38E-59	-1.69E-46
MS.gene052151	MS.gene76518	0.811449	6.96E-51	-1.69E-46
MS.gene052151	MS.gene77669	0.803958	2.72E-49	-1.69E-46
MS.gene052151	MS.gene77734	0.841259	5.30E-58	-1.69E-46
MS.gene052151	MS.gene78065	0.831331	1.77E-55	-1.69E-46
MS.gene052151	MS.gene79784	0.835196	1.93E-56	-1.69E-46
MS.gene052151	MS.gene79951	0.822971	1.78E-53	-1.69E-46
MS.gene052151	MS.gene80724	0.814382	1.58E-51	-1.69E-46
MS.gene052151	MS.gene82094	0.819779	9.71E-53	-1.69E-46
MS.gene052151	MS.gene84298	0.819595	1.07E-52	-1.69E-46
MS.gene052151	MS.gene84730	0.809151	2.18E-50	-1.69E-46
MS.gene052151	MS.gene84863	0.803894	2.80E-49	-1.69E-46
MS.gene052151	MS.gene85133	0.811879	5.61E-51	-1.69E-46
MS.gene052151	MS.gene90591	0.805076	1.59E-49	-1.69E-46
MS.gene052151	MS.gene92541	0.808045	3.76E-50	-1.69E-46
MS.gene052151	MS.gene92988	0.820158	7.95E-53	-1.69E-46
MS.gene052151	MS.gene96315	0.83011	3.53E-55	-1.69E-46
MS.gene052151	MS.gene99878	0.806287	8.85E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052151.t1	MTR_1g017080	97.692	130	3	0	1	130	97	226	6.09e-91	268
MS.gene052151.t1	MTR_5g033720	55.882	102	45	0	23	124	72	173	1.22e-34	122
MS.gene052151.t1	MTR_2g024390	51.515	99	48	0	23	121	117	215	5.30e-29	108
MS.gene052151.t1	MTR_5g085860	49.020	102	48	1	24	121	47	148	2.19e-24	95.1
MS.gene052151.t1	MTR_1g084060	44.118	102	53	1	23	124	19	116	2.94e-24	94.0
MS.gene052151.t1	MTR_1g084060	44.118	102	53	1	23	124	19	116	5.57e-24	94.0
MS.gene052151.t1	MTR_6g071190	29.915	117	76	1	5	121	24	134	1.96e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052151.t1	AT4G08150	65.354	127	43	1	4	129	115	241	8.63e-53	171
MS.gene052151.t1	AT1G23380	51.456	103	50	0	22	124	84	186	3.70e-31	113
MS.gene052151.t1	AT1G62360	50.833	120	57	1	1	118	97	216	5.65e-30	111
MS.gene052151.t1	AT1G23380	50.476	105	50	1	22	124	84	188	3.04e-29	108
MS.gene052151.t1	AT1G70510	48.077	104	54	0	22	125	68	171	2.75e-28	105
MS.gene052151.t1	AT1G70510	46.296	108	54	1	22	125	68	175	4.47e-26	99.8

Find 31 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAGGATGAGCAATGATTT+TGG	0.278077	1.2:-5686593	None:intergenic
ACCATGAATTGGTCTAGTTC+TGG	0.338115	1.2:-5687031	None:intergenic
ACCAAACGAGCCACCACTTC+CGG	0.349272	1.2:-5686941	None:intergenic
ACCGGAAGTGGTGGCTCGTT+TGG	0.365361	1.2:+5686940	MS.gene052151:CDS
AAATCCATAGCCTCTTGTAT+AGG	0.443002	1.2:-5687299	None:intergenic
TCCAGAACTAGACCAATTCA+TGG	0.475218	1.2:+5687030	MS.gene052151:CDS
CACTTTGCAATGGACCCCTT+CGG	0.484187	1.2:+5687351	MS.gene052151:CDS
GATTGGAGCTCCACCGGAAG+TGG	0.502488	1.2:+5686928	MS.gene052151:intron
GGACCCCTTCGGATCTTCCC+TGG	0.503797	1.2:+5687362	MS.gene052151:CDS
AAGGCCTATACAAGAGGCTA+TGG	0.514686	1.2:+5687295	MS.gene052151:CDS
TGGCTCGTTTGGTTGCGTCT+AGG	0.518848	1.2:+5686951	MS.gene052151:CDS
AGCTTACATGGATTGTCAAA+AGG	0.519024	1.2:+5686633	MS.gene052151:CDS
CATCATCATCAAGGAGGAGG+AGG	0.530576	1.2:+5686529	MS.gene052151:CDS
GCTTCACCACTAGAACTGCT+TGG	0.545490	1.2:-5686556	None:intergenic
TATGTAGATTGGAGCTCCAC+CGG	0.547316	1.2:+5686922	MS.gene052151:intron
ATGCATAATCATCATCATCA+AGG	0.555030	1.2:+5686520	MS.gene052151:CDS
CAGCTAAATACACTTTGCAA+TGG	0.562110	1.2:+5687341	MS.gene052151:CDS
TGGAGCTCCACCGGAAGTGG+TGG	0.570424	1.2:+5686931	MS.gene052151:CDS
TGTAAAAGACTAGAGTATTG+AGG	0.573212	1.2:-5686610	None:intergenic
GGGAGTCCAAGCAGTTCTAG+TGG	0.587384	1.2:+5686550	MS.gene052151:CDS
AATCATCATCATCAAGGAGG+AGG	0.600518	1.2:+5686526	MS.gene052151:CDS
ACTCACCAGGGAAGATCCGA+AGG	0.608503	1.2:-5687367	None:intergenic
ATCATCATCAAGGAGGAGGA+GGG	0.622603	1.2:+5686530	MS.gene052151:CDS
ATTAACAAGGCCTATACAAG+AGG	0.622954	1.2:+5687289	MS.gene052151:CDS
AAACGAGCCACCACTTCCGG+TGG	0.627824	1.2:-5686938	None:intergenic
CATGCTTGTGAAGTATAGAG+AGG	0.648293	1.2:+5687265	MS.gene052151:CDS
CTCACCAGGGAAGATCCGAA+GGG	0.678902	1.2:-5687366	None:intergenic
CTAGGCAAGAGTTTGAAGCA+AGG	0.689197	1.2:+5686969	MS.gene052151:CDS
CATAATCATCATCATCAAGG+AGG	0.690149	1.2:+5686523	MS.gene052151:CDS
AGTATAGAGAGGAATTAACA+AGG	0.714149	1.2:+5687276	MS.gene052151:CDS
TCACCAGGGAAGATCCGAAG+GGG	0.724320	1.2:-5687365	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATATATATAAAAAAA+TGG	-	chr1.2:5686867-5686886	None:intergenic	0.0%
!!	AAATATATATATAAAAAAAT+GGG	-	chr1.2:5686866-5686885	None:intergenic	0.0%
!!!	ATTTAACTTAATAATTATCA+TGG	-	chr1.2:5687129-5687148	None:intergenic	10.0%
!!!	TTTTTAAATTGTTATCATGA+TGG	-	chr1.2:5686837-5686856	None:intergenic	15.0%
!!	TAAACAAACTATATGTAGAT+TGG	+	chr1.2:5686911-5686930	MS.gene052151:intron	20.0%
!!	TTTCAGTCAGAAATTAAATT+TGG	+	chr1.2:5686804-5686823	MS.gene052151:intron	20.0%
!!!	ATTAGAACTTATAGATTTTG+TGG	+	chr1.2:5687194-5687213	MS.gene052151:intron	20.0%
!	AAATGGTGTTTATGTTTAAC+AGG	+	chr1.2:5687229-5687248	MS.gene052151:intron	25.0%
!	TAAATTGTTATCATGATGGT+GGG	-	chr1.2:5686833-5686852	None:intergenic	25.0%
!	TTAAATTGTTATCATGATGG+TGG	-	chr1.2:5686834-5686853	None:intergenic	25.0%
!	TTAATCTCAAATTTAGTGGA+AGG	+	chr1.2:5686747-5686766	MS.gene052151:intron	25.0%
!	TTGGTTAATCTCAAATTTAG+TGG	+	chr1.2:5686743-5686762	MS.gene052151:intron	25.0%
	AGTATAGAGAGGAATTAACA+AGG	+	chr1.2:5687276-5687295	MS.gene052151:CDS	30.0%
	ATGCATAATCATCATCATCA+AGG	+	chr1.2:5686520-5686539	MS.gene052151:CDS	30.0%
!	TAGTCTTTTACAAGCTTACA+TGG	+	chr1.2:5686621-5686640	MS.gene052151:CDS	30.0%
!!	TGTAAAAGACTAGAGTATTG+AGG	-	chr1.2:5686613-5686632	None:intergenic	30.0%
!!!	AGGGGGTATATTTTGTTTTA+TGG	+	chr1.2:5687057-5687076	MS.gene052151:intron	30.0%
	AAATCCATAGCCTCTTGTAT+AGG	-	chr1.2:5687302-5687321	None:intergenic	35.0%
	ATTAACAAGGCCTATACAAG+AGG	+	chr1.2:5687289-5687308	MS.gene052151:CDS	35.0%
	CAGCTAAATACACTTTGCAA+TGG	+	chr1.2:5687341-5687360	MS.gene052151:CDS	35.0%
	CATAATCATCATCATCAAGG+AGG	+	chr1.2:5686523-5686542	MS.gene052151:CDS	35.0%
	TAGACCAATTCATGGTACAA+GGG	+	chr1.2:5687038-5687057	MS.gene052151:intron	35.0%
!	AGCTTACATGGATTGTCAAA+AGG	+	chr1.2:5686633-5686652	MS.gene052151:CDS	35.0%
!	TTGAGGATGAGCAATGATTT+TGG	-	chr1.2:5686596-5686615	None:intergenic	35.0%
!!!	TGTGGTTTTAGACGTTGAAA+TGG	+	chr1.2:5687212-5687231	MS.gene052151:intron	35.0%
	AATCATCATCATCAAGGAGG+AGG	+	chr1.2:5686526-5686545	MS.gene052151:CDS	40.0%
	AGACCAATTCATGGTACAAG+GGG	+	chr1.2:5687039-5687058	MS.gene052151:intron	40.0%
	CATGCTTGTGAAGTATAGAG+AGG	+	chr1.2:5687265-5687284	MS.gene052151:CDS	40.0%
	CTAGACCAATTCATGGTACA+AGG	+	chr1.2:5687037-5687056	MS.gene052151:intron	40.0%
	TCAAGAGAAAAAGGAGGGTT+TGG	+	chr1.2:5686724-5686743	MS.gene052151:intron	40.0%
	TCCAGAACTAGACCAATTCA+TGG	+	chr1.2:5687030-5687049	MS.gene052151:CDS	40.0%
!	AACCCTCCTTTTTCTCTTGA+AGG	-	chr1.2:5686724-5686743	None:intergenic	40.0%
!	ACCATGAATTGGTCTAGTTC+TGG	-	chr1.2:5687034-5687053	None:intergenic	40.0%
!	ACCCTCCTTTTTCTCTTGAA+GGG	-	chr1.2:5686723-5686742	None:intergenic	40.0%
!	TTCGATTTGCACTGTGATGT+CGG	+	chr1.2:5687153-5687172	MS.gene052151:intron	40.0%
	AAGGCCTATACAAGAGGCTA+TGG	+	chr1.2:5687295-5687314	MS.gene052151:CDS	45.0%
	ATCATCATCAAGGAGGAGGA+GGG	+	chr1.2:5686530-5686549	MS.gene052151:CDS	45.0%
	GACCAATTCATGGTACAAGG+GGG	+	chr1.2:5687040-5687059	MS.gene052151:intron	45.0%
	GCCCTTCAAGAGAAAAAGGA+GGG	+	chr1.2:5686719-5686738	MS.gene052151:intron	45.0%
	TACCCCCTTGTACCATGAAT+TGG	-	chr1.2:5687045-5687064	None:intergenic	45.0%
	TATGTAGATTGGAGCTCCAC+CGG	+	chr1.2:5686922-5686941	MS.gene052151:intron	45.0%
	TGCCCTTCAAGAGAAAAAGG+AGG	+	chr1.2:5686718-5686737	MS.gene052151:intron	45.0%
!	CCTTTTTCTCTTGAAGGGCA+GGG	-	chr1.2:5686718-5686737	None:intergenic	45.0%
!	CTAGGCAAGAGTTTGAAGCA+AGG	+	chr1.2:5686969-5686988	MS.gene052151:CDS	45.0%
!	TCCTTTTTCTCTTGAAGGGC+AGG	-	chr1.2:5686719-5686738	None:intergenic	45.0%
	CACTTTGCAATGGACCCCTT+CGG	+	chr1.2:5687351-5687370	MS.gene052151:CDS	50.0%
	CATCATCATCAAGGAGGAGG+AGG	+	chr1.2:5686529-5686548	MS.gene052151:CDS	50.0%
	CCCTGCCCTTCAAGAGAAAA+AGG	+	chr1.2:5686715-5686734	MS.gene052151:intron	50.0%
	GCTTCACCACTAGAACTGCT+TGG	-	chr1.2:5686559-5686578	None:intergenic	50.0%
	ACCAAACGAGCCACCACTTC+CGG	-	chr1.2:5686944-5686963	None:intergenic	55.0%
	TGGCTCGTTTGGTTGCGTCT+AGG	+	chr1.2:5686951-5686970	MS.gene052151:CDS	55.0%
!	GGGAGTCCAAGCAGTTCTAG+TGG	+	chr1.2:5686550-5686569	MS.gene052151:CDS	55.0%
	AAACGAGCCACCACTTCCGG+TGG	-	chr1.2:5686941-5686960	None:intergenic	60.0%
	ACCGGAAGTGGTGGCTCGTT+TGG	+	chr1.2:5686940-5686959	MS.gene052151:CDS	60.0%
	GATTGGAGCTCCACCGGAAG+TGG	+	chr1.2:5686928-5686947	MS.gene052151:intron	60.0%
	TGGAGCTCCACCGGAAGTGG+TGG	+	chr1.2:5686931-5686950	MS.gene052151:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	5686520	5687383	5686520	ID=MS.gene052151
chr1.2	mRNA	5686520	5687383	5686520	ID=MS.gene052151.t1;Parent=MS.gene052151
chr1.2	exon	5686520	5686654	5686520	ID=MS.gene052151.t1.exon1;Parent=MS.gene052151.t1
chr1.2	CDS	5686520	5686654	5686520	ID=cds.MS.gene052151.t1;Parent=MS.gene052151.t1
chr1.2	exon	5686929	5687051	5686929	ID=MS.gene052151.t1.exon2;Parent=MS.gene052151.t1
chr1.2	CDS	5686929	5687051	5686929	ID=cds.MS.gene052151.t1;Parent=MS.gene052151.t1
chr1.2	exon	5687251	5687383	5687251	ID=MS.gene052151.t1.exon3;Parent=MS.gene052151.t1
chr1.2	CDS	5687251	5687383	5687251	ID=cds.MS.gene052151.t1;Parent=MS.gene052151.t1

Gene Sequence

>MS.gene052151

ATGCATAATCATCATCATCAAGGAGGAGGAGGGAGTCCAAGCAGTTCTAGTGGTGAAGCTGAAGCTATAAAAGCCAAAATCATTGCTCATCCTCAATACTCTAGTCTTTTACAAGCTTACATGGATTGTCAAAAGGTCACTAGTTAATTATTATTTTTATTCATTTTCATCATTATAACAATTTATTTTTCTTAACCCTGCCCTTCAAGAGAAAAAGGAGGGTTTGGTTAATCTCAAATTTAGTGGAAGGTAAGTAAAATAGTATAATCAATAATTAATGATTGTTTCAGTCAGAAATTAAATTTGGACTCCCACCATCATGATAACAATTTAAAAAAGCATACCCATTTTTTTATATATATATTTTAATTAATTGCTATATCTCTTTAATTAAACAAACTATATGTAGATTGGAGCTCCACCGGAAGTGGTGGCTCGTTTGGTTGCGTCTAGGCAAGAGTTTGAAGCAAGGCAAAGATCTTCAGTTAACTCAAGAGAAGCTTCTAAAGATCCAGAACTAGACCAATTCATGGTACAAGGGGGTATATTTTGTTTTATGGTTTATGTTTTTTTAAAAAAATAAAAATAATTTTCGTTTAGTTTTTACCATGATAATTATTAAGTTAAATTATGTTCGATTTGCACTGTGATGTCGGCAAAAATCTTTATTATTTATTAGAACTTATAGATTTTGTGGTTTTAGACGTTGAAATGGTGTTTATGTTTAACAGGAAGCATATTATGACATGCTTGTGAAGTATAGAGAGGAATTAACAAGGCCTATACAAGAGGCTATGGATTTCATGAGAAGAATAGAAACTCAGCTAAATACACTTTGCAATGGACCCCTTCGGATCTTCCCTG

Protein sequence

>MS.gene052151.t1

MHNHHHQGGGGSPSSSSGEAEAIKAKIIAHPQYSSLLQAYMDCQKIGAPPEVVARLVASRQEFEARQRSSVNSREASKDPELDQFMEAYYDMLVKYREELTRPIQEAMDFMRRIETQLNTLCNGPLRIFP