AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34001

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34001.t1 MTR_1g095830 97.740 177 4 0 1 177 1 177 4.36e-128 357
MS.gene34001.t1 MTR_1g095830 95.105 143 5 1 1 143 1 141 4.49e-96 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34001.t1 AT3G52300 80.952 168 32 0 1 168 1 168 3.16e-101 288
MS.gene34001.t1 AT3G52300 78.704 108 23 0 1 108 1 108 8.38e-62 187

Find 39 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATAATATTCCCAGTCTA+TGG 0.167189 1.4:+74435521 None:intergenic
CTTACCAGTGCATCAAATTT+AGG 0.208576 1.4:+74433507 None:intergenic
AGGAGATTAGGAACGATAAC+TGG 0.292837 1.4:-74433164 MS.gene34001:CDS
GCTGAAGTTGGTTGAGCTTA+AGG 0.315735 1.4:-74433407 MS.gene34001:intron
GTCCCAATCGATCTTCTTCC+CGG 0.344356 1.4:+74436271 None:intergenic
TCTGAAAGAATCTGAACGTT+TGG 0.374930 1.4:-74433368 MS.gene34001:CDS
AAAAGGAATTGGCACTCGTT+TGG 0.433018 1.4:-74435499 MS.gene34001:CDS
AGCAATTTGATGAGGAGATT+AGG 0.438102 1.4:-74433176 MS.gene34001:CDS
TCAAATTGCTTCTTCAGCTC+AGG 0.438466 1.4:+74433189 None:intergenic
AGTTACTGTGTCAACAAACT+TGG 0.447924 1.4:+74433542 None:intergenic
GACTGGGAATATTATAGAAA+AGG 0.455154 1.4:-74435516 MS.gene34001:CDS
GAATATTATAGAAAAGGAAT+TGG 0.458956 1.4:-74435510 MS.gene34001:CDS
TAAGCCTAAATTTGATGCAC+TGG 0.467869 1.4:-74433511 MS.gene34001:intron
AGGAGCCTGAGCCCATAGAC+TGG 0.471648 1.4:-74435533 MS.gene34001:CDS
AGAATCTGAACGTTTGGAAA+AGG 0.472141 1.4:-74433362 MS.gene34001:CDS
CTATAATATTCCCAGTCTAT+GGG 0.473165 1.4:+74435522 None:intergenic
AAATTCACGACGAGCTTCAT+CGG 0.482178 1.4:+74436226 None:intergenic
ACAGATGTTGCATTCAAAGC+CGG 0.489390 1.4:-74436290 MS.gene34001:CDS
TCAAATTTAGGCTTATATTG+AGG 0.499518 1.4:+74433519 None:intergenic
AGGAATTGGCACTCGTTTGG+TGG 0.500877 1.4:-74435496 MS.gene34001:CDS
CTTCTTCAGCTCAGGATGCT+CGG 0.501457 1.4:+74433197 None:intergenic
CAGATGTTGCATTCAAAGCC+GGG 0.515624 1.4:-74436289 MS.gene34001:CDS
TATTCCCAGTCTATGGGCTC+AGG 0.528775 1.4:+74435528 None:intergenic
AAGCCGGGAAGAAGATCGAT+TGG 0.530458 1.4:-74436274 MS.gene34001:CDS
GGAAGAAGATCGATTGGGAC+GGG 0.534761 1.4:-74436268 MS.gene34001:CDS
AAAGTACTCATCTGCTGTCA+TGG 0.536378 1.4:+74433221 None:intergenic
GGAGATTAGGAACGATAACT+GGG 0.553647 1.4:-74433163 MS.gene34001:CDS
GTTACTGTGTCAACAAACTT+GGG 0.562398 1.4:+74433543 None:intergenic
AGCCGGGAAGAAGATCGATT+GGG 0.567684 1.4:-74436273 MS.gene34001:CDS
GGAGCCTGAGCCCATAGACT+GGG 0.568751 1.4:-74435532 MS.gene34001:CDS
GAAGATCGATTGGGACGGGA+TGG 0.577380 1.4:-74436264 MS.gene34001:CDS
TGCTAATGTACAGTCGCTGA+AGG 0.578326 1.4:-74433335 MS.gene34001:intron
GGTTGAGCTTAAGGAAGCAG+AGG 0.581516 1.4:-74433398 MS.gene34001:CDS
ACTCGAAACAAAGTTCAGCC+AGG 0.598938 1.4:-74436165 MS.gene34001:intron
AAAGCGAAAGCGAAAATGAG+CGG 0.621372 1.4:-74436329 None:intergenic
GGGAAGAAGATCGATTGGGA+CGG 0.627371 1.4:-74436269 MS.gene34001:CDS
GCTGAAGAAGCAATTTGATG+AGG 0.630857 1.4:-74433184 MS.gene34001:CDS
TTACTGTGTCAACAAACTTG+GGG 0.648687 1.4:+74433544 None:intergenic
GAGATTAGGAACGATAACTG+GGG 0.757484 1.4:-74433162 MS.gene34001:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAAATTATTTTTTTTAT+TGG - chr1.4:74434703-74434722 MS.gene34001:intron 0.0%
!! TAAAAAAATACTAATATAAG+GGG + chr1.4:74434023-74434042 None:intergenic 10.0%
!! TAAAAATGAGAAATTAATAT+AGG - chr1.4:74434593-74434612 MS.gene34001:intron 10.0%
!!! ATATATTCTAATGTAAATTT+TGG + chr1.4:74435398-74435417 None:intergenic 10.0%
!!! TTTTTTTAATCTGTATTTAA+TGG - chr1.4:74434829-74434848 MS.gene34001:intron 10.0%
!! AAATAACAATATATTGAATG+AGG + chr1.4:74435535-74435554 None:intergenic 15.0%
!! AATACATAATCTATTTGTAT+AGG + chr1.4:74433627-74433646 None:intergenic 15.0%
!!! AATTATTTCTCAAATTGAAA+AGG - chr1.4:74435454-74435473 MS.gene34001:intron 15.0%
!!! AGTATTTTTTTATTTGTCAA+TGG - chr1.4:74434031-74434050 MS.gene34001:intron 15.0%
!!! TACTGTAAATATTTGTATTT+TGG + chr1.4:74434753-74434772 None:intergenic 15.0%
!! AACATCAAAAGTTGAATTAA+TGG + chr1.4:74435853-74435872 None:intergenic 20.0%
!! AATAACTTGACAACAAAAAT+TGG + chr1.4:74434682-74434701 None:intergenic 20.0%
!! AATGGTATGTAAAATTTGAA+TGG + chr1.4:74435835-74435854 None:intergenic 20.0%
!! AGTTATTGCCTAAATATTTA+TGG - chr1.4:74435750-74435769 MS.gene34001:intron 20.0%
!! GAATATTATAGAAAAGGAAT+TGG - chr1.4:74433932-74433951 MS.gene34001:intron 20.0%
!! TCATAATTATATAACTGAAC+TGG + chr1.4:74435144-74435163 None:intergenic 20.0%
!!! GTTAAATTTTAACATGCATT+AGG + chr1.4:74435374-74435393 None:intergenic 20.0%
!!! GTTTTATAGTTGTTTTTTTC+AGG - chr1.4:74435231-74435250 MS.gene34001:intron 20.0%
!!! TAATTTGATGACTACATAAT+TGG + chr1.4:74433807-74433826 None:intergenic 20.0%
!!! TTCTCAGTTTTATGTAATAT+TGG - chr1.4:74436151-74436170 MS.gene34001:intron 20.0%
! AATGTCTATCAATTTCAGAT+AGG + chr1.4:74435305-74435324 None:intergenic 25.0%
! AATTATGAACCATAGTTTAC+TGG - chr1.4:74435156-74435175 MS.gene34001:intron 25.0%
! AGATTATGTATTTAGTTACG+AGG - chr1.4:74433635-74433654 MS.gene34001:intron 25.0%
! AGGATATTGATAAATTCAAC+TGG - chr1.4:74434156-74434175 MS.gene34001:intron 25.0%
! ATGTCTATCAATTTCAGATA+GGG + chr1.4:74435304-74435323 None:intergenic 25.0%
! CATTAGAATATATGAAACTG+TGG - chr1.4:74435405-74435424 MS.gene34001:intron 25.0%
! GACTACATCCATAAATATTT+AGG + chr1.4:74435761-74435780 None:intergenic 25.0%
! GTATTCAAAACATGATGAAA+AGG + chr1.4:74433597-74433616 None:intergenic 25.0%
! TAAAACCTGGAAATTGTATA+TGG - chr1.4:74435573-74435592 MS.gene34001:intron 25.0%
! TATCATTTCTTATTAATCGC+AGG - chr1.4:74433889-74433908 MS.gene34001:intron 25.0%
! TCAAATTTAGGCTTATATTG+AGG + chr1.4:74435926-74435945 None:intergenic 25.0%
! TGTAGTCATCAAATTATTAC+CGG - chr1.4:74433811-74433830 MS.gene34001:intron 25.0%
!! AAAAAGATAATCTTGATAGC+TGG + chr1.4:74434998-74435017 None:intergenic 25.0%
!! AGCTATCAAGATTATCTTTT+TGG - chr1.4:74434997-74435016 MS.gene34001:intron 25.0%
!! CTTCATCATCAATTTTGAAT+TGG - chr1.4:74433463-74433482 MS.gene34001:intron 25.0%
!! TTTTCTGTGAAAACAAACAA+TGG + chr1.4:74436192-74436211 None:intergenic 25.0%
!!! AGTTCAGTTTTTTTAAGGAT+CGG + chr1.4:74434317-74434336 None:intergenic 25.0%
!!! ATCATGTTTTAGTGCATTTT+CGG - chr1.4:74433495-74433514 MS.gene34001:intron 25.0%
!!! ATTGTTTGTTTTCACAGAAA+AGG - chr1.4:74436191-74436210 MS.gene34001:CDS 25.0%
!!! CAAACAGTTCAGTTTTTTTA+AGG + chr1.4:74434322-74434341 None:intergenic 25.0%
AATTATATAACTGAACTGGC+TGG + chr1.4:74435140-74435159 None:intergenic 30.0%
ACCAAAAAAACTGAACTGTA+CGG - chr1.4:74434277-74434296 MS.gene34001:intron 30.0%
AGCATCTAAGAACAAAATGA+AGG + chr1.4:74433368-74433387 None:intergenic 30.0%
CTATAATATTCCCAGTCTAT+GGG + chr1.4:74433923-74433942 None:intergenic 30.0%
CTGATACTTAATAGCTTGAA+CGG + chr1.4:74435202-74435221 None:intergenic 30.0%
CTTGATTGATAGAGAAAAGT+TGG + chr1.4:74435982-74436001 None:intergenic 30.0%
GACTGGGAATATTATAGAAA+AGG - chr1.4:74433926-74433945 MS.gene34001:intron 30.0%
GGTATGTAAAATTTGAATGG+CGG + chr1.4:74435832-74435851 None:intergenic 30.0%
GTACCATTGAATACAAGAAT+CGG + chr1.4:74434184-74434203 None:intergenic 30.0%
GTTATTTGACCTATTCACAT+GGG - chr1.4:74435548-74435567 MS.gene34001:intron 30.0%
TAAGCAAAACTGCAACTAAT+GGG + chr1.4:74435116-74435135 None:intergenic 30.0%
TCTATAATATTCCCAGTCTA+TGG + chr1.4:74433924-74433943 None:intergenic 30.0%
TCTTCCCTATGAAAAGAATA+TGG + chr1.4:74434206-74434225 None:intergenic 30.0%
TGATACCATATACAATTTCC+AGG + chr1.4:74435581-74435600 None:intergenic 30.0%
TGCGAATGACAAAAAGATAT+TGG + chr1.4:74435037-74435056 None:intergenic 30.0%
TGTCTATCAATTTCAGATAG+GGG + chr1.4:74435303-74435322 None:intergenic 30.0%
! AAAGCAGTTTTACAAACAGT+TGG - chr1.4:74434634-74434653 MS.gene34001:intron 30.0%
! ATATATGAAACTGTGGATTG+TGG - chr1.4:74435412-74435431 MS.gene34001:intron 30.0%
! GGTACCATATTCTTTTCATA+GGG - chr1.4:74434199-74434218 MS.gene34001:intron 30.0%
! TCGTGCATTGAATTGATTTA+AGG - chr1.4:74435672-74435691 MS.gene34001:intron 30.0%
! TGGTACCATATTCTTTTCAT+AGG - chr1.4:74434198-74434217 MS.gene34001:intron 30.0%
! TGTTATTTGACCTATTCACA+TGG - chr1.4:74435547-74435566 MS.gene34001:intron 30.0%
!! ACTGAACTACTTTTGAAGAA+TGG + chr1.4:74434395-74434414 None:intergenic 30.0%
!!! GTTCAGTTTTTTTAAGGATC+GGG + chr1.4:74434316-74434335 None:intergenic 30.0%
!!! TTCAGTTTTTTTAAGGATCG+GGG + chr1.4:74434315-74434334 None:intergenic 30.0%
!!! TTGTTTTTTTCAGGTCAGTT+AGG - chr1.4:74435240-74435259 MS.gene34001:intron 30.0%
AGAATCTGAACGTTTGGAAA+AGG - chr1.4:74436080-74436099 MS.gene34001:intron 35.0%
AGCAATTTGATGAGGAGATT+AGG - chr1.4:74436266-74436285 MS.gene34001:CDS 35.0%
AGGTAATAGAAATCACCACA+TGG - chr1.4:74436127-74436146 MS.gene34001:intron 35.0%
AGTTACTGTGTCAACAAACT+TGG + chr1.4:74435903-74435922 None:intergenic 35.0%
ATACCAACACTTCTGTTTGA+AGG - chr1.4:74433722-74433741 MS.gene34001:intron 35.0%
ATGAGATGTCAATTGTGCTA+AGG + chr1.4:74434569-74434588 None:intergenic 35.0%
ATGCACGAGTAATAAATGCA+AGG + chr1.4:74435660-74435679 None:intergenic 35.0%
ATGCACTTTCTCTTCTTTAG+GGG - chr1.4:74434121-74434140 MS.gene34001:intron 35.0%
ATTCACATGGGAATAAAACC+TGG - chr1.4:74435560-74435579 MS.gene34001:intron 35.0%
ATTGAATGAGGCAAAGTTCT+AGG + chr1.4:74435523-74435542 None:intergenic 35.0%
CATAAAACTGAGAAACCATG+TGG + chr1.4:74436145-74436164 None:intergenic 35.0%
CATGCACTTTCTCTTCTTTA+GGG - chr1.4:74434120-74434139 MS.gene34001:intron 35.0%
CTAAGCAAAACTGCAACTAA+TGG + chr1.4:74435117-74435136 None:intergenic 35.0%
CTTACCAGTGCATCAAATTT+AGG + chr1.4:74435938-74435957 None:intergenic 35.0%
GAAATTAGAGAGATCTGTGA+AGG + chr1.4:74433313-74433332 None:intergenic 35.0%
GATCCGATTCTTGTATTCAA+TGG - chr1.4:74434178-74434197 MS.gene34001:intron 35.0%
GTTACTGTGTCAACAAACTT+GGG + chr1.4:74435902-74435921 None:intergenic 35.0%
TAAGCCTAAATTTGATGCAC+TGG - chr1.4:74435931-74435950 MS.gene34001:intron 35.0%
TAATTCACAAAAGCCATCCT+CGG - chr1.4:74434234-74434253 MS.gene34001:intron 35.0%
TACTTTGGTCCAGTAAACTA+TGG + chr1.4:74435168-74435187 None:intergenic 35.0%
TATTTAGTTACGAGGCTTAG+TGG - chr1.4:74433643-74433662 MS.gene34001:intron 35.0%
TCATGCACTTTCTCTTCTTT+AGG - chr1.4:74434119-74434138 MS.gene34001:intron 35.0%
TCTGAAAGAATCTGAACGTT+TGG - chr1.4:74436074-74436093 MS.gene34001:intron 35.0%
TGAGATGTCAATTGTGCTAA+GGG + chr1.4:74434568-74434587 None:intergenic 35.0%
TGATGTTATGCTTCTTCTGT+AGG - chr1.4:74435866-74435885 MS.gene34001:intron 35.0%
TTACTGTGTCAACAAACTTG+GGG + chr1.4:74435901-74435920 None:intergenic 35.0%
! ATGTGTTTTCTTGCTGAAGT+TGG - chr1.4:74436023-74436042 MS.gene34001:intron 35.0%
! GTAAAATTTGAATGGCGGAA+TGG + chr1.4:74435827-74435846 None:intergenic 35.0%
!! TGCTAGATCGATTGACTTTT+AGG - chr1.4:74433384-74433403 MS.gene34001:CDS 35.0%
!! TTAGAGCATGACTTGTTTGA+TGG - chr1.4:74434070-74434089 MS.gene34001:intron 35.0%
!!! GGTTTTATTCCCATGTGAAT+AGG + chr1.4:74435560-74435579 None:intergenic 35.0%
!!! TCCGTACAGTTCAGTTTTTT+TGG + chr1.4:74434281-74434300 None:intergenic 35.0%
AAAAGCCATCCTCGGAAATT+AGG - chr1.4:74434242-74434261 MS.gene34001:intron 40.0%
AAAAGGAATTGGCACTCGTT+TGG - chr1.4:74433943-74433962 MS.gene34001:intron 40.0%
AAAGCCATCCTCGGAAATTA+GGG - chr1.4:74434243-74434262 MS.gene34001:intron 40.0%
AAATTCACGACGAGCTTCAT+CGG + chr1.4:74433219-74433238 None:intergenic 40.0%
ACAGATGTTGCATTCAAAGC+CGG - chr1.4:74433152-74433171 MS.gene34001:CDS 40.0%
ACTGGACCAAAGTACAAGTT+CGG - chr1.4:74435174-74435193 MS.gene34001:intron 40.0%
AGACATTCTTTCGTTGTGTC+TGG - chr1.4:74435318-74435337 MS.gene34001:intron 40.0%
AGGAGATTAGGAACGATAAC+TGG - chr1.4:74436278-74436297 MS.gene34001:CDS 40.0%
ATGAACAACCCTAATTTCCG+AGG + chr1.4:74434254-74434273 None:intergenic 40.0%
CATTCGCAAAACGTGATGAT+GGG - chr1.4:74435049-74435068 MS.gene34001:intron 40.0%
GAGATTAGGAACGATAACTG+GGG - chr1.4:74436280-74436299 MS.gene34001:CDS 40.0%
GCTGAAGAAGCAATTTGATG+AGG - chr1.4:74436258-74436277 MS.gene34001:CDS 40.0%
GGAGATTAGGAACGATAACT+GGG - chr1.4:74436279-74436298 MS.gene34001:CDS 40.0%
TCAAATTGCTTCTTCAGCTC+AGG + chr1.4:74436256-74436275 None:intergenic 40.0%
TCAGACACACTAACAAGTGT+CGG + chr1.4:74433760-74433779 None:intergenic 40.0%
TCATTCGCAAAACGTGATGA+TGG - chr1.4:74435048-74435067 MS.gene34001:intron 40.0%
TGTGAAGGAATTTAGCTACC+TGG + chr1.4:74433298-74433317 None:intergenic 40.0%
TTTAGGGGTAAAGTGGTAGA+AGG - chr1.4:74434136-74434155 MS.gene34001:intron 40.0%
! AAAGTACTCATCTGCTGTCA+TGG + chr1.4:74436224-74436243 None:intergenic 40.0%
! AAGCCTTCAAACAGAAGTGT+TGG + chr1.4:74433728-74433747 None:intergenic 40.0%
! ATTCGCAAAACGTGATGATG+GGG - chr1.4:74435050-74435069 MS.gene34001:intron 40.0%
! TCTCTTCTTTAGGGGTAAAG+TGG - chr1.4:74434129-74434148 MS.gene34001:intron 40.0%
! TGCTTTTGCATTGCAGAACA+CGG + chr1.4:74435624-74435643 None:intergenic 40.0%
!! AATTTCAGATAGGGGTGTTC+GGG + chr1.4:74435295-74435314 None:intergenic 40.0%
!! AGTTCTAGGAAACGTTGTCA+TGG + chr1.4:74435509-74435528 None:intergenic 40.0%
!! CAATTTCAGATAGGGGTGTT+CGG + chr1.4:74435296-74435315 None:intergenic 40.0%
!! GTTCTAGGAAACGTTGTCAT+GGG + chr1.4:74435508-74435527 None:intergenic 40.0%
ACAACCCTAATTTCCGAGGA+TGG + chr1.4:74434250-74434269 None:intergenic 45.0%
ACGGATCCGAACTTGTACTT+TGG + chr1.4:74435183-74435202 None:intergenic 45.0%
ACTCGAAACAAAGTTCAGCC+AGG - chr1.4:74433277-74433296 MS.gene34001:intron 45.0%
AGAGAGAGCTGACAACAGTT+TGG + chr1.4:74434431-74434450 None:intergenic 45.0%
CAGATGTTGCATTCAAAGCC+GGG - chr1.4:74433153-74433172 MS.gene34001:CDS 45.0%
CATACACAGTGAGAGAGATG+AGG - chr1.4:74435789-74435808 MS.gene34001:intron 45.0%
TGCTAATGTACAGTCGCTGA+AGG - chr1.4:74436107-74436126 MS.gene34001:intron 45.0%
! GCTGAAGTTGGTTGAGCTTA+AGG - chr1.4:74436035-74436054 MS.gene34001:intron 45.0%
! TTTTGTGCGAACTGAACTGC+TGG - chr1.4:74434344-74434363 MS.gene34001:intron 45.0%
!! ATTACCGGTGTTGATGTGTC+AGG - chr1.4:74433826-74433845 MS.gene34001:intron 45.0%
!! ATTTCAGATAGGGGTGTTCG+GGG + chr1.4:74435294-74435313 None:intergenic 45.0%
!! CACTAACAAGTGTCGGACAT+CGG + chr1.4:74433753-74433772 None:intergenic 45.0%
!! TTTTAAGGATCGGGGCAGTT+GGG + chr1.4:74434307-74434326 None:intergenic 45.0%
!! TTTTTAAGGATCGGGGCAGT+TGG + chr1.4:74434308-74434327 None:intergenic 45.0%
!! AATAAAAAAATACTAATATA+AGG + chr1.4:74434025-74434044 None:intergenic 5.0%
!! ATAAAAAAATACTAATATAA+GGG + chr1.4:74434024-74434043 None:intergenic 5.0%
AAGCCGGGAAGAAGATCGAT+TGG - chr1.4:74433168-74433187 MS.gene34001:CDS 50.0%
AGCCGGGAAGAAGATCGATT+GGG - chr1.4:74433169-74433188 MS.gene34001:CDS 50.0%
AGGAATTGGCACTCGTTTGG+TGG - chr1.4:74433946-74433965 MS.gene34001:intron 50.0%
CTTCTTCAGCTCAGGATGCT+CGG + chr1.4:74436248-74436267 None:intergenic 50.0%
GACACCTGACACATCAACAC+CGG + chr1.4:74433833-74433852 None:intergenic 50.0%
GGAAGAAGATCGATTGGGAC+GGG - chr1.4:74433174-74433193 MS.gene34001:CDS 50.0%
GGGAAGAAGATCGATTGGGA+CGG - chr1.4:74433173-74433192 MS.gene34001:CDS 50.0%
GGTTGAGCTTAAGGAAGCAG+AGG - chr1.4:74436044-74436063 MS.gene34001:intron 50.0%
GTCCCAATCGATCTTCTTCC+CGG + chr1.4:74433174-74433193 None:intergenic 50.0%
TATTCCCAGTCTATGGGCTC+AGG + chr1.4:74433917-74433936 None:intergenic 50.0%
!! AAACGTGATGATGGGGTGGA+AGG - chr1.4:74435057-74435076 MS.gene34001:intron 50.0%
GAAGATCGATTGGGACGGGA+TGG - chr1.4:74433178-74433197 MS.gene34001:CDS 55.0%
!! CGCAAAACGTGATGATGGGG+TGG - chr1.4:74435053-74435072 MS.gene34001:intron 55.0%
AGGAGCCTGAGCCCATAGAC+TGG - chr1.4:74433909-74433928 MS.gene34001:intron 60.0%
GGAGCCTGAGCCCATAGACT+GGG - chr1.4:74433910-74433929 MS.gene34001:intron 60.0%
!! CAGATAGGGGTGTTCGGGGT+TGG + chr1.4:74435290-74435309 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74433128 74436336 74433128 ID=MS.gene34001
chr1.4 mRNA 74433128 74436336 74433128 ID=MS.gene34001.t1;Parent=MS.gene34001
chr1.4 exon 74436166 74436336 74436166 ID=MS.gene34001.t1.exon1;Parent=MS.gene34001.t1
chr1.4 CDS 74436166 74436336 74436166 ID=cds.MS.gene34001.t1;Parent=MS.gene34001.t1
chr1.4 exon 74435472 74435553 74435472 ID=MS.gene34001.t1.exon2;Parent=MS.gene34001.t1
chr1.4 CDS 74435472 74435553 74435472 ID=cds.MS.gene34001.t1;Parent=MS.gene34001.t1
chr1.4 exon 74433512 74433576 74433512 ID=MS.gene34001.t1.exon3;Parent=MS.gene34001.t1
chr1.4 CDS 74433512 74433576 74433512 ID=cds.MS.gene34001.t1;Parent=MS.gene34001.t1
chr1.4 exon 74433336 74433422 74433336 ID=MS.gene34001.t1.exon4;Parent=MS.gene34001.t1
chr1.4 CDS 74433336 74433422 74433336 ID=cds.MS.gene34001.t1;Parent=MS.gene34001.t1
chr1.4 exon 74433128 74433256 74433128 ID=MS.gene34001.t1.exon5;Parent=MS.gene34001.t1
chr1.4 CDS 74433128 74433256 74433128 ID=cds.MS.gene34001.t1;Parent=MS.gene34001.t1
Gene Sequence

>MS.gene34001

ATGAGCGGAACAGTGAAAAAAGTTACAGATGTTGCATTCAAAGCCGGGAAGAAGATCGATTGGGACGGGATGGCTAAGCTTCTCGTCACCGATGAAGCTCGTCGTGAATTTTTCAATCTTCGTCGCGCTTTCGACGAAGTTAACACTCAACTCGAAACAAAGTTCAGCCAGGTAGCTAAATTCCTTCACAGATCTCTCTAATTTCATTCTCGTTATTGTATTTAAGATTCAGATTTACCTTCATTTTGTTCTTAGATGCTAGATCGATTGACTTTTAGGTTAAAATTGTAAAACTGATTATAGATTTGAATTAGTTTAAGTCGTATTACGTTTCTCTTCATCATCAATTTTGAATTGGTTGATGATAATCATGTTTTAGTGCATTTTCGGTTTTTTTTTTTTTTTTATCAATTTATTGAGATGCTAAAAAAATTAATGTTTCTGTTGTGTAACATTTAGTTGTACACCTTTTCATCATGTTTTGAATACGAGTTTTCCTATACAAATAGATTATGTATTTAGTTACGAGGCTTAGTGGCTACTTGAATATATCTTGTGTTCGAAATGCTATACAAAATATTTTCATACTTTGAAATACCAACACTTCTGTTTGAAGGCTTGTCCGATGTCCGACACTTGTTAGTGTGTCTGACTGCTCACATACATGTAATTACATCCAATTATGTAGTCATCAAATTATTACCGGTGTTGATGTGTCAGGTGTCTGTGTATATCATTTTATGCTTCATAGCTCTTATGTATATCATTTCTTATTAATCGCAGGAGCCTGAGCCCATAGACTGGGAATATTATAGAAAAGGAATTGGCACTCGTTTGGTGGATATGTACAAGCAGCATTATGAGAGTATGTTTTTGAACTCTATTCTGAATGCCCCTTATATTAGTATTTTTTTATTTGTCAATGGTATTCATTTGTGATAGTTAGAGCATGACTTGTTTGATGGCAATTACATGATAGAATTGATTCTTGTCATGCACTTTCTCTTCTTTAGGGGTAAAGTGGTAGAAGGATATTGATAAATTCAACTGGATCCGATTCTTGTATTCAATGGTACCATATTCTTTTCATAGGGAAGAATATTATATAATTCACAAAAGCCATCCTCGGAAATTAGGGTTGTTCATATAACCAAAAAAACTGAACTGTACGGAGAACCCAACTGCCCCGATCCTTAAAAAAACTGAACTGTTTGCATTTTGTGCGAACTGAACTGCTGGATAAAAATATGATCAGTGAACTGTACCATTCTTCAAAAGTAGTTCAGTGAACTTATATAAACCAAACTGTTGTCAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTCATGCTTACAGCTCAACTAAACTTTTTACACAATGCTGACGATTTAGAAAAATTCCCTTAGCACAATTGACATCTCATACAATAAAAATGAGAAATTAATATAGGTAGACATGATGATGACGAAAAGCAGTTTTACAAACAGTTGGCTAATAGAAAATTGTTTACTTTCCAATTTTTGTTGTCAAGTTATTTATTAAAATTATTTTTTTTATTGGTATAAGAGTAGAAATAGTATAAATCCAAAATACAAATATTTACAGTAATTTTTTTTATATATATATATATTGAAAATTAATCTTTCAATTTTATGAAAACATATTTTTTTAATCTGTATTTAATGGAAGAAAAAAATATAAGCATTTATTAGTATTTTTATTTTTGTAAGAATAATTGATCAATTCTTTTTATTAGTGCGTTTAGTGAGCAACTATTCTTTATTATGTGTAAGTGTACAGTGTGTACACACAAGTCAAAAGCAACTCTACCAGCTATCAAGATTATCTTTTTGGTTGTCAAAAATAAACCAATATCTTTTTGTCATTCGCAAAACGTGATGATGGGGTGGAAGGAACTGTAGCGTGCTGCTATGTATATTTTTAAAACCCATTAGTTGCAGTTTTGCTTAGCCAGCCAGTTCAGTTATATAATTATGAACCATAGTTTACTGGACCAAAGTACAAGTTCGGATCCGTTCAAGCTATTAAGTATCAGTTCAGTTCAGTTTTATAGTTGTTTTTTTCAGGTCAGTTAGGTTCAGTGCAGTTTATATTTAGAAACCAACCCCGAACACCCCTATCTGAAATTGATAGACATTCTTTCGTTGTGTCTGGTAATTGATGTTGTTGCACTGTAATCTATAGCCTAATGCATGTTAAAATTTAACGCCAAAATTTACATTAGAATATATGAAACTGTGGATTGTGGAACTGTTCAGTTGTTATTCAATTATTTCTCAAATTGAAAAGGAAGTACATAAGAAAAGAAACTAGTCATGCCCATGACAACGTTTCCTAGAACTTTGCCTCATTCAATATATTGTTATTTGACCTATTCACATGGGAATAAAACCTGGAAATTGTATATGGTATCATGTTTGCTCGCTGATATATTCCGTGTTCTGCAATGCAAAAGCAGAGTAGTGTTCATCCTTGCATTTATTACTCGTGCATTGAATTGATTTAAGGAAAATATGATTGTTTCTTTATTGAAATTTATGCTCGAAATCATCACGTGTGTATCAGTTATTGCCTAAATATTTATGGATGTAGTCATGTAGAGCATACACAGTGAGAGAGATGAGGAGTTGATATTGACCATTCCGCCATTCAAATTTTACATACCATTAATTCAACTTTTGATGTTATGCTTCTTCTGTAGGCATTGAGATCCCCAAGTTTGTTGACACAGTAACTCCTCAATATAAGCCTAAATTTGATGCACTGGTAAGAACTTTTGTTACATATTTAGTCCAACTTTTCTCTATCAATCAAGTTACATTTTATTAAGTGATATATGTGTTTTCTTGCTGAAGTTGGTTGAGCTTAAGGAAGCAGAGGAGAAATCTCTGAAAGAATCTGAACGTTTGGAAAAGGAAATTGCTAATGTACAGTCGCTGAAGGTAATAGAAATCACCACATGGTTTCTCAGTTTTATGTAATATTGGATATTCTCTTACATACCATTGTTTGTTTTCACAGAAAAGGATTTCTACCATGACAGCAGATGAGTACTTTGCCGAGCATCCTGAGCTGAAGAAGCAATTTGATGAGGAGATTAGGAACGATAACTGGGGCTATTCACACTACAAAACTGAGCAAAACGCCTGA
Protein sequence

>MS.gene34001.t1

MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPEPIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIANVQSLKKRISTMTADEYFAEHPELKKQFDEEIRNDNWGYSHYKTEQNA