AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35568

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35568.t1 MTR_3g074930 92.208 616 47 1 4 619 8 622 0.0 1208
MS.gene35568.t1 MTR_3g074930 91.172 623 47 2 4 619 8 629 0.0 1202
MS.gene35568.t1 MTR_4g103520 79.441 608 119 2 14 619 23 626 0.0 1041
MS.gene35568.t1 MTR_4g103520 81.193 553 98 2 69 619 1 549 0.0 964
MS.gene35568.t1 MTR_5g045600 70.230 608 176 2 14 619 14 618 0.0 909
MS.gene35568.t1 MTR_8g063080 68.943 615 184 2 5 619 4 611 0.0 909
MS.gene35568.t1 MTR_3g074970 84.713 471 27 2 148 618 100 525 0.0 822
MS.gene35568.t1 MTR_3g074970 95.833 96 4 0 517 612 3 98 1.89e-54 194
MS.gene35568.t1 MTR_3g074960 72.154 492 72 5 4 474 8 455 0.0 706
MS.gene35568.t1 MTR_4g037670 35.640 578 312 18 61 600 79 634 6.97e-107 336
MS.gene35568.t1 MTR_1g052505 35.000 560 309 16 78 600 74 615 3.91e-103 325
MS.gene35568.t1 MTR_4g082930 28.454 608 352 23 42 598 5 580 3.20e-58 207
MS.gene35568.t1 MTR_4g082940 27.632 608 346 23 40 598 4 566 7.57e-52 189
MS.gene35568.t1 MTR_6g005650 82.051 78 14 0 189 266 10 87 2.15e-37 141
MS.gene35568.t1 MTR_1g060360 27.229 415 228 15 189 593 57 407 1.06e-29 122
MS.gene35568.t1 MTR_7g104330 25.908 413 228 15 189 589 65 411 5.37e-29 120
MS.gene35568.t1 MTR_4g115670 26.190 462 277 16 160 602 35 451 9.53e-28 117
MS.gene35568.t1 MTR_7g104360 25.791 411 232 13 189 589 58 405 1.52e-27 115
MS.gene35568.t1 MTR_3g051240 26.218 431 246 14 189 613 54 418 2.91e-26 112
MS.gene35568.t1 MTR_6g013050 27.053 414 186 15 246 589 146 513 1.64e-25 111
MS.gene35568.t1 MTR_6g013050 27.204 397 173 15 246 572 146 496 7.60e-23 103
MS.gene35568.t1 MTR_5g092360 24.390 451 263 18 173 613 42 424 1.50e-22 101
MS.gene35568.t1 MTR_7g056403 25.352 426 202 13 246 600 146 526 2.37e-22 101
MS.gene35568.t1 MTR_3g083810 24.186 430 202 14 246 600 147 527 8.06e-22 100
MS.gene35568.t1 MTR_3g083820 23.776 429 205 14 246 600 147 527 1.59e-20 95.9
MS.gene35568.t1 MTR_0188s0020 24.796 367 223 13 246 595 119 449 4.24e-20 94.0
MS.gene35568.t1 MTR_1g112630 24.122 427 242 19 190 590 70 440 3.57e-18 88.2
MS.gene35568.t1 MTR_4g037105 24.793 363 218 15 246 590 113 438 5.42e-18 87.4
MS.gene35568.t1 MTR_7g405800 93.333 30 2 0 232 261 1 30 6.31e-14 72.0
MS.gene35568.t1 MTR_2g020270 68.085 47 13 1 119 163 33 79 1.01e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35568.t1 AT5G50400 73.322 611 157 3 9 619 6 610 0.0 949
MS.gene35568.t1 AT4G24890 72.120 599 164 2 21 619 19 614 0.0 922
MS.gene35568.t1 AT1G13750 66.667 615 202 1 5 619 1 612 0.0 876
MS.gene35568.t1 AT5G50400 78.865 511 107 1 109 619 29 538 0.0 865
MS.gene35568.t1 AT1G13900 29.568 602 323 26 50 598 24 577 1.58e-58 208
MS.gene35568.t1 AT2G03450 28.642 611 333 25 43 598 15 577 2.16e-57 205
MS.gene35568.t1 AT3G52780 26.218 431 244 15 172 589 36 405 1.85e-28 119
MS.gene35568.t1 AT3G52810 26.450 431 243 17 169 589 42 408 1.37e-27 116
MS.gene35568.t1 AT3G52820 26.728 434 248 18 162 589 35 404 3.69e-24 106
MS.gene35568.t1 AT4G13700 26.970 330 171 11 246 547 140 427 6.12e-24 105
MS.gene35568.t1 AT3G20500 30.466 279 163 10 320 589 147 403 6.91e-24 105
MS.gene35568.t1 AT2G27190 27.671 365 205 15 246 590 118 443 9.68e-23 102
MS.gene35568.t1 AT3G52780 27.507 349 198 12 172 513 36 336 1.50e-22 100
MS.gene35568.t1 AT5G34850 25.203 369 234 12 246 602 112 450 2.23e-22 101
MS.gene35568.t1 AT4G36350 24.044 366 218 13 246 591 114 439 1.29e-19 92.8
MS.gene35568.t1 AT3G07130 24.466 421 209 15 246 600 140 517 2.75e-19 92.0
MS.gene35568.t1 AT4G13700 26.804 291 153 10 246 508 140 398 5.53e-19 90.1
MS.gene35568.t1 AT1G56360 22.782 417 264 16 191 591 65 439 9.79e-19 89.7
MS.gene35568.t1 AT2G18130 25.714 280 179 10 338 602 168 433 4.29e-15 78.6
MS.gene35568.t1 AT2G32770 22.170 424 201 16 246 589 149 523 7.76e-15 78.2
MS.gene35568.t1 AT3G07130 22.676 441 225 16 246 600 140 550 1.06e-14 77.8
MS.gene35568.t1 AT2G32770 23.343 347 172 14 246 547 68 365 7.99e-13 71.2

Find 167 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTTCATTTACCTTTAT+TGG 0.162930 3.3:-64571680 None:intergenic
TTTAATGCACACCTCATATT+TGG 0.186772 3.3:-64572844 None:intergenic
TAGTAGTTATACCTGATAAA+TGG 0.220072 3.3:-64574669 None:intergenic
GAGTTTCATTTACCTTTATT+GGG 0.220701 3.3:-64571679 None:intergenic
CAGTCTTCATTAGTTGGTTC+TGG 0.230728 3.3:-64570234 None:intergenic
TACCTTACTGCCAAGCAAAA+AGG 0.234836 3.3:-64568177 None:intergenic
TAATATAACTCACCAGAATT+TGG 0.238636 3.3:-64573644 None:intergenic
TGAATCTGCTTTCCACCCTT+TGG 0.258361 3.3:-64572609 None:intergenic
TGCAGGTATGCCACAGATTA+TGG 0.265751 3.3:+64574365 MS.gene35568:intron
GAATCTGCTTTCCACCCTTT+GGG 0.297542 3.3:-64572608 None:intergenic
GCGAACCTTGCTATCATATA+TGG 0.299475 3.3:-64573414 None:intergenic
GGGAAAGTTTGCAGGGGCTT+TGG 0.317516 3.3:+64574585 MS.gene35568:CDS
AGAGACAAAGACTATGGTTT+TGG 0.320936 3.3:+64574933 MS.gene35568:CDS
CATGGGAAGGGAAAGTTTGC+AGG 0.323081 3.3:+64574577 MS.gene35568:CDS
TTCTATGACACTCCAGATTC+AGG 0.324023 3.3:+64573565 MS.gene35568:CDS
CGCGGATACACAGGTGCTTT+AGG 0.325055 3.3:-64572335 None:intergenic
CTGCACGTTGATTGATCAAT+TGG 0.331585 3.3:-64571856 None:intergenic
CCGCATTCTCCACCTGAATC+TGG 0.338489 3.3:-64573577 None:intergenic
TTGGGTGATATGAATGGATT+TGG 0.345669 3.3:+64568077 MS.gene35568:CDS
AAGAGTATGCCCCATTTATC+AGG 0.358857 3.3:+64574658 MS.gene35568:CDS
TTGCAACGTGTTATCATATT+CGG 0.359394 3.3:+64573044 MS.gene35568:CDS
CCCAGGTACGCCTATAGGTT+GGG 0.360573 3.3:+64572942 MS.gene35568:intron
CACCAACTTGAGGATCTGTT+TGG 0.360863 3.3:+64568058 MS.gene35568:CDS
AGTAGTTATACCTGATAAAT+GGG 0.363474 3.3:-64574668 None:intergenic
AGTCTTCATTAGTTGGTTCT+GGG 0.366107 3.3:-64570233 None:intergenic
CGTCCAATGCCACCGGGGTT+GGG 0.366293 3.3:-64571629 None:intergenic
ACCGTTGGCTGGCGCGATCC+TGG 0.370934 3.3:+64572787 MS.gene35568:CDS
GGCATACTCTTTCGTAGTTA+TGG 0.374674 3.3:-64574646 None:intergenic
CTGCAAACTTTCCCTTCCCA+TGG 0.377219 3.3:-64574576 None:intergenic
CATTATTCTGGCACTGTGAA+TGG 0.377604 3.3:+64574831 MS.gene35568:CDS
TCACCACAGCACCTCCTGTT+TGG 0.380045 3.3:+64574901 MS.gene35568:CDS
AGCTGTACCATTTGTTGAAT+GGG 0.384608 3.3:+64572584 MS.gene35568:CDS
GTATGTTACTTGAAGTTTGC+AGG 0.390132 3.3:-64570273 None:intergenic
CAGGTTTCCTTGAAGAACTT+TGG 0.392296 3.3:+64572821 MS.gene35568:CDS
CCGTCCAATGCCACCGGGGT+TGG 0.396303 3.3:-64571630 None:intergenic
AGGTAAGTTAAACATGAGTT+TGG 0.397660 3.3:+64568016 MS.gene35568:CDS
CTGAGAACAAAGCCAAATTC+TGG 0.397715 3.3:+64573632 MS.gene35568:CDS
AGAATGGGTGACAGTAAAAC+TGG 0.399637 3.3:+64570206 MS.gene35568:CDS
CATGTTGTTGTTGGTGGAGG+GGG 0.399824 3.3:+64574861 MS.gene35568:CDS
TGTCTGATGGCTCTTATGTA+TGG 0.401100 3.3:+64572973 MS.gene35568:CDS
CATATGCTCAGCCCCAATAA+AGG 0.402084 3.3:+64571667 MS.gene35568:CDS
TCATAGAAGGATCCTGAGTT+TGG 0.403373 3.3:-64573550 None:intergenic
CAATAAATGCCCCAACCTAT+AGG 0.409594 3.3:-64572952 None:intergenic
TCTTCCCCAGGTACGCCTAT+AGG 0.412213 3.3:+64572937 MS.gene35568:intron
CCAACCCAGTCTTCATTAGT+TGG 0.419485 3.3:-64570240 None:intergenic
ATCTCTATAGTCCCTGGAAA+TGG 0.436173 3.3:-64575036 None:intergenic
TTCAAGGCATCTCCTTATCC+TGG 0.438321 3.3:+64573011 MS.gene35568:CDS
AAGAACTTTGGCCAAATATG+AGG 0.438770 3.3:+64572833 MS.gene35568:CDS
AATTTGGCTTTGTTCTCAGC+AGG 0.439009 3.3:-64573628 None:intergenic
CTTGGCATACCAATCATTAG+AGG 0.440011 3.3:-64574534 None:intergenic
CCACCCAACCCCGGTGGCAT+TGG 0.440027 3.3:+64571626 MS.gene35568:CDS
AACCTGCGCGAACCGTTGGC+TGG 0.449374 3.3:+64572776 MS.gene35568:CDS
TGAATCTGGAGTGTCATAGA+AGG 0.450964 3.3:-64573563 None:intergenic
CTCTTCAAATGAACCCTCCT+TGG 0.453099 3.3:-64574552 None:intergenic
AAGCAGATTCAATCTGCTGC+TGG 0.454182 3.3:+64572621 MS.gene35568:CDS
TTTGGGCCCCATTCAACAAA+TGG 0.461006 3.3:-64572591 None:intergenic
ACCAACTTGAGGATCTGTTT+GGG 0.461280 3.3:+64568059 MS.gene35568:CDS
TATTTGGCCAAAGTTCTTCA+AGG 0.461537 3.3:-64572828 None:intergenic
GGAACAATTCATGTTGTTGT+TGG 0.462326 3.3:+64574852 MS.gene35568:CDS
GTGGAAATCACGAACGCTCT+TGG 0.464456 3.3:+64573527 MS.gene35568:intron
CTCAGCAGGGTAATAGTACA+TGG 0.467918 3.3:-64573614 None:intergenic
ATCTGTTTGGGTGATATGAA+TGG 0.471658 3.3:+64568071 MS.gene35568:CDS
ATTCATGTTGTTGTTGGTGG+AGG 0.474320 3.3:+64574858 MS.gene35568:CDS
TTGGGGCATTTATTGTCTGA+TGG 0.475270 3.3:+64572960 MS.gene35568:CDS
ATCTACCCGTCCAATGCCAC+CGG 0.476003 3.3:-64571636 None:intergenic
ATTGGGGCTGAGCATATGAA+TGG 0.477674 3.3:-64571662 None:intergenic
CCCCAGGTACGCCTATAGGT+TGG 0.480019 3.3:+64572941 MS.gene35568:intron
TCTCTGAAAATACTCCAAAC+AGG 0.481381 3.3:-64574915 None:intergenic
CAGGGACTATAGAGATGTCT+TGG 0.482107 3.3:+64575043 MS.gene35568:CDS
GTATTTAGGATTGGAGTGGT+TGG 0.482977 3.3:-64571804 None:intergenic
GTGTATCCGCGCCTTGCTCA+TGG 0.485506 3.3:+64572347 MS.gene35568:CDS
AAGCTGTACCATTTGTTGAA+TGG 0.486572 3.3:+64572583 MS.gene35568:CDS
TTCATGTTGTTGTTGGTGGA+GGG 0.486891 3.3:+64574859 MS.gene35568:CDS
TTTGAAACTGCAACAAGCTT+AGG 0.488913 3.3:-64572293 None:intergenic
TCGGTTACTCCTCTAATGAT+TGG 0.490311 3.3:+64574525 MS.gene35568:CDS
TGTCTAAATCCTTGATGAGC+TGG 0.492936 3.3:-64573287 None:intergenic
GCTCTTATGTATGGAGTAAG+AGG 0.494084 3.3:+64572982 MS.gene35568:CDS
ACAATTCATGTTGTTGTTGG+TGG 0.495571 3.3:+64574855 MS.gene35568:CDS
CAGAACCAACTAATGAAGAC+TGG 0.498767 3.3:+64570235 MS.gene35568:CDS
CGCCAGCCAACGGTTCGCGC+AGG 0.499949 3.3:-64572778 None:intergenic
TGCAAACTTTCCCTTCCCAT+GGG 0.500947 3.3:-64574575 None:intergenic
AGAATCATGGATCGCAAGAA+TGG 0.504874 3.3:-64568135 None:intergenic
GGAGATCTGCCATATGCAAA+TGG 0.505321 3.3:+64573333 MS.gene35568:CDS
TTTGTTGAATGGGGCCCAAA+GGG 0.507205 3.3:+64572594 MS.gene35568:CDS
AGTTTCATTTACCTTTATTG+GGG 0.515764 3.3:-64571678 None:intergenic
CCAACCCCGGTGGCATTGGA+CGG 0.516793 3.3:+64571630 MS.gene35568:CDS
GAGTAAGAGGTATTCATTCA+AGG 0.520098 3.3:+64572995 MS.gene35568:CDS
GGTCAACCTGCGCGAACCGT+TGG 0.521129 3.3:+64572772 MS.gene35568:intron
CAACCCCGGTGGCATTGGAC+GGG 0.532592 3.3:+64571631 MS.gene35568:CDS
TGAGCAAGGCGCGGATACAC+AGG 0.538957 3.3:-64572344 None:intergenic
GAACGCTCTTGGCCAAACTC+AGG 0.539098 3.3:+64573538 MS.gene35568:CDS
CAGTAACGGAGGCAGAATCA+TGG 0.541508 3.3:-64568148 None:intergenic
TAATAGTACATGGTTTCTGC+AGG 0.541874 3.3:-64573604 None:intergenic
CAAGAAAAGTAGTGATGGAA+AGG 0.542299 3.3:+64574998 MS.gene35568:CDS
TCTACCCGTCCAATGCCACC+GGG 0.543273 3.3:-64571635 None:intergenic
GTCCTTTAGGCTGAGCGTGA+TGG 0.547365 3.3:+64573216 MS.gene35568:intron
GTCTTCATTAGTTGGTTCTG+GGG 0.549003 3.3:-64570232 None:intergenic
TCCAAGACTTTCCATGAGCA+AGG 0.549311 3.3:-64572358 None:intergenic
TCATGTTGTTGTTGGTGGAG+GGG 0.552640 3.3:+64574860 MS.gene35568:CDS
TATGACAGATGACAGTTACC+TGG 0.552868 3.3:+64572538 MS.gene35568:intron
TGTTATCATATTCGGTGACA+TGG 0.552892 3.3:+64573052 MS.gene35568:CDS
CCAATGCCACCGGGGTTGGG+TGG 0.553233 3.3:-64571626 None:intergenic
GGGTTCATTTGAAGAGCCCA+TGG 0.554005 3.3:+64574559 MS.gene35568:CDS
CTCTAATGATTGGTATGCCA+AGG 0.554950 3.3:+64574535 MS.gene35568:CDS
CCAGGTACGCCTATAGGTTG+GGG 0.555647 3.3:+64572943 MS.gene35568:intron
ATTTGTTGAATGGGGCCCAA+AGG 0.559998 3.3:+64572593 MS.gene35568:CDS
TAGCAGACACTGAACATGAC+TGG 0.560714 3.3:+64574405 MS.gene35568:CDS
GCAGGGCGAGGATACAGAAT+GGG 0.561327 3.3:+64570191 MS.gene35568:intron
TATGTCATATCCACTTGTCC+AGG 0.562397 3.3:-64572556 None:intergenic
GCCTCTTCATCACCAACTTG+AGG 0.562952 3.3:+64568048 MS.gene35568:CDS
TCCTCAAGTTGGTGATGAAG+AGG 0.564410 3.3:-64568049 None:intergenic
ATTTGAAGAGCCCATGGGAA+GGG 0.565074 3.3:+64574565 MS.gene35568:CDS
GAGATGTATCCATTTGCATA+TGG 0.566529 3.3:-64573342 None:intergenic
CAAGACATCTCTATAGTCCC+TGG 0.567071 3.3:-64575042 None:intergenic
GAATATGCTAATTATCAACC+TGG 0.571971 3.3:+64573246 MS.gene35568:CDS
ACTGAACATGACTGGAGAGA+AGG 0.580541 3.3:+64574413 MS.gene35568:CDS
CACCACTGACCAGCTCATCA+AGG 0.584625 3.3:+64573278 MS.gene35568:CDS
ACCAGGATCGCGCCAGCCAA+CGG 0.585321 3.3:-64572788 None:intergenic
GAGTACAAGAAAAGTAGTGA+TGG 0.585752 3.3:+64574993 MS.gene35568:CDS
TGCAGGGCGAGGATACAGAA+TGG 0.587032 3.3:+64570190 MS.gene35568:intron
CATATTCGGTGACATGGGAA+AGG 0.587852 3.3:+64573058 MS.gene35568:CDS
ATGACAGTTACCTGGACAAG+TGG 0.589057 3.3:+64572546 MS.gene35568:CDS
ACCCAAACAGATCCTCAAGT+TGG 0.593043 3.3:-64568060 None:intergenic
CAGTTCCATATATGATAGCA+AGG 0.595584 3.3:+64573409 MS.gene35568:CDS
ATGGGAAGGGAAAGTTTGCA+GGG 0.596275 3.3:+64574578 MS.gene35568:CDS
CCAACTAATGAAGACTGGGT+TGG 0.597652 3.3:+64570240 MS.gene35568:CDS
TCCACCTGAAAATAGTGCAA+AGG 0.604263 3.3:-64571885 None:intergenic
GTTGAATGGGGCCCAAAGGG+TGG 0.605971 3.3:+64572597 MS.gene35568:CDS
TTTGCAGCACATCGCCCACT+CGG 0.606514 3.3:+64574506 MS.gene35568:CDS
TGAATGGCAATCTTTGAAAG+TGG 0.607314 3.3:-64568107 None:intergenic
CATTTGAAGAGCCCATGGGA+AGG 0.608262 3.3:+64574564 MS.gene35568:CDS
CATATGGCAGATCTCCTATG+TGG 0.608314 3.3:-64573326 None:intergenic
GCCTTGCTCATGGAAAGTCT+TGG 0.610045 3.3:+64572357 MS.gene35568:CDS
AAACCTGTGTGTATAAAACC+AGG 0.612380 3.3:-64572805 None:intergenic
GTTATCATATTCGGTGACAT+GGG 0.619175 3.3:+64573053 MS.gene35568:CDS
ACATGACTGGAGAGAAGGAT+CGG 0.620114 3.3:+64574418 MS.gene35568:CDS
CCAGATTCAGGTGGAGAATG+CGG 0.625344 3.3:+64573577 MS.gene35568:CDS
AATGATTGGTATGCCAAGGA+GGG 0.625443 3.3:+64574539 MS.gene35568:CDS
ATTTGGCTTTGTTCTCAGCA+GGG 0.626371 3.3:-64573627 None:intergenic
CTCACAACCATCGTGCACAC+AGG 0.626678 3.3:-64575066 None:intergenic
ACTCCAAACAGGAGGTGCTG+TGG 0.627083 3.3:-64574904 None:intergenic
TAATGATTGGTATGCCAAGG+AGG 0.627561 3.3:+64574538 MS.gene35568:CDS
AGAACCAACTAATGAAGACT+GGG 0.631293 3.3:+64570236 MS.gene35568:CDS
ATCCTTGATGAGCTGGTCAG+TGG 0.633150 3.3:-64573280 None:intergenic
GGTTCATTTGAAGAGCCCAT+GGG 0.633987 3.3:+64574560 MS.gene35568:CDS
GACTTTCCATGAGCAAGGCG+CGG 0.635264 3.3:-64572353 None:intergenic
TCAGTGGTGTTAAGTGAACC+AGG 0.637131 3.3:-64573264 None:intergenic
TTTAATCGTAACAGCATGTG+TGG 0.639129 3.3:+64572654 MS.gene35568:CDS
TCAACATGTCCACCCAACCC+CGG 0.639236 3.3:+64571617 MS.gene35568:CDS
TATGACACTCCAGATTCAGG+TGG 0.641435 3.3:+64573568 MS.gene35568:CDS
CATAATTCAGAGTGCCAAGG+TGG 0.647479 3.3:-64575093 None:intergenic
GCTGTACCATTTGTTGAATG+GGG 0.649227 3.3:+64572585 MS.gene35568:CDS
ACGAAACATGCCATAATCTG+TGG 0.650821 3.3:-64574375 None:intergenic
TAGATAGAAAACAACAACCA+TGG 0.654457 3.3:+64574477 MS.gene35568:CDS
AAATGGATACATCTCACAGT+GGG 0.656289 3.3:+64573350 MS.gene35568:CDS
CTACATAATTCAGAGTGCCA+AGG 0.658085 3.3:-64575096 None:intergenic
GTCTTGGCCTGTGTGCACGA+TGG 0.663436 3.3:+64575059 MS.gene35568:CDS
GTAGTTATACCTGATAAATG+GGG 0.675933 3.3:-64574667 None:intergenic
CATTAGAGGAGTAACCGAGT+GGG 0.692825 3.3:-64574520 None:intergenic
CTGAAAATACTCCAAACAGG+AGG 0.700173 3.3:-64574912 None:intergenic
TGGGAAGGGAAAGTTTGCAG+GGG 0.702638 3.3:+64574579 MS.gene35568:CDS
TCATTAGAGGAGTAACCGAG+TGG 0.714971 3.3:-64574521 None:intergenic
CTACCCGTCCAATGCCACCG+GGG 0.719235 3.3:-64571634 None:intergenic
TGGTTGTGAGAAAACCACCT+TGG 0.719747 3.3:+64575079 MS.gene35568:CDS
ACATGTCCACCCAACCCCGG+TGG 0.724016 3.3:+64571620 MS.gene35568:CDS
CAAATGGATACATCTCACAG+TGG 0.732996 3.3:+64573349 MS.gene35568:CDS
TGTGCTGCAAAGATTAACCA+TGG 0.735965 3.3:-64574494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.3 gene 64568011 64575109 64568011 ID=MS.gene35568
chr3.3 mRNA 64568011 64575109 64568011 ID=MS.gene35568.t1;Parent=MS.gene35568
chr3.3 exon 64568011 64568196 64568011 ID=MS.gene35568.t1.exon1;Parent=MS.gene35568.t1
chr3.3 CDS 64568011 64568196 64568011 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64570195 64570286 64570195 ID=MS.gene35568.t1.exon2;Parent=MS.gene35568.t1
chr3.3 CDS 64570195 64570286 64570195 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64571613 64571688 64571613 ID=MS.gene35568.t1.exon3;Parent=MS.gene35568.t1
chr3.3 CDS 64571613 64571688 64571613 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64571794 64571916 64571794 ID=MS.gene35568.t1.exon4;Parent=MS.gene35568.t1
chr3.3 CDS 64571794 64571916 64571794 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64572293 64572385 64572293 ID=MS.gene35568.t1.exon5;Parent=MS.gene35568.t1
chr3.3 CDS 64572293 64572385 64572293 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64572546 64572675 64572546 ID=MS.gene35568.t1.exon6;Parent=MS.gene35568.t1
chr3.3 CDS 64572546 64572675 64572546 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64572773 64572854 64572773 ID=MS.gene35568.t1.exon7;Parent=MS.gene35568.t1
chr3.3 CDS 64572773 64572854 64572773 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64572947 64573079 64572947 ID=MS.gene35568.t1.exon8;Parent=MS.gene35568.t1
chr3.3 CDS 64572947 64573079 64572947 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64573225 64573430 64573225 ID=MS.gene35568.t1.exon9;Parent=MS.gene35568.t1
chr3.3 CDS 64573225 64573430 64573225 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64573528 64573653 64573528 ID=MS.gene35568.t1.exon10;Parent=MS.gene35568.t1
chr3.3 CDS 64573528 64573653 64573528 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64574370 64574679 64574370 ID=MS.gene35568.t1.exon11;Parent=MS.gene35568.t1
chr3.3 CDS 64574370 64574679 64574370 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
chr3.3 exon 64574804 64575109 64574804 ID=MS.gene35568.t1.exon12;Parent=MS.gene35568.t1
chr3.3 CDS 64574804 64575109 64574804 ID=cds.MS.gene35568.t1;Parent=MS.gene35568.t1
Gene Sequence

>MS.gene35568

ATGGAAGGTAAGTTAAACATGAGTTTGGTAATATTGTGCCTCTTCATCACCAACTTGAGGATCTGTTTGGGTGATATGAATGGATTTGGAGAGCAGCCACTTTCAAAGATTGCCATTCACAAAACCATTCTTGCGATCCATGATTCTGCCTCCGTTACTGCTTCCCCCTTTTTGCTTGGCAGTAAGGTAGTATCTTCTACCCCGTTTCATTAATTTAACCTATTTATTTTGTTCTCACTAGCATGACATTAACATGCATGTTTTTCCACTTTTATTAGCAATGAATGATAATATGTTACAAAACAATTAAGTGAAATGTTAAATTGGAAGAAAAAATGTTACACCAAAATAGTTAAGTAATTCATTAATAGAAATTTTGTCTTGTAAATATAAGCTTTTACTTTTGTCTTAGTAATAATCACACCATTTTTTGATACAAACTTACTAATTTTAACACTTTAACCAATATCTCAAGGGCATTCATTAGCATTCTCCTTCCTTTATATATTGATATAGATTTATCAACAAATGTTTTGATTAAAAAAAATAACTATATTATTTTTTGAGAGAACTAATGAAATTTAGTTTTTTGAGGGGAGAATTAATGAAAGTTAAATAGTACTTGTTTCCCTCAACAAAACAAAACAGAAAATAGTACTTGATCAAGACATTTTATTTCTGCACAACTGCACCCCTTGATTTTACTTTCTACACCTGTTTGAGGCGATTTGAGCTTGGTATTTTTCTCCAGCCAGAGAAAAACGATACAATTACTGTAATTTTAGCTAGTACTAGCATGAAAATAGTTTTTTTTTTTTAGAGAAGCATGAAAATAGTTTAAAAGATGCAAAATTGTTTGCATGTAGCTCATAAATCTTTTATATGTTCGGTTTTCGTTGATTAGTGTCACATTTATTAAAATAACTATAATTTAAAATAAATCTTGTTTTTAATATTTTATAGGCTTAAATGCGCTTTTGGTCCCTTAACTATTTATTTGATATCGCTTTGGTCCCTTAACTAAAAAAAAGATTGTTTTGGTATTTTATTTTTATCTCCGTTACCTGTTTTGGTCCCTTCCGTTAAATAAATTCAAAAAAACGTTAGGGTTTGTACTATTCATCTTCTTCCTTCTCCTTCCATACTGTTCTTCATCATCAACAACACACCAACCCATATTTTGGGACCAAAACGATACCAAATAAATATTAAGGGATCAAAAGAACATTTAAGTTTTTTTTTTTTTTATCATCTTCTTCATCTTCATCAAAACTCATATTCATCAATTAAATCCAGAAAAAAAAAAAAAAAAAACACAGAATGCTCATATTTGGAAGAGTTATGCATAATGCTCAAATCAAAACTCATATTTGGCTTAAACTCATCAAAATAAAATAAAAAACACATAATGCTCAAATCAAAACTCAAATTTGGCTTAAACTCATATCAAAATCAAAACTTCAAAAGAGTTATGCATAATGCTCAAATTTGAAAAAACCTCCTTTAGAAAAACCCATCCTTCAAAAATTCGAAACAGGAGAAGAGTAAGGTTCTGCAGAATGAATTTGAAACAGGAAAAGAGTAAGATTATGCAGAATGAATTCGAAATGGGTGAAAATTAAGGTTCAAAAATTTGAGTTTTGCGAGAAGAGATTGAGGTTGTAAACAGGAGAAGATTTGAGTTTGAGATTCAACAAAGGATTACTTAAAGAAGGAGGTTTAAGTTTGAGATTCAACACAGGACACATAAGAGGAAAAGATTGAAGGATATGCTATTTTTTAGTTTTTTTTTGGATTATTTTCTGTGTTTTTTTTCTGGATTTAATTGATGAATATGAGTTTTGATGAAGATGAAGAAGAAAAACTTAAATGTTCTTTTGGTCCCTTAATTATTTATTTGGTATCGTTTTGGTCCCAAAATCTGGGTTTAGTTGATGAATGATGAAGGTCTGGGTTTAGTTGATGAATGATGAAGGTTTTGAGAAGGAAGAAGATGAATAGTACAAACCCTAACGTTTTTTTGAATTTATTTAACGGAAAGGACCAAAACAGGTAACAGAGATAAAGATAAAATACTAAAACAATCTTTTTTTTAGTTAAGGGACCAAAGCGATACCAAATAAATAGTTAAGGGACCAAAAGCGCATTTAAGCCTATTTTATAGTTGTACTCATACTATTGCAGGGCGAGGATACAGAATGGGTGACAGTAAAACTGGAGTCCCCAGAACCAACTAATGAAGACTGGGTTGGAGTTTTTACTCCTGCAAACTTCAAGTAACATACAACAATTCTCTCTCTCTCTCTCTCACTTTTAATATTGTTCTTTAAACACATTTTTCAAAAACATCATATCATACATATACTAAGACACTTGAGTGAATTTTGTATGAATCATATTGGTACATATTCACAATGTTAACACAGTCATGAAAAAGTACATTGACAATAGGAGTTTTTTGATTCCTCTTATGTTTTCTCTCTCTGTTTTATAATTGGAATAAAAAAAAAGAACTTGTAGTAAGTTTGCTCTCTAAAATTCAGCTCCATAACATTGCATGGTAGCCATGTGGAAAGATATGCCTTGGACTAACACAGAAGGAGTTAGTCACATGATCACACAACCTTATCTACACTTAGTCACATGAAACACCTTGGATGTAAGCAACATTCTGAATATGTCTAAAACAAAACCAACGTGAGTTAACTTACGGTGTTTGGAACGTTACTTAAGTTACGTAGTTTTTATTTCAATGTGATTTAAGTCATGTTGGGTTTAATATCTGCGCGACTTAAGTCACGTGGAGAGTATTTTAGGAAGTTTGATTGGGAGCAAGCAAATCTTGATGGGAGAAGAGCAATTTTCTTTATTTTACTTAATGACCCCTTAATTTTATGTTTGGGGTCCAAATTTTGAACTTATTTATTGGGACTGACGGTCGTCCTCCTCATCCTGCCTCAGATACGGCCCTTGTCATACCTATGTCTAGTAAACCTTATGTCATGTTCCTTAAATCCAATTTATCCAATGAGAGTTTCTTTTTTCACTCGCACGATTCTTAAGAACCGTTCGCATCTAAAAAAGGGATGGGAGGGGAGAGTCTTGAAGGAAAAAAAAATGAAGAATGAAGTGGATATAACAAAATATTTTGAAAGGGGAGGGTTTAGGAGTTTATTTTTCTTCATGGTACAAAATTCTTCCAACTTAAAACTTGTTTTTGTCGACAAATTCTAATTTAGAATTTAAAAACTTGTATTGAAGGATGATTTTGGAGGATTTTAGAAGATTAGGATAAATTTTCCAAATTTAATTTTTTTTTTTAAGGAAGGATAAATTTTCCAAATTTAATATATATTGTTATAATATTTTCAATATTAAAAATATATTAATCATAAGTATTTATTTATCATTTTCTACAAAACATTTCAAAAAAATAATCAATTCTCTAGTCTTTTCGCTACTCCCTCCTTCCCGAAGTTCTCCCATCCTTTTTCCTCCAAACTCTCCAACATCGCCTAAAGAGTCATGGCCATAAACTATGCATATATCTCACTATTATGGCTGAAAAACGAACTAAAGGAAATATATTATTGAAATCACAGTTCGTCAACATGTCCACCCAACCCCGGTGGCATTGGACGGGTAGATGCTCCATTCATATGCTCAGCCCCAATAAAGGTAAATGAAACTCCATCTCATATAAATTGTGTATGAAATGAATGAACAACATAACAAGGATGAACCTCTCATGTGTGTTGATTATAATTTGAATCTTTGTAATAGTACAAGTATGCCAACCACTCCAATCCTAAATACAAAAAGACTGGGAAAACTACTTTGAAGTTCCAATTGATCAATCAACGTGCAGATTTCTCCTTTGCACTATTTTCAGGTGGATTATCCAATGTAAGTCATATCTACTGATCTTTTTTTTTTTTTTTTACTATCTTTAAATTTTTTTGTCTTTGCTTAGATGTAGATTTCACATTTAAAAAATCAGTGTCATATACTAGTGCTATTAGAGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTGAAAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAACAGGGTAAGGCTGTGTACAATACACCAAATGGTGGGACCCCTTCCCAGACCCTGCATATGCGGGAGCTCTAGTGCACCGGGTTGCCCCTTTATATACTAGTGCTATTAGTATGCAATGATCCTTATTCAATTGTATGCTAACAAGTGTTCATTTGATTGAGCATTGCAGCCTAAGCTTGTTGCAGTTTCAAACTTCATTGCATTTGCAAATCCTAAAGCACCTGTGTATCCGCGCCTTGCTCATGGAAAGTCTTGGAATGAAGTAAGTTTATCTAGTCAATTTAATTGCATACTAGTTGCCTAACATTTCCTAATTATACATTGGTTCTTATCATCTAAAACTTTTGTAATTGAAATTGCTGCATGAATCGTCTAACTTAAACGTTTAGAATAATGATAAGAAATTTGTTCTTCTATGACAGATGACAGTTACCTGGACAAGTGGATATGACATAAGTGAAGCTGTACCATTTGTTGAATGGGGCCCAAAGGGTGGAAAGCAGATTCAATCTGCTGCTGGAACATTGACATTTAATCGTAACAGCATGTGTGGTATGGATTAATACATCGTAACTCAATTTCATACCTTATTATCTTTTTCTAAAAATGATTCTCAACATATAATGAGGGACATTTTGTTAATCTGTAGGTCAACCTGCGCGAACCGTTGGCTGGCGCGATCCTGGTTTTATACACACAGGTTTCCTTGAAGAACTTTGGCCAAATATGAGGTGTGCATTAAACTATATTTATGTTTATGAGACTATAATTGATAAGTAGAGTTCTGAAAGCTTAGCTACCTTCCCTCCCTATTCTTCCCCAGGTACGCCTATAGGTTGGGGCATTTATTGTCTGATGGCTCTTATGTATGGAGTAAGAGGTATTCATTCAAGGCATCTCCTTATCCTGGACAAAACTCATTGCAACGTGTTATCATATTCGGTGACATGGGAAAGGTAGCGTCGATTATTTAATGTTTATAACATTGGAAAGATTGATCACCTAACTAGATAATTTTCCAACTTTTTATCTAAAAACTCTAATTTGAAACTTGATTTGTACAATGGGGAGAACACTTATTTTGGTTCACATGTCCTTTAGGCTGAGCGTGATGGTTCAAATGAATATGCTAATTATCAACCTGGTTCACTTAACACCACTGACCAGCTCATCAAGGATTTAGACAATATTGACATTGTTTTCCACATAGGAGATCTGCCATATGCAAATGGATACATCTCACAGTGGGATCAATTCACAGCTCAAGTACAACAAATTTCATCAACAGTTCCATATATGATAGCAAGGTTCGCTTGCTTGCAATCATTTTAGGCCATAGAAATTTGAATTGAGTTTTACATATTCTTTTAGACTGAAAGGATATCACATTTATTCATACTGCAGTGGAAATCACGAACGCTCTTGGCCAAACTCAGGATCCTTCTATGACACTCCAGATTCAGGTGGAGAATGCGGAGTTCCTGCAGAAACCATGTACTATTACCCTGCTGAGAACAAAGCCAAATTCTGGTGAGTTATATTAATCTAAATGTAAACCATGACATGTTTAGCATTAATAAAACAGATTTTCTTTTCTCAACTGAATTTTTCACTACTTGGTCCAAGATAGTACGTATGATCCTGACCAATGGTTGCGTAATTATTTCTATAATTTCATTGTTGGAACAAAGTATGGTTTTGTGTTGATTTTTGAAAGAATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGATTGAGATTAAGAGATTAAGAGTTTGAGAATAATGAGAGGAGTAATATTGCTTTCTCAAATTCAGTTACAATATGCATACAACATAGAAGCTATTTATAGGCATGGTTTTGTTACAATAGATTGCTTAACACGCAAGCAATGCATATAACAAACCCTAACTGTTTTATTTGCAAGCTAAACAAACTTAAAACTAACTTCTAACAAACTTAACAAACTGAATTAACACATTCCAGCATTCATGATATCTGCAAAAGATACATTTTCATCCTTATAGTTCATGGAATCATGGTGCAGTTTCTAGTTTTTGCAAGTGAAAAATAAACTAGGAGGTACTATTTTTCCATTCCTAATTAATGACGAAAATTGGAAATATGCAGGTATGCCACAGATTATGGCATGTTTCGTTTCTGTATAGCAGACACTGAACATGACTGGAGAGAAGGATCGGAACAGTACAAATTCATCGAGCATTGCCTTGCAACAGTAGATAGAAAACAACAACCATGGTTAATCTTTGCAGCACATCGCCCACTCGGTTACTCCTCTAATGATTGGTATGCCAAGGAGGGTTCATTTGAAGAGCCCATGGGAAGGGAAAGTTTGCAGGGGCTTTGGCAAAAGTATAAAGTAGACATTGCATTTTATGGTCACGTCCATAACTACGAAAGAGTATGCCCCATTTATCAGGTATAACTACTATTGTCACATTTCAGTTAAAGCGAGATGTGCTGTCTATATTAAGTACTTGATTGTTACAGTTTTTACCGACAGCCTACATCCATTTCTAATTATCCAAATGTGTATAATGCAGAACCAATGCGTGAATAAAGAAAAATCTCATTATTCTGGCACTGTGAATGGAACAATTCATGTTGTTGTTGGTGGAGGGGGAAGTCACTTGTCAGACTTCACCACAGCACCTCCTGTTTGGAGTATTTTCAGAGACAAAGACTATGGTTTTGGCAAACTGACAGCATTTAATCACTCATATCTCTTGTTTGAGTACAAGAAAAGTAGTGATGGAAAGGTCTATGATTCTTTTACCATTTCCAGGGACTATAGAGATGTCTTGGCCTGTGTGCACGATGGTTGTGAGAAAACCACCTTGGCACTCTGA
Protein sequence

>MS.gene35568.t1

MEGKLNMSLVILCLFITNLRICLGDMNGFGEQPLSKIAIHKTILAIHDSASVTASPFLLGSKGEDTEWVTVKLESPEPTNEDWVGVFTPANFNSSTCPPNPGGIGRVDAPFICSAPIKYKYANHSNPKYKKTGKTTLKFQLINQRADFSFALFSGGLSNPKLVAVSNFIAFANPKAPVYPRLAHGKSWNEMTVTWTSGYDISEAVPFVEWGPKGGKQIQSAAGTLTFNRNSMCGQPARTVGWRDPGFIHTGFLEELWPNMRYAYRLGHLLSDGSYVWSKRYSFKASPYPGQNSLQRVIIFGDMGKAERDGSNEYANYQPGSLNTTDQLIKDLDNIDIVFHIGDLPYANGYISQWDQFTAQVQQISSTVPYMIASGNHERSWPNSGSFYDTPDSGGECGVPAETMYYYPAENKAKFWYATDYGMFRFCIADTEHDWREGSEQYKFIEHCLATVDRKQQPWLIFAAHRPLGYSSNDWYAKEGSFEEPMGRESLQGLWQKYKVDIAFYGHVHNYERVCPIYQNQCVNKEKSHYSGTVNGTIHVVVGGGGSHLSDFTTAPPVWSIFRDKDYGFGKLTAFNHSYLLFEYKKSSDGKVYDSFTISRDYRDVLACVHDGCEKTTLAL