Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34159.t1 | A4ULF8.1 | 100 | 396 | 0 | 0 | 1 | 396 | 1 | 396 | 2.50E-232 | 814.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34159.t1 | A4ULF8 | 100.0 | 396 | 0 | 0 | 1 | 396 | 1 | 396 | 5.9e-235 | 814.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34159.t1 | A0A072V8Q4 | 99.5 | 396 | 2 | 0 | 1 | 396 | 1 | 396 | 2.0e-231 | 810.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28651 | MS.gene34159 | PPI |
| MS.gene28853 | MS.gene34159 | PPI |
| MS.gene072659 | MS.gene34159 | PPI |
| MS.gene050426 | MS.gene34159 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34159.t1 | MTR_2g046710 | 99.495 | 396 | 2 | 0 | 1 | 396 | 1 | 396 | 0.0 | 816 |
| MS.gene34159.t1 | MTR_4g123810 | 93.622 | 392 | 25 | 0 | 1 | 392 | 1 | 392 | 0.0 | 741 |
| MS.gene34159.t1 | MTR_7g110310 | 93.316 | 389 | 26 | 0 | 4 | 392 | 3 | 391 | 0.0 | 739 |
| MS.gene34159.t1 | MTR_7g102120 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 704 |
| MS.gene34159.t1 | MTR_1g063060 | 87.824 | 386 | 47 | 0 | 4 | 389 | 2 | 387 | 0.0 | 693 |
| MS.gene34159.t1 | MTR_1g063060 | 87.824 | 386 | 47 | 0 | 4 | 389 | 20 | 405 | 0.0 | 692 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34159.t1 | AT3G17390 | 94.103 | 390 | 23 | 0 | 4 | 393 | 2 | 391 | 0.0 | 743 |
| MS.gene34159.t1 | AT1G02500 | 92.545 | 389 | 29 | 0 | 4 | 392 | 2 | 390 | 0.0 | 724 |
| MS.gene34159.t1 | AT1G02500 | 92.545 | 389 | 29 | 0 | 4 | 392 | 2 | 390 | 0.0 | 724 |
| MS.gene34159.t1 | AT4G01850 | 91.003 | 389 | 35 | 0 | 4 | 392 | 2 | 390 | 0.0 | 721 |
| MS.gene34159.t1 | AT4G01850 | 91.003 | 389 | 35 | 0 | 4 | 392 | 2 | 390 | 0.0 | 721 |
| MS.gene34159.t1 | AT2G36880 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 707 |
| MS.gene34159.t1 | AT2G36880 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 707 |
Find 0 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGAGTATTTCAATGACAA+GGG | + | 15214:13496-13515 | MS.gene34159:CDS | 25.0% |
| ! | TATTGAGTATTTCAATGACA+AGG | + | 15214:13495-13514 | MS.gene34159:CDS | 25.0% |
| AGGTTTCTTTCACAATGTTA+AGG | - | 15214:13978-13997 | MS.gene34159:intergenic | 30.0% | |
| ATGTTAAGGATTTCCTTATC+AGG | - | 15214:13964-13983 | MS.gene34159:intergenic | 30.0% | |
| GAAATACTCAATAGTGACTT+GGG | - | 15214:13488-13507 | MS.gene34159:intergenic | 30.0% | |
| TATCAACCTTGATCTTAAGA+GGG | + | 15214:14023-14042 | MS.gene34159:CDS | 30.0% | |
| TGAAATACTCAATAGTGACT+TGG | - | 15214:13489-13508 | MS.gene34159:intergenic | 30.0% | |
| TGTTAAGGATTTCCTTATCA+GGG | - | 15214:13963-13982 | MS.gene34159:intergenic | 30.0% | |
| AAGATCATCATCGATACCTA+CGG | + | 15214:13733-13752 | MS.gene34159:CDS | 35.0% | |
| ACAAAAAGACCTGAGGAAAT+TGG | + | 15214:13310-13329 | MS.gene34159:CDS | 35.0% | |
| ACCGTAAGAATCAACAAACA+CGG | - | 15214:13929-13948 | MS.gene34159:intergenic | 35.0% | |
| ATCAACCTTGATCTTAAGAG+GGG | + | 15214:14024-14043 | MS.gene34159:CDS | 35.0% | |
| ATCAGCTGCAATTTCATCAT+TGG | - | 15214:13584-13603 | MS.gene34159:intergenic | 35.0% | |
| CTATCAACCTTGATCTTAAG+AGG | + | 15214:14022-14041 | MS.gene34159:CDS | 35.0% | |
| TAACTTGGTTGCAAGAACAT+GGG | - | 15214:13401-13420 | MS.gene34159:intergenic | 35.0% | |
| TCACAGATTCAGATGTGTAT+AGG | - | 15214:12991-13010 | MS.gene34159:intergenic | 35.0% | |
| TCTGTTGTTCAATGTTGACA+AGG | - | 15214:13255-13274 | MS.gene34159:intergenic | 35.0% | |
| ! | GACTTTTGTTGGATCTTTAC+CGG | - | 15214:13791-13810 | MS.gene34159:intergenic | 35.0% |
| ! | TGAAAGAAACCTTTGACTTC+AGG | + | 15214:13986-14005 | MS.gene34159:CDS | 35.0% |
| !! | AGATGATGCTGATTTCACAT+GGG | + | 15214:14095-14114 | MS.gene34159:CDS | 35.0% |
| !! | GCAAAGAGTATTGTTGCTAA+TGG | + | 15214:13844-13863 | MS.gene34159:CDS | 35.0% |
| !! | TCCGTGTTTGTTGATTCTTA+CGG | + | 15214:13925-13944 | MS.gene34159:CDS | 35.0% |
| !! | TTTGTTGATTCTTACGGTAC+CGG | + | 15214:13931-13950 | MS.gene34159:CDS | 35.0% |
| !! | TTTTTGAAGACTGCTGCTTA+TGG | + | 15214:14060-14079 | MS.gene34159:CDS | 35.0% |
| !!! | GGTTTTGTTTCAGATGATGT+TGG | + | 15214:13205-13224 | MS.gene34159:CDS | 35.0% |
| AACTTGCACAAAGACCAACA+TGG | + | 15214:13105-13124 | MS.gene34159:CDS | 40.0% | |
| AAGATCCAACAAAAGTCGAC+AGG | + | 15214:13794-13813 | MS.gene34159:CDS | 40.0% | |
| ACATCTGAATCTGTGAACGA+GGG | + | 15214:12995-13014 | MS.gene34159:CDS | 40.0% | |
| ACCAAAGACCATAACCATGT+TGG | - | 15214:13122-13141 | MS.gene34159:intergenic | 40.0% | |
| ACCAACATGGTTATGGTCTT+TGG | + | 15214:13118-13137 | MS.gene34159:CDS | 40.0% | |
| AGATTGTTCGTAACACATGC+AGG | + | 15214:13176-13195 | MS.gene34159:CDS | 40.0% | |
| CACAAAGACCAACATGGTTA+TGG | + | 15214:13111-13130 | MS.gene34159:CDS | 40.0% | |
| CATAAGCTCAGGTGTTTCAT+CGG | - | 15214:13371-13390 | MS.gene34159:intergenic | 40.0% | |
| CATGTTCTTGCAACCAAGTT+AGG | + | 15214:13400-13419 | MS.gene34159:CDS | 40.0% | |
| CCATCTTACAAAAAGACCTG+AGG | + | 15214:13303-13322 | MS.gene34159:CDS | 40.0% | |
| CTAACTTGGTTGCAAGAACA+TGG | - | 15214:13402-13421 | MS.gene34159:intergenic | 40.0% | |
| GAATCAACAAACACGGACAA+TGG | - | 15214:13922-13941 | MS.gene34159:intergenic | 40.0% | |
| GTGCAAGTTTCCTATGCTAT+CGG | + | 15214:13886-13905 | MS.gene34159:CDS | 40.0% | |
| TGAACACCTTGAGCAATATC+AGG | - | 15214:13280-13299 | MS.gene34159:intergenic | 40.0% | |
| TTAAGAGGGGTGGAAATAAC+AGG | + | 15214:14037-14056 | MS.gene34159:CDS | 40.0% | |
| TTCCTTATCAGGGATCTTTC+CGG | - | 15214:13953-13972 | MS.gene34159:intergenic | 40.0% | |
| ! | CCTCAGGTCTTTTTGTAAGA+TGG | - | 15214:13306-13325 | MS.gene34159:intergenic | 40.0% |
| ! | GATATTGCTCAAGGTGTTCA+TGG | + | 15214:13280-13299 | MS.gene34159:CDS | 40.0% |
| ! | GGAAGATGGTTTTCTCATCA+AGG | - | 15214:13648-13667 | MS.gene34159:intergenic | 40.0% |
| ! | GTTGCAAGAACATGGGTTAA+AGG | - | 15214:13394-13413 | MS.gene34159:intergenic | 40.0% |
| ! | TCTCATCAAGGTACTTCTCT+GGG | - | 15214:13636-13655 | MS.gene34159:intergenic | 40.0% |
| ! | TTCTCATCAAGGTACTTCTC+TGG | - | 15214:13637-13656 | MS.gene34159:intergenic | 40.0% |
| !! | ACTGCTGCTTATGGACATTT+TGG | + | 15214:14069-14088 | MS.gene34159:CDS | 40.0% |
| !! | AGGTATCGATGATGATCTTG+CGG | - | 15214:13732-13751 | MS.gene34159:intergenic | 40.0% |
| !! | AGGTTGATAGAGATCATTCC+TGG | - | 15214:14012-14031 | MS.gene34159:intergenic | 40.0% |
| !! | GAGATGATGCTGATTTCACA+TGG | + | 15214:14094-14113 | MS.gene34159:CDS | 40.0% |
| !! | TTGCTAATGGACTTGCTAGA+AGG | + | 15214:13857-13876 | MS.gene34159:CDS | 40.0% |
| AACCTTGATCTTAAGAGGGG+TGG | + | 15214:14027-14046 | MS.gene34159:CDS | 45.0% | |
| AGATAAGGACGGTATGGACA+CGG | - | 15214:13537-13556 | MS.gene34159:intergenic | 45.0% | |
| AGTGAGCTTAGCTCCTAACT+TGG | - | 15214:13416-13435 | MS.gene34159:intergenic | 45.0% | |
| ATCATCATCGATACCTACGG+TGG | + | 15214:13736-13755 | MS.gene34159:CDS | 45.0% | |
| ATCTTCCACTTGAACCCTTC+AGG | + | 15214:13661-13680 | MS.gene34159:CDS | 45.0% | |
| CACATCTGAATCTGTGAACG+AGG | + | 15214:12994-13013 | MS.gene34159:CDS | 45.0% | |
| CAGAGTCCTGATATTGCTCA+AGG | + | 15214:13271-13290 | MS.gene34159:CDS | 45.0% | |
| CGTAACACATGCAGGAACAT+AGG | + | 15214:13184-13203 | MS.gene34159:CDS | 45.0% | |
| CTCAAGGCAAGCATCAAGAA+CGG | - | 15214:13056-13075 | MS.gene34159:intergenic | 45.0% | |
| CTCACTGAAGTTCGCAAGAA+CGG | + | 15214:13430-13449 | MS.gene34159:CDS | 45.0% | |
| GAAGTTGTGAAGCCACTCAA+GGG | + | 15214:14117-14136 | MS.gene34159:CDS | 45.0% | |
| GGTGATCAAGGTCACATGTT+CGG | + | 15214:13337-13356 | MS.gene34159:CDS | 45.0% | |
| GTGATCAAGGTCACATGTTC+GGG | + | 15214:13338-13357 | MS.gene34159:CDS | 45.0% | |
| GTGCTGTGTTGAGATAAGGA+CGG | - | 15214:13548-13567 | MS.gene34159:intergenic | 45.0% | |
| GTGTTGAGATAAGGACGGTA+TGG | - | 15214:13543-13562 | MS.gene34159:intergenic | 45.0% | |
| TACCGGAAAGATCCCTGATA+AGG | + | 15214:13948-13967 | MS.gene34159:CDS | 45.0% | |
| TGAAGGGTTCAAGTGGAAGA+TGG | - | 15214:13662-13681 | MS.gene34159:intergenic | 45.0% | |
| TTCCACCCCTCTTAAGATCA+AGG | - | 15214:14032-14051 | MS.gene34159:intergenic | 45.0% | |
| TTTCCATCAGGTCTCAACCA+AGG | - | 15214:13463-13482 | MS.gene34159:intergenic | 45.0% | |
| ! | TCACATGCAACTTTGCTGTC+TGG | - | 15214:13085-13104 | MS.gene34159:intergenic | 45.0% |
| ! | TGGGTTAAAGGCATAAGCTC+AGG | - | 15214:13382-13401 | MS.gene34159:intergenic | 45.0% |
| !! | GAAATTGGTGCTGGTGATCA+AGG | + | 15214:13325-13344 | MS.gene34159:CDS | 45.0% |
| !! | TTTCAATGACAAGGGTGCCA+TGG | + | 15214:13504-13523 | MS.gene34159:CDS | 45.0% |
| ACAAACACGGACAATGGCTC+TGG | - | 15214:13916-13935 | MS.gene34159:intergenic | 50.0% | |
| AGACCTGAGGAAATTGGTGC+TGG | + | 15214:13316-13335 | MS.gene34159:CDS | 50.0% | |
| ATCGATACCTACGGTGGTTG+GGG | + | 15214:13742-13761 | MS.gene34159:CDS | 50.0% | |
| ATCGATGATGATCTTGCGGC+CGG | - | 15214:13728-13747 | MS.gene34159:intergenic | 50.0% | |
| ATCTGGTCGCAAAGCTTGTC+GGG | - | 15214:13025-13044 | MS.gene34159:intergenic | 50.0% | |
| CAAGAACGGCATCAGAGATC+TGG | - | 15214:13042-13061 | MS.gene34159:intergenic | 50.0% | |
| CATCGATACCTACGGTGGTT+GGG | + | 15214:13741-13760 | MS.gene34159:CDS | 50.0% | |
| CATTCCTGGCCTGAAGTCAA+AGG | - | 15214:13998-14017 | MS.gene34159:intergenic | 50.0% | |
| CCAACAAAAGTCGACAGGAG+TGG | + | 15214:13799-13818 | MS.gene34159:CDS | 50.0% | |
| CGTCGTGCTGTGTTGAGATA+AGG | - | 15214:13552-13571 | MS.gene34159:intergenic | 50.0% | |
| GATCTGGTCGCAAAGCTTGT+CGG | - | 15214:13026-13045 | MS.gene34159:intergenic | 50.0% | |
| GCAAGAACGGAACATGTCCT+TGG | + | 15214:13443-13462 | MS.gene34159:CDS | 50.0% | |
| GCTAACTTTCCACCCTTGAG+TGG | - | 15214:14132-14151 | MS.gene34159:intergenic | 50.0% | |
| GGAAGTTGTGAAGCCACTCA+AGG | + | 15214:14116-14135 | MS.gene34159:CDS | 50.0% | |
| TCACCAGCACCAATTTCCTC+AGG | - | 15214:13322-13341 | MS.gene34159:intergenic | 50.0% | |
| TCATCGATACCTACGGTGGT+TGG | + | 15214:13740-13759 | MS.gene34159:CDS | 50.0% | |
| TGCTGTCTGGATCTTGCTCA+AGG | - | 15214:13072-13091 | MS.gene34159:intergenic | 50.0% | |
| TGTCCTTGGTTGAGACCTGA+TGG | + | 15214:13457-13476 | MS.gene34159:CDS | 50.0% | |
| ! | GAAACCTTTGACTTCAGGCC+AGG | + | 15214:13991-14010 | MS.gene34159:CDS | 50.0% |
| ! | GTGACTTGGGTCTTTCCATC+AGG | - | 15214:13475-13494 | MS.gene34159:intergenic | 50.0% |
| !! | AGGTACTTCTCTGGGATCAC+AGG | - | 15214:13628-13647 | MS.gene34159:intergenic | 50.0% |
| !! | CTCTGGAACACCGATAGCAT+AGG | - | 15214:13899-13918 | MS.gene34159:intergenic | 50.0% |
| !!! | CCACTCCTGTCGACTTTTGT+TGG | - | 15214:13802-13821 | MS.gene34159:intergenic | 50.0% |
| AACGGCCTGAAGGGTTCAAG+TGG | - | 15214:13669-13688 | MS.gene34159:intergenic | 55.0% | |
| AGGACGGTATGGACACGGAT+CGG | - | 15214:13532-13551 | MS.gene34159:intergenic | 55.0% | |
| ATGGACACGGATCGGAACCA+TGG | - | 15214:13524-13543 | MS.gene34159:intergenic | 55.0% | |
| CCTTCAGGCCGTTTCGTCAT+CGG | + | 15214:13676-13695 | MS.gene34159:CDS | 55.0% | |
| CGATGACGAAACGGCCTGAA+GGG | - | 15214:13678-13697 | MS.gene34159:intergenic | 55.0% | |
| GAGGACCACCGATGACGAAA+CGG | - | 15214:13687-13706 | MS.gene34159:intergenic | 55.0% | |
| GTTGTGAAGCCACTCAAGGG+TGG | + | 15214:14120-14139 | MS.gene34159:CDS | 55.0% | |
| TCTGGTCGCAAAGCTTGTCG+GGG | - | 15214:13024-13043 | MS.gene34159:intergenic | 55.0% | |
| TTCGTCATCGGTGGTCCTCA+CGG | + | 15214:13688-13707 | MS.gene34159:CDS | 55.0% | |
| CCGATGACGAAACGGCCTGA+AGG | - | 15214:13679-13698 | MS.gene34159:intergenic | 60.0% | |
| GTTAGACCAGCGTCACCGTG+AGG | - | 15214:13706-13725 | MS.gene34159:intergenic | 60.0% | |
| TACGGTGGTTGGGGTGCACA+CGG | + | 15214:13751-13770 | MS.gene34159:CDS | 60.0% | |
| TCAGGCCGTTTCGTCATCGG+TGG | + | 15214:13679-13698 | MS.gene34159:CDS | 60.0% | |
| ! | CACGGTGACGCTGGTCTAAC+CGG | + | 15214:13706-13725 | MS.gene34159:CDS | 60.0% |
| ! | CACGGTGGTGGTGCTTTCTC+CGG | + | 15214:13769-13788 | MS.gene34159:CDS | 60.0% |
| GTGTGCACCCCAACCACCGT+AGG | - | 15214:13752-13771 | MS.gene34159:intergenic | 65.0% | |
| GGTGGTCCTCACGGTGACGC+TGG | + | 15214:13697-13716 | MS.gene34159:CDS | 70.0% | |
| GGTGGTTGGGGTGCACACGG+TGG | + | 15214:13754-13773 | MS.gene34159:CDS | 70.0% | |
| GGTTGGGGTGCACACGGTGG+TGG | + | 15214:13757-13776 | MS.gene34159:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15214 | gene | 12971 | 14161 | 12971 | ID=MS.gene34159 |
| 15214 | mRNA | 12971 | 14161 | 12971 | ID=MS.gene34159.t1;Parent=MS.gene34159 |
| 15214 | exon | 12971 | 14161 | 12971 | ID=MS.gene34159.t1.exon1;Parent=MS.gene34159.t1 |
| 15214 | CDS | 12971 | 14161 | 12971 | ID=cds.MS.gene34159.t1;Parent=MS.gene34159.t1 |
| Gene Sequence |
| Protein sequence |
