AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36502

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36502.t1 MTR_3g450850 68.266 271 85 1 31 300 16 286 3.59e-129 385
MS.gene36502.t1 MTR_4g035820 38.462 390 196 4 60 447 74 421 2.57e-78 263
MS.gene36502.t1 MTR_4g071400 95.041 121 6 0 508 628 1 121 2.74e-74 234
MS.gene36502.t1 MTR_3g012160 81.522 92 17 0 210 301 2 93 3.34e-45 156
MS.gene36502.t1 MTR_1g070355 32.669 251 149 7 61 305 72 308 1.56e-28 121
MS.gene36502.t1 MTR_5g076070 27.917 240 157 7 64 300 56 282 8.00e-24 102
MS.gene36502.t1 MTR_3g095940 72.222 54 15 0 417 470 90 143 3.95e-19 85.1
MS.gene36502.t1 MTR_6g051425 25.000 264 183 6 62 319 18 272 8.90e-19 88.6
MS.gene36502.t1 MTR_6g014355 37.162 148 89 3 142 287 14 159 6.52e-17 81.3
MS.gene36502.t1 MTR_5g083630 54.286 70 26 1 204 267 7 76 1.62e-16 76.3
MS.gene36502.t1 MTR_6g022080 30.909 165 97 6 72 232 96 247 4.50e-14 73.2
MS.gene36502.t1 MTR_0558s0020 24.907 269 167 8 66 312 41 296 4.75e-14 73.9
MS.gene36502.t1 MTR_8g446020 26.531 196 124 5 126 312 72 256 1.25e-12 68.9
MS.gene36502.t1 MTR_1g019850 30.000 140 80 3 67 205 76 198 8.10e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36502.t1 AT5G28530 35.235 596 326 10 31 581 27 607 5.19e-116 361
MS.gene36502.t1 AT5G28530 35.067 596 331 10 31 581 27 611 2.16e-115 359
MS.gene36502.t1 AT5G28530 44.558 294 144 3 31 314 27 311 4.80e-75 254
MS.gene36502.t1 AT5G28530 37.023 262 158 2 321 581 415 670 1.34e-51 190
MS.gene36502.t1 AT5G28530 44.558 294 144 3 31 314 27 311 4.80e-75 254
MS.gene36502.t1 AT5G28530 37.023 262 158 2 321 581 415 670 1.34e-51 190
MS.gene36502.t1 AT5G28530 44.558 294 144 3 31 314 27 311 5.14e-75 254
MS.gene36502.t1 AT5G28530 36.641 262 163 2 321 581 415 674 6.54e-51 188
MS.gene36502.t1 AT1G10240 42.509 287 163 2 315 600 394 679 8.58e-73 248
MS.gene36502.t1 AT1G10240 46.715 274 134 5 48 315 30 297 5.21e-71 244
MS.gene36502.t1 AT1G10240 42.509 287 163 2 315 600 394 679 8.58e-73 248
MS.gene36502.t1 AT1G10240 46.715 274 134 5 48 315 30 297 5.21e-71 244
MS.gene36502.t1 AT4G15090 27.385 325 212 8 322 630 399 715 1.71e-27 119
MS.gene36502.t1 AT4G15090 26.154 260 172 8 61 315 51 295 1.32e-14 78.2
MS.gene36502.t1 AT4G15090 27.385 325 212 8 322 630 399 715 1.71e-27 119
MS.gene36502.t1 AT4G15090 26.154 260 172 8 61 315 51 295 1.32e-14 78.2
MS.gene36502.t1 AT4G15090 27.385 325 212 8 322 630 399 715 1.71e-27 119
MS.gene36502.t1 AT4G15090 26.154 260 172 8 61 315 51 295 1.32e-14 78.2
MS.gene36502.t1 AT4G15090 27.385 325 212 8 322 630 399 715 1.71e-27 119
MS.gene36502.t1 AT4G15090 26.154 260 172 8 61 315 51 295 1.32e-14 78.2
MS.gene36502.t1 AT4G15090 27.385 325 212 8 322 630 399 715 1.71e-27 119
MS.gene36502.t1 AT4G15090 26.154 260 172 8 61 315 51 295 1.32e-14 78.2
MS.gene36502.t1 AT4G38180 31.963 219 143 2 319 531 420 638 1.21e-26 116
MS.gene36502.t1 AT4G38180 27.953 254 171 4 61 312 73 316 4.03e-21 99.0
MS.gene36502.t1 AT4G38170 30.455 220 149 2 315 530 181 400 7.48e-26 112
MS.gene36502.t1 AT3G22170 27.778 270 175 8 322 582 408 666 1.42e-24 110
MS.gene36502.t1 AT3G22170 27.778 270 175 8 322 582 408 666 1.42e-24 110
MS.gene36502.t1 AT1G76320 27.014 211 153 1 315 524 329 539 6.56e-23 104
MS.gene36502.t1 AT1G76320 27.626 257 151 11 67 315 3 232 3.36e-12 70.1
MS.gene36502.t1 AT1G76320 27.014 211 153 1 315 524 329 539 6.77e-23 104
MS.gene36502.t1 AT1G76320 27.626 257 151 11 67 315 3 232 2.20e-12 70.9
MS.gene36502.t1 AT1G76320 27.014 211 153 1 315 524 329 539 6.79e-23 104
MS.gene36502.t1 AT1G76320 27.626 257 151 11 67 315 3 232 2.20e-12 70.9
MS.gene36502.t1 AT1G76320 27.014 211 153 1 315 524 329 539 6.79e-23 104
MS.gene36502.t1 AT1G76320 27.626 257 151 11 67 315 3 232 2.20e-12 70.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 289 501 3.43e-20 95.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 360 572 3.61e-20 95.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 346 558 3.69e-20 95.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 346 558 3.69e-20 95.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 346 558 3.79e-20 95.9
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 346 558 5.30e-20 95.5
MS.gene36502.t1 AT4G19990 26.636 214 150 3 321 528 346 558 5.30e-20 95.5
MS.gene36502.t1 AT5G18960 25.455 220 157 2 315 530 513 729 1.35e-19 94.0
MS.gene36502.t1 AT5G18960 25.455 220 157 2 315 530 513 729 1.35e-19 94.0
MS.gene36502.t1 AT2G27110 26.217 267 182 7 319 578 367 625 3.35e-19 92.8
MS.gene36502.t1 AT2G27110 26.217 267 182 7 319 578 367 625 3.35e-19 92.8
MS.gene36502.t1 AT2G27110 26.966 267 180 8 319 578 222 480 3.65e-19 92.4
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.17e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.35e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.35e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.48e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.48e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 345 611 1.48e-18 90.9
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 304 570 1.76e-18 90.5
MS.gene36502.t1 AT2G32250 24.453 274 197 3 324 594 304 570 1.96e-18 90.5
MS.gene36502.t1 AT1G52520 27.753 227 132 5 323 527 420 636 6.19e-18 88.6
MS.gene36502.t1 AT3G06250 23.636 220 161 2 315 530 489 705 8.40e-18 88.2
MS.gene36502.t1 AT3G06250 23.636 220 161 2 315 530 489 705 8.40e-18 88.2
MS.gene36502.t1 AT3G06250 23.636 220 161 2 315 530 489 705 8.40e-18 88.2
MS.gene36502.t1 AT1G80010 26.639 244 143 8 307 527 429 659 2.52e-14 77.0

Find 106 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTACATGACTAGATTT+TGG 0.131548 4.1:-18150254 None:intergenic
CCAATTAAAACTTACTTATT+AGG 0.177601 4.1:-18150653 None:intergenic
ATGAGATGCCTCTTGGAATA+TGG 0.199211 4.1:+18149432 MS.gene36502:CDS
TTCTCACAACCACGAGTTAT+TGG 0.199264 4.1:+18149010 MS.gene36502:CDS
ATCACATGTTCTTGAGTTTC+AGG 0.210618 4.1:-18150410 None:intergenic
CTCATGTTGAAGCAACTTAA+AGG 0.248230 4.1:-18149976 None:intergenic
TGTTTATTTGCAAAACAAAA+TGG 0.260407 4.1:+18148744 MS.gene36502:CDS
AAAATCTGTCAAACTTGTTT+TGG 0.261322 4.1:-18149739 None:intergenic
ATTCGTGCATATGAAGCTTT+TGG 0.265394 4.1:+18149368 MS.gene36502:CDS
CATCAATTGTGAATTCATAT+TGG 0.299021 4.1:-18149301 None:intergenic
AATATCATCATAAAGATAAA+AGG 0.310779 4.1:+18150383 MS.gene36502:CDS
TCAAGAACATGTGATTGATT+TGG 0.314506 4.1:+18150418 MS.gene36502:CDS
CAATATGAATTCACAATTGA+TGG 0.320293 4.1:+18149302 MS.gene36502:CDS
GCAGCTTCAACCCCTCCTTT+TGG 0.339248 4.1:-18150524 None:intergenic
ATTCAATCATATAGTGGTAT+TGG 0.341059 4.1:+18149212 MS.gene36502:CDS
ACTCTCGCTATAAGACTTAC+AGG 0.343612 4.1:-18149122 None:intergenic
CAAATTGGAGCATATTGTTT+GGG 0.347369 4.1:+18149331 MS.gene36502:CDS
GAGCATATTGTTTGGGCATT+TGG 0.348974 4.1:+18149338 MS.gene36502:CDS
ACAAATTGGAGCATATTGTT+TGG 0.352856 4.1:+18149330 MS.gene36502:CDS
AATTGATGGAAGTAACAAAT+TGG 0.358783 4.1:+18149316 MS.gene36502:CDS
ATGACAACGACGAGGCTTCT+TGG 0.366012 4.1:-18150546 None:intergenic
CCTCAAGTCACTAACATTGA+TGG 0.366209 4.1:+18148555 MS.gene36502:CDS
TCAGTCTCTAATTCGATGTT+TGG 0.372448 4.1:+18150304 MS.gene36502:CDS
AACGTCACAGTCAGCATTGT+TGG 0.377007 4.1:-18150453 None:intergenic
ATGGTCTCACAGTTTGGTTT+GGG 0.387072 4.1:+18149576 MS.gene36502:CDS
GTAGTTACTAAGAGAAGAAT+TGG 0.389295 4.1:+18148951 MS.gene36502:CDS
AATGGTCTCACAGTTTGGTT+TGG 0.398201 4.1:+18149575 MS.gene36502:CDS
TTCCAAGAGGCATCTCATAA+TGG 0.403997 4.1:-18149427 None:intergenic
TCAAATATTTGCCCTTCAAA+AGG 0.411166 4.1:-18148585 None:intergenic
ATCCATCGGGTGTTTATAAG+AGG 0.417116 4.1:+18148823 MS.gene36502:CDS
TTTGCTGGAATGACAACAAC+AGG 0.418779 4.1:+18149672 MS.gene36502:CDS
AAAGTAATGGTCTCACAGTT+TGG 0.427267 4.1:+18149570 MS.gene36502:CDS
TTGGGCCTTAGCTTATTTGA+AGG 0.435274 4.1:+18149641 MS.gene36502:CDS
TATACGACCACCATCAACTT+TGG 0.437731 4.1:-18150066 None:intergenic
GACGAGGCATGTTCTTCAAT+AGG 0.443666 4.1:-18149936 None:intergenic
AACCATTATGAGATGCCTCT+TGG 0.446043 4.1:+18149425 MS.gene36502:CDS
TGATTAGTTCCACCACAATC+TGG 0.446933 4.1:-18150572 None:intergenic
AAAGACAAGTGAACGAGAAC+AGG 0.453486 4.1:+18150340 MS.gene36502:CDS
CATGTAATAAACTGCAATGA+TGG 0.453622 4.1:+18150266 MS.gene36502:CDS
TGAGATGCCTCTTGGAATAT+GGG 0.456117 4.1:+18149433 MS.gene36502:CDS
GTTTGTCATCGTGGTGGTAT+TGG 0.458689 4.1:+18148852 MS.gene36502:CDS
ATGCGATCTTGATCAACAAT+TGG 0.465554 4.1:-18149074 None:intergenic
CTCACAGTTTGGTTTGGGTG+TGG 0.468983 4.1:+18149581 MS.gene36502:CDS
TATAAGACTTACAGGGCAAC+CGG 0.483233 4.1:-18149114 None:intergenic
ATAATGGTTTATTCTATATG+TGG 0.485058 4.1:-18149411 None:intergenic
CTTGAGTTAGAGAAAAGAAC+AGG 0.494786 4.1:+18149146 MS.gene36502:CDS
AAAACAAAAGGTCGGCCAAA+AGG 0.495104 4.1:+18150494 MS.gene36502:CDS
TTGATAGGATCATGAAGGGT+TGG 0.498564 4.1:+18150740 MS.gene36502:intron
TGAAGCAACTTAAAGGCATA+AGG 0.499495 4.1:-18149969 None:intergenic
CGTTGTCATGTTCCAGATTG+TGG 0.504732 4.1:+18150560 MS.gene36502:CDS
CCAAAAGGATCAAGACCAAA+AGG 0.514192 4.1:+18150509 MS.gene36502:CDS
CTTTCTCGTCTCCATCGAAA+AGG 0.515070 4.1:-18150221 None:intergenic
GTCAAAATTAGAAATCAAGC+TGG 0.517568 4.1:+18149858 MS.gene36502:CDS
ATTGGAATGTTACGATAAGC+AGG 0.522557 4.1:-18149056 None:intergenic
AATTGTGAATTCATATTGGA+GGG 0.524627 4.1:-18149297 None:intergenic
ATGTTATTGACTTGAGGCAA+GGG 0.525418 4.1:-18148657 None:intergenic
TAAGCAATGATGCTAGTGAT+TGG 0.525487 4.1:+18148526 MS.gene36502:CDS
ACCACGCAAAACTGTTGAAG+TGG 0.526679 4.1:+18148878 MS.gene36502:CDS
TTAGAGAAAAGAACAGGCAC+AGG 0.528687 4.1:+18149152 MS.gene36502:CDS
ATACTATTATTGTCTAAAGC+CGG 0.531055 4.1:+18149095 MS.gene36502:CDS
CAACATCATACCAAAGTTGA+TGG 0.538288 4.1:+18150056 MS.gene36502:CDS
AAGTCACTAACATTGATGGA+TGG 0.540573 4.1:+18148559 MS.gene36502:CDS
TGAACGAGAATGGAAAGTAA+TGG 0.544871 4.1:+18149557 MS.gene36502:CDS
CAATTGTGAATTCATATTGG+AGG 0.548419 4.1:-18149298 None:intergenic
TTGCACAACATAAGAGCCAT+TGG 0.551354 4.1:-18150036 None:intergenic
AGTCACTAACATTGATGGAT+GGG 0.552255 4.1:+18148560 MS.gene36502:CDS
TAAGAGAAGAATTGGCATTG+AGG 0.553953 4.1:+18148959 MS.gene36502:CDS
GTGCTTTGATAGGATCATGA+AGG 0.556862 4.1:+18150735 MS.gene36502:intron
CAAGTCAATAACATTGATGA+GGG 0.558520 4.1:+18148666 MS.gene36502:CDS
AAAGTCCTTCAAATAAGCTA+AGG 0.560983 4.1:-18149646 None:intergenic
ACATGTGATTGATTTGGAAG+AGG 0.565753 4.1:+18150424 MS.gene36502:CDS
GGAAAATGTGTTGTAATCTG+AGG 0.568974 4.1:-18149915 None:intergenic
ATGTGATTGTAGTGCAAAGA+TGG 0.570079 4.1:+18148929 MS.gene36502:CDS
ATGTTAGTGACTTGAGGCAA+GGG 0.572230 4.1:-18148549 None:intergenic
GTGTCTGAAGAGAATCCATC+GGG 0.572540 4.1:+18148810 MS.gene36502:CDS
TCATCAATGTTATTGACTTG+AGG 0.573525 4.1:-18148663 None:intergenic
TAGAAATCAAGCTGGAGAAG+AGG 0.577533 4.1:+18149866 MS.gene36502:CDS
CGAGTTATTGGATGACAAAG+AGG 0.580002 4.1:+18149022 MS.gene36502:CDS
AATGTTATTGACTTGAGGCA+AGG 0.580693 4.1:-18148658 None:intergenic
AATGTTAGTGACTTGAGGCA+AGG 0.597462 4.1:-18148550 None:intergenic
AGGGAATTTGTTTGTCATCG+TGG 0.598966 4.1:+18148843 MS.gene36502:CDS
TGTGTCTGAAGAGAATCCAT+CGG 0.599444 4.1:+18148809 MS.gene36502:CDS
TCCCTCTTATAAACACCCGA+TGG 0.599604 4.1:-18148825 None:intergenic
GCAGCAATTGAAGCTGCCAA+TGG 0.600590 4.1:+18150020 MS.gene36502:CDS
TCAAGTCAATAACATTGATG+AGG 0.603097 4.1:+18148665 MS.gene36502:CDS
CCATCAATGTTAGTGACTTG+AGG 0.606113 4.1:-18148555 None:intergenic
CTGTTGAAGTGGAAAATCAA+AGG 0.606328 4.1:+18148889 MS.gene36502:CDS
CTTTGTCATCCAATAACTCG+TGG 0.606716 4.1:-18149019 None:intergenic
AAGGATCAAGACCAAAAGGA+GGG 0.611165 4.1:+18150513 MS.gene36502:CDS
CTAAGGCCCAAAATTTACGA+TGG 0.615020 4.1:-18149629 None:intergenic
TAAGGCCCAAAATTTACGAT+GGG 0.615697 4.1:-18149628 None:intergenic
GAATTTGTTTGTCATCGTGG+TGG 0.618777 4.1:+18148846 MS.gene36502:CDS
TCCATCGGGTGTTTATAAGA+GGG 0.625908 4.1:+18148824 MS.gene36502:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 4.1:-18150641 None:intergenic
TGTCATGTTCCAGATTGTGG+TGG 0.637809 4.1:+18150563 MS.gene36502:CDS
CTCTCGCTATAAGACTTACA+GGG 0.647869 4.1:-18149121 None:intergenic
AAAGGATCAAGACCAAAAGG+AGG 0.655085 4.1:+18150512 MS.gene36502:CDS
TAGAGACTGAAATTTAGCAG+AGG 0.662901 4.1:-18150291 None:intergenic
ACACCAACCCATATTCCAAG+AGG 0.676376 4.1:-18149440 None:intergenic
TGCTTTGATAGGATCATGAA+GGG 0.676443 4.1:+18150736 MS.gene36502:intron
AAGTTTGAAAGATCGAGATG+AGG 0.682349 4.1:+18149274 MS.gene36502:CDS
CATCATACCAAAGTTGATGG+TGG 0.683172 4.1:+18150059 MS.gene36502:CDS
AGGATCAAGACCAAAAGGAG+GGG 0.696841 4.1:+18150514 MS.gene36502:CDS
AAGTCAATAACATTGATGAG+GGG 0.714581 4.1:+18148667 MS.gene36502:CDS
ATAAGGTGTGAGTATAGACG+AGG 0.720548 4.1:-18149952 None:intergenic
TCTGGAACATGACAACGACG+AGG 0.797744 4.1:-18150554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTAACTGTATTTTTA+TGG + chr4.1:18150713-18150732 MS.gene36502:intron 10.0%
!! AATATCATCATAAAGATAAA+AGG + chr4.1:18150383-18150402 MS.gene36502:CDS 15.0%
!!! AATTTTATTCAATCATATAG+TGG + chr4.1:18149206-18149225 MS.gene36502:CDS 15.0%
!! ATAATGGTTTATTCTATATG+TGG - chr4.1:18149414-18149433 None:intergenic 20.0%
!! CCAATTAAAACTTACTTATT+AGG - chr4.1:18150656-18150675 None:intergenic 20.0%
!! TGTTTATTTGCAAAACAAAA+TGG + chr4.1:18148744-18148763 MS.gene36502:CDS 20.0%
!!! AACAAAATGGATTTTCTATT+AGG + chr4.1:18148757-18148776 MS.gene36502:CDS 20.0%
!!! CATGGAAATTCAATTTTTTT+TGG + chr4.1:18149470-18149489 MS.gene36502:intron 20.0%
!!! CCTAATAAGTAAGTTTTAAT+TGG + chr4.1:18150653-18150672 MS.gene36502:intron 20.0%
!!! CTAATATCTTTTTCTAAGAA+TGG - chr4.1:18149185-18149204 None:intergenic 20.0%
!!! TGATTGTTATATTTTTGTGT+AGG + chr4.1:18149824-18149843 MS.gene36502:intron 20.0%
!!! TTATTACATGACTAGATTTT+GGG - chr4.1:18150256-18150275 None:intergenic 20.0%
!!! TTTATTACATGACTAGATTT+TGG - chr4.1:18150257-18150276 None:intergenic 20.0%
! AATTGATGGAAGTAACAAAT+TGG + chr4.1:18149316-18149335 MS.gene36502:CDS 25.0%
! AATTGTGAATTCATATTGGA+GGG - chr4.1:18149300-18149319 None:intergenic 25.0%
! ATTCAATCATATAGTGGTAT+TGG + chr4.1:18149212-18149231 MS.gene36502:CDS 25.0%
! CAATATGAATTCACAATTGA+TGG + chr4.1:18149302-18149321 MS.gene36502:CDS 25.0%
! CATCAATTGTGAATTCATAT+TGG - chr4.1:18149304-18149323 None:intergenic 25.0%
! TCCAAACAAAAAGAGAAAAA+TGG + chr4.1:18150610-18150629 MS.gene36502:CDS 25.0%
! TTGTTTCAAAAACAAAAGGT+CGG + chr4.1:18150486-18150505 MS.gene36502:CDS 25.0%
!! ATACTATTATTGTCTAAAGC+CGG + chr4.1:18149095-18149114 MS.gene36502:CDS 25.0%
!! GATATTTTCTAACTCTCTTT+TGG - chr4.1:18150369-18150388 None:intergenic 25.0%
!! TCAAATATTTTCCCTTCAAA+AGG - chr4.1:18148696-18148715 None:intergenic 25.0%
!! TGTAAAGAGTTTCAATTTTC+AGG + chr4.1:18150128-18150147 MS.gene36502:CDS 25.0%
!! TTTTACCCATCGTAAATTTT+GGG + chr4.1:18149623-18149642 MS.gene36502:CDS 25.0%
!! TTTTTACCCATCGTAAATTT+TGG + chr4.1:18149622-18149641 MS.gene36502:CDS 25.0%
!!! AAAATCTGTCAAACTTGTTT+TGG - chr4.1:18149742-18149761 None:intergenic 25.0%
!!! TATTTTTATGGTGCTTTGAT+AGG + chr4.1:18150725-18150744 MS.gene36502:intron 25.0%
!!! TCCATTTTTCTCTTTTTGTT+TGG - chr4.1:18150614-18150633 None:intergenic 25.0%
!!! TGTTATATTTTTGTGTAGGT+TGG + chr4.1:18149828-18149847 MS.gene36502:intron 25.0%
AAAGTCCTTCAAATAAGCTA+AGG - chr4.1:18149649-18149668 None:intergenic 30.0%
AAGTCAATAACATTGATGAG+GGG + chr4.1:18148667-18148686 MS.gene36502:CDS 30.0%
ACAAATTGGAGCATATTGTT+TGG + chr4.1:18149330-18149349 MS.gene36502:CDS 30.0%
CAAATTGGAGCATATTGTTT+GGG + chr4.1:18149331-18149350 MS.gene36502:CDS 30.0%
CAAGTCAATAACATTGATGA+GGG + chr4.1:18148666-18148685 MS.gene36502:CDS 30.0%
CAATTGTGAATTCATATTGG+AGG - chr4.1:18149301-18149320 None:intergenic 30.0%
CATGTAATAAACTGCAATGA+TGG + chr4.1:18150266-18150285 MS.gene36502:CDS 30.0%
GTAGTTACTAAGAGAAGAAT+TGG + chr4.1:18148951-18148970 MS.gene36502:CDS 30.0%
GTCAAAATTAGAAATCAAGC+TGG + chr4.1:18149858-18149877 MS.gene36502:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG - chr4.1:18150644-18150663 None:intergenic 30.0%
TCAAATATTTGCCCTTCAAA+AGG - chr4.1:18148588-18148607 None:intergenic 30.0%
TCAAGAACATGTGATTGATT+TGG + chr4.1:18150418-18150437 MS.gene36502:CDS 30.0%
TCAAGTCAATAACATTGATG+AGG + chr4.1:18148665-18148684 MS.gene36502:CDS 30.0%
! AATTGGCATTGAGGAAAAAT+GGG + chr4.1:18148968-18148987 MS.gene36502:CDS 30.0%
! CAAAATATCTTCCTTTTCGA+TGG + chr4.1:18150210-18150229 MS.gene36502:CDS 30.0%
!! CCTGTTGTTTCAAAAACAAA+AGG + chr4.1:18150482-18150501 MS.gene36502:CDS 30.0%
!! CGAAAAGGAAGATATTTTGA+TGG - chr4.1:18150209-18150228 None:intergenic 30.0%
!! TCATCAATGTTATTGACTTG+AGG - chr4.1:18148666-18148685 None:intergenic 30.0%
!!! CCTTTTGTTTTTGAAACAAC+AGG - chr4.1:18150485-18150504 None:intergenic 30.0%
!!! TTGAAGGACTTTTTTTTTGC+TGG + chr4.1:18149657-18149676 MS.gene36502:CDS 30.0%
AAAGTAATGGTCTCACAGTT+TGG + chr4.1:18149570-18149589 MS.gene36502:CDS 35.0%
AAGTCACTAACATTGATGGA+TGG + chr4.1:18148559-18148578 MS.gene36502:CDS 35.0%
AAGTTTGAAAGATCGAGATG+AGG + chr4.1:18149274-18149293 MS.gene36502:CDS 35.0%
ATCACATGTTCTTGAGTTTC+AGG - chr4.1:18150413-18150432 None:intergenic 35.0%
ATGCGATCTTGATCAACAAT+TGG - chr4.1:18149077-18149096 None:intergenic 35.0%
ATGTGATTGTAGTGCAAAGA+TGG + chr4.1:18148929-18148948 MS.gene36502:CDS 35.0%
ATTGGAATGTTACGATAAGC+AGG - chr4.1:18149059-18149078 None:intergenic 35.0%
CAACATCATACCAAAGTTGA+TGG + chr4.1:18150056-18150075 MS.gene36502:CDS 35.0%
CGAAATGAGAAGATTTCTTC+AGG + chr4.1:18149506-18149525 MS.gene36502:intron 35.0%
CTCATGTTGAAGCAACTTAA+AGG - chr4.1:18149979-18149998 None:intergenic 35.0%
CTGTTGAAGTGGAAAATCAA+AGG + chr4.1:18148889-18148908 MS.gene36502:CDS 35.0%
CTTGAGTTAGAGAAAAGAAC+AGG + chr4.1:18149146-18149165 MS.gene36502:CDS 35.0%
GGAAAATGTGTTGTAATCTG+AGG - chr4.1:18149918-18149937 None:intergenic 35.0%
TAAGAGAAGAATTGGCATTG+AGG + chr4.1:18148959-18148978 MS.gene36502:CDS 35.0%
TAAGCAATGATGCTAGTGAT+TGG + chr4.1:18148526-18148545 MS.gene36502:CDS 35.0%
TAAGGCCCAAAATTTACGAT+GGG - chr4.1:18149631-18149650 None:intergenic 35.0%
TAGAGACTGAAATTTAGCAG+AGG - chr4.1:18150294-18150313 None:intergenic 35.0%
TCAGTCTCTAATTCGATGTT+TGG + chr4.1:18150304-18150323 MS.gene36502:CDS 35.0%
TGAACGAGAATGGAAAGTAA+TGG + chr4.1:18149557-18149576 MS.gene36502:CDS 35.0%
TGCTTTGATAGGATCATGAA+GGG + chr4.1:18150736-18150755 MS.gene36502:intron 35.0%
! AGTCACTAACATTGATGGAT+GGG + chr4.1:18148560-18148579 MS.gene36502:CDS 35.0%
! ATTCGTGCATATGAAGCTTT+TGG + chr4.1:18149368-18149387 MS.gene36502:CDS 35.0%
! CAGATGATTTTGAACGAGAA+TGG + chr4.1:18149547-18149566 MS.gene36502:CDS 35.0%
! GAATTGGCATTGAGGAAAAA+TGG + chr4.1:18148967-18148986 MS.gene36502:CDS 35.0%
! TGAAGCAACTTAAAGGCATA+AGG - chr4.1:18149972-18149991 None:intergenic 35.0%
!! AATGTTATTGACTTGAGGCA+AGG - chr4.1:18148661-18148680 None:intergenic 35.0%
!! ACATGTGATTGATTTGGAAG+AGG + chr4.1:18150424-18150443 MS.gene36502:CDS 35.0%
!! ATGTTATTGACTTGAGGCAA+GGG - chr4.1:18148660-18148679 None:intergenic 35.0%
AAAACAAAAGGTCGGCCAAA+AGG + chr4.1:18150494-18150513 MS.gene36502:CDS 40.0%
AAAGACAAGTGAACGAGAAC+AGG + chr4.1:18150340-18150359 MS.gene36502:CDS 40.0%
AAAGGATCAAGACCAAAAGG+AGG + chr4.1:18150512-18150531 MS.gene36502:CDS 40.0%
AACCATTATGAGATGCCTCT+TGG + chr4.1:18149425-18149444 MS.gene36502:CDS 40.0%
AAGGATCAAGACCAAAAGGA+GGG + chr4.1:18150513-18150532 MS.gene36502:CDS 40.0%
AATGGTCTCACAGTTTGGTT+TGG + chr4.1:18149575-18149594 MS.gene36502:CDS 40.0%
AATGTTAGTGACTTGAGGCA+AGG - chr4.1:18148553-18148572 None:intergenic 40.0%
ACTCTCGCTATAAGACTTAC+AGG - chr4.1:18149125-18149144 None:intergenic 40.0%
ATAAGGTGTGAGTATAGACG+AGG - chr4.1:18149955-18149974 None:intergenic 40.0%
ATCCATCGGGTGTTTATAAG+AGG + chr4.1:18148823-18148842 MS.gene36502:CDS 40.0%
ATGAGATGCCTCTTGGAATA+TGG + chr4.1:18149432-18149451 MS.gene36502:CDS 40.0%
ATGTTAGTGACTTGAGGCAA+GGG - chr4.1:18148552-18148571 None:intergenic 40.0%
CCAAAAGGATCAAGACCAAA+AGG + chr4.1:18150509-18150528 MS.gene36502:CDS 40.0%
CCATCAATGTTAGTGACTTG+AGG - chr4.1:18148558-18148577 None:intergenic 40.0%
CCTCAAGTCACTAACATTGA+TGG + chr4.1:18148555-18148574 MS.gene36502:CDS 40.0%
CGAGTTATTGGATGACAAAG+AGG + chr4.1:18149022-18149041 MS.gene36502:CDS 40.0%
CTAAGGCCCAAAATTTACGA+TGG - chr4.1:18149632-18149651 None:intergenic 40.0%
CTCTCGCTATAAGACTTACA+GGG - chr4.1:18149124-18149143 None:intergenic 40.0%
CTTTGTCATCCAATAACTCG+TGG - chr4.1:18149022-18149041 None:intergenic 40.0%
GAGCATATTGTTTGGGCATT+TGG + chr4.1:18149338-18149357 MS.gene36502:CDS 40.0%
GTGCTTTGATAGGATCATGA+AGG + chr4.1:18150735-18150754 MS.gene36502:intron 40.0%
TAGAAATCAAGCTGGAGAAG+AGG + chr4.1:18149866-18149885 MS.gene36502:CDS 40.0%
TATAAGACTTACAGGGCAAC+CGG - chr4.1:18149117-18149136 None:intergenic 40.0%
TATACGACCACCATCAACTT+TGG - chr4.1:18150069-18150088 None:intergenic 40.0%
TCCATCGGGTGTTTATAAGA+GGG + chr4.1:18148824-18148843 MS.gene36502:CDS 40.0%
TGAGATGCCTCTTGGAATAT+GGG + chr4.1:18149433-18149452 MS.gene36502:CDS 40.0%
TGTGTCTGAAGAGAATCCAT+CGG + chr4.1:18148809-18148828 MS.gene36502:CDS 40.0%
TTAGAGAAAAGAACAGGCAC+AGG + chr4.1:18149152-18149171 MS.gene36502:CDS 40.0%
TTCTCACAACCACGAGTTAT+TGG + chr4.1:18149010-18149029 MS.gene36502:CDS 40.0%
TTGATAGGATCATGAAGGGT+TGG + chr4.1:18150740-18150759 MS.gene36502:intron 40.0%
TTGCACAACATAAGAGCCAT+TGG - chr4.1:18150039-18150058 None:intergenic 40.0%
TTGGGCCTTAGCTTATTTGA+AGG + chr4.1:18149641-18149660 MS.gene36502:CDS 40.0%
TTTGCTGGAATGACAACAAC+AGG + chr4.1:18149672-18149691 MS.gene36502:CDS 40.0%
! AGGGAATTTGTTTGTCATCG+TGG + chr4.1:18148843-18148862 MS.gene36502:CDS 40.0%
! ATGAGGGGATTCCTTTTGAA+GGG + chr4.1:18148682-18148701 MS.gene36502:CDS 40.0%
! ATGGTCTCACAGTTTGGTTT+GGG + chr4.1:18149576-18149595 MS.gene36502:CDS 40.0%
! GAATTTGTTTGTCATCGTGG+TGG + chr4.1:18148846-18148865 MS.gene36502:CDS 40.0%
! TGATTAGTTCCACCACAATC+TGG - chr4.1:18150575-18150594 None:intergenic 40.0%
!! ATGGATGGGTTCCTTTTGAA+GGG + chr4.1:18148574-18148593 MS.gene36502:CDS 40.0%
!! CATCATACCAAAGTTGATGG+TGG + chr4.1:18150059-18150078 MS.gene36502:CDS 40.0%
!! TGGGTTGGTGTTGATAATCA+TGG + chr4.1:18149452-18149471 MS.gene36502:intron 40.0%
!! TTCCAAGAGGCATCTCATAA+TGG - chr4.1:18149430-18149449 None:intergenic 40.0%
!!! CCTTTTGGTCTTGATCCTTT+TGG - chr4.1:18150512-18150531 None:intergenic 40.0%
AACGTCACAGTCAGCATTGT+TGG - chr4.1:18150456-18150475 None:intergenic 45.0%
ACACCAACCCATATTCCAAG+AGG - chr4.1:18149443-18149462 None:intergenic 45.0%
ACCACGCAAAACTGTTGAAG+TGG + chr4.1:18148878-18148897 MS.gene36502:CDS 45.0%
AGGATCAAGACCAAAAGGAG+GGG + chr4.1:18150514-18150533 MS.gene36502:CDS 45.0%
ATGCCTCTTGGAATATGGGT+TGG + chr4.1:18149437-18149456 MS.gene36502:intron 45.0%
CGTTGTCATGTTCCAGATTG+TGG + chr4.1:18150560-18150579 MS.gene36502:CDS 45.0%
CTTTCTCGTCTCCATCGAAA+AGG - chr4.1:18150224-18150243 None:intergenic 45.0%
GACGAGGCATGTTCTTCAAT+AGG - chr4.1:18149939-18149958 None:intergenic 45.0%
GTGTCTGAAGAGAATCCATC+GGG + chr4.1:18148810-18148829 MS.gene36502:CDS 45.0%
GTTTGTCATCGTGGTGGTAT+TGG + chr4.1:18148852-18148871 MS.gene36502:CDS 45.0%
TCCCTCTTATAAACACCCGA+TGG - chr4.1:18148828-18148847 None:intergenic 45.0%
TGTCATGTTCCAGATTGTGG+TGG + chr4.1:18150563-18150582 MS.gene36502:CDS 45.0%
! GATGAGGGGATTCCTTTTGA+AGG + chr4.1:18148681-18148700 MS.gene36502:CDS 45.0%
! TCCACTTCAACAGTTTTGCG+TGG - chr4.1:18148882-18148901 None:intergenic 45.0%
! TGGCATTGAGGAAAAATGGG+TGG + chr4.1:18148971-18148990 MS.gene36502:CDS 45.0%
!! GATGGATGGGTTCCTTTTGA+AGG + chr4.1:18148573-18148592 MS.gene36502:CDS 45.0%
ATGACAACGACGAGGCTTCT+TGG - chr4.1:18150549-18150568 None:intergenic 50.0%
GCAGCAATTGAAGCTGCCAA+TGG + chr4.1:18150020-18150039 MS.gene36502:CDS 50.0%
! TCTGGAACATGACAACGACG+AGG - chr4.1:18150557-18150576 None:intergenic 50.0%
!! CTCACAGTTTGGTTTGGGTG+TGG + chr4.1:18149581-18149600 MS.gene36502:CDS 50.0%
! GCAGCTTCAACCCCTCCTTT+TGG - chr4.1:18150527-18150546 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 18148513 18150768 18148513 ID=MS.gene36502
chr4.1 mRNA 18148513 18150768 18148513 ID=MS.gene36502.t1;Parent=MS.gene36502
chr4.1 exon 18148513 18149454 18148513 ID=MS.gene36502.t1.exon1;Parent=MS.gene36502.t1
chr4.1 CDS 18148513 18149454 18148513 ID=cds.MS.gene36502.t1;Parent=MS.gene36502.t1
chr4.1 exon 18149528 18149770 18149528 ID=MS.gene36502.t1.exon2;Parent=MS.gene36502.t1
chr4.1 CDS 18149528 18149770 18149528 ID=cds.MS.gene36502.t1;Parent=MS.gene36502.t1
chr4.1 exon 18149846 18150660 18149846 ID=MS.gene36502.t1.exon3;Parent=MS.gene36502.t1
chr4.1 CDS 18149846 18150660 18149846 ID=cds.MS.gene36502.t1;Parent=MS.gene36502.t1
chr4.1 exon 18150747 18150768 18150747 ID=MS.gene36502.t1.exon4;Parent=MS.gene36502.t1
chr4.1 CDS 18150747 18150768 18150747 ID=cds.MS.gene36502.t1;Parent=MS.gene36502.t1
Gene Sequence

>MS.gene36502

ATGATATCTGAAGTAAGCAATGATGCTAGTGATTGGCCCTTGCCTCAAGTCACTAACATTGATGGATGGGTTCCTTTTGAAGGGCAAATATTTGAAGATGATGAATATGTAAATTTTTTAACATTTCAAGAGCTACAACATGAACCCTTGCCTCAAGTCAATAACATTGATGAGGGGATTCCTTTTGAAGGGAAAATATTTGAAAGTGATAATGCTGCTTTTGAATTTTATTGTTTATTTGCAAAACAAAATGGATTTTCTATTAGGCGTGATCACATTTATAAATCTTGTAAATTTGTGTCTGAAGAGAATCCATCGGGTGTTTATAAGAGGGAATTTGTTTGTCATCGTGGTGGTATTGGTAAACCACGCAAAACTGTTGAAGTGGAAAATCAAAGGAAACGAAAATCTTCAAGATGTGATTGTAGTGCAAAGATGGTAGTTACTAAGAGAAGAATTGGCATTGAGGAAAAATGGGTGGTTAAACATTTCAACAATTCTCACAACCACGAGTTATTGGATGACAAAGAGGTTCAATTTCTTCCTGCTTATCGTAACATTCCAATTGTTGATCAAGATCGCATACTATTATTGTCTAAAGCCGGTTGCCCTGTAAGTCTTATAGCGAGAGTGCTTGAGTTAGAGAAAAGAACAGGCACAGGAAATTTACCATTCTTAGAAAAAGATATTAGAAATTTTATTCAATCATATAGTGGTATTGGTAAAGAAAGTGATGTTTCAGATGTTCTCAGATTGTGCAAAAGTTTGAAAGATCGAGATGAGGCCCTCCAATATGAATTCACAATTGATGGAAGTAACAAATTGGAGCATATTGTTTGGGCATTTGGTGATTCAATTCGTGCATATGAAGCTTTTGGAGATGTTGTAGTTTTTGATACCACATATAGAATAAACCATTATGAGATGCCTCTTGGAATATGGGTTGGTGTTGATAATCATGGAAATTCAATTTTTTTTGGTTGCGTTCTATTGCGAAATGAGAAGATTTCTTCAGGTGTATCATTTAGAATGTGCAGATGATTTTGAACGAGAATGGAAAGTAATGGTCTCACAGTTTGGTTTGGGTGTGGATAAGCATATTGACTTGCTTTTTACCCATCGTAAATTTTGGGCCTTAGCTTATTTGAAGGACTTTTTTTTTGCTGGAATGACAACAACAGGACGCTCAGAATCGATAAATTCATATATAAAAAGATTCTTAGATGCCAAAACAAGTTTGACAGATTTTGTAAATCAAGTAAGAACTTACTTGATACATTTTTTTTAATCAATTAGCTTATAATTTAGTCATGATTGTTATATTTTTGTGTAGGTTGGTGTTGCTGTCAAAATTAGAAATCAAGCTGGAGAAGAGGCACGAATGCGTCAAAAGTATCATAATCCTCAGATTACAACACATTTTCCTATTGAAGAACATGCCTCGTCTATACTCACACCTTATGCCTTTAAGTTGCTTCAACATGAGATCGAATTATCTGCAAAGTATGCAGCAATTGAAGCTGCCAATGGCTCTTATGTTGTGCAACATCATACCAAAGTTGATGGTGGTCGTATAGTAAGTTTGATAAAAGAAGAAGAGTCAATTCATTGTTCTTGTAAAGAGTTTCAATTTTCAGGAATAATATGTAGACATGCAATTCGAGTGCTAATGTTGAAAAATTATTTTGTTCTTCCATCAAAATATCTTCCTTTTCGATGGAGACGAGAAAGTTCATTGATCCCAAAATCTAGTCATGTAATAAACTGCAATGATGGATCCTCTGCTAAATTTCAGTCTCTAATTCGATGTTTGGAAGTCGAATCATTAAAGACAAGTGAACGAGAACAGGTTGCCAAAAGAGAGTTAGAAAATATCATCATAAAGATAAAAGGCATGCCTGAAACTCAAGAACATGTGATTGATTTGGAAGAGGATGTTTCCAACAATGCTGACTGTGACGTTGAAAATCCTGTTGTTTCAAAAACAAAAGGTCGGCCAAAAGGATCAAGACCAAAAGGAGGGGTTGAAGCTGCCAAGAAGCCTCGTCGTTGTCATGTTCCAGATTGTGGTGGAACTAATCACGACTCACGAAACTGTCCAAACAAAAAGAGAAAAATGGAAGTGTTGCCTTCTCAATCTCCTAATAAGTAAGTTTTAATTGGTGTGTCTTGCTCTACTCATGAGTAATATATTGAATTGTAATATTAACTGTATTTTTATGGTGCTTTGATAGGATCATGAAGGGTTGGAAGTAG
Protein sequence

>MS.gene36502.t1

MISEVSNDASDWPLPQVTNIDGWVPFEGQIFEDDEYVNFLTFQELQHEPLPQVNNIDEGIPFEGKIFESDNAAFEFYCLFAKQNGFSIRRDHIYKSCKFVSEENPSGVYKREFVCHRGGIGKPRKTVEVENQRKRKSSRCDCSAKMVVTKRRIGIEEKWVVKHFNNSHNHELLDDKEVQFLPAYRNIPIVDQDRILLLSKAGCPVSLIARVLELEKRTGTGNLPFLEKDIRNFIQSYSGIGKESDVSDVLRLCKSLKDRDEALQYEFTIDGSNKLEHIVWAFGDSIRAYEAFGDVVVFDTTYRINHYEMPLGIWVYHLECADDFEREWKVMVSQFGLGVDKHIDLLFTHRKFWALAYLKDFFFAGMTTTGRSESINSYIKRFLDAKTSLTDFVNQVGVAVKIRNQAGEEARMRQKYHNPQITTHFPIEEHASSILTPYAFKLLQHEIELSAKYAAIEAANGSYVVQHHTKVDGGRIVSLIKEEESIHCSCKEFQFSGIICRHAIRVLMLKNYFVLPSKYLPFRWRRESSLIPKSSHVINCNDGSSAKFQSLIRCLEVESLKTSEREQVAKRELENIIIKIKGMPETQEHVIDLEEDVSNNADCDVENPVVSKTKGRPKGSRPKGGVEAAKKPRRCHVPDCGGTNHDSRNCPNKKRKMEVLPSQSPNKIMKGWK