AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38134


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38134.t1 MTR_4g109450 75.936 187 41 1 29 211 197 383 2.27e-86 272
MS.gene38134.t1 MTR_4g109450 75.936 187 41 1 29 211 197 383 4.46e-86 272
MS.gene38134.t1 MTR_7g028970 69.519 187 53 1 29 211 197 383 4.61e-78 251
MS.gene38134.t1 MTR_7g012820 59.893 187 71 1 29 211 207 393 1.06e-66 220
MS.gene38134.t1 MTR_7g012820 59.893 187 71 1 29 211 278 464 1.29e-66 221
MS.gene38134.t1 MTR_6g013660 58.824 187 73 1 29 211 274 460 3.44e-65 216
MS.gene38134.t1 MTR_3g106160 57.219 187 76 1 29 211 286 472 1.54e-63 212
MS.gene38134.t1 MTR_3g498725 49.198 187 90 2 29 211 290 475 2.96e-53 183
MS.gene38134.t1 MTR_3g102830 49.733 187 89 2 29 211 295 480 3.29e-53 183
MS.gene38134.t1 MTR_8g100040 49.198 187 90 2 29 211 294 479 5.63e-53 182
MS.gene38134.t1 MTR_3g098310 40.314 191 104 3 29 210 333 522 6.04e-36 134
MS.gene38134.t1 MTR_3g098310 40.314 191 104 3 29 210 333 522 6.99e-36 133
MS.gene38134.t1 MTR_3g461000 36.508 189 114 3 27 211 205 391 2.12e-28 112
MS.gene38134.t1 MTR_6g048860 44.444 81 40 2 37 113 179 258 2.43e-15 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38134.t1 AT1G74310 71.658 187 49 1 29 211 197 383 1.07e-82 262
MS.gene38134.t1 AT1G74310 71.658 187 49 1 29 211 197 383 5.21e-82 261
MS.gene38134.t1 AT4G14670 61.497 187 68 1 29 211 162 348 1.50e-69 223
MS.gene38134.t1 AT5G15450 60.428 187 70 1 29 211 272 458 3.58e-67 222
MS.gene38134.t1 AT2G25140 56.150 187 78 1 29 211 277 463 4.01e-61 205
MS.gene38134.t1 AT5G50920 49.198 187 90 2 29 211 292 477 1.27e-52 181
MS.gene38134.t1 AT3G48870 48.663 187 91 2 29 211 282 467 4.00e-52 180
MS.gene38134.t1 AT3G48870 48.663 187 91 2 29 211 313 498 4.73e-52 180
MS.gene38134.t1 AT3G48870 48.663 187 91 2 29 211 313 498 4.73e-52 180
MS.gene38134.t1 AT3G48870 48.663 187 91 2 29 211 313 498 4.73e-52 180
MS.gene38134.t1 AT5G51070 40.314 191 104 3 29 210 306 495 2.01e-33 126
MS.gene38134.t1 AT3G45450 34.940 166 93 3 24 187 166 318 5.96e-22 92.0

Find 48 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAAATCTTGCTGTTGATT+TGG 0.263142 5.1:-78892636 MS.gene38134:CDS
GGTATCAGAAACAATAAGTT+TGG 0.313980 5.1:+78892319 None:intergenic
AAATCAACAGCAAGATTTCT+AGG 0.316313 5.1:+78892638 None:intergenic
ATTCATCTTGTTCTTAGATC+TGG 0.330680 5.1:-78892497 MS.gene38134:CDS
TAGTCCACGAAGGATACTTA+TGG 0.348995 5.1:+78892298 None:intergenic
TCTGTGTAGCCTTTGCCCTT+TGG 0.360414 5.1:+78893222 None:intergenic
TTGTAGGAGAACCAAGTGTT+AGG 0.398382 5.1:-78892712 MS.gene38134:intron
GATACGGGTGCAGTAGTTGC+CGG 0.400106 5.1:-78892614 MS.gene38134:CDS
GTAGTTGCCGGTGCTAAGTA+TGG 0.402418 5.1:-78892602 MS.gene38134:CDS
ATGGTGTTAGAATTCAAGAT+TGG 0.404484 5.1:-78892253 MS.gene38134:CDS
CAGCAACACTTGAGGAATAT+AGG 0.409498 5.1:-78892397 MS.gene38134:CDS
TTGTCTAGTCAGTGCATAAC+TGG 0.416390 5.1:-78892209 None:intergenic
AGAATATGAAGAGAGATTGA+AGG 0.420776 5.1:-78892576 MS.gene38134:CDS
TTATTTCAGAGGATAGTTAG+AGG 0.437448 5.1:-78892668 MS.gene38134:CDS
GTGCTTCTAGTTGCATAATC+TGG 0.448175 5.1:+78893263 None:intergenic
ATTTGAGAGGAGGTTTCAAC+AGG 0.451749 5.1:-78892351 MS.gene38134:CDS
GTTCTTAGATCTGGACGAAC+GGG 0.469341 5.1:-78892488 MS.gene38134:CDS
ATATAGGAAGTATGTTGAAA+AGG 0.473627 5.1:-78892381 MS.gene38134:CDS
GAGAGCTATGAGGGCGATCA+TGG 0.475096 5.1:-78892272 MS.gene38134:CDS
CTTGCTGTTGATTTGGATAC+GGG 0.477054 5.1:-78892629 MS.gene38134:CDS
TGATTGAGTTGGAATTTGAC+CGG 0.480838 5.1:+78893292 None:intergenic
TCTTGCTGTTGATTTGGATA+CGG 0.482021 5.1:-78892630 MS.gene38134:CDS
AGGAAAAGTGTTGTTGTTGA+AGG 0.487141 5.1:-78892692 MS.gene38134:CDS
TGGACGAACGGGAGGATCGA+TGG 0.493742 5.1:-78892477 MS.gene38134:CDS
TGGTGTTAGAATTCAAGATT+GGG 0.503495 5.1:-78892252 MS.gene38134:CDS
GGAAAAGTGTTGTTGTTGAA+GGG 0.518606 5.1:-78892691 MS.gene38134:CDS
AGCTGTCCACGAGCAAGCAT+TGG 0.522301 5.1:+78892434 None:intergenic
CTCTTGTCAATGTCATCCAA+AGG 0.526294 5.1:-78893238 MS.gene38134:CDS
TTCTTCTCCATACTTAGCAC+CGG 0.539250 5.1:+78892595 None:intergenic
CGTGGACAGCTTAGATGCAT+TGG 0.540444 5.1:-78892422 MS.gene38134:CDS
TTCAAACCAATGCTTGCTCG+TGG 0.546102 5.1:-78892440 MS.gene38134:CDS
ACCGCAATGGTATATTGTCC+CGG 0.552447 5.1:-78893311 None:intergenic
TTGTTGAAGGGTTATTTCAG+AGG 0.553219 5.1:-78892679 MS.gene38134:CDS
TCTTGTCAATGTCATCCAAA+GGG 0.554827 5.1:-78893237 MS.gene38134:CDS
GATACCATAAGTATCCTTCG+TGG 0.560735 5.1:-78892302 MS.gene38134:CDS
TCTGGTGGAGGTGATTGAGT+TGG 0.561730 5.1:+78893281 None:intergenic
AGGATGCAGCATTTGAGAGG+AGG 0.568506 5.1:-78892361 MS.gene38134:CDS
CTAGTTGCATAATCTGGTGG+AGG 0.573867 5.1:+78893269 None:intergenic
CTTCTAGTTGCATAATCTGG+TGG 0.577816 5.1:+78893266 None:intergenic
AAAAGGATGCAGCATTTGAG+AGG 0.584513 5.1:-78892364 MS.gene38134:CDS
TGGACTAAAAGAGAGCTATG+AGG 0.585330 5.1:-78892282 MS.gene38134:CDS
GGACTAAAAGAGAGCTATGA+GGG 0.588633 5.1:-78892281 MS.gene38134:CDS
CAATGTCATCCAAAGGGCAA+AGG 0.607048 5.1:-78893231 MS.gene38134:CDS
TGTTCTTAGATCTGGACGAA+CGG 0.608057 5.1:-78892489 MS.gene38134:CDS
CATTGGTGCAGCAACACTTG+AGG 0.609175 5.1:-78892405 MS.gene38134:CDS
GATTGAGTTGGAATTTGACC+GGG 0.622165 5.1:+78893293 None:intergenic
CTTAGATCTGGACGAACGGG+AGG 0.624484 5.1:-78892485 MS.gene38134:CDS
AAGGCTACACAGAAGTTGTG+TGG 0.686150 5.1:-78893212 MS.gene38134:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAATTTAGAAAGAAG+TGG + chr5.1:78892556-78892575 None:intergenic 20.0%
!!! AAATTTATTGTTCTTGGTTT+TGG + chr5.1:78892497-78892516 None:intergenic 20.0%
! AAGAACAATCATGTTCTTAT+AGG + chr5.1:78892739-78892758 None:intergenic 25.0%
! AAGAACAATCTTATTCTTGT+AGG - chr5.1:78892787-78892806 MS.gene38134:intron 25.0%
! TACAAGAATAAGATTGTTCT+TGG + chr5.1:78892788-78892807 None:intergenic 25.0%
! TATAAGAACATGATTGTTCT+TGG - chr5.1:78892738-78892757 MS.gene38134:intron 25.0%
! TTGACGAAATTTATTGTTCT+TGG + chr5.1:78892503-78892522 None:intergenic 25.0%
!! ATATAGGAAGTATGTTGAAA+AGG - chr5.1:78893134-78893153 MS.gene38134:intron 25.0%
AAACAAATCACCAATTTGAG+AGG + chr5.1:78892377-78892396 None:intergenic 30.0%
AAATCAACAGCAAGATTTCT+AGG + chr5.1:78892880-78892899 None:intergenic 30.0%
AACAATCATGTTCTTATAGG+AGG + chr5.1:78892736-78892755 None:intergenic 30.0%
AGAATATGAAGAGAGATTGA+AGG - chr5.1:78892939-78892958 MS.gene38134:intron 30.0%
ATTCATCTTGTTCTTAGATC+TGG - chr5.1:78893018-78893037 MS.gene38134:intron 30.0%
GGTATCAGAAACAATAAGTT+TGG + chr5.1:78893199-78893218 None:intergenic 30.0%
TATCAATCTTGCTGATGTTA+GGG + chr5.1:78892646-78892665 None:intergenic 30.0%
TTATTTCAGAGGATAGTTAG+AGG - chr5.1:78892847-78892866 MS.gene38134:intron 30.0%
! ATGGTGTTAGAATTCAAGAT+TGG - chr5.1:78893262-78893281 MS.gene38134:CDS 30.0%
! TGGTGTTAGAATTCAAGATT+GGG - chr5.1:78893263-78893282 MS.gene38134:CDS 30.0%
!! GAAAAAGTGTTGTTGTTGAA+GGG + chr5.1:78892701-78892720 None:intergenic 30.0%
!! GAAGGAAATTTTGAAAGAAG+TGG - chr5.1:78892957-78892976 MS.gene38134:intron 30.0%
!! TAGAAATCTTGCTGTTGATT+TGG - chr5.1:78892879-78892898 MS.gene38134:intron 30.0%
!!! CAGGATGATCAATTAGTTTT+GGG - chr5.1:78892340-78892359 MS.gene38134:CDS 30.0%
AAAGAAGTGGAAAAAGCTGA+AGG - chr5.1:78892970-78892989 MS.gene38134:intron 35.0%
CTAACATCAGCAAGATTGAT+AGG - chr5.1:78892645-78892664 MS.gene38134:CDS 35.0%
CTATCAATCTTGCTGATGTT+AGG + chr5.1:78892647-78892666 None:intergenic 35.0%
TCTTGTCAATGTCATCCAAA+GGG - chr5.1:78892278-78892297 MS.gene38134:CDS 35.0%
TGATGTTAGGGTAGTTGATT+TGG + chr5.1:78892634-78892653 None:intergenic 35.0%
TGCTTGAAGATTTGTCAAGA+AGG + chr5.1:78892765-78892784 None:intergenic 35.0%
! ACAACACTTTTCCTAACACT+TGG + chr5.1:78892817-78892836 None:intergenic 35.0%
! TTGTTGAAGGGTTATTTCAG+AGG - chr5.1:78892836-78892855 MS.gene38134:intron 35.0%
!! AGGAAAAGTGTTGTTGTTGA+AGG - chr5.1:78892823-78892842 MS.gene38134:intron 35.0%
!! GCAGGATGATCAATTAGTTT+TGG - chr5.1:78892339-78892358 MS.gene38134:CDS 35.0%
!! GGAAAAAGTGTTGTTGTTGA+AGG + chr5.1:78892702-78892721 None:intergenic 35.0%
!! GGAAAAGTGTTGTTGTTGAA+GGG - chr5.1:78892824-78892843 MS.gene38134:intron 35.0%
!! GGTGATTTGTTTAAACAAGC+TGG - chr5.1:78892385-78892404 MS.gene38134:CDS 35.0%
!! TAGGGTAGTTGATTTGGATA+TGG + chr5.1:78892628-78892647 None:intergenic 35.0%
!! TCTTGCTGTTGATTTGGATA+CGG - chr5.1:78892885-78892904 MS.gene38134:intron 35.0%
!! TGATTGAGTTGGAATTTGAC+CGG + chr5.1:78892226-78892245 None:intergenic 35.0%
!!! ACAACACTTTTTCCAACACA+TGG - chr5.1:78892708-78892727 MS.gene38134:CDS 35.0%
AAAAGGATGCAGCATTTGAG+AGG - chr5.1:78893151-78893170 MS.gene38134:intron 40.0%
ATTCTCGATGCCTCTCAAAT+TGG - chr5.1:78892364-78892383 MS.gene38134:CDS 40.0%
ATTTGAGAGGAGGTTTCAAC+AGG - chr5.1:78893164-78893183 MS.gene38134:intron 40.0%
CAGCAACACTTGAGGAATAT+AGG - chr5.1:78893118-78893137 MS.gene38134:intron 40.0%
CTCTTGTCAATGTCATCCAA+AGG - chr5.1:78892277-78892296 MS.gene38134:CDS 40.0%
GATACCATAAGTATCCTTCG+TGG - chr5.1:78893213-78893232 MS.gene38134:CDS 40.0%
GGACTAAAAGAGAGCTATGA+GGG - chr5.1:78893234-78893253 MS.gene38134:CDS 40.0%
TAGTCCACGAAGGATACTTA+TGG + chr5.1:78893220-78893239 None:intergenic 40.0%
TGGACTAAAAGAGAGCTATG+AGG - chr5.1:78893233-78893252 MS.gene38134:CDS 40.0%
TGTTCTTAGATCTGGACGAA+CGG - chr5.1:78893026-78893045 MS.gene38134:intron 40.0%
TTATAGGAGGAACCATGTGT+TGG + chr5.1:78892723-78892742 None:intergenic 40.0%
TTCTTCTCCATACTTAGCAC+CGG + chr5.1:78892923-78892942 None:intergenic 40.0%
TTGTAGGAGAACCAAGTGTT+AGG - chr5.1:78892803-78892822 MS.gene38134:intron 40.0%
! AAGTGTTGTTGTTGAAGGGT+TGG + chr5.1:78892847-78892828 None:intergenic 40.0%
! CTTCTAGTTGCATAATCTGG+TGG + chr5.1:78892252-78892271 None:intergenic 40.0%
! GTGCTTCTAGTTGCATAATC+TGG + chr5.1:78892255-78892274 None:intergenic 40.0%
!! CTTGCTGTTGATTTGGATAC+GGG - chr5.1:78892886-78892905 MS.gene38134:intron 40.0%
!! GATTGAGTTGGAATTTGACC+GGG + chr5.1:78892225-78892244 None:intergenic 40.0%
AAGGCTACACAGAAGTTGTG+TGG - chr5.1:78892303-78892322 MS.gene38134:CDS 45.0%
CAATGTCATCCAAAGGGCAA+AGG - chr5.1:78892284-78892303 MS.gene38134:CDS 45.0%
CTAGTTGCATAATCTGGTGG+AGG + chr5.1:78892249-78892268 None:intergenic 45.0%
GTTCTTAGATCTGGACGAAC+GGG - chr5.1:78893027-78893046 MS.gene38134:intron 45.0%
TGTGGTGACACTCATTTAGC+AGG - chr5.1:78892321-78892340 MS.gene38134:CDS 45.0%
TTCAAACCAATGCTTGCTCG+TGG - chr5.1:78893075-78893094 MS.gene38134:intron 45.0%
TTGGCTCAGAGGATAGTTAG+AGG + chr5.1:78892678-78892697 None:intergenic 45.0%
! AGCTCTCTTTTAGTCCACGA+AGG + chr5.1:78893230-78893249 None:intergenic 45.0%
!!! GTTCTTGGTTTTGGTCGAAC+TGG + chr5.1:78892488-78892507 None:intergenic 45.0%
AAGCTGGTGTGCTTGCTTCA+TGG - chr5.1:78892401-78892420 MS.gene38134:CDS 50.0%
AGCTGGTGTGCTTGCTTCAT+GGG - chr5.1:78892402-78892421 MS.gene38134:CDS 50.0%
AGGATGCAGCATTTGAGAGG+AGG - chr5.1:78893154-78893173 MS.gene38134:intron 50.0%
CATTGGTGCAGCAACACTTG+AGG - chr5.1:78893110-78893129 MS.gene38134:intron 50.0%
CGTGGACAGCTTAGATGCAT+TGG - chr5.1:78893093-78893112 MS.gene38134:intron 50.0%
TCTGGTGGAGGTGATTGAGT+TGG + chr5.1:78892237-78892256 None:intergenic 50.0%
TCTGTGTAGCCTTTGCCCTT+TGG + chr5.1:78892296-78892315 None:intergenic 50.0%
! GTAGTTGCCGGTGCTAAGTA+TGG - chr5.1:78892913-78892932 MS.gene38134:intron 50.0%
!! TTGTTGAAGGGTTGGCTCAG+AGG + chr5.1:78892689-78892708 None:intergenic 50.0%
!!! CTTGGTTTTGGTCGAACTGG+AGG + chr5.1:78892485-78892504 None:intergenic 50.0%
AGCTGTCCACGAGCAAGCAT+TGG + chr5.1:78893084-78893103 None:intergenic 55.0%
CTTAGATCTGGACGAACGGG+AGG - chr5.1:78893030-78893049 MS.gene38134:intron 55.0%
GAGAGCTATGAGGGCGATCA+TGG - chr5.1:78893243-78893262 MS.gene38134:CDS 55.0%
GATACGGGTGCAGTAGTTGC+CGG - chr5.1:78892901-78892920 MS.gene38134:intron 55.0%
TGGACGAACGGGAGGATCGA+TGG - chr5.1:78893038-78893057 MS.gene38134:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 78892210 78893327 78892210 ID=MS.gene38134
chr5.1 mRNA 78892210 78893327 78892210 ID=MS.gene38134.t1;Parent=MS.gene38134
chr5.1 exon 78893213 78893327 78893213 ID=MS.gene38134.t1.exon1;Parent=MS.gene38134.t1
chr5.1 CDS 78893213 78893327 78893213 ID=cds.MS.gene38134.t1;Parent=MS.gene38134.t1
chr5.1 exon 78892210 78892728 78892210 ID=MS.gene38134.t1.exon2;Parent=MS.gene38134.t1
chr5.1 CDS 78892210 78892728 78892210 ID=cds.MS.gene38134.t1;Parent=MS.gene38134.t1
Gene Sequence

>MS.gene38134

ATGGTATATTGTCCCGGTCAAATTCCAACTCAATCACCTCCACCAGATTATGCAACTAGAAGCACTTCTCTTGTCAATGTCATCCAAAGGGCAAAGGCTACACAGAAGTTGTGTGGTGACACTCATTTAGCAGGATGATCAATTAGTTTTGGGAATTCTCGATGCCTCTCAAATTGGTGATTTGTTTAAACAAGCTGGTGTGCTTGCTTCATGGGTTGAATCAGAAGCTGAAAAGCTTCATTGTAAAGAAAAAGTTGAAAGTGCTTCTTGATCCTCCAGTTCGACCAAAACCAAGAACAATAAATTTCGTCAATGAAAAGAATAATCTTTTCTTCAGCTTTTTCCACTTCTTTCTAAATTTTCTTTAATCTCTCTTCAAAATCTTCACTACACTTAGCAACAGCAACTAATGCATCCATATCCAAATCAACTACCCTAACATCAGCAAGATTGATAGGAACATCACCTCTAACTATCCTCTGAGCCAACCCTTCAACAACAACACTTTTTCCAACACATGGTTCCTCCTATAAGAACATGATTGTTCTTGGTCCTTCTTGACAAATCTTCAAGCACCAAGAACAATCTTATTCTTGTAGGAGAACCAAGTGTTAGGAAAAGTGTTGTTGTTGAAGGGTTATTTCAGAGGATAGTTAGAGGCGATGTTCCTAGAAATCTTGCTGTTGATTTGGATACGGGTGCAGTAGTTGCCGGTGCTAAGTATGGAGAAGAATATGAAGAGAGATTGAAGGAAATTTTGAAAGAAGTGGAAAAAGCTGAAGGAAATGTTATTCTTTTCAATGACGAAATTCATCTTGTTCTTAGATCTGGACGAACGGGAGGATCGATGGATGCTACTAATCTTTTCAAACCAATGCTTGCTCGTGGACAGCTTAGATGCATTGGTGCAGCAACACTTGAGGAATATAGGAAGTATGTTGAAAAGGATGCAGCATTTGAGAGGAGGTTTCAACAGGTTTATGTGTCCAAACTTATTGTTTCTGATACCATAAGTATCCTTCGTGGACTAAAAGAGAGCTATGAGGGCGATCATGGTGTTAGAATTCAAGATTGGGCTATTGTTGTTGCAACACAGTTGTCTAGTCAGTGCATAACTG

Protein sequence

>MS.gene38134.t1

MVYCPGQIPTQSPPPDYATRSTSLVNVIQRAKATQKLCGEPSVRKSVVVEGLFQRIVRGDVPRNLAVDLDTGAVVAGAKYGEEYEERLKEILKEVEKAEGNVILFNDEIHLVLRSGRTGGSMDATNLFKPMLARGQLRCIGAATLEEYRKYVEKDAAFERRFQQVYVSKLIVSDTISILRGLKESYEGDHGVRIQDWAIVVATQLSSQCIT