Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32208.t1 | XP_003608243.1 | 91.7 | 470 | 39 | 0 | 1 | 470 | 59 | 528 | 7.50E-260 | 906 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32208.t1 | Q9FZC5 | 55.9 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 5.2e-153 | 542.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32208.t1 | Q2HTY4 | 91.7 | 470 | 39 | 0 | 1 | 470 | 59 | 528 | 5.4e-260 | 906.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05156 | MS.gene32208 | 0.803842 | 2.87E-49 | -1.69E-46 |
| MS.gene052053 | MS.gene32208 | 0.807016 | 6.21E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene32208 | 0.904936 | 7.28E-80 | -1.69E-46 |
| MS.gene052560 | MS.gene32208 | 0.981742 | 2.46E-153 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32208.t1 | MTR_4g091200 | 91.702 | 470 | 39 | 0 | 1 | 470 | 59 | 528 | 0.0 | 884 |
| MS.gene32208.t1 | MTR_6g017205 | 90.466 | 472 | 45 | 0 | 1 | 472 | 59 | 530 | 0.0 | 866 |
| MS.gene32208.t1 | MTR_4g057990 | 82.516 | 469 | 78 | 2 | 1 | 469 | 59 | 523 | 0.0 | 781 |
| MS.gene32208.t1 | MTR_4g091190 | 79.915 | 468 | 92 | 1 | 1 | 468 | 63 | 528 | 0.0 | 763 |
| MS.gene32208.t1 | MTR_4g088355 | 78.891 | 469 | 99 | 0 | 1 | 469 | 66 | 534 | 0.0 | 761 |
| MS.gene32208.t1 | MTR_2g023590 | 79.070 | 473 | 97 | 1 | 1 | 473 | 1 | 471 | 0.0 | 755 |
| MS.gene32208.t1 | MTR_4g091170 | 73.420 | 459 | 104 | 1 | 10 | 468 | 102 | 542 | 0.0 | 709 |
| MS.gene32208.t1 | MTR_4g091150 | 63.462 | 468 | 169 | 2 | 1 | 466 | 71 | 538 | 0.0 | 598 |
| MS.gene32208.t1 | MTR_2g031560 | 54.292 | 466 | 210 | 2 | 3 | 467 | 59 | 522 | 0.0 | 542 |
| MS.gene32208.t1 | MTR_2g031590 | 53.503 | 471 | 210 | 4 | 3 | 467 | 64 | 531 | 1.76e-179 | 513 |
| MS.gene32208.t1 | MTR_4g094498 | 53.548 | 465 | 212 | 3 | 3 | 467 | 73 | 533 | 2.42e-173 | 498 |
| MS.gene32208.t1 | MTR_4g094488 | 53.911 | 473 | 209 | 4 | 3 | 468 | 67 | 537 | 7.82e-166 | 479 |
| MS.gene32208.t1 | MTR_4g094495 | 52.542 | 472 | 215 | 4 | 4 | 468 | 71 | 540 | 1.09e-164 | 476 |
| MS.gene32208.t1 | MTR_2g031390 | 49.679 | 467 | 227 | 6 | 4 | 466 | 65 | 527 | 6.56e-164 | 474 |
| MS.gene32208.t1 | MTR_4g094492 | 51.173 | 469 | 223 | 3 | 4 | 467 | 68 | 535 | 3.58e-163 | 472 |
| MS.gene32208.t1 | MTR_4g094480 | 52.026 | 469 | 220 | 3 | 4 | 468 | 68 | 535 | 1.35e-162 | 471 |
| MS.gene32208.t1 | MTR_4g094478 | 52.778 | 468 | 216 | 3 | 4 | 467 | 68 | 534 | 1.35e-162 | 471 |
| MS.gene32208.t1 | MTR_4g094520 | 51.173 | 469 | 218 | 6 | 4 | 467 | 69 | 531 | 3.65e-160 | 464 |
| MS.gene32208.t1 | MTR_3g009820 | 48.707 | 464 | 231 | 5 | 4 | 466 | 63 | 520 | 9.89e-160 | 463 |
| MS.gene32208.t1 | MTR_2g031490 | 51.277 | 470 | 219 | 6 | 3 | 466 | 71 | 536 | 4.61e-158 | 459 |
| MS.gene32208.t1 | MTR_4g094518 | 49.893 | 469 | 221 | 7 | 5 | 466 | 67 | 528 | 3.16e-157 | 457 |
| MS.gene32208.t1 | MTR_2g031510 | 49.895 | 475 | 218 | 6 | 3 | 466 | 73 | 538 | 1.02e-155 | 453 |
| MS.gene32208.t1 | MTR_3g009760 | 47.629 | 464 | 236 | 5 | 4 | 466 | 63 | 520 | 1.57e-153 | 447 |
| MS.gene32208.t1 | MTR_2g031420 | 50.855 | 468 | 223 | 4 | 3 | 469 | 67 | 528 | 2.25e-153 | 447 |
| MS.gene32208.t1 | MTR_3g009880 | 48.707 | 464 | 231 | 5 | 4 | 466 | 61 | 518 | 1.63e-152 | 444 |
| MS.gene32208.t1 | MTR_4g094418 | 50.569 | 439 | 209 | 4 | 40 | 471 | 1 | 438 | 2.17e-152 | 447 |
| MS.gene32208.t1 | MTR_2g031460 | 49.569 | 464 | 227 | 4 | 3 | 465 | 67 | 524 | 1.54e-151 | 442 |
| MS.gene32208.t1 | MTR_4g094515 | 51.805 | 471 | 218 | 6 | 3 | 468 | 74 | 540 | 2.91e-150 | 457 |
| MS.gene32208.t1 | MTR_4g094515 | 51.709 | 468 | 219 | 5 | 3 | 467 | 686 | 1149 | 2.45e-148 | 452 |
| MS.gene32208.t1 | MTR_1g076830 | 37.917 | 480 | 282 | 8 | 3 | 473 | 68 | 540 | 2.99e-101 | 314 |
| MS.gene32208.t1 | MTR_2g031600 | 64.444 | 180 | 64 | 0 | 3 | 182 | 65 | 244 | 2.65e-71 | 232 |
| MS.gene32208.t1 | MTR_2g031600 | 51.875 | 160 | 76 | 1 | 308 | 467 | 241 | 399 | 2.94e-53 | 184 |
| MS.gene32208.t1 | MTR_3g009850 | 48.661 | 224 | 112 | 3 | 244 | 466 | 5 | 226 | 9.21e-65 | 209 |
| MS.gene32208.t1 | MTR_2g084370 | 46.847 | 111 | 37 | 2 | 64 | 174 | 1 | 89 | 5.71e-29 | 110 |
| MS.gene32208.t1 | MTR_2g084405 | 45.570 | 79 | 43 | 0 | 64 | 142 | 1 | 79 | 3.95e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32208.t1 | AT4G20800 | 55.579 | 466 | 204 | 2 | 4 | 469 | 64 | 526 | 3.32e-178 | 510 |
| MS.gene32208.t1 | AT1G26420 | 55.172 | 464 | 202 | 4 | 4 | 466 | 65 | 523 | 2.13e-176 | 505 |
| MS.gene32208.t1 | AT1G26380 | 55.388 | 464 | 201 | 4 | 4 | 466 | 65 | 523 | 1.94e-175 | 503 |
| MS.gene32208.t1 | AT1G26390 | 55.914 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 1.03e-174 | 501 |
| MS.gene32208.t1 | AT1G30720 | 53.846 | 468 | 211 | 3 | 2 | 468 | 63 | 526 | 4.79e-174 | 499 |
| MS.gene32208.t1 | AT1G34575 | 54.604 | 467 | 200 | 4 | 3 | 466 | 65 | 522 | 3.39e-172 | 494 |
| MS.gene32208.t1 | AT1G30730 | 52.564 | 468 | 217 | 3 | 2 | 468 | 62 | 525 | 2.09e-171 | 493 |
| MS.gene32208.t1 | AT1G26400 | 53.419 | 468 | 212 | 6 | 4 | 470 | 65 | 527 | 5.25e-170 | 489 |
| MS.gene32208.t1 | AT1G30710 | 54.237 | 472 | 201 | 7 | 3 | 469 | 68 | 529 | 5.12e-169 | 487 |
| MS.gene32208.t1 | AT1G26410 | 54.957 | 464 | 203 | 5 | 4 | 466 | 89 | 547 | 9.97e-169 | 487 |
| MS.gene32208.t1 | AT1G30700 | 52.137 | 468 | 215 | 5 | 3 | 466 | 62 | 524 | 1.96e-163 | 472 |
| MS.gene32208.t1 | AT5G44400 | 49.679 | 467 | 227 | 6 | 4 | 466 | 72 | 534 | 1.45e-157 | 457 |
| MS.gene32208.t1 | AT5G44390 | 50.320 | 469 | 225 | 5 | 4 | 467 | 74 | 539 | 2.19e-157 | 457 |
| MS.gene32208.t1 | AT2G34810 | 49.574 | 470 | 231 | 3 | 3 | 467 | 70 | 538 | 7.02e-156 | 454 |
| MS.gene32208.t1 | AT2G34790 | 49.573 | 468 | 226 | 5 | 4 | 468 | 70 | 530 | 1.06e-155 | 453 |
| MS.gene32208.t1 | AT4G20840 | 50.851 | 470 | 218 | 7 | 3 | 467 | 73 | 534 | 7.76e-155 | 451 |
| MS.gene32208.t1 | AT1G30760 | 49.573 | 468 | 224 | 6 | 4 | 467 | 90 | 549 | 7.84e-153 | 446 |
| MS.gene32208.t1 | AT1G30760 | 49.573 | 468 | 224 | 6 | 4 | 467 | 72 | 531 | 8.59e-153 | 446 |
| MS.gene32208.t1 | AT1G11770 | 49.041 | 469 | 228 | 6 | 3 | 467 | 67 | 528 | 9.91e-150 | 437 |
| MS.gene32208.t1 | AT1G01980 | 50.947 | 475 | 215 | 9 | 3 | 467 | 67 | 533 | 3.19e-147 | 431 |
| MS.gene32208.t1 | AT5G44380 | 48.927 | 466 | 232 | 4 | 4 | 466 | 75 | 537 | 3.48e-147 | 431 |
| MS.gene32208.t1 | AT5G44380 | 48.927 | 466 | 232 | 4 | 4 | 466 | 115 | 577 | 9.84e-147 | 432 |
| MS.gene32208.t1 | AT4G20860 | 48.026 | 456 | 231 | 4 | 12 | 466 | 77 | 527 | 2.53e-145 | 426 |
| MS.gene32208.t1 | AT4G20820 | 47.854 | 466 | 229 | 5 | 3 | 466 | 71 | 524 | 2.58e-145 | 426 |
| MS.gene32208.t1 | AT5G44360 | 48.180 | 467 | 230 | 7 | 4 | 466 | 70 | 528 | 1.33e-140 | 414 |
| MS.gene32208.t1 | AT5G44360 | 48.180 | 467 | 230 | 7 | 4 | 466 | 86 | 544 | 1.48e-140 | 415 |
| MS.gene32208.t1 | AT4G20830 | 49.362 | 470 | 225 | 7 | 3 | 467 | 74 | 535 | 3.55e-140 | 413 |
| MS.gene32208.t1 | AT4G20830 | 49.362 | 470 | 225 | 7 | 3 | 467 | 74 | 535 | 1.30e-139 | 413 |
| MS.gene32208.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 65 | 527 | 1.95e-138 | 409 |
| MS.gene32208.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 135 | 597 | 3.08e-137 | 408 |
| MS.gene32208.t1 | AT5G44410 | 48.620 | 471 | 223 | 9 | 3 | 466 | 69 | 527 | 2.24e-135 | 401 |
| MS.gene32208.t1 | AT1G30740 | 47.447 | 470 | 236 | 7 | 3 | 467 | 64 | 527 | 5.47e-130 | 387 |
| MS.gene32208.t1 | AT1G30740 | 47.447 | 470 | 236 | 7 | 3 | 467 | 67 | 530 | 7.46e-130 | 387 |
Find 86 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCTAATGTTGTTCAAAT+TGG | 0.228780 | 4.4:-22689942 | MS.gene32208:CDS |
| AAGCTGAATGTTGTTGCTTT+TGG | 0.230709 | 4.4:+22690514 | None:intergenic |
| GGAAATTTGATGAGAAAATT+TGG | 0.258916 | 4.4:-22690242 | MS.gene32208:CDS |
| TTTGACATTGCCAAAACTTA+TGG | 0.263954 | 4.4:-22689336 | MS.gene32208:CDS |
| TGGCACATTTAACTGTTTCT+TGG | 0.267359 | 4.4:+22690534 | None:intergenic |
| AAACTTGCTTCCATAAGTTT+TGG | 0.271849 | 4.4:+22689326 | None:intergenic |
| AATAGATCTTTATGCAGTTT+TGG | 0.273624 | 4.4:+22689975 | None:intergenic |
| AGTAAGGAATTCCCCGAATT+AGG | 0.285169 | 4.4:-22689840 | MS.gene32208:CDS |
| TCCTTCATCTTTCCACCATA+TGG | 0.288030 | 4.4:+22689588 | None:intergenic |
| AACAAGCTTGCTTTCCCATC+TGG | 0.299204 | 4.4:-22690314 | MS.gene32208:CDS |
| GGGAGGCATTTCTCAATTAT+AGG | 0.301839 | 4.4:-22689401 | MS.gene32208:CDS |
| ACTTTGGTTCAAACCTAATT+CGG | 0.311377 | 4.4:+22689827 | None:intergenic |
| TGACCAATTTGAACAACATT+AGG | 0.312133 | 4.4:+22689939 | None:intergenic |
| ATAGATTCTAGAGCTTCTTT+AGG | 0.318699 | 4.4:+22689657 | None:intergenic |
| TCTAGAGCTTCTTTAGGAAT+AGG | 0.321024 | 4.4:+22689663 | None:intergenic |
| GTTATAATTGCCAAAGGTTT+CGG | 0.327951 | 4.4:+22690578 | None:intergenic |
| GGTGGTGGTGGTGCTAGTTT+TGG | 0.331271 | 4.4:-22690119 | MS.gene32208:CDS |
| AAGAAGCTCTAGAATCTATA+TGG | 0.334429 | 4.4:-22689653 | MS.gene32208:CDS |
| TCAAATCTCGTTACGTTACT+TGG | 0.342382 | 4.4:+22689354 | None:intergenic |
| CTTTGGTTCAAACCTAATTC+GGG | 0.343696 | 4.4:+22689828 | None:intergenic |
| CATTTCTCTGGTGGTGGGTA+TGG | 0.360817 | 4.4:-22690263 | MS.gene32208:CDS |
| AATTATAGGGATGCTGATAT+TGG | 0.376157 | 4.4:-22689387 | MS.gene32208:CDS |
| GTAGTGCAAGTTAGTTTCAT+TGG | 0.379275 | 4.4:-22689903 | MS.gene32208:CDS |
| GTGATTGTCTTTCAATGCCT+TGG | 0.383003 | 4.4:-22689806 | MS.gene32208:CDS |
| GGAGGCATTTCTCAATTATA+GGG | 0.389544 | 4.4:-22689400 | MS.gene32208:CDS |
| ACGATAACCTGTGGAGTTAT+CGG | 0.397449 | 4.4:+22690065 | None:intergenic |
| TAAATTGGTTGAGGATCCTT+TGG | 0.399190 | 4.4:+22689717 | None:intergenic |
| TACTCCTCTTGAAGCATTGT+TGG | 0.402290 | 4.4:-22689745 | MS.gene32208:CDS |
| TTCATATCTAAAGAAATTCT+CGG | 0.418836 | 4.4:+22689254 | None:intergenic |
| GAAATTCTCGGGATCAACTT+TGG | 0.424393 | 4.4:+22689266 | None:intergenic |
| GGTGGTCATTTCTCTGGTGG+TGG | 0.426644 | 4.4:-22690269 | MS.gene32208:CDS |
| TCAACAGCATGGGTTGAATC+AGG | 0.427451 | 4.4:-22690380 | MS.gene32208:CDS |
| ACAAGCTTGCTTTCCCATCT+GGG | 0.436569 | 4.4:-22690313 | MS.gene32208:CDS |
| TCTTTCATGGAAACTTCAAT+TGG | 0.441584 | 4.4:-22690090 | MS.gene32208:CDS |
| CTTATGCAATGGAATCCATA+TGG | 0.450449 | 4.4:-22689603 | MS.gene32208:CDS |
| TATTCTTGATAGAAAATCAA+TGG | 0.458066 | 4.4:-22690168 | MS.gene32208:CDS |
| GGTGCTGGTGGTCATTTCTC+TGG | 0.459504 | 4.4:-22690275 | MS.gene32208:CDS |
| TCTGGGGTCTGCTTTACTCT+AGG | 0.464774 | 4.4:-22690296 | MS.gene32208:CDS |
| AGCAACAACACCGAAACCTT+TGG | 0.466175 | 4.4:-22690588 | MS.gene32208:CDS |
| CTCGGAGAATTCGAAACATA+AGG | 0.468696 | 4.4:+22689423 | None:intergenic |
| GTCTGCTTTACTCTAGGTGC+TGG | 0.472600 | 4.4:-22690290 | MS.gene32208:CDS |
| CATATGGATTCCATTGCATA+AGG | 0.477148 | 4.4:+22689604 | None:intergenic |
| CAACTGATGTCGTTTACAAA+TGG | 0.490862 | 4.4:-22690010 | MS.gene32208:CDS |
| AGTGGCGGTCACGACTTTGA+AGG | 0.494173 | 4.4:-22690482 | MS.gene32208:CDS |
| GTGGTCATTTCTCTGGTGGT+GGG | 0.501321 | 4.4:-22690268 | MS.gene32208:CDS |
| AGAGTAAAGCAGACCCCAGA+TGG | 0.503056 | 4.4:+22690299 | None:intergenic |
| GACTTATACAATGATCTAGT+AGG | 0.504206 | 4.4:+22689222 | None:intergenic |
| AACAAGTCAAGTATAACAAA+AGG | 0.504620 | 4.4:+22690437 | None:intergenic |
| AACAAGTTTCCTACTCTATG+AGG | 0.510558 | 4.4:+22689540 | None:intergenic |
| TGCTTTACTCTAGGTGCTGG+TGG | 0.512366 | 4.4:-22690287 | MS.gene32208:CDS |
| TCATATCTAAAGAAATTCTC+GGG | 0.512401 | 4.4:+22689255 | None:intergenic |
| TGGGCTATAAGAGGTGGTGG+TGG | 0.518497 | 4.4:-22690131 | MS.gene32208:CDS |
| TTTCCTACTCTATGAGGAAA+CGG | 0.519543 | 4.4:+22689546 | None:intergenic |
| GCTTCAAGAGGAGTACCAAT+TGG | 0.520632 | 4.4:+22689753 | None:intergenic |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 4.4:-22690167 | MS.gene32208:CDS |
| GTGAGAATTTCCTTATGCAA+TGG | 0.526102 | 4.4:-22689614 | MS.gene32208:CDS |
| TCCATATGGTGGAAAGATGA+AGG | 0.526227 | 4.4:-22689589 | MS.gene32208:CDS |
| GTTGTTCAAATTGGTCAAGA+GGG | 0.530168 | 4.4:-22689933 | MS.gene32208:CDS |
| ATTGGTTCCGATAACTCCAC+AGG | 0.531147 | 4.4:-22690072 | MS.gene32208:CDS |
| ATATGGAAATTGATGATTGA+AGG | 0.537491 | 4.4:-22689636 | MS.gene32208:CDS |
| TTTACAAGAATCAACAGCAT+GGG | 0.543499 | 4.4:-22690390 | MS.gene32208:CDS |
| GCATTGAAAGACAATCACTT+TGG | 0.543501 | 4.4:+22689811 | None:intergenic |
| TGATTCAATACTTTAATCCG+TGG | 0.548605 | 4.4:-22689515 | MS.gene32208:CDS |
| TGTTGTTCAAATTGGTCAAG+AGG | 0.558808 | 4.4:-22689934 | MS.gene32208:CDS |
| AGCATTGTTGGAAGAACCAA+AGG | 0.559301 | 4.4:-22689733 | MS.gene32208:CDS |
| GCTGGTGGTCATTTCTCTGG+TGG | 0.564457 | 4.4:-22690272 | MS.gene32208:CDS |
| CAAGCTTGCTTTCCCATCTG+GGG | 0.567506 | 4.4:-22690312 | MS.gene32208:CDS |
| TTTGCAGTTATAATTGCCAA+AGG | 0.570639 | 4.4:+22690572 | None:intergenic |
| TGAGGAAACGGTGTTTCTGA+TGG | 0.577426 | 4.4:+22689558 | None:intergenic |
| GTTGAATCAGGTGCAACACT+TGG | 0.578127 | 4.4:-22690368 | MS.gene32208:CDS |
| TAATTGAGAAATGCCTCCCT+CGG | 0.594640 | 4.4:+22689405 | None:intergenic |
| TCTTCCAACAATGCTTCAAG+AGG | 0.594711 | 4.4:+22689741 | None:intergenic |
| ACACCGTTTCCTCATAGAGT+AGG | 0.600052 | 4.4:-22689549 | MS.gene32208:CDS |
| AAAAGAGTGGAATTAATCCA+AGG | 0.600902 | 4.4:+22689789 | None:intergenic |
| ATTTACAAGAATCAACAGCA+TGG | 0.603350 | 4.4:-22690391 | MS.gene32208:CDS |
| TTATGTTTCGAATTCTCCGA+GGG | 0.609571 | 4.4:-22689421 | MS.gene32208:CDS |
| TGTTTCGAATTCTCCGAGGG+AGG | 0.634404 | 4.4:-22689418 | MS.gene32208:CDS |
| GAGTAAAGCAGACCCCAGAT+GGG | 0.637967 | 4.4:+22690300 | None:intergenic |
| CAGCTTAGAATTCGAAGTGG+CGG | 0.641139 | 4.4:-22690497 | MS.gene32208:CDS |
| TCAGAAGAATCATTTGACCA+CGG | 0.643223 | 4.4:+22689498 | None:intergenic |
| TTTGGTTCAAACCTAATTCG+GGG | 0.645523 | 4.4:+22689829 | None:intergenic |
| ATGCAATGGAATCCATATGG+TGG | 0.646403 | 4.4:-22689600 | MS.gene32208:CDS |
| ACATTGAAAACGATAACCTG+TGG | 0.652393 | 4.4:+22690056 | None:intergenic |
| CTTATGTTTCGAATTCTCCG+AGG | 0.672859 | 4.4:-22689422 | MS.gene32208:CDS |
| GTGAAAAGGAATGTCAGTGA+AGG | 0.694179 | 4.4:-22690035 | MS.gene32208:CDS |
| ATTCAGCTTAGAATTCGAAG+TGG | 0.701549 | 4.4:-22690500 | MS.gene32208:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTATTTTTTTGATAAAT+TGG | + | chr4.4:22690133-22690152 | None:intergenic | 10.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | - | chr4.4:22689665-22689684 | MS.gene32208:CDS | 20.0% |
| !! | TATTCTTGATAGAAAATCAA+TGG | - | chr4.4:22689664-22689683 | MS.gene32208:CDS | 20.0% |
| !! | TTCATATCTAAAGAAATTCT+CGG | + | chr4.4:22690581-22690600 | None:intergenic | 20.0% |
| !!! | ACTTTTAATTTTGATGAGTA+AGG | - | chr4.4:22689976-22689995 | MS.gene32208:CDS | 20.0% |
| !!! | TTTTTTTGATAAATTGGTTG+AGG | + | chr4.4:22690127-22690146 | None:intergenic | 20.0% |
| ! | AACAAGTCAAGTATAACAAA+AGG | + | chr4.4:22689398-22689417 | None:intergenic | 25.0% |
| ! | GCAAAAATTGTTGATGTTAA+GGG | - | chr4.4:22689638-22689657 | MS.gene32208:CDS | 25.0% |
| ! | GGAAATTTGATGAGAAAATT+TGG | - | chr4.4:22689590-22689609 | MS.gene32208:CDS | 25.0% |
| ! | TCATATCTAAAGAAATTCTC+GGG | + | chr4.4:22690580-22690599 | None:intergenic | 25.0% |
| ! | TGCAAAAATTGTTGATGTTA+AGG | - | chr4.4:22689637-22689656 | MS.gene32208:CDS | 25.0% |
| !! | AATAGATCTTTATGCAGTTT+TGG | + | chr4.4:22689860-22689879 | None:intergenic | 25.0% |
| !! | AGTTTCATTGGTCAATTTTT+AGG | - | chr4.4:22689941-22689960 | MS.gene32208:CDS | 25.0% |
| !! | ATATGGAAATTGATGATTGA+AGG | - | chr4.4:22690196-22690215 | MS.gene32208:CDS | 25.0% |
| !! | TTATCGTTTTCAATGTGAAA+AGG | - | chr4.4:22689783-22689802 | MS.gene32208:CDS | 25.0% |
| AAAAGAGTGGAATTAATCCA+AGG | + | chr4.4:22690046-22690065 | None:intergenic | 30.0% | |
| AATTATAGGGATGCTGATAT+TGG | - | chr4.4:22690445-22690464 | MS.gene32208:CDS | 30.0% | |
| ATTTACAAGAATCAACAGCA+TGG | - | chr4.4:22689441-22689460 | MS.gene32208:CDS | 30.0% | |
| CAACCTAATGTTGTTCAAAT+TGG | - | chr4.4:22689890-22689909 | MS.gene32208:CDS | 30.0% | |
| GACTTATACAATGATCTAGT+AGG | + | chr4.4:22690613-22690632 | None:intergenic | 30.0% | |
| GTTATAATTGCCAAAGGTTT+CGG | + | chr4.4:22689257-22689276 | None:intergenic | 30.0% | |
| TCTTTCATGGAAACTTCAAT+TGG | - | chr4.4:22689742-22689761 | MS.gene32208:CDS | 30.0% | |
| TGACCAATTTGAACAACATT+AGG | + | chr4.4:22689896-22689915 | None:intergenic | 30.0% | |
| TGATTCAATACTTTAATCCG+TGG | - | chr4.4:22690317-22690336 | MS.gene32208:CDS | 30.0% | |
| TTTACAAGAATCAACAGCAT+GGG | - | chr4.4:22689442-22689461 | MS.gene32208:CDS | 30.0% | |
| TTTGACATTGCCAAAACTTA+TGG | - | chr4.4:22690496-22690515 | MS.gene32208:CDS | 30.0% | |
| TTTGCAGTTATAATTGCCAA+AGG | + | chr4.4:22689263-22689282 | None:intergenic | 30.0% | |
| ! | AACGTCATGTGAATTTTTCA+AGG | - | chr4.4:22690365-22690384 | MS.gene32208:CDS | 30.0% |
| ! | ACTTTGGTTCAAACCTAATT+CGG | + | chr4.4:22690008-22690027 | None:intergenic | 30.0% |
| !! | AAACTTGCTTCCATAAGTTT+TGG | + | chr4.4:22690509-22690528 | None:intergenic | 30.0% |
| !! | AAGAAGCTCTAGAATCTATA+TGG | - | chr4.4:22690179-22690198 | MS.gene32208:CDS | 30.0% |
| !! | ATAGATTCTAGAGCTTCTTT+AGG | + | chr4.4:22690178-22690197 | None:intergenic | 30.0% |
| !! | TATGGAAGCAAGTTTTTCAA+AGG | - | chr4.4:22690514-22690533 | MS.gene32208:CDS | 30.0% |
| !!! | AATGGGAGAAGATCTTTTTT+GGG | - | chr4.4:22689682-22689701 | MS.gene32208:CDS | 30.0% |
| !!! | AATTCCACTCTTTTTTGGTA+TGG | - | chr4.4:22690052-22690071 | MS.gene32208:CDS | 30.0% |
| !!! | GGATTAATTCCACTCTTTTT+TGG | - | chr4.4:22690047-22690066 | MS.gene32208:CDS | 30.0% |
| !!! | GTTTTGGTGTTATTCTTTCA+TGG | - | chr4.4:22689729-22689748 | MS.gene32208:CDS | 30.0% |
| AACAAGTTTCCTACTCTATG+AGG | + | chr4.4:22690295-22690314 | None:intergenic | 35.0% | |
| AATTGGTCAAGAGGGAAAAA+AGG | - | chr4.4:22689907-22689926 | MS.gene32208:CDS | 35.0% | |
| ACATTGAAAACGATAACCTG+TGG | + | chr4.4:22689779-22689798 | None:intergenic | 35.0% | |
| ATTCAGCTTAGAATTCGAAG+TGG | - | chr4.4:22689332-22689351 | MS.gene32208:CDS | 35.0% | |
| CATATGGATTCCATTGCATA+AGG | + | chr4.4:22690231-22690250 | None:intergenic | 35.0% | |
| CTTATGCAATGGAATCCATA+TGG | - | chr4.4:22690229-22690248 | MS.gene32208:CDS | 35.0% | |
| GCATTGAAAGACAATCACTT+TGG | + | chr4.4:22690024-22690043 | None:intergenic | 35.0% | |
| GTGAGAATTTCCTTATGCAA+TGG | - | chr4.4:22690218-22690237 | MS.gene32208:CDS | 35.0% | |
| GTTGTTCAAATTGGTCAAGA+GGG | - | chr4.4:22689899-22689918 | MS.gene32208:CDS | 35.0% | |
| TAAATTGGTTGAGGATCCTT+TGG | + | chr4.4:22690118-22690137 | None:intergenic | 35.0% | |
| TCAAATCTCGTTACGTTACT+TGG | + | chr4.4:22690481-22690500 | None:intergenic | 35.0% | |
| TCAGAAGAATCATTTGACCA+CGG | + | chr4.4:22690337-22690356 | None:intergenic | 35.0% | |
| TGTTGTTCAAATTGGTCAAG+AGG | - | chr4.4:22689898-22689917 | MS.gene32208:CDS | 35.0% | |
| TTATGTTTCGAATTCTCCGA+GGG | - | chr4.4:22690411-22690430 | MS.gene32208:CDS | 35.0% | |
| TTTCCTACTCTATGAGGAAA+CGG | + | chr4.4:22690289-22690308 | None:intergenic | 35.0% | |
| ! | CAACTGATGTCGTTTACAAA+TGG | - | chr4.4:22689822-22689841 | MS.gene32208:CDS | 35.0% |
| ! | CTTTGGTTCAAACCTAATTC+GGG | + | chr4.4:22690007-22690026 | None:intergenic | 35.0% |
| ! | GTAGTGCAAGTTAGTTTCAT+TGG | - | chr4.4:22689929-22689948 | MS.gene32208:CDS | 35.0% |
| ! | TCTAGAGCTTCTTTAGGAAT+AGG | + | chr4.4:22690172-22690191 | None:intergenic | 35.0% |
| ! | TGGCACATTTAACTGTTTCT+TGG | + | chr4.4:22689301-22689320 | None:intergenic | 35.0% |
| ! | TTTGGTTCAAACCTAATTCG+GGG | + | chr4.4:22690006-22690025 | None:intergenic | 35.0% |
| !! | GGAGGCATTTCTCAATTATA+GGG | - | chr4.4:22690432-22690451 | MS.gene32208:CDS | 35.0% |
| !!! | AAGCTGAATGTTGTTGCTTT+TGG | + | chr4.4:22689321-22689340 | None:intergenic | 35.0% |
| !!! | CAATGGGAGAAGATCTTTTT+TGG | - | chr4.4:22689681-22689700 | MS.gene32208:CDS | 35.0% |
| !!! | CTTGTTGCTTTTCTTTGCAA+TGG | + | chr4.4:22689504-22689523 | None:intergenic | 35.0% |
| !!! | GATCTTTTTTGGGCTATAAG+AGG | - | chr4.4:22689692-22689711 | MS.gene32208:CDS | 35.0% |
| ACGATAACCTGTGGAGTTAT+CGG | + | chr4.4:22689770-22689789 | None:intergenic | 40.0% | |
| AGCATTGTTGGAAGAACCAA+AGG | - | chr4.4:22690099-22690118 | MS.gene32208:CDS | 40.0% | |
| AGTAAGGAATTCCCCGAATT+AGG | - | chr4.4:22689992-22690011 | MS.gene32208:CDS | 40.0% | |
| ATGCAATGGAATCCATATGG+TGG | - | chr4.4:22690232-22690251 | MS.gene32208:CDS | 40.0% | |
| CTCGGAGAATTCGAAACATA+AGG | + | chr4.4:22690412-22690431 | None:intergenic | 40.0% | |
| CTGACCATACCAAAAAAGAG+TGG | + | chr4.4:22690059-22690078 | None:intergenic | 40.0% | |
| CTTATGTTTCGAATTCTCCG+AGG | - | chr4.4:22690410-22690429 | MS.gene32208:CDS | 40.0% | |
| GAAATTCTCGGGATCAACTT+TGG | + | chr4.4:22690569-22690588 | None:intergenic | 40.0% | |
| GTGAAAAGGAATGTCAGTGA+AGG | - | chr4.4:22689797-22689816 | MS.gene32208:CDS | 40.0% | |
| GTGATTGTCTTTCAATGCCT+TGG | - | chr4.4:22690026-22690045 | MS.gene32208:CDS | 40.0% | |
| TAATTGAGAAATGCCTCCCT+CGG | + | chr4.4:22690430-22690449 | None:intergenic | 40.0% | |
| TCCTTCATCTTTCCACCATA+TGG | + | chr4.4:22690247-22690266 | None:intergenic | 40.0% | |
| TCTTCCAACAATGCTTCAAG+AGG | + | chr4.4:22690094-22690113 | None:intergenic | 40.0% | |
| ! | TCCATATGGTGGAAAGATGA+AGG | - | chr4.4:22690243-22690262 | MS.gene32208:CDS | 40.0% |
| ! | TTTTGGTATGGTCAGCCAAT+TGG | - | chr4.4:22690064-22690083 | MS.gene32208:CDS | 40.0% |
| !! | GGGAGGCATTTCTCAATTAT+AGG | - | chr4.4:22690431-22690450 | MS.gene32208:CDS | 40.0% |
| !! | TACTCCTCTTGAAGCATTGT+TGG | - | chr4.4:22690087-22690106 | MS.gene32208:CDS | 40.0% |
| !!! | CTTTTTTGGGCTATAAGAGG+TGG | - | chr4.4:22689695-22689714 | MS.gene32208:CDS | 40.0% |
| ACACCGTTTCCTCATAGAGT+AGG | - | chr4.4:22690283-22690302 | MS.gene32208:CDS | 45.0% | |
| AGCAACAACACCGAAACCTT+TGG | - | chr4.4:22689244-22689263 | MS.gene32208:CDS | 45.0% | |
| CAGCTTAGAATTCGAAGTGG+CGG | - | chr4.4:22689335-22689354 | MS.gene32208:CDS | 45.0% | |
| GTTGAATCAGGTGCAACACT+TGG | - | chr4.4:22689464-22689483 | MS.gene32208:CDS | 45.0% | |
| ! | AACAAGCTTGCTTTCCCATC+TGG | - | chr4.4:22689518-22689537 | MS.gene32208:CDS | 45.0% |
| ! | ACAAGCTTGCTTTCCCATCT+GGG | - | chr4.4:22689519-22689538 | MS.gene32208:CDS | 45.0% |
| ! | ATTGGTTCCGATAACTCCAC+AGG | - | chr4.4:22689760-22689779 | MS.gene32208:CDS | 45.0% |
| ! | TCAACAGCATGGGTTGAATC+AGG | - | chr4.4:22689452-22689471 | MS.gene32208:CDS | 45.0% |
| ! | TGAGGAAACGGTGTTTCTGA+TGG | + | chr4.4:22690277-22690296 | None:intergenic | 45.0% |
| ! | TTTTGGGCTATAAGAGGTGG+TGG | - | chr4.4:22689698-22689717 | MS.gene32208:CDS | 45.0% |
| !! | GCTTCAAGAGGAGTACCAAT+TGG | + | chr4.4:22690082-22690101 | None:intergenic | 45.0% |
| AGAGTAAAGCAGACCCCAGA+TGG | + | chr4.4:22689536-22689555 | None:intergenic | 50.0% | |
| GAGTAAAGCAGACCCCAGAT+GGG | + | chr4.4:22689535-22689554 | None:intergenic | 50.0% | |
| GTCTGCTTTACTCTAGGTGC+TGG | - | chr4.4:22689542-22689561 | MS.gene32208:CDS | 50.0% | |
| GTGGTCATTTCTCTGGTGGT+GGG | - | chr4.4:22689564-22689583 | MS.gene32208:CDS | 50.0% | |
| TGTTTCGAATTCTCCGAGGG+AGG | - | chr4.4:22690414-22690433 | MS.gene32208:CDS | 50.0% | |
| ! | CAAGCTTGCTTTCCCATCTG+GGG | - | chr4.4:22689520-22689539 | MS.gene32208:CDS | 50.0% |
| ! | TCTGGGGTCTGCTTTACTCT+AGG | - | chr4.4:22689536-22689555 | MS.gene32208:CDS | 50.0% |
| !! | CATTTCTCTGGTGGTGGGTA+TGG | - | chr4.4:22689569-22689588 | MS.gene32208:CDS | 50.0% |
| !! | TGCTTTACTCTAGGTGCTGG+TGG | - | chr4.4:22689545-22689564 | MS.gene32208:CDS | 50.0% |
| AGTGGCGGTCACGACTTTGA+AGG | - | chr4.4:22689350-22689369 | MS.gene32208:CDS | 55.0% | |
| GCTGGTGGTCATTTCTCTGG+TGG | - | chr4.4:22689560-22689579 | MS.gene32208:CDS | 55.0% | |
| GGTGGTCATTTCTCTGGTGG+TGG | - | chr4.4:22689563-22689582 | MS.gene32208:CDS | 55.0% | |
| ! | TGGGCTATAAGAGGTGGTGG+TGG | - | chr4.4:22689701-22689720 | MS.gene32208:CDS | 55.0% |
| !! | GGTGCTGGTGGTCATTTCTC+TGG | - | chr4.4:22689557-22689576 | MS.gene32208:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGCTAGTTT+TGG | - | chr4.4:22689713-22689732 | MS.gene32208:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 22689215 | 22690639 | 22689215 | ID=MS.gene32208 |
| chr4.4 | mRNA | 22689215 | 22690639 | 22689215 | ID=MS.gene32208.t1;Parent=MS.gene32208 |
| chr4.4 | exon | 22689215 | 22690639 | 22689215 | ID=MS.gene32208.t1.exon1;Parent=MS.gene32208.t1 |
| chr4.4 | CDS | 22689215 | 22690639 | 22689215 | ID=cds.MS.gene32208.t1;Parent=MS.gene32208.t1 |
| Gene Sequence |
| Protein sequence |
