AlfalfaGEDB | MS.gene34301

M. sativa cultivar XinJiangDaYe / MS.gene34301

KEGG: -
GO: GO:0005575 GO:0016020 GO:0110165
PFAM: PF02325.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34301.t1	XP_013454503.1	80.3	228	30	4	1	227	1	214	3.60E-79	304.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34301.t1	Q9SUE0	67.5	120	35	1	104	223	98	213	3.2e-39	163.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34301.t1	A0A072UFK1	80.3	228	30	4	1	227	1	214	2.6e-79	304.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050163	MS.gene34301	0.801361	9.34E-49	-1.69E-46
MS.gene050165	MS.gene34301	0.810027	1.41E-50	-1.69E-46
MS.gene050333	MS.gene34301	0.821267	4.42E-53	-1.69E-46
MS.gene050444	MS.gene34301	0.805142	1.54E-49	-1.69E-46
MS.gene053284	MS.gene34301	0.882746	8.04E-71	-1.69E-46
MS.gene053285	MS.gene34301	0.884772	1.44E-71	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34301.t1	MTR_4g005520	86.957	207	14	4	21	227	21	214	2.13e-120	341
MS.gene34301.t1	MTR_3g105100	47.368	76	35	3	145	219	120	191	3.61e-13	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34301.t1	AT4G27990	62.329	146	47	3	84	223	70	213	3.51e-55	176
MS.gene34301.t1	AT3G07430	52.695	167	61	2	70	223	66	227	8.65e-51	165
MS.gene34301.t1	AT5G21920	50.000	76	33	3	145	219	133	204	1.06e-13	68.6

Find 67 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGGTTTAGGTTTGGGTATG+GGG	0.273496	4.3:+88950063	MS.gene34301:CDS
GACAACCATTGTCTGCAATT+AGG	0.283838	4.3:+88950561	MS.gene34301:CDS
GCAACGATGGGTTTAGGTTT+AGG	0.287429	4.3:+88950050	MS.gene34301:CDS
TGAGGGATGATTAGGTTATT+AGG	0.296692	4.3:-88950140	None:intergenic
ACAACCATTGTCTGCAATTA+GGG	0.307081	4.3:+88950562	MS.gene34301:CDS
TTAGGTTTAGGTTTGGGTAT+GGG	0.307229	4.3:+88950062	MS.gene34301:CDS
ATAAAAGCGCGGTGGCGAAT+AGG	0.322008	4.3:-88950310	None:intergenic
ACAGTGGTGTGTTGTTGGTT+AGG	0.327645	4.3:+88950501	MS.gene34301:CDS
ATCCTTATCTAAGTTTGTTT+AGG	0.329465	4.3:+88950594	MS.gene34301:CDS
GCAGCAGCAACGATGGGTTT+AGG	0.338503	4.3:+88950044	MS.gene34301:CDS
GGGTTTGAGCGGTGCGGCGG+GGG	0.348622	4.3:-88950219	None:intergenic
AGGTTTAGGTTTGGGTATGG+GGG	0.355862	4.3:+88950064	MS.gene34301:CDS
TAAAAGCGCGGTGGCGAATA+GGG	0.356707	4.3:-88950309	None:intergenic
CAGTGGTGTGTTGTTGGTTA+GGG	0.363509	4.3:+88950502	MS.gene34301:CDS
AGGGGTTTGAGCGGTGCGGC+GGG	0.370071	4.3:-88950221	None:intergenic
GACGAGATGAAGGTGAGAAA+TGG	0.370719	4.3:-88950103	None:intergenic
ACGAGATGAAGGTGAGAAAT+GGG	0.378393	4.3:-88950102	None:intergenic
GAGGGGTTTGAGCGGTGCGG+CGG	0.379926	4.3:-88950222	None:intergenic
TTCGGCGTTGTTTGTGTTGA+GGG	0.382973	4.3:-88950157	None:intergenic
ATGGGTTTAGGTTTAGGTTT+GGG	0.383576	4.3:+88950056	MS.gene34301:CDS
GGGGTTTGAGCGGTGCGGCG+GGG	0.384619	4.3:-88950220	None:intergenic
TAGCGGCGGGGTTGAAGAAA+TGG	0.403666	4.3:+88950468	MS.gene34301:CDS
GGATGGGATGGGATCTAAAC+AGG	0.408629	4.3:-88950707	None:intergenic
TTTAGGTTTAGGTTTGGGTA+TGG	0.411658	4.3:+88950061	MS.gene34301:CDS
TATTTACAGTGGTGTGTTGT+TGG	0.416695	4.3:+88950496	MS.gene34301:CDS
AACAACAACGTTCTTCTCTC+TGG	0.424828	4.3:+88950266	MS.gene34301:CDS
GTTTGTGTTGAGGGATGATT+AGG	0.430112	4.3:-88950148	None:intergenic
GACGGTGAACACGCCGTTGA+CGG	0.439588	4.3:+88950442	MS.gene34301:CDS
CCGTTGACGGTTGTAGCGGC+GGG	0.441420	4.3:+88950455	MS.gene34301:CDS
GATGGGTTTAGGTTTAGGTT+TGG	0.452940	4.3:+88950055	MS.gene34301:CDS
TGTCTTTCCCATGGAATGTT+GGG	0.454889	4.3:-88950542	None:intergenic
GGATGATTAGGTTATTAGGT+TGG	0.464532	4.3:-88950136	None:intergenic
AAAGAGAGAAGACGAGATGA+AGG	0.465410	4.3:-88950113	None:intergenic
GGTTTAGGTTTGGGTATGGG+GGG	0.472187	4.3:+88950065	MS.gene34301:CDS
TAGTCGAGAAATGGCTTCAA+TGG	0.485842	4.3:+88950022	None:intergenic
GCAATTGATACACAAAGGTC+CGG	0.490961	4.3:-88950365	None:intergenic
TTGAAATAGAACAAGTGGTT+CGG	0.503623	4.3:-88950175	None:intergenic
GTTGGTTTCCCAACATTCCA+TGG	0.512936	4.3:+88950534	MS.gene34301:CDS
GACAATGGTTGTCTTTCCCA+TGG	0.515980	4.3:-88950551	None:intergenic
GTTGCTGTCATCGTTGATGA+GGG	0.521038	4.3:-88950240	None:intergenic
TTCCTAAACAAACTTAGATA+AGG	0.528049	4.3:-88950596	None:intergenic
TTCAATGGCAGCAGCAACGA+TGG	0.533900	4.3:+88950037	MS.gene34301:CDS
GCCGTTGACGGTTGTAGCGG+CGG	0.535668	4.3:+88950454	MS.gene34301:CDS
CGTCAACATTCTTCCACCAC+CGG	0.541309	4.3:+88950346	MS.gene34301:CDS
TTGGTTTCCCAACATTCCAT+GGG	0.548737	4.3:+88950535	MS.gene34301:CDS
GTTCGGCGTTGTTTGTGTTG+AGG	0.552204	4.3:-88950158	None:intergenic
CCCGCCGCTACAACCGTCAA+CGG	0.573983	4.3:-88950455	None:intergenic
TTGTCTTTCCCATGGAATGT+TGG	0.574451	4.3:-88950543	None:intergenic
AAAACAGCAAAAGCAAGAAG+AGG	0.574512	4.3:-88950656	None:intergenic
CACGCCGTTGACGGTTGTAG+CGG	0.575214	4.3:+88950451	MS.gene34301:CDS
TCAATGGCAGCAGCAACGAT+GGG	0.589780	4.3:+88950038	MS.gene34301:CDS
TGTTGCTGTCATCGTTGATG+AGG	0.595419	4.3:-88950241	None:intergenic
GATACACAAAGGTCCGGTGG+TGG	0.605205	4.3:-88950359	None:intergenic
CGTTGACGGTTGTAGCGGCG+GGG	0.610796	4.3:+88950456	MS.gene34301:CDS
TAGGGTTGTAACGGTTCGAG+TGG	0.613197	4.3:-88950291	None:intergenic
GGCGGGGTTGAAGAAATGGT+TGG	0.623671	4.3:+88950472	MS.gene34301:CDS
GTTGATTGAAATAGAACAAG+TGG	0.628922	4.3:-88950180	None:intergenic
AAATGGTTGGATATTTACAG+TGG	0.642279	4.3:+88950485	MS.gene34301:CDS
TTGCTGTCATCGTTGATGAG+GGG	0.644652	4.3:-88950239	None:intergenic
GGTGGCGAATAGGGTTGTAA+CGG	0.659977	4.3:-88950300	None:intergenic
AGATCCCTAATTGCAGACAA+TGG	0.662621	4.3:-88950566	None:intergenic
TCGTTGATGAGGGGTTTGAG+CGG	0.662679	4.3:-88950230	None:intergenic
GATGAGGGGTTTGAGCGGTG+CGG	0.676789	4.3:-88950225	None:intergenic
TGCTTTGACACATAAAAGCG+CGG	0.687821	4.3:-88950321	None:intergenic
ATTGATACACAAAGGTCCGG+TGG	0.691756	4.3:-88950362	None:intergenic
GGATCTAAACAGGAACAGTG+AGG	0.722011	4.3:-88950697	None:intergenic
TTTGACACATAAAAGCGCGG+TGG	0.754274	4.3:-88950318	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTTATATCATGAAAAAGACT+AGG	+	chr4.3:88950414-88950433	MS.gene34301:CDS	25.0%
!	TTCCTAAACAAACTTAGATA+AGG	-	chr4.3:88950599-88950618	None:intergenic	25.0%
!!	ATCCTTATCTAAGTTTGTTT+AGG	+	chr4.3:88950594-88950613	MS.gene34301:CDS	25.0%
!!	ATTTTGCAATTGATACACAA+AGG	-	chr4.3:88950373-88950392	None:intergenic	25.0%
!!!	ATATCAAGAGTATCAAAAAC+AGG	-	chr4.3:88950632-88950651	None:intergenic	25.0%
	AAATGGTTGGATATTTACAG+TGG	+	chr4.3:88950485-88950504	MS.gene34301:CDS	30.0%
	GTTGATTGAAATAGAACAAG+TGG	-	chr4.3:88950183-88950202	None:intergenic	30.0%
	TATCATGAAAAAGACTAGGA+CGG	+	chr4.3:88950418-88950437	MS.gene34301:CDS	30.0%
	TTGAAATAGAACAAGTGGTT+CGG	-	chr4.3:88950178-88950197	None:intergenic	30.0%
	AAAACAGCAAAAGCAAGAAG+AGG	-	chr4.3:88950659-88950678	None:intergenic	35.0%
	ACAACCATTGTCTGCAATTA+GGG	+	chr4.3:88950562-88950581	MS.gene34301:CDS	35.0%
	ATCATGAAAAAGACTAGGAC+GGG	+	chr4.3:88950419-88950438	MS.gene34301:CDS	35.0%
	GGATGATTAGGTTATTAGGT+TGG	-	chr4.3:88950139-88950158	None:intergenic	35.0%
	TATTTACAGTGGTGTGTTGT+TGG	+	chr4.3:88950496-88950515	MS.gene34301:CDS	35.0%
	TGAGGGATGATTAGGTTATT+AGG	-	chr4.3:88950143-88950162	None:intergenic	35.0%
!	ATGGGTTTAGGTTTAGGTTT+GGG	+	chr4.3:88950056-88950075	MS.gene34301:CDS	35.0%
!	TTAGGTTTAGGTTTGGGTAT+GGG	+	chr4.3:88950062-88950081	MS.gene34301:CDS	35.0%
!	TTTAGGTTTAGGTTTGGGTA+TGG	+	chr4.3:88950061-88950080	MS.gene34301:CDS	35.0%
!!	TCAAGAGTATCAAAAACAGG+TGG	-	chr4.3:88950629-88950648	None:intergenic	35.0%
!!!	CTTCTTGCTTTTGCTGTTTT+GGG	+	chr4.3:88950659-88950678	MS.gene34301:CDS	35.0%
!!!	TCTTCTTGCTTTTGCTGTTT+TGG	+	chr4.3:88950658-88950677	MS.gene34301:CDS	35.0%
	AAAGAGAGAAGACGAGATGA+AGG	-	chr4.3:88950116-88950135	None:intergenic	40.0%
	AACAACAACGTTCTTCTCTC+TGG	+	chr4.3:88950266-88950285	MS.gene34301:CDS	40.0%
	ACGAGATGAAGGTGAGAAAT+GGG	-	chr4.3:88950105-88950124	None:intergenic	40.0%
	AGATCCCTAATTGCAGACAA+TGG	-	chr4.3:88950569-88950588	None:intergenic	40.0%
	GACAACCATTGTCTGCAATT+AGG	+	chr4.3:88950561-88950580	MS.gene34301:CDS	40.0%
	GCAATTGATACACAAAGGTC+CGG	-	chr4.3:88950368-88950387	None:intergenic	40.0%
	GTTTGTGTTGAGGGATGATT+AGG	-	chr4.3:88950151-88950170	None:intergenic	40.0%
	TCATGAAAAAGACTAGGACG+GGG	+	chr4.3:88950420-88950439	MS.gene34301:CDS	40.0%
	TGCTTTGACACATAAAAGCG+CGG	-	chr4.3:88950324-88950343	None:intergenic	40.0%
	TGTCTTTCCCATGGAATGTT+GGG	-	chr4.3:88950545-88950564	None:intergenic	40.0%
	TTGGTTTCCCAACATTCCAT+GGG	+	chr4.3:88950535-88950554	MS.gene34301:CDS	40.0%
	TTGTCTTTCCCATGGAATGT+TGG	-	chr4.3:88950546-88950565	None:intergenic	40.0%
!	GATGGGTTTAGGTTTAGGTT+TGG	+	chr4.3:88950055-88950074	MS.gene34301:CDS	40.0%
!	TAGGTTTAGGTTTGGGTATG+GGG	+	chr4.3:88950063-88950082	MS.gene34301:CDS	40.0%
!!!	TGGTTAGGGTTTTGTTGAGT+TGG	+	chr4.3:88950516-88950535	MS.gene34301:CDS	40.0%
	ATTGATACACAAAGGTCCGG+TGG	-	chr4.3:88950365-88950384	None:intergenic	45.0%
	GACAATGGTTGTCTTTCCCA+TGG	-	chr4.3:88950554-88950573	None:intergenic	45.0%
	GACGAGATGAAGGTGAGAAA+TGG	-	chr4.3:88950106-88950125	None:intergenic	45.0%
	TGTTGCTGTCATCGTTGATG+AGG	-	chr4.3:88950244-88950263	None:intergenic	45.0%
	TTCGGCGTTGTTTGTGTTGA+GGG	-	chr4.3:88950160-88950179	None:intergenic	45.0%
!	ACAGTGGTGTGTTGTTGGTT+AGG	+	chr4.3:88950501-88950520	MS.gene34301:CDS	45.0%
!	AGGTTTAGGTTTGGGTATGG+GGG	+	chr4.3:88950064-88950083	MS.gene34301:CDS	45.0%
!	CAGTGGTGTGTTGTTGGTTA+GGG	+	chr4.3:88950502-88950521	MS.gene34301:CDS	45.0%
!	GCAACGATGGGTTTAGGTTT+AGG	+	chr4.3:88950050-88950069	MS.gene34301:CDS	45.0%
!	GTTGCTGTCATCGTTGATGA+GGG	-	chr4.3:88950243-88950262	None:intergenic	45.0%
!	GTTGGTTTCCCAACATTCCA+TGG	+	chr4.3:88950534-88950553	MS.gene34301:CDS	45.0%
!	TTGCTGTCATCGTTGATGAG+GGG	-	chr4.3:88950242-88950261	None:intergenic	45.0%
!	TTTGACACATAAAAGCGCGG+TGG	-	chr4.3:88950321-88950340	None:intergenic	45.0%
	CGTCAACATTCTTCCACCAC+CGG	+	chr4.3:88950346-88950365	MS.gene34301:CDS	50.0%
	GAAAAAGACTAGGACGGGGA+CGG	+	chr4.3:88950424-88950443	MS.gene34301:CDS	50.0%
	GGTGGCGAATAGGGTTGTAA+CGG	-	chr4.3:88950303-88950322	None:intergenic	50.0%
	GTTCGGCGTTGTTTGTGTTG+AGG	-	chr4.3:88950161-88950180	None:intergenic	50.0%
	TAGGGTTGTAACGGTTCGAG+TGG	-	chr4.3:88950294-88950313	None:intergenic	50.0%
	TCAATGGCAGCAGCAACGAT+GGG	+	chr4.3:88950038-88950057	MS.gene34301:CDS	50.0%
	TTCAATGGCAGCAGCAACGA+TGG	+	chr4.3:88950037-88950056	MS.gene34301:CDS	50.0%
!	ATAAAAGCGCGGTGGCGAAT+AGG	-	chr4.3:88950313-88950332	None:intergenic	50.0%
!	GGTTTAGGTTTGGGTATGGG+GGG	+	chr4.3:88950065-88950084	MS.gene34301:CDS	50.0%
!	TAAAAGCGCGGTGGCGAATA+GGG	-	chr4.3:88950312-88950331	None:intergenic	50.0%
!	TCGTTGATGAGGGGTTTGAG+CGG	-	chr4.3:88950233-88950252	None:intergenic	50.0%
!!	GCTGTTTTGGGTACTCTTGC+TGG	+	chr4.3:88950671-88950690	MS.gene34301:CDS	50.0%
	GATACACAAAGGTCCGGTGG+TGG	-	chr4.3:88950362-88950381	None:intergenic	55.0%
	GGCGGGGTTGAAGAAATGGT+TGG	+	chr4.3:88950472-88950491	MS.gene34301:CDS	55.0%
	TAGCGGCGGGGTTGAAGAAA+TGG	+	chr4.3:88950468-88950487	MS.gene34301:CDS	55.0%
!	GCAGCAGCAACGATGGGTTT+AGG	+	chr4.3:88950044-88950063	MS.gene34301:CDS	55.0%
	CACGCCGTTGACGGTTGTAG+CGG	+	chr4.3:88950451-88950470	MS.gene34301:CDS	60.0%
	GACGGTGAACACGCCGTTGA+CGG	+	chr4.3:88950442-88950461	MS.gene34301:CDS	60.0%
	GATGAGGGGTTTGAGCGGTG+CGG	-	chr4.3:88950228-88950247	None:intergenic	60.0%
!!	CGGGGGTGAGGGTAGTTTTG+TGG	-	chr4.3:88950205-88950224	None:intergenic	60.0%
	CCCGCCGCTACAACCGTCAA+CGG	-	chr4.3:88950458-88950477	None:intergenic	65.0%
	CCGTTGACGGTTGTAGCGGC+GGG	+	chr4.3:88950455-88950474	MS.gene34301:CDS	65.0%
	CGTTGACGGTTGTAGCGGCG+GGG	+	chr4.3:88950456-88950475	MS.gene34301:CDS	65.0%
	GCCGTTGACGGTTGTAGCGG+CGG	+	chr4.3:88950454-88950473	MS.gene34301:CDS	65.0%
	AGGGGTTTGAGCGGTGCGGC+GGG	-	chr4.3:88950224-88950243	None:intergenic	70.0%
	GAGGGGTTTGAGCGGTGCGG+CGG	-	chr4.3:88950225-88950244	None:intergenic	70.0%
	GGGGTTTGAGCGGTGCGGCG+GGG	-	chr4.3:88950223-88950242	None:intergenic	75.0%
	GGGTTTGAGCGGTGCGGCGG+GGG	-	chr4.3:88950222-88950241	None:intergenic	75.0%
!	GAGCGGTGCGGCGGGGGTGA+GGG	-	chr4.3:88950216-88950235	None:intergenic	80.0%
!	TGAGCGGTGCGGCGGGGGTG+AGG	-	chr4.3:88950217-88950236	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	88950032	88950715	88950032	ID=MS.gene34301
chr4.3	mRNA	88950032	88950715	88950032	ID=MS.gene34301.t1;Parent=MS.gene34301
chr4.3	exon	88950032	88950715	88950032	ID=MS.gene34301.t1.exon1;Parent=MS.gene34301.t1
chr4.3	CDS	88950032	88950715	88950032	ID=cds.MS.gene34301.t1;Parent=MS.gene34301.t1

Gene Sequence

>MS.gene34301

ATGGCTTCAATGGCAGCAGCAACGATGGGTTTAGGTTTAGGTTTGGGTATGGGGGGTATGACTGTAACTTCCCATTTCTCACCTTCATCTCGTCTTCTCTCTTTCCAACCTAATAACCTAATCATCCCTCAACACAAACAACGCCGAACCACTTGTTCTATTTCAATCAACCACAAAACTACCCTCACCCCCGCCGCACCGCTCAAACCCCTCATCAACGATGACAGCAACAACAACAACAACGTTCTTCTCTCTGGTTCCACTCGAACCGTTACAACCCTATTCGCCACCGCGCTTTTATGTGTCAAAGCATTCGTCAACATTCTTCCACCACCGGACCTTTGTGTATCAATTGCAAAATCATCTTCGTCGCTTTGTTTTGCTTATATCATGAAAAAGACTAGGACGGGGACGGTGAACACGCCGTTGACGGTTGTAGCGGCGGGGTTGAAGAAATGGTTGGATATTTACAGTGGTGTGTTGTTGGTTAGGGTTTTGTTGAGTTGGTTTCCCAACATTCCATGGGAAAGACAACCATTGTCTGCAATTAGGGATCTTTGTGATCCTTATCTAAGTTTGTTTAGGAATGTTGTTCCACCTGTTTTTGATACTCTTGATATTAGTCCTCTTCTTGCTTTTGCTGTTTTGGGTACTCTTGCTGGTCTCCTCACTGTTCCTGTTTAG

Protein sequence

>MS.gene34301.t1

MASMAAATMGLGLGLGMGGMTVTSHFSPSSRLLSFQPNNLIIPQHKQRRTTCSISINHKTTLTPAAPLKPLINDDSNNNNNVLLSGSTRTVTTLFATALLCVKAFVNILPPPDLCVSIAKSSSSLCFAYIMKKTRTGTVNTPLTVVAAGLKKWLDIYSGVLLVRVLLSWFPNIPWERQPLSAIRDLCDPYLSLFRNVVPPVFDTLDISPLLAFAVLGTLAGLLTVPV