AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32079

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32079.t1 MTR_4g102520 97.603 584 14 0 1 584 1 584 0.0 1200
MS.gene32079.t1 MTR_1g072740 98.505 535 7 1 1 535 1 534 0.0 1095
MS.gene32079.t1 MTR_8g467040 33.226 310 193 4 282 581 107 412 1.30e-51 183
MS.gene32079.t1 MTR_4g119620 27.964 329 210 10 270 584 6 321 2.39e-33 130
MS.gene32079.t1 MTR_1g072140 25.325 308 217 8 281 584 14 312 1.36e-29 119
MS.gene32079.t1 MTR_7g086360 26.730 318 220 9 268 581 32 340 2.62e-26 110
MS.gene32079.t1 MTR_8g092620 27.687 307 203 10 282 584 6 297 1.21e-24 104
MS.gene32079.t1 MTR_8g092620 24.514 257 147 8 283 496 47 299 1.72e-12 68.9
MS.gene32079.t1 MTR_4g104380 25.962 312 206 8 282 584 218 513 2.18e-24 107
MS.gene32079.t1 MTR_4g104380 33.019 106 65 3 273 374 289 392 6.80e-12 68.6
MS.gene32079.t1 MTR_3g075400 26.471 306 214 8 281 584 212 508 3.10e-23 103
MS.gene32079.t1 MTR_8g092620 27.541 305 202 10 282 582 13 302 4.41e-23 100
MS.gene32079.t1 MTR_2g436510 26.299 308 195 12 291 584 167 456 2.92e-19 91.3
MS.gene32079.t1 MTR_7g076230 26.132 287 188 9 292 560 367 647 5.60e-19 91.7
MS.gene32079.t1 MTR_8g092620 28.037 214 139 8 282 491 6 208 1.52e-16 79.7
MS.gene32079.t1 MTR_6g004040 29.825 171 111 6 285 453 92 255 1.55e-16 83.6
MS.gene32079.t1 MTR_6g004040 23.264 288 197 10 290 565 12 287 1.64e-14 77.4
MS.gene32079.t1 MTR_6g004040 24.868 189 135 4 396 584 31 212 3.08e-13 73.2
MS.gene32079.t1 MTR_6g004040 31.538 130 80 4 284 410 133 256 2.20e-11 67.4
MS.gene32079.t1 MTR_1g069345 24.691 324 227 11 267 584 191 503 7.60e-16 80.9
MS.gene32079.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 1.48e-15 80.9
MS.gene32079.t1 MTR_2g012630 23.737 198 144 4 387 584 21 211 2.81e-13 73.6
MS.gene32079.t1 MTR_2g012630 22.867 293 198 10 287 565 8 286 1.26e-12 71.6
MS.gene32079.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 1.86e-15 80.5
MS.gene32079.t1 MTR_2g012630 23.737 198 144 4 387 584 21 211 4.18e-13 73.2
MS.gene32079.t1 MTR_2g012630 22.867 293 198 10 287 565 8 286 1.66e-12 71.2
MS.gene32079.t1 MTR_1g054300 23.622 381 201 11 285 584 110 481 2.08e-15 79.3
MS.gene32079.t1 MTR_1g097140 25.329 304 204 14 287 584 73 359 3.48e-15 78.2
MS.gene32079.t1 MTR_3g052220 23.824 319 217 11 283 584 202 511 2.63e-14 76.3
MS.gene32079.t1 MTR_5g090420 21.338 314 234 8 276 584 243 548 2.68e-14 76.3
MS.gene32079.t1 MTR_3g116500 24.127 315 215 10 289 584 60 369 2.29e-13 72.4
MS.gene32079.t1 MTR_3g116500 26.222 225 150 7 373 584 38 259 9.43e-13 69.3
MS.gene32079.t1 MTR_8g106790 23.022 278 183 12 282 539 54 320 1.24e-12 69.7
MS.gene32079.t1 MTR_2g060520 21.429 322 228 8 283 584 115 431 6.74e-12 68.2
MS.gene32079.t1 MTR_4g123597 22.812 320 212 14 285 584 347 651 7.19e-12 68.9
MS.gene32079.t1 MTR_4g105700 21.639 305 212 11 298 584 316 611 9.32e-12 68.6
MS.gene32079.t1 MTR_1g034785 24.339 189 117 5 285 451 109 293 5.39e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32079.t1 AT1G10580 77.607 585 117 4 1 584 1 572 0.0 937
MS.gene32079.t1 AT5G54520 32.915 319 204 3 274 584 140 456 8.99e-55 192
MS.gene32079.t1 AT3G49660 25.321 312 215 9 281 584 11 312 2.17e-29 119
MS.gene32079.t1 AT3G49660 28.767 219 145 6 286 499 105 317 1.98e-25 107
MS.gene32079.t1 AT5G64730 27.508 309 201 11 282 584 6 297 8.24e-27 111
MS.gene32079.t1 AT4G15900 29.967 307 183 12 292 584 174 462 3.56e-26 112
MS.gene32079.t1 AT2G43770 26.460 291 206 7 291 578 50 335 1.16e-25 108
MS.gene32079.t1 AT4G02730 24.759 311 216 10 281 584 30 329 1.51e-24 105
MS.gene32079.t1 AT3G16650 28.990 307 185 13 292 584 168 455 4.80e-22 100
MS.gene32079.t1 AT5G50230 24.407 295 199 7 282 567 213 492 5.96e-20 94.0
MS.gene32079.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.48e-17 87.0
MS.gene32079.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.34e-14 77.8
MS.gene32079.t1 AT5G23430 25.000 192 131 4 396 584 31 212 4.31e-13 72.8
MS.gene32079.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.21e-11 67.0
MS.gene32079.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.51e-17 86.7
MS.gene32079.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.51e-17 86.7
MS.gene32079.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.51e-17 86.7
MS.gene32079.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.53e-17 87.0
MS.gene32079.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.35e-14 77.8
MS.gene32079.t1 AT5G23430 25.000 192 131 4 396 584 31 212 4.38e-13 72.8
MS.gene32079.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.18e-11 67.0
MS.gene32079.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.53e-17 87.0
MS.gene32079.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.35e-14 77.8
MS.gene32079.t1 AT5G23430 25.000 192 131 4 396 584 31 212 4.38e-13 72.8
MS.gene32079.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.18e-11 67.0
MS.gene32079.t1 AT5G08390 23.913 276 192 9 287 561 9 267 3.40e-17 85.9
MS.gene32079.t1 AT5G08390 32.000 150 94 5 285 432 92 235 2.04e-15 80.5
MS.gene32079.t1 AT5G08390 25.000 192 131 4 396 584 31 212 3.33e-13 73.2
MS.gene32079.t1 AT5G08390 32.283 127 83 3 284 410 133 256 1.91e-12 70.9
MS.gene32079.t1 AT1G61210 31.429 175 112 6 285 458 91 258 7.41e-17 85.1
MS.gene32079.t1 AT1G61210 25.714 280 182 10 292 567 13 270 7.83e-16 81.6
MS.gene32079.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.28e-11 68.2
MS.gene32079.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.39e-11 68.2
MS.gene32079.t1 AT1G61210 31.429 175 112 6 285 458 91 258 1.03e-16 84.7
MS.gene32079.t1 AT1G61210 25.714 280 182 10 292 567 13 270 1.12e-15 81.3
MS.gene32079.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.50e-11 68.2
MS.gene32079.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.63e-11 68.2
MS.gene32079.t1 AT1G61210 31.429 175 112 6 285 458 91 258 1.08e-16 84.7
MS.gene32079.t1 AT1G61210 25.714 280 182 10 292 567 13 270 1.31e-15 81.3
MS.gene32079.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.47e-11 68.2
MS.gene32079.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.66e-11 68.2
MS.gene32079.t1 AT1G15440 26.457 223 156 5 292 510 347 565 1.54e-16 84.0
MS.gene32079.t1 AT1G15440 26.457 223 156 5 292 510 387 605 1.93e-16 83.6
MS.gene32079.t1 AT5G43920 24.688 320 218 12 278 584 204 513 3.06e-16 82.4
MS.gene32079.t1 AT1G11160 24.742 291 195 9 287 565 8 286 5.45e-16 82.4
MS.gene32079.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.26e-15 79.0
MS.gene32079.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.18e-14 78.2
MS.gene32079.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.48e-11 66.2
MS.gene32079.t1 AT1G11160 24.742 291 195 9 287 565 8 286 5.79e-16 82.0
MS.gene32079.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.65e-15 79.0
MS.gene32079.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.24e-14 77.8
MS.gene32079.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.71e-11 66.2
MS.gene32079.t1 AT1G11160 24.742 291 195 9 287 565 8 286 6.10e-16 82.0
MS.gene32079.t1 AT1G11160 27.684 177 120 6 285 460 91 260 7.07e-15 78.6
MS.gene32079.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.11e-14 78.2
MS.gene32079.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.82e-11 66.2
MS.gene32079.t1 AT1G11160 27.684 177 120 6 285 460 49 218 5.99e-15 79.0
MS.gene32079.t1 AT1G11160 25.749 167 117 4 418 584 10 169 1.38e-12 71.2
MS.gene32079.t1 AT1G11160 24.803 254 168 8 324 565 2 244 1.28e-11 68.2
MS.gene32079.t1 AT1G11160 30.534 131 80 6 284 410 90 213 5.44e-11 66.2
MS.gene32079.t1 AT3G18130 24.907 269 175 11 291 539 60 321 6.71e-14 73.6
MS.gene32079.t1 AT3G18130 24.907 269 184 9 293 553 14 272 1.31e-11 66.6
MS.gene32079.t1 AT5G52820 25.000 300 200 11 291 584 191 471 2.48e-13 72.8
MS.gene32079.t1 AT1G48630 24.907 269 177 11 291 541 60 321 5.96e-13 70.9
MS.gene32079.t1 AT1G48630 24.542 273 188 9 289 553 10 272 6.65e-12 67.4
MS.gene32079.t1 AT2G33340 25.591 254 173 9 285 532 255 498 6.12e-13 72.0
MS.gene32079.t1 AT2G33340 25.591 254 173 9 285 532 255 498 6.12e-13 72.0
MS.gene32079.t1 AT5G16750 25.444 169 118 5 289 452 55 220 8.14e-13 72.0
MS.gene32079.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.20e-12 71.2
MS.gene32079.t1 AT2G41500 25.472 212 152 6 286 496 331 537 4.28e-12 69.3
MS.gene32079.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.20e-12 71.2
MS.gene32079.t1 AT2G41500 25.472 212 152 6 286 496 331 537 4.28e-12 69.3
MS.gene32079.t1 AT1G73720 24.451 319 214 12 283 584 201 509 3.42e-12 69.3
MS.gene32079.t1 AT3G21540 23.864 264 174 12 334 584 402 651 8.83e-12 68.9
MS.gene32079.t1 AT3G21540 27.094 203 134 8 303 498 26 221 6.14e-11 66.2
MS.gene32079.t1 AT1G79990 22.460 187 141 3 310 494 72 256 1.45e-11 68.2
MS.gene32079.t1 AT2G33340 27.011 174 117 6 285 453 255 423 1.51e-11 67.4
MS.gene32079.t1 AT1G79990 22.460 187 141 3 310 494 72 256 1.74e-11 67.8
MS.gene32079.t1 AT1G18080 23.420 269 182 9 291 541 60 322 1.79e-11 66.2
MS.gene32079.t1 AT1G18080 22.695 282 161 7 289 562 10 242 1.99e-11 65.9
MS.gene32079.t1 AT5G60940 23.125 320 224 9 282 584 111 425 4.51e-11 65.5
MS.gene32079.t1 AT5G60940 23.125 320 224 9 282 584 19 333 5.74e-11 64.7
MS.gene32079.t1 AT5G67320 23.464 358 202 14 281 575 252 600 6.17e-11 65.9

Find 156 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATACTAAGGTTAAAATT+TGG 0.172435 1.2:+55526724 MS.gene32079:CDS
GATGGGTTTGGAGAGGGTTT+TGG 0.192541 1.2:-55525941 None:intergenic
GTGAGGGTAAGTGTTGGTTC+TGG 0.241552 1.2:+55527381 MS.gene32079:CDS
AGCATGGTGTCGTCGACTTT+GGG 0.273615 1.2:-55525898 None:intergenic
ACATTCTGTTTATCCTCATC+TGG 0.288775 1.2:-55526945 None:intergenic
TAAATGTATGAGGACTTATA+TGG 0.290113 1.2:+55526764 MS.gene32079:CDS
TTACTCGCCTTAGCATCTTT+TGG 0.297645 1.2:-55526588 None:intergenic
GGATACTAAGGTTAAAATTT+GGG 0.302166 1.2:+55526725 MS.gene32079:CDS
CAAGAGTATGATCAGCATTT+GGG 0.309524 1.2:+55527032 MS.gene32079:CDS
CGATAAGAATATTAAGTATT+GGG 0.318797 1.2:+55526854 MS.gene32079:CDS
TTGAGTTGGGTCGATGGGTT+TGG 0.324589 1.2:-55525953 None:intergenic
TCCCTCAGGGTATGGGAATA+TGG 0.330247 1.2:+55527119 MS.gene32079:CDS
TTGGCTCGCCGCTCAGAGTT+TGG 0.331077 1.2:+55527220 MS.gene32079:CDS
GTTCAGTGGGATATGAATAC+TGG 0.343180 1.2:+55526999 MS.gene32079:CDS
CAGCTCTGGGCCCCGATTCA+AGG 0.351014 1.2:+55526006 MS.gene32079:CDS
TGAGGGTAAGTGTTGGTTCT+GGG 0.355149 1.2:+55527382 MS.gene32079:CDS
GAGCATGGTGTCGTCGACTT+TGG 0.357237 1.2:-55525899 None:intergenic
TCAAGAGTATGATCAGCATT+TGG 0.367145 1.2:+55527031 MS.gene32079:CDS
GCGGCGAGCCAATTGGCATT+GGG 0.372981 1.2:-55527209 None:intergenic
TGGGGCATTCGAAAGCTGTT+AGG 0.375620 1.2:+55526784 MS.gene32079:CDS
CGAAAGGGGTTTCTGCGATT+AGG 0.375961 1.2:+55526661 MS.gene32079:CDS
TTTGCTGGACATATTGTTGC+TGG 0.377860 1.2:+55527302 MS.gene32079:CDS
GGTGAGATGGGCCTTGAATC+GGG 0.381007 1.2:-55526017 None:intergenic
TGTCGTTGTTGTCGGTGTAT+TGG 0.386921 1.2:-55525795 None:intergenic
CGATATAACCTGCCCCGTTT+CGG 0.387388 1.2:-55526880 None:intergenic
AAAGATAAGAGTACCTTTCA+TGG 0.388138 1.2:+55526522 MS.gene32079:CDS
TTGTGCTATAAATAAGTATC+TGG 0.390504 1.2:-55527247 None:intergenic
ACCCTACCTACGACCAGCTC+TGG 0.397227 1.2:+55525992 MS.gene32079:CDS
CACTTCACCCCAATGCCAAT+TGG 0.397316 1.2:+55527201 MS.gene32079:CDS
AGAATCAAATGACCCGAATT+CGG 0.398493 1.2:-55526690 None:intergenic
GGGATTATCAAGGTAGGTCT+TGG 0.401179 1.2:+55526556 MS.gene32079:CDS
GTAAGTGTTGGTTCTGGGAT+TGG 0.407813 1.2:+55527387 MS.gene32079:CDS
CTTGAATCGGGGCCCAGAGC+TGG 0.409734 1.2:-55526005 None:intergenic
GCAAAGTAGCGACGTGTGGC+TGG 0.411956 1.2:+55527489 MS.gene32079:CDS
AGCGGCGAGCCAATTGGCAT+TGG 0.418827 1.2:-55527210 None:intergenic
GGGTGAGATGGGCCTTGAAT+CGG 0.419829 1.2:-55526018 None:intergenic
ACGATAAGAATATTAAGTAT+TGG 0.420715 1.2:+55526853 MS.gene32079:CDS
TCTTCTTCTGTTCACGGTGT+GGG 0.426132 1.2:-55526242 None:intergenic
GAACGGGAAGGGAGGAGACG+AGG 0.429345 1.2:-55525868 None:intergenic
AAGTATTGGGATACCGAAAC+GGG 0.432545 1.2:+55526867 MS.gene32079:CDS
ATGACAAGTCCCTCAGGGTA+TGG 0.434880 1.2:+55527111 MS.gene32079:CDS
CTCTTCTTCTGTTCACGGTG+TGG 0.437638 1.2:-55526243 None:intergenic
TTTGGGGTCGGCGACAGTTA+GGG 0.443652 1.2:-55525923 None:intergenic
TCAATACTGGTAAATGTATG+AGG 0.444000 1.2:+55526754 MS.gene32079:CDS
GCCCAGAGCTGGTCGTAGGT+AGG 0.447192 1.2:-55525994 None:intergenic
GGGTTAAGCTTCACAACATA+AGG 0.453310 1.2:-55526924 None:intergenic
AAATGTATGAGGACTTATAT+GGG 0.458158 1.2:+55526765 MS.gene32079:CDS
TGGGTCGATGGGTTTGGAGA+GGG 0.461924 1.2:-55525947 None:intergenic
TTCGGGTCATTTGATTCTCT+CGG 0.463515 1.2:+55526695 MS.gene32079:CDS
TGGTTGCGAGTGGCATCCTC+TGG 0.463609 1.2:+55527460 MS.gene32079:CDS
GGGCGGCGGAACGGGAAGGG+AGG 0.465040 1.2:-55525876 None:intergenic
GGCATTGGGGTGAAGTGAAA+TGG 0.465903 1.2:-55527195 None:intergenic
TGGATCGTTGTCGTTGTTGT+CGG 0.468729 1.2:-55525803 None:intergenic
TAGCGACGTGTGGCTGGGAC+GGG 0.469608 1.2:+55527495 MS.gene32079:CDS
CCTTTCCTCTTTCCCATGAA+AGG 0.470308 1.2:-55526535 None:intergenic
AGGTGTTGAGTTGGGTCGAT+GGG 0.471106 1.2:-55525958 None:intergenic
CCCAGAGCTGGTCGTAGGTA+GGG 0.477469 1.2:-55525993 None:intergenic
AACAACCACGCCTCGGAAGC+CGG 0.477605 1.2:-55526354 None:intergenic
CTGATATACTTTATAACTAC+AGG 0.479452 1.2:-55527146 None:intergenic
GAGTCCTTGGGCTGGGAAGA+AGG 0.482068 1.2:+55526389 MS.gene32079:CDS
GGGTGTTGAGATCGAGAATC+CGG 0.488285 1.2:+55526335 MS.gene32079:CDS
TGTTGCTGGATATGCTTGTC+AGG 0.489525 1.2:+55527316 MS.gene32079:CDS
CCAGTGATGACAAGTCCCTC+AGG 0.489956 1.2:+55527105 MS.gene32079:CDS
CTCGGCTGGTATGGATACTA+AGG 0.491505 1.2:+55526713 MS.gene32079:CDS
AGGCGATAAGGTGTTGAGTT+GGG 0.492099 1.2:-55525966 None:intergenic
TTTGATTCTCTCGGCTGGTA+TGG 0.492749 1.2:+55526704 MS.gene32079:CDS
GGGGCATTCGAAAGCTGTTA+GGG 0.493324 1.2:+55526785 MS.gene32079:CDS
CGAATCTCTTAAGAAGAAAG+AGG 0.496260 1.2:+55526272 MS.gene32079:CDS
CTTTCATGGGAAAGAGGAAA+GGG 0.500065 1.2:+55526536 MS.gene32079:CDS
GGTCATTTGATTCTCTCGGC+TGG 0.501830 1.2:+55526699 MS.gene32079:CDS
CATTCTGTTTATCCTCATCT+GGG 0.502022 1.2:-55526944 None:intergenic
CCCTACCTACGACCAGCTCT+GGG 0.505645 1.2:+55525993 MS.gene32079:CDS
CGAAGAGATTGGTACATACA+TGG 0.505690 1.2:+55526628 MS.gene32079:CDS
GCTAAACCGTCTTTAGCGTA+AGG 0.513178 1.2:-55526039 None:intergenic
ACTCTGAGCGGCGAGCCAAT+TGG 0.513719 1.2:-55527216 None:intergenic
TAAGTATTGGGATACCGAAA+CGG 0.518461 1.2:+55526866 MS.gene32079:CDS
CATGTATGTACCAATCTCTT+CGG 0.518553 1.2:-55526627 None:intergenic
ATCTCAAAACCCTAATTCAC+CGG 0.519645 1.2:+55525834 MS.gene32079:CDS
CAAAGTAGCGACGTGTGGCT+GGG 0.520964 1.2:+55527490 MS.gene32079:CDS
AGGTAGGGTTGAAGGCGATA+AGG 0.521290 1.2:-55525978 None:intergenic
TTGGGATACCGAAACGGGGC+AGG 0.523429 1.2:+55526872 MS.gene32079:CDS
GTAGCGACGTGTGGCTGGGA+CGG 0.525013 1.2:+55527494 MS.gene32079:CDS
GTTATATCGACTTTCTCCAC+TGG 0.528482 1.2:+55526894 MS.gene32079:CDS
TCTTTAGCGTAAGGGTGAGA+TGG 0.528553 1.2:-55526030 None:intergenic
CTGGTCGTAGGTAGGGTTGA+AGG 0.530035 1.2:-55525986 None:intergenic
AAGGCGATAAGGTGTTGAGT+TGG 0.533837 1.2:-55525967 None:intergenic
GAGTACCTTTCATGGGAAAG+AGG 0.534334 1.2:+55526530 MS.gene32079:CDS
CTGTTATATACCGAAGAGAT+TGG 0.537473 1.2:+55526617 MS.gene32079:CDS
CCTGAGGGACTTGTCATCAC+TGG 0.537996 1.2:-55527105 None:intergenic
TGATAAGAAGATTGTTCAGT+GGG 0.538924 1.2:+55526986 MS.gene32079:CDS
CTTTAGCGTAAGGGTGAGAT+GGG 0.540886 1.2:-55526029 None:intergenic
TGTCACGAAGGCGTTACCAT+TGG 0.543297 1.2:+55527440 MS.gene32079:CDS
AAGATAAGAGTACCTTTCAT+GGG 0.546485 1.2:+55526523 MS.gene32079:CDS
TTGGGTCGATGGGTTTGGAG+AGG 0.547307 1.2:-55525948 None:intergenic
CATGGAGTGGACATACGAAA+GGG 0.549842 1.2:+55526646 MS.gene32079:CDS
TGACAAGTCCCTCAGGGTAT+GGG 0.550285 1.2:+55527112 MS.gene32079:CDS
CCTTCCTTCTTCCCAGCCCA+AGG 0.553576 1.2:-55526393 None:intergenic
AAGGTGTTGAGTTGGGTCGA+TGG 0.555828 1.2:-55525959 None:intergenic
AGAATCCGGCTTCCGAGGCG+TGG 0.556053 1.2:+55526349 MS.gene32079:CDS
TGCTGTTCATCAAAGAGGAA+AGG 0.560443 1.2:-55526114 None:intergenic
TGCGCCTCCAAAAGATGCTA+AGG 0.561249 1.2:+55526581 MS.gene32079:CDS
GGGACGGGTTGATAAAGTAC+TGG 0.561357 1.2:+55527510 MS.gene32079:CDS
CGAAAGTAACAAAGACGAAG+AGG 0.562326 1.2:+55526314 MS.gene32079:CDS
CCTTGGGCTGGGAAGAAGGA+AGG 0.564772 1.2:+55526393 MS.gene32079:CDS
GCATGGTGTCGTCGACTTTG+GGG 0.565100 1.2:-55525897 None:intergenic
GGCGACAGTTAGGGCGAGCA+TGG 0.566618 1.2:-55525914 None:intergenic
GGGAAGAAGGAAGGTTTACA+AGG 0.566983 1.2:+55526402 MS.gene32079:CDS
GACATGACAAAACGTCCATC+CGG 0.567963 1.2:-55527350 None:intergenic
TGTCATGTCGGGTGATGGTG+AGG 0.571408 1.2:+55527364 MS.gene32079:CDS
ATTGTTATCGACAAAAGTAA+TGG 0.571823 1.2:-55527066 None:intergenic
AAAGAGGAAAGGGATTATCA+AGG 0.572402 1.2:+55526546 MS.gene32079:CDS
CGGGAAGGGAGGAGACGAGG+TGG 0.573173 1.2:-55525865 None:intergenic
GATCGAGAATCCGGCTTCCG+AGG 0.574103 1.2:+55526344 MS.gene32079:CDS
GTGAGATGGGCCTTGAATCG+GGG 0.578748 1.2:-55526016 None:intergenic
CCTTTCATGGGAAAGAGGAA+AGG 0.585006 1.2:+55526535 MS.gene32079:CDS
CGGCGAGCCAATTGGCATTG+GGG 0.587352 1.2:-55527208 None:intergenic
AAGAGTATGATCAGCATTTG+GGG 0.593826 1.2:+55527033 MS.gene32079:CDS
TTCAGTGGGATATGAATACT+GGG 0.594900 1.2:+55527000 MS.gene32079:CDS
TTATATCGACTTTCTCCACT+GGG 0.596005 1.2:+55526895 MS.gene32079:CDS
GCTACTTTGCTCTGTTCCAG+AGG 0.598337 1.2:-55527476 None:intergenic
AGATACTTATTTATAGCACA+AGG 0.600630 1.2:+55527249 MS.gene32079:CDS
TGTTATGCTGTTCATCAAAG+AGG 0.601244 1.2:-55526119 None:intergenic
GCTAAAGACGGTTTAGCACA+AGG 0.608053 1.2:+55526045 MS.gene32079:CDS
ATTCTTCAACAACCACGCCT+CGG 0.608481 1.2:-55526361 None:intergenic
GCGTTACCATTGGTTGCGAG+TGG 0.608524 1.2:+55527450 MS.gene32079:CDS
AGGAAAGGGATTATCAAGGT+AGG 0.608842 1.2:+55526550 MS.gene32079:CDS
CTAAACCGTCTTTAGCGTAA+GGG 0.610141 1.2:-55526038 None:intergenic
ACATGGAGTGGACATACGAA+AGG 0.611220 1.2:+55526645 MS.gene32079:CDS
GTCATGTCGGGTGATGGTGA+GGG 0.625539 1.2:+55527365 MS.gene32079:CDS
CCGACGTAATTCGAAGCAGA+AGG 0.625730 1.2:-55526171 None:intergenic
CGGGGCCCAGAGCTGGTCGT+AGG 0.625795 1.2:-55525998 None:intergenic
ACCATATTCCCATACCCTGA+GGG 0.627603 1.2:-55527120 None:intergenic
CATGGTGTCGTCGACTTTGG+GGG 0.627690 1.2:-55525896 None:intergenic
CAGTGATGACAAGTCCCTCA+GGG 0.630201 1.2:+55527106 MS.gene32079:CDS
TCTCACCCTTACGCTAAAGA+CGG 0.631080 1.2:+55526033 MS.gene32079:CDS
AGGATGCCACTCGCAACCAA+TGG 0.631424 1.2:-55527456 None:intergenic
TTGGGGTGAAGTGAAATGGA+AGG 0.631463 1.2:-55527191 None:intergenic
AGATTGGTACATACATGGAG+TGG 0.644932 1.2:+55526633 MS.gene32079:CDS
CAGAGCAAAGTAGCGACGTG+TGG 0.656241 1.2:+55527485 MS.gene32079:CDS
GTGATAAGAAGATTGTTCAG+TGG 0.659340 1.2:+55526985 MS.gene32079:CDS
CCTTCTGCTTCGAATTACGT+CGG 0.660337 1.2:+55526171 MS.gene32079:CDS
GAAAGTAACAAAGACGAAGA+GGG 0.665080 1.2:+55526315 MS.gene32079:CDS
AATGTATGAGGACTTATATG+GGG 0.667045 1.2:+55526766 MS.gene32079:CDS
TGTGAAGCTTAACCCAGATG+AGG 0.669296 1.2:+55526932 MS.gene32079:CDS
GATACTTATTTATAGCACAA+GGG 0.672388 1.2:+55527250 MS.gene32079:CDS
GTGATGGTGAGGGTAAGTGT+TGG 0.677988 1.2:+55527375 MS.gene32079:CDS
AGATCAGAAGAAATATGCTG+AGG 0.679573 1.2:+55526437 MS.gene32079:CDS
CTGGTTATCCAAACTCTGAG+CGG 0.682803 1.2:-55527228 None:intergenic
GGTATGCGTAATCACAAACT+CGG 0.683642 1.2:+55526066 MS.gene32079:CDS
ATGGAGTGGACATACGAAAG+GGG 0.691126 1.2:+55526647 MS.gene32079:CDS
AGAACTCTCAAGTGTCACGA+AGG 0.693137 1.2:+55527428 MS.gene32079:CDS
TACCATATTCCCATACCCTG+AGG 0.706671 1.2:-55527121 None:intergenic
GAAGGCATAGAATGCATGTG+TGG 0.724483 1.2:-55527173 None:intergenic
AACATAAGGAATCTTCCCAG+TGG 0.726027 1.2:-55526910 None:intergenic
AGTATTGGGATACCGAAACG+GGG 0.752897 1.2:+55526868 MS.gene32079:CDS
AGAGTATGATCAGCATTTGG+GGG 0.760214 1.2:+55527034 MS.gene32079:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACGATAAGAATATTAAGTAT+TGG + chr1.2:55526853-55526872 MS.gene32079:CDS 20.0%
!! CGATAAGAATATTAAGTATT+GGG + chr1.2:55526854-55526873 MS.gene32079:CDS 20.0%
!!! CATAACACCTTTTTAAAATT+CGG + chr1.2:55526135-55526154 MS.gene32079:CDS 20.0%
!!! GATATTTGTTTTACAAATGA+TGG + chr1.2:55526807-55526826 MS.gene32079:CDS 20.0%
! AAATGTATGAGGACTTATAT+GGG + chr1.2:55526765-55526784 MS.gene32079:CDS 25.0%
! AGATACTTATTTATAGCACA+AGG + chr1.2:55527249-55527268 MS.gene32079:CDS 25.0%
! ATTGTTATCGACAAAAGTAA+TGG - chr1.2:55527069-55527088 None:intergenic 25.0%
! CTGATATACTTTATAACTAC+AGG - chr1.2:55527149-55527168 None:intergenic 25.0%
! GATACTTATTTATAGCACAA+GGG + chr1.2:55527250-55527269 MS.gene32079:CDS 25.0%
! GGATACTAAGGTTAAAATTT+GGG + chr1.2:55526725-55526744 MS.gene32079:CDS 25.0%
! TAAATGTATGAGGACTTATA+TGG + chr1.2:55526764-55526783 MS.gene32079:CDS 25.0%
! TGGATACTAAGGTTAAAATT+TGG + chr1.2:55526724-55526743 MS.gene32079:CDS 25.0%
! TTCAACTTAACAAAAAGAAG+AGG + chr1.2:55527279-55527298 MS.gene32079:CDS 25.0%
! TTGTGCTATAAATAAGTATC+TGG - chr1.2:55527250-55527269 None:intergenic 25.0%
!!! TTACTTTTGTCGATAACAAT+CGG + chr1.2:55527069-55527088 MS.gene32079:CDS 25.0%
AAGAAAGGTGAAGAAAAAAG+TGG + chr1.2:55526471-55526490 MS.gene32079:CDS 30.0%
AATGTATGAGGACTTATATG+GGG + chr1.2:55526766-55526785 MS.gene32079:CDS 30.0%
GAAGAATAAAAAGAGTCCTT+GGG + chr1.2:55526377-55526396 MS.gene32079:CDS 30.0%
TCAATACTGGTAAATGTATG+AGG + chr1.2:55526754-55526773 MS.gene32079:CDS 30.0%
TGAAGAATAAAAAGAGTCCT+TGG + chr1.2:55526376-55526395 MS.gene32079:CDS 30.0%
TGATAAGAAGATTGTTCAGT+GGG + chr1.2:55526986-55527005 MS.gene32079:CDS 30.0%
! GGATAAACAGAATGTTTTGT+TGG + chr1.2:55526953-55526972 MS.gene32079:CDS 30.0%
! TTTTTCTCTTCTTCTGTTCA+CGG - chr1.2:55526251-55526270 None:intergenic 30.0%
!! AAAGATAAGAGTACCTTTCA+TGG + chr1.2:55526522-55526541 MS.gene32079:CDS 30.0%
!! AAGATAAGAGTACCTTTCAT+GGG + chr1.2:55526523-55526542 MS.gene32079:CDS 30.0%
!! AGCGTAACCGAATTTTAAAA+AGG - chr1.2:55526145-55526164 None:intergenic 30.0%
!! ATTTGGGATGTTTTCAATAC+TGG + chr1.2:55526741-55526760 MS.gene32079:CDS 30.0%
!!! ATTAGGTTTTTTCCGAATTC+GGG + chr1.2:55526678-55526697 MS.gene32079:CDS 30.0%
!!! GATTAGGTTTTTTCCGAATT+CGG + chr1.2:55526677-55526696 MS.gene32079:CDS 30.0%
AAAGAGGAAAGGGATTATCA+AGG + chr1.2:55526546-55526565 MS.gene32079:CDS 35.0%
AACAAAAAGAAGAGGTTTGC+TGG + chr1.2:55527287-55527306 MS.gene32079:CDS 35.0%
ACATTCTGTTTATCCTCATC+TGG - chr1.2:55526948-55526967 None:intergenic 35.0%
AGAATCAAATGACCCGAATT+CGG - chr1.2:55526693-55526712 None:intergenic 35.0%
AGATCAGAAGAAATATGCTG+AGG + chr1.2:55526437-55526456 MS.gene32079:CDS 35.0%
ATCTCAAAACCCTAATTCAC+CGG + chr1.2:55525834-55525853 MS.gene32079:CDS 35.0%
CATGTATGTACCAATCTCTT+CGG - chr1.2:55526630-55526649 None:intergenic 35.0%
CATTCTGTTTATCCTCATCT+GGG - chr1.2:55526947-55526966 None:intergenic 35.0%
CGAATCTCTTAAGAAGAAAG+AGG + chr1.2:55526272-55526291 MS.gene32079:CDS 35.0%
CTGTTATATACCGAAGAGAT+TGG + chr1.2:55526617-55526636 MS.gene32079:CDS 35.0%
GAAAGTAACAAAGACGAAGA+GGG + chr1.2:55526315-55526334 MS.gene32079:CDS 35.0%
GTGATAAGAAGATTGTTCAG+TGG + chr1.2:55526985-55527004 MS.gene32079:CDS 35.0%
TGTTATGCTGTTCATCAAAG+AGG - chr1.2:55526122-55526141 None:intergenic 35.0%
TTCAGTGGGATATGAATACT+GGG + chr1.2:55527000-55527019 MS.gene32079:CDS 35.0%
! AAGAGTATGATCAGCATTTG+GGG + chr1.2:55527033-55527052 MS.gene32079:CDS 35.0%
! CAAGAGTATGATCAGCATTT+GGG + chr1.2:55527032-55527051 MS.gene32079:CDS 35.0%
! GAGAATTTTCCGGTGAATTA+GGG - chr1.2:55525846-55525865 None:intergenic 35.0%
! GGTGAAGAAAAAAGTGGTTT+TGG + chr1.2:55526477-55526496 MS.gene32079:CDS 35.0%
! TCAAGAGTATGATCAGCATT+TGG + chr1.2:55527031-55527050 MS.gene32079:CDS 35.0%
!! AAACAGAATGTTTTGTTGGC+TGG + chr1.2:55526957-55526976 MS.gene32079:CDS 35.0%
!! TAAGTATTGGGATACCGAAA+CGG + chr1.2:55526866-55526885 MS.gene32079:CDS 35.0%
!! TTATATCGACTTTCTCCACT+GGG + chr1.2:55526895-55526914 MS.gene32079:CDS 35.0%
!!! GAAGAAAAAAGTGGTTTTGG+TGG + chr1.2:55526480-55526499 MS.gene32079:CDS 35.0%
!!! TTTTGGTGGTGAAAAAGTTG+AGG + chr1.2:55526494-55526513 MS.gene32079:CDS 35.0%
AACATAAGGAATCTTCCCAG+TGG - chr1.2:55526913-55526932 None:intergenic 40.0%
AATAAAAAGAGTCCTTGGGC+TGG + chr1.2:55526381-55526400 MS.gene32079:CDS 40.0%
AGAGTATGATCAGCATTTGG+GGG + chr1.2:55527034-55527053 MS.gene32079:CDS 40.0%
AGATTGGTACATACATGGAG+TGG + chr1.2:55526633-55526652 MS.gene32079:CDS 40.0%
AGGAAAGGGATTATCAAGGT+AGG + chr1.2:55526550-55526569 MS.gene32079:CDS 40.0%
ATAAAAAGAGTCCTTGGGCT+GGG + chr1.2:55526382-55526401 MS.gene32079:CDS 40.0%
CGAAAGTAACAAAGACGAAG+AGG + chr1.2:55526314-55526333 MS.gene32079:CDS 40.0%
CGAAGAGATTGGTACATACA+TGG + chr1.2:55526628-55526647 MS.gene32079:CDS 40.0%
CTAAACCGTCTTTAGCGTAA+GGG - chr1.2:55526041-55526060 None:intergenic 40.0%
CTTTCATGGGAAAGAGGAAA+GGG + chr1.2:55526536-55526555 MS.gene32079:CDS 40.0%
GGGTTAAGCTTCACAACATA+AGG - chr1.2:55526927-55526946 None:intergenic 40.0%
GGTATGCGTAATCACAAACT+CGG + chr1.2:55526066-55526085 MS.gene32079:CDS 40.0%
GTTCAGTGGGATATGAATAC+TGG + chr1.2:55526999-55527018 MS.gene32079:CDS 40.0%
TGCTGTTCATCAAAGAGGAA+AGG - chr1.2:55526117-55526136 None:intergenic 40.0%
TTCGGGTCATTTGATTCTCT+CGG + chr1.2:55526695-55526714 MS.gene32079:CDS 40.0%
TTTGCTGGACATATTGTTGC+TGG + chr1.2:55527302-55527321 MS.gene32079:CDS 40.0%
! GGAGAATTTTCCGGTGAATT+AGG - chr1.2:55525847-55525866 None:intergenic 40.0%
! TTACTCGCCTTAGCATCTTT+TGG - chr1.2:55526591-55526610 None:intergenic 40.0%
! TTGTCAGGTCAATTTTTCGC+CGG + chr1.2:55527331-55527350 MS.gene32079:CDS 40.0%
!! AAGTATTGGGATACCGAAAC+GGG + chr1.2:55526867-55526886 MS.gene32079:CDS 40.0%
!! GTTATATCGACTTTCTCCAC+TGG + chr1.2:55526894-55526913 MS.gene32079:CDS 40.0%
!!! GGAACTAAGTTTTTGAGTGC+TGG + chr1.2:55526828-55526847 MS.gene32079:CDS 40.0%
!!! GGTGAATTAGGGTTTTGAGA+TGG - chr1.2:55525835-55525854 None:intergenic 40.0%
ACATGGAGTGGACATACGAA+AGG + chr1.2:55526645-55526664 MS.gene32079:CDS 45.0%
ACCATATTCCCATACCCTGA+GGG - chr1.2:55527123-55527142 None:intergenic 45.0%
AGAACTCTCAAGTGTCACGA+AGG + chr1.2:55527428-55527447 MS.gene32079:CDS 45.0%
ATGGAGTGGACATACGAAAG+GGG + chr1.2:55526647-55526666 MS.gene32079:CDS 45.0%
ATTCTTCAACAACCACGCCT+CGG - chr1.2:55526364-55526383 None:intergenic 45.0%
CATGGAGTGGACATACGAAA+GGG + chr1.2:55526646-55526665 MS.gene32079:CDS 45.0%
CCTTCTGCTTCGAATTACGT+CGG + chr1.2:55526171-55526190 MS.gene32079:CDS 45.0%
CCTTTCATGGGAAAGAGGAA+AGG + chr1.2:55526535-55526554 MS.gene32079:CDS 45.0%
CCTTTCCTCTTTCCCATGAA+AGG - chr1.2:55526538-55526557 None:intergenic 45.0%
CTGGTTATCCAAACTCTGAG+CGG - chr1.2:55527231-55527250 None:intergenic 45.0%
CTTTAGCGTAAGGGTGAGAT+GGG - chr1.2:55526032-55526051 None:intergenic 45.0%
GACATGACAAAACGTCCATC+CGG - chr1.2:55527353-55527372 None:intergenic 45.0%
GAGTACCTTTCATGGGAAAG+AGG + chr1.2:55526530-55526549 MS.gene32079:CDS 45.0%
GCTAAACCGTCTTTAGCGTA+AGG - chr1.2:55526042-55526061 None:intergenic 45.0%
GCTAAAGACGGTTTAGCACA+AGG + chr1.2:55526045-55526064 MS.gene32079:CDS 45.0%
GGGAAGAAGGAAGGTTTACA+AGG + chr1.2:55526402-55526421 MS.gene32079:CDS 45.0%
GGGATTATCAAGGTAGGTCT+TGG + chr1.2:55526556-55526575 MS.gene32079:CDS 45.0%
TACCATATTCCCATACCCTG+AGG - chr1.2:55527124-55527143 None:intergenic 45.0%
TCTCACCCTTACGCTAAAGA+CGG + chr1.2:55526033-55526052 MS.gene32079:CDS 45.0%
TCTTCTTCTGTTCACGGTGT+GGG - chr1.2:55526245-55526264 None:intergenic 45.0%
TCTTTAGCGTAAGGGTGAGA+TGG - chr1.2:55526033-55526052 None:intergenic 45.0%
TGGATCGTTGTCGTTGTTGT+CGG - chr1.2:55525806-55525825 None:intergenic 45.0%
TGTCGTTGTTGTCGGTGTAT+TGG - chr1.2:55525798-55525817 None:intergenic 45.0%
TGTGAAGCTTAACCCAGATG+AGG + chr1.2:55526932-55526951 MS.gene32079:CDS 45.0%
TGTTGCTGGATATGCTTGTC+AGG + chr1.2:55527316-55527335 MS.gene32079:CDS 45.0%
TTGGGGTGAAGTGAAATGGA+AGG - chr1.2:55527194-55527213 None:intergenic 45.0%
TTTGATTCTCTCGGCTGGTA+TGG + chr1.2:55526704-55526723 MS.gene32079:CDS 45.0%
! AGACGAGGTGGAGAATTTTC+CGG - chr1.2:55525856-55525875 None:intergenic 45.0%
! AGTATTGGGATACCGAAACG+GGG + chr1.2:55526868-55526887 MS.gene32079:CDS 45.0%
! ATGGGTTTGGAGAGGGTTTT+GGG - chr1.2:55525943-55525962 None:intergenic 45.0%
! GAAGGCATAGAATGCATGTG+TGG - chr1.2:55527176-55527195 None:intergenic 45.0%
! GAGGAGCACGCAAAAAAGAA+AGG + chr1.2:55526456-55526475 MS.gene32079:CDS 45.0%
! GATGGACGTTTTGTCATGTC+GGG + chr1.2:55527353-55527372 MS.gene32079:CDS 45.0%
! GGATGGACGTTTTGTCATGT+CGG + chr1.2:55527352-55527371 MS.gene32079:CDS 45.0%
!! AAGGCGATAAGGTGTTGAGT+TGG - chr1.2:55525970-55525989 None:intergenic 45.0%
!! AGGCGATAAGGTGTTGAGTT+GGG - chr1.2:55525969-55525988 None:intergenic 45.0%
!! GTAAGTGTTGGTTCTGGGAT+TGG + chr1.2:55527387-55527406 MS.gene32079:CDS 45.0%
!! TGAGGGTAAGTGTTGGTTCT+GGG + chr1.2:55527382-55527401 MS.gene32079:CDS 45.0%
AGGTAGGGTTGAAGGCGATA+AGG - chr1.2:55525981-55526000 None:intergenic 50.0%
ATGACAAGTCCCTCAGGGTA+TGG + chr1.2:55527111-55527130 MS.gene32079:CDS 50.0%
CACTTCACCCCAATGCCAAT+TGG + chr1.2:55527201-55527220 MS.gene32079:CDS 50.0%
CAGTGATGACAAGTCCCTCA+GGG + chr1.2:55527106-55527125 MS.gene32079:CDS 50.0%
CCGACGTAATTCGAAGCAGA+AGG - chr1.2:55526174-55526193 None:intergenic 50.0%
CGAAAGGGGTTTCTGCGATT+AGG + chr1.2:55526661-55526680 MS.gene32079:CDS 50.0%
CGATATAACCTGCCCCGTTT+CGG - chr1.2:55526883-55526902 None:intergenic 50.0%
CTCGGCTGGTATGGATACTA+AGG + chr1.2:55526713-55526732 MS.gene32079:CDS 50.0%
CTCTTCTTCTGTTCACGGTG+TGG - chr1.2:55526246-55526265 None:intergenic 50.0%
GGTCATTTGATTCTCTCGGC+TGG + chr1.2:55526699-55526718 MS.gene32079:CDS 50.0%
GTATGATCAGCATTTGGGGG+CGG + chr1.2:55527037-55527056 MS.gene32079:CDS 50.0%
TCCCTCAGGGTATGGGAATA+TGG + chr1.2:55527119-55527138 MS.gene32079:CDS 50.0%
TGACAAGTCCCTCAGGGTAT+GGG + chr1.2:55527112-55527131 MS.gene32079:CDS 50.0%
TGCGCCTCCAAAAGATGCTA+AGG + chr1.2:55526581-55526600 MS.gene32079:CDS 50.0%
! CAGGTCAATTTTTCGCCGGA+TGG + chr1.2:55527335-55527354 MS.gene32079:CDS 50.0%
! CGTTTTGTCATGTCGGGTGA+TGG + chr1.2:55527359-55527378 MS.gene32079:CDS 50.0%
! CTCGCCTTAGCATCTTTTGG+AGG - chr1.2:55526588-55526607 None:intergenic 50.0%
! GCTACTTTGCTCTGTTCCAG+AGG - chr1.2:55527479-55527498 None:intergenic 50.0%
! GGCATTGGGGTGAAGTGAAA+TGG - chr1.2:55527198-55527217 None:intergenic 50.0%
! GGGGCATTCGAAAGCTGTTA+GGG + chr1.2:55526785-55526804 MS.gene32079:CDS 50.0%
! GTGATGGTGAGGGTAAGTGT+TGG + chr1.2:55527375-55527394 MS.gene32079:CDS 50.0%
! TGGGGCATTCGAAAGCTGTT+AGG + chr1.2:55526784-55526803 MS.gene32079:CDS 50.0%
! TGGGTTTGGAGAGGGTTTTG+GGG - chr1.2:55525942-55525961 None:intergenic 50.0%
!! AAGGTGTTGAGTTGGGTCGA+TGG - chr1.2:55525962-55525981 None:intergenic 50.0%
!! AGCATGGTGTCGTCGACTTT+GGG - chr1.2:55525901-55525920 None:intergenic 50.0%
!! AGGTGTTGAGTTGGGTCGAT+GGG - chr1.2:55525961-55525980 None:intergenic 50.0%
!! GATGGGTTTGGAGAGGGTTT+TGG - chr1.2:55525944-55525963 None:intergenic 50.0%
!! GGGACGGGTTGATAAAGTAC+TGG + chr1.2:55527510-55527529 MS.gene32079:CDS 50.0%
!! GGGTGTTGAGATCGAGAATC+CGG + chr1.2:55526335-55526354 MS.gene32079:CDS 50.0%
!! GTGAGGGTAAGTGTTGGTTC+TGG + chr1.2:55527381-55527400 MS.gene32079:CDS 50.0%
!! TGTCACGAAGGCGTTACCAT+TGG + chr1.2:55527440-55527459 MS.gene32079:CDS 50.0%
!! TTGAGTTGGGTCGATGGGTT+TGG - chr1.2:55525956-55525975 None:intergenic 50.0%
!!! GGGTTTTGAGATGGTGAGTC+TGG - chr1.2:55525826-55525845 None:intergenic 50.0%
!!! TTTGGAGAGGGTTTTGGGGT+CGG - chr1.2:55525938-55525957 None:intergenic 50.0%
AGGATGCCACTCGCAACCAA+TGG - chr1.2:55527459-55527478 None:intergenic 55.0%
CAAAGTAGCGACGTGTGGCT+GGG + chr1.2:55527490-55527509 MS.gene32079:CDS 55.0%
CAGAGCAAAGTAGCGACGTG+TGG + chr1.2:55527485-55527504 MS.gene32079:CDS 55.0%
CCAGTGATGACAAGTCCCTC+AGG + chr1.2:55527105-55527124 MS.gene32079:CDS 55.0%
CCTGAGGGACTTGTCATCAC+TGG - chr1.2:55527108-55527127 None:intergenic 55.0%
CTGGTCGTAGGTAGGGTTGA+AGG - chr1.2:55525989-55526008 None:intergenic 55.0%
GGGTGAGATGGGCCTTGAAT+CGG - chr1.2:55526021-55526040 None:intergenic 55.0%
GGTGAGATGGGCCTTGAATC+GGG - chr1.2:55526020-55526039 None:intergenic 55.0%
GTGAGATGGGCCTTGAATCG+GGG - chr1.2:55526019-55526038 None:intergenic 55.0%
! GCGTTACCATTGGTTGCGAG+TGG + chr1.2:55527450-55527469 MS.gene32079:CDS 55.0%
! GTCATGTCGGGTGATGGTGA+GGG + chr1.2:55527365-55527384 MS.gene32079:CDS 55.0%
! TGGGTCGATGGGTTTGGAGA+GGG - chr1.2:55525950-55525969 None:intergenic 55.0%
! TGTCATGTCGGGTGATGGTG+AGG + chr1.2:55527364-55527383 MS.gene32079:CDS 55.0%
! TTGGGTCGATGGGTTTGGAG+AGG - chr1.2:55525951-55525970 None:intergenic 55.0%
! TTTGGGGTCGGCGACAGTTA+GGG - chr1.2:55525926-55525945 None:intergenic 55.0%
!! CATGGTGTCGTCGACTTTGG+GGG - chr1.2:55525899-55525918 None:intergenic 55.0%
!! GAGCATGGTGTCGTCGACTT+TGG - chr1.2:55525902-55525921 None:intergenic 55.0%
!! GCATGGTGTCGTCGACTTTG+GGG - chr1.2:55525900-55525919 None:intergenic 55.0%
!!! TTTTGGGGTCGGCGACAGTT+AGG - chr1.2:55525927-55525946 None:intergenic 55.0%
AACAACCACGCCTCGGAAGC+CGG - chr1.2:55526357-55526376 None:intergenic 60.0%
ACCCTACCTACGACCAGCTC+TGG + chr1.2:55525992-55526011 MS.gene32079:CDS 60.0%
ACTCTGAGCGGCGAGCCAAT+TGG - chr1.2:55527219-55527238 None:intergenic 60.0%
AGCGGCGAGCCAATTGGCAT+TGG - chr1.2:55527213-55527232 None:intergenic 60.0%
CCCAGAGCTGGTCGTAGGTA+GGG - chr1.2:55525996-55526015 None:intergenic 60.0%
CCCTACCTACGACCAGCTCT+GGG + chr1.2:55525993-55526012 MS.gene32079:CDS 60.0%
CCTTCCTTCTTCCCAGCCCA+AGG - chr1.2:55526396-55526415 None:intergenic 60.0%
CGGCGAGCCAATTGGCATTG+GGG - chr1.2:55527211-55527230 None:intergenic 60.0%
GATCGAGAATCCGGCTTCCG+AGG + chr1.2:55526344-55526363 MS.gene32079:CDS 60.0%
GCAAAGTAGCGACGTGTGGC+TGG + chr1.2:55527489-55527508 MS.gene32079:CDS 60.0%
GCGGCGAGCCAATTGGCATT+GGG - chr1.2:55527212-55527231 None:intergenic 60.0%
TGGTTGCGAGTGGCATCCTC+TGG + chr1.2:55527460-55527479 MS.gene32079:CDS 60.0%
TTGGGATACCGAAACGGGGC+AGG + chr1.2:55526872-55526891 MS.gene32079:CDS 60.0%
! CCTTGGGCTGGGAAGAAGGA+AGG + chr1.2:55526393-55526412 MS.gene32079:CDS 60.0%
! GAGTCCTTGGGCTGGGAAGA+AGG + chr1.2:55526389-55526408 MS.gene32079:CDS 60.0%
! TTGGCTCGCCGCTCAGAGTT+TGG + chr1.2:55527220-55527239 MS.gene32079:CDS 60.0%
AGAATCCGGCTTCCGAGGCG+TGG + chr1.2:55526349-55526368 MS.gene32079:CDS 65.0%
CTTGAATCGGGGCCCAGAGC+TGG - chr1.2:55526008-55526027 None:intergenic 65.0%
GAACGGGAAGGGAGGAGACG+AGG - chr1.2:55525871-55525890 None:intergenic 65.0%
GCCCAGAGCTGGTCGTAGGT+AGG - chr1.2:55525997-55526016 None:intergenic 65.0%
GGCGACAGTTAGGGCGAGCA+TGG - chr1.2:55525917-55525936 None:intergenic 65.0%
GTAGCGACGTGTGGCTGGGA+CGG + chr1.2:55527494-55527513 MS.gene32079:CDS 65.0%
TAGCGACGTGTGGCTGGGAC+GGG + chr1.2:55527495-55527514 MS.gene32079:CDS 65.0%
!! CAGCTCTGGGCCCCGATTCA+AGG + chr1.2:55526006-55526025 MS.gene32079:CDS 65.0%
!! GGTGTCGTCGACTTTGGGGG+CGG - chr1.2:55525896-55525915 None:intergenic 65.0%
CGGGAAGGGAGGAGACGAGG+TGG - chr1.2:55525868-55525887 None:intergenic 70.0%
! CGACTTTGGGGGCGGCGGAA+CGG - chr1.2:55525888-55525907 None:intergenic 70.0%
! GACTTTGGGGGCGGCGGAAC+GGG - chr1.2:55525887-55525906 None:intergenic 70.0%
!! GTCGTCGACTTTGGGGGCGG+CGG - chr1.2:55525893-55525912 None:intergenic 70.0%
CGGGGCCCAGAGCTGGTCGT+AGG - chr1.2:55526001-55526020 None:intergenic 75.0%
TGGGGGCGGCGGAACGGGAA+GGG - chr1.2:55525882-55525901 None:intergenic 75.0%
TTGGGGGCGGCGGAACGGGA+AGG - chr1.2:55525883-55525902 None:intergenic 75.0%
! GGGCGGCGGAACGGGAAGGG+AGG - chr1.2:55525879-55525898 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 55525781 55527535 55525781 ID=MS.gene32079
chr1.2 mRNA 55525781 55527535 55525781 ID=MS.gene32079.t1;Parent=MS.gene32079
chr1.2 exon 55525781 55527535 55525781 ID=MS.gene32079.t1.exon1;Parent=MS.gene32079.t1
chr1.2 CDS 55525781 55527535 55525781 ID=cds.MS.gene32079.t1;Parent=MS.gene32079.t1
Gene Sequence

>MS.gene32079

ATGGATCTACTCAACCAATACACCGACAACAACGACAACGATCCAGACTCACCATCTCAAAACCCTAATTCACCGGAAAATTCTCCACCTCGTCTCCTCCCTTCCCGTTCCGCCGCCCCCAAAGTCGACGACACCATGCTCGCCCTAACTGTCGCCGACCCCAAAACCCTCTCCAAACCCATCGACCCAACTCAACACCTTATCGCCTTCAACCCTACCTACGACCAGCTCTGGGCCCCGATTCAAGGCCCATCTCACCCTTACGCTAAAGACGGTTTAGCACAAGGTATGCGTAATCACAAACTCGGTTTTGTCGAAGACGCTAATATCGAACCTTTCCTCTTTGATGAACAGCATAACACCTTTTTAAAATTCGGTTACGCTGCTGATCCTTCTGCTTCGAATTACGTCGGTGATTTTGATGCTTTGAAGAAGAATAATGCTGCTTCTGTTTATAATATCCCACACCGTGAACAGAAGAAGAGAAAAATCGAATCTCTTAAGAAGAAAGAGGAGAATGATAACGAAGAAGACGAAAGTAACAAAGACGAAGAGGGTGTTGAGATCGAGAATCCGGCTTCCGAGGCGTGGTTGTTGAAGAATAAAAAGAGTCCTTGGGCTGGGAAGAAGGAAGGTTTACAAGGAGAGTTAACTGAAGATCAGAAGAAATATGCTGAGGAGCACGCAAAAAAGAAAGGTGAAGAAAAAAGTGGTTTTGGTGGTGAAAAAGTTGAGGCTGTTAAAGATAAGAGTACCTTTCATGGGAAAGAGGAAAGGGATTATCAAGGTAGGTCTTGGATTGCGCCTCCAAAAGATGCTAAGGCGAGTAATGATCACTGTTATATACCGAAGAGATTGGTACATACATGGAGTGGACATACGAAAGGGGTTTCTGCGATTAGGTTTTTTCCGAATTCGGGTCATTTGATTCTCTCGGCTGGTATGGATACTAAGGTTAAAATTTGGGATGTTTTCAATACTGGTAAATGTATGAGGACTTATATGGGGCATTCGAAAGCTGTTAGGGATATTTGTTTTACAAATGATGGAACTAAGTTTTTGAGTGCTGGTTACGATAAGAATATTAAGTATTGGGATACCGAAACGGGGCAGGTTATATCGACTTTCTCCACTGGGAAGATTCCTTATGTTGTGAAGCTTAACCCAGATGAGGATAAACAGAATGTTTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATACTGGGCAGATCACTCAAGAGTATGATCAGCATTTGGGGGCGGTGAATACCATTACTTTTGTCGATAACAATCGGAGATTTGTTACTTCCAGTGATGACAAGTCCCTCAGGGTATGGGAATATGGTATTCCTGTAGTTATAAAGTATATCAGTGAGCCACACATGCATTCTATGCCTTCCATTTCACTTCACCCCAATGCCAATTGGCTCGCCGCTCAGAGTTTGGATAACCAGATACTTATTTATAGCACAAGGGAGAAGTTTCAACTTAACAAAAAGAAGAGGTTTGCTGGACATATTGTTGCTGGATATGCTTGTCAGGTCAATTTTTCGCCGGATGGACGTTTTGTCATGTCGGGTGATGGTGAGGGTAAGTGTTGGTTCTGGGATTGGAAGAGTTGCAAAGTCTTTAGAACTCTCAAGTGTCACGAAGGCGTTACCATTGGTTGCGAGTGGCATCCTCTGGAACAGAGCAAAGTAGCGACGTGTGGCTGGGACGGGTTGATAAAGTACTGGTAA
Protein sequence

>MS.gene32079.t1

MDLLNQYTDNNDNDPDSPSQNPNSPENSPPRLLPSRSAAPKVDDTMLALTVADPKTLSKPIDPTQHLIAFNPTYDQLWAPIQGPSHPYAKDGLAQGMRNHKLGFVEDANIEPFLFDEQHNTFLKFGYAADPSASNYVGDFDALKKNNAASVYNIPHREQKKRKIESLKKKEENDNEEDESNKDEEGVEIENPASEAWLLKNKKSPWAGKKEGLQGELTEDQKKYAEEHAKKKGEEKSGFGGEKVEAVKDKSTFHGKEERDYQGRSWIAPPKDAKASNDHCYIPKRLVHTWSGHTKGVSAIRFFPNSGHLILSAGMDTKVKIWDVFNTGKCMRTYMGHSKAVRDICFTNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDEDKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEYGIPVVIKYISEPHMHSMPSISLHPNANWLAAQSLDNQILIYSTREKFQLNKKKRFAGHIVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVTIGCEWHPLEQSKVATCGWDGLIKYW