AlfalfaGEDB | MS.gene38129

M. sativa cultivar XinJiangDaYe / MS.gene38129

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38129.t1	RHN58171.1	97.3	111	3	0	16	126	110	220	3.70E-49	204.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38129.t1	G7K0M7	97.3	111	3	0	16	126	95	205	2.7e-49	204.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049350	MS.gene38129	0.807591	4.69E-50	-1.69E-46
MS.gene049711	MS.gene38129	0.80861	2.85E-50	-1.69E-46
MS.gene051719	MS.gene38129	0.808689	2.74E-50	-1.69E-46
MS.gene051720	MS.gene38129	0.821058	4.94E-53	-1.69E-46
MS.gene053536	MS.gene38129	0.808822	2.56E-50	-1.69E-46
MS.gene053538	MS.gene38129	0.810368	1.19E-50	-1.69E-46
MS.gene055537	MS.gene38129	0.804175	2.45E-49	-1.69E-46
MS.gene05894	MS.gene38129	0.81899	1.47E-52	-1.69E-46
MS.gene059424	MS.gene38129	0.802417	5.67E-49	-1.69E-46
MS.gene060519	MS.gene38129	0.816572	5.16E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38129.t1	MTR_5g098480	97.297	111	3	0	16	126	95	205	5.24e-71	211
MS.gene38129.t1	MTR_3g082780	54.464	112	34	4	19	126	39	137	2.44e-18	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38129.t1	AT2G04480	47.674	86	37	1	41	126	170	247	3.93e-17	74.7
MS.gene38129.t1	AT5G35870	39.815	108	50	4	22	126	101	196	9.08e-14	65.1

Find 15 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATAGAGATTGATATATCTA+TGG	0.249513	5.1:-79153975	MS.gene38129:CDS
TTCAAACCTTGAGTACTTGA+TGG	0.316759	5.1:+79153949	None:intergenic
AAATCCAACCAGAAGGTAAC+TGG	0.350877	5.1:+79154462	None:intergenic
TTTCAGTCAACAAAGTCTAA+TGG	0.361434	5.1:-79154041	MS.gene38129:CDS
AAGTTGACAAATGATCTTCT+TGG	0.365749	5.1:+79154236	None:intergenic
TTGATCCATCTGAGTAATCA+TGG	0.491540	5.1:+79154188	None:intergenic
ATAGAGATTGATATATCTAT+GGG	0.504620	5.1:-79153974	MS.gene38129:CDS
CATAGCCATGATTACTCAGA+TGG	0.516223	5.1:-79154193	MS.gene38129:CDS
AGATCATTTGTCAACTTCTG+AGG	0.517436	5.1:-79154230	MS.gene38129:CDS
AGAACTCAATGAAGTGAATG+AGG	0.560804	5.1:-79154008	MS.gene38129:CDS
GATCATTTGTCAACTTCTGA+GGG	0.561459	5.1:-79154229	MS.gene38129:CDS
TGGGATCCATCAAGTACTCA+AGG	0.590653	5.1:-79153955	MS.gene38129:CDS
ATTGAAATTGCACTTCCAAG+TGG	0.592923	5.1:-79154145	MS.gene38129:CDS
ATATCCACATAGTTTCCACT+TGG	0.606905	5.1:+79154130	None:intergenic
ACTTCCAAGTGGAAACTATG+TGG	0.636002	5.1:-79154134	MS.gene38129:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAGAGATTGATATATCTAT+GGG	-	chr5.1:79154460-79154479	MS.gene38129:intron	20.0%
!!	TATAGAGATTGATATATCTA+TGG	-	chr5.1:79154459-79154478	MS.gene38129:intron	20.0%
!	CAGAAGAAAAAGAAGTTATT+TGG	-	chr5.1:79154361-79154380	MS.gene38129:intron	25.0%
!	GAAGAAGAAGTTAATATTGA+TGG	-	chr5.1:79154160-79154179	MS.gene38129:CDS	25.0%
	AAGTTGACAAATGATCTTCT+TGG	+	chr5.1:79154201-79154220	None:intergenic	30.0%
	TTTCAGTCAACAAAGTCTAA+TGG	-	chr5.1:79154393-79154412	MS.gene38129:intron	30.0%
	AAAAACCAAATCCAACCAGA+AGG	+	chr5.1:79153982-79154001	None:intergenic	35.0%
	AGAACTCAATGAAGTGAATG+AGG	-	chr5.1:79154426-79154445	MS.gene38129:intron	35.0%
	AGATCATTTGTCAACTTCTG+AGG	-	chr5.1:79154204-79154223	MS.gene38129:CDS	35.0%
	ATATCCACATAGTTTCCACT+TGG	+	chr5.1:79154307-79154326	None:intergenic	35.0%
	ATCTTTGCTCTACACATCTA+TGG	-	chr5.1:79154003-79154022	MS.gene38129:CDS	35.0%
	ATTGAAATTGCACTTCCAAG+TGG	-	chr5.1:79154289-79154308	MS.gene38129:intron	35.0%
	GATCATTTGTCAACTTCTGA+GGG	-	chr5.1:79154205-79154224	MS.gene38129:CDS	35.0%
	TTCAAACCTTGAGTACTTGA+TGG	+	chr5.1:79154488-79154507	None:intergenic	35.0%
	TTGATCCATCTGAGTAATCA+TGG	+	chr5.1:79154249-79154268	None:intergenic	35.0%
!!!	TCTTTTTTCCAGTTACCTTC+TGG	-	chr5.1:79153964-79153983	MS.gene38129:CDS	35.0%
	AAATCCAACCAGAAGGTAAC+TGG	+	chr5.1:79153975-79153994	None:intergenic	40.0%
	ACTTCCAAGTGGAAACTATG+TGG	-	chr5.1:79154300-79154319	MS.gene38129:intron	40.0%
	CATAGCCATGATTACTCAGA+TGG	-	chr5.1:79154241-79154260	MS.gene38129:CDS	40.0%
!	AGTTACCTTCTGGTTGGATT+TGG	-	chr5.1:79153974-79153993	MS.gene38129:CDS	40.0%
!	TTTTCCAGTTACCTTCTGGT+TGG	-	chr5.1:79153968-79153987	MS.gene38129:CDS	40.0%
	TGGGATCCATCAAGTACTCA+AGG	-	chr5.1:79154479-79154498	MS.gene38129:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	79153937	79154519	79153937	ID=MS.gene38129
chr5.1	mRNA	79153937	79154519	79153937	ID=MS.gene38129.t1;Parent=MS.gene38129
chr5.1	exon	79154471	79154519	79154471	ID=MS.gene38129.t1.exon1;Parent=MS.gene38129.t1
chr5.1	CDS	79154471	79154519	79154471	ID=cds.MS.gene38129.t1;Parent=MS.gene38129.t1
chr5.1	exon	79153937	79154268	79153937	ID=MS.gene38129.t1.exon2;Parent=MS.gene38129.t1
chr5.1	CDS	79153937	79154268	79153937	ID=cds.MS.gene38129.t1;Parent=MS.gene38129.t1

Gene Sequence

>MS.gene38129

ATGGATGGCTCTTTTTGCTTTATTGCTTCTTTTTTCCAGTTACCTTCTGGTTGGATTTGGTTTTTTATCTTTGCTCTACACATCTATGGTAGTAATTTTTTCTACTTTTCTCTATATTATTATTGCATCAAAGCAAAAAAAAGTAAATGTTGTTGATAAATTAGTAGAAGAAAAGTTTCTGAATTCAGATGATGAGAAAAGTAATGAAGCACATGAAGCACAAGAAGAAGAAGTTAATATTGATGGATCAGAAAGTGAAAGCCAAGAAGATCATTTGTCAACTTCTGAGGGATCTGAAGTAGATCATAGCCATGATTACTCAGATGGATCAATTTCTGATGAAGAAAGTCTCATTGAAATTGCACTTCCAAGTGGAAACTATGTGGATATGCAGAAAGATGAATCAGATTACTATAGTTTGCAGCAGAAGAAAAAGAAGTTATTTGGTGATGCACTTTTCAGTCAACAAAGTCTAATGGAGTTGTTATCAGAACTCAATGAAGTGAATGAGGAAGAAAACTTTATAGAGATTGATATATCTATGGGATCCATCAAGTACTCAAGGTTTGAAATTGAAGCATGA

Protein sequence

>MS.gene38129.t1

MDGSFCFIASFFQLPSESESQEDHLSTSEGSEVDHSHDYSDGSISDEESLIEIALPSGNYVDMQKDESDYYSLQQKKKKLFGDALFSQQSLMELLSELNEVNEEENFIEIDISMGSIKYSRFEIEA