Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36073.t1 | RHN40892.1 | 90 | 249 | 23 | 1 | 1 | 247 | 1 | 249 | 1.80E-95 | 359 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36073.t1 | A0A396GIE7 | 90.0 | 249 | 23 | 1 | 1 | 247 | 1 | 249 | 1.3e-95 | 359.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene36073 | 0.800859 | 1.18E-48 | -1.69E-46 |
| MS.gene049283 | MS.gene36073 | 0.814317 | 1.64E-51 | -1.69E-46 |
| MS.gene051597 | MS.gene36073 | 0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene36073 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene36073 | 0.809747 | 1.62E-50 | -1.69E-46 |
| MS.gene057893 | MS.gene36073 | -0.806323 | 8.70E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene36073 | -0.800003 | 1.77E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGAACAAAACATTTGATTT+AGG | 0.231644 | 8.1:-53026446 | MS.gene36073:CDS |
| TTTCTGTAGCCATACTTATT+AGG | 0.256262 | 8.1:+53026558 | None:intergenic |
| CTCATTCTGCTGAGAAACTA+TGG | 0.280675 | 8.1:+53026844 | None:intergenic |
| AAATATCAAAATCGTTATTC+AGG | 0.313436 | 8.1:+53026783 | None:intergenic |
| TATCTTCAAAGTCTGGGATA+TGG | 0.325886 | 8.1:-53026381 | MS.gene36073:CDS |
| CTGAGAGTATCTTCAAAGTC+TGG | 0.345707 | 8.1:-53026328 | MS.gene36073:CDS |
| TATCTTCAAAGTCTGGGATT+TGG | 0.348694 | 8.1:-53026321 | MS.gene36073:CDS |
| CTCTGTCAAAGAATTCTTTA+CGG | 0.352926 | 8.1:-53026253 | MS.gene36073:CDS |
| TCAACCCACGCAAGATCTTC+AGG | 0.354091 | 8.1:+53026812 | None:intergenic |
| TGCTGAGAAACTATGGAATT+AGG | 0.362253 | 8.1:+53026851 | None:intergenic |
| AGGAGAATATCTTCAAAGTC+TGG | 0.364718 | 8.1:-53026388 | MS.gene36073:CDS |
| TTGATATTTCAGAAAGTGAT+TGG | 0.365746 | 8.1:-53026769 | MS.gene36073:CDS |
| TAATGTTAGCCTAATAAGTA+TGG | 0.394006 | 8.1:-53026567 | MS.gene36073:CDS |
| TCGCTTGATGATCTTATTGC+TGG | 0.406967 | 8.1:-53026212 | MS.gene36073:CDS |
| TCTCAATCTTCTAAAGTTGA+TGG | 0.428199 | 8.1:-53026704 | MS.gene36073:CDS |
| ATCTTCAAAGTCTGGGATAT+GGG | 0.444852 | 8.1:-53026380 | MS.gene36073:CDS |
| AACTTTAGAAGATTGAGATT+CGG | 0.454226 | 8.1:+53026709 | None:intergenic |
| AGACAAGGTGTTAGTCAATT+CGG | 0.468773 | 8.1:+53026523 | None:intergenic |
| TCTAACAAAGCATCCCTCAT+AGG | 0.470432 | 8.1:+53026743 | None:intergenic |
| CTCAGGTCTTCATTATAAGT+TGG | 0.472539 | 8.1:+53026470 | None:intergenic |
| ACAAGTTCACCTTCCTCAGA+TGG | 0.481848 | 8.1:+53026293 | None:intergenic |
| TTCTCCTGAAGATCTTGCGT+GGG | 0.507872 | 8.1:-53026816 | MS.gene36073:CDS |
| AAAGTGATTGGATGCCTATG+AGG | 0.510324 | 8.1:-53026757 | MS.gene36073:CDS |
| TCTCATAAGTTGCAGAATCA+AGG | 0.515924 | 8.1:+53026417 | None:intergenic |
| GTCAAAGAATTCTTTACGGA+AGG | 0.516191 | 8.1:-53026249 | MS.gene36073:CDS |
| TTCTTTACGGAAGGACATGA+AGG | 0.523628 | 8.1:-53026240 | MS.gene36073:CDS |
| TCTAAAGTTGATGGTGGAGA+TGG | 0.528498 | 8.1:-53026695 | MS.gene36073:CDS |
| TGCAACTTATGAGATGGATC+AGG | 0.528709 | 8.1:-53026408 | MS.gene36073:CDS |
| TCGAAGATGACTGATTGAGT+TGG | 0.534472 | 8.1:+53026630 | None:intergenic |
| TGATTCTGCAACTTATGAGA+TGG | 0.544467 | 8.1:-53026414 | MS.gene36073:CDS |
| TGAGAGTATCTTCAAAGTCT+GGG | 0.548790 | 8.1:-53026327 | MS.gene36073:CDS |
| GGAGAATATCTTCAAAGTCT+GGG | 0.550962 | 8.1:-53026387 | MS.gene36073:CDS |
| GTATTTGTCTCCTCACTACA+AGG | 0.562243 | 8.1:+53026659 | None:intergenic |
| TTTCTCCTGAAGATCTTGCG+TGG | 0.566387 | 8.1:-53026817 | MS.gene36073:CDS |
| TGCAACTGATGAGAGTGAGC+AGG | 0.571996 | 8.1:-53026354 | MS.gene36073:CDS |
| GGATTTGGATATTCCATCTG+AGG | 0.589368 | 8.1:-53026306 | MS.gene36073:CDS |
| TTGGATATTCCATCTGAGGA+AGG | 0.595221 | 8.1:-53026302 | MS.gene36073:CDS |
| CAATCTTCTAAAGTTGATGG+TGG | 0.632930 | 8.1:-53026701 | MS.gene36073:CDS |
| TTGAAAAGTTGAAGAAGACA+AGG | 0.634159 | 8.1:+53026508 | None:intergenic |
| TCAAAGTATTCCTTGTAGTG+AGG | 0.650924 | 8.1:-53026669 | MS.gene36073:CDS |
| AAGTGATTGGATGCCTATGA+GGG | 0.653643 | 8.1:-53026756 | MS.gene36073:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATCAAAATCGTTATTC+AGG | + | chr8.1:53026297-53026316 | None:intergenic | 20.0% |
| ! | TTGATATTTCAGAAAGTGAT+TGG | - | chr8.1:53026308-53026327 | MS.gene36073:CDS | 25.0% |
| !! | AACTTTAGAAGATTGAGATT+CGG | + | chr8.1:53026371-53026390 | None:intergenic | 25.0% |
| !! | TAATGTTAGCCTAATAAGTA+TGG | - | chr8.1:53026510-53026529 | MS.gene36073:CDS | 25.0% |
| !! | TCATTATAAGTTGGCAAAAA+AGG | + | chr8.1:53026601-53026620 | None:intergenic | 25.0% |
| !!! | CCTAAATCAAATGTTTTGTT+CGG | + | chr8.1:53026634-53026653 | None:intergenic | 25.0% |
| CTCTGTCAAAGAATTCTTTA+CGG | - | chr8.1:53026824-53026843 | MS.gene36073:CDS | 30.0% | |
| TTGAAAAGTTGAAGAAGACA+AGG | + | chr8.1:53026572-53026591 | None:intergenic | 30.0% | |
| TTTCTGTAGCCATACTTATT+AGG | + | chr8.1:53026522-53026541 | None:intergenic | 30.0% | |
| ! | CCGAACAAAACATTTGATTT+AGG | - | chr8.1:53026631-53026650 | MS.gene36073:CDS | 30.0% |
| ! | TTGTCTTCTTCAACTTTTCA+AGG | - | chr8.1:53026571-53026590 | MS.gene36073:CDS | 30.0% |
| !! | TCTCAATCTTCTAAAGTTGA+TGG | - | chr8.1:53026373-53026392 | MS.gene36073:CDS | 30.0% |
| AGGAGAATATCTTCAAAGTC+TGG | - | chr8.1:53026689-53026708 | MS.gene36073:CDS | 35.0% | |
| CTCAGGTCTTCATTATAAGT+TGG | + | chr8.1:53026610-53026629 | None:intergenic | 35.0% | |
| GGAGAATATCTTCAAAGTCT+GGG | - | chr8.1:53026690-53026709 | MS.gene36073:CDS | 35.0% | |
| GTCAAAGAATTCTTTACGGA+AGG | - | chr8.1:53026828-53026847 | MS.gene36073:CDS | 35.0% | |
| TCTCATAAGTTGCAGAATCA+AGG | + | chr8.1:53026663-53026682 | None:intergenic | 35.0% | |
| TGATTCTGCAACTTATGAGA+TGG | - | chr8.1:53026663-53026682 | MS.gene36073:CDS | 35.0% | |
| TGCTGAGAAACTATGGAATT+AGG | + | chr8.1:53026229-53026248 | None:intergenic | 35.0% | |
| ! | AGACAAGGTGTTAGTCAATT+CGG | + | chr8.1:53026557-53026576 | None:intergenic | 35.0% |
| ! | ATCTTCAAAGTCTGGGATAT+GGG | - | chr8.1:53026697-53026716 | MS.gene36073:CDS | 35.0% |
| ! | TATCTTCAAAGTCTGGGATA+TGG | - | chr8.1:53026696-53026715 | MS.gene36073:CDS | 35.0% |
| ! | TATCTTCAAAGTCTGGGATT+TGG | - | chr8.1:53026756-53026775 | MS.gene36073:CDS | 35.0% |
| ! | TCAAAGTATTCCTTGTAGTG+AGG | - | chr8.1:53026408-53026427 | MS.gene36073:CDS | 35.0% |
| !! | CAATCTTCTAAAGTTGATGG+TGG | - | chr8.1:53026376-53026395 | MS.gene36073:CDS | 35.0% |
| !! | TGAGAGTATCTTCAAAGTCT+GGG | - | chr8.1:53026750-53026769 | MS.gene36073:CDS | 35.0% |
| AAAGTGATTGGATGCCTATG+AGG | - | chr8.1:53026320-53026339 | MS.gene36073:CDS | 40.0% | |
| AAGTGATTGGATGCCTATGA+GGG | - | chr8.1:53026321-53026340 | MS.gene36073:CDS | 40.0% | |
| CTCATTCTGCTGAGAAACTA+TGG | + | chr8.1:53026236-53026255 | None:intergenic | 40.0% | |
| GGATTTGGATATTCCATCTG+AGG | - | chr8.1:53026771-53026790 | MS.gene36073:CDS | 40.0% | |
| GTATTTGTCTCCTCACTACA+AGG | + | chr8.1:53026421-53026440 | None:intergenic | 40.0% | |
| TCGAAGATGACTGATTGAGT+TGG | + | chr8.1:53026450-53026469 | None:intergenic | 40.0% | |
| TGCAACTTATGAGATGGATC+AGG | - | chr8.1:53026669-53026688 | MS.gene36073:CDS | 40.0% | |
| TTCTTTACGGAAGGACATGA+AGG | - | chr8.1:53026837-53026856 | MS.gene36073:CDS | 40.0% | |
| TTGGATATTCCATCTGAGGA+AGG | - | chr8.1:53026775-53026794 | MS.gene36073:CDS | 40.0% | |
| ! | TCGCTTGATGATCTTATTGC+TGG | - | chr8.1:53026865-53026884 | MS.gene36073:CDS | 40.0% |
| ! | TCTAACAAAGCATCCCTCAT+AGG | + | chr8.1:53026337-53026356 | None:intergenic | 40.0% |
| !! | CAAATGTTTTGTTCGGTCTC+AGG | + | chr8.1:53026627-53026646 | None:intergenic | 40.0% |
| !! | CTGAGAGTATCTTCAAAGTC+TGG | - | chr8.1:53026749-53026768 | MS.gene36073:CDS | 40.0% |
| !! | TCTAAAGTTGATGGTGGAGA+TGG | - | chr8.1:53026382-53026401 | MS.gene36073:CDS | 40.0% |
| ACAAGTTCACCTTCCTCAGA+TGG | + | chr8.1:53026787-53026806 | None:intergenic | 45.0% | |
| TTCTCCTGAAGATCTTGCGT+GGG | - | chr8.1:53026261-53026280 | MS.gene36073:CDS | 45.0% | |
| TTTCTCCTGAAGATCTTGCG+TGG | - | chr8.1:53026260-53026279 | MS.gene36073:CDS | 45.0% | |
| TCAACCCACGCAAGATCTTC+AGG | + | chr8.1:53026268-53026287 | None:intergenic | 50.0% | |
| TGCAACTGATGAGAGTGAGC+AGG | - | chr8.1:53026723-53026742 | MS.gene36073:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 53026178 | 53026921 | 53026178 | ID=MS.gene36073 |
| chr8.1 | mRNA | 53026178 | 53026921 | 53026178 | ID=MS.gene36073.t1;Parent=MS.gene36073 |
| chr8.1 | exon | 53026178 | 53026921 | 53026178 | ID=MS.gene36073.t1.exon1;Parent=MS.gene36073.t1 |
| chr8.1 | CDS | 53026178 | 53026921 | 53026178 | ID=cds.MS.gene36073.t1;Parent=MS.gene36073.t1 |
| Gene Sequence |
| Protein sequence |
