Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33978.t1 | XP_003592001.1 | 98.9 | 179 | 2 | 0 | 25 | 203 | 12 | 190 | 1.90E-82 | 315.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33978.t1 | Q09442 | 39.2 | 79 | 46 | 2 | 25 | 102 | 18 | 95 | 3.6e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33978.t1 | G7IAG6 | 98.9 | 179 | 2 | 0 | 25 | 203 | 12 | 190 | 1.3e-82 | 315.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene33978 | 0.824846 | 6.47E-54 | -1.69E-46 |
| MS.gene050689 | MS.gene33978 | 0.801163 | 1.02E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene33978 | 0.813689 | 2.25E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene33978 | 0.826705 | 2.34E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene33978 | 0.804652 | 1.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33978.t1 | MTR_1g096170 | 98.883 | 179 | 2 | 0 | 25 | 203 | 12 | 190 | 5.08e-130 | 363 |
| MS.gene33978.t1 | MTR_1g096170 | 96.196 | 184 | 2 | 1 | 25 | 203 | 12 | 195 | 7.00e-128 | 358 |
| MS.gene33978.t1 | MTR_3g065190 | 32.911 | 79 | 52 | 1 | 25 | 103 | 30 | 107 | 4.36e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33978.t1 | AT4G10110 | 52.406 | 187 | 53 | 5 | 25 | 201 | 12 | 172 | 1.86e-52 | 166 |
| MS.gene33978.t1 | AT2G18510 | 30.380 | 79 | 54 | 1 | 25 | 103 | 30 | 107 | 2.00e-11 | 62.4 |
Find 52 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTATAGTCGGAGAGCTTT+TGG | 0.185399 | 1.4:-74724745 | MS.gene33978:CDS |
| GACTATAGTTGGAGAGCTTT+TGG | 0.218783 | 1.4:-74724830 | MS.gene33978:intron |
| TAGTATGGTGCATCTACTTT+TGG | 0.251360 | 1.4:-74725864 | MS.gene33978:CDS |
| TGGTTACTTGTATTTAATTC+AGG | 0.266204 | 1.4:-74725832 | MS.gene33978:CDS |
| GTAGTAGCTGTAGAACTATT+TGG | 0.272346 | 1.4:+74725133 | None:intergenic |
| TAGTAGCTGTAGAACTATTT+GGG | 0.310776 | 1.4:+74725134 | None:intergenic |
| TGTATTTAATTCAGGCAATC+TGG | 0.329922 | 1.4:-74725824 | MS.gene33978:CDS |
| TCTGCAAAGGCAAAACCTTT+TGG | 0.329956 | 1.4:+74725706 | None:intergenic |
| TCTGCTAATAAACAGATATC+TGG | 0.354013 | 1.4:-74725172 | MS.gene33978:intron |
| TGCATCAGGGCGACGTGGCC+TGG | 0.360741 | 1.4:+74724696 | None:intergenic |
| TGGATAACGCTAGCCGTTCC+AGG | 0.375993 | 1.4:-74724714 | MS.gene33978:CDS |
| GAGATTGGGCTAACTGCATC+AGG | 0.387744 | 1.4:+74724682 | None:intergenic |
| TCACTTTGGTGGCAACCATT+TGG | 0.393886 | 1.4:-74724767 | MS.gene33978:CDS |
| AAGAACGCACCGCAAACTTC+AGG | 0.422993 | 1.4:+74725611 | None:intergenic |
| CTATTTATTTGCACAGGGTA+AGG | 0.429684 | 1.4:+74725079 | None:intergenic |
| TCTGAACTATTTATTTGCAC+AGG | 0.438610 | 1.4:+74725073 | None:intergenic |
| ATTTGCACAGGGTAAGGCCT+TGG | 0.443958 | 1.4:+74725085 | None:intergenic |
| ACACCTCTGTGTACGTAGTA+TGG | 0.447511 | 1.4:-74725879 | MS.gene33978:CDS |
| CATTAGATTGGTCGGTTGCA+CGG | 0.455879 | 1.4:+74724800 | None:intergenic |
| CTCTCCGACTATAGTCCAAA+TGG | 0.467158 | 1.4:+74724752 | None:intergenic |
| CTTGCACATTCCTAAAGATA+AGG | 0.486567 | 1.4:-74725740 | MS.gene33978:CDS |
| TGGATGAGAGGGTGACAGAC+AGG | 0.491893 | 1.4:-74725804 | MS.gene33978:CDS |
| GAGATCCATACGCATTAGAT+TGG | 0.492253 | 1.4:+74724788 | None:intergenic |
| TATGATATATTAATTCAAGC+TGG | 0.514163 | 1.4:-74725775 | MS.gene33978:CDS |
| TTCGTTTGTACAGTGTTACA+AGG | 0.518910 | 1.4:+74725635 | None:intergenic |
| GCACGATCTGAGAATCGGTC+AGG | 0.520914 | 1.4:+74725019 | None:intergenic |
| TCCATACGCATTAGATTGGT+CGG | 0.528133 | 1.4:+74724792 | None:intergenic |
| CGTGCGTTAAATTATTCTCA+AGG | 0.530851 | 1.4:-74725001 | MS.gene33978:intron |
| CAAACGAACCCTGAAGTTTG+CGG | 0.533929 | 1.4:-74725620 | MS.gene33978:intron |
| ACCGACCAATCTAATGCGTA+TGG | 0.537772 | 1.4:-74724793 | MS.gene33978:CDS |
| CTCAGTTTCATATTCTGCAA+AGG | 0.546183 | 1.4:+74725693 | None:intergenic |
| AATGCGTATGGATCTCACTT+TGG | 0.547698 | 1.4:-74724781 | MS.gene33978:CDS |
| CAGGGCGACGTGGCCTGGAA+CGG | 0.550905 | 1.4:+74724701 | None:intergenic |
| GCGTATGGATCTCACTTTGG+TGG | 0.555553 | 1.4:-74724778 | MS.gene33978:CDS |
| CTGAACTATTTATTTGCACA+GGG | 0.556003 | 1.4:+74725074 | None:intergenic |
| AGAACGCACCGCAAACTTCA+GGG | 0.575012 | 1.4:+74725612 | None:intergenic |
| TTAACGCACGATCTGAGAAT+CGG | 0.575936 | 1.4:+74725014 | None:intergenic |
| CTAATAAACAGATATCTGGA+AGG | 0.576973 | 1.4:-74725168 | MS.gene33978:intron |
| TTCAGGCAATCTGGATGAGA+GGG | 0.588517 | 1.4:-74725815 | MS.gene33978:CDS |
| ATTCAGGCAATCTGGATGAG+AGG | 0.594652 | 1.4:-74725816 | MS.gene33978:CDS |
| GCAACCATTTGGACTATAGT+CGG | 0.610307 | 1.4:-74724756 | MS.gene33978:CDS |
| CAAATTCTTCTCAACGACCA+AGG | 0.631237 | 1.4:-74725102 | MS.gene33978:CDS |
| CTAACTGCATCAGGGCGACG+TGG | 0.635502 | 1.4:+74724691 | None:intergenic |
| TAATAAACAGATATCTGGAA+GGG | 0.642791 | 1.4:-74725167 | MS.gene33978:intron |
| AGTAGCTGTAGAACTATTTG+GGG | 0.648842 | 1.4:+74725135 | None:intergenic |
| ATTAATTCAAGCTGGACGAG+TGG | 0.649212 | 1.4:-74725767 | MS.gene33978:CDS |
| CTTTGCAGAATATGAAACTG+AGG | 0.657421 | 1.4:-74725692 | MS.gene33978:CDS |
| AGATTGGGCTAACTGCATCA+GGG | 0.658192 | 1.4:+74724683 | None:intergenic |
| CGTTGAGAAGAATTTGACGA+GGG | 0.669321 | 1.4:+74725109 | None:intergenic |
| TCGTTGAGAAGAATTTGACG+AGG | 0.688771 | 1.4:+74725108 | None:intergenic |
| GTCAGGTATTGATTGCCTAG+CGG | 0.723907 | 1.4:+74725036 | None:intergenic |
| GCACCATACTACGTACACAG+AGG | 0.797741 | 1.4:+74725876 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATATATTAATTCAAGC+TGG | - | chr1.4:74724804-74724823 | MS.gene33978:CDS | 20.0% |
| !! | TATTAAACGAAGTTAATAGT+TGG | - | chr1.4:74725154-74725173 | MS.gene33978:CDS | 20.0% |
| !! | TTTAAATTTCTTTCATGTCA+TGG | - | chr1.4:74725361-74725380 | MS.gene33978:intron | 20.0% |
| !!! | TTGCATAAAATATTTTCAAC+AGG | + | chr1.4:74725131-74725150 | None:intergenic | 20.0% |
| ! | AGCTGTATTTATTACATGAT+TGG | + | chr1.4:74725274-74725293 | None:intergenic | 25.0% |
| ! | TAATAAACAGATATCTGGAA+GGG | - | chr1.4:74725412-74725431 | MS.gene33978:intron | 25.0% |
| ! | TGGTTACTTGTATTTAATTC+AGG | - | chr1.4:74724747-74724766 | MS.gene33978:CDS | 25.0% |
| ! | TTAATTCAATCTAATGCGTA+TGG | - | chr1.4:74725701-74725720 | MS.gene33978:CDS | 25.0% |
| !! | ATGTCATGGTTATTTGTTAA+TGG | - | chr1.4:74725375-74725394 | MS.gene33978:intron | 25.0% |
| !! | TATTGTGTGAATTACTGATT+TGG | - | chr1.4:74725010-74725029 | MS.gene33978:CDS | 25.0% |
| !! | TTTTCAGATTCCTTATCTTT+AGG | + | chr1.4:74724852-74724871 | None:intergenic | 25.0% |
| AAGGAATCTGAAAAACCAAA+AGG | - | chr1.4:74724858-74724877 | MS.gene33978:intron | 30.0% | |
| CTAATAAACAGATATCTGGA+AGG | - | chr1.4:74725411-74725430 | MS.gene33978:intron | 30.0% | |
| CTGAACTATTTATTTGCACA+GGG | + | chr1.4:74725508-74725527 | None:intergenic | 30.0% | |
| GAAGTTAATAGTTGGATACT+TGG | - | chr1.4:74725162-74725181 | MS.gene33978:intron | 30.0% | |
| TAGTAGCTGTAGAACTATTT+GGG | + | chr1.4:74725448-74725467 | None:intergenic | 30.0% | |
| TATAAAGCTTTGCTGAATAG+AGG | - | chr1.4:74725064-74725083 | MS.gene33978:CDS | 30.0% | |
| TCATGTAATAAATACAGCTC+AGG | - | chr1.4:74725275-74725294 | MS.gene33978:intron | 30.0% | |
| TCTGAACTATTTATTTGCAC+AGG | + | chr1.4:74725509-74725528 | None:intergenic | 30.0% | |
| TCTGCTAATAAACAGATATC+TGG | - | chr1.4:74725407-74725426 | MS.gene33978:intron | 30.0% | |
| TGCATCTATAAAGTTGAAAG+CGG | - | chr1.4:74725186-74725205 | MS.gene33978:intron | 30.0% | |
| TGTATTTAATTCAGGCAATC+TGG | - | chr1.4:74724755-74724774 | MS.gene33978:CDS | 30.0% | |
| TGTTAACTTTCGCTATAGTT+GGG | + | chr1.4:74725224-74725243 | None:intergenic | 30.0% | |
| AGTAGCTGTAGAACTATTTG+GGG | + | chr1.4:74725447-74725466 | None:intergenic | 35.0% | |
| CGTGCGTTAAATTATTCTCA+AGG | - | chr1.4:74725578-74725597 | MS.gene33978:intron | 35.0% | |
| CTATTTATTTGCACAGGGTA+AGG | + | chr1.4:74725503-74725522 | None:intergenic | 35.0% | |
| CTCAGTTTCATATTCTGCAA+AGG | + | chr1.4:74724889-74724908 | None:intergenic | 35.0% | |
| CTGTTAACTTTCGCTATAGT+TGG | + | chr1.4:74725225-74725244 | None:intergenic | 35.0% | |
| CTTGCACATTCCTAAAGATA+AGG | - | chr1.4:74724839-74724858 | MS.gene33978:intron | 35.0% | |
| CTTTGCAGAATATGAAACTG+AGG | - | chr1.4:74724887-74724906 | MS.gene33978:intron | 35.0% | |
| GTAGTAGCTGTAGAACTATT+TGG | + | chr1.4:74725449-74725468 | None:intergenic | 35.0% | |
| TCAGTAATTCACACAATACG+TGG | + | chr1.4:74725008-74725027 | None:intergenic | 35.0% | |
| TTACATGATTGGAAGTTCAC+AGG | + | chr1.4:74725263-74725282 | None:intergenic | 35.0% | |
| TTCGTTTGTACAGTGTTACA+AGG | + | chr1.4:74724947-74724966 | None:intergenic | 35.0% | |
| ! | AAAATTTTCAGCATTCCGCT+AGG | - | chr1.4:74725528-74725547 | MS.gene33978:intron | 35.0% |
| ! | ACTATAGTTGGAGAGCTTTT+GGG | - | chr1.4:74725750-74725769 | MS.gene33978:CDS | 35.0% |
| ! | TAGTATGGTGCATCTACTTT+TGG | - | chr1.4:74724715-74724734 | MS.gene33978:CDS | 35.0% |
| !! | AGTATGGTGCATCTACTTTT+GGG | - | chr1.4:74724716-74724735 | MS.gene33978:CDS | 35.0% |
| !!! | TCTACTTTTGGGTTCTGATT+TGG | - | chr1.4:74724727-74724746 | MS.gene33978:CDS | 35.0% |
| AATGCGTATGGATCTCACTT+TGG | - | chr1.4:74725713-74725732 | MS.gene33978:CDS | 40.0% | |
| ATTAATTCAAGCTGGACGAG+TGG | - | chr1.4:74724812-74724831 | MS.gene33978:CDS | 40.0% | |
| CAAATTCTTCTCAACGACCA+AGG | - | chr1.4:74725477-74725496 | MS.gene33978:intron | 40.0% | |
| CGTTGAGAAGAATTTGACGA+GGG | + | chr1.4:74725473-74725492 | None:intergenic | 40.0% | |
| CTCTCCAACTATAGTCCAAA+TGG | + | chr1.4:74725745-74725764 | None:intergenic | 40.0% | |
| GAGATCCATACGCATTAGAT+TGG | + | chr1.4:74725709-74725728 | None:intergenic | 40.0% | |
| GCAACCATTTGGACTATAGT+CGG | - | chr1.4:74725738-74725757 | MS.gene33978:CDS | 40.0% | |
| GCAACCATTTGGACTATAGT+TGG | - | chr1.4:74725738-74725757 | MS.gene33978:CDS | 40.0% | |
| TCCATACGCATTAGATTGGT+CGG | + | chr1.4:74725790-74725809 | None:intergenic | 40.0% | |
| TCGTTGAGAAGAATTTGACG+AGG | + | chr1.4:74725474-74725493 | None:intergenic | 40.0% | |
| TTAACGCACGATCTGAGAAT+CGG | + | chr1.4:74725568-74725587 | None:intergenic | 40.0% | |
| TTTGCTGAATAGAGGTTCAC+AGG | - | chr1.4:74725072-74725091 | MS.gene33978:CDS | 40.0% | |
| ! | ACTATAGTCGGAGAGCTTTT+GGG | - | chr1.4:74725835-74725854 | MS.gene33978:CDS | 40.0% |
| ! | CTATAGTTGGAGAGCTTTTG+GGG | - | chr1.4:74725751-74725770 | MS.gene33978:CDS | 40.0% |
| ! | GACTATAGTTGGAGAGCTTT+TGG | - | chr1.4:74725749-74725768 | MS.gene33978:CDS | 40.0% |
| ! | TATGCTGTCAGACTTTTCTC+AGG | - | chr1.4:74724921-74724940 | MS.gene33978:intron | 40.0% |
| ! | TCTGCAAAGGCAAAACCTTT+TGG | + | chr1.4:74724876-74724895 | None:intergenic | 40.0% |
| ACACCTCTGTGTACGTAGTA+TGG | - | chr1.4:74724700-74724719 | MS.gene33978:CDS | 45.0% | |
| ACCGACCAATCTAATGCGTA+TGG | - | chr1.4:74725786-74725805 | MS.gene33978:CDS | 45.0% | |
| ATTCAGGCAATCTGGATGAG+AGG | - | chr1.4:74724763-74724782 | MS.gene33978:CDS | 45.0% | |
| CAAACGAACCCTGAAGTTTG+CGG | - | chr1.4:74724959-74724978 | MS.gene33978:intron | 45.0% | |
| CTCTCCGACTATAGTCCAAA+TGG | + | chr1.4:74725830-74725849 | None:intergenic | 45.0% | |
| TTCAGGCAATCTGGATGAGA+GGG | - | chr1.4:74724764-74724783 | MS.gene33978:CDS | 45.0% | |
| ! | CATTAGATTGGTCGGTTGCA+CGG | + | chr1.4:74725782-74725801 | None:intergenic | 45.0% |
| ! | CTATAGTCGGAGAGCTTTTG+GGG | - | chr1.4:74725836-74725855 | MS.gene33978:CDS | 45.0% |
| ! | GACTATAGTCGGAGAGCTTT+TGG | - | chr1.4:74725834-74725853 | MS.gene33978:CDS | 45.0% |
| ! | TCACTTTGGTGGCAACCATT+TGG | - | chr1.4:74725727-74725746 | MS.gene33978:CDS | 45.0% |
| !! | GTCAGGTATTGATTGCCTAG+CGG | + | chr1.4:74725546-74725565 | None:intergenic | 45.0% |
| AAGAACGCACCGCAAACTTC+AGG | + | chr1.4:74724971-74724990 | None:intergenic | 50.0% | |
| ACCTATGCAGCCCAGGTATA+CGG | + | chr1.4:74725304-74725323 | None:intergenic | 50.0% | |
| AGAACGCACCGCAAACTTCA+GGG | + | chr1.4:74724970-74724989 | None:intergenic | 50.0% | |
| AGGTTCACAGGCTGAGACAT+TGG | - | chr1.4:74725084-74725103 | MS.gene33978:CDS | 50.0% | |
| ATTTGCACAGGGTAAGGCCT+TGG | + | chr1.4:74725497-74725516 | None:intergenic | 50.0% | |
| CAATCAAACCTATGCAGCCC+AGG | + | chr1.4:74725311-74725330 | None:intergenic | 50.0% | |
| GCACCATACTACGTACACAG+AGG | + | chr1.4:74724706-74724725 | None:intergenic | 50.0% | |
| GCGTATGGATCTCACTTTGG+TGG | - | chr1.4:74725716-74725735 | MS.gene33978:CDS | 50.0% | |
| !! | TAGTTGGAGAGCTTTTGGGG+AGG | - | chr1.4:74725754-74725773 | MS.gene33978:CDS | 50.0% |
| ACCGTATACCTGGGCTGCAT+AGG | - | chr1.4:74725300-74725319 | MS.gene33978:intron | 55.0% | |
| AGCTCAGGACACCGTATACC+TGG | - | chr1.4:74725290-74725309 | MS.gene33978:intron | 55.0% | |
| GCACGATCTGAGAATCGGTC+AGG | + | chr1.4:74725563-74725582 | None:intergenic | 55.0% | |
| GCTCAGGACACCGTATACCT+GGG | - | chr1.4:74725291-74725310 | MS.gene33978:intron | 55.0% | |
| TGGATAACGCTAGCCGTTCC+AGG | - | chr1.4:74725865-74725884 | MS.gene33978:CDS | 55.0% | |
| TGGATGAGAGGGTGACAGAC+AGG | - | chr1.4:74724775-74724794 | MS.gene33978:CDS | 55.0% | |
| !! | GAGAGCTTTTGGGGAGGAAC+TGG | - | chr1.4:74725760-74725779 | MS.gene33978:CDS | 55.0% |
| !! | TAGTCGGAGAGCTTTTGGGG+AGG | - | chr1.4:74725839-74725858 | MS.gene33978:CDS | 55.0% |
| CAGGGCGACGTGGCCTGGAA+CGG | + | chr1.4:74725881-74725900 | None:intergenic | 70.0% | |
| TGCATCAGGGCGACGTGGCC+TGG | + | chr1.4:74725886-74725905 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 74724696 | 74725905 | 74724696 | ID=MS.gene33978 |
| chr1.4 | mRNA | 74724696 | 74725905 | 74724696 | ID=MS.gene33978.t1;Parent=MS.gene33978 |
| chr1.4 | exon | 74725621 | 74725905 | 74725621 | ID=MS.gene33978.t1.exon1;Parent=MS.gene33978.t1 |
| chr1.4 | CDS | 74725621 | 74725905 | 74725621 | ID=cds.MS.gene33978.t1;Parent=MS.gene33978.t1 |
| chr1.4 | exon | 74725002 | 74725179 | 74725002 | ID=MS.gene33978.t1.exon2;Parent=MS.gene33978.t1 |
| chr1.4 | CDS | 74725002 | 74725179 | 74725002 | ID=cds.MS.gene33978.t1;Parent=MS.gene33978.t1 |
| chr1.4 | exon | 74724696 | 74724844 | 74724696 | ID=MS.gene33978.t1.exon3;Parent=MS.gene33978.t1 |
| chr1.4 | CDS | 74724696 | 74724844 | 74724696 | ID=cds.MS.gene33978.t1;Parent=MS.gene33978.t1 |
| Gene Sequence |
| Protein sequence |
