Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35051.t1 | RHN62754.1 | 93.3 | 225 | 14 | 1 | 1 | 225 | 1 | 224 | 6.30E-92 | 347.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35051.t1 | Q9M2C0 | 57.7 | 201 | 75 | 2 | 26 | 225 | 7 | 198 | 2.5e-57 | 223.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35051.t1 | A0A396IER6 | 93.3 | 225 | 14 | 1 | 1 | 225 | 1 | 224 | 4.6e-92 | 347.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051325 | MS.gene35051 | 0.872635 | 2.73E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene48173 | MS.gene35051 | PPI |
| MS.gene35051 | MS.gene60673 | PPI |
| MS.gene67394 | MS.gene35051 | PPI |
| MS.gene35051 | MS.gene59206 | PPI |
| MS.gene000219 | MS.gene35051 | PPI |
| MS.gene006180 | MS.gene35051 | PPI |
| MS.gene35051 | MS.gene066222 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35051.t1 | MTR_4g094332 | 95.556 | 225 | 9 | 1 | 1 | 225 | 1 | 224 | 8.37e-137 | 383 |
| MS.gene35051.t1 | MTR_4g094328 | 93.778 | 225 | 13 | 1 | 1 | 225 | 1 | 224 | 1.38e-132 | 372 |
| MS.gene35051.t1 | MTR_4g094330 | 93.778 | 225 | 13 | 1 | 1 | 225 | 1 | 224 | 1.38e-132 | 372 |
| MS.gene35051.t1 | MTR_1g099010 | 65.347 | 202 | 65 | 1 | 24 | 225 | 21 | 217 | 3.56e-90 | 265 |
| MS.gene35051.t1 | MTR_6g034975 | 65.714 | 210 | 68 | 2 | 16 | 225 | 5 | 210 | 2.10e-87 | 257 |
| MS.gene35051.t1 | MTR_4g094325 | 61.033 | 213 | 78 | 2 | 13 | 225 | 32 | 239 | 4.56e-84 | 250 |
| MS.gene35051.t1 | MTR_2g008110 | 60.733 | 191 | 65 | 1 | 30 | 220 | 27 | 207 | 1.30e-72 | 220 |
| MS.gene35051.t1 | MTR_0119s0010 | 60.733 | 191 | 65 | 1 | 30 | 220 | 27 | 207 | 1.36e-72 | 219 |
| MS.gene35051.t1 | MTR_0119s0020 | 60.733 | 191 | 65 | 1 | 30 | 220 | 27 | 207 | 1.36e-72 | 219 |
| MS.gene35051.t1 | MTR_4g094335 | 57.561 | 205 | 79 | 2 | 18 | 220 | 27 | 225 | 2.51e-70 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35051.t1 | AT3G43660 | 61.692 | 201 | 67 | 2 | 26 | 225 | 7 | 198 | 7.57e-91 | 265 |
| MS.gene35051.t1 | AT1G21140 | 61.500 | 200 | 67 | 2 | 26 | 225 | 11 | 200 | 4.71e-81 | 241 |
| MS.gene35051.t1 | AT3G43630 | 61.000 | 200 | 68 | 2 | 24 | 222 | 4 | 194 | 3.44e-80 | 239 |
| MS.gene35051.t1 | AT1G76800 | 61.194 | 201 | 68 | 1 | 25 | 225 | 6 | 196 | 3.93e-79 | 236 |
| MS.gene35051.t1 | AT3G25190 | 63.033 | 211 | 73 | 2 | 18 | 225 | 9 | 217 | 4.10e-74 | 224 |
Find 52 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGCTATGGCCATTACTTT+TGG | 0.296297 | 4.4:-20483652 | MS.gene35051:CDS |
| ACCAAATTGATTGGCACTAC+TGG | 0.296615 | 4.4:-20483625 | MS.gene35051:CDS |
| TCTTGTGTTAGAGTTCTTAT+TGG | 0.297338 | 4.4:-20483682 | MS.gene35051:CDS |
| TGTGGTTAGCTTTGCTTTGT+TGG | 0.303507 | 4.4:-20483752 | MS.gene35051:CDS |
| TTCAACTTTGAGAGTACTAT+TGG | 0.313072 | 4.4:+20484200 | None:intergenic |
| TCCAGTAGTGCCAATCAATT+TGG | 0.330163 | 4.4:+20483624 | None:intergenic |
| TTTGGTTTCACCAAATTGAT+TGG | 0.341339 | 4.4:-20483634 | MS.gene35051:CDS |
| AGCTTTGCTTTGTTGGTGTT+CGG | 0.375879 | 4.4:-20483745 | MS.gene35051:CDS |
| CTGCATTTATAAAGAATCAT+AGG | 0.383563 | 4.4:-20483798 | MS.gene35051:CDS |
| ATCTCAACCTCAAAATTGTT+TGG | 0.384795 | 4.4:+20484234 | None:intergenic |
| ATCAAAGTAATGCTCCTTGC+TGG | 0.393708 | 4.4:-20484051 | MS.gene35051:CDS |
| AATGCTGCTTGAAATGGATT+TGG | 0.394757 | 4.4:+20483871 | None:intergenic |
| TCCTTCATGGCCTCACAACT+TGG | 0.407619 | 4.4:-20484279 | None:intergenic |
| GAAGCTAATGCTGCTTGAAA+TGG | 0.414506 | 4.4:+20483865 | None:intergenic |
| ATGCTCCTTGCTGGTTTCGC+TGG | 0.425505 | 4.4:-20484042 | MS.gene35051:CDS |
| TGGCTTAGAGCAGCAATGTT+AGG | 0.431906 | 4.4:-20484138 | MS.gene35051:CDS |
| TATTGGAGGTTGGATGGCTA+TGG | 0.439649 | 4.4:-20483665 | MS.gene35051:CDS |
| TGTGTTAGAGTTCTTATTGG+AGG | 0.446628 | 4.4:-20483679 | MS.gene35051:CDS |
| TTAGAGTTCTTATTGGAGGT+TGG | 0.455085 | 4.4:-20483675 | MS.gene35051:CDS |
| AAAGAATCATAGGATTAGAA+TGG | 0.457336 | 4.4:-20483788 | MS.gene35051:CDS |
| AACAGTGATTAGTCCATCAT+TGG | 0.469248 | 4.4:+20484113 | None:intergenic |
| GGTTTCGCTGGTTTAGTAGC+CGG | 0.470478 | 4.4:-20484030 | MS.gene35051:CDS |
| CAGCATTGATTACTTTCAAA+GGG | 0.487104 | 4.4:-20484166 | MS.gene35051:CDS |
| ACAGCATTGATTACTTTCAA+AGG | 0.499048 | 4.4:-20484167 | MS.gene35051:CDS |
| AAGAATCATAGGATTAGAAT+GGG | 0.503124 | 4.4:-20483787 | MS.gene35051:CDS |
| GGAGGAGTGGGTGCATTGGT+TGG | 0.503269 | 4.4:-20483724 | MS.gene35051:CDS |
| AGAGTACTATTGGCATGGAT+AGG | 0.503952 | 4.4:+20484210 | None:intergenic |
| AGTTCTTATTGGAGGTTGGA+TGG | 0.512108 | 4.4:-20483671 | MS.gene35051:CDS |
| GTTCGGAGGAGTGGGTGCAT+TGG | 0.514286 | 4.4:-20483728 | MS.gene35051:CDS |
| GCCTCATTGATGATCGGCGT+TGG | 0.515998 | 4.4:-20484090 | MS.gene35051:CDS |
| AATGGGAGTTGTTGCTGCTG+TGG | 0.525549 | 4.4:-20483770 | MS.gene35051:CDS |
| GAGTACTATTGGCATGGATA+GGG | 0.527712 | 4.4:+20484211 | None:intergenic |
| AACCTCAAAATTGTTTGGTG+AGG | 0.527938 | 4.4:+20484239 | None:intergenic |
| CTTTGAGAGTACTATTGGCA+TGG | 0.540482 | 4.4:+20484205 | None:intergenic |
| TTGTTGGTGTTCGGAGGAGT+GGG | 0.540620 | 4.4:-20483736 | MS.gene35051:CDS |
| TTTGGTGAAACCAAAAGTAA+TGG | 0.542621 | 4.4:+20483642 | None:intergenic |
| AGCAGCATTAGCTTCCGCAC+TGG | 0.542923 | 4.4:-20483857 | MS.gene35051:CDS |
| ATAAATGCAGCTGCTAGTAA+TGG | 0.554872 | 4.4:+20483811 | None:intergenic |
| TTTGTTGGTGTTCGGAGGAG+TGG | 0.555153 | 4.4:-20483737 | MS.gene35051:CDS |
| GGTGCTTGTAGCATGGCAAT+TGG | 0.557712 | 4.4:-20484009 | MS.gene35051:CDS |
| AATTGCCATGCTACAAGCAC+CGG | 0.567135 | 4.4:+20484011 | None:intergenic |
| AGTAGCCGGTGCTTGTAGCA+TGG | 0.572629 | 4.4:-20484016 | MS.gene35051:CDS |
| TTTGCTTTGTTGGTGTTCGG+AGG | 0.573531 | 4.4:-20483742 | MS.gene35051:CDS |
| GCAATGTTAGGAGCCAATGA+TGG | 0.586770 | 4.4:-20484126 | MS.gene35051:CDS |
| ACTGTTGCCTCATTGATGAT+CGG | 0.595345 | 4.4:-20484096 | MS.gene35051:CDS |
| CTAACACAAGACCTCATCAA+AGG | 0.604425 | 4.4:+20483694 | None:intergenic |
| TGATGATGAAAGTAGTGAAA+GGG | 0.611622 | 4.4:-20483902 | MS.gene35051:CDS |
| ATTACTTTCAAAGGGCACAA+TGG | 0.615521 | 4.4:-20484158 | MS.gene35051:CDS |
| ATGATGATGAAAGTAGTGAA+AGG | 0.636055 | 4.4:-20483903 | MS.gene35051:CDS |
| CTAAACCAGCGAAACCAGCA+AGG | 0.672858 | 4.4:+20484037 | None:intergenic |
| TCCAACGCCGATCATCAATG+AGG | 0.683388 | 4.4:+20484089 | None:intergenic |
| TCCAATAGAAAATGCCAGTG+CGG | 0.690209 | 4.4:+20483843 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAATCATAGGATTAGAA+TGG | - | chr4.4:20484103-20484122 | MS.gene35051:CDS | 25.0% |
| ! | AAGAATCATAGGATTAGAAT+GGG | - | chr4.4:20484104-20484123 | MS.gene35051:CDS | 25.0% |
| ! | CTGCATTTATAAAGAATCAT+AGG | - | chr4.4:20484093-20484112 | MS.gene35051:CDS | 25.0% |
| ATCTCAACCTCAAAATTGTT+TGG | + | chr4.4:20483660-20483679 | None:intergenic | 30.0% | |
| TCTTGTGTTAGAGTTCTTAT+TGG | - | chr4.4:20484209-20484228 | MS.gene35051:CDS | 30.0% | |
| TTTGGTGAAACCAAAAGTAA+TGG | + | chr4.4:20484252-20484271 | None:intergenic | 30.0% | |
| ! | ATGATGATGAAAGTAGTGAA+AGG | - | chr4.4:20483988-20484007 | MS.gene35051:CDS | 30.0% |
| ! | TGATGATGAAAGTAGTGAAA+GGG | - | chr4.4:20483989-20484008 | MS.gene35051:CDS | 30.0% |
| ! | TTTGGTTTCACCAAATTGAT+TGG | - | chr4.4:20484257-20484276 | MS.gene35051:CDS | 30.0% |
| !! | ACAGCATTGATTACTTTCAA+AGG | - | chr4.4:20483724-20483743 | MS.gene35051:CDS | 30.0% |
| !! | CAGCATTGATTACTTTCAAA+GGG | - | chr4.4:20483725-20483744 | MS.gene35051:CDS | 30.0% |
| !! | TTCAACTTTGAGAGTACTAT+TGG | + | chr4.4:20483694-20483713 | None:intergenic | 30.0% |
| ATAAATGCAGCTGCTAGTAA+TGG | + | chr4.4:20484083-20484102 | None:intergenic | 35.0% | |
| CAACTTGGAGCAAAAACTAA+TGG | - | chr4.4:20483627-20483646 | MS.gene35051:CDS | 35.0% | |
| TGTGTTAGAGTTCTTATTGG+AGG | - | chr4.4:20484212-20484231 | MS.gene35051:CDS | 35.0% | |
| TTAGAGTTCTTATTGGAGGT+TGG | - | chr4.4:20484216-20484235 | MS.gene35051:CDS | 35.0% | |
| TTGGAAAAACTCCTTTGATG+AGG | - | chr4.4:20484186-20484205 | MS.gene35051:CDS | 35.0% | |
| ! | AACAGTGATTAGTCCATCAT+TGG | + | chr4.4:20483781-20483800 | None:intergenic | 35.0% |
| ! | AACCTCAAAATTGTTTGGTG+AGG | + | chr4.4:20483655-20483674 | None:intergenic | 35.0% |
| ! | AACCTCACCAAACAATTTTG+AGG | - | chr4.4:20483650-20483669 | MS.gene35051:CDS | 35.0% |
| ! | AATGCTGCTTGAAATGGATT+TGG | + | chr4.4:20484023-20484042 | None:intergenic | 35.0% |
| ! | ATTACTTTCAAAGGGCACAA+TGG | - | chr4.4:20483733-20483752 | MS.gene35051:CDS | 35.0% |
| ACCAAATTGATTGGCACTAC+TGG | - | chr4.4:20484266-20484285 | MS.gene35051:CDS | 40.0% | |
| AGTTCTTATTGGAGGTTGGA+TGG | - | chr4.4:20484220-20484239 | MS.gene35051:CDS | 40.0% | |
| ATCAAAGTAATGCTCCTTGC+TGG | - | chr4.4:20483840-20483859 | MS.gene35051:CDS | 40.0% | |
| CTAACACAAGACCTCATCAA+AGG | + | chr4.4:20484200-20484219 | None:intergenic | 40.0% | |
| GAGTACTATTGGCATGGATA+GGG | + | chr4.4:20483683-20483702 | None:intergenic | 40.0% | |
| TCCAATAGAAAATGCCAGTG+CGG | + | chr4.4:20484051-20484070 | None:intergenic | 40.0% | |
| TCCAGTAGTGCCAATCAATT+TGG | + | chr4.4:20484270-20484289 | None:intergenic | 40.0% | |
| ! | ACTGTTGCCTCATTGATGAT+CGG | - | chr4.4:20483795-20483814 | MS.gene35051:CDS | 40.0% |
| ! | AGAGTACTATTGGCATGGAT+AGG | + | chr4.4:20483684-20483703 | None:intergenic | 40.0% |
| ! | AGCTTTGCTTTGTTGGTGTT+CGG | - | chr4.4:20484146-20484165 | MS.gene35051:CDS | 40.0% |
| ! | AGTTTTTGCTCCAAGTTGTG+AGG | + | chr4.4:20483625-20483644 | None:intergenic | 40.0% |
| ! | ATGGCTATGGCCATTACTTT+TGG | - | chr4.4:20484239-20484258 | MS.gene35051:CDS | 40.0% |
| ! | GAAGCTAATGCTGCTTGAAA+TGG | + | chr4.4:20484029-20484048 | None:intergenic | 40.0% |
| !! | CTTTGAGAGTACTATTGGCA+TGG | + | chr4.4:20483689-20483708 | None:intergenic | 40.0% |
| !! | TGTGGTTAGCTTTGCTTTGT+TGG | - | chr4.4:20484139-20484158 | MS.gene35051:CDS | 40.0% |
| AATTGCCATGCTACAAGCAC+CGG | + | chr4.4:20483883-20483902 | None:intergenic | 45.0% | |
| GCAATGTTAGGAGCCAATGA+TGG | - | chr4.4:20483765-20483784 | MS.gene35051:CDS | 45.0% | |
| TATTGGAGGTTGGATGGCTA+TGG | - | chr4.4:20484226-20484245 | MS.gene35051:CDS | 45.0% | |
| TGGCTTAGAGCAGCAATGTT+AGG | - | chr4.4:20483753-20483772 | MS.gene35051:CDS | 45.0% | |
| ! | TTTGCTTTGTTGGTGTTCGG+AGG | - | chr4.4:20484149-20484168 | MS.gene35051:CDS | 45.0% |
| !! | TCCGCACTGGCATTTTCTAT+TGG | - | chr4.4:20484047-20484066 | MS.gene35051:CDS | 45.0% |
| AATGGGAGTTGTTGCTGCTG+TGG | - | chr4.4:20484121-20484140 | MS.gene35051:CDS | 50.0% | |
| CTAAACCAGCGAAACCAGCA+AGG | + | chr4.4:20483857-20483876 | None:intergenic | 50.0% | |
| GGTGCTTGTAGCATGGCAAT+TGG | - | chr4.4:20483882-20483901 | MS.gene35051:CDS | 50.0% | |
| TCCAACGCCGATCATCAATG+AGG | + | chr4.4:20483805-20483824 | None:intergenic | 50.0% | |
| ! | TTGTTGGTGTTCGGAGGAGT+GGG | - | chr4.4:20484155-20484174 | MS.gene35051:CDS | 50.0% |
| ! | TTTGTTGGTGTTCGGAGGAG+TGG | - | chr4.4:20484154-20484173 | MS.gene35051:CDS | 50.0% |
| !! | GGTTTCGCTGGTTTAGTAGC+CGG | - | chr4.4:20483861-20483880 | MS.gene35051:CDS | 50.0% |
| AGTAGCCGGTGCTTGTAGCA+TGG | - | chr4.4:20483875-20483894 | MS.gene35051:CDS | 55.0% | |
| ! | AGCAGCATTAGCTTCCGCAC+TGG | - | chr4.4:20484034-20484053 | MS.gene35051:CDS | 55.0% |
| ! | GCCTCATTGATGATCGGCGT+TGG | - | chr4.4:20483801-20483820 | MS.gene35051:CDS | 55.0% |
| !! | ATGCTCCTTGCTGGTTTCGC+TGG | - | chr4.4:20483849-20483868 | MS.gene35051:CDS | 55.0% |
| GTTCGGAGGAGTGGGTGCAT+TGG | - | chr4.4:20484163-20484182 | MS.gene35051:CDS | 60.0% | |
| ! | GGAGGAGTGGGTGCATTGGT+TGG | - | chr4.4:20484167-20484186 | MS.gene35051:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20483618 | 20484295 | 20483618 | ID=MS.gene35051 |
| chr4.4 | mRNA | 20483618 | 20484295 | 20483618 | ID=MS.gene35051.t1;Parent=MS.gene35051 |
| chr4.4 | exon | 20483618 | 20484295 | 20483618 | ID=MS.gene35051.t1.exon1;Parent=MS.gene35051.t1 |
| chr4.4 | CDS | 20483618 | 20484295 | 20483618 | ID=cds.MS.gene35051.t1;Parent=MS.gene35051.t1 |
| Gene Sequence |
| Protein sequence |
