Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32601.t1 | XP_003603440.1 | 98.3 | 709 | 11 | 1 | 1 | 709 | 1 | 708 | 0.00E+00 | 1380.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32601.t1 | O78328 | 62.0 | 655 | 243 | 4 | 58 | 707 | 65 | 718 | 2.3e-229 | 796.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32601.t1 | G7JBM5 | 98.3 | 709 | 11 | 1 | 1 | 709 | 1 | 708 | 0.0e+00 | 1380.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052239 | MS.gene32601 | -0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene060134 | MS.gene32601 | 0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene32601 | 0.812948 | 3.28E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene32601 | 0.824845 | 6.47E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32601.t1 | MTR_3g107740 | 98.307 | 709 | 11 | 1 | 1 | 709 | 1 | 708 | 0.0 | 1436 |
| MS.gene32601.t1 | MTR_3g107740 | 96.684 | 573 | 18 | 1 | 1 | 573 | 1 | 572 | 0.0 | 1144 |
| MS.gene32601.t1 | MTR_4g118640 | 61.457 | 659 | 246 | 4 | 55 | 707 | 60 | 716 | 0.0 | 820 |
| MS.gene32601.t1 | MTR_2g020590 | 57.589 | 672 | 274 | 6 | 44 | 709 | 50 | 716 | 0.0 | 776 |
| MS.gene32601.t1 | MTR_4g118640 | 58.270 | 659 | 222 | 5 | 55 | 707 | 60 | 671 | 0.0 | 762 |
| MS.gene32601.t1 | MTR_4g118640 | 60.709 | 621 | 236 | 4 | 55 | 669 | 60 | 678 | 0.0 | 758 |
| MS.gene32601.t1 | MTR_8g068300 | 56.555 | 656 | 275 | 5 | 58 | 706 | 58 | 710 | 0.0 | 753 |
| MS.gene32601.t1 | MTR_8g068265 | 56.707 | 656 | 274 | 5 | 58 | 706 | 58 | 710 | 0.0 | 752 |
| MS.gene32601.t1 | MTR_7g005380 | 27.468 | 233 | 142 | 8 | 422 | 635 | 66 | 290 | 2.20e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32601.t1 | AT5G11380 | 68.398 | 693 | 192 | 5 | 19 | 707 | 30 | 699 | 0.0 | 959 |
| MS.gene32601.t1 | AT4G15560 | 59.639 | 664 | 257 | 5 | 49 | 704 | 56 | 716 | 0.0 | 797 |
| MS.gene32601.t1 | AT3G21500 | 55.224 | 670 | 260 | 5 | 41 | 704 | 39 | 674 | 0.0 | 733 |
| MS.gene32601.t1 | AT5G11380 | 64.632 | 557 | 170 | 5 | 19 | 571 | 30 | 563 | 0.0 | 723 |
| MS.gene32601.t1 | AT5G11380 | 65.209 | 549 | 164 | 5 | 19 | 563 | 30 | 555 | 0.0 | 720 |
| MS.gene32601.t1 | AT5G11380 | 65.844 | 527 | 153 | 5 | 19 | 541 | 30 | 533 | 0.0 | 692 |
| MS.gene32601.t1 | AT3G21500 | 54.167 | 624 | 247 | 5 | 41 | 659 | 39 | 628 | 0.0 | 667 |
| MS.gene32601.t1 | AT3G21500 | 54.080 | 625 | 247 | 5 | 41 | 659 | 39 | 629 | 0.0 | 667 |
| MS.gene32601.t1 | AT5G50850 | 29.130 | 230 | 142 | 8 | 422 | 635 | 74 | 298 | 5.74e-14 | 74.7 |
Find 172 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTTCGGAAAGATTTAC+TGG | 0.231822 | 3.1:+85259532 | None:intergenic |
| TGATTCTGGAAAGGGGAAAT+TGG | 0.233981 | 3.1:+85260583 | None:intergenic |
| GCCGTCACTTAAACTGTTTA+TGG | 0.240402 | 3.1:-85255505 | MS.gene32601:CDS |
| TCTTCAAGAAGTAGCTTCTT+TGG | 0.252475 | 3.1:-85256776 | MS.gene32601:CDS |
| TGCCACAGCATTGAGTTTGC+TGG | 0.261355 | 3.1:-85253433 | MS.gene32601:CDS |
| CACGCTGTCACATTTGCTTC+TGG | 0.266276 | 3.1:-85255438 | MS.gene32601:CDS |
| CATTCACTTCTTGCAAATCT+TGG | 0.276684 | 3.1:-85253906 | MS.gene32601:CDS |
| AGGTTACTTGGACTCAAATT+TGG | 0.278392 | 3.1:-85259657 | MS.gene32601:CDS |
| AGAAGAATACAAGGTGATTC+TGG | 0.295885 | 3.1:+85260569 | None:intergenic |
| GCTCAATTTATTGCTCTTGA+TGG | 0.301990 | 3.1:-85253750 | MS.gene32601:CDS |
| GGTAAAGATGTTGCCTTATT+GGG | 0.306782 | 3.1:-85253963 | MS.gene32601:CDS |
| GAAGGGTCTATTGGAGGATT+TGG | 0.309133 | 3.1:-85253783 | MS.gene32601:CDS |
| GGTGCCATGACAATCATATT+TGG | 0.314187 | 3.1:+85254322 | None:intergenic |
| GTCATAGGCTCTCTGTAAAA+AGG | 0.315685 | 3.1:+85255371 | None:intergenic |
| GTCTTTGTCTTTCTCAGCTT+CGG | 0.321151 | 3.1:+85255560 | None:intergenic |
| TAGCAGCTTCTCTAAACTTT+CGG | 0.328548 | 3.1:+85259519 | None:intergenic |
| AGGTAAAGATGTTGCCTTAT+TGG | 0.329622 | 3.1:-85253964 | MS.gene32601:CDS |
| AGCAATCCAATCTGCGAGTT+AGG | 0.330179 | 3.1:+85260613 | None:intergenic |
| ACTTCTCGACCGAAGAATTA+AGG | 0.337143 | 3.1:-85253727 | MS.gene32601:intron |
| CTACTCTATTTGAAGAGCTT+GGG | 0.339586 | 3.1:-85256852 | MS.gene32601:CDS |
| AATATTACTGAATCCTAGTA+TGG | 0.348105 | 3.1:-85260355 | MS.gene32601:CDS |
| CGTTGGTCAATTACCTCAAT+TGG | 0.351062 | 3.1:+85254175 | None:intergenic |
| CCAATCTGCGAGTTAGGGTT+TGG | 0.351819 | 3.1:+85260619 | None:intergenic |
| TGTTATAACTGATGAAAATC+AGG | 0.353490 | 3.1:-85256728 | MS.gene32601:CDS |
| GGATTCAATGGGTCCTGTCT+TGG | 0.353501 | 3.1:-85256755 | MS.gene32601:CDS |
| TATCCTAGGGGTGCCTTGGT+TGG | 0.359144 | 3.1:-85254226 | MS.gene32601:CDS |
| CAGTTTGCTTTCGGTATCCT+AGG | 0.359222 | 3.1:-85254240 | MS.gene32601:CDS |
| CATTGATCCATATCCCAATA+AGG | 0.366694 | 3.1:+85253950 | None:intergenic |
| GCAGTTGCAAGGGATATTAA+AGG | 0.367112 | 3.1:-85259758 | MS.gene32601:CDS |
| TATGCACATAAGATACTTAC+AGG | 0.367465 | 3.1:-85260120 | MS.gene32601:CDS |
| GATGTTGCCTTATTGGGATA+TGG | 0.373978 | 3.1:-85253957 | MS.gene32601:CDS |
| AGTAGCTTCTTTGGATTCAA+TGG | 0.378602 | 3.1:-85256767 | MS.gene32601:CDS |
| CACAATTCACAAACACATAA+AGG | 0.378697 | 3.1:+85253389 | None:intergenic |
| TCGATATTGTGTCCATCCAC+TGG | 0.382005 | 3.1:+85256814 | None:intergenic |
| AACTAATTACCTTAATTCTT+CGG | 0.383912 | 3.1:+85253718 | None:intergenic |
| ACCGTTGAGGAAGGGTCTAT+TGG | 0.384406 | 3.1:-85253792 | MS.gene32601:CDS |
| ATGATCAACCAGTTTGCTTT+CGG | 0.387179 | 3.1:-85254249 | MS.gene32601:CDS |
| GAAGAAGGTATAATGCAAAA+TGG | 0.389504 | 3.1:+85255393 | None:intergenic |
| GCTTGGTTAAGCTGTTGATT+TGG | 0.391371 | 3.1:+85253480 | None:intergenic |
| CTTGAAGAACCTTTCTCAGC+AGG | 0.397023 | 3.1:-85260439 | MS.gene32601:CDS |
| GATTCTGGAAAGGGGAAATT+GGG | 0.398441 | 3.1:+85260584 | None:intergenic |
| ATCCTAGGGGTGCCTTGGTT+GGG | 0.404456 | 3.1:-85254225 | MS.gene32601:CDS |
| TCTACTCTATTTGAAGAGCT+TGG | 0.406045 | 3.1:-85256853 | MS.gene32601:CDS |
| CCAAACCCTAACTCGCAGAT+TGG | 0.406186 | 3.1:-85260619 | MS.gene32601:CDS |
| CTGATATTGATGATTTCTAC+TGG | 0.411312 | 3.1:-85260509 | MS.gene32601:CDS |
| TCCCACAATATCTTGTCAAC+TGG | 0.413014 | 3.1:+85260288 | None:intergenic |
| ATAAGTATGAACCTGGTCAT+AGG | 0.419660 | 3.1:+85255356 | None:intergenic |
| GACATTGTGGTTGTTCATGC+AGG | 0.429229 | 3.1:-85255540 | MS.gene32601:CDS |
| TGATTGCTTTGTGGAGAGTT+TGG | 0.431702 | 3.1:-85255586 | MS.gene32601:CDS |
| GTTTCAACTCCTGCTGAGAA+AGG | 0.438184 | 3.1:+85260430 | None:intergenic |
| GAGAGTGAATACGATGCATT+TGG | 0.440095 | 3.1:-85260033 | MS.gene32601:CDS |
| AATGAGCAATGCAGGTTACT+TGG | 0.443326 | 3.1:-85259669 | MS.gene32601:CDS |
| TAACTGTCTGGTAAAACAAT+TGG | 0.443341 | 3.1:+85253519 | None:intergenic |
| CTTATTGGGATATGGATCAA+TGG | 0.444909 | 3.1:-85253949 | MS.gene32601:CDS |
| CTTAACCAAGCAGGGTTGAC+TGG | 0.445093 | 3.1:-85253468 | MS.gene32601:CDS |
| TCGGTATCCTAGGGGTGCCT+TGG | 0.446965 | 3.1:-85254230 | MS.gene32601:CDS |
| ATACAAGGTGATTCTGGAAA+GGG | 0.448453 | 3.1:+85260575 | None:intergenic |
| CAATCTGCGAGTTAGGGTTT+GGG | 0.451051 | 3.1:+85260620 | None:intergenic |
| TACTGGATTGGAGCCTGCTA+AGG | 0.451075 | 3.1:+85259549 | None:intergenic |
| GAATGGAAAGGGATCACAAA+TGG | 0.451871 | 3.1:+85260642 | None:intergenic |
| AGGTCCACCACAGCCATACT+AGG | 0.452040 | 3.1:+85260342 | None:intergenic |
| CAGTAGAAATCATCAATATC+AGG | 0.455874 | 3.1:+85260510 | None:intergenic |
| TTCGGAAAGATTTACTGGAT+TGG | 0.457275 | 3.1:+85259537 | None:intergenic |
| TCTTGCAAATCTTGGCATTG+AGG | 0.460171 | 3.1:-85253898 | MS.gene32601:CDS |
| TAGTTGCAGTTATCAGCAAT+TGG | 0.468844 | 3.1:-85259721 | MS.gene32601:CDS |
| GTAGCTTCTTTGGATTCAAT+GGG | 0.476127 | 3.1:-85256766 | MS.gene32601:CDS |
| GCAATCCAATCTGCGAGTTA+GGG | 0.477857 | 3.1:+85260614 | None:intergenic |
| AAATAGAAGACAACTCTAAA+CGG | 0.479369 | 3.1:+85260391 | None:intergenic |
| GCTGGTCGGCTTCAAACATA+TGG | 0.480453 | 3.1:-85255609 | MS.gene32601:CDS |
| GCCACAGCATTGAGTTTGCT+GGG | 0.483694 | 3.1:-85253432 | MS.gene32601:CDS |
| CTAGGATACCGAAAGCAAAC+TGG | 0.484097 | 3.1:+85254241 | None:intergenic |
| TTAGGGTTTGGGAATGGAAA+GGG | 0.485075 | 3.1:+85260631 | None:intergenic |
| AAGGGAAGAATTCTTGTTGA+AGG | 0.485127 | 3.1:-85253984 | MS.gene32601:CDS |
| AGTTTGCTTTCGGTATCCTA+GGG | 0.492401 | 3.1:-85254239 | MS.gene32601:CDS |
| TCTGATTTGTTAGACAATGC+TGG | 0.494059 | 3.1:-85255627 | MS.gene32601:CDS |
| TGTCATTACAAGTGCAGGAT+TGG | 0.494629 | 3.1:-85254404 | MS.gene32601:CDS |
| AGCAATTGGACAACTATGTC+TGG | 0.495383 | 3.1:-85259707 | MS.gene32601:CDS |
| GCCTCAGAAGCTCGATGTCA+AGG | 0.495419 | 3.1:+85253844 | None:intergenic |
| AACATATGGTGATTGCTTTG+TGG | 0.497898 | 3.1:-85255595 | MS.gene32601:CDS |
| AGGATAGAGCTTCACGAGTA+CGG | 0.499924 | 3.1:+85253409 | None:intergenic |
| CAGTTGCAAGGGATATTAAA+GGG | 0.501421 | 3.1:-85259757 | MS.gene32601:CDS |
| TGAATATGCTCGTGGTATGA+TGG | 0.502051 | 3.1:-85256887 | MS.gene32601:CDS |
| CAACGGTAATTAGAAAAGAA+TGG | 0.504355 | 3.1:+85253808 | None:intergenic |
| TTGAAGATTTGAGTAATGAT+GGG | 0.518387 | 3.1:-85260684 | None:intergenic |
| ATTCATACACAGATTGGAAA+GGG | 0.519161 | 3.1:-85254002 | MS.gene32601:intron |
| GGATGCAACAGTATATCTGC+TGG | 0.522802 | 3.1:-85260000 | MS.gene32601:CDS |
| TTCAGGAATGGCAGTTGCAA+GGG | 0.523513 | 3.1:-85259768 | MS.gene32601:intron |
| TTACTTGGACTCAAATTTGG+TGG | 0.523809 | 3.1:-85259654 | MS.gene32601:CDS |
| TGCGAGTTAGGGTTTGGGAA+TGG | 0.525876 | 3.1:+85260625 | None:intergenic |
| TTTCTAATTACCGTTGAGGA+AGG | 0.526295 | 3.1:-85253801 | MS.gene32601:CDS |
| CTCTATCTAGTACCCTTAGC+AGG | 0.526339 | 3.1:-85259562 | MS.gene32601:CDS |
| TTGGACAACTATGTCTGGCC+AGG | 0.526907 | 3.1:-85259702 | MS.gene32601:CDS |
| AATGGAAAGGGATCACAAAT+GGG | 0.527413 | 3.1:+85260643 | None:intergenic |
| CGATATTGTGTCCATCCACT+GGG | 0.527659 | 3.1:+85256815 | None:intergenic |
| CGTGGTATGATGGGTCCACC+AGG | 0.528974 | 3.1:-85256877 | MS.gene32601:CDS |
| GATGCATTTGGTGCAGGTCA+TGG | 0.529726 | 3.1:-85260021 | MS.gene32601:CDS |
| CTCCAGTTGACAAGATATTG+TGG | 0.529849 | 3.1:-85260290 | MS.gene32601:CDS |
| ATTACAAGTGCAGGATTGGT+AGG | 0.531830 | 3.1:-85254400 | MS.gene32601:CDS |
| AAAGGTTCTTCAAGCAAAGA+GGG | 0.533111 | 3.1:+85260448 | None:intergenic |
| AATCAACAGCTTAACCAAGC+AGG | 0.533615 | 3.1:-85253477 | MS.gene32601:CDS |
| CCTCAGAAGCTCGATGTCAA+GGG | 0.536808 | 3.1:+85253845 | None:intergenic |
| TTTCAGGAATGGCAGTTGCA+AGG | 0.538184 | 3.1:-85259769 | MS.gene32601:intron |
| TATGAGGCAATGAGCAATGC+AGG | 0.539433 | 3.1:-85259677 | MS.gene32601:CDS |
| GTTATAACATGAACCAAGAC+AGG | 0.540223 | 3.1:+85256742 | None:intergenic |
| GCATGTTCAATATAACTGTC+TGG | 0.540461 | 3.1:+85253507 | None:intergenic |
| TCTAGAAGAAGAAGAATACA+AGG | 0.540511 | 3.1:+85260560 | None:intergenic |
| CAGGGTGTTACAAAACGTAT+TGG | 0.541232 | 3.1:-85256943 | MS.gene32601:CDS |
| TGCTCATTGCCTCATAGACC+TGG | 0.542521 | 3.1:+85259684 | None:intergenic |
| CCCTTGACATCGAGCTTCTG+AGG | 0.543920 | 3.1:-85253845 | MS.gene32601:CDS |
| AACAGTATATCTGCTGGACT+TGG | 0.544844 | 3.1:-85259994 | MS.gene32601:intron |
| AATGGTTCAGAACTGTCTAA+AGG | 0.546738 | 3.1:-85253931 | MS.gene32601:CDS |
| GTTGAGGAAGGGTCTATTGG+AGG | 0.546973 | 3.1:-85253789 | MS.gene32601:CDS |
| ATCAAATGCTCCACACTGGA+GGG | 0.547141 | 3.1:+85254366 | None:intergenic |
| GAATACGATGCATTTGGTGC+AGG | 0.548872 | 3.1:-85260027 | MS.gene32601:CDS |
| GAGCAATAAATTGAGCAACA+TGG | 0.551323 | 3.1:+85253757 | None:intergenic |
| AGCAATAAATTGAGCAACAT+GGG | 0.552047 | 3.1:+85253758 | None:intergenic |
| TCCATAAACAGTTTAAGTGA+CGG | 0.557677 | 3.1:+85255504 | None:intergenic |
| AAAGTTTAGAGAAGCTGCTA+AGG | 0.557892 | 3.1:-85259516 | MS.gene32601:intron |
| GCTTCTGGTTTATCATGTGG+TGG | 0.559580 | 3.1:-85255423 | MS.gene32601:CDS |
| TATTCATACACAGATTGGAA+AGG | 0.566531 | 3.1:-85254003 | MS.gene32601:intron |
| GCAGGATTGGTAGGTTCAGA+TGG | 0.569526 | 3.1:-85254391 | MS.gene32601:CDS |
| GAGCTTGGGTTGTATTACAT+AGG | 0.570421 | 3.1:-85256838 | MS.gene32601:CDS |
| GAATATGCTCGTGGTATGAT+GGG | 0.571731 | 3.1:-85256886 | MS.gene32601:CDS |
| TTCTAATTACCGTTGAGGAA+GGG | 0.573324 | 3.1:-85253800 | MS.gene32601:CDS |
| ATCAACAGCTTAACCAAGCA+GGG | 0.577584 | 3.1:-85253476 | MS.gene32601:CDS |
| ATACATAACTGACAAGCAGC+AGG | 0.578705 | 3.1:-85256683 | MS.gene32601:CDS |
| TGATATTGATGATTTCTACT+GGG | 0.582897 | 3.1:-85260508 | MS.gene32601:CDS |
| AATACAAGGTGATTCTGGAA+AGG | 0.583871 | 3.1:+85260574 | None:intergenic |
| CTTGTAATGACAAAACGCAC+TGG | 0.585950 | 3.1:+85254415 | None:intergenic |
| TCCAATAGACCCTTCCTCAA+CGG | 0.587245 | 3.1:+85253791 | None:intergenic |
| TTTGCTTCTGGTTTATCATG+TGG | 0.587877 | 3.1:-85255426 | MS.gene32601:CDS |
| ACTGGATTGGAGCCTGCTAA+GGG | 0.588415 | 3.1:+85259550 | None:intergenic |
| ACATGATGGAATTCCAATTG+AGG | 0.588705 | 3.1:-85254188 | MS.gene32601:intron |
| GAAAGGTTCTTCAAGCAAAG+AGG | 0.590345 | 3.1:+85260447 | None:intergenic |
| GTTAGGGTTTGGGAATGGAA+AGG | 0.592204 | 3.1:+85260630 | None:intergenic |
| AGGTGACTGTTGCTGATGCA+AGG | 0.592702 | 3.1:-85253878 | MS.gene32601:CDS |
| TATGTCTGGCCAGGTCTATG+AGG | 0.594144 | 3.1:-85259693 | MS.gene32601:CDS |
| ACAAGTTCAGCCTCATCAGA+TGG | 0.597230 | 3.1:+85254301 | None:intergenic |
| AAAGACGAAGCTATACATGA+TGG | 0.597974 | 3.1:-85254202 | MS.gene32601:CDS |
| ACTGAATCCTAGTATGGCTG+TGG | 0.598944 | 3.1:-85260349 | MS.gene32601:CDS |
| GAATCCTAGTATGGCTGTGG+TGG | 0.600379 | 3.1:-85260346 | MS.gene32601:CDS |
| TATTACATAGGCCCAGTGGA+TGG | 0.600676 | 3.1:-85256826 | MS.gene32601:CDS |
| ATTTGTTAGACAATGCTGGT+CGG | 0.603925 | 3.1:-85255623 | MS.gene32601:CDS |
| TACAAGGTGATTCTGGAAAG+GGG | 0.607701 | 3.1:+85260576 | None:intergenic |
| TATCAAATGCTCCACACTGG+AGG | 0.611958 | 3.1:+85254365 | None:intergenic |
| TTCCCAACCAAGGCACCCCT+AGG | 0.612053 | 3.1:+85254223 | None:intergenic |
| CTCAGAAGCTCGATGTCAAG+GGG | 0.612883 | 3.1:+85253846 | None:intergenic |
| GATGTCCAGTCAACCCTGCT+TGG | 0.614015 | 3.1:+85253463 | None:intergenic |
| TCCAGTTGACAAGATATTGT+GGG | 0.614631 | 3.1:-85260289 | MS.gene32601:CDS |
| TGAGGCTGAACTTGTGCACA+TGG | 0.615311 | 3.1:-85254293 | MS.gene32601:CDS |
| GTTACAAAACGTATTGGCAG+AGG | 0.616921 | 3.1:-85256937 | MS.gene32601:CDS |
| TCTTCAAATAGAGTAGAACC+TGG | 0.617072 | 3.1:+85256859 | None:intergenic |
| TCAAATGCTCCACACTGGAG+GGG | 0.618009 | 3.1:+85254367 | None:intergenic |
| CTTACCAAATATGATTGTCA+TGG | 0.622425 | 3.1:-85254326 | MS.gene32601:CDS |
| TCAGATGGTCCCCTCCAGTG+TGG | 0.622752 | 3.1:-85254376 | MS.gene32601:CDS |
| TGAGAAAGACAAAGACATTG+TGG | 0.627386 | 3.1:-85255553 | MS.gene32601:CDS |
| AGCTTCGTCTTTCCCAACCA+AGG | 0.630651 | 3.1:+85254213 | None:intergenic |
| TTTACAGAGAGCCTATGACC+AGG | 0.633765 | 3.1:-85255367 | MS.gene32601:intron |
| CACATAAGATACTTACAGGA+CGG | 0.634565 | 3.1:-85260116 | MS.gene32601:CDS |
| TTATATCAAATGCTCCACAC+TGG | 0.640253 | 3.1:+85254362 | None:intergenic |
| GCCCAGCAAACTCAATGCTG+TGG | 0.647181 | 3.1:+85253431 | None:intergenic |
| GACAAGATATTGTGGGATGT+CGG | 0.651340 | 3.1:-85260282 | MS.gene32601:CDS |
| GTTTGCTTTCGGTATCCTAG+GGG | 0.655988 | 3.1:-85254238 | MS.gene32601:CDS |
| TCAAATAGAGTAGAACCTGG+TGG | 0.656357 | 3.1:+85256862 | None:intergenic |
| ACTGTGTCAAGAATGGGTGT+TGG | 0.665774 | 3.1:+85260477 | None:intergenic |
| AAAGTTGATGAATATGCTCG+TGG | 0.672546 | 3.1:-85256895 | MS.gene32601:CDS |
| TGTCATGGCACCATCTGATG+AGG | 0.674548 | 3.1:-85254311 | MS.gene32601:CDS |
| ATAACTGACAAGCAGCAGGA+TGG | 0.691219 | 3.1:-85256679 | MS.gene32601:intron |
| GTTGTATTACATAGGCCCAG+TGG | 0.704282 | 3.1:-85256830 | MS.gene32601:CDS |
| ACTAGATAGAGCATTGACAG+AGG | 0.707471 | 3.1:+85259574 | None:intergenic |
| CATGATGTATTGCAACTGTG+AGG | 0.764353 | 3.1:+85260322 | None:intergenic |
| GGCTGAACTTGTGCACATGG+TGG | 0.765179 | 3.1:-85254290 | MS.gene32601:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 85253395 | 85260691 | 85253395 | ID=MS.gene32601 |
| chr3.1 | mRNA | 85253395 | 85260691 | 85253395 | ID=MS.gene32601.t1;Parent=MS.gene32601 |
| chr3.1 | exon | 85260275 | 85260691 | 85260275 | ID=MS.gene32601.t1.exon1;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85260275 | 85260691 | 85260275 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85259995 | 85260145 | 85259995 | ID=MS.gene32601.t1.exon2;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85259995 | 85260145 | 85259995 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85259517 | 85259785 | 85259517 | ID=MS.gene32601.t1.exon3;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85259517 | 85259785 | 85259517 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85256680 | 85256962 | 85256680 | ID=MS.gene32601.t1.exon4;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85256680 | 85256962 | 85256680 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85255368 | 85255660 | 85255368 | ID=MS.gene32601.t1.exon5;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85255368 | 85255660 | 85255368 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85254189 | 85254467 | 85254189 | ID=MS.gene32601.t1.exon6;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85254189 | 85254467 | 85254189 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85253728 | 85254012 | 85253728 | ID=MS.gene32601.t1.exon7;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85253728 | 85254012 | 85253728 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| chr3.1 | exon | 85253395 | 85253547 | 85253395 | ID=MS.gene32601.t1.exon8;Parent=MS.gene32601.t1 |
| chr3.1 | CDS | 85253395 | 85253547 | 85253395 | ID=cds.MS.gene32601.t1;Parent=MS.gene32601.t1 |
| Gene Sequence |
| Protein sequence |
