AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35289

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35289.t1 MTR_2g084230 94.574 258 8 1 1 252 1 258 1.16e-180 501
MS.gene35289.t1 MTR_2g084230 94.574 258 8 1 1 252 1 258 9.93e-180 496
MS.gene35289.t1 MTR_2g016220 57.895 152 50 4 35 183 37 177 1.06e-45 157
MS.gene35289.t1 MTR_8g086410 64.493 138 43 2 32 167 46 179 1.07e-45 158
MS.gene35289.t1 MTR_8g086410 64.493 138 43 2 32 167 48 181 1.18e-45 158
MS.gene35289.t1 MTR_2g016220 57.895 152 50 4 35 183 37 177 1.24e-45 157
MS.gene35289.t1 MTR_2g016220 56.410 156 50 5 35 183 37 181 1.67e-43 151
MS.gene35289.t1 MTR_8g086410 61.379 145 43 3 32 167 48 188 5.22e-43 151
MS.gene35289.t1 MTR_7g069660 66.667 111 36 1 52 162 27 136 1.67e-42 148
MS.gene35289.t1 MTR_2g023580 59.140 93 38 0 75 167 33 125 6.54e-33 120
MS.gene35289.t1 MTR_0223s0040 69.737 76 23 0 84 159 5 80 5.41e-32 119
MS.gene35289.t1 MTR_1g112370 60.000 90 33 2 83 169 8 97 6.42e-31 117
MS.gene35289.t1 MTR_3g450310 55.696 79 29 1 75 153 3 75 1.55e-23 95.1
MS.gene35289.t1 MTR_1g021520 64.789 71 22 2 89 158 120 188 8.24e-22 92.4
MS.gene35289.t1 MTR_6g071625 68.966 58 18 0 96 153 201 258 9.82e-21 90.5
MS.gene35289.t1 MTR_5g029470 68.966 58 18 0 96 153 218 275 1.99e-20 89.7
MS.gene35289.t1 MTR_3g064840 65.574 61 21 0 96 156 273 333 2.73e-20 89.7
MS.gene35289.t1 MTR_8g066310 63.934 61 22 0 96 156 172 232 4.75e-20 88.2
MS.gene35289.t1 MTR_7g115530 60.294 68 26 1 98 164 219 286 7.75e-20 87.0
MS.gene35289.t1 MTR_7g098250 67.857 56 18 0 98 153 263 318 1.15e-19 88.2
MS.gene35289.t1 MTR_7g115530 60.294 68 26 1 98 164 219 286 1.86e-19 86.3
MS.gene35289.t1 MTR_1g080330 61.194 67 25 1 87 153 166 231 1.88e-19 85.5
MS.gene35289.t1 MTR_7g115530 49.425 87 43 1 98 183 219 305 1.90e-19 87.4
MS.gene35289.t1 MTR_1g080330 61.194 67 25 1 87 153 166 231 2.20e-19 86.7
MS.gene35289.t1 MTR_7g093030 50.000 96 40 3 93 187 208 296 2.79e-19 86.3
MS.gene35289.t1 MTR_7g093030 51.064 94 38 3 93 185 208 294 2.87e-19 85.1
MS.gene35289.t1 MTR_7g115530 66.071 56 19 0 98 153 219 274 3.84e-19 85.9
MS.gene35289.t1 MTR_7g115530 67.308 52 17 0 98 149 219 270 1.21e-17 80.9
MS.gene35289.t1 MTR_1g053835 50.000 96 39 3 70 164 239 326 1.33e-17 82.0
MS.gene35289.t1 MTR_7g115530 64.286 56 20 0 98 153 219 274 1.34e-17 80.5
MS.gene35289.t1 MTR_5g027440 60.345 58 23 0 93 150 21 78 2.72e-17 79.7
MS.gene35289.t1 MTR_6g032990 62.264 53 20 0 98 150 43 95 3.53e-17 80.5
MS.gene35289.t1 MTR_1g053830 49.333 75 37 1 99 172 20 94 4.42e-17 74.7
MS.gene35289.t1 MTR_2g086450 58.929 56 23 0 98 153 46 101 4.44e-17 80.1
MS.gene35289.t1 MTR_2g027800 42.857 105 50 2 98 202 40 134 1.33e-16 78.2
MS.gene35289.t1 MTR_7g088070 65.000 60 20 1 99 157 52 111 1.44e-16 77.8
MS.gene35289.t1 MTR_7g088070 65.000 60 20 1 99 157 52 111 1.51e-16 77.4
MS.gene35289.t1 MTR_1g090670 60.714 56 22 0 98 153 45 100 2.40e-16 77.0
MS.gene35289.t1 MTR_2g027800 62.264 53 20 0 98 150 40 92 4.90e-16 76.3
MS.gene35289.t1 MTR_2g027860 65.455 55 19 0 99 153 51 105 1.34e-15 75.5
MS.gene35289.t1 MTR_5g041350 52.830 53 25 0 98 150 16 68 2.20e-15 75.1
MS.gene35289.t1 MTR_6g444980 55.357 56 25 0 98 153 36 91 1.40e-14 72.8
MS.gene35289.t1 MTR_6g444980 55.357 56 25 0 98 153 36 91 1.60e-14 72.4
MS.gene35289.t1 MTR_4g081710 52.830 53 25 0 98 150 18 70 7.99e-14 70.5
MS.gene35289.t1 MTR_1g053800 58.491 53 22 0 99 151 179 231 1.12e-13 70.1
MS.gene35289.t1 MTR_7g068600 61.017 59 20 1 99 154 15 73 2.20e-13 68.2
MS.gene35289.t1 MTR_7g093010 58.491 53 22 0 99 151 215 267 5.46e-12 65.1
MS.gene35289.t1 MTR_4g021790 43.243 74 38 2 80 150 148 220 5.81e-12 64.7
MS.gene35289.t1 MTR_4g113140 39.130 92 48 1 67 150 197 288 1.25e-11 64.3
MS.gene35289.t1 MTR_4g086835 45.455 66 36 0 85 150 262 327 1.40e-11 64.3
MS.gene35289.t1 MTR_2g450070 52.727 55 25 1 96 150 212 265 2.28e-11 63.9
MS.gene35289.t1 MTR_2g450070 52.727 55 25 1 96 150 212 265 2.38e-11 63.9
MS.gene35289.t1 MTR_4g064730 53.125 64 29 1 87 150 121 183 2.43e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35289.t1 AT2G38300 73.256 86 23 0 77 162 34 119 2.02e-38 137
MS.gene35289.t1 AT2G42660 67.021 94 20 2 89 177 42 129 1.57e-35 127
MS.gene35289.t1 AT2G40260 66.667 96 23 1 70 156 46 141 6.95e-34 127
MS.gene35289.t1 AT4G04605 65.882 85 27 1 89 171 6 90 5.55e-32 117
MS.gene35289.t1 AT2G02060 67.059 85 21 2 66 150 5 82 6.17e-29 110
MS.gene35289.t1 AT2G02060 67.059 85 21 2 66 150 5 82 6.17e-29 110
MS.gene35289.t1 AT1G14600 67.606 71 23 0 89 159 15 85 5.48e-28 107
MS.gene35289.t1 AT4G04555 61.644 73 28 0 85 157 2 74 1.27e-25 97.4
MS.gene35289.t1 AT4G04580 59.155 71 29 0 89 159 6 76 3.13e-24 95.9
MS.gene35289.t1 AT5G42630 65.079 63 22 0 96 158 104 166 2.33e-22 92.4
MS.gene35289.t1 AT5G42630 65.079 63 22 0 96 158 104 166 4.58e-22 92.8
MS.gene35289.t1 AT1G32240 61.194 67 26 0 96 162 212 278 1.26e-20 90.1
MS.gene35289.t1 AT4G17695 62.319 69 24 1 88 156 157 223 1.52e-20 89.4
MS.gene35289.t1 AT1G32240 68.966 58 18 0 96 153 212 269 1.81e-20 89.7
MS.gene35289.t1 AT5G16560 68.966 58 18 0 96 153 218 275 2.38e-20 89.7
MS.gene35289.t1 AT5G29000 36.250 160 89 5 5 153 130 287 2.87e-20 88.2
MS.gene35289.t1 AT5G29000 36.250 160 89 5 5 153 130 287 9.77e-20 88.2
MS.gene35289.t1 AT5G29000 36.250 160 89 5 5 153 87 244 1.11e-19 87.4
MS.gene35289.t1 AT5G29000 36.250 160 89 5 5 153 87 244 1.11e-19 87.4
MS.gene35289.t1 AT4G28610 48.276 87 43 1 99 183 227 313 3.26e-19 86.7
MS.gene35289.t1 AT5G45580 47.191 89 42 1 93 181 3 86 7.20e-19 83.6
MS.gene35289.t1 AT5G45580 47.191 89 42 1 93 181 19 102 1.02e-18 83.6
MS.gene35289.t1 AT3G04450 52.778 72 29 1 99 165 240 311 2.54e-18 83.2
MS.gene35289.t1 AT3G04450 61.818 55 21 0 99 153 200 254 1.07e-17 82.0
MS.gene35289.t1 AT3G04450 61.818 55 21 0 99 153 200 254 1.07e-17 82.0
MS.gene35289.t1 AT3G04450 61.818 55 21 0 99 153 240 294 1.14e-17 82.4
MS.gene35289.t1 AT3G13040 67.273 55 18 0 99 153 243 297 5.43e-17 80.1
MS.gene35289.t1 AT3G13040 67.273 55 18 0 99 153 243 297 5.43e-17 80.1
MS.gene35289.t1 AT3G13040 67.273 55 18 0 99 153 243 297 5.43e-17 80.1
MS.gene35289.t1 AT5G18240 43.269 104 56 2 65 167 13 114 7.57e-17 79.7
MS.gene35289.t1 AT5G18240 43.269 104 56 2 65 167 13 114 7.57e-17 79.7
MS.gene35289.t1 AT5G18240 43.269 104 56 2 65 167 13 114 8.17e-17 79.7
MS.gene35289.t1 AT5G18240 43.269 104 56 2 65 167 13 114 8.99e-17 79.3
MS.gene35289.t1 AT5G18240 43.269 104 56 2 65 167 13 114 8.99e-17 79.3
MS.gene35289.t1 AT2G06020 47.561 82 40 1 80 158 66 147 1.70e-16 77.8
MS.gene35289.t1 AT3G04030 55.932 59 25 1 98 155 46 104 2.45e-16 78.2
MS.gene35289.t1 AT3G04030 55.932 59 25 1 98 155 46 104 2.77e-16 78.2
MS.gene35289.t1 AT2G01060 55.844 77 21 2 99 162 17 93 6.90e-16 75.9
MS.gene35289.t1 AT3G12730 35.833 120 64 2 98 213 24 134 9.73e-16 74.7
MS.gene35289.t1 AT2G20400 63.636 55 20 0 99 153 233 287 1.58e-15 75.9
MS.gene35289.t1 AT2G20400 63.636 55 20 0 99 153 233 287 1.60e-15 75.9
MS.gene35289.t1 AT5G06800 56.452 62 26 1 99 159 194 255 1.94e-15 74.7
MS.gene35289.t1 AT4G13640 56.604 53 23 0 98 150 38 90 4.06e-15 73.9
MS.gene35289.t1 AT3G24120 56.604 53 23 0 98 150 42 94 4.06e-15 73.9
MS.gene35289.t1 AT3G24120 56.604 53 23 0 98 150 42 94 4.14e-15 73.9
MS.gene35289.t1 AT4G13640 56.604 53 23 0 98 150 38 90 4.18e-15 73.9
MS.gene35289.t1 AT5G06800 56.452 62 26 1 99 159 194 255 7.64e-15 73.6
MS.gene35289.t1 AT5G06800 56.452 62 26 1 99 159 194 255 7.81e-15 73.6
MS.gene35289.t1 AT1G79430 55.357 56 25 0 98 153 35 90 1.04e-14 73.2
MS.gene35289.t1 AT1G69580 34.711 121 73 3 98 213 35 154 1.05e-14 73.2
MS.gene35289.t1 AT1G69580 40.426 94 51 2 98 186 35 128 1.41e-14 72.8
MS.gene35289.t1 AT3G04030 54.237 59 25 2 98 155 46 103 7.29e-14 70.9
MS.gene35289.t1 AT5G59570 57.143 56 23 1 95 150 139 193 1.06e-11 63.9
MS.gene35289.t1 AT5G59570 57.143 56 23 1 95 150 139 193 1.06e-11 63.9
MS.gene35289.t1 AT3G62670 44.872 78 38 2 78 150 191 268 1.12e-11 64.7
MS.gene35289.t1 AT2G03500 50.746 67 31 1 84 150 222 286 1.29e-11 64.3
MS.gene35289.t1 AT3G16857 43.158 95 49 3 60 150 126 219 2.83e-11 63.5
MS.gene35289.t1 AT4G31920 40.000 105 61 2 85 189 171 273 3.41e-11 63.2
MS.gene35289.t1 AT3G16857 43.158 95 49 3 60 150 196 289 3.43e-11 63.5
MS.gene35289.t1 AT3G16857 43.158 95 49 3 60 150 196 289 4.13e-11 63.2
MS.gene35289.t1 AT4G37180 46.970 66 35 0 85 150 198 263 4.48e-11 62.8
MS.gene35289.t1 AT3G46640 56.364 55 23 1 96 150 143 196 4.53e-11 62.4
MS.gene35289.t1 AT3G46640 56.364 55 23 1 96 150 143 196 4.53e-11 62.4
MS.gene35289.t1 AT4G37180 46.970 66 35 0 85 150 205 270 5.26e-11 62.4
MS.gene35289.t1 AT5G05090 52.000 75 33 3 77 150 61 133 5.75e-11 61.6
MS.gene35289.t1 AT3G46640 56.364 55 23 1 96 150 143 196 5.82e-11 62.0
MS.gene35289.t1 AT1G25550 53.846 52 24 0 99 150 211 262 7.91e-11 62.0
MS.gene35289.t1 AT3G10760 43.333 90 46 4 64 150 70 157 9.40e-11 61.6

Find 49 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATATTGCACCTATATT+AGG 0.306848 2.1:-17449490 MS.gene35289:CDS
GCAAGGAAGGCATCACATTA+TGG 0.348737 2.1:-17450154 MS.gene35289:CDS
GTCCTCTCACATTCATTAAC+TGG 0.352977 2.1:+17450821 None:intergenic
CATTAACTGGAGAACTAACT+TGG 0.357662 2.1:+17450834 None:intergenic
CGGGTAGTAATTGACTTACT+TGG 0.374141 2.1:+17449462 None:intergenic
TTCACATCTATTTGATGTAA+AGG 0.385780 2.1:-17449527 MS.gene35289:CDS
TCAAAGCTCATGACACTTCA+AGG 0.388453 2.1:-17450050 MS.gene35289:intron
ACATGTGAAGAGTCATTTGC+AGG 0.413814 2.1:-17450788 MS.gene35289:intron
AATAATAATTCTAGTGAAGA+AGG 0.443311 2.1:-17451555 MS.gene35289:CDS
GTGCATGCTGTTGAGAGGCT+TGG 0.454830 2.1:-17451447 MS.gene35289:CDS
CATCACATTATGGATATGTA+TGG 0.459114 2.1:-17450144 MS.gene35289:CDS
ACAAAAGATAAATGAAGATC+AGG 0.462114 2.1:+17451468 None:intergenic
AGTGAAAGTAGTAGCGGTAT+CGG 0.469081 2.1:-17451741 MS.gene35289:CDS
CTTCGAATCGGTAAAGAGGC+AGG 0.473218 2.1:+17451711 None:intergenic
AATTCTAGTGAAGAAGGAAA+AGG 0.475804 2.1:-17451549 MS.gene35289:CDS
TTTGTAGATTTCATGAGAGT+GGG 0.483163 2.1:-17449606 MS.gene35289:intron
CATGCTGTTGAGAGGCTTGG+AGG 0.487742 2.1:-17451444 MS.gene35289:CDS
CGAAAATGCCTAGGCTTCGT+TGG 0.491348 2.1:-17451494 MS.gene35289:CDS
ATTCTAGTGAAGAAGGAAAA+GGG 0.515576 2.1:-17451548 MS.gene35289:CDS
AACGAAGAAGCCGTGGACGA+TGG 0.526235 2.1:-17451666 MS.gene35289:CDS
AGGAAAAGGGCGTGGAAGTA+CGG 0.529396 2.1:-17451535 MS.gene35289:CDS
AAAATAGAGCAATGCAAGGA+AGG 0.540544 2.1:-17450167 MS.gene35289:CDS
TATGATGGTTGTTTCAGTAG+AGG 0.540634 2.1:+17450081 None:intergenic
TACTTGGCTAGACCTAATAT+AGG 0.549092 2.1:+17449478 None:intergenic
TCACTCTTGACATCTTTGCT+AGG 0.551418 2.1:+17451759 None:intergenic
GCTTTGATTTCATTATATGA+TGG 0.554601 2.1:+17450066 None:intergenic
TCAGGAGTCCAACGAAGCCT+AGG 0.559435 2.1:+17451486 None:intergenic
AATGAGATATCATCACAAGA+AGG 0.560760 2.1:-17451591 MS.gene35289:CDS
AAGGGCGTGGAAGTACGGTT+AGG 0.570454 2.1:-17451530 MS.gene35289:CDS
ATGAGATATCATCACAAGAA+GGG 0.586610 2.1:-17451590 MS.gene35289:CDS
ATGGATATGTATGGACACTT+TGG 0.587338 2.1:-17450135 MS.gene35289:CDS
AGTGAAGAAGGAAAAGGGCG+TGG 0.587852 2.1:-17451543 MS.gene35289:CDS
ATTAACTGGAGAACTAACTT+GGG 0.599704 2.1:+17450835 None:intergenic
ATGATCATCTCCATCGTCCA+CGG 0.603158 2.1:+17451656 None:intergenic
CTTCAGATCAAGTTCAGTGT+GGG 0.604465 2.1:-17449560 MS.gene35289:CDS
ATGTGAGGTCGAAAATGCCT+AGG 0.605149 2.1:-17451503 MS.gene35289:CDS
TTGTTGCTTATTTCACCACA+AGG 0.610333 2.1:+17451612 None:intergenic
ATGCTGTTGAGAGGCTTGGA+GGG 0.612873 2.1:-17451443 MS.gene35289:CDS
GTCAAGAGTGAAAGTAGTAG+CGG 0.626941 2.1:-17451747 MS.gene35289:CDS
AAGCTTGATGAGTCTGGCCA+AGG 0.633707 2.1:-17450325 MS.gene35289:intron
CGATTTAAACGAAGAAGCCG+TGG 0.635364 2.1:-17451673 MS.gene35289:CDS
TGTTTCAGTAGAGGAGACGA+TGG 0.646955 2.1:+17450090 None:intergenic
CTCCAGTTAATGAATGTGAG+AGG 0.648001 2.1:-17450823 MS.gene35289:CDS
GTTTCAGTAGAGGAGACGAT+GGG 0.648534 2.1:+17450091 None:intergenic
TGTTGCTTATTTCACCACAA+GGG 0.657001 2.1:+17451613 None:intergenic
GTGATAAGTGAAGGCCCTTG+TGG 0.681709 2.1:-17451627 MS.gene35289:CDS
TCTTCAGATCAAGTTCAGTG+TGG 0.702689 2.1:-17449561 MS.gene35289:CDS
CATGTGATAGTGATAAGTGA+AGG 0.715293 2.1:-17451636 MS.gene35289:CDS
GTACGGTTAGGCAATATGTG+AGG 0.731217 2.1:-17451518 MS.gene35289:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAATAAAATTCCA+TGG - chr2.1:17451284-17451303 MS.gene35289:intron 10.0%
!! TATATAAGAAAAATATGTAA+TGG + chr2.1:17451542-17451561 None:intergenic 10.0%
!! ACATAATTTAGTGTAATTAT+AGG + chr2.1:17450644-17450663 None:intergenic 15.0%
!!! AAATAGAAACATTTAAAACA+TGG - chr2.1:17451587-17451606 MS.gene35289:CDS 15.0%
!!! AATTTGATGTATTTATTTTG+AGG - chr2.1:17450357-17450376 MS.gene35289:intron 15.0%
!!! ATTTGATGTATTTATTTTGA+GGG - chr2.1:17450358-17450377 MS.gene35289:intron 15.0%
!!! CGTTTTAATTATGATTTTTT+TGG - chr2.1:17450883-17450902 MS.gene35289:intron 15.0%
!!! TTTAATTATGATTTTTTTGG+AGG - chr2.1:17450886-17450905 MS.gene35289:intron 15.0%
!! AATAATAATTCTAGTGAAGA+AGG - chr2.1:17449699-17449718 MS.gene35289:intron 20.0%
!! AATAATATTGCACCTATATT+AGG - chr2.1:17451764-17451783 MS.gene35289:CDS 20.0%
!! ACATTAAATGATTGAATGTT+GGG + chr2.1:17451404-17451423 None:intergenic 20.0%
!! AGATTAATTGTGTTTGTAAT+GGG - chr2.1:17450738-17450757 MS.gene35289:intron 20.0%
!! AGTAATAGTGCAAAATATTT+GGG + chr2.1:17451496-17451515 None:intergenic 20.0%
!! CACTTAATCTTATATAGATA+TGG - chr2.1:17450318-17450337 MS.gene35289:intron 20.0%
!! CATAAATTTATCATTACCTT+TGG - chr2.1:17449870-17449889 MS.gene35289:intron 20.0%
!! GGTAATATATATTCTTGTAT+AGG - chr2.1:17450487-17450506 MS.gene35289:intron 20.0%
!! TAACAATATTGGATATATGT+AGG - chr2.1:17450389-17450408 MS.gene35289:intron 20.0%
!! TAGATTAATTGTGTTTGTAA+TGG - chr2.1:17450737-17450756 MS.gene35289:intron 20.0%
!! TAGTAATAGTGCAAAATATT+TGG + chr2.1:17451497-17451516 None:intergenic 20.0%
!!! AATTTTACCACAAAACTTTA+GGG - chr2.1:17451457-17451476 MS.gene35289:CDS 20.0%
!!! TAATTTTACCACAAAACTTT+AGG - chr2.1:17451456-17451475 MS.gene35289:CDS 20.0%
!!! TGATTTTCTCACATTATTAA+CGG - chr2.1:17450663-17450682 MS.gene35289:intron 20.0%
!!! TGATTTTGCTAACAATATTA+AGG + chr2.1:17450967-17450986 None:intergenic 20.0%
! AAAAATGAGAAACAGTTGAT+AGG + chr2.1:17450263-17450282 None:intergenic 25.0%
! AATTCAGAGATCAATATTGT+CGG + chr2.1:17450112-17450131 None:intergenic 25.0%
! ACAAAAGATAAATGAAGATC+AGG + chr2.1:17449789-17449808 None:intergenic 25.0%
! ATCCATATTATAGTATGAGT+TGG + chr2.1:17450239-17450258 None:intergenic 25.0%
! ATTCAGAATCGTAATACATT+AGG + chr2.1:17450016-17450035 None:intergenic 25.0%
! GACATTAAATGATTGAATGT+TGG + chr2.1:17451405-17451424 None:intergenic 25.0%
! GATCTCTGAATTATATCTTT+AGG - chr2.1:17450120-17450139 MS.gene35289:CDS 25.0%
! TAATGTATTACGATTCTGAA+TGG - chr2.1:17450015-17450034 MS.gene35289:intron 25.0%
! TCCATATTATAGTATGAGTT+GGG + chr2.1:17450238-17450257 None:intergenic 25.0%
! TTATGAGTTCTGTATTTGTT+GGG - chr2.1:17449909-17449928 MS.gene35289:intron 25.0%
! TTCACATCTATTTGATGTAA+AGG - chr2.1:17451727-17451746 MS.gene35289:CDS 25.0%
!! AAAATGAGAAACAGTTGATA+GGG + chr2.1:17450262-17450281 None:intergenic 25.0%
!! GCTTTGATTTCATTATATGA+TGG + chr2.1:17451191-17451210 None:intergenic 25.0%
!!! GGGTTTTTAGTTAACAATAT+TGG - chr2.1:17450378-17450397 MS.gene35289:intron 25.0%
!!! TTAATTTGTGTGTTTTCTAC+CGG - chr2.1:17450167-17450186 MS.gene35289:CDS 25.0%
!!! TTGCATTGCTCTATTTTTTT+GGG + chr2.1:17451084-17451103 None:intergenic 25.0%
AAAGAAAAAGACATTCCCAA+AGG + chr2.1:17450842-17450861 None:intergenic 30.0%
AAGAAAAAGACATTCCCAAA+GGG + chr2.1:17450841-17450860 None:intergenic 30.0%
AATGAGATATCATCACAAGA+AGG - chr2.1:17449663-17449682 MS.gene35289:intron 30.0%
AATTCTAGTGAAGAAGGAAA+AGG - chr2.1:17449705-17449724 MS.gene35289:intron 30.0%
ATGAGATATCATCACAAGAA+GGG - chr2.1:17449664-17449683 MS.gene35289:intron 30.0%
ATGTTAAATCATGCATGTGA+TGG + chr2.1:17451035-17451054 None:intergenic 30.0%
ATTAACTGGAGAACTAACTT+GGG + chr2.1:17450422-17450441 None:intergenic 30.0%
ATTATGAATGTGCTAAACGT+TGG - chr2.1:17449961-17449980 MS.gene35289:intron 30.0%
ATTCAGAGATCAATATTGTC+GGG + chr2.1:17450111-17450130 None:intergenic 30.0%
ATTCTAGTGAAGAAGGAAAA+GGG - chr2.1:17449706-17449725 MS.gene35289:intron 30.0%
CAAAAAAATAGAGCAATGCA+AGG - chr2.1:17451083-17451102 MS.gene35289:intron 30.0%
CATCACATTATGGATATGTA+TGG - chr2.1:17451110-17451129 MS.gene35289:intron 30.0%
CTTATGAGTTCTGTATTTGT+TGG - chr2.1:17449908-17449927 MS.gene35289:intron 30.0%
GATATCATACCCAAACTTTA+GGG + chr2.1:17451382-17451401 None:intergenic 30.0%
TCCCAACTCATACTATAATA+TGG - chr2.1:17450234-17450253 MS.gene35289:intron 30.0%
TTTAGTGTAATTATAGGCGT+CGG + chr2.1:17450638-17450657 None:intergenic 30.0%
TTTGTAGATTTCATGAGAGT+GGG - chr2.1:17451648-17451667 MS.gene35289:CDS 30.0%
! GAACTCATAAGTTGTTTCAT+TGG + chr2.1:17449899-17449918 None:intergenic 30.0%
! TTTAGTACCCTAAAGTTTTG+TGG + chr2.1:17451467-17451486 None:intergenic 30.0%
! TTTTGTAGATTTCATGAGAG+TGG - chr2.1:17451647-17451666 MS.gene35289:CDS 30.0%
!! CTTTAGGAAGTAGTTCTTTT+TGG - chr2.1:17450136-17450155 MS.gene35289:CDS 30.0%
!! TAAGGTTCTGAAATATACCT+TGG + chr2.1:17450949-17450968 None:intergenic 30.0%
!!! CTTGCATTGCTCTATTTTTT+TGG + chr2.1:17451085-17451104 None:intergenic 30.0%
AAAATAGAGCAATGCAAGGA+AGG - chr2.1:17451087-17451106 MS.gene35289:intron 35.0%
AGTAAAAAGCTTGATGAGTC+TGG - chr2.1:17450923-17450942 MS.gene35289:intron 35.0%
ATGGATATGTATGGACACTT+TGG - chr2.1:17451119-17451138 MS.gene35289:intron 35.0%
CATTAACTGGAGAACTAACT+TGG + chr2.1:17450423-17450442 None:intergenic 35.0%
GGATATCATACCCAAACTTT+AGG + chr2.1:17451383-17451402 None:intergenic 35.0%
TACTTGGCTAGACCTAATAT+AGG + chr2.1:17451779-17451798 None:intergenic 35.0%
TCTCACATTATTAACGGTGT+CGG - chr2.1:17450669-17450688 MS.gene35289:intron 35.0%
TGAACAACACTTAGAAATCG+AGG - chr2.1:17450523-17450542 MS.gene35289:intron 35.0%
TTCAGAGATCAATATTGTCG+GGG + chr2.1:17450110-17450129 None:intergenic 35.0%
! AACGAACGTTTTCTTCGAAT+CGG + chr2.1:17449558-17449577 None:intergenic 35.0%
! ACTCATCAAGCTTTTTACTC+CGG + chr2.1:17450922-17450941 None:intergenic 35.0%
! CATGTGATAGTGATAAGTGA+AGG - chr2.1:17449618-17449637 MS.gene35289:intron 35.0%
! GATGTAGTATTTTCCTCTCT+TGG - chr2.1:17450039-17450058 MS.gene35289:intron 35.0%
! TATGATGGTTGTTTCAGTAG+AGG + chr2.1:17451176-17451195 None:intergenic 35.0%
! TGTTGCTTATTTCACCACAA+GGG + chr2.1:17449644-17449663 None:intergenic 35.0%
! TTGTTGCTTATTTCACCACA+AGG + chr2.1:17449645-17449664 None:intergenic 35.0%
! TTTTCTTCGAATCGGTAAAG+AGG + chr2.1:17449550-17449569 None:intergenic 35.0%
!!! AGTAGTTCTTTTTGGTGTCA+TGG - chr2.1:17450144-17450163 MS.gene35289:CDS 35.0%
AACCCTTGGAAACATCGAAT+AGG + chr2.1:17451008-17451027 None:intergenic 40.0%
AATATTGTCGGGGAGTTCAA+TGG + chr2.1:17450100-17450119 None:intergenic 40.0%
ACATGTGAAGAGTCATTTGC+AGG - chr2.1:17450466-17450485 MS.gene35289:intron 40.0%
ACCACATCAGTTAATGTCCA+TGG + chr2.1:17451304-17451323 None:intergenic 40.0%
ACTTCCGAACCCTAAAGTTT+GGG - chr2.1:17451370-17451389 MS.gene35289:intron 40.0%
AGTGAAAGTAGTAGCGGTAT+CGG - chr2.1:17449513-17449532 MS.gene35289:CDS 40.0%
AGTTGAGAAACATGCCCTTT+GGG - chr2.1:17450824-17450843 MS.gene35289:CDS 40.0%
CTCACATTATTAACGGTGTC+GGG - chr2.1:17450670-17450689 MS.gene35289:intron 40.0%
CTCCAGTTAATGAATGTGAG+AGG - chr2.1:17450431-17450450 MS.gene35289:intron 40.0%
CTTCAGATCAAGTTCAGTGT+GGG - chr2.1:17451694-17451713 MS.gene35289:CDS 40.0%
GCACATACACTCCTTTGATT+AGG - chr2.1:17450580-17450599 MS.gene35289:intron 40.0%
GTCAAGAGTGAAAGTAGTAG+CGG - chr2.1:17449507-17449526 MS.gene35289:CDS 40.0%
GTCCTCTCACATTCATTAAC+TGG + chr2.1:17450436-17450455 None:intergenic 40.0%
TCAAAGCTCATGACACTTCA+AGG - chr2.1:17451204-17451223 MS.gene35289:intron 40.0%
TCACTCTTGACATCTTTGCT+AGG + chr2.1:17449498-17449517 None:intergenic 40.0%
TCCATGGACATTAACTGATG+TGG - chr2.1:17451300-17451319 MS.gene35289:intron 40.0%
TCTTCAGATCAAGTTCAGTG+TGG - chr2.1:17451693-17451712 MS.gene35289:CDS 40.0%
TGCCTATTCGATGTTTCCAA+GGG - chr2.1:17451003-17451022 MS.gene35289:intron 40.0%
TGGACATTAACTGATGTGGT+TGG - chr2.1:17451304-17451323 MS.gene35289:intron 40.0%
! GTATGAGTTGGGACGTTAAA+AGG + chr2.1:17450227-17450246 None:intergenic 40.0%
! GTATTTTCCTCTCTTGGTCT+TGG - chr2.1:17450045-17450064 MS.gene35289:intron 40.0%
! TCACATTATTAACGGTGTCG+GGG - chr2.1:17450671-17450690 MS.gene35289:intron 40.0%
!! CATACCCAAACTTTAGGGTT+CGG + chr2.1:17451377-17451396 None:intergenic 40.0%
!! CATGTGATGGATAAAACCCT+TGG + chr2.1:17451022-17451041 None:intergenic 40.0%
!! TTTTGGAGGTTCAGATGTAC+CGG - chr2.1:17450900-17450919 MS.gene35289:intron 40.0%
ATGATCATCTCCATCGTCCA+CGG + chr2.1:17449601-17449620 None:intergenic 45.0%
ATGTGAGGTCGAAAATGCCT+AGG - chr2.1:17449751-17449770 MS.gene35289:intron 45.0%
CAATGTTCCAAGACCAAGAG+AGG + chr2.1:17450055-17450074 None:intergenic 45.0%
CACTTCCGAACCCTAAAGTT+TGG - chr2.1:17451369-17451388 MS.gene35289:intron 45.0%
CATTAATCAACCCCCTGAAC+TGG - chr2.1:17450191-17450210 MS.gene35289:intron 45.0%
CGATTTAAACGAAGAAGCCG+TGG - chr2.1:17449581-17449600 MS.gene35289:CDS 45.0%
GAGTTGAGAAACATGCCCTT+TGG - chr2.1:17450823-17450842 MS.gene35289:CDS 45.0%
GTACGGTTAGGCAATATGTG+AGG - chr2.1:17449736-17449755 MS.gene35289:intron 45.0%
GTGCCTATTCGATGTTTCCA+AGG - chr2.1:17451002-17451021 MS.gene35289:intron 45.0%
GTTTCAGTAGAGGAGACGAT+GGG + chr2.1:17451166-17451185 None:intergenic 45.0%
TGAAGCATGAGCACAAACAC+CGG + chr2.1:17450717-17450736 None:intergenic 45.0%
TGTTTCAGTAGAGGAGACGA+TGG + chr2.1:17451167-17451186 None:intergenic 45.0%
! CTTTTGTGCATGCTGTTGAG+AGG - chr2.1:17449802-17449821 MS.gene35289:intron 45.0%
! GTTGTTTCATTGGTCGCCAA+AGG + chr2.1:17449889-17449908 None:intergenic 45.0%
! TCAGGGGGTTGATTAATGTC+CGG + chr2.1:17450189-17450208 None:intergenic 45.0%
!! CTCTCTTGGTCTTGGAACAT+TGG - chr2.1:17450053-17450072 MS.gene35289:CDS 45.0%
!! GCAAGGAAGGCATCACATTA+TGG - chr2.1:17451100-17451119 MS.gene35289:intron 45.0%
!! GTAATTATAGGCGTCGGTGT+CGG + chr2.1:17450632-17450651 None:intergenic 45.0%
AAGCTTGATGAGTCTGGCCA+AGG - chr2.1:17450929-17450948 MS.gene35289:intron 50.0%
ACTGGGACACGCCTAATCAA+AGG + chr2.1:17450594-17450613 None:intergenic 50.0%
AGTGAAGAAGGAAAAGGGCG+TGG - chr2.1:17449711-17449730 MS.gene35289:intron 50.0%
AGTGTCAAGACCAGTTCAGG+GGG + chr2.1:17450204-17450223 None:intergenic 50.0%
ATGCTGTTGAGAGGCTTGGA+GGG - chr2.1:17449811-17449830 MS.gene35289:intron 50.0%
CGAAAATGCCTAGGCTTCGT+TGG - chr2.1:17449760-17449779 MS.gene35289:intron 50.0%
CTTCGAATCGGTAAAGAGGC+AGG + chr2.1:17449546-17449565 None:intergenic 50.0%
GAGTGTCAAGACCAGTTCAG+GGG + chr2.1:17450205-17450224 None:intergenic 50.0%
GTCAGTGTTTGCGTCGTATC+CGG - chr2.1:17450695-17450714 MS.gene35289:intron 50.0%
GTGATAAGTGAAGGCCCTTG+TGG - chr2.1:17449627-17449646 MS.gene35289:intron 50.0%
! AGGAAAAGGGCGTGGAAGTA+CGG - chr2.1:17449719-17449738 MS.gene35289:intron 50.0%
! GGAGTGTCAAGACCAGTTCA+GGG + chr2.1:17450206-17450225 None:intergenic 50.0%
!! AGGAGTGTCAAGACCAGTTC+AGG + chr2.1:17450207-17450226 None:intergenic 50.0%
AACGAAGAAGCCGTGGACGA+TGG - chr2.1:17449588-17449607 MS.gene35289:CDS 55.0%
CATGCTGTTGAGAGGCTTGG+AGG - chr2.1:17449810-17449829 MS.gene35289:intron 55.0%
GATTAGGCGTGTCCCAGTGT+TGG - chr2.1:17450596-17450615 MS.gene35289:intron 55.0%
GGACACACGTGTCCAACACT+GGG + chr2.1:17450611-17450630 None:intergenic 55.0%
GTGCATGCTGTTGAGAGGCT+TGG - chr2.1:17449807-17449826 MS.gene35289:intron 55.0%
TCAGGAGTCCAACGAAGCCT+AGG + chr2.1:17449771-17449790 None:intergenic 55.0%
! AAGGGCGTGGAAGTACGGTT+AGG - chr2.1:17449724-17449743 MS.gene35289:intron 55.0%
CGGACACACGTGTCCAACAC+TGG + chr2.1:17450612-17450631 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 17449477 17451799 17449477 ID=MS.gene35289
chr2.1 mRNA 17449477 17451799 17449477 ID=MS.gene35289.t1;Parent=MS.gene35289
chr2.1 exon 17451436 17451799 17451436 ID=MS.gene35289.t1.exon1;Parent=MS.gene35289.t1
chr2.1 CDS 17451436 17451799 17451436 ID=cds.MS.gene35289.t1;Parent=MS.gene35289.t1
chr2.1 exon 17450789 17450865 17450789 ID=MS.gene35289.t1.exon2;Parent=MS.gene35289.t1
chr2.1 CDS 17450789 17450865 17450789 ID=cds.MS.gene35289.t1;Parent=MS.gene35289.t1
chr2.1 exon 17450326 17450362 17450326 ID=MS.gene35289.t1.exon3;Parent=MS.gene35289.t1
chr2.1 CDS 17450326 17450362 17450326 ID=cds.MS.gene35289.t1;Parent=MS.gene35289.t1
chr2.1 exon 17450051 17450201 17450051 ID=MS.gene35289.t1.exon4;Parent=MS.gene35289.t1
chr2.1 CDS 17450051 17450201 17450051 ID=cds.MS.gene35289.t1;Parent=MS.gene35289.t1
chr2.1 exon 17449477 17449621 17449477 ID=MS.gene35289.t1.exon5;Parent=MS.gene35289.t1
chr2.1 CDS 17449477 17449621 17449477 ID=cds.MS.gene35289.t1;Parent=MS.gene35289.t1
Gene Sequence

>MS.gene35289

ATGGCGGAGATTCACATACCTAGCAAAGATGTCAAGAGTGAAAGTAGTAGCGGTATCGGAGCAAAACCTGCCTCTTTACCGATTCGAAGAAAACGTTCGTTCTTCGATTTAAACGAAGAAGCCGTGGACGATGGAGATGATCATGTGATAGTGATAAGTGAAGGCCCTTGTGGTGAAATAAGCAACAATGAGATATCATCACAAGAAGGGAATTTGAGTAGCAATAATAATTCTAGTGAAGAAGGAAAAGGGCGTGGAAGTACGGTTAGGCAATATGTGAGGTCGAAAATGCCTAGGCTTCGTTGGACTCCTGATCTTCATTTATCTTTTGTGCATGCTGTTGAGAGGCTTGGAGGGCAAGAAAGTAAGTGTGACTCATTTCATTCTTATGAACATAAATTTATCATTACCTTTGGCGACCAATGAAACAACTTATGAGTTCTGTATTTGTTGGGTTCAAGTGTGAGCGATTAGTCATACATCAATTATGAATGTGCTAAACGTTGGATATATAAGATCGAAAGATGACTTATATACCTAATGTATTACGATTCTGAATGGAGATGTAGTATTTTCCTCTCTTGGTCTTGGAACATTGGTCATTTGTTGTTCTCGATGCTCCATTGAACTCCCCGACAATATTGATCTCTGAATTATATCTTTAGGAAGTAGTTCTTTTTGGTGTCATGGTTAATTTGTGTGTTTTCTACCGGACATTAATCAACCCCCTGAACTGGTCTTGACACTCCTTTTAACGTCCCAACTCATACTATAATATGGATCCCTATCAACTGTTTCTCATTTTTGTAATTTCTTTGTTTATGAGTTGAACTACTATTTACACTTAATCTTATATAGATATGGATTTGCATTATTCTAAAATTTGATGTATTTATTTTGAGGGTTTTTAGTTAACAATATTGGATATATGTAGGAGCAACACCCAAGTTAGTTCTCCAGTTAATGAATGTGAGAGGACTTAGCATTGCACATGTGAAGAGTCATTTGCAGGTAATATATATTCTTGTATAGGAGAAGTTGATTTATGAACAACACTTAGAAATCGAGGTTACACAATTTATTGCTTAATTAACTGTTATGAAGCACATACACTCCTTTGATTAGGCGTGTCCCAGTGTTGGACACGTGTGTCCGACACCGACGCCTATAATTACACTAAATTATGTGATTTTCTCACATTATTAACGGTGTCGGGGTGTCAGTGTTTGCGTCGTATCCGGTGTTTGTGCTCATGCTTCATAGATTAATTGTGTTTGTAATGGGTTAGAGTATCTCTTTTATTCTTTTATTTTTGTGTCACTTTTGCGAATACAAAATACTATGTTGAGTTGAGAAACATGCCCTTTGGGAATGTCTTTTTCTTTTGTATTCTATCTTTCTTATAACGTTTTAATTATGATTTTTTTGGAGGTTCAGATGTACCGGAGTAAAAAGCTTGATGAGTCTGGCCAAGGTATATTTCAGAACCTTAATATTGTTAGCAAAATCATGTTTTATACGAAGTGTGCCTATTCGATGTTTCCAAGGGTTTTATCCATCACATGCATGATTTAACATTCATTTTTGTTTTATTTCAGTTTTGTCCCAAAAAAATAGAGCAATGCAAGGAAGGCATCACATTATGGATATGTATGGACACTTTGGTGTTGAAAGCAACTATTTGCACCCATCGTCTCCTCTACTGAAACAACCATCATATAATGAAATCAAAGCTCATGACACTTCAAGGTATAGTATCTAAAAAAAAACTTTAAGATTATTTATATTTTTATGCACTACAAATGTAAAAAATAAAATAAAATTCCATGGACATTAACTGATGTGGTTGGAATACAACATTAAATATGTCTGTAAAATTATATTTTGATGTACACTTCCGAACCCTAAAGTTTGGGTATGATATCCCAACATTCAATCATTTAATGTCATATTTTTTTTTATATTTAACTATTTTAAATTAATTTTACCACAAAACTTTAGGGTACTAAAGCATTTCCCAAATATTTTGCACTATTACTAATCTAAAACAATACAATGAAAACCATTACATATTTTTCTTATATAATTATTATAGTGAAAATTTGAAATAAAATAGAAACATTTAAAACATGGATATGAAATTAATTTTGATAATTCTTTAATGTGTTAATTTTGTAGATTTCATGAGAGTGGGCTTTTCAATAATAATCACGTGATCTTCAGATCAAGTTCAGTGTGGGACAAAGATTTTTCACATCTATTTGATGTAAAGGATGCAATTACAAGAAATAATATTGCACCTATATTAGGTCTAGCCAAGTAA
Protein sequence

>MS.gene35289.t1

MAEIHIPSKDVKSESSSGIGAKPASLPIRRKRSFFDLNEEAVDDGDDHVIVISEGPCGEISNNEISSQEGNLSSNNNSSEEGKGRGSTVRQYVRSKMPRLRWTPDLHLSFVHAVERLGGQERATPKLVLQLMNVRGLSIAHVKSHLQMYRSKKLDESGQVLSQKNRAMQGRHHIMDMYGHFGVESNYLHPSSPLLKQPSYNEIKAHDTSRFHESGLFNNNHVIFRSSSVWDKDFSHLFDVKDAITRNNIAPILGLAK