Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31622.t1 | XP_003588536.2 | 95 | 139 | 7 | 0 | 19 | 157 | 147 | 285 | 2.10E-65 | 258.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31622.t1 | Q84MB5 | 79.1 | 139 | 29 | 0 | 19 | 157 | 136 | 274 | 2.0e-56 | 219.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31622.t1 | G7I4B9 | 95.0 | 139 | 7 | 0 | 19 | 157 | 147 | 285 | 1.5e-65 | 258.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene31622 | 0.824956 | 6.09E-54 | -1.69E-46 |
| MS.gene052176 | MS.gene31622 | 0.81637 | 5.73E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene31622 | 0.804607 | 1.99E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene31622 | 0.805195 | 1.50E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene31622 | 0.818743 | 1.67E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene31622 | -0.803879 | 2.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31622.t1 | MTR_1g008300 | 97.037 | 135 | 4 | 0 | 23 | 157 | 151 | 285 | 1.15e-80 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31622.t1 | AT5G25640 | 60.741 | 135 | 44 | 1 | 26 | 151 | 150 | 284 | 5.88e-38 | 131 |
Find 40 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATATTGCACACCTTTC+TGG | 0.256823 | 1.1:-11157708 | MS.gene31622:CDS |
| GATCCTTGAGGTGCTTATAT+TGG | 0.267418 | 1.1:-11158463 | MS.gene31622:CDS |
| TTGGAGCAAACCTTGTTTCC+TGG | 0.280672 | 1.1:-11158678 | MS.gene31622:CDS |
| TGGCTTTCTTACATTCTTAC+CGG | 0.307877 | 1.1:-11158703 | MS.gene31622:CDS |
| CCAACTGAAACAGTGTTTCT+TGG | 0.313482 | 1.1:+11158646 | None:intergenic |
| ACTGTTTCAGTTGGAGCTTC+TGG | 0.317045 | 1.1:-11158637 | MS.gene31622:CDS |
| CACCTTTCTGGTGCTCTTGT+TGG | 0.320132 | 1.1:-11157696 | MS.gene31622:CDS |
| ATCCTTGAGGTGCTTATATT+GGG | 0.342592 | 1.1:-11158462 | MS.gene31622:CDS |
| TGCTCTTGTTGGTGTGCTCT+TGG | 0.361990 | 1.1:-11157685 | MS.gene31622:CDS |
| AAAATGTTGCTGTGAGAAAC+TGG | 0.380861 | 1.1:+11159096 | None:intergenic |
| CACCAGAAAGGTGTGCAATA+TGG | 0.382272 | 1.1:+11157706 | None:intergenic |
| TTGTTGGTGTGCTCTTGGTT+TGG | 0.405808 | 1.1:-11157680 | MS.gene31622:CDS |
| TATTTCACAGCAGGCCTGAT+TGG | 0.416384 | 1.1:-11159121 | MS.gene31622:CDS |
| TGATTTAGATGTCATGGGAT+TGG | 0.421546 | 1.1:-11158491 | MS.gene31622:intron |
| TAGCCTCCACGAAAGGTTCC+TGG | 0.429863 | 1.1:+11157747 | None:intergenic |
| CAATTTCATGTACCTTTACA+AGG | 0.431794 | 1.1:+11158584 | None:intergenic |
| TCTTACATTCTTACCGGTGT+TGG | 0.432285 | 1.1:-11158697 | MS.gene31622:CDS |
| GTGAGAAACTGGAACCAATC+AGG | 0.449299 | 1.1:+11159107 | None:intergenic |
| CATTGTATTTATTTCACAGC+AGG | 0.455684 | 1.1:-11159130 | MS.gene31622:CDS |
| AGGAAAGCTTGTTGAAGAAG+AGG | 0.467402 | 1.1:-11158743 | MS.gene31622:CDS |
| TCTTTACCAGGAACCTTTCG+TGG | 0.471320 | 1.1:-11157753 | MS.gene31622:CDS |
| TACTATCAGCGTCCTTGTAA+AGG | 0.478499 | 1.1:-11158596 | MS.gene31622:intron |
| AACAAGGTTTGCTCCAACAC+CGG | 0.484733 | 1.1:+11158684 | None:intergenic |
| CCAAGAAACACTGTTTCAGT+TGG | 0.488646 | 1.1:-11158646 | MS.gene31622:CDS |
| GGGTCAGTTTGTTGTAGAGA+AGG | 0.498677 | 1.1:-11158442 | MS.gene31622:intron |
| GAACTTGATTTAGATGTCAT+GGG | 0.502041 | 1.1:-11158496 | MS.gene31622:intron |
| TGAACTTGATTTAGATGTCA+TGG | 0.507252 | 1.1:-11158497 | MS.gene31622:intron |
| TTTAGATGTCATGGGATTGG+AGG | 0.517050 | 1.1:-11158488 | MS.gene31622:intron |
| CAAGCTTCAACATCTTTACC+AGG | 0.545205 | 1.1:-11157765 | MS.gene31622:CDS |
| GTTTCTTGGTAAAATCAGCC+AGG | 0.562273 | 1.1:+11158660 | None:intergenic |
| GTAAAATCAGCCAGGAAACA+AGG | 0.565010 | 1.1:+11158668 | None:intergenic |
| CACCAACAAGAGCACCAGAA+AGG | 0.589348 | 1.1:+11157694 | None:intergenic |
| GGAAAGCTTGTTGAAGAAGA+GGG | 0.589617 | 1.1:-11158742 | MS.gene31622:CDS |
| TCTGTAGGTCGATGGCATCA+AGG | 0.589873 | 1.1:-11159153 | None:intergenic |
| AAGCTTGTTGAAGAAGAGGG+AGG | 0.592709 | 1.1:-11158739 | MS.gene31622:CDS |
| CAATGCGTAGCCTCCACGAA+AGG | 0.605361 | 1.1:+11157740 | None:intergenic |
| TATCGGTCTGAAGAATCAGA+AGG | 0.633251 | 1.1:+11157642 | None:intergenic |
| TTACCAGGAACCTTTCGTGG+AGG | 0.643485 | 1.1:-11157750 | MS.gene31622:CDS |
| GACCCAATATAAGCACCTCA+AGG | 0.644183 | 1.1:+11158460 | None:intergenic |
| GGATTGGAGGAAGATCCTTG+AGG | 0.747907 | 1.1:-11158475 | MS.gene31622:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATTCTTAAAAAAAAAAC+AGG | + | chr1.1:11157777-11157796 | None:intergenic | 15.0% |
| !! | AAAGATACATATTGAAATTC+TGG | - | chr1.1:11158492-11158511 | MS.gene31622:intron | 20.0% |
| !! | ATAACAAACTACAAAATAGT+TGG | - | chr1.1:11158642-11158661 | MS.gene31622:CDS | 20.0% |
| !! | CAAATATGTATAAGAAGAAA+AGG | + | chr1.1:11157929-11157948 | None:intergenic | 20.0% |
| !! | GAATTTCAATATGTATCTTT+AGG | + | chr1.1:11158492-11158511 | None:intergenic | 20.0% |
| !! | GGAAAAAAGAAAGAAAAAAA+AGG | + | chr1.1:11158380-11158399 | None:intergenic | 20.0% |
| !!! | CTTTTCTTCTTATACATATT+TGG | - | chr1.1:11157927-11157946 | MS.gene31622:intron | 20.0% |
| !!! | TTTATCCATTTTCTATTTCT+GGG | - | chr1.1:11157824-11157843 | MS.gene31622:intron | 20.0% |
| !!! | TTTTATCCATTTTCTATTTC+TGG | - | chr1.1:11157823-11157842 | MS.gene31622:intron | 20.0% |
| ! | CTTCTTATACATATTTGGTA+AGG | - | chr1.1:11157932-11157951 | MS.gene31622:intron | 25.0% |
| !! | AGATAAAAAAGCTCATGTTT+TGG | - | chr1.1:11158897-11158916 | MS.gene31622:intron | 25.0% |
| !! | TTAGAGTATTAGTCAGATTA+TGG | + | chr1.1:11157879-11157898 | None:intergenic | 25.0% |
| !!! | ATCATTTAGAGTTGTTTTAC+AGG | + | chr1.1:11158410-11158429 | None:intergenic | 25.0% |
| !!! | TCATTTAGAGTTGTTTTACA+GGG | + | chr1.1:11158409-11158428 | None:intergenic | 25.0% |
| !!! | TTTTAAGAATTTGCATTCCA+CGG | - | chr1.1:11157784-11157803 | MS.gene31622:intron | 25.0% |
| AATATATTGCAAGCCATTTG+CGG | + | chr1.1:11158826-11158845 | None:intergenic | 30.0% | |
| ACAAACCCAGAAATAGAAAA+TGG | + | chr1.1:11157832-11157851 | None:intergenic | 30.0% | |
| ACATATTTGACACATCTGAA+GGG | + | chr1.1:11158740-11158759 | None:intergenic | 30.0% | |
| CAATTTCATGTACCTTTACA+AGG | + | chr1.1:11158202-11158221 | None:intergenic | 30.0% | |
| CATTGTATTTATTTCACAGC+AGG | - | chr1.1:11157653-11157672 | MS.gene31622:CDS | 30.0% | |
| GAACTTGATTTAGATGTCAT+GGG | - | chr1.1:11158287-11158306 | MS.gene31622:intron | 30.0% | |
| TACATATTTGACACATCTGA+AGG | + | chr1.1:11158741-11158760 | None:intergenic | 30.0% | |
| TGAACTTGATTTAGATGTCA+TGG | - | chr1.1:11158286-11158305 | MS.gene31622:intron | 30.0% | |
| TTTAAGAATTTGCATTCCAC+GGG | - | chr1.1:11157785-11157804 | MS.gene31622:intron | 30.0% | |
| ! | AACCAAAAAGTGTAAGAACT+TGG | + | chr1.1:11158267-11158286 | None:intergenic | 30.0% |
| ! | ACTAATACTCTAACTTGTAC+AGG | - | chr1.1:11157886-11157905 | MS.gene31622:intron | 30.0% |
| ! | CAACATTTTGTCATGCTAAT+TGG | - | chr1.1:11157701-11157720 | MS.gene31622:CDS | 30.0% |
| ! | CTGTTATTTGTGAGTGTAAT+AGG | - | chr1.1:11158981-11159000 | MS.gene31622:intron | 30.0% |
| !!! | ATGTTTTGGTCTTGTTATTC+GGG | - | chr1.1:11158911-11158930 | MS.gene31622:intron | 30.0% |
| !!! | CATGTTTTGGTCTTGTTATT+CGG | - | chr1.1:11158910-11158929 | MS.gene31622:intron | 30.0% |
| AAAATGTTGCTGTGAGAAAC+TGG | + | chr1.1:11157690-11157709 | None:intergenic | 35.0% | |
| AAGAACTTGGAGAAACAAAC+TGG | + | chr1.1:11158254-11158273 | None:intergenic | 35.0% | |
| AGCACACTCATGAAATTTAC+AGG | + | chr1.1:11158519-11158538 | None:intergenic | 35.0% | |
| GGGTTTATACTTGAACAACT+AGG | - | chr1.1:11158931-11158950 | MS.gene31622:intron | 35.0% | |
| TGATTTAGATGTCATGGGAT+TGG | - | chr1.1:11158292-11158311 | MS.gene31622:intron | 35.0% | |
| TGGCTTTCTTACATTCTTAC+CGG | - | chr1.1:11158080-11158099 | MS.gene31622:intron | 35.0% | |
| TTAGGAGATGCAAATCTGAA+AGG | + | chr1.1:11158474-11158493 | None:intergenic | 35.0% | |
| TTTGTGAGTGTAATAGGTCA+TGG | - | chr1.1:11158987-11159006 | MS.gene31622:intron | 35.0% | |
| ! | ATCCTTGAGGTGCTTATATT+GGG | - | chr1.1:11158321-11158340 | MS.gene31622:intron | 35.0% |
| ! | CTCCAAGTTCTTACACTTTT+TGG | - | chr1.1:11158262-11158281 | MS.gene31622:intron | 35.0% |
| ! | TATTTCGTTGCTTTCACAAC+AGG | - | chr1.1:11158020-11158039 | MS.gene31622:intron | 35.0% |
| !! | AGTTGTTTTACAGGGCAAAA+CGG | + | chr1.1:11158401-11158420 | None:intergenic | 35.0% |
| AACCATATTGCACACCTTTC+TGG | - | chr1.1:11159075-11159094 | MS.gene31622:intron | 40.0% | |
| CAAGCTTCAACATCTTTACC+AGG | - | chr1.1:11159018-11159037 | MS.gene31622:intron | 40.0% | |
| CCAACTGAAACAGTGTTTCT+TGG | + | chr1.1:11158140-11158159 | None:intergenic | 40.0% | |
| CCAAGAAACACTGTTTCAGT+TGG | - | chr1.1:11158137-11158156 | MS.gene31622:intron | 40.0% | |
| GTAAAATCAGCCAGGAAACA+AGG | + | chr1.1:11158118-11158137 | None:intergenic | 40.0% | |
| GTTTCTTGGTAAAATCAGCC+AGG | + | chr1.1:11158126-11158145 | None:intergenic | 40.0% | |
| TACTATCAGCGTCCTTGTAA+AGG | - | chr1.1:11158187-11158206 | MS.gene31622:intron | 40.0% | |
| TATCGGTCTGAAGAATCAGA+AGG | + | chr1.1:11159144-11159163 | None:intergenic | 40.0% | |
| TATTTGGTAAGGCTGTGACA+CGG | - | chr1.1:11157943-11157962 | MS.gene31622:intron | 40.0% | |
| TCTTACATTCTTACCGGTGT+TGG | - | chr1.1:11158086-11158105 | MS.gene31622:intron | 40.0% | |
| TTTAGATGTCATGGGATTGG+AGG | - | chr1.1:11158295-11158314 | MS.gene31622:intron | 40.0% | |
| ! | CATCTGCGTGTATTTTATCG+CGG | + | chr1.1:11158621-11158640 | None:intergenic | 40.0% |
| ! | GATCCTTGAGGTGCTTATAT+TGG | - | chr1.1:11158320-11158339 | MS.gene31622:intron | 40.0% |
| !! | AGGAAAGCTTGTTGAAGAAG+AGG | - | chr1.1:11158040-11158059 | MS.gene31622:intron | 40.0% |
| !! | GGAAAGCTTGTTGAAGAAGA+GGG | - | chr1.1:11158041-11158060 | MS.gene31622:intron | 40.0% |
| AATTTGCATTCCACGGGAAC+AGG | - | chr1.1:11157791-11157810 | MS.gene31622:intron | 45.0% | |
| ACACGGTCACTCTTCTTTCT+TGG | - | chr1.1:11157960-11157979 | MS.gene31622:intron | 45.0% | |
| ACTGTTTCAGTTGGAGCTTC+TGG | - | chr1.1:11158146-11158165 | MS.gene31622:intron | 45.0% | |
| GACCCAATATAAGCACCTCA+AGG | + | chr1.1:11158326-11158345 | None:intergenic | 45.0% | |
| GTGAGAAACTGGAACCAATC+AGG | + | chr1.1:11157679-11157698 | None:intergenic | 45.0% | |
| TATTTCACAGCAGGCCTGAT+TGG | - | chr1.1:11157662-11157681 | MS.gene31622:CDS | 45.0% | |
| TCTTTACCAGGAACCTTTCG+TGG | - | chr1.1:11159030-11159049 | MS.gene31622:intron | 45.0% | |
| TTGTTGGTGTGCTCTTGGTT+TGG | - | chr1.1:11159103-11159122 | MS.gene31622:CDS | 45.0% | |
| ! | AACAAGGTTTGCTCCAACAC+CGG | + | chr1.1:11158102-11158121 | None:intergenic | 45.0% |
| ! | AGGGAGGAAGTTTTGCTTTG+TGG | - | chr1.1:11158060-11158079 | MS.gene31622:intron | 45.0% |
| ! | CACCAGAAAGGTGTGCAATA+TGG | + | chr1.1:11159080-11159099 | None:intergenic | 45.0% |
| ! | TTGGAGCAAACCTTGTTTCC+TGG | - | chr1.1:11158105-11158124 | MS.gene31622:intron | 45.0% |
| !! | AAGCTTGTTGAAGAAGAGGG+AGG | - | chr1.1:11158044-11158063 | MS.gene31622:intron | 45.0% |
| !! | ATCTGAAGGGAGCACTTTAG+AGG | + | chr1.1:11158727-11158746 | None:intergenic | 45.0% |
| !! | GGGTCAGTTTGTTGTAGAGA+AGG | - | chr1.1:11158341-11158360 | MS.gene31622:intron | 45.0% |
| !!! | GAGCTTCTGGTGCTGTTTTT+GGG | - | chr1.1:11158159-11158178 | MS.gene31622:intron | 45.0% |
| GGATTGGAGGAAGATCCTTG+AGG | - | chr1.1:11158308-11158327 | MS.gene31622:intron | 50.0% | |
| TGCTCTTGTTGGTGTGCTCT+TGG | - | chr1.1:11159098-11159117 | MS.gene31622:CDS | 50.0% | |
| TGCTTGACTCATGCCGCAAA+TGG | - | chr1.1:11158810-11158829 | MS.gene31622:intron | 50.0% | |
| TTACCAGGAACCTTTCGTGG+AGG | - | chr1.1:11159033-11159052 | MS.gene31622:intron | 50.0% | |
| ! | CACCTTTCTGGTGCTCTTGT+TGG | - | chr1.1:11159087-11159106 | MS.gene31622:CDS | 50.0% |
| !! | CACCAACAAGAGCACCAGAA+AGG | + | chr1.1:11159092-11159111 | None:intergenic | 50.0% |
| !!! | GGAGCTTCTGGTGCTGTTTT+TGG | - | chr1.1:11158158-11158177 | MS.gene31622:intron | 50.0% |
| AGCATGTGATCCTGTTCCCG+TGG | + | chr1.1:11157804-11157823 | None:intergenic | 55.0% | |
| CAATGCGTAGCCTCCACGAA+AGG | + | chr1.1:11159046-11159065 | None:intergenic | 55.0% | |
| TAGCCTCCACGAAAGGTTCC+TGG | + | chr1.1:11159039-11159058 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 11157641 | 11159164 | 11157641 | ID=MS.gene31622 |
| chr1.1 | mRNA | 11157641 | 11159164 | 11157641 | ID=MS.gene31622.t1;Parent=MS.gene31622 |
| chr1.1 | exon | 11159083 | 11159164 | 11159083 | ID=MS.gene31622.t1.exon1;Parent=MS.gene31622.t1 |
| chr1.1 | CDS | 11159083 | 11159164 | 11159083 | ID=cds.MS.gene31622.t1;Parent=MS.gene31622.t1 |
| chr1.1 | exon | 11158597 | 11158763 | 11158597 | ID=MS.gene31622.t1.exon2;Parent=MS.gene31622.t1 |
| chr1.1 | CDS | 11158597 | 11158763 | 11158597 | ID=cds.MS.gene31622.t1;Parent=MS.gene31622.t1 |
| chr1.1 | exon | 11158443 | 11158505 | 11158443 | ID=MS.gene31622.t1.exon3;Parent=MS.gene31622.t1 |
| chr1.1 | CDS | 11158443 | 11158505 | 11158443 | ID=cds.MS.gene31622.t1;Parent=MS.gene31622.t1 |
| chr1.1 | exon | 11157641 | 11157802 | 11157641 | ID=MS.gene31622.t1.exon4;Parent=MS.gene31622.t1 |
| chr1.1 | CDS | 11157641 | 11157802 | 11157641 | ID=cds.MS.gene31622.t1;Parent=MS.gene31622.t1 |
| Gene Sequence |
| Protein sequence |
