AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053285


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053285.t1 MTR_4g005520 95.745 94 4 0 1 94 121 214 1.64e-60 183
MS.gene053285.t1 MTR_3g105100 47.368 76 35 3 12 86 120 191 4.60e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053285.t1 AT4G27990 85.227 88 13 0 4 91 127 214 1.25e-51 161
MS.gene053285.t1 AT3G07430 83.908 87 14 0 4 90 141 227 9.49e-51 159
MS.gene053285.t1 AT5G21920 50.000 76 33 3 12 86 133 204 1.41e-14 66.6

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAACCATTGTCTGCAATT+AGG 0.283838 4.3:+88913347 MS.gene053285:CDS
ACAACCATTGTCTGCAATTA+GGG 0.307081 4.3:+88913348 MS.gene053285:CDS
ACAGTGGTGTGTTGTTGGTT+AGG 0.327645 4.3:+88913287 MS.gene053285:CDS
ATCCTTATCTAAGTTTGTTT+AGG 0.329465 4.3:+88913380 MS.gene053285:CDS
CAGTGGTGTGTTGTTGGTTA+GGG 0.363509 4.3:+88913288 MS.gene053285:CDS
TAGCGGCGGGGTTGAAGAAA+TGG 0.403666 4.3:+88913254 MS.gene053285:CDS
GGATGGGATGGGATCTAAAC+AGG 0.408629 4.3:-88913493 None:intergenic
TATTTACAGTGGTGTGTTGT+TGG 0.416695 4.3:+88913282 MS.gene053285:CDS
GACGGTGAACACGCCGTTGA+CGG 0.439588 4.3:+88913228 MS.gene053285:CDS
CCGTTGACGGTTGTAGCGGC+GGG 0.441420 4.3:+88913241 MS.gene053285:CDS
TGTCTTTCCCATGGAATGTT+GGG 0.454889 4.3:-88913328 None:intergenic
GTTGGTTTCCCAACATTCCA+TGG 0.512936 4.3:+88913320 MS.gene053285:CDS
GACAATGGTTGTCTTTCCCA+TGG 0.515980 4.3:-88913337 None:intergenic
TTCCTAAACAAACTTAGATA+AGG 0.528049 4.3:-88913382 None:intergenic
GCCGTTGACGGTTGTAGCGG+CGG 0.535668 4.3:+88913240 MS.gene053285:CDS
TTGGTTTCCCAACATTCCAT+GGG 0.548737 4.3:+88913321 MS.gene053285:CDS
CCCGCCGCTACAACCGTCAA+CGG 0.573983 4.3:-88913241 None:intergenic
TTGTCTTTCCCATGGAATGT+TGG 0.574451 4.3:-88913329 None:intergenic
AAAACAGCAAAAGCAAGAAG+AGG 0.574512 4.3:-88913442 None:intergenic
CACGCCGTTGACGGTTGTAG+CGG 0.575214 4.3:+88913237 MS.gene053285:CDS
CGTTGACGGTTGTAGCGGCG+GGG 0.610796 4.3:+88913242 MS.gene053285:CDS
GGCGGGGTTGAAGAAATGGT+TGG 0.623671 4.3:+88913258 MS.gene053285:CDS
AAATGGTTGGATATTTACAG+TGG 0.642279 4.3:+88913271 MS.gene053285:CDS
AGATCCCTAATTGCAGACAA+TGG 0.662621 4.3:-88913352 None:intergenic
GGATCTAAACAGGAACAGTG+AGG 0.722011 4.3:-88913483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCCTAAACAAACTTAGATA+AGG - chr4.3:88913385-88913404 None:intergenic 25.0%
!! ATCCTTATCTAAGTTTGTTT+AGG + chr4.3:88913380-88913399 MS.gene053285:CDS 25.0%
!!! ATATCAAGAGTATCAAAAAC+AGG - chr4.3:88913418-88913437 None:intergenic 25.0%
AAATGGTTGGATATTTACAG+TGG + chr4.3:88913271-88913290 MS.gene053285:CDS 30.0%
AAAACAGCAAAAGCAAGAAG+AGG - chr4.3:88913445-88913464 None:intergenic 35.0%
ACAACCATTGTCTGCAATTA+GGG + chr4.3:88913348-88913367 MS.gene053285:CDS 35.0%
TATTTACAGTGGTGTGTTGT+TGG + chr4.3:88913282-88913301 MS.gene053285:CDS 35.0%
!! TCAAGAGTATCAAAAACAGG+TGG - chr4.3:88913415-88913434 None:intergenic 35.0%
!!! CTTCTTGCTTTTGCTGTTTT+GGG + chr4.3:88913445-88913464 MS.gene053285:CDS 35.0%
!!! TCTTCTTGCTTTTGCTGTTT+TGG + chr4.3:88913444-88913463 MS.gene053285:CDS 35.0%
AGATCCCTAATTGCAGACAA+TGG - chr4.3:88913355-88913374 None:intergenic 40.0%
GACAACCATTGTCTGCAATT+AGG + chr4.3:88913347-88913366 MS.gene053285:CDS 40.0%
TGTCTTTCCCATGGAATGTT+GGG - chr4.3:88913331-88913350 None:intergenic 40.0%
TTGGTTTCCCAACATTCCAT+GGG + chr4.3:88913321-88913340 MS.gene053285:CDS 40.0%
TTGTCTTTCCCATGGAATGT+TGG - chr4.3:88913332-88913351 None:intergenic 40.0%
!!! TGGTTAGGGTTTTGTTGAGT+TGG + chr4.3:88913302-88913321 MS.gene053285:CDS 40.0%
GACAATGGTTGTCTTTCCCA+TGG - chr4.3:88913340-88913359 None:intergenic 45.0%
! ACAGTGGTGTGTTGTTGGTT+AGG + chr4.3:88913287-88913306 MS.gene053285:CDS 45.0%
! CAGTGGTGTGTTGTTGGTTA+GGG + chr4.3:88913288-88913307 MS.gene053285:CDS 45.0%
! GTTGGTTTCCCAACATTCCA+TGG + chr4.3:88913320-88913339 MS.gene053285:CDS 45.0%
!! GCTGTTTTGGGTACTCTTGC+TGG + chr4.3:88913457-88913476 MS.gene053285:CDS 50.0%
GGCGGGGTTGAAGAAATGGT+TGG + chr4.3:88913258-88913277 MS.gene053285:CDS 55.0%
TAGCGGCGGGGTTGAAGAAA+TGG + chr4.3:88913254-88913273 MS.gene053285:CDS 55.0%
CACGCCGTTGACGGTTGTAG+CGG + chr4.3:88913237-88913256 MS.gene053285:CDS 60.0%
GACGGTGAACACGCCGTTGA+CGG + chr4.3:88913228-88913247 MS.gene053285:CDS 60.0%
CCCGCCGCTACAACCGTCAA+CGG - chr4.3:88913244-88913263 None:intergenic 65.0%
CCGTTGACGGTTGTAGCGGC+GGG + chr4.3:88913241-88913260 MS.gene053285:CDS 65.0%
CGTTGACGGTTGTAGCGGCG+GGG + chr4.3:88913242-88913261 MS.gene053285:CDS 65.0%
GCCGTTGACGGTTGTAGCGG+CGG + chr4.3:88913240-88913259 MS.gene053285:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 88913217 88913501 88913217 ID=MS.gene053285
chr4.3 mRNA 88913217 88913501 88913217 ID=MS.gene053285.t1;Parent=MS.gene053285
chr4.3 exon 88913217 88913501 88913217 ID=MS.gene053285.t1.exon1;Parent=MS.gene053285.t1
chr4.3 CDS 88913217 88913501 88913217 ID=cds.MS.gene053285.t1;Parent=MS.gene053285.t1
Gene Sequence

>MS.gene053285

ACTAGGACGGGGACGGTGAACACGCCGTTGACGGTTGTAGCGGCGGGGTTGAAGAAATGGTTGGATATTTACAGTGGTGTGTTGTTGGTTAGGGTTTTGTTGAGTTGGTTTCCCAACATTCCATGGGAAAGACAACCATTGTCTGCAATTAGGGATCTTTGTGATCCTTATCTAAGTTTGTTTAGGAATGTTGTTCCACCTGTTTTTGATACTCTTGATATTAGTCCTCTTCTTGCTTTTGCTGTTTTGGGTACTCTTGCTGGTCTCCTCACTGTTCCTGTTTAG

Protein sequence

>MS.gene053285.t1

TRTGTVNTPLTVVAAGLKKWLDIYSGVLLVRVLLSWFPNIPWERQPLSAIRDLCDPYLSLFRNVVPPVFDTLDISPLLAFAVLGTLAGLLTVPV