Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33332.t1 | KEH28745.1 | 76.9 | 39 | 9 | 0 | 18 | 56 | 45 | 83 | 1.60E-08 | 68.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33332.t1 | A0A072US92 | 76.9 | 39 | 9 | 0 | 18 | 56 | 45 | 83 | 1.2e-08 | 68.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene33332 | 0.800607 | 1.33E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene33332 | 0.815782 | 7.75E-52 | -1.69E-46 |
| MS.gene051201 | MS.gene33332 | 0.800455 | 1.43E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene33332 | 0.810467 | 1.14E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene33332 | 0.810605 | 1.06E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene33332 | 0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene33332 | 0.807822 | 4.19E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene33332 | 0.809779 | 1.60E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33332.t1 | MTR_4g011670 | 74.359 | 39 | 10 | 0 | 18 | 56 | 21 | 59 | 4.19e-16 | 65.1 |
| MS.gene33332.t1 | MTR_4g011650 | 76.923 | 39 | 9 | 0 | 18 | 56 | 45 | 83 | 4.47e-16 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTTACAGGTGGTTTATC+TGG | 0.203272 | 4.4:-87865666 | MS.gene33332:intron |
| CAAGAGAGGGAATATGAGTT+AGG | 0.317990 | 4.4:+87866630 | None:intergenic |
| TACAGGTGGTTTATCTGGAT+GGG | 0.395118 | 4.4:-87865661 | MS.gene33332:intron |
| GCGGAAGCACTTGGGTTAGA+TGG | 0.414835 | 4.4:-87865558 | MS.gene33332:CDS |
| TTACAGGTGGTTTATCTGGA+TGG | 0.416352 | 4.4:-87865662 | MS.gene33332:intron |
| ATGGTTGTTTATAACCAATC+TGG | 0.418762 | 4.4:-87865636 | MS.gene33332:CDS |
| CCAGTGTTGGCGGAAGCACT+TGG | 0.433243 | 4.4:-87865567 | MS.gene33332:CDS |
| CTAACTCATATTCCCTCTCT+TGG | 0.435280 | 4.4:-87866629 | MS.gene33332:CDS |
| ACAGGTGGTTTATCTGGATG+GGG | 0.467915 | 4.4:-87865660 | MS.gene33332:intron |
| AAGAGAGGGAATATGAGTTA+GGG | 0.477690 | 4.4:+87866631 | None:intergenic |
| CAACACTGGATCAATTTCAA+TGG | 0.485295 | 4.4:+87865581 | None:intergenic |
| AAAATTGCACCACACCAGAT+TGG | 0.488889 | 4.4:+87865622 | None:intergenic |
| CCAAGTGCTTCCGCCAACAC+TGG | 0.527594 | 4.4:+87865567 | None:intergenic |
| GGAAAGAATGTGCCTTGTCT+AGG | 0.529055 | 4.4:-87866608 | MS.gene33332:intron |
| GGCACATTCTTTCCAAGAGA+GGG | 0.540130 | 4.4:+87866617 | None:intergenic |
| TGAAATTGATCCAGTGTTGG+CGG | 0.576530 | 4.4:-87865577 | MS.gene33332:CDS |
| CAGGTGGTTTATCTGGATGG+GGG | 0.578004 | 4.4:-87865659 | MS.gene33332:CDS |
| CATTGAAATTGATCCAGTGT+TGG | 0.578009 | 4.4:-87865580 | MS.gene33332:CDS |
| CAGTGTTGGCGGAAGCACTT+GGG | 0.592959 | 4.4:-87865566 | MS.gene33332:CDS |
| AGGCACATTCTTTCCAAGAG+AGG | 0.599702 | 4.4:+87866616 | None:intergenic |
| AGAGAGGGAATATGAGTTAG+GGG | 0.621199 | 4.4:+87866632 | None:intergenic |
| TGTTTATAACCAATCTGGTG+TGG | 0.624829 | 4.4:-87865631 | MS.gene33332:CDS |
| GAGAGGGAATATGAGTTAGG+GGG | 0.675178 | 4.4:+87866633 | None:intergenic |
| ACTGATAAGACACCTAGACA+AGG | 0.730418 | 4.4:+87866596 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATCATGTTAAAAAAAAAA+AGG | + | chr4.4:87866222-87866241 | None:intergenic | 15.0% |
| !! | TTATACAATATGATGTTAAT+AGG | - | chr4.4:87866407-87866426 | MS.gene33332:intron | 15.0% |
| !!! | TCTATTTCATTAGTTTTTTT+GGG | - | chr4.4:87866340-87866359 | MS.gene33332:intron | 15.0% |
| !!! | TTCTATTTCATTAGTTTTTT+TGG | - | chr4.4:87866339-87866358 | MS.gene33332:intron | 15.0% |
| !! | AGTTTATGAGAATGAATATT+TGG | - | chr4.4:87865912-87865931 | MS.gene33332:intron | 20.0% |
| !! | CTAATTTATTTCATGTTATG+AGG | - | chr4.4:87865950-87865969 | MS.gene33332:intron | 20.0% |
| !! | GAACAAAATGATTTATTTGT+TGG | + | chr4.4:87866453-87866472 | None:intergenic | 20.0% |
| !! | TAATATTACTATCTTAGTGA+AGG | - | chr4.4:87865750-87865769 | MS.gene33332:intron | 20.0% |
| ! | AAGGTAATATTAAATGCAAC+TGG | - | chr4.4:87865862-87865881 | MS.gene33332:intron | 25.0% |
| ! | AATATTAAATGCAACTGGTT+TGG | - | chr4.4:87865867-87865886 | MS.gene33332:intron | 25.0% |
| ! | AATATTACCTTACCAAGTAT+GGG | + | chr4.4:87865853-87865872 | None:intergenic | 25.0% |
| ! | ATAGAAGAATCTTCAGTATA+AGG | + | chr4.4:87866325-87866344 | None:intergenic | 25.0% |
| ! | ATGTATCACCATAATTGATT+TGG | - | chr4.4:87865701-87865720 | MS.gene33332:intron | 25.0% |
| ! | GTATATATAGCTAGAAAGAA+TGG | - | chr4.4:87866163-87866182 | MS.gene33332:intron | 25.0% |
| ! | TAATATTACCTTACCAAGTA+TGG | + | chr4.4:87865854-87865873 | None:intergenic | 25.0% |
| ! | TTGACTAATTGCAATGTATA+AGG | + | chr4.4:87866252-87866271 | None:intergenic | 25.0% |
| AAAGGTTCTTCTGTAGTTAA+AGG | - | chr4.4:87866298-87866317 | MS.gene33332:intron | 30.0% | |
| ACAAAAAACAATTCAGCAGT+TGG | + | chr4.4:87866475-87866494 | None:intergenic | 30.0% | |
| ACTGAACAAAGCTTAGATTT+AGG | - | chr4.4:87866055-87866074 | MS.gene33332:intron | 30.0% | |
| ATGGTTGTTTATAACCAATC+TGG | - | chr4.4:87866535-87866554 | MS.gene33332:intron | 30.0% | |
| CAAACTGTCCAAATCAATTA+TGG | + | chr4.4:87865712-87865731 | None:intergenic | 30.0% | |
| CCAAGCACTTGTATTATATT+AGG | - | chr4.4:87865641-87865660 | MS.gene33332:CDS | 30.0% | |
| CCTAATATAATACAAGTGCT+TGG | + | chr4.4:87865644-87865663 | None:intergenic | 30.0% | |
| CTATAGCATACACTTAGAAT+TGG | + | chr4.4:87865671-87865690 | None:intergenic | 30.0% | |
| GATATTATGCGGAATTCTAT+GGG | + | chr4.4:87865824-87865843 | None:intergenic | 30.0% | |
| GGATGAGGTTAATGAAATTA+TGG | - | chr4.4:87865971-87865990 | MS.gene33332:intron | 30.0% | |
| GGATGCTATACTATTCAAAT+TGG | - | chr4.4:87865888-87865907 | MS.gene33332:intron | 30.0% | |
| TATTTCATGTTATGAGGATG+AGG | - | chr4.4:87865956-87865975 | MS.gene33332:intron | 30.0% | |
| TCATTGAAAAATAACTGTGC+AGG | - | chr4.4:87866381-87866400 | MS.gene33332:intron | 30.0% | |
| TGAAATCTAGATGAAGTGAT+GGG | - | chr4.4:87866004-87866023 | MS.gene33332:intron | 30.0% | |
| ! | TTTGAACGTGCTAGAATTTT+AGG | - | chr4.4:87866274-87866293 | MS.gene33332:intron | 30.0% |
| !! | AATTGATTTGGACAGTTTGT+TGG | - | chr4.4:87865713-87865732 | MS.gene33332:intron | 30.0% |
| AAGAGAGGGAATATGAGTTA+GGG | + | chr4.4:87865543-87865562 | None:intergenic | 35.0% | |
| CAACACTGGATCAATTTCAA+TGG | + | chr4.4:87866593-87866612 | None:intergenic | 35.0% | |
| CATTGAAATTGATCCAGTGT+TGG | - | chr4.4:87866591-87866610 | MS.gene33332:intron | 35.0% | |
| CTAGAAAGAATGGACAAGTT+TGG | - | chr4.4:87866173-87866192 | MS.gene33332:intron | 35.0% | |
| CTGAAATCTAGATGAAGTGA+TGG | - | chr4.4:87866003-87866022 | MS.gene33332:intron | 35.0% | |
| GGATATTATGCGGAATTCTA+TGG | + | chr4.4:87865825-87865844 | None:intergenic | 35.0% | |
| TGTTTATAACCAATCTGGTG+TGG | - | chr4.4:87866540-87866559 | MS.gene33332:intron | 35.0% | |
| TTGGAGTTCATATTCTCACA+AGG | + | chr4.4:87865625-87865644 | None:intergenic | 35.0% | |
| ! | GTTGGTAAGAGTTTCATCAT+TGG | - | chr4.4:87866114-87866133 | MS.gene33332:intron | 35.0% |
| !! | CGTGCTAGAATTTTAGGTAA+AGG | - | chr4.4:87866280-87866299 | MS.gene33332:intron | 35.0% |
| !! | GAGTTTCATCATTGGTTTCT+TGG | - | chr4.4:87866122-87866141 | MS.gene33332:intron | 35.0% |
| AAAATTGCACCACACCAGAT+TGG | + | chr4.4:87866552-87866571 | None:intergenic | 40.0% | |
| AATATCCCACTTCCCATACT+TGG | - | chr4.4:87865838-87865857 | MS.gene33332:intron | 40.0% | |
| ACTGATAAGACACCTAGACA+AGG | + | chr4.4:87865578-87865597 | None:intergenic | 40.0% | |
| AGAGAGGGAATATGAGTTAG+GGG | + | chr4.4:87865542-87865561 | None:intergenic | 40.0% | |
| ATGGGAAGTGGGATATTATG+CGG | + | chr4.4:87865835-87865854 | None:intergenic | 40.0% | |
| CAAGAGAGGGAATATGAGTT+AGG | + | chr4.4:87865544-87865563 | None:intergenic | 40.0% | |
| CTAACTCATATTCCCTCTCT+TGG | - | chr4.4:87865542-87865561 | MS.gene33332:CDS | 40.0% | |
| GTACGCAATAATCTCGTAGT+TGG | - | chr4.4:87866096-87866115 | MS.gene33332:intron | 40.0% | |
| TACAGGTGGTTTATCTGGAT+GGG | - | chr4.4:87866510-87866529 | MS.gene33332:intron | 40.0% | |
| TGCGGAATTCTATGGGTTTA+AGG | + | chr4.4:87865817-87865836 | None:intergenic | 40.0% | |
| TGCTTTACAGGTGGTTTATC+TGG | - | chr4.4:87866505-87866524 | MS.gene33332:intron | 40.0% | |
| TTACAGGTGGTTTATCTGGA+TGG | - | chr4.4:87866509-87866528 | MS.gene33332:intron | 40.0% | |
| ! | TGAAATTGATCCAGTGTTGG+CGG | - | chr4.4:87866594-87866613 | MS.gene33332:intron | 40.0% |
| ! | TGTGGTGCAATTTTCAGCTT+GGG | - | chr4.4:87866558-87866577 | MS.gene33332:intron | 40.0% |
| ACAGGTGGTTTATCTGGATG+GGG | - | chr4.4:87866511-87866530 | MS.gene33332:intron | 45.0% | |
| ACCTTACCAAGTATGGGAAG+TGG | + | chr4.4:87865847-87865866 | None:intergenic | 45.0% | |
| AGGCACATTCTTTCCAAGAG+AGG | + | chr4.4:87865558-87865577 | None:intergenic | 45.0% | |
| CCTTACCAAGTATGGGAAGT+GGG | + | chr4.4:87865846-87865865 | None:intergenic | 45.0% | |
| GAGAGGGAATATGAGTTAGG+GGG | + | chr4.4:87865541-87865560 | None:intergenic | 45.0% | |
| GGAAAGAATGTGCCTTGTCT+AGG | - | chr4.4:87865563-87865582 | MS.gene33332:CDS | 45.0% | |
| GGCACATTCTTTCCAAGAGA+GGG | + | chr4.4:87865557-87865576 | None:intergenic | 45.0% | |
| ! | GTGTGGTGCAATTTTCAGCT+TGG | - | chr4.4:87866557-87866576 | MS.gene33332:intron | 45.0% |
| ! | GTTGATGCTGCTTGCTTTAC+AGG | - | chr4.4:87866493-87866512 | MS.gene33332:intron | 45.0% |
| CAGGTGGTTTATCTGGATGG+GGG | - | chr4.4:87866512-87866531 | MS.gene33332:intron | 50.0% | |
| CCCACTTCCCATACTTGGTA+AGG | - | chr4.4:87865843-87865862 | MS.gene33332:intron | 50.0% | |
| TGGTCAGAGAGAGCTGTTCT+AGG | + | chr4.4:87866433-87866452 | None:intergenic | 50.0% | |
| TGGTTTATCTGGATGGGGGA+TGG | - | chr4.4:87866516-87866535 | MS.gene33332:intron | 50.0% | |
| ! | GATGCTGCTTGCTTTACAGG+TGG | - | chr4.4:87866496-87866515 | MS.gene33332:intron | 50.0% |
| !! | CAGTGTTGGCGGAAGCACTT+GGG | - | chr4.4:87866605-87866624 | MS.gene33332:intron | 55.0% |
| !! | GCGGAAGCACTTGGGTTAGA+TGG | - | chr4.4:87866613-87866632 | MS.gene33332:CDS | 55.0% |
| CCAAGTGCTTCCGCCAACAC+TGG | + | chr4.4:87866607-87866626 | None:intergenic | 60.0% | |
| !! | CCAGTGTTGGCGGAAGCACT+TGG | - | chr4.4:87866604-87866623 | MS.gene33332:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 87865533 | 87866660 | 87865533 | ID=MS.gene33332 |
| chr4.4 | mRNA | 87865533 | 87866660 | 87865533 | ID=MS.gene33332.t1;Parent=MS.gene33332 |
| chr4.4 | exon | 87866609 | 87866660 | 87866609 | ID=MS.gene33332.t1.exon1;Parent=MS.gene33332.t1 |
| chr4.4 | CDS | 87866609 | 87866660 | 87866609 | ID=cds.MS.gene33332.t1;Parent=MS.gene33332.t1 |
| chr4.4 | exon | 87865533 | 87865678 | 87865533 | ID=MS.gene33332.t1.exon2;Parent=MS.gene33332.t1 |
| chr4.4 | CDS | 87865533 | 87865678 | 87865533 | ID=cds.MS.gene33332.t1;Parent=MS.gene33332.t1 |
| Gene Sequence |
| Protein sequence |
