Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38101.t1 | XP_003618014.1 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 1.40E-15 | 93.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38101.t1 | G7K500 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 1.0e-15 | 93.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051648 | MS.gene38101 | 0.827962 | 1.17E-54 | -1.69E-46 |
| MS.gene052150 | MS.gene38101 | 0.830844 | 2.34E-55 | -1.69E-46 |
| MS.gene052151 | MS.gene38101 | 0.816546 | 5.23E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38101.t1 | MTR_5g098020 | 91.795 | 195 | 16 | 0 | 1 | 195 | 1 | 195 | 2.57e-81 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTGAAGATTCTTCTT+TGG | 0.248746 | 5.1:+78358070 | None:intergenic |
| CCAATTGAGAAGAGTGAATA+AGG | 0.253557 | 5.1:-78357633 | None:intergenic |
| CCTTATTCACTCTTCTCAAT+TGG | 0.285248 | 5.1:+78357633 | None:intergenic |
| TTCTGTTGTTGAGGCTGTTC+AGG | 0.288375 | 5.1:-78357868 | MS.gene38101:CDS |
| TCTGATGCTGCTGGTGTGAT+TGG | 0.302513 | 5.1:+78358095 | None:intergenic |
| GTCGAGGAAACAAAAGAAAA+TGG | 0.336894 | 5.1:-78357894 | MS.gene38101:CDS |
| CTCAACAGGTTCCTTTGTCT+CGG | 0.374352 | 5.1:+78357947 | None:intergenic |
| TCTGTCTCAGATTCTTCAGC+TGG | 0.392509 | 5.1:+78357840 | None:intergenic |
| TGAGGAGAAGGTTGAGAAAG+AGG | 0.398472 | 5.1:-78357733 | MS.gene38101:CDS |
| TGGACTCTCAATGATTTCTT+CGG | 0.404920 | 5.1:+78358115 | None:intergenic |
| CTCAGCTTTAAGCAAAGGTT+TGG | 0.421527 | 5.1:+78358043 | None:intergenic |
| GGTTGAGAAAGAGGAAGCCT+TGG | 0.426099 | 5.1:-78357724 | MS.gene38101:CDS |
| TGAGACAGAAAATGTTGTTA+AGG | 0.433126 | 5.1:-78357826 | MS.gene38101:CDS |
| GGTAGCTGAGGCTGAAACTA+AGG | 0.438659 | 5.1:-78357793 | MS.gene38101:CDS |
| TCTTTGGTTTCTGATGCTGC+TGG | 0.462673 | 5.1:+78358086 | None:intergenic |
| GCTTAAAGCTGAGGCAAAAG+AGG | 0.486761 | 5.1:-78358033 | MS.gene38101:CDS |
| CAATTGAGAAGAGTGAATAA+GGG | 0.495888 | 5.1:-78357632 | None:intergenic |
| TCCAATTATGGCCACTGTTG+AGG | 0.502667 | 5.1:-78358912 | None:intergenic |
| ACTCTCAATGATTTCTTCGG+TGG | 0.520132 | 5.1:+78358118 | None:intergenic |
| TACAGCAGAGGAAACAATGA+AGG | 0.533083 | 5.1:-78357979 | MS.gene38101:CDS |
| GTTGAGGAAACAAAAGAAAA+CGG | 0.538839 | 5.1:-78357930 | MS.gene38101:CDS |
| AGAAGAGAAAAGTGAGGAGA+AGG | 0.539879 | 5.1:-78357745 | MS.gene38101:CDS |
| TTTGCCTCAGCTTTAAGCAA+AGG | 0.549317 | 5.1:+78358038 | None:intergenic |
| AATTGAGAAGAATGAAGAAG+AGG | 0.561646 | 5.1:-78357658 | MS.gene38101:CDS |
| GGAAGAACAAACCGAGACAA+AGG | 0.568414 | 5.1:-78357958 | MS.gene38101:CDS |
| CAAACCTTTGCTTAAAGCTG+AGG | 0.572895 | 5.1:-78358042 | MS.gene38101:CDS |
| CGAGACAAAGGAACCTGTTG+AGG | 0.574462 | 5.1:-78357946 | MS.gene38101:CDS |
| AGCCTTGGATGAAAAGAAAG+AGG | 0.599026 | 5.1:-78357709 | MS.gene38101:CDS |
| AAATGGTGATTCTGTTGTTG+AGG | 0.613322 | 5.1:-78357877 | MS.gene38101:CDS |
| TGTTGTTAAGGATGAGAATG+TGG | 0.621253 | 5.1:-78357814 | MS.gene38101:CDS |
| TGATAACTGAGCAGTGTCGT+CGG | 0.622323 | 5.1:+78358160 | None:intergenic |
| CGGTGATTCAGTTAATGTCG+AGG | 0.622446 | 5.1:-78357910 | MS.gene38101:CDS |
| AGCCTCAGAAGAGAAAAGTG+AGG | 0.660719 | 5.1:-78357751 | MS.gene38101:CDS |
| GGATGAGAATGTGGTAGCTG+AGG | 0.670593 | 5.1:-78357805 | MS.gene38101:CDS |
| TAAAGCTGAGGCAAAAGAGG+TGG | 0.674001 | 5.1:-78358030 | MS.gene38101:CDS |
| GGTGGTAGAAGAAGTTCTTG+TGG | 0.675484 | 5.1:-78358012 | MS.gene38101:CDS |
| GGCTGTGAAAGATACAGCAG+AGG | 0.684792 | 5.1:-78357991 | MS.gene38101:CDS |
| TGGTAGAGTTACCTCAACAG+TGG | 0.712754 | 5.1:+78358901 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGAAATATTAATTATAAT+TGG | - | chr5.1:78358016-78358035 | MS.gene38101:CDS | 10.0% |
| !!! | AATCAATGTTTAAAATCAAA+TGG | + | chr5.1:78357711-78357730 | None:intergenic | 15.0% |
| !!! | AATTTTGAGTCTTTTATTTT+TGG | - | chr5.1:78358122-78358141 | MS.gene38101:CDS | 15.0% |
| !! | AAAAATTGAAGCTCTTAAAA+TGG | - | chr5.1:78357997-78358016 | MS.gene38101:CDS | 20.0% |
| !! | AGATAATTTATCCTTATGAA+CGG | + | chr5.1:78358160-78358179 | None:intergenic | 20.0% |
| !!! | AATATTTTAAAGCATCTAGT+AGG | - | chr5.1:78357745-78357764 | MS.gene38101:CDS | 20.0% |
| !!! | GAACAAAATTTGTTTTAGAT+AGG | - | chr5.1:78358215-78358234 | MS.gene38101:intron | 20.0% |
| !!! | TTTACTTTTTAATCGAACTT+TGG | + | chr5.1:78358191-78358210 | None:intergenic | 20.0% |
| ! | AAAAACCAAGTACTTGAAAT+TGG | + | chr5.1:78357662-78357681 | None:intergenic | 25.0% |
| ! | AATCACATTGTGATTCATAT+TGG | - | chr5.1:78357834-78357853 | MS.gene38101:CDS | 25.0% |
| ! | GATAATTTATCCTTATGAAC+GGG | + | chr5.1:78358159-78358178 | None:intergenic | 25.0% |
| ! | TCATATCTTCTTGAGTAAAT+AGG | + | chr5.1:78357970-78357989 | None:intergenic | 25.0% |
| ! | TTTCAATACTCTGACTAATA+TGG | + | chr5.1:78358094-78358113 | None:intergenic | 25.0% |
| !! | CAAATGTTGTTTATTAGACA+AGG | + | chr5.1:78358308-78358327 | None:intergenic | 25.0% |
| !!! | ACCTTTTTTAATGCATTAGA+TGG | - | chr5.1:78357935-78357954 | MS.gene38101:CDS | 25.0% |
| !!! | TCAATATTTTAATGTCTCTC+CGG | + | chr5.1:78357913-78357932 | None:intergenic | 25.0% |
| AATTGAGAAGAATGAAGAAG+AGG | - | chr5.1:78358882-78358901 | MS.gene38101:intron | 30.0% | |
| ATCTTCTTGAGTAAATAGGA+AGG | + | chr5.1:78357966-78357985 | None:intergenic | 30.0% | |
| ATGTGCATGATTAATTCATG+TGG | + | chr5.1:78358060-78358079 | None:intergenic | 30.0% | |
| GCCATCTAATGCATTAAAAA+AGG | + | chr5.1:78357939-78357958 | None:intergenic | 30.0% | |
| GTTGAGGAAACAAAAGAAAA+CGG | - | chr5.1:78358610-78358629 | MS.gene38101:intron | 30.0% | |
| TGAGACAGAAAATGTTGTTA+AGG | - | chr5.1:78358714-78358733 | MS.gene38101:intron | 30.0% | |
| ! | CTCAATTTTTGCATCATTGT+TGG | + | chr5.1:78358869-78358888 | None:intergenic | 30.0% |
| !! | TTTTCTTTTGTTTCCTCAAC+AGG | + | chr5.1:78358610-78358629 | None:intergenic | 30.0% |
| !!! | ACATGACATGTTTTAATCAC+GGG | - | chr5.1:78357808-78357827 | MS.gene38101:CDS | 30.0% |
| !!! | CACATGACATGTTTTAATCA+CGG | - | chr5.1:78357807-78357826 | MS.gene38101:CDS | 30.0% |
| AAATGGTGATTCTGTTGTTG+AGG | - | chr5.1:78358663-78358682 | MS.gene38101:intron | 35.0% | |
| AATTTATCCTTATGAACGGG+AGG | + | chr5.1:78358156-78358175 | None:intergenic | 35.0% | |
| ATTTATCCTTATGAACGGGA+GGG | + | chr5.1:78358155-78358174 | None:intergenic | 35.0% | |
| CGTGATTAAAACATGTCATG+TGG | + | chr5.1:78357809-78357828 | None:intergenic | 35.0% | |
| GTCGAGGAAACAAAAGAAAA+TGG | - | chr5.1:78358646-78358665 | MS.gene38101:intron | 35.0% | |
| TGATCTGAATATGATTCTGC+AGG | - | chr5.1:78358333-78358352 | MS.gene38101:intron | 35.0% | |
| TGGACTCTCAATGATTTCTT+CGG | + | chr5.1:78358428-78358447 | None:intergenic | 35.0% | |
| TGTTGTTAAGGATGAGAATG+TGG | - | chr5.1:78358726-78358745 | MS.gene38101:intron | 35.0% | |
| TTCAGCTGAAGATTCTTCTT+TGG | + | chr5.1:78358473-78358492 | None:intergenic | 35.0% | |
| ! | CTCTACCAATTTCAAGTACT+TGG | - | chr5.1:78357654-78357673 | MS.gene38101:CDS | 35.0% |
| ! | TTCCTCTTTCTTTTCATCCA+AGG | + | chr5.1:78358836-78358855 | None:intergenic | 35.0% |
| ACTCTCAATGATTTCTTCGG+TGG | + | chr5.1:78358425-78358444 | None:intergenic | 40.0% | |
| AGAAGAGAAAAGTGAGGAGA+AGG | - | chr5.1:78358795-78358814 | MS.gene38101:intron | 40.0% | |
| AGCCTTGGATGAAAAGAAAG+AGG | - | chr5.1:78358831-78358850 | MS.gene38101:intron | 40.0% | |
| ATGTCATGTGGAAGTAATGC+AGG | + | chr5.1:78357797-78357816 | None:intergenic | 40.0% | |
| CAAACCTTTGCTTAAAGCTG+AGG | - | chr5.1:78358498-78358517 | MS.gene38101:intron | 40.0% | |
| CTCTGACTAATATGGACACA+TGG | + | chr5.1:78358086-78358105 | None:intergenic | 40.0% | |
| TACAGCAGAGGAAACAATGA+AGG | - | chr5.1:78358561-78358580 | MS.gene38101:intron | 40.0% | |
| TGTCATGTGGAAGTAATGCA+GGG | + | chr5.1:78357796-78357815 | None:intergenic | 40.0% | |
| !! | CTCAGCTTTAAGCAAAGGTT+TGG | + | chr5.1:78358500-78358519 | None:intergenic | 40.0% |
| !! | TTTGCCTCAGCTTTAAGCAA+AGG | + | chr5.1:78358505-78358524 | None:intergenic | 40.0% |
| AAATCGCGTCACGTTGTAAC+CGG | - | chr5.1:78357891-78357910 | MS.gene38101:CDS | 45.0% | |
| AGCCTCAGAAGAGAAAAGTG+AGG | - | chr5.1:78358789-78358808 | MS.gene38101:intron | 45.0% | |
| CGGTGATTCAGTTAATGTCG+AGG | - | chr5.1:78358630-78358649 | MS.gene38101:intron | 45.0% | |
| CTCAACAGGTTCCTTTGTCT+CGG | + | chr5.1:78358596-78358615 | None:intergenic | 45.0% | |
| GCTTAAAGCTGAGGCAAAAG+AGG | - | chr5.1:78358507-78358526 | MS.gene38101:intron | 45.0% | |
| GGAAGAACAAACCGAGACAA+AGG | - | chr5.1:78358582-78358601 | MS.gene38101:intron | 45.0% | |
| GGTGGTAGAAGAAGTTCTTG+TGG | - | chr5.1:78358528-78358547 | MS.gene38101:intron | 45.0% | |
| TAAAGCTGAGGCAAAAGAGG+TGG | - | chr5.1:78358510-78358529 | MS.gene38101:intron | 45.0% | |
| TCTGTCTCAGATTCTTCAGC+TGG | + | chr5.1:78358703-78358722 | None:intergenic | 45.0% | |
| TGAGGAGAAGGTTGAGAAAG+AGG | - | chr5.1:78358807-78358826 | MS.gene38101:intron | 45.0% | |
| TGATAACTGAGCAGTGTCGT+CGG | + | chr5.1:78358383-78358402 | None:intergenic | 45.0% | |
| TGGTAGAGTTACCTCAACAG+TGG | + | chr5.1:78357642-78357661 | None:intergenic | 45.0% | |
| TTCTGTTGTTGAGGCTGTTC+AGG | - | chr5.1:78358672-78358691 | MS.gene38101:intron | 45.0% | |
| ! | TCTTTGGTTTCTGATGCTGC+TGG | + | chr5.1:78358457-78358476 | None:intergenic | 45.0% |
| !! | CTCCTCACTTTTCTCTTCTG+AGG | + | chr5.1:78358794-78358813 | None:intergenic | 45.0% |
| CGAGACAAAGGAACCTGTTG+AGG | - | chr5.1:78358594-78358613 | MS.gene38101:intron | 50.0% | |
| CTCAAACCCTCCCGTTCATA+AGG | - | chr5.1:78358146-78358165 | MS.gene38101:CDS | 50.0% | |
| GGATGAGAATGTGGTAGCTG+AGG | - | chr5.1:78358735-78358754 | MS.gene38101:intron | 50.0% | |
| GGCTGTGAAAGATACAGCAG+AGG | - | chr5.1:78358549-78358568 | MS.gene38101:intron | 50.0% | |
| GGTAGCTGAGGCTGAAACTA+AGG | - | chr5.1:78358747-78358766 | MS.gene38101:intron | 50.0% | |
| GGTTGAGAAAGAGGAAGCCT+TGG | - | chr5.1:78358816-78358835 | MS.gene38101:intron | 50.0% | |
| TCTGATGCTGCTGGTGTGAT+TGG | + | chr5.1:78358448-78358467 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 78357635 | 78358927 | 78357635 | ID=MS.gene38101 |
| chr5.1 | mRNA | 78357635 | 78358927 | 78357635 | ID=MS.gene38101.t1;Parent=MS.gene38101 |
| chr5.1 | exon | 78358913 | 78358927 | 78358913 | ID=MS.gene38101.t1.exon1;Parent=MS.gene38101.t1 |
| chr5.1 | CDS | 78358913 | 78358927 | 78358913 | ID=cds.MS.gene38101.t1;Parent=MS.gene38101.t1 |
| chr5.1 | exon | 78357635 | 78358207 | 78357635 | ID=MS.gene38101.t1.exon2;Parent=MS.gene38101.t1 |
| chr5.1 | CDS | 78357635 | 78358207 | 78357635 | ID=cds.MS.gene38101.t1;Parent=MS.gene38101.t1 |
| Gene Sequence |
| Protein sequence |
