AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37227

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37227.t1 MTR_4g102520 98.116 584 7 2 1 580 1 584 0.0 1191
MS.gene37227.t1 MTR_1g072740 96.442 534 16 2 1 531 1 534 0.0 1070
MS.gene37227.t1 MTR_8g467040 33.226 310 193 4 278 577 107 412 1.63e-51 182
MS.gene37227.t1 MTR_4g119620 27.660 329 211 10 266 580 6 321 6.36e-33 129
MS.gene37227.t1 MTR_1g072140 25.163 306 216 8 279 580 16 312 3.79e-29 118
MS.gene37227.t1 MTR_7g086360 26.730 318 220 9 264 577 32 340 3.45e-26 110
MS.gene37227.t1 MTR_8g092620 27.687 307 203 10 278 580 6 297 9.50e-25 105
MS.gene37227.t1 MTR_8g092620 24.514 257 147 8 279 492 47 299 1.75e-12 68.9
MS.gene37227.t1 MTR_4g104380 25.962 312 206 8 278 580 218 513 2.15e-24 107
MS.gene37227.t1 MTR_3g075400 26.471 306 214 8 277 580 212 508 2.11e-23 104
MS.gene37227.t1 MTR_8g092620 27.541 305 202 10 278 578 13 302 2.98e-23 101
MS.gene37227.t1 MTR_2g436510 26.299 308 195 12 287 580 167 456 3.04e-19 91.3
MS.gene37227.t1 MTR_7g076230 26.132 287 188 9 288 556 367 647 6.00e-19 91.7
MS.gene37227.t1 MTR_8g092620 28.037 214 139 8 278 487 6 208 1.27e-16 79.7
MS.gene37227.t1 MTR_6g004040 29.825 171 111 6 281 449 92 255 2.28e-16 83.2
MS.gene37227.t1 MTR_6g004040 23.264 288 197 10 286 561 12 287 2.03e-14 77.0
MS.gene37227.t1 MTR_6g004040 24.868 189 135 4 392 580 31 212 6.99e-13 72.0
MS.gene37227.t1 MTR_6g004040 31.538 130 80 4 280 406 133 256 2.96e-11 67.0
MS.gene37227.t1 MTR_1g069345 24.691 324 227 11 263 580 191 503 6.06e-16 81.3
MS.gene37227.t1 MTR_2g012630 29.545 176 114 7 281 454 91 258 2.47e-15 80.1
MS.gene37227.t1 MTR_2g012630 23.737 198 144 4 383 580 21 211 7.66e-13 72.0
MS.gene37227.t1 MTR_2g012630 22.867 293 198 10 283 561 8 286 2.19e-12 70.5
MS.gene37227.t1 MTR_2g012630 29.545 176 114 7 281 454 91 258 2.73e-15 80.1
MS.gene37227.t1 MTR_2g012630 23.737 198 144 4 383 580 21 211 9.35e-13 72.0
MS.gene37227.t1 MTR_2g012630 22.867 293 198 10 283 561 8 286 2.65e-12 70.5
MS.gene37227.t1 MTR_1g097140 25.329 304 204 14 283 580 73 359 3.01e-15 78.6
MS.gene37227.t1 MTR_1g054300 23.097 381 203 10 281 580 110 481 4.19e-15 78.6
MS.gene37227.t1 MTR_5g090420 21.338 314 234 8 272 580 243 548 1.35e-14 77.0
MS.gene37227.t1 MTR_3g052220 23.824 319 217 11 279 580 202 511 2.64e-14 76.3
MS.gene37227.t1 MTR_3g116500 24.127 315 215 10 285 580 60 369 1.61e-13 72.8
MS.gene37227.t1 MTR_3g116500 26.222 225 150 7 369 580 38 259 7.20e-13 69.7
MS.gene37227.t1 MTR_8g106790 23.022 278 183 12 278 535 54 320 1.29e-12 69.7
MS.gene37227.t1 MTR_4g123597 23.125 320 211 14 281 580 347 651 2.18e-12 70.5
MS.gene37227.t1 MTR_4g105700 21.795 312 216 12 288 580 309 611 8.30e-12 68.6
MS.gene37227.t1 MTR_2g060520 21.429 322 228 8 279 580 115 431 1.04e-11 67.8
MS.gene37227.t1 MTR_1g034785 24.339 189 117 5 281 447 109 293 5.61e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37227.t1 AT1G10580 77.797 581 119 4 1 580 1 572 0.0 934
MS.gene37227.t1 AT5G54520 32.915 319 204 3 270 580 140 456 1.23e-54 192
MS.gene37227.t1 AT3G49660 25.641 312 214 9 277 580 11 312 5.93e-30 120
MS.gene37227.t1 AT3G49660 28.767 219 145 6 282 495 105 317 1.57e-25 107
MS.gene37227.t1 AT5G64730 27.508 309 201 11 278 580 6 297 5.77e-27 111
MS.gene37227.t1 AT4G15900 29.967 307 183 12 288 580 174 462 3.64e-26 112
MS.gene37227.t1 AT2G43770 26.460 291 206 7 287 574 50 335 9.50e-26 108
MS.gene37227.t1 AT4G02730 24.595 309 215 10 279 580 32 329 4.09e-24 103
MS.gene37227.t1 AT3G16650 28.990 307 185 13 288 580 168 455 5.71e-22 99.8
MS.gene37227.t1 AT5G50230 24.658 292 196 7 278 560 213 489 6.64e-20 93.6
MS.gene37227.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.49e-17 86.7
MS.gene37227.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.49e-17 86.7
MS.gene37227.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.49e-17 86.7
MS.gene37227.t1 AT5G23430 23.256 301 200 12 283 574 9 287 2.59e-17 86.3
MS.gene37227.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.86e-14 77.4
MS.gene37227.t1 AT5G23430 25.000 192 131 4 392 580 31 212 1.10e-12 71.6
MS.gene37227.t1 AT5G23430 29.921 127 86 3 280 406 133 256 4.32e-11 66.6
MS.gene37227.t1 AT5G23430 23.256 301 200 12 283 574 9 287 2.72e-17 86.3
MS.gene37227.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.98e-14 77.0
MS.gene37227.t1 AT5G23430 25.000 192 131 4 392 580 31 212 1.16e-12 71.6
MS.gene37227.t1 AT5G23430 29.921 127 86 3 280 406 133 256 4.36e-11 66.6
MS.gene37227.t1 AT5G23430 23.256 301 200 12 283 574 9 287 2.72e-17 86.3
MS.gene37227.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.98e-14 77.0
MS.gene37227.t1 AT5G23430 25.000 192 131 4 392 580 31 212 1.16e-12 71.6
MS.gene37227.t1 AT5G23430 29.921 127 86 3 280 406 133 256 4.36e-11 66.6
MS.gene37227.t1 AT5G08390 23.490 298 203 11 283 574 9 287 4.45e-17 85.5
MS.gene37227.t1 AT5G08390 32.000 150 94 5 281 428 92 235 2.45e-15 80.1
MS.gene37227.t1 AT5G08390 25.000 192 131 4 392 580 31 212 6.96e-13 72.0
MS.gene37227.t1 AT5G08390 32.283 127 83 3 280 406 133 256 2.55e-12 70.5
MS.gene37227.t1 AT1G61210 31.429 175 112 6 281 454 91 258 9.77e-17 84.7
MS.gene37227.t1 AT1G61210 25.714 280 182 10 288 563 13 270 1.08e-15 81.3
MS.gene37227.t1 AT1G61210 31.008 129 82 5 280 406 132 255 1.80e-11 67.8
MS.gene37227.t1 AT1G61210 23.810 189 137 4 392 580 30 211 2.86e-11 67.0
MS.gene37227.t1 AT1G61210 31.429 175 112 6 281 454 91 258 1.24e-16 84.3
MS.gene37227.t1 AT1G61210 25.714 280 182 10 288 563 13 270 1.55e-15 80.9
MS.gene37227.t1 AT1G61210 31.008 129 82 5 280 406 132 255 2.08e-11 67.8
MS.gene37227.t1 AT1G61210 23.810 189 137 4 392 580 30 211 3.18e-11 67.0
MS.gene37227.t1 AT1G61210 31.429 175 112 6 281 454 91 258 1.28e-16 84.3
MS.gene37227.t1 AT1G61210 25.714 280 182 10 288 563 13 270 1.74e-15 80.9
MS.gene37227.t1 AT1G61210 31.008 129 82 5 280 406 132 255 2.16e-11 67.8
MS.gene37227.t1 AT1G61210 23.810 189 137 4 392 580 30 211 3.07e-11 67.0
MS.gene37227.t1 AT1G15440 23.203 306 215 7 288 577 347 648 1.52e-16 84.0
MS.gene37227.t1 AT1G15440 26.457 223 156 5 288 506 387 605 1.95e-16 83.6
MS.gene37227.t1 AT5G43920 24.688 320 218 12 274 580 204 513 2.17e-16 82.8
MS.gene37227.t1 AT1G11160 24.742 291 195 9 283 561 8 286 7.58e-16 81.6
MS.gene37227.t1 AT1G11160 27.684 177 120 6 281 456 91 260 8.87e-15 78.6
MS.gene37227.t1 AT1G11160 25.397 189 134 4 392 580 30 211 2.36e-14 77.0
MS.gene37227.t1 AT1G11160 30.534 131 80 6 280 406 132 255 7.43e-11 65.9
MS.gene37227.t1 AT1G11160 24.742 291 195 9 283 561 8 286 7.58e-16 81.6
MS.gene37227.t1 AT1G11160 27.684 177 120 6 281 456 91 260 8.65e-15 78.6
MS.gene37227.t1 AT1G11160 25.397 189 134 4 392 580 30 211 2.20e-14 77.0
MS.gene37227.t1 AT1G11160 30.534 131 80 6 280 406 132 255 7.43e-11 65.9
MS.gene37227.t1 AT1G11160 24.742 291 195 9 283 561 8 286 8.12e-16 81.6
MS.gene37227.t1 AT1G11160 27.684 177 120 6 281 456 91 260 8.87e-15 78.6
MS.gene37227.t1 AT1G11160 25.397 189 134 4 392 580 30 211 2.44e-14 77.0
MS.gene37227.t1 AT1G11160 30.534 131 80 6 280 406 132 255 7.51e-11 65.9
MS.gene37227.t1 AT1G11160 27.684 177 120 6 281 456 49 218 7.91e-15 78.6
MS.gene37227.t1 AT1G11160 25.926 162 113 4 419 580 15 169 2.75e-12 70.5
MS.gene37227.t1 AT1G11160 24.803 254 168 8 320 561 2 244 1.94e-11 67.8
MS.gene37227.t1 AT1G11160 30.534 131 80 6 280 406 90 213 7.38e-11 65.9
MS.gene37227.t1 AT3G18130 24.907 269 175 11 287 535 60 321 5.53e-14 73.9
MS.gene37227.t1 AT3G18130 24.907 269 184 9 289 549 14 272 1.06e-11 67.0
MS.gene37227.t1 AT5G52820 24.497 298 204 10 287 580 191 471 3.37e-13 72.4
MS.gene37227.t1 AT1G48630 24.907 269 177 11 287 537 60 321 5.74e-13 70.9
MS.gene37227.t1 AT1G48630 24.542 273 188 9 285 549 10 272 5.80e-12 67.8
MS.gene37227.t1 AT2G33340 25.591 254 173 9 281 528 255 498 7.01e-13 71.6
MS.gene37227.t1 AT2G33340 25.591 254 173 9 281 528 255 498 7.01e-13 71.6
MS.gene37227.t1 AT5G16750 25.444 169 118 5 285 448 55 220 8.60e-13 72.0
MS.gene37227.t1 AT2G41500 23.228 254 179 7 331 580 252 493 1.10e-12 71.2
MS.gene37227.t1 AT2G41500 25.472 212 152 6 282 492 331 537 4.65e-12 69.3
MS.gene37227.t1 AT2G41500 23.228 254 179 7 331 580 252 493 1.10e-12 71.2
MS.gene37227.t1 AT2G41500 25.472 212 152 6 282 492 331 537 4.65e-12 69.3
MS.gene37227.t1 AT1G73720 24.451 319 214 12 279 580 201 509 3.56e-12 69.3
MS.gene37227.t1 AT3G21540 23.864 264 174 12 330 580 402 651 7.27e-12 68.9
MS.gene37227.t1 AT3G21540 27.094 203 134 8 299 494 26 221 5.75e-11 66.2
MS.gene37227.t1 AT2G33340 27.011 174 117 6 281 449 255 423 1.51e-11 67.4
MS.gene37227.t1 AT1G79990 22.460 187 141 3 306 490 72 256 1.74e-11 67.8
MS.gene37227.t1 AT1G18080 23.420 269 182 9 287 537 60 322 1.82e-11 66.2
MS.gene37227.t1 AT1G18080 22.695 282 161 7 285 558 10 242 1.85e-11 66.2
MS.gene37227.t1 AT1G79990 22.460 187 141 3 306 490 72 256 2.06e-11 67.4
MS.gene37227.t1 AT5G60940 22.812 320 225 9 278 580 111 425 9.20e-11 64.7

Find 164 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTCTTCTTTCTTCTTA+AGG 0.216922 8.3:-19218607 None:intergenic
GTGAGGGTAAGTGTTGGTTC+TGG 0.241552 8.3:+19219707 MS.gene37227:CDS
AGCATGGTGTCGTCGACTTT+GGG 0.258974 8.3:-19218227 None:intergenic
ACCCTACCTATGACCAACTA+TGG 0.277536 8.3:+19218321 MS.gene37227:CDS
ATTCTCTTCTTTCTTCTTAA+GGG 0.283276 8.3:-19218606 None:intergenic
CGCTTCACCCCAACGCGAAT+TGG 0.301825 8.3:+19219527 MS.gene37227:CDS
CAAGAGTATGATCAGCATTT+GGG 0.309524 8.3:+19219358 MS.gene37227:CDS
TGGATACTAAGGTTAAGATT+TGG 0.323671 8.3:+19219050 MS.gene37227:CDS
GGATACTAAGGTTAAGATTT+GGG 0.331103 8.3:+19219051 MS.gene37227:CDS
TGATAAGAATATTAAGTATT+GGG 0.340716 8.3:+19219180 MS.gene37227:CDS
GCGGCGAGCCAATTCGCGTT+GGG 0.343990 8.3:-19219535 None:intergenic
CGATATAACCTGCCCTGTTT+CGG 0.352782 8.3:-19219206 None:intergenic
TGAGGGTAAGTGTTGGTTCT+GGG 0.355149 8.3:+19219708 MS.gene37227:CDS
TGGGTCATTCCAAAGCTGTT+AGG 0.358107 8.3:+19219110 MS.gene37227:CDS
TCAAGAGTATGATCAGCATT+TGG 0.358810 8.3:+19219357 MS.gene37227:CDS
TAGCATGGTGTCGTCGACTT+TGG 0.362731 8.3:-19218228 None:intergenic
TCCCTCAGGGTATGGGAGTA+TGG 0.364907 8.3:+19219445 MS.gene37227:CDS
CAAAGAGATTGGTGCATACT+TGG 0.367144 8.3:+19218954 MS.gene37227:CDS
ACATTCTGTTTATCCTCATC+CGG 0.374087 8.3:-19219271 None:intergenic
GATGGGTTTAGAGAGGGTTT+TGG 0.379523 8.3:-19218270 None:intergenic
AAGTATTGGGATACCGAAAC+AGG 0.381659 8.3:+19219193 MS.gene37227:CDS
CAAAAGGGGTTTCTGCGATT+AGG 0.383880 8.3:+19218987 MS.gene37227:CDS
CAAGTATGCACCAATCTCTT+TGG 0.386596 8.3:-19218953 None:intergenic
TTTGCTGGACACATTGTTGC+TGG 0.388967 8.3:+19219628 MS.gene37227:CDS
ACAAATATCCCTAACAGCTT+TGG 0.389574 8.3:-19219119 None:intergenic
GGGTTAAGCTTCACAACATA+AGG 0.392453 8.3:-19219250 None:intergenic
CTCTTCTTCTGTTCGTGGTG+TGG 0.395282 8.3:-19218572 None:intergenic
AGAATCAAATGACCCGAATT+CGG 0.398493 8.3:-19219016 None:intergenic
CGTAAGGGTGAGACGGGCCT+TGG 0.399519 8.3:-19218352 None:intergenic
TTACTCGCCTTAGCATCTTT+CGG 0.401503 8.3:-19218914 None:intergenic
GTAAGTGTTGGTTCTGGGAT+TGG 0.407813 8.3:+19219713 MS.gene37227:CDS
CGCGTTGGGGTGAAGCGAAA+TGG 0.409944 8.3:-19219521 None:intergenic
GCAAAGTAGCGACGTGTGGC+TGG 0.411956 8.3:+19219815 MS.gene37227:CDS
ATGATAAGAATATTAAGTAT+TGG 0.420715 8.3:+19219179 MS.gene37227:CDS
TTGTGCTATAAATAAGAATC+TGG 0.429963 8.3:-19219573 None:intergenic
GGGATTATCAAGGTAGGTCT+TGG 0.431742 8.3:+19218882 MS.gene37227:CDS
CGCGGCGAGCCAATTCGCGT+TGG 0.432970 8.3:-19219536 None:intergenic
TCTTCTTCTGTTCGTGGTGT+GGG 0.434059 8.3:-19218571 None:intergenic
ATGACAAGTCCCTCAGGGTA+TGG 0.434880 8.3:+19219437 MS.gene37227:CDS
GAGTCCTTGGGCTGGGAAGA+AGG 0.437077 8.3:+19218715 MS.gene37227:CDS
AATAACCACGCCTCAGAAGC+TGG 0.437350 8.3:-19218680 None:intergenic
TTGGCTCGCCGCGCAGAGCT+TGG 0.439153 8.3:+19219546 MS.gene37227:CDS
CGGTTGCCAGTGGCATCCTC+TGG 0.441347 8.3:+19219786 MS.gene37227:CDS
TGAAGAATAAGAAGAGTCCT+TGG 0.443248 8.3:+19218702 MS.gene37227:CDS
GGGCGGCAGAACGGGATGGG+AGG 0.448266 8.3:-19218205 None:intergenic
CTGGATCGTTGTCGGAGTAT+TGG 0.449647 8.3:-19218133 None:intergenic
AGAACGGGATGGGAGGAGAC+GGG 0.450151 8.3:-19218198 None:intergenic
CAATACTCCGACAACGATCC+AGG 0.450178 8.3:+19218134 MS.gene37227:CDS
GTTCAGTGGGATATGAATAC+AGG 0.451594 8.3:+19219325 MS.gene37227:CDS
GGGTCATTCCAAAGCTGTTA+GGG 0.454301 8.3:+19219111 MS.gene37227:CDS
GAACGGGATGGGAGGAGACG+GGG 0.455804 8.3:-19218197 None:intergenic
AGGTTTACAAGGAGAGTTAA+CGG 0.457451 8.3:+19218739 MS.gene37227:CDS
TTTGGGGTCGGCTACAGTTA+GGG 0.461852 8.3:-19218252 None:intergenic
GTTATATCGACGTTCTCCAC+TGG 0.463024 8.3:+19219220 MS.gene37227:CDS
TTCGGGTCATTTGATTCTCT+CGG 0.463515 8.3:+19219021 MS.gene37227:CDS
CGGGATGGGAGGAGACGGGG+TGG 0.467337 8.3:-19218194 None:intergenic
GTTTGTGGTTACGCATACCT+TGG 0.469238 8.3:-19218391 None:intergenic
TAGCGACGTGTGGCTGGGAC+GGG 0.469608 8.3:+19219821 MS.gene37227:CDS
GAGGAGCACGCCAAGAAGAA+AGG 0.472310 8.3:+19218782 MS.gene37227:CDS
CTAGTGATGACAAGTCCCTC+AGG 0.475381 8.3:+19219431 MS.gene37227:CDS
TCAACACTGGTAAATGTATG+AGG 0.476160 8.3:+19219080 MS.gene37227:CDS
ACTTGGAGCGGACATACAAA+AGG 0.478707 8.3:+19218971 MS.gene37227:CDS
GGGAGCCCATAGTTGGTCAT+AGG 0.481488 8.3:-19218327 None:intergenic
CTTGGATGGGAGCCCATAGT+TGG 0.481531 8.3:-19218334 None:intergenic
CAGAACGGGATGGGAGGAGA+CGG 0.487345 8.3:-19218199 None:intergenic
AGGCGACAAGGTGTTGAGTT+GGG 0.487361 8.3:-19218295 None:intergenic
AACTCAAAACCCTGATTCAC+CGG 0.489991 8.3:+19218163 MS.gene37227:CDS
CTTCGACGAAACCAAGTTTG+TGG 0.490052 8.3:-19218406 None:intergenic
CTCGGCTGGTATGGATACTA+AGG 0.491505 8.3:+19219039 MS.gene37227:CDS
TTGGGTCGATGGGTTTAGAG+AGG 0.491971 8.3:-19218277 None:intergenic
TTTGATTCTCTCGGCTGGTA+TGG 0.492749 8.3:+19219030 MS.gene37227:CDS
AGGGTGAGACGGGCCTTGGA+TGG 0.498298 8.3:-19218348 None:intergenic
GGTCATTTGATTCTCTCGGC+TGG 0.501830 8.3:+19219025 MS.gene37227:CDS
TTTGTGGTTACGCATACCTT+GGG 0.502829 8.3:-19218390 None:intergenic
TGTTGCTGGATATGCTTGTC+AGG 0.503005 8.3:+19219642 MS.gene37227:CDS
AGGTGTTGAGTTGGGTCGAT+GGG 0.504885 8.3:-19218287 None:intergenic
GGGTGAGACGGGCCTTGGAT+GGG 0.513343 8.3:-19218347 None:intergenic
GCTAAACCGTCTTTAGCGTA+AGG 0.515618 8.3:-19218368 None:intergenic
TGGTGAACCTGGATCGTTGT+CGG 0.515766 8.3:-19218141 None:intergenic
CAAAGTAGCGACGTGTGGCT+GGG 0.520964 8.3:+19219816 MS.gene37227:CDS
CATTCTGTTTATCCTCATCC+GGG 0.523411 8.3:-19219270 None:intergenic
GTAGCGACGTGTGGCTGGGA+CGG 0.525013 8.3:+19219820 MS.gene37227:CDS
TTGGTCATAGGTAGGGTTGA+AGG 0.527966 8.3:-19218315 None:intergenic
CGCGCCTCCGAAAGATGCTA+AGG 0.529557 8.3:+19218907 MS.gene37227:CDS
CTAATATACTTTATAACCAC+GGG 0.530865 8.3:-19219472 None:intergenic
CTTGGAGCGGACATACAAAA+GGG 0.531466 8.3:+19218972 MS.gene37227:CDS
AAGGCGACAAGGTGTTGAGT+TGG 0.535225 8.3:-19218296 None:intergenic
TGTCATGTCGGGAGATGGTG+AGG 0.538558 8.3:+19219690 MS.gene37227:CDS
TGATAAGAAGATTGTTCAGT+GGG 0.538924 8.3:+19219312 MS.gene37227:CDS
ATAAGAAGAGTCCTTGGGCT+GGG 0.539663 8.3:+19218708 MS.gene37227:CDS
GCCCATAGTTGGTCATAGGT+AGG 0.539722 8.3:-19218323 None:intergenic
GATTGAGAATCCAGCTTCTG+AGG 0.541239 8.3:+19218670 MS.gene37227:CDS
TTATGTTGTGAAGCTTAACC+CGG 0.541534 8.3:+19219252 MS.gene37227:CDS
AAGGTGTTGAGTTGGGTCGA+TGG 0.542762 8.3:-19218288 None:intergenic
TGGGTCGATGGGTTTAGAGA+GGG 0.542777 8.3:-19218276 None:intergenic
GGTATGCGTAACCACAAACT+TGG 0.542950 8.3:+19218395 MS.gene37227:CDS
GCATGGTGTCGTCGACTTTG+GGG 0.545386 8.3:-19218226 None:intergenic
GAAGAATAAGAAGAGTCCTT+GGG 0.545960 8.3:+19218703 MS.gene37227:CDS
CAACTATGGGCTCCCATCCA+AGG 0.546220 8.3:+19218335 MS.gene37227:CDS
GTTGTTATCAACAAAAGTAA+TGG 0.548421 8.3:-19219392 None:intergenic
CTTTAGCGTAAGGGTGAGAC+GGG 0.549707 8.3:-19218358 None:intergenic
TGACAAGTCCCTCAGGGTAT+GGG 0.550285 8.3:+19219438 MS.gene37227:CDS
TTATATCGACGTTCTCCACT+GGG 0.551618 8.3:+19219221 MS.gene37227:CDS
CACACCACGAACAGAAGAAG+AGG 0.552504 8.3:+19218573 MS.gene37227:CDS
CCTTCCTTCTTCCCAGCCCA+AGG 0.553576 8.3:-19218719 None:intergenic
TGCTGTTCATCAAAGAGGAA+AGG 0.560443 8.3:-19218443 None:intergenic
GGGACGGGTTGATAAAGTAC+TGG 0.561357 8.3:+19219836 MS.gene37227:CDS
GGTTAAGCTTCACAACATAA+GGG 0.563836 8.3:-19219249 None:intergenic
CCTTGGGCTGGGAAGAAGGA+AGG 0.564772 8.3:+19218719 MS.gene37227:CDS
CGGCGAGCCAATTCGCGTTG+GGG 0.566063 8.3:-19219534 None:intergenic
GGGAAGAAGGAAGGTTTACA+AGG 0.566983 8.3:+19218728 MS.gene37227:CDS
AATAAGAAGAGTCCTTGGGC+TGG 0.567302 8.3:+19218707 MS.gene37227:CDS
GACATGACAAAACGTCCATC+CGG 0.567963 8.3:-19219676 None:intergenic
CCCATAGTTGGTCATAGGTA+GGG 0.571226 8.3:-19218322 None:intergenic
AAAGAGGAAAGGGATTATCA+AGG 0.572402 8.3:+19218872 MS.gene37227:CDS
TTGGGATACCGAAACAGGGC+AGG 0.573233 8.3:+19219198 MS.gene37227:CDS
GGCTACAGTTAGGGTTAGCA+TGG 0.574144 8.3:-19218243 None:intergenic
AGATTCTTATTTATAGCACA+AGG 0.575025 8.3:+19219575 MS.gene37227:CDS
GAAGGCATAGAGTGCATATG+TGG 0.575203 8.3:-19219499 None:intergenic
AAGAGTATGATCAGCATTTG+GGG 0.578509 8.3:+19219359 MS.gene37227:CDS
TGTGAAGCTTAACCCGGATG+AGG 0.580826 8.3:+19219258 MS.gene37227:CDS
TGTCACGAAGGCGTTACCAT+CGG 0.581186 8.3:+19219766 MS.gene37227:CDS
TAAATGTATGAGGACTTACA+TGG 0.581377 8.3:+19219090 MS.gene37227:CDS
AGCAGCGTTATTCTTCTGCA+AGG 0.583382 8.3:-19218534 None:intergenic
AGAATCCAGCTTCTGAGGCG+TGG 0.583927 8.3:+19218675 MS.gene37227:CDS
GACGAAAATAACGACGAAGA+GGG 0.591775 8.3:+19218641 MS.gene37227:CDS
ACTAATATACTTTATAACCA+CGG 0.592566 8.3:-19219473 None:intergenic
GCTAAAGACGGTTTAGCCCA+AGG 0.595353 8.3:+19218374 MS.gene37227:CDS
CTCGCCTTAGCATCTTTCGG+AGG 0.597269 8.3:-19218911 None:intergenic
GCTACTTTGCTCTGTTCCAG+AGG 0.598337 8.3:-19219802 None:intergenic
TGTTATGCTGTTCATCAAAG+AGG 0.601244 8.3:-19218448 None:intergenic
CCCTACCTATGACCAACTAT+GGG 0.603332 8.3:+19218322 MS.gene37227:CDS
TCTGTTCCAGAGGATGCCAC+TGG 0.603366 8.3:-19219792 None:intergenic
CATGGTGTCGTCGACTTTGG+GGG 0.604119 8.3:-19218225 None:intergenic
AGGAAAGGGATTATCAAGGT+AGG 0.608842 8.3:+19218876 MS.gene37227:CDS
CTAAACCGTCTTTAGCGTAA+GGG 0.612580 8.3:-19218367 None:intergenic
TTGGGGTGAAGCGAAATGGA+AGG 0.612660 8.3:-19219517 None:intergenic
TCTTTAGCGTAAGGGTGAGA+CGG 0.615894 8.3:-19218359 None:intergenic
GTCATGTCGGGAGATGGTGA+GGG 0.616926 8.3:+19219691 MS.gene37227:CDS
TTCAGTGGGATATGAATACA+GGG 0.624256 8.3:+19219326 MS.gene37227:CDS
AGGTAGGGTTGAAGGCGACA+AGG 0.626351 8.3:-19218307 None:intergenic
ACCATACTCCCATACCCTGA+GGG 0.627909 8.3:-19219446 None:intergenic
TAGTGATGACAAGTCCCTCA+GGG 0.628378 8.3:+19219432 MS.gene37227:CDS
CGACGAAAATAACGACGAAG+AGG 0.644376 8.3:+19218640 MS.gene37227:CDS
AGGATGCCACTGGCAACCGA+TGG 0.645652 8.3:-19219782 None:intergenic
TCTCACCCTTACGCTAAAGA+CGG 0.646041 8.3:+19218362 MS.gene37227:CDS
AGATTGGTGCATACTTGGAG+CGG 0.649087 8.3:+19218959 MS.gene37227:CDS
CCGATGTAATTTGACGCAGA+AGG 0.651218 8.3:-19218500 None:intergenic
GCGTTACCATCGGTTGCCAG+TGG 0.652062 8.3:+19219776 MS.gene37227:CDS
GATTCTTATTTATAGCACAA+GGG 0.653583 8.3:+19219576 MS.gene37227:CDS
GAGATGGTGAGGGTAAGTGT+TGG 0.656084 8.3:+19219701 MS.gene37227:CDS
CAGAGCAAAGTAGCGACGTG+TGG 0.656241 8.3:+19219811 MS.gene37227:CDS
GTGATAAGAAGATTGTTCAG+TGG 0.659340 8.3:+19219311 MS.gene37227:CDS
CCTTCTGCGTCAAATTACAT+CGG 0.670057 8.3:+19218500 MS.gene37227:CDS
AAATGTATGAGGACTTACAT+GGG 0.686630 8.3:+19219091 MS.gene37227:CDS
ATGGGAGTATGGTATTCCCG+TGG 0.688322 8.3:+19219456 MS.gene37227:CDS
TTGGAGCGGACATACAAAAG+GGG 0.692861 8.3:+19218973 MS.gene37227:CDS
AGAACTCTCAAGTGTCACGA+AGG 0.693137 8.3:+19219754 MS.gene37227:CDS
AGTATTGGGATACCGAAACA+GGG 0.695845 8.3:+19219194 MS.gene37227:CDS
TACCATACTCCCATACCCTG+AGG 0.704189 8.3:-19219447 None:intergenic
AGAGTATGATCAGCATTTGG+GGG 0.704600 8.3:+19219360 MS.gene37227:CDS
AACATAAGGGATCTTCCCAG+TGG 0.720474 8.3:-19219236 None:intergenic
AGATCAGAAGAAATACGCTG+AGG 0.728271 8.3:+19218763 MS.gene37227:CDS
CTGGTTATCCAAGCTCTGCG+CGG 0.754282 8.3:-19219554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAGAATATTAAGTAT+TGG + chr8.3:19219179-19219198 MS.gene37227:CDS 15.0%
!! TGATAAGAATATTAAGTATT+GGG + chr8.3:19219180-19219199 MS.gene37227:CDS 15.0%
!! ACTAATATACTTTATAACCA+CGG - chr8.3:19219476-19219495 None:intergenic 20.0%
!!! CATAACACCTTTTTAAAATT+CGG + chr8.3:19218464-19218483 MS.gene37227:CDS 20.0%
!!! GATATTTGTTTTACTAATGA+TGG + chr8.3:19219133-19219152 MS.gene37227:CDS 20.0%
! ATTCTCTTCTTTCTTCTTAA+GGG - chr8.3:19218609-19218628 None:intergenic 25.0%
! CTAATATACTTTATAACCAC+GGG - chr8.3:19219475-19219494 None:intergenic 25.0%
! GTTGTTATCAACAAAAGTAA+TGG - chr8.3:19219395-19219414 None:intergenic 25.0%
! TTCAACTTAACAAAAAGAAG+AGG + chr8.3:19219605-19219624 MS.gene37227:CDS 25.0%
! TTGTGCTATAAATAAGAATC+TGG - chr8.3:19219576-19219595 None:intergenic 25.0%
!! AGATTCTTATTTATAGCACA+AGG + chr8.3:19219575-19219594 MS.gene37227:CDS 25.0%
!! GATTCTTATTTATAGCACAA+GGG + chr8.3:19219576-19219595 MS.gene37227:CDS 25.0%
!!! TTACTTTTGTTGATAACAAC+AGG + chr8.3:19219395-19219414 MS.gene37227:CDS 25.0%
!!! TTGTTTTATACCAAAGAGAT+TGG + chr8.3:19218943-19218962 MS.gene37227:CDS 25.0%
AAATGTATGAGGACTTACAT+GGG + chr8.3:19219091-19219110 MS.gene37227:CDS 30.0%
CATTCTCTTCTTTCTTCTTA+AGG - chr8.3:19218610-19218629 None:intergenic 30.0%
GGATACTAAGGTTAAGATTT+GGG + chr8.3:19219051-19219070 MS.gene37227:CDS 30.0%
TAAATGTATGAGGACTTACA+TGG + chr8.3:19219090-19219109 MS.gene37227:CDS 30.0%
TGATAAGAAGATTGTTCAGT+GGG + chr8.3:19219312-19219331 MS.gene37227:CDS 30.0%
TGGATACTAAGGTTAAGATT+TGG + chr8.3:19219050-19219069 MS.gene37227:CDS 30.0%
! GGATAAACAGAATGTTTTGT+TGG + chr8.3:19219279-19219298 MS.gene37227:CDS 30.0%
!! AGCGTAACCGAATTTTAAAA+AGG - chr8.3:19218474-19218493 None:intergenic 30.0%
!!! AAGGATAAGAGTACTTTTCA+TGG + chr8.3:19218848-19218867 MS.gene37227:CDS 30.0%
!!! AGGATAAGAGTACTTTTCAT+GGG + chr8.3:19218849-19218868 MS.gene37227:CDS 30.0%
!!! ATTAGGTTTTTTCCGAATTC+GGG + chr8.3:19219004-19219023 MS.gene37227:CDS 30.0%
!!! GATTAGGTTTTTTCCGAATT+CGG + chr8.3:19219003-19219022 MS.gene37227:CDS 30.0%
AAAGAGGAAAGGGATTATCA+AGG + chr8.3:19218872-19218891 MS.gene37227:CDS 35.0%
AACAAAAAGAAGAGGTTTGC+TGG + chr8.3:19219613-19219632 MS.gene37227:CDS 35.0%
AAGAAAGGCGAAGAAAAAAG+TGG + chr8.3:19218797-19218816 MS.gene37227:CDS 35.0%
ACAAATATCCCTAACAGCTT+TGG - chr8.3:19219122-19219141 None:intergenic 35.0%
ACATTCTGTTTATCCTCATC+CGG - chr8.3:19219274-19219293 None:intergenic 35.0%
AGAATCAAATGACCCGAATT+CGG - chr8.3:19219019-19219038 None:intergenic 35.0%
AGGTTTACAAGGAGAGTTAA+CGG + chr8.3:19218739-19218758 MS.gene37227:CDS 35.0%
GAAGAATAAGAAGAGTCCTT+GGG + chr8.3:19218703-19218722 MS.gene37227:CDS 35.0%
GGTTAAGCTTCACAACATAA+GGG - chr8.3:19219252-19219271 None:intergenic 35.0%
GTGATAAGAAGATTGTTCAG+TGG + chr8.3:19219311-19219330 MS.gene37227:CDS 35.0%
TCAACACTGGTAAATGTATG+AGG + chr8.3:19219080-19219099 MS.gene37227:CDS 35.0%
TGAAAAAGTTGAGGCTGTTA+AGG + chr8.3:19218829-19218848 MS.gene37227:CDS 35.0%
TGAAGAATAAGAAGAGTCCT+TGG + chr8.3:19218702-19218721 MS.gene37227:CDS 35.0%
TGTTATGCTGTTCATCAAAG+AGG - chr8.3:19218451-19218470 None:intergenic 35.0%
TTATGTTGTGAAGCTTAACC+CGG + chr8.3:19219252-19219271 MS.gene37227:CDS 35.0%
TTCAGTGGGATATGAATACA+GGG + chr8.3:19219326-19219345 MS.gene37227:CDS 35.0%
! AAGAGTATGATCAGCATTTG+GGG + chr8.3:19219359-19219378 MS.gene37227:CDS 35.0%
! CAAGAGTATGATCAGCATTT+GGG + chr8.3:19219358-19219377 MS.gene37227:CDS 35.0%
! TCAAGAGTATGATCAGCATT+TGG + chr8.3:19219357-19219376 MS.gene37227:CDS 35.0%
! TTTTCATGGGAAAGAGGAAA+GGG + chr8.3:19218862-19218881 MS.gene37227:CDS 35.0%
! TTTTTCTTCGCCTTTCTTCT+TGG - chr8.3:19218795-19218814 None:intergenic 35.0%
!! AAACAGAATGTTTTGTTGGC+TGG + chr8.3:19219283-19219302 MS.gene37227:CDS 35.0%
!! ATTTGGGATGTTTTCAACAC+TGG + chr8.3:19219067-19219086 MS.gene37227:CDS 35.0%
!!! GAAGAAAAAAGTGGTTTTGG+TGG + chr8.3:19218806-19218825 MS.gene37227:CDS 35.0%
!!! TTTTGGTGGTGAAAAAGTTG+AGG + chr8.3:19218820-19218839 MS.gene37227:CDS 35.0%
AACTCAAAACCCTGATTCAC+CGG + chr8.3:19218163-19218182 MS.gene37227:CDS 40.0%
AGAGTATGATCAGCATTTGG+GGG + chr8.3:19219360-19219379 MS.gene37227:CDS 40.0%
AGATCAGAAGAAATACGCTG+AGG + chr8.3:19218763-19218782 MS.gene37227:CDS 40.0%
AGGAAAGGGATTATCAAGGT+AGG + chr8.3:19218876-19218895 MS.gene37227:CDS 40.0%
CAAAGAGATTGGTGCATACT+TGG + chr8.3:19218954-19218973 MS.gene37227:CDS 40.0%
CAAGTATGCACCAATCTCTT+TGG - chr8.3:19218956-19218975 None:intergenic 40.0%
CATTCTGTTTATCCTCATCC+GGG - chr8.3:19219273-19219292 None:intergenic 40.0%
CCTTCTGCGTCAAATTACAT+CGG + chr8.3:19218500-19218519 MS.gene37227:CDS 40.0%
CTAAACCGTCTTTAGCGTAA+GGG - chr8.3:19218370-19218389 None:intergenic 40.0%
GACGAAAATAACGACGAAGA+GGG + chr8.3:19218641-19218660 MS.gene37227:CDS 40.0%
GGGTTAAGCTTCACAACATA+AGG - chr8.3:19219253-19219272 None:intergenic 40.0%
GTTCAGTGGGATATGAATAC+AGG + chr8.3:19219325-19219344 MS.gene37227:CDS 40.0%
TGCTGTTCATCAAAGAGGAA+AGG - chr8.3:19218446-19218465 None:intergenic 40.0%
TTACTCGCCTTAGCATCTTT+CGG - chr8.3:19218917-19218936 None:intergenic 40.0%
TTATATCGACGTTCTCCACT+GGG + chr8.3:19219221-19219240 MS.gene37227:CDS 40.0%
TTCGGGTCATTTGATTCTCT+CGG + chr8.3:19219021-19219040 MS.gene37227:CDS 40.0%
TTTGTGGTTACGCATACCTT+GGG - chr8.3:19218393-19218412 None:intergenic 40.0%
! AGTATTGGGATACCGAAACA+GGG + chr8.3:19219194-19219213 MS.gene37227:CDS 40.0%
! ATGGGTTTAGAGAGGGTTTT+GGG - chr8.3:19218272-19218291 None:intergenic 40.0%
! CTTTTCATGGGAAAGAGGAA+AGG + chr8.3:19218861-19218880 MS.gene37227:CDS 40.0%
! GAGAATTTTCCGGTGAATCA+GGG - chr8.3:19218175-19218194 None:intergenic 40.0%
! GAGTACTTTTCATGGGAAAG+AGG + chr8.3:19218856-19218875 MS.gene37227:CDS 40.0%
! GGCGAAGAAAAAAGTGGTTT+TGG + chr8.3:19218803-19218822 MS.gene37227:CDS 40.0%
! TTGTCAGGTCAATTTTTCGC+CGG + chr8.3:19219657-19219676 MS.gene37227:CDS 40.0%
! TTTTCCTCTTCTTCTGTTCG+TGG - chr8.3:19218580-19218599 None:intergenic 40.0%
!! AAGTATTGGGATACCGAAAC+AGG + chr8.3:19219193-19219212 MS.gene37227:CDS 40.0%
!!! GGAACAAAGTTTTTGAGTGC+TGG + chr8.3:19219154-19219173 MS.gene37227:CDS 40.0%
AACATAAGGGATCTTCCCAG+TGG - chr8.3:19219239-19219258 None:intergenic 45.0%
AATAAGAAGAGTCCTTGGGC+TGG + chr8.3:19218707-19218726 MS.gene37227:CDS 45.0%
ACCCTACCTATGACCAACTA+TGG + chr8.3:19218321-19218340 MS.gene37227:CDS 45.0%
ACTTGGAGCGGACATACAAA+AGG + chr8.3:19218971-19218990 MS.gene37227:CDS 45.0%
AGAACTCTCAAGTGTCACGA+AGG + chr8.3:19219754-19219773 MS.gene37227:CDS 45.0%
AGATTGGTGCATACTTGGAG+CGG + chr8.3:19218959-19218978 MS.gene37227:CDS 45.0%
AGCAGCGTTATTCTTCTGCA+AGG - chr8.3:19218537-19218556 None:intergenic 45.0%
ATAAGAAGAGTCCTTGGGCT+GGG + chr8.3:19218708-19218727 MS.gene37227:CDS 45.0%
CAAAAGGGGTTTCTGCGATT+AGG + chr8.3:19218987-19219006 MS.gene37227:CDS 45.0%
CCCATAGTTGGTCATAGGTA+GGG - chr8.3:19218325-19218344 None:intergenic 45.0%
CCCTACCTATGACCAACTAT+GGG + chr8.3:19218322-19218341 MS.gene37227:CDS 45.0%
CCGATGTAATTTGACGCAGA+AGG - chr8.3:19218503-19218522 None:intergenic 45.0%
CGACGAAAATAACGACGAAG+AGG + chr8.3:19218640-19218659 MS.gene37227:CDS 45.0%
CGATATAACCTGCCCTGTTT+CGG - chr8.3:19219209-19219228 None:intergenic 45.0%
CTTCGACGAAACCAAGTTTG+TGG - chr8.3:19218409-19218428 None:intergenic 45.0%
CTTGGAGCGGACATACAAAA+GGG + chr8.3:19218972-19218991 MS.gene37227:CDS 45.0%
GACATGACAAAACGTCCATC+CGG - chr8.3:19219679-19219698 None:intergenic 45.0%
GATTGAGAATCCAGCTTCTG+AGG + chr8.3:19218670-19218689 MS.gene37227:CDS 45.0%
GCTAAACCGTCTTTAGCGTA+AGG - chr8.3:19218371-19218390 None:intergenic 45.0%
GGGAAGAAGGAAGGTTTACA+AGG + chr8.3:19218728-19218747 MS.gene37227:CDS 45.0%
GGGATTATCAAGGTAGGTCT+TGG + chr8.3:19218882-19218901 MS.gene37227:CDS 45.0%
GGGTCATTCCAAAGCTGTTA+GGG + chr8.3:19219111-19219130 MS.gene37227:CDS 45.0%
GGTATGCGTAACCACAAACT+TGG + chr8.3:19218395-19218414 MS.gene37227:CDS 45.0%
GTTATATCGACGTTCTCCAC+TGG + chr8.3:19219220-19219239 MS.gene37227:CDS 45.0%
GTTTGTGGTTACGCATACCT+TGG - chr8.3:19218394-19218413 None:intergenic 45.0%
TAGTGATGACAAGTCCCTCA+GGG + chr8.3:19219432-19219451 MS.gene37227:CDS 45.0%
TCTCACCCTTACGCTAAAGA+CGG + chr8.3:19218362-19218381 MS.gene37227:CDS 45.0%
TCTTCTTCTGTTCGTGGTGT+GGG - chr8.3:19218574-19218593 None:intergenic 45.0%
TCTTTAGCGTAAGGGTGAGA+CGG - chr8.3:19218362-19218381 None:intergenic 45.0%
TGGGTCATTCCAAAGCTGTT+AGG + chr8.3:19219110-19219129 MS.gene37227:CDS 45.0%
TGTTGCTGGATATGCTTGTC+AGG + chr8.3:19219642-19219661 MS.gene37227:CDS 45.0%
TTGGAGCGGACATACAAAAG+GGG + chr8.3:19218973-19218992 MS.gene37227:CDS 45.0%
TTGGTCATAGGTAGGGTTGA+AGG - chr8.3:19218318-19218337 None:intergenic 45.0%
TTTGATTCTCTCGGCTGGTA+TGG + chr8.3:19219030-19219049 MS.gene37227:CDS 45.0%
TTTGCTGGACACATTGTTGC+TGG + chr8.3:19219628-19219647 MS.gene37227:CDS 45.0%
! GAAGGCATAGAGTGCATATG+TGG - chr8.3:19219502-19219521 None:intergenic 45.0%
! GATGGACGTTTTGTCATGTC+GGG + chr8.3:19219679-19219698 MS.gene37227:CDS 45.0%
! GGAGAATTTTCCGGTGAATC+AGG - chr8.3:19218176-19218195 None:intergenic 45.0%
! GGATGGACGTTTTGTCATGT+CGG + chr8.3:19219678-19219697 MS.gene37227:CDS 45.0%
! TGGGTTTAGAGAGGGTTTTG+GGG - chr8.3:19218271-19218290 None:intergenic 45.0%
!! GATGGGTTTAGAGAGGGTTT+TGG - chr8.3:19218273-19218292 None:intergenic 45.0%
!! GTAAGTGTTGGTTCTGGGAT+TGG + chr8.3:19219713-19219732 MS.gene37227:CDS 45.0%
!! TGAGGGTAAGTGTTGGTTCT+GGG + chr8.3:19219708-19219727 MS.gene37227:CDS 45.0%
!!! GGTGAATCAGGGTTTTGAGT+TGG - chr8.3:19218164-19218183 None:intergenic 45.0%
!!! TTTAGAGAGGGTTTTGGGGT+CGG - chr8.3:19218267-19218286 None:intergenic 45.0%
AATAACCACGCCTCAGAAGC+TGG - chr8.3:19218683-19218702 None:intergenic 50.0%
ACCATACTCCCATACCCTGA+GGG - chr8.3:19219449-19219468 None:intergenic 50.0%
ATGACAAGTCCCTCAGGGTA+TGG + chr8.3:19219437-19219456 MS.gene37227:CDS 50.0%
CAATACTCCGACAACGATCC+AGG + chr8.3:19218134-19218153 MS.gene37227:CDS 50.0%
CACACCACGAACAGAAGAAG+AGG + chr8.3:19218573-19218592 MS.gene37227:CDS 50.0%
CTAGTGATGACAAGTCCCTC+AGG + chr8.3:19219431-19219450 MS.gene37227:CDS 50.0%
CTCGGCTGGTATGGATACTA+AGG + chr8.3:19219039-19219058 MS.gene37227:CDS 50.0%
CTCTTCTTCTGTTCGTGGTG+TGG - chr8.3:19218575-19218594 None:intergenic 50.0%
CTGGATCGTTGTCGGAGTAT+TGG - chr8.3:19218136-19218155 None:intergenic 50.0%
CTTTAGCGTAAGGGTGAGAC+GGG - chr8.3:19218361-19218380 None:intergenic 50.0%
GAGATGGTGAGGGTAAGTGT+TGG + chr8.3:19219701-19219720 MS.gene37227:CDS 50.0%
GCCCATAGTTGGTCATAGGT+AGG - chr8.3:19218326-19218345 None:intergenic 50.0%
GCTAAAGACGGTTTAGCCCA+AGG + chr8.3:19218374-19218393 MS.gene37227:CDS 50.0%
GGTCATTTGATTCTCTCGGC+TGG + chr8.3:19219025-19219044 MS.gene37227:CDS 50.0%
TACCATACTCCCATACCCTG+AGG - chr8.3:19219450-19219469 None:intergenic 50.0%
TGACAAGTCCCTCAGGGTAT+GGG + chr8.3:19219438-19219457 MS.gene37227:CDS 50.0%
TGGTGAACCTGGATCGTTGT+CGG - chr8.3:19218144-19218163 None:intergenic 50.0%
TGTGAAGCTTAACCCGGATG+AGG + chr8.3:19219258-19219277 MS.gene37227:CDS 50.0%
TTGGGGTGAAGCGAAATGGA+AGG - chr8.3:19219520-19219539 None:intergenic 50.0%
! AGACGGGGTGGAGAATTTTC+CGG - chr8.3:19218185-19218204 None:intergenic 50.0%
! ATGGGAGTATGGTATTCCCG+TGG + chr8.3:19219456-19219475 MS.gene37227:CDS 50.0%
! CAGGTCAATTTTTCGCCGGA+TGG + chr8.3:19219661-19219680 MS.gene37227:CDS 50.0%
! CGTTTTGTCATGTCGGGAGA+TGG + chr8.3:19219685-19219704 MS.gene37227:CDS 50.0%
! GCTACTTTGCTCTGTTCCAG+AGG - chr8.3:19219805-19219824 None:intergenic 50.0%
! GGCTACAGTTAGGGTTAGCA+TGG - chr8.3:19218246-19218265 None:intergenic 50.0%
! TGGGTCGATGGGTTTAGAGA+GGG - chr8.3:19218279-19218298 None:intergenic 50.0%
! TTGGGTCGATGGGTTTAGAG+AGG - chr8.3:19218280-19218299 None:intergenic 50.0%
! TTTGGGGTCGGCTACAGTTA+GGG - chr8.3:19218255-19218274 None:intergenic 50.0%
!! AAGGCGACAAGGTGTTGAGT+TGG - chr8.3:19218299-19218318 None:intergenic 50.0%
!! AAGGTGTTGAGTTGGGTCGA+TGG - chr8.3:19218291-19218310 None:intergenic 50.0%
!! AGCATGGTGTCGTCGACTTT+GGG - chr8.3:19218230-19218249 None:intergenic 50.0%
!! AGGCGACAAGGTGTTGAGTT+GGG - chr8.3:19218298-19218317 None:intergenic 50.0%
!! AGGTGTTGAGTTGGGTCGAT+GGG - chr8.3:19218290-19218309 None:intergenic 50.0%
!! GGGACGGGTTGATAAAGTAC+TGG + chr8.3:19219836-19219855 MS.gene37227:CDS 50.0%
!! GTGAGGGTAAGTGTTGGTTC+TGG + chr8.3:19219707-19219726 MS.gene37227:CDS 50.0%
!! TAGCATGGTGTCGTCGACTT+TGG - chr8.3:19218231-19218250 None:intergenic 50.0%
!! TGTCACGAAGGCGTTACCAT+CGG + chr8.3:19219766-19219785 MS.gene37227:CDS 50.0%
!!! GGGTTTTGAGTTGGTGAACC+TGG - chr8.3:19218155-19218174 None:intergenic 50.0%
!!! TTTTGGGGTCGGCTACAGTT+AGG - chr8.3:19218256-19218275 None:intergenic 50.0%
AGGTAGGGTTGAAGGCGACA+AGG - chr8.3:19218310-19218329 None:intergenic 55.0%
CAAAGTAGCGACGTGTGGCT+GGG + chr8.3:19219816-19219835 MS.gene37227:CDS 55.0%
CAACTATGGGCTCCCATCCA+AGG + chr8.3:19218335-19218354 MS.gene37227:CDS 55.0%
CAGAGCAAAGTAGCGACGTG+TGG + chr8.3:19219811-19219830 MS.gene37227:CDS 55.0%
CTCGCCTTAGCATCTTTCGG+AGG - chr8.3:19218914-19218933 None:intergenic 55.0%
CTGGTTATCCAAGCTCTGCG+CGG - chr8.3:19219557-19219576 None:intergenic 55.0%
CTTGGATGGGAGCCCATAGT+TGG - chr8.3:19218337-19218356 None:intergenic 55.0%
GGGAGCCCATAGTTGGTCAT+AGG - chr8.3:19218330-19218349 None:intergenic 55.0%
GTCATGTCGGGAGATGGTGA+GGG + chr8.3:19219691-19219710 MS.gene37227:CDS 55.0%
TCCCTCAGGGTATGGGAGTA+TGG + chr8.3:19219445-19219464 MS.gene37227:CDS 55.0%
TCTGTTCCAGAGGATGCCAC+TGG - chr8.3:19219795-19219814 None:intergenic 55.0%
TGTCATGTCGGGAGATGGTG+AGG + chr8.3:19219690-19219709 MS.gene37227:CDS 55.0%
TTGGGATACCGAAACAGGGC+AGG + chr8.3:19219198-19219217 MS.gene37227:CDS 55.0%
! GAGGAGCACGCCAAGAAGAA+AGG + chr8.3:19218782-19218801 MS.gene37227:CDS 55.0%
!! AGAATCCAGCTTCTGAGGCG+TGG + chr8.3:19218675-19218694 MS.gene37227:CDS 55.0%
!! CATGGTGTCGTCGACTTTGG+GGG - chr8.3:19218228-19218247 None:intergenic 55.0%
!! GCATGGTGTCGTCGACTTTG+GGG - chr8.3:19218229-19218248 None:intergenic 55.0%
AGAACGGGATGGGAGGAGAC+GGG - chr8.3:19218201-19218220 None:intergenic 60.0%
AGGATGCCACTGGCAACCGA+TGG - chr8.3:19219785-19219804 None:intergenic 60.0%
CAGAACGGGATGGGAGGAGA+CGG - chr8.3:19218202-19218221 None:intergenic 60.0%
CCTTCCTTCTTCCCAGCCCA+AGG - chr8.3:19218722-19218741 None:intergenic 60.0%
CGCGCCTCCGAAAGATGCTA+AGG + chr8.3:19218907-19218926 MS.gene37227:CDS 60.0%
CGCGTTGGGGTGAAGCGAAA+TGG - chr8.3:19219524-19219543 None:intergenic 60.0%
CGCTTCACCCCAACGCGAAT+TGG + chr8.3:19219527-19219546 MS.gene37227:CDS 60.0%
GCAAAGTAGCGACGTGTGGC+TGG + chr8.3:19219815-19219834 MS.gene37227:CDS 60.0%
! CCTTGGGCTGGGAAGAAGGA+AGG + chr8.3:19218719-19218738 MS.gene37227:CDS 60.0%
! GAGTCCTTGGGCTGGGAAGA+AGG + chr8.3:19218715-19218734 MS.gene37227:CDS 60.0%
! GCGTTACCATCGGTTGCCAG+TGG + chr8.3:19219776-19219795 MS.gene37227:CDS 60.0%
AGGGTGAGACGGGCCTTGGA+TGG - chr8.3:19218351-19218370 None:intergenic 65.0%
CGGCGAGCCAATTCGCGTTG+GGG - chr8.3:19219537-19219556 None:intergenic 65.0%
CGGTTGCCAGTGGCATCCTC+TGG + chr8.3:19219786-19219805 MS.gene37227:CDS 65.0%
CGTAAGGGTGAGACGGGCCT+TGG - chr8.3:19218355-19218374 None:intergenic 65.0%
GAACGGGATGGGAGGAGACG+GGG - chr8.3:19218200-19218219 None:intergenic 65.0%
GCGGCGAGCCAATTCGCGTT+GGG - chr8.3:19219538-19219557 None:intergenic 65.0%
GGGTGAGACGGGCCTTGGAT+GGG - chr8.3:19218350-19218369 None:intergenic 65.0%
GTAGCGACGTGTGGCTGGGA+CGG + chr8.3:19219820-19219839 MS.gene37227:CDS 65.0%
TAGCGACGTGTGGCTGGGAC+GGG + chr8.3:19219821-19219840 MS.gene37227:CDS 65.0%
! CGACTTTGGGGGCGGCAGAA+CGG - chr8.3:19218217-19218236 None:intergenic 65.0%
! GACTTTGGGGGCGGCAGAAC+GGG - chr8.3:19218216-19218235 None:intergenic 65.0%
!! GGTGTCGTCGACTTTGGGGG+CGG - chr8.3:19218225-19218244 None:intergenic 65.0%
CGCGGCGAGCCAATTCGCGT+TGG - chr8.3:19219539-19219558 None:intergenic 70.0%
TGGGGGCGGCAGAACGGGAT+GGG - chr8.3:19218211-19218230 None:intergenic 70.0%
TTGGGGGCGGCAGAACGGGA+TGG - chr8.3:19218212-19218231 None:intergenic 70.0%
! TTGGCTCGCCGCGCAGAGCT+TGG + chr8.3:19219546-19219565 MS.gene37227:CDS 70.0%
CGGGATGGGAGGAGACGGGG+TGG - chr8.3:19218197-19218216 None:intergenic 75.0%
GGGCGGCAGAACGGGATGGG+AGG - chr8.3:19218208-19218227 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 19218119 19219861 19218119 ID=MS.gene37227
chr8.3 mRNA 19218119 19219861 19218119 ID=MS.gene37227.t1;Parent=MS.gene37227
chr8.3 exon 19218119 19219861 19218119 ID=MS.gene37227.t1.exon1;Parent=MS.gene37227.t1
chr8.3 CDS 19218119 19219861 19218119 ID=cds.MS.gene37227.t1;Parent=MS.gene37227.t1
Gene Sequence

>MS.gene37227

ATGGATCTACTCAACCAATACTCCGACAACGATCCAGGTTCACCAACTCAAAACCCTGATTCACCGGAAAATTCTCCACCCCGTCTCCTCCCATCCCGTTCTGCCGCCCCCAAAGTCGACGACACCATGCTAACCCTAACTGTAGCCGACCCCAAAACCCTCTCTAAACCCATCGACCCAACTCAACACCTTGTCGCCTTCAACCCTACCTATGACCAACTATGGGCTCCCATCCAAGGCCCGTCTCACCCTTACGCTAAAGACGGTTTAGCCCAAGGTATGCGTAACCACAAACTTGGTTTCGTCGAAGATGCTAACATTGAACCTTTCCTCTTTGATGAACAGCATAACACCTTTTTAAAATTCGGTTACGCTGCTGATCCTTCTGCGTCAAATTACATCGGTGATTTCGATGCCTTGCAGAAGAATAACGCTGCTTCTGTTTACAATATCCCACACCACGAACAGAAGAAGAGGAAAATTGAATCCCTTAAGAAGAAAGAAGAGAATGATGATGAAGACGACGAAAATAACGACGAAGAGGGCGTTGAGATTGAGAATCCAGCTTCTGAGGCGTGGTTATTGAAGAATAAGAAGAGTCCTTGGGCTGGGAAGAAGGAAGGTTTACAAGGAGAGTTAACGGAAGATCAGAAGAAATACGCTGAGGAGCACGCCAAGAAGAAAGGCGAAGAAAAAAGTGGTTTTGGTGGTGAAAAAGTTGAGGCTGTTAAGGATAAGAGTACTTTTCATGGGAAAGAGGAAAGGGATTATCAAGGTAGGTCTTGGATCGCGCCTCCGAAAGATGCTAAGGCGAGTAATGATCATTGTTTTATACCAAAGAGATTGGTGCATACTTGGAGCGGACATACAAAAGGGGTTTCTGCGATTAGGTTTTTTCCGAATTCGGGTCATTTGATTCTCTCGGCTGGTATGGATACTAAGGTTAAGATTTGGGATGTTTTCAACACTGGTAAATGTATGAGGACTTACATGGGTCATTCCAAAGCTGTTAGGGATATTTGTTTTACTAATGATGGAACAAAGTTTTTGAGTGCTGGTTATGATAAGAATATTAAGTATTGGGATACCGAAACAGGGCAGGTTATATCGACGTTCTCCACTGGGAAGATCCCTTATGTTGTGAAGCTTAACCCGGATGAGGATAAACAGAATGTTTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATACAGGGCAGATAACTCAAGAGTATGATCAGCATTTGGGGGCTGTCAATACCATTACTTTTGTTGATAACAACAGGAGATTTGTTACTTCTAGTGATGACAAGTCCCTCAGGGTATGGGAGTATGGTATTCCCGTGGTTATAAAGTATATTAGTGAGCCACATATGCACTCTATGCCTTCCATTTCGCTTCACCCCAACGCGAATTGGCTCGCCGCGCAGAGCTTGGATAACCAGATTCTTATTTATAGCACAAGGGAGAAGTTTCAACTTAACAAAAAGAAGAGGTTTGCTGGACACATTGTTGCTGGATATGCTTGTCAGGTCAATTTTTCGCCGGATGGACGTTTTGTCATGTCGGGAGATGGTGAGGGTAAGTGTTGGTTCTGGGATTGGAAGAGTTGCAAAGTCTTTAGAACTCTCAAGTGTCACGAAGGCGTTACCATCGGTTGCCAGTGGCATCCTCTGGAACAGAGCAAAGTAGCGACGTGTGGCTGGGACGGGTTGATAAAGTACTGGTAA
Protein sequence

>MS.gene37227.t1

MDLLNQYSDNDPGSPTQNPDSPENSPPRLLPSRSAAPKVDDTMLTLTVADPKTLSKPIDPTQHLVAFNPTYDQLWAPIQGPSHPYAKDGLAQGMRNHKLGFVEDANIEPFLFDEQHNTFLKFGYAADPSASNYIGDFDALQKNNAASVYNIPHHEQKKRKIESLKKKEENDDEDDENNDEEGVEIENPASEAWLLKNKKSPWAGKKEGLQGELTEDQKKYAEEHAKKKGEEKSGFGGEKVEAVKDKSTFHGKEERDYQGRSWIAPPKDAKASNDHCFIPKRLVHTWSGHTKGVSAIRFFPNSGHLILSAGMDTKVKIWDVFNTGKCMRTYMGHSKAVRDICFTNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDEDKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEYGIPVVIKYISEPHMHSMPSISLHPNANWLAAQSLDNQILIYSTREKFQLNKKKRFAGHIVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVTIGCQWHPLEQSKVATCGWDGLIKYW