Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34826.t1 | PNX85476.1 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 1.40E-89 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34826.t1 | Q9M2N5 | 36.7 | 215 | 125 | 4 | 1 | 214 | 491 | 695 | 1.3e-35 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34826.t1 | A0A2K3M3W2 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 9.9e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene34826 | 0.813371 | 2.65E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene34826 | 0.810265 | 1.26E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene34826 | 0.800215 | 1.60E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene34826 | 0.802803 | 4.72E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene34826 | 0.802684 | 4.99E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene34826 | 0.804436 | 2.16E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene34826 | 0.807384 | 5.19E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene34826 | 0.801222 | 9.97E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene34826 | 0.812188 | 4.81E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene34826 | 0.822237 | 2.64E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34826.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 491 | 674 | 6.26e-41 | 148 |
| MS.gene34826.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 469 | 652 | 6.55e-41 | 148 |
| MS.gene34826.t1 | AT1G18560 | 27.861 | 201 | 116 | 7 | 2 | 191 | 467 | 649 | 3.95e-13 | 68.6 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 8.3:+40079789 | None:intergenic |
| AACGGAGTTGGATCATTATC+TGG | 0.259746 | 8.3:-40080349 | MS.gene34826:CDS |
| TGCAGAAATTTGATAAATAT+TGG | 0.280085 | 8.3:-40080653 | MS.gene34826:CDS |
| CCACATCAGAATATCAAAAT+TGG | 0.289304 | 8.3:+40080302 | None:intergenic |
| TTTGGATCCAAGATACAAAA+TGG | 0.298966 | 8.3:-40080595 | MS.gene34826:CDS |
| GATGGGAGTTGCTACTGTTT+TGG | 0.320873 | 8.3:-40080613 | MS.gene34826:CDS |
| TCAGAAGCTACTGAAGTAAT+AGG | 0.323940 | 8.3:+40080216 | None:intergenic |
| ATATCAAAATTGGCACTTCT+AGG | 0.340733 | 8.3:+40080312 | None:intergenic |
| TTTGCAATTTCTTGCAATGT+TGG | 0.382104 | 8.3:+40080255 | None:intergenic |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 8.3:+40079918 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 8.3:+40079790 | None:intergenic |
| TAGCAACTCCCATCATATCC+TGG | 0.454437 | 8.3:+40080622 | None:intergenic |
| GCTAATTCTGTGGCTAATGA+TGG | 0.459373 | 8.3:-40080432 | MS.gene34826:CDS |
| ACTCTGTTGAGCAAGTTAAG+AGG | 0.474739 | 8.3:-40080533 | MS.gene34826:CDS |
| GAGTGTCACCCAGGATATGA+TGG | 0.476745 | 8.3:-40080631 | MS.gene34826:CDS |
| CTCATCGTAGTCGACTTCAT+TGG | 0.485204 | 8.3:-40080158 | MS.gene34826:CDS |
| ATATGGAGCAAGGAGAATAA+TGG | 0.491041 | 8.3:-40080099 | MS.gene34826:intron |
| TAGAACATCATTATATAAAA+CGG | 0.495038 | 8.3:-40080367 | MS.gene34826:CDS |
| ATCATTATATAAAACGGAGT+TGG | 0.499119 | 8.3:-40080361 | MS.gene34826:CDS |
| TAAAGAAATGGCTAAGAAGA+TGG | 0.502305 | 8.3:-40080676 | None:intergenic |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.508597 | 8.3:-40080122 | MS.gene34826:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 8.3:-40080145 | MS.gene34826:CDS |
| GAATCAGCATTCAGTACAAG+TGG | 0.537506 | 8.3:-40080195 | MS.gene34826:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 8.3:-40080148 | MS.gene34826:CDS |
| AGCAACTCCCATCATATCCT+GGG | 0.551068 | 8.3:+40080623 | None:intergenic |
| TGTGTGCTAGAAGTTGGATA+TGG | 0.560174 | 8.3:-40080116 | MS.gene34826:CDS |
| TAAATATTGGAGTGTCACCC+AGG | 0.565226 | 8.3:-40080640 | MS.gene34826:CDS |
| ACGGAGTTGGATCATTATCT+GGG | 0.571432 | 8.3:-40080348 | MS.gene34826:CDS |
| CTGATGTGGTGGAAGTTGAA+TGG | 0.578791 | 8.3:-40080288 | MS.gene34826:CDS |
| ATTATGATGCATATGTCAAG+AGG | 0.590157 | 8.3:-40080401 | MS.gene34826:CDS |
| AAGTTCAATCAAGACACTTG+TGG | 0.595420 | 8.3:-40080468 | MS.gene34826:CDS |
| AGACACTTGTGGTGAATCGT+CGG | 0.609844 | 8.3:-40080457 | MS.gene34826:CDS |
| AGTGTCACCCAGGATATGAT+GGG | 0.628166 | 8.3:-40080630 | MS.gene34826:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 8.3:+40080131 | None:intergenic |
| TAGAAGTTGGATATGGAGCA+AGG | 0.637735 | 8.3:-40080109 | MS.gene34826:CDS |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 8.3:-40079821 | MS.gene34826:intron |
| ATCGTCGGAAGCTAATTCTG+TGG | 0.696219 | 8.3:-40080442 | MS.gene34826:CDS |
| CAATGAAGTCGACTACGATG+AGG | 0.702001 | 8.3:+40080159 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | - | chr8.3:40080626-40080645 | MS.gene34826:CDS | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | + | chr8.3:40080598-40080617 | None:intergenic | 15.0% |
| !! | TAGAACATCATTATATAAAA+CGG | - | chr8.3:40080098-40080117 | MS.gene34826:intron | 15.0% |
| !!! | TACTATTTTGAAAAATTGTA+TGG | - | chr8.3:40079904-40079923 | MS.gene34826:intron | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | + | chr8.3:40080594-40080613 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | - | chr8.3:40080406-40080425 | MS.gene34826:CDS | 20.0% |
| !! | TGCAGAAATTTGATAAATAT+TGG | - | chr8.3:40079812-40079831 | MS.gene34826:CDS | 20.0% |
| ! | ATCATTATATAAAACGGAGT+TGG | - | chr8.3:40080104-40080123 | MS.gene34826:CDS | 25.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | + | chr8.3:40080577-40080596 | None:intergenic | 25.0% |
| AAGATCTTACATTCATCATC+AGG | + | chr8.3:40080550-40080569 | None:intergenic | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | - | chr8.3:40080469-40080488 | MS.gene34826:CDS | 30.0% | |
| ATATCAAAATTGGCACTTCT+AGG | + | chr8.3:40080156-40080175 | None:intergenic | 30.0% | |
| ATTATGATGCATATGTCAAG+AGG | - | chr8.3:40080064-40080083 | MS.gene34826:intron | 30.0% | |
| CCACATCAGAATATCAAAAT+TGG | + | chr8.3:40080166-40080185 | None:intergenic | 30.0% | |
| TTTGCAATTTCTTGCAATGT+TGG | + | chr8.3:40080213-40080232 | None:intergenic | 30.0% | |
| TTTGGATCCAAGATACAAAA+TGG | - | chr8.3:40079870-40079889 | MS.gene34826:intron | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | - | chr8.3:40080644-40080663 | MS.gene34826:CDS | 30.0% |
| !! | ATTTTGATATTCTGATGTGG+TGG | - | chr8.3:40080166-40080185 | MS.gene34826:CDS | 30.0% |
| !! | CCAATTTTGATATTCTGATG+TGG | - | chr8.3:40080163-40080182 | MS.gene34826:CDS | 30.0% |
| AAGTTCAATCAAGACACTTG+TGG | - | chr8.3:40079997-40080016 | MS.gene34826:intron | 35.0% | |
| ATATGGAGCAAGGAGAATAA+TGG | - | chr8.3:40080366-40080385 | MS.gene34826:CDS | 35.0% | |
| TCAGAAGCTACTGAAGTAAT+AGG | + | chr8.3:40080252-40080271 | None:intergenic | 35.0% | |
| ! | AGCAACTCCATTTTGTATCT+TGG | + | chr8.3:40079880-40079899 | None:intergenic | 35.0% |
| AACGGAGTTGGATCATTATC+TGG | - | chr8.3:40080116-40080135 | MS.gene34826:CDS | 40.0% | |
| ACGGAGTTGGATCATTATCT+GGG | - | chr8.3:40080117-40080136 | MS.gene34826:CDS | 40.0% | |
| ACTCTGTTGAGCAAGTTAAG+AGG | - | chr8.3:40079932-40079951 | MS.gene34826:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | - | chr8.3:40080343-40080362 | MS.gene34826:CDS | 40.0% | |
| GAATCAGCATTCAGTACAAG+TGG | - | chr8.3:40080270-40080289 | MS.gene34826:CDS | 40.0% | |
| GCTAATTCTGTGGCTAATGA+TGG | - | chr8.3:40080033-40080052 | MS.gene34826:intron | 40.0% | |
| TAGAAGTTGGATATGGAGCA+AGG | - | chr8.3:40080356-40080375 | MS.gene34826:CDS | 40.0% | |
| TGTGTGCTAGAAGTTGGATA+TGG | - | chr8.3:40080349-40080368 | MS.gene34826:CDS | 40.0% | |
| ! | TAAATATTGGAGTGTCACCC+AGG | - | chr8.3:40079825-40079844 | MS.gene34826:intron | 40.0% |
| ACTTCATTGGACAACCTTGG+AGG | - | chr8.3:40080320-40080339 | MS.gene34826:CDS | 45.0% | |
| AGACACTTGTGGTGAATCGT+CGG | - | chr8.3:40080008-40080027 | MS.gene34826:intron | 45.0% | |
| AGCAACTCCCATCATATCCT+GGG | + | chr8.3:40079845-40079864 | None:intergenic | 45.0% | |
| AGTGTCACCCAGGATATGAT+GGG | - | chr8.3:40079835-40079854 | MS.gene34826:intron | 45.0% | |
| ATCGTCGGAAGCTAATTCTG+TGG | - | chr8.3:40080023-40080042 | MS.gene34826:intron | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | + | chr8.3:40080309-40080328 | None:intergenic | 45.0% | |
| TAGCAACTCCCATCATATCC+TGG | + | chr8.3:40079846-40079865 | None:intergenic | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | - | chr8.3:40080317-40080336 | MS.gene34826:CDS | 45.0% | |
| ! | CTCATCGTAGTCGACTTCAT+TGG | - | chr8.3:40080307-40080326 | MS.gene34826:CDS | 45.0% |
| ! | CTGATGTGGTGGAAGTTGAA+TGG | - | chr8.3:40080177-40080196 | MS.gene34826:CDS | 45.0% |
| ! | GATGGGAGTTGCTACTGTTT+TGG | - | chr8.3:40079852-40079871 | MS.gene34826:intron | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | + | chr8.3:40080337-40080356 | None:intergenic | 50.0% | |
| GAGTGTCACCCAGGATATGA+TGG | - | chr8.3:40079834-40079853 | MS.gene34826:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 40079796 | 40080691 | 40079796 | ID=MS.gene34826 |
| chr8.3 | mRNA | 40079796 | 40080691 | 40079796 | ID=MS.gene34826.t1;Parent=MS.gene34826 |
| chr8.3 | exon | 40080100 | 40080691 | 40080100 | ID=MS.gene34826.t1.exon1;Parent=MS.gene34826.t1 |
| chr8.3 | CDS | 40080100 | 40080691 | 40080100 | ID=cds.MS.gene34826.t1;Parent=MS.gene34826.t1 |
| chr8.3 | exon | 40079928 | 40079996 | 40079928 | ID=MS.gene34826.t1.exon2;Parent=MS.gene34826.t1 |
| chr8.3 | CDS | 40079928 | 40079996 | 40079928 | ID=cds.MS.gene34826.t1;Parent=MS.gene34826.t1 |
| chr8.3 | exon | 40079796 | 40079839 | 40079796 | ID=MS.gene34826.t1.exon3;Parent=MS.gene34826.t1 |
| chr8.3 | CDS | 40079796 | 40079839 | 40079796 | ID=cds.MS.gene34826.t1;Parent=MS.gene34826.t1 |
| Gene Sequence |
| Protein sequence |
